WO2019103165A1 - 바이오 센서 - Google Patents
바이오 센서 Download PDFInfo
- Publication number
- WO2019103165A1 WO2019103165A1 PCT/KR2017/013252 KR2017013252W WO2019103165A1 WO 2019103165 A1 WO2019103165 A1 WO 2019103165A1 KR 2017013252 W KR2017013252 W KR 2017013252W WO 2019103165 A1 WO2019103165 A1 WO 2019103165A1
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- separation membrane
- filler
- blood
- blood cell
- cell separation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/087—Single membrane modules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/34—Energy carriers
- B01D2313/345—Electrodes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
- B01D2315/04—Reciprocation, oscillation or vibration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
Definitions
- the present invention relates to a biosensor, and more particularly, to a biosensor capable of easily passing a plasma that is difficult to pass through a blood cell separation membrane due to surface tension.
- 1 is a configuration diagram of a conventional biosensor.
- a conventional biosensor includes a blood cell separation membrane 100, a microfluidic channel 110, a lower substrate 120, and a spacer 130.
- FIG. 1 (a) is a view of the biosensor as viewed from above, and
- FIG. 1 (b) is a view of a case of A-A 'as a cross section.
- Blood cells contained in the blood are filtered by the hemocyte separation membrane 100, and when the plasma component passes through the hemocyte separation membrane 100, the passed plasma components flow through the microfluidic channel 110.
- a problem to be solved by the present invention is to provide a biosensor capable of easily passing plasma, which is difficult to pass through the hemocyte separation membrane due to its surface tension.
- Another object to be solved by the present invention is to provide a blood cell separating apparatus, in which a filler having a hydrophilic treatment is disposed between a blood cell separation membrane and a lower substrate, and an electrode is disposed in the filler, And to provide a blood glucose measurement device capable of directly measuring blood glucose levels.
- an electrode may be disposed on the filler, and a portion where the filler contacts the hemocyte separation membrane may be formed of an insulator.
- an electrode is formed on a side portion of the filler.
- an empty space may be provided in the filler so that a plasma component passing through the blood cell separation membrane flows along the empty space.
- the surface tension can be reduced by applying piezoelectric or ultrasonic vibration to the filler.
- the filler has a porous structure and the surface of the filler is hydrophilic.
- the present invention provides a hemocyte separation membrane for separating hemocytes from blood and passing plasma components thereof; A lower substrate for storing a plasma component passed through the blood cell separation membrane; And a plurality of pillars for connecting between the hemocyte separation membrane and the lower substrate, wherein the plurality of fillers are positioned at regular intervals on a rim of a space formed between the hemocyte separation membrane and the lower substrate, And an electrode is disposed on at least one of the electrodes.
- a space between the plurality of fillers is filled with hydrogel.
- a hemocyte separation membrane comprising: a hemocyte separation membrane for separating blood cells from blood and passing plasma components; A microfluidic channel through which the plasma component passed from the hemocyte separation membrane flows; A lower substrate through which the plasma component passed from the blood cell separation membrane flows along the microfluidic channel; A filler that connects between the blood cell separation membrane and the lower substrate, and pushes up the blood cell separation membrane by a certain distance; An electrode disposed in the filler; A housing having a receiving space formed therein and having an insertion port through which the electrode can be inserted; Two or more contact pins disposed in the inner space of the housing and contacting the electrodes; And a signal processing unit for measuring and analyzing an amount of current transferred through the electrode to convert the blood sugar into a numerical value.
- the blood glucose measurement using the blood glucose measurement device is performed at least three times, and when the change in the measured blood glucose value is within a certain range, the plasma is collected between the electrode and the electrode, And a plasma saturation determination unit.
- the present invention it is possible to easily pass plasma that is difficult to pass through the hemocyte separation membrane due to surface tension.
- a hydrophilic-treated filler between a blood cell separation membrane and a lower substrate and arranging an electrode in the filler, plasma passing through the blood cell separation membrane can be measured directly through the electrode disposed in the filler have.
- 1 is a configuration diagram of a conventional biosensor.
- FIG. 2 is a configuration diagram of a biosensor according to a preferred embodiment of the present invention.
- FIG. 3 is a configuration diagram of a biosensor according to another preferred embodiment of the present invention.
- Fig. 4 shows various structures of the pillars shown in Figs. 2 and 3 (a).
- FIG. 5 is a graph comparing plasma release times of a biosensor including a filler and a biosensor without a filler according to an embodiment of the present invention.
