WO2019098352A1 - Cosmetic composition, screening method, and cosmetic method - Google Patents

Cosmetic composition, screening method, and cosmetic method Download PDF

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Publication number
WO2019098352A1
WO2019098352A1 PCT/JP2018/042581 JP2018042581W WO2019098352A1 WO 2019098352 A1 WO2019098352 A1 WO 2019098352A1 JP 2018042581 W JP2018042581 W JP 2018042581W WO 2019098352 A1 WO2019098352 A1 WO 2019098352A1
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Prior art keywords
extract
ipl
cosmetic composition
acid
agent
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PCT/JP2018/042581
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French (fr)
Japanese (ja)
Inventor
佐藤　康成
由佳 杉原
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ロート製薬株式会社
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Priority to JP2019554433A priority Critical patent/JPWO2019098352A1/en
Publication of WO2019098352A1 publication Critical patent/WO2019098352A1/en
Priority to JP2024011621A priority patent/JP2024045354A/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the present invention relates to cosmetic compositions, screening methods, cosmetic methods and IPL radiation-like agents.
  • IPL Intelligent Pulse Light
  • Photofacial registered trademark
  • LiLight Lienis One
  • IPL refers to light in a wide wavelength range that is cut short wavelength harmful to the skin such as ultraviolet light.
  • the light of a plurality of wavelengths in a wavelength range of 500 nm to 1200 nm which widely includes visible light and infrared light.
  • various symptoms can be effectively improved.
  • a filter according to the application light in these wavelength ranges can be used to limit the wavelength range and waveform, and irradiation can be performed according to skin conditions. It is said that this treatment is effective for the reduction of firmness, the opening of pores, the color unevenness of the skin, the dullness and the like, and is also effective for the alleviation of the aging symptoms of the entire face.
  • the IPL contains light of a plurality of wavelengths, it is possible to irradiate the whole face with multiple irradiations as described above, instead of local irradiation such as only spot and hokuro as in conventional laser treatment. It is believed that the problem can be effectively improved.
  • the present invention has been made in view of such a situation, and it elucidates the mechanism of action of light treatment and sufficiently exerts a skin condition improving effect on skin whitening, anti-aging, etc., as with IPL irradiation. And, it is an object of the present invention to provide a new idea cosmetic composition which can be easily used by many users, and a new cosmetic method using it.
  • IPL irradiation to epidermal cells suppresses the expression of genes involved in melanin synthesis and melanosome transport, and genes involved in turnover. It was found that expression was promoted. That is, it is considered that the whitening mechanism by IPL irradiation is to act on the above-mentioned genes involved in melanin synthesis, melanosome transport and turnover, and by using this finding, the composition for external use such as cosmetics is similar to IPL irradiation.
  • the gist of the present invention is as follows.
  • a cosmetic composition containing an IPL radiation-like agent [2] The cosmetic composition according to [1], wherein the IPL irradiation-like agent at least contains a plant extract containing flavonoid as an active ingredient. [3] The above plant extract is Cucurbitaceae, Asteraceae, Leguminosae, Rosaceae, Salixaceae, Salixaceae, Fernaceae, Citrus Department, Citrusaceae, Anchovyaceae, Anchovyaceae, Acacia, Asperiaceae, Monimimiaceae, Aescariaceae, The cosmetic composition according to [2], which is an extract of at least one kind of plant selected from the group consisting of a family of plants belonging to the family Laminaceae, the family Laminaceae, a family of mallows and a family of ginger.
  • the melanin related molecule is at least one selected from the group consisting of OCA2, SLC45A2 and RAB27A2.
  • the plant extract is an eggplant extract, an artichoke extract, a licorice root extract, a bilberry fruit extract, a black rosewood bark extract, a meria azalea lacta leaf extract, a mandarin orange peel extract, a yukinoshita extract, a horse mackerel seed extract, a borage seed extract
  • the cosmetic composition according to any one of the above.
