WO2019136938A1 - Protéine accase mutante permettant à une plante d'avoir une résistance aux herbicides, et son application - Google Patents

Protéine accase mutante permettant à une plante d'avoir une résistance aux herbicides, et son application Download PDF

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WO2019136938A1
WO2019136938A1 PCT/CN2018/093006 CN2018093006W WO2019136938A1 WO 2019136938 A1 WO2019136938 A1 WO 2019136938A1 CN 2018093006 W CN2018093006 W CN 2018093006W WO 2019136938 A1 WO2019136938 A1 WO 2019136938A1
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plant
accase
gene
seq
herbicide
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Chinese (zh)
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张保龙
王金彦
凌溪铁
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江苏省农业科学院
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Definitions

  • the invention belongs to the field of plant protein and plant herbicide resistance, relates to ACCase mutant protein which has herbicide resistance to plants and application thereof; in particular, the invention relates to rice acetyl-CoA carboxylase (ACCase) mutein,
  • ACCase rice acetyl-CoA carboxylase
  • the mutein can confer properties to plants, especially rice anti-acetyl-CoA carboxylase inhibitor herbicides.
  • the present invention discloses nucleotide and amino acid sequences of the muteins and their use in the field of plant herbicide resistance.
  • Farmland weeds are one of the leading causes of crop yield reduction. Compared with traditional methods such as cultivation, artificial weeding and mechanical weeding, the use of chemical herbicides is recognized as the most efficient, simple and economical treatment method for controlling weeds in farmland.
  • Acetyl-CoA Carboxylase is a key enzyme that catalyzes the synthesis of plant fatty acids in plant metabolism and is a target for herbicides discovered in 1958.
  • ACCase belongs to the type I (type I) of Biotin containing enzyme and is found in most organisms, mainly including bacteria, yeast, fungi, plants, animals and humans. It has been determined that there are two isoforms of ACCase in plants, mainly in plastids and cytosols, and their functional regions are structurally very different.
  • ACCase in most plant plastids has four subunits: a biotin carboxyl carrier-protein (BCCP), a biotin carboxylase (BC), and a carboxyl transferase (carboxyl transferase).
  • BCCP biotin carboxyl carrier-protein
  • BC biotin carboxylase
  • CT carboxyl transferase
  • CT two subunits CT ⁇ and CT ⁇ , which are covalently linked.
  • the ACCase in most plant cytosols belongs to the same type in yeast, rat and human. Its one polypeptide chain contains all four subunits of prokaryotic ACCase, and the order is BC, BCCP, CT ⁇ , CT ⁇ . 3 functional domains (CT ⁇ and CT ⁇ count a functional domain).
  • the inhibition of ACCase by herbicides is mainly through inhibition of CT, which leads to inhibition of acyl lipid biosynthesis, affecting cell membrane permeability, causing leakage of metabolites, resulting in plant death, thereby achieving the purpose of controlling weeds.
  • ACCase herbicides include Aryloxyphenoxypropanoates (APP), Cyclohexanedione oximes (CHD) and Phenylpyrazoline (DEN). Because of its high selectivity, it can prevent one-year or perennial grass weeds after planting, and it is transmitted in plants. It has the advantages of high efficiency, low toxicity, long application period, safety to crops, etc. These herbicides develop rapidly. With the increasing variety and rapid expansion of the use area, it has become the third important herbicide in the world.
  • APP Aryloxyphenoxypropanoates
  • CHD Cyclohexanedione oximes
  • DEN Phenylpyrazoline
  • ACCase catalyzes the formation of malonyl-CoA from acetyl-CoA. This reaction is the first critical step in the total synthesis of fatty acids. It is also the rate-limiting step. The reaction catalyzed by ACCase is actually a two-step reaction consisting of three subunits. . In the first step, ATP is first supplied with energy to bicarbonate to produce an activated carboxyl group (carboxylic acid, phosphoric acid complex), and then the carboxyl group is linked to the urea ring N1 of biotin in BCCP under the action of BC to form a carboxylated organism.
  • the carboxyl group on the biotin urea ring N1 is transferred to the methyl group of the acetyl-CoA to produce malonyl-CoA, and the process does not require other auxiliary energy.
  • Synthetic malonyl-CoA is involved in biological processes such as de novo synthesis of fatty acids.
  • ACCase has two main types of inhibitors, including aryloxy oxypropionate (APP) and cyclohexanedione (CHD), and several herbicide varieties have been developed, such as herbicide and turfgrass. Propargyl, ketone, clethethene and the like. As early as the 1960s and 1970s, APP and CHD herbicides were widely used in farmland weeds, but with long-term and large-area use, weeds quickly developed resistance to these two herbicides. Sexually, up to 35 weeds have been found in 26 countries to develop resistance to these two types of inhibitors.