- the present invention provides a hemocyte separation membrane for separating hemocytes from blood and passing plasma components thereof; A microfluidic channel through which the plasma component passed from the hemocyte separation membrane flows; A lower substrate through which the plasma component passed from the blood cell separation membrane flows along the microfluidic channel; And a filler connecting between the blood cell separation membrane and the lower substrate, wherein an electrode is disposed on the filler, and the filler pushes up the blood separation membrane a predetermined distance.
- FIG. 2 is a configuration diagram of a biosensor according to a preferred embodiment of the present invention.
- the biosensor according to the present embodiment includes a hemocyte separation membrane 200, a microfluidic channel 210, a lower substrate 220, a spacer 230, and a filler 240.
- FIG. 2 (a) is a view of the biosensor as viewed from above
- FIG. 2 (b) is a view of a cross section of A-A '.
- the hemocyte separation membrane 200 separates blood cells and plasma while the components having different sizes in the blood proceed in the direction of gravity.
- the hemocytes are trapped in the pores constituting the hemocyte separation membrane 200 and separated, and the plasma component passes through the hemocyte separation membrane 200.
- the material of the hemocyte separation membrane may be polysulfone.
- plasma components are meant to include plasma and serum.
- the plasma primer Before receiving the blood to be examined, the plasma primer may be supplied to the lower surface of the blood cell separation membrane 200 to lower the surface tension. It is preferable to use a solution composed of sodium, magnesium and potassium values similar to plasma.
- the microfluidic channel 210 is a passage through which the plasma component passed from the hemocyte separation membrane 200 flows.
- the flow velocity of the plasma component may be varied by at least one of the length, width, and volume of the microfluidic channel 210.
- the lower substrate 220 allows the plasma component passed from the blood cell separation membrane 200 to flow along the microfluidic channel 210.
- the lower substrate 220 is preferably hydrophilic.
- the spacer 230 is positioned between the lower substrate 220 and the blood cell separation membrane 200 and separates the lower substrate 220 from the blood cell separation membrane 200.
- the filler 240 connects between the hemocyte separation membrane 200 and the lower substrate 220. At this time, it is preferable that the pillars 240 push up the hemocyte separation membrane 200 a predetermined distance d.
- plasma components are collected between the blood cell separation membrane 200 and the lower substrate 220, so that the plasma components are easily contacted to the electrodes. .
- the surface where the filler 240 and the blood cell separation membrane 200 are in contact with each other may be formed of an insulator, and the filler 240 may have a porous structure.
- an empty space may be provided in the filler 240 so that the plasma component passing through the blood cell separation membrane 200 flows along the empty space.
- the surface of the filler 240 can be subjected to a hydrophilic treatment so as to lower the surface tension and piezoelectric vibrations or ultrasonic vibrations having a natural frequency of the filler can be applied to the filler 240.
- the filler 240 itself is made of a hydrophilic material or the surface of the filler is hydrophilic.
- the hydrophilic material constituting the filler 240 may be a hydrogel.
- FIG. 3 is a configuration diagram of a biosensor according to another preferred embodiment of the present invention.
- FIG. 3 (a) shows that the filler 240 is positioned at intervals of a space formed between the blood cell separation membrane 200 and the lower substrate 220 at regular intervals.
- the filler 240 shown in FIG. 3 (a) is formed between the hemocyte separation membrane 200 and the lower substrate 220 while the filler is located at the center of the hemocyte separation membrane 200
- the plasma component passing through the hemocyte separation membrane 200 is prevented from passing between the filler and the filler due to the surface tension. As a result, the plasma component is collected inside the filler.
- an electrode is disposed on a plurality of pillars disposed around the hemocyte separation membrane 200 so that the plasma component collected inside the filler can be analyzed.
- electrodes may be formed on the side portions of the plurality of pillars.
- hydrogels are filled between the plurality of fillers.
- 3B shows that the one side of the pillars 240 is in contact with the periphery of the hemocyte separation membrane 200 and the lower substrate 220 and has a hollow cylindrical structure. At this time, it is preferable that an electrode is disposed on the inner surface of the cylindrical structure.
- 3C shows that the one side of the pillars 240 is in contact with the periphery of the hemocyte separation membrane 200 and the lower substrate 220 and has a rectangular columnar structure inside. At this time, it is preferable that an electrode is disposed on the inner surface of the square column structure.
- the plasma component filled in the pillars of the cylindrical structure or the square column structure may be further provided.
- Fig. 4 shows various structures of the pillars shown in Figs. 2 and 3 (a).
- FIG. 4 (a) shows various embodiments in which the pillars 240 are cylindrical columns.