  • any one of [1] to [7], which contains at least one member selected from the group consisting of a turnover accelerator (excluding the above-mentioned IPL irradiation-like active agent), an anti-glycation component and a whitening active component A cosmetic composition according to any one of the preceding claims. [9] The cosmetic composition according to any one of [1] to [8], which is for skin tone improver. [10] A screening method of a component for improving skin tone, comprising screening a component effective for improving skin tone by using a gene whose expression is changed by irradiating IPL to skin cells as an index.
  • the screening method according to [10] which comprises at least the steps of allowing a test substance to act on skin culture cells, and measuring the gene expression in the skin culture cells.
  • a cosmetic method for improving or improving the skin condition which comprises using an IPL irradiation-like agent.
  • An IPL radiation-like active agent comprising, as an active ingredient, at least one plant extract selected from the group consisting of Tambrissa tricophila leaf extract, rosemary extract, burdock extract, and altea extract.
  • An IPL irradiation-like agent comprising, as an active ingredient, at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae and blue algae.
  • a cosmetic composition of a novel idea that sufficiently achieves the same skin condition improving effect as IPL irradiation with respect to skin whitening, anti-aging, etc. and can be easily used by many users. Can be provided. In addition, new cosmetic methods using it can also be provided.
  • the cosmetic composition of the present invention contains an IPL radiation-like agent.
  • the IPL irradiation-like agent refers to a composition capable of giving the same effect as when the subject is irradiated with IPL. This is a composition that can replace light treatment such as IPL radiation, ie a cosmetic composition for IPL radiation substitution.
  • an IPL irradiation-like active agent some effects may be given among the effects obtained by irradiating IPL to a subject.
  • the cosmetic composition of the present invention is effective even if it is used alone, but it is also possible to improve the effect of IPL irradiation and maintain it for a long time by combining it with the light treatment such as IPL irradiation.
  • the effects obtained by irradiating the subject with IPL include, for example, the expression regulation effect of melanin-related molecules in skin cells, the skin whitening effect of all faces, and the improvement effect of skin tone.
  • improvement effects improvement effect of skin tone
  • melanin related molecules include OCA2, SLC45A2, RAB27A2, HB-EGF and the like.
  • expression of OCA2, SLC45A2 and RAB27A2 is regulated in a repressing direction by IPL irradiation.
  • the cosmetic composition of the present invention may be a cosmetic composition for suppressing gene expression of at least one selected from the group consisting of OCA2, SLC45A2 and RAB27A2, or a cosmetic composition for promoting gene expression of HB-EGF.
  • the cosmetic composition of the present invention can be said to be a cosmetic composition for skin tone improvement and / or skin tone improvement similar to IPL irradiation because it suppresses or promotes the above-mentioned gene expression.
  • the cosmetic composition of the present invention may contain other optional components in addition to the above IPL radiation-like active agent as long as the effects of the present invention are not impaired.
  • the following describes the IPL radiation-like agent and other optional components.
  • the IPL irradiation-like agent is not particularly limited as long as it is a substance that can give the same effect as when IPL irradiation is given to a subject, but, for example, various plant extracts etc. are effective What is contained as a component is mentioned, and it is preferable that it is a thing which at least contains the plant extract containing flavonoid. Moreover, it is also preferable that it is a thing containing a seaweed extract at least.
  • Examples of plant extracts containing flavonoids include Cucurbitaceae, Asteraceae, Leguminosae, Rosaceae, Salixaceae, Salixaceae, Neridaceae, Citrusaceae, Citrusaceae, Pisidiomycidae, Salamanders, Asinaceae, Monimimiaceae, Aquilinaceae Extracts of plants such as azaleas, botans, mints, mallows, gingers and the like can be mentioned.
  • amacha persimmon extract for example, amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, white syllable bark extract, melia azalea lacta leaf extract, mandarin orange peel extract, yukinoshita extract, horse mackerel seed extract, borage Examples thereof include fruit extract, hop flower extract, Tambrissa tricophila leaf extract, Hybodaidai flower extract, hydrolysed soybean peptide, white willow bark extract, lice tree extract, rosemary extract, burdock extract, altea extract and the like.
  • a leaf, a flower, a fruit, a root, a stem, a skin, a branch, a fruit, a peel, a seed etc. are mentioned, for example.