  • APP aryloxy oxypropionate
  • CHD cyclohexanedione
  • the technical problem to be solved by the present invention is to provide a rice ACCase mutant protein.
  • a technical problem to be solved by the present invention is to provide a nucleic acid or gene encoding a rice ACCase mutant protein.
  • the technical problem to be solved by the present invention is to provide an expression cassette, a recombinant vector or a cell containing the rice ACCase mutant gene described above.
  • a technical problem to be solved by the present invention is to provide a method for obtaining a herbicide-resistant plant. Specifically, a method for enhancing the tolerance of plants, plant tissues, plant cells to at least one herbicide, the herbicide interfering with the activity of the ACCase enzyme.
  • herbicide resistance herein refers to an anti-(resistant) acetyl-CoA carboxylase-type herbicide.
  • the method for enhancing the resistance of a plant, a plant tissue or a plant cell to a herbicide according to the present invention can be carried out by a method of transformation or hybridization, selfing and vegetative propagation, or can be obtained by chemical mutagenesis or genetic site-directed mutagenesis.
  • the altered plant has any one of the nucleotide sequence of the present invention, SEQ ID No: 1, SEQ ID No: 3, SEQ ID No: 5, SEQ ID No: 7, or SEQ ID No: 9, the mutation Subsequent plants have one or more of the above-described mutated nucleotide sequences.
  • EMS mutagenesis treatment on the wild-type rice variety Zhenjing 19 (obtained from Zhenjiang Agricultural Science Research Institute, Jiangsu Hilly Region), and conducted long-term and continuous screening of these EMS mutant plants.
  • a series of ACCase muteins including some of the known ACCase muteins and new ACCase muteins described above, are insensitive to ACCase inhibitor herbicides, thereby rendering plants ACCase inhibitor herbicide resistant.
  • the use of the present invention in plant breeding can be used to grow plants having herbicide resistance, especially crops, and the development of these proteins and their coding genes in transgenic or non-transgenic plants such as rice.
  • a rice ACCase mutant protein wherein the amino acid sequence of the ACCase mutant protein has any one or more of the following mutations: it corresponds to rice ACCase Mutations occurred at positions 1792, 2015, 2038, 2052, and 2089 of the amino acid sequence.
  • the amino acid sequence of the amino acid sequence of rice ACCase is mutated from isoleucine to leucine, the amino acid at position 2015 is alanine to valine, and the amino acid at 2038 is derived from tryptophan. It becomes cysteine, the amino acid at position 2052 is mutated from isoleucine to asparagine, and the amino acid at position 2089 is changed from aspartic acid to glycine.
  • amino acid mutated by the ACCase mutant protein of the present invention includes, but is not limited to, the following mutations, and it is also within the scope of the present invention to mutate to other amino acids at the following position, for example, the amino acid at position 1792 is isoammine.
  • the invention also includes a rice ACCase mutant protein comprising:
  • the invention also includes nucleic acids or genes encoding the proteins.
  • the nucleic acid or gene of the present invention comprises:
  • nucleotide sequence which hybridizes under stringent conditions to (a) a defined nucleotide sequence and which encodes a protein having acetyl-CoA carboxylase activity;
  • the stringent conditions are: highly stringent hybridization conditions in which a membrane is washed at 65 ° C for 15 minutes in a solution of 0.1 x SSC and 0.1% SDS.
  • Nucleotides in which the ACCase mutant gene of the present invention is mutated include, but are not limited to, the following mutations, and it is also within the scope of the present invention to mutate to other nucleotides at the following positions, for example, A mutation is T, C, G; or C mutation to T, A, G; or T mutation to A, C, G; or G mutation to T, C, A, etc. are within the scope of the present invention.
  • the rice ACCase gene has one or more of the following mutations: nucleotide 5374 of the rice ACCase gene, nucleotide 6044 of the rice ACCase gene, The mutation was carried out at nucleotide position 6114 of the rice ACCase gene, nucleotide 6155 of the rice ACCase gene, and nucleotide 6266 of the rice ACCase gene.
  • the rice ACCase gene has one or several mutations: a mutation in the 5374th nucleotide of the rice ACCase gene from A to T, and a 6042 in the rice ACCase gene.
  • the nucleotide is mutated from C to T, and the nucleotide at position 6114 of the rice ACCase gene is mutated from G to T, and the nucleotide at position 6155 of the ACCase gene in rice is mutated from T to A, and the second in the rice ACCase gene.