- FIG. 4 (a) there is a case where the plasma component passing through the blood cell separation membrane 200 falls down on the outer wall of the filler of the cylindrical column. Further, when there is a cylindrical space in the inside of the pillar, a conical columnar space whose upper end is narrower than the lower end, or an inverted conical columnar space whose upper end is wider than the lower end, plasma components may be drawn down by gravity It will be possible.
- FIG. 4 (b) shows various embodiments in which the upper end of the pillar 240 is a conical column narrower than the lower end.
- plasma components passing through the blood cell separation membrane 200 comes down on the filler outer wall of the conical column whose upper end is narrower than the lower end.
- plasma components may be lowered by gravity through not only the outer wall of the filler but also the inner wall.
- Fig. 4 (c) shows various embodiments in which the upper end of the pillar 240 is an inverted conical column wider than the lower end.
- plasma components passing through the blood cell separation membrane 200 comes down on the filler outer wall of the inverted conical column whose upper end is wider than the lower end.
- plasma components may be lowered by gravity through not only the outer wall of the filler but also the inner wall.
- FIG. 5 is a graph comparing plasma release times of a biosensor including a filler and a biosensor without a filler according to an embodiment of the present invention.
- the y-axis in FIG. 5 represents the time taken for 4 ⁇ L of plasma to be released when 40 ⁇ L (microliter) of whole blood is injected.
- the top horizontal bar of the box shows the time it takes for the plasma to escape 99%, the top of the box to take 75% of the time, the bottom of the box to take 25% of the plasma, the bottom bar of the box to 1%
- the time taken to escape, the horizontal bars in the box mean the average time.
- the biosensor according to the embodiment of the present invention can be applied not only to the blood sugar measuring device but also to other fine sample measuring devices such as lactose and cholesterol.
- a blood glucose measurement device includes a blood cell separation membrane that separates blood cells from blood, passes plasma components, a microfluidic channel through which the plasma component passes from the blood cell separation membrane, A bottom plate for allowing the component to flow along the microfluidic channel, a pillar for connecting the hemocyte separation membrane and the bottom substrate, a pillar for pushing up the hemocyte separation membrane by a certain distance, an electrode disposed in the pillar, A housing having a housing having an insertion port into which the electrode can be inserted, at least two contact pins disposed in an inner space of the housing and contacting the electrode, and an amount of current transferred through the electrode is measured and analyzed And a signal processing unit for converting the blood sugar into a numerical value.
- the plasma saturation determination unit determines that the plasma is collected between the electrode and the electrode when the change in the measured blood glucose value is within a predetermined range and the blood glucose measurement using the blood glucose measurement apparatus is performed at least three times. .
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Abstract
Description
Claims (11)
- 혈액으로부터 혈구를 분리하고, 혈장성분을 통과시키는 혈구분리 멤브레인;상기 혈구분리 멤브레인으로부터 통과한 혈장성분이 흐르는 미세유체 채널;상기 혈구분리 멤브레인으로부터 통과한 혈장성분이 상기 미세유체 채널을 따라 흐르도록 하는 하부기판; 및상기 혈구분리 멤브레인과 상기 하부기판 사이를 연결하는 필러를 포함하고,상기 필러에 전극이 배치되고, 상기 필러는 상기 혈구분리 멤브레인을 일정거리만큼 밀어 올린 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러의 측면부에 전극이 형성된 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러가 상기 혈구분리 멤브레인과 접촉하는 부분은 절연체로 형성하는 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러의 내부에 빈 공간을 구비하고, 상기 혈구분리 멤브레인을 통과한 혈장성분이 상기 빈 공간을 따라 흐르도록 하는 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러에 압전 또는 초음파 진동을 가하여 표면장력을 저하시키는 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러 표면이 친수성인 것을 특징으로 하는 바이오 센서.
- 제1 항에 있어서,상기 필러는 다공성 구조를 갖는 것을 특징으로 하는 바이오 센서.
- 혈액으로부터 혈구를 분리하고, 혈장성분을 통과시키는 혈구분리 멤브레인;상기 혈구분리 멤브레인으부터 통과한 혈장성분이 보관되는 하부기판; 및상기 혈구분리 멤브레인과 상기 하부기판 사이를 연결하는 복수의 필러를 포함하고,상기 복수의 필러는 상기 혈구분리 멤브레인과 상기 하부기판 사이에 형성되는 공간의 테두리에 일정 간격마다 위치하고, 상기 복수의 필러 중 적어도 하나 이상에는 전극이 배치되는 것을 특징으로 하는 바이오 센서.