  • a plant material may be used as it is or may be dried and used. Plant materials can also be used after being crushed. Moreover, you may use the plant extract marketed as a cosmetics raw material etc.
  • seaweeds used for the above seaweed extract include seaweeds such as brown algae (Gagome kombu, Risei ibu, mitsuishi kombu, macombe, naga kombu, wakame etc.), red algae (eg Tengusa etc.), green algae (Aonori etc.), blue-green algae (spyrulina etc.) And the like extracts.
  • the seaweed raw material may be used as it is or may be dried and used. Plant materials can also be used after being crushed.
  • These seaweeds may be used alone or in combination of two or more. Among these, from the viewpoint of being excellent in the effect of promoting the expression of melanin-related molecules, extracts of brown algae are preferable, extracts of kelp are more preferable, and extracts of gagome comb are more preferable.
  • the melanin-related molecules from the viewpoint of being excellent in the effect of reducing the expression of the OCA2 gene, particularly, artichoke extract, scutellaria root fruit extract, amacha mulberry extract, hydrolyzed soybean peptide (hydrolyzed soybean protein), licorice root
  • artichoke extract scutellaria root fruit extract, amacha mulberry extract, hydrolyzed soybean peptide (hydrolyzed soybean protein), licorice root
  • the extract, the neubara fruit extract, the tambrissa tricophila leaf extract, and the pufferfish flower extract are preferable, and the artichoke extract, the bilberry fruit juice extract, the licorice root extract, and the neurala fruit extract are more preferable.
  • melanin-related molecules particularly from the viewpoint of being excellent in reducing the expression of the SLC45A2 genera extract, licorice root extract, Neubara fruit extract, artichoke extract, hydrolyzed soybean peptide, white willow bark extract, Melia Azadirachta Leaf extract, mandarin orange peel extract, Yukinoshita extract, horse mackerel seed extract, and flower extract are preferable, and amacha mulberry extract, licorice root extract and neubara fruit extract are more preferable.
  • melanin-related molecules particularly from the viewpoint of being excellent in the effect of reducing the expression of RAB27A2, amachar extract, artichoke extract, hydrolyzed soy peptide, licorice root extract, Neubara fruit extract, Yukinoshita extract, Horse mackerel seed extract, tan Preferred are Brissatricophila leaf extract, Fubodaiji flower extract and Izayoibara extract.
  • melanin-related molecules from the viewpoint of the excellent effect of reducing the gene expression of any two or more of OCA2, SLC45A2 and RAB27A2, amacha mulle extract, artichoke extract, hydrolyzed soy peptide, licorice root extract, Neubara fruit extract, Yukinoshita Extract, horseradish seed extract, pufferfish flower extract, white syllable bark extract, mandarin orange peel extract, and Tamburissa Trichophila leaf extract are preferable, and from the viewpoint of being excellent in the effect of reducing the expression of all three genes, Artichoke extract, hydrolyzed soybean peptide, licorice root extract, Neubara fruit extract, Yukinoshita extract, Horse mackerel seed extract, Hybodaige flower extract is more preferable
  • rosemary extract from the viewpoint of being excellent in the effect of promoting the expression of the HB-EGF gene, rosemary extract, seaweed extract, altea extract, albino fruit extract and burdock extract are preferable, seaweed extract,retea extract, Rosemary extract is more preferred.
  • amacha spruce extract as the above-mentioned plant extract in the cosmetic composition of the present invention, amacha spruce extract, artichoke extract, licorice root extract, neubara fruit extract, black radish bark extract, melia azalacilla leaf extract Among them, mandarin orange peel extract, Yukinoshita extract, horseradish seed extract, scutellaria root fruit extract, hop flower extract, Tambrissa tricophila leaf extract, rosemary extract and altea extract are preferable.
  • a brown-alga extract is preferable, a kelp extract is more preferable, and a gagome kombu extract is further more preferable.