  • the 6266 nucleotide is mutated from A to G.
  • the invention also includes an expression cassette, a recombinant vector or a cell comprising the nucleic acid or gene.
  • the present invention also encompasses the use of the rice ACCase mutant protein, nucleic acid or gene, expression cassette, recombinant vector or cell in plant herbicide resistance.
  • the herbicide is one or more selected from the group consisting of quizalofop, halofloxacin, clethethene and flufenoxacillin.
  • the present invention also includes a method of obtaining a herbicide-resistant plant, comprising the steps of:
  • the method comprises a cristr gene editing, transgene, hybridization, backcrossing or vegetative propagation step.
  • the invention also includes a method of identifying a plant, wherein the plant is a plant comprising the nucleic acid or gene, a plant expressing the protein, or a plant obtained by the method, comprising the steps of:
  • the present invention also encompasses a herbicide-protecting agent made from the rice ACCase mutant protein.
  • the invention also includes a method of controlling weeds comprising: applying an effective amount of a herbicide to a field in which the crop is grown, the crop comprising the nucleic acid or gene or the expression cassette, recombinant vector or cell,
  • the herbicide is one or more of quizalofop, halofloxacin, clethethene, and flufenoxacil.
  • the present invention also encompasses a method for protecting a plant from damage caused by a herbicide, comprising: applying an effective amount of a herbicide to a field in which the crop is grown, the crop comprising the nucleic acid or gene or the described
  • the expression cassette and the recombinant vector are introduced into the plant, and the introduced plant produces a herbicide resistance protein, and the herbicide is one or more of quizalofop, halofloxacin, ketoxifen, and flufenoxacil. .
  • the wild-type gene of the present invention is derived from the scorpion 19 of rice.
  • mutant plant of the scorpion 19 of the present invention has quizalofop- herbicide resistance, and the nucleotide sequence or amino acid sequence of the mutant gene of the scorpion 19 mutant plant of the present invention can confer rice plants when expressed in plants. Increased tolerance to acetyl-CoA carboxylase herbicides.
  • the present invention obtains a herbicide-resistant rice plant ACCase-5374 mutant, ACCase-6044 mutant, ACCase-6114 mutant, ACCase-6155 mutant, ACCase-6266 by mutation of Zhenjing 19. Mutants, the five plants were deposited with the China Center for Type Culture Collection (CCTCC).
  • CTCC China Center for Type Culture Collection
  • the numbering of the mutant gene sequence of the scorpion 19 of the present invention and the corresponding nucleotide and amino acid sequences thereof are: a mutation which mutates the nucleotide 5374 of the ACCase gene of the scorpion 19 from A to T, respectively.
  • the ACCase-5374 mutant has the nucleotide sequence of SEQ ID No: 1 and the amino acid sequence of SEQ ID No: 2;
  • the mutant at the 6044th nucleotide of the ACCase gene of the scorpion 19 was changed from C to T, and the ACCase-6044 mutant had the nucleotide sequence of SEQ ID No. :3, the amino acid sequence thereof is shown as SEQ ID No: 4;
  • the mutant of the 6th and 114th nucleotides of the rice ACCase gene of the scorpion 19, which is mutated from G to T is named as ACCase-6114 mutant, and the nucleotide sequence of the ACCase-6114 mutant ACCase gene is SEQ ID No. :5, the amino acid sequence thereof is shown as SEQ ID No: 6;
  • the mutant of the 6155th nucleotide of the rice ACCase gene of the scorpion 19 was renamed as ACCase-6155 mutant, and the ACCase-6155 mutant ACCase gene has the nucleotide sequence of SEQ ID No. :7, the amino acid sequence thereof is shown as SEQ ID No: 8.
  • the mutation of the 6266th nucleotide of the rice ACCase gene of Zhenjing 19 from A to G was named as ACCase-6266 mutant, and the ACCase gene of ACCase-6266 mutant had the nucleotide sequence of SEQ. ID No: 9, the amino acid sequence of which is shown in SEQ ID No: 10;
  • the nucleotide sequence of the wild type ACCase gene of the scorpion 19 is shown in SEQ ID No: 11, and the amino acid sequence thereof is shown in SEQ ID No. 12.
  • a herbicide-resistant rice plant ACCase-5374 mutant, ACCase-6044 mutant, ACCase-6114 mutant, ACCase-6155 mutant, ACCase-6266 mutant were obtained by random mutation.
  • the plants are deposited in the China Center for Type Culture Collection (CCTCC).