- 제8 항에 있어서,상기 복수의 필러들 사이의 공간은 수화젤(Hydrogel)을 채우는 것을 특징으로 하는 바이오 센서.
- 혈액으로부터 혈구를 분리하고, 혈장성분을 통과시키는 혈구분리 멤브레인;상기 혈구분리 멤브레인으로부터 통과한 혈장성분이 흐르는 미세유체 채널;상기 혈구분리 멤브레인으로부터 통과한 혈장성분이 상기 미세유체 채널을 따라 흐르도록 하는 하부기판;상기 혈구분리 멤브레인과 상기 하부기판 사이를 연결하며, 상기 혈구분리 멤브레인을 일정거리만큼 밀어 올린 필러;상기 필러에 배치되는 전극;내부에 수용공간이 형성되고, 일측에는 상기 전극이 삽입될 수 있는 삽입구가 형성된 하우징;상기 하우징의 내부공간에 배치되어 상기 전극에 접촉하는 2개 이상의 접촉핀; 및상기 전극을 통해 전송되는 전류량을 측정 및 분석하여 혈당을 수치적인 값으로 변환하는 신호처리부를 포함하는 혈당 측정장치.
- 제10 항에 있어서,상기 혈당측정장치를 이용한 혈당측정을 적어도 3번 이상 실시하고, 측정되는 혈당값의 변화가 일정 범위 이내인 경우에 혈장이 전극과 전극 사이에 모여 포화된 것으로 판단하는 혈장포화여부 판단부를 더 포함하는 혈당 측정장치.
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AU2022201994B2 (en) * | 2022-01-27 | 2023-11-30 | Speclipse, Inc. | Apparatus and method for separating fluid |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995024641A1 (en) * | 1994-03-08 | 1995-09-14 | Andcare, Inc. | Electrochemical immunoassay methods |
KR101293938B1 (ko) * | 2009-12-30 | 2013-08-08 | 라이프스캔, 인코포레이티드 | 충전 시간을 사용하여 생체 센서의 정확도를 개선하기 위한 시스템, 장치, 및 방법 |
KR20130122457A (ko) * | 2012-04-30 | 2013-11-07 | (주) 마이크로프랜드 | 혈액 분리를 위한 칩을 구비한 진단 기구 |
KR20150009745A (ko) * | 2013-07-17 | 2015-01-27 | 주식회사 미코 | 바이오 센서 칩 |
KR20160129937A (ko) * | 2015-04-30 | 2016-11-10 | 계명대학교 산학협력단 | 휴대용 인슐린 저항성 진단장치 및 이를 이용한 진단방법 |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4891104A (en) * | 1987-04-24 | 1990-01-02 | Smithkline Diagnostics, Inc. | Enzymatic electrode and electrode module and method of use |
JPH0643131A (ja) * | 1992-07-22 | 1994-02-18 | Fujitsu Ltd | バイオセンサ、バイオセンサ集合体、及び、その製造方法 |
US5352351A (en) * | 1993-06-08 | 1994-10-04 | Boehringer Mannheim Corporation | Biosensing meter with fail/safe procedures to prevent erroneous indications |
US5494562A (en) * | 1994-06-27 | 1996-02-27 | Ciba Corning Diagnostics Corp. | Electrochemical sensors |
DE19753847A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Analytisches Testelement mit Kapillarkanal |
US6033866A (en) * | 1997-12-08 | 2000-03-07 | Biomedix, Inc. | Highly sensitive amperometric bi-mediator-based glucose biosensor |
EP1114997B1 (en) * | 1999-12-28 | 2009-09-30 | ARKRAY, Inc. | Blood testing tool |
US6726818B2 (en) * | 2000-07-21 | 2004-04-27 | I-Sens, Inc. | Biosensors with porous chromatographic membranes |
WO2002010734A1 (fr) * | 2000-07-31 | 2002-02-07 | Matsushita Electric Industrial Co., Ltd. | Biocapteur |
CN1180259C (zh) * | 2000-07-31 | 2004-12-15 | 松下电器产业株式会社 | 生物传感器 |
JP3808393B2 (ja) * | 2002-03-28 | 2006-08-09 | 富士写真フイルム株式会社 | 血液検査ユニットおよび血液検査装置 |
CN1720438A (zh) * | 2002-11-29 | 2006-01-11 | 日本电气株式会社 | 分离设备和分离方法 |
WO2006105110A2 (en) * | 2005-03-29 | 2006-10-05 | Inverness Medical Switzerland Gmbh | Assay device and methods |
JP2009500601A (ja) * | 2005-06-29 | 2009-01-08 | オックスフォード バイオセンサーズ リミテッド | 電極プリコンディショニング |