  • the content of the IPL irradiation-like agent in the cosmetic composition of the present invention is 0.0001% to 20% by weight when the IPL irradiation-like agent is the plant extract, 0.001 weight % To 10% by weight is preferable, 0.01% to 5% by weight is more preferable, and 0.05% to 3% by weight is further preferable.
  • the cosmetic composition of the present invention may contain the above-mentioned plant extract singly or in combination of two or more. When the cosmetic composition of the present invention contains two or more plant extracts, the total content of the two or more plant extracts is taken as the content of the plant extract.
  • the cosmetic composition unlike the conventional whitening mechanism by tyrosinase activity inhibition etc., has an effect of increasing skin tone by directly acting on genes involved in melanin synthesis and melanosome transport that determine whiteness of the skin. (Skin tone improvement effect).
  • skin tone improvement improves stains, buckwheat, skin dullness and pigmentation associated with aging, and brings it closer to a more natural and even skin color (skin tone improvement) as well as the original skin color. It also includes raising the skin tone of dark skin.
  • a whitening active component for example, a turnover accelerator (except for the IPL irradiation-like active agent of the present invention), an antiglycation component, an antioxidant component, an antiaging component, an antiinflammatory agent Agent, refreshing agent, disinfectant, vitamins, organic acid, moisturizing component, polyhydric alcohol, scrub agent, ultraviolet absorbing component, ultraviolet scattering component, astringent component, peptide or derivative thereof, amino acid or derivative thereof, cleansing component, keratin Soft components, cell activation components, circulation promoting action components and the like can be mentioned.
  • these components may be used alone or in combination of two or more.
  • whitening active ingredients examples include tocopherol, vitamin C and derivatives thereof, arbutin, kojic acid, placenta, ellagic acid, nicotinamide, tranexamic acid and derivatives thereof, hydroquinone, Lucinol (registered trademark), and potassium 4-methoxysalicylic acid. Salts, linoleic acid and derivatives thereof, and the like.
  • turnover promoting agent examples include vitamins to be described later, keratin softness component, cell activation component, blood circulation promoting component and the like.
  • anti-glycation component examples include plant extracts such as budgera akisilalis leaf extract, evening primrose oil, fruits of Amlar, fruit juice or extracts thereof, L-arginine, L-lysine, hydrolysed casein, hydrolyzable tannin , Carnosine and the like.
  • antioxidant component examples include components derived from plants (eg, grape, ginseng, comfrey etc.); proanthocyanidin, tocopherol and derivatives thereof, ascorbic acid and derivatives thereof, hesperidin and derivatives thereof, ergothioneine, sulfite Examples include sodium hydrogen, erythorbic acid and its salts, flavonoids, glutathione and the like.
  • antiaging component examples include hydrolyzed soybean protein, retinoid (retinol and derivatives thereof, retinoic acid, and retinal and the like), pangamic acid, kinetin, ursolic acid, turmeric extract, sphingosine derivative, silicon, silicic acid, N- Methyl-L-serine, mevalonolactone and the like can be mentioned.
  • anti-inflammatory agent examples include allantoin and derivatives thereof, glycyrrhetinic acid and derivatives thereof, glycyrrhizinic acid and derivatives thereof, salicylic acid derivatives, aminocaproic acid, azulene and derivatives thereof, zinc oxide, tocopherol acetate and the like.
  • terpenes such as menthol, camphor, geraniol and the like (these may be d-form, l-form or dl-form); Eucalyptus oil, bergamot oil, peppermint oil, coolmint oil, Examples include essential oils such as spearmint oil and peppermint oil.
  • germicides examples include isopropylmethylphenol, salicylic acid, benzalkonium chloride, ethanol, benzethonium chloride, gluconic acid and derivatives thereof, Photosensitizer No. 101, Photosensitizer No. 201, parabens, phenoxyethanol, piroctoramine, miconazole and the like.
  • the above-mentioned vitamins may be any of water-soluble vitamins and oil-soluble vitamins, for example, vitamin B6s, pantothenic acids, nicotinic acids, vitamin B1s, vitamin B2s, biotins folates, vitamin B12s, Examples thereof include water-soluble vitamin C, oil-soluble vitamin C, vitamin K, and vitamin-like agents such as ferulic acid.