  • Figure 1 shows resistant rice mutants obtained by quizalofop herbicide screening
  • Figure 2 shows the full-length results of ACCase gene amplification of 5 rice herbicide-resistant mutants; the first lane is Marker; the molecular weight of Marker is 15kb, 8kb, 5kb, 3kb, 1kb, 1.5kb, 1kb, 750bp from top to bottom. 500 bp, the second lane is the DNA of the wild type scorpion 19, the third lane is the DNA of the herbicide resistant mutant plant (ACCase-5374 mutant), and the fourth lane is the herbicide resistant mutant plant (ACCase-6044 mutant).
  • lane 5 is the DNA of the herbicide-tolerant mutant plant (ACCase-6114 mutant)
  • lane 6 is the DNA of the herbicide-tolerant mutant plant (ACCase-6155 mutant)
  • lane 7 is the herbicide-resistant mutant plant. DNA of (ACCase-6266 mutant).
  • Zhenzhen No. 19 (Gongjiang Agricultural Science Research Institute of Jiangsu Hilly Region) (this is M0, soaked in water for 2 hours) 120kg divided 6 times with 0.4-0.6% (w / w) ethyl methanesulfonate (EMS) room temperature Soak for 6-9 hours, shake the seeds every 1 hour; discard the EMS solution, tap the seeds for 5 times, 5 minutes each time, then rinse the seeds with tap water overnight, plant the field the next day, and carry out conventional fertilizer management. (This is M1). After the plants are mature, the seeds are mixed, dried, and preserved in winter. Sowing the fields the following year.
  • EMS ethyl methanesulfonate
  • Example 2 Obtainment of the wild type Accase full-length gene of Rice Zhenjing 19
  • NCBI Nippon ACCase gene
  • the PCR amplification reaction procedure uses a two-step process, annealing and extension together, using 68 degrees.
  • the ACCase-5374 mutant ACCase gene sequence the 5374th nucleotide of the nucleotide sequence is mutated from A to T, resulting in the mutation of the amino acid sequence 1792 of the encoded amino acid sequence from isoleucine to leucine, Its nucleotide sequence is shown in SEQ ID No: 1, and its encoded amino acid sequence is shown as SEQ ID No: 2;
  • the ACCase-6044 mutant ACCase gene sequence whose nucleotide sequence of nucleotide 6044 is mutated from C to T, causes the amino acid sequence of the amino acid sequence of the encoded amino acid sequence to change from alanine to proline.
  • the nucleotide sequence is shown in SEQ ID No: 3, and the encoded amino acid sequence is shown in SEQ ID No: 4;
  • the ACCase-6114 mutant ACCase gene sequence whose nucleotide sequence of nucleotide sequence 6114 is mutated from G to T, causes the 2038th amino acid of the encoded amino acid sequence to change from tryptophan to cysteine. Its nucleotide sequence is shown in SEQ ID No: 5, and its encoded amino acid sequence is shown as SEQ ID No: 6.
  • the ACCase-6155 mutant ACCase gene sequence the nucleotide sequence of position 6155 from T to A, resulting in the amino acid sequence of the encoded amino acid sequence of the 2052 amino acid from isoleucine to asparagine, its nucleotide The sequence is shown in SEQ ID No: 7, and the encoded amino acid sequence is shown in SEQ ID No: 8.
  • the ACCase-6266 mutant ACCase gene sequence the nucleotide sequence of position 6266 from A to G, resulting in its encoded amino acid sequence 2089 amino acid from aspartic acid to glycine, its nucleotide sequence is SEQ As shown in ID No: 9, the encoded amino acid sequence is shown in SEQ ID No: 10.
  • the ACCase-5374 mutant of the present invention is classified as rice seed zhen19-21 (Oryza sativa Japonica Group zhen19-21), and the material has been deposited with the China Center for Type Culture Collection (CCTCC) on December 20, 2017. : Wuhan University Depository Center, Wuchang District, Wuhan City, Hubei City (opposite to the First affiliated Primary School of Wuhan University), Zip Code: 430072, with the accession number CCTCC No: P201803.
  • CTCC China Center for Type Culture Collection
  • the ACCase-6044 mutant of the present invention is classified as rice seed zhen19-60 (Oryza sativa Japonica Group zhen19-60), and the material has been deposited with the China Center for Type Culture Collection (CCTCC) on December 20, 2017. : Wuhan University Depository Center, Wuchang District, Wuhan City, Hubei City (opposite to the First affiliated Primary School of Wuhan University), Zip Code: 430072, and the deposit number is CCTCC No: P201804.