GB0525997D0 (en) * | 2005-12-21 | 2006-02-01 | Oxford Biosensors Ltd | Micro-fluidic structures |
CN101490562B (zh) * | 2006-07-10 | 2012-12-19 | 株式会社日立高新技术 | 液体输送设备 |
JP4844318B2 (ja) * | 2006-09-22 | 2011-12-28 | 住友ベークライト株式会社 | マイクロ流路デバイス |
JP5145749B2 (ja) * | 2007-03-30 | 2013-02-20 | セイコーエプソン株式会社 | 検出素子 |
US20100203621A1 (en) * | 2007-07-25 | 2010-08-12 | Panasonic Corporation | Biosensor |
EP2238232B1 (en) * | 2008-01-29 | 2019-08-14 | California Institute of Technology | Method and apparatus for microfiltration to perform cell separation |
US9801575B2 (en) * | 2011-04-15 | 2017-10-31 | Dexcom, Inc. | Advanced analyte sensor calibration and error detection |
KR101032691B1 (ko) * | 2009-04-17 | 2011-05-06 | (주)디지탈옵틱 | 신속한 혈구분리가 가능한 질병진단용 바이오센서 |
CN102023142A (zh) * | 2009-09-15 | 2011-04-20 | 福华电子股份有限公司 | 生物微流道检测装置及其分子检测方法 |
KR101046156B1 (ko) * | 2010-03-12 | 2011-07-04 | 주식회사 나노엔텍 | 혈구 분리 칩 |
CN104066513B (zh) * | 2012-01-24 | 2017-10-03 | 皇家飞利浦有限公司 | 具有过滤单元的分析盒 |
JP5708878B2 (ja) * | 2012-03-15 | 2015-04-30 | 株式会社村田製作所 | バイオセンサおよびその製造方法 |
EP2931427B1 (en) * | 2012-12-13 | 2022-02-09 | Siemens Healthineers Nederland B.V. | Fluidic system with fluidic stop |
US9380973B2 (en) * | 2013-04-15 | 2016-07-05 | Becton, Dickinson And Company | Biological fluid sampling transfer device and biological fluid separation and testing system |
US10368832B2 (en) * | 2013-06-28 | 2019-08-06 | Koninklijke Philips N.V. | Lung tissue identification in anatomically intelligent echocardiography |
JP6071785B2 (ja) * | 2013-07-12 | 2017-02-01 | 株式会社堀場製作所 | 濃度測定装置 |
KR101517594B1 (ko) * | 2013-10-16 | 2015-05-04 | (주)타스컴 | 바이오 센서 |
JP6782565B2 (ja) * | 2015-06-05 | 2020-11-11 | 日東電工株式会社 | バイオセンサチップ及びバイオセンサ装置 |
KR102274967B1 (ko) * | 2017-06-09 | 2021-07-07 | 후지필름 가부시키가이샤 | 마이크로 유로 디바이스 |
-
2017
- 2017-11-21 JP JP2020544712A patent/JP7005780B2/ja active Active
- 2017-11-21 WO PCT/KR2017/013252 patent/WO2019103165A1/ko active Application Filing
- 2017-11-21 KR KR1020177033697A patent/KR101933457B1/ko active IP Right Grant
- 2017-11-21 EP EP17932851.3A patent/EP3714787A4/en active Pending
- 2017-11-21 CN CN201780096793.3A patent/CN111343919B/zh active Active
-
2020
- 2020-05-12 US US15/930,073 patent/US11529629B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995024641A1 (en) * | 1994-03-08 | 1995-09-14 | Andcare, Inc. | Electrochemical immunoassay methods |
KR101293938B1 (ko) * | 2009-12-30 | 2013-08-08 | 라이프스캔, 인코포레이티드 | 충전 시간을 사용하여 생체 센서의 정확도를 개선하기 위한 시스템, 장치, 및 방법 |
KR20130122457A (ko) * | 2012-04-30 | 2013-11-07 | (주) 마이크로프랜드 | 혈액 분리를 위한 칩을 구비한 진단 기구 |
KR20150009745A (ko) * | 2013-07-17 | 2015-01-27 | 주식회사 미코 | 바이오 센서 칩 |
KR20160129937A (ko) * | 2015-04-30 | 2016-11-10 | 계명대학교 산학협력단 | 휴대용 인슐린 저항성 진단장치 및 이를 이용한 진단방법 |
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