  • organic acids examples include gluconic acid, aspartic acid, aminoethylsulfonic acid, citric acid, glutamic acid, succinic acid, oxalic acid, fumaric acid, propionic acid, malic acid, salicylic acid, glycolic acid, phytic acid, tartaric acid, acetic acid , Lactic acid, and their salts.
  • salts of mineral acids such as sulfuric acid, hydrochloric acid or phosphoric acid
  • salts of organic acids such as maleic acid or methanesulfonic acid
  • alkali metal salts such as sodium or potassium, alkaline earth metal salts, ammonium salts etc.
  • the moisturizing component examples include high molecular weight compounds such as sodium hyaluronate, heparin analogue, sodium chondroitin sulfate, collagen, elastin, keratin, chitin, chitosan, proteoglycan and the like; amino acids such as glycine, aspartic acid and arginine; sodium lactate, Natural moisturizing factors such as urea and sodium pyrrolidonecarboxylate; lipids such as ceramide, cholesterol and phospholipids; plant extracts such as chamomile extract, hamamelis extract, tea extract and shiso extract, and polyhydric alcohols such as glycerin and the like.
  • high molecular weight compounds such as sodium hyaluronate, heparin analogue, sodium chondroitin sulfate, collagen, elastin, keratin, chitin, chitosan, proteoglycan and the like
  • amino acids
  • the polyhydric alcohol preferably has 2 to 10 carbon atoms, and examples thereof include glycerin, diglycerin, triglycerin, propylene glycol, dipropylene glycol, 1,3-butanediol, ethylene glycol, diethylene glycol, isoprene glycol, 1 And 3-butylene glycol, sorbitol, xylitol, erythritol, mannitol, pentanediol, hexanediol, octanediol, decanediol, neopentyl glycol and the like.
  • apricot kernel powder for example, apricot kernel powder, almond shell powder, apricot kernel powder, sodium chloride particles, olive kernel powder, dried seawater particles, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, charcoal powder And husk powder, polyethylene powder, silicic acid anhydride and the like.
  • UV absorbing component examples include ethylhexyl methoxycinnamate, hexyl diethylaminohydroxybenzoylbenzoate, bisethylhexyl oxyphenol methoxyphenyl triazine, ethylhexyl triazone, t-butyl methoxy dibenzoyl methane, octocrylene, polysilicone-15, methylene Bisbenzotriazolyl tetramethylbutylphenol, dimethoxybenzylidene dioxoimidazolidine octylate, phenyl benzimidazole sulfonic acid and the like can be mentioned.
  • Examples of the ultraviolet light scattering component include inorganic compounds such as hydrous silicic acid, zinc silicate, cerium silicate, titanium silicate, titanium oxide, zirconium oxide, cerium oxide, titanium oxide, iron oxide, zinc oxide, silicic anhydride, etc.
  • An inorganic compound coated with an inorganic powder such as hydrous silicic acid, aluminum hydroxide, mica or talc, or compounded with a resin powder such as polyamide, polyethylene, polyester, polystyrene or nylon, and further silicone oil or fatty acid aluminum What was processed by salt etc. is mentioned.
  • astringent component examples include alum, zinc sulfate, aluminum chloride, zinc sulfocarbonate, tannic acid and the like.
  • Examples of the peptide or derivatives thereof include keratinolytic peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin degrading peptides, collagenolytic peptides, hydrolyzed collagen, hydroxypropyl ammonium chloride hydrolyzed collagen, Elastin-degrading peptide, Conchiolin-degrading peptide, Hydrolyzed conquioline, Silk-proteolytic peptide, Hydrolyzed silk, Lauroyl-hydrolyzed silk sodium, Soy-proteolytic peptide, Hydrolyzed soy protein, Wheat protein, Wheat-proteolytic peptide, Hydrolyzed wheat protein, Caseinolytic peptides, acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.) and the like can be mentioned
  • amino acids or derivatives thereof examples include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, glycine, alanine, phenylalanine, ⁇ -alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, Cystine, methionine, leucine, isoleucine, valine, histidine, taurine, ⁇ -aminobutyric acid, ⁇ -amino- ⁇ -hydroxybutyric acid, carnitine, carnosine, creatine and the like.