  • CTCC China Center for Type Culture Collection
  • the ACCase-6114 mutant of the present invention is classified as rice seed zhen19-26 (Oryza sativa Japonica Group zhen19-26), and the material has been deposited with the China Center for Type Culture Collection (CCTCC) on December 20, 2017. : Wuhan University Depository Center, Wuchang District, Wuhan City, Hubei City (opposite to the First affiliated Primary School of Wuhan University), Zip Code: 430072, with the accession number CCTCC No: P201805.
  • CTCC China Center for Type Culture Collection
  • the ACCase-6155 mutant of the present invention is classified as rice seed zhen19-38 (Oryza sativa Japonica Group zhen19-38), and the material has been deposited with the China Center for Type Culture Collection (CCTCC) on December 20, 2017. : Wuhan University Depository Center, Wuchang District, Wuhan City, Hubei City (opposite to the First affiliated Primary School of Wuhan University), Zip Code: 430072, with the accession number CCTCC No: P201806.
  • CTCC China Center for Type Culture Collection
  • the ACCase-6266 mutant of the present invention is classified as rice seed zhen19-7 (Oryza sativa Japonica Group zhen19-7), and the material has been deposited with the China Center for Type Culture Collection (CCTCC) on December 20, 2017. : Wuhan University Depository Center, Wuchang District, Wuhan City, Hubei City (opposite to the First affiliated Primary School of Wuhan University), Zip Code: 430072, with the accession number CCTCC No: P201807.
  • CTCC China Center for Type Culture Collection
  • the five mutant rice lines identified as homozygous mutants in Example 3 were planted and sown, and when the rice was grown to the three-leaf stage, the acetyl-CoA carboxylase inhibitor herbicide quinhefa was sprayed separately.
  • Table 1 shows that the five mutant rice of the present invention is more resistant to ACCase-inhibiting herbicide than the wild type, and the percentage of damage is lower than 60%. This indicates that the herbicide-resistant mutants we screened were indeed able to stably inherit the resistance to anti-acetyl-CoA carboxylase inhibitor herbicides.
  • the specific method is as follows: the female parent is artificially emascated and bagged, and when the male plant is flowering, the pollen in the paternal anther is shaken out, pollinated to the female stigma, repeated twice, and the seed is matured and then harvested. F1 with hybrids. The harvested F1 hybrids were propagated for one generation, and the F2 generation population was harvested.
  • the F2 generation population of M021 and M098 hybrids identified plants with homozygous ACCase-5374 and ACCase-6044 mutations; the M2 and M138 hybrid F2 populations were identified with Plants homozygous for ACCase-5374 and ACCase-6144 mutations; plants with homozygous ACCase6044 and ACCase-6144 mutations were identified in the F2 population of M098 and M138 hybrids.

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Abstract

L'invention concerne une protéine d'Acétyl-CoA Carboxylase (ACCase) mutante de riz. La séquence d'acides aminés de la protéine d'ACCase mutante a une ou plusieurs mutations dans la 1792 ème position, la 2015 ème position, la 2038 ème position, la 2052 ème position et la 2089 ème position de la séquence d'acides aminés de l'ACCase de riz. L'invention concerne également un acide nucléique ou un gène codant pour la protéine. Une plante exprimant la protéine mutante a une résistance (tolérance) à un herbicide d'ACCase. Après l'application de 3 mL de quizalofop-éthyle/L d'eau (2 fois la concentration recommandée) à un plant de riz à trois feuilles, la plante peut toujours croître et grainer.
PCT/CN2018/093006 2018-01-12 2018-06-27 Protéine accase mutante permettant à une plante d'avoir une résistance aux herbicides, et son application WO2019136938A1 (fr)

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CN201810030101.1A CN108359646A (zh) 2018-01-12 2018-01-12 使植物具有除草剂抗性的ACCase突变型蛋白及其应用

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CN109082416B (zh) * 2018-08-22 2021-10-26 江苏省农业科学院 具有除草剂抗性的ACCase突变型基因和蛋白及其应用
CN109371000B (zh) * 2018-10-10 2021-10-26 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN112824526A (zh) * 2019-11-18 2021-05-21 安徽省农业科学院水稻研究所 一种水稻ACCase突变型蛋白及相应基因
CN112813064A (zh) * 2019-11-18 2021-05-18 安徽省农业科学院水稻研究所 一种创制高抗稳定的内源抗除草剂水稻的方法
CN113005103A (zh) * 2019-12-21 2021-06-22 科稷达隆生物技术有限公司 一组抗除草剂蛋白及其在植物育种中的应用
CN115786285A (zh) * 2020-09-23 2023-03-14 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
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