  • washing component examples include soaps selected from alkali metal salts such as potassium laurate, potassium myristate, potassium palmitate or potassium stearate, alkanolamide salts or amino acid salts, etc .; cocoylglutamate Na, cocoyl methyltaurine Na Amino acid surfactant such as ether sulfate ester salt such as sodium laureth sulfate ether carboxylate salt such as sodium lauryl ether acetate salt sulfosuccinic acid ester salt such as alkyl sulfosuccinic ester Na such as coconut oil fatty acid monoethanolamide, coconut oil Fatty acid alkanolamides such as fatty acid diethanolamide; monoalkyl phosphate ester salts such as sodium lauryl phosphate, polyoxyethylene lauryl ether sodium phosphate; coconut oil fatty acid amido propyl dime L-aminoacetic acid betaine, lauryl dimethylaminoacetic acid betaine, 2-al
  • keratin softness component examples include lactic acid, salicylic acid, glycolic acid, citric acid, malic acid, fruit acid, phytic acid, urea, sulfur and the like.
  • the cell activation component examples include amino acids such as ⁇ -aminobutyric acid; vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids; ⁇ -hydroxy acids such as glycolic acid and lactic acid; tannin, flavonoid, Saponin, photosensitive element No. 301 and the like can be mentioned.
  • amino acids such as ⁇ -aminobutyric acid
  • vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids
  • ⁇ -hydroxy acids such as glycolic acid and lactic acid
  • tannin, flavonoid, Saponin, photosensitive element No. 301 and the like can be mentioned.
  • Examples of the above-mentioned blood circulation promoting active ingredient include plants (eg, Panax ginseng, hornhead, Arnica, Ginkgo biloba, Ginkgo biloba, Ginkgo biloba, Ginkgo biloba, Ginkgo biloba, Chamomile, Roman chamomile, Carrot, Gentian, Burdock, Rice, Hawthorn, Shiitake, Ginger, Hawthorn, Prunus edulis , Senkyu, assembly, thyme, chopsticks, chimpanzee, chili pepper, touki, toonin, spruce, carrot, garlic, butcherbroom, grape, button, marronie, melissa, yuzu, yokuinin, ryokucha, rosemary, rosehip, chimpo, touki, Ingredients derived from spruce, peach, apricot, walnut, corn etc); acetylcholine, ictamol, cantaristinki, gamm
  • the method for producing the cosmetic composition of the present invention is not particularly limited, and the IPL irradiation-like active agent which is an essential component, other components optionally mixed (the above optional components, the base or carrier described later, additives, etc. ) Can be appropriately selected and blended, and can be produced by a conventional method.
  • the cosmetic composition of the present invention is an approach based on the IPL treatment (light treatment) idea of a cosmetologist and used in expectation of the same effect on the entire face as IPL irradiation.
  • skin whitening and skin tone improvement effects can be obtained.
  • skin tone improvement skin tone improvement
  • the cosmetic composition of the present invention can be used as an alternative to IPL radiation and in combination with IPL radiation.
  • the cosmetic composition of the present invention used for such purpose is, for example, a cosmetic solution, a lotion, an emulsion, a cream, a gel cream, a cosmetic mask (face mask), a cosmetic base, a foundation, a sunscreen, a cleansing agent, etc.
  • the composition for external use can be suitably used, and more preferably, it can be used as a skin care product such as a cosmetic solution, a lotion, an emulsion, a cream, a gel cream and the like.
  • the cosmetic composition of the present invention can be a food composition.
  • This food composition can be suitably used as a health food, a nutritional supplement (including a balanced nutritional diet, a supplement, etc.).
  • a nutritional supplement including a balanced nutritional diet, a supplement, etc.
  • health functional food food for specified health use (including disease risk reduction indication, standard standard type)).
  • composition for external use a composition for external use
  • a base or carrier usually used for cosmetics, medicines, quasi-drugs and its essential components and other optional components described above, etc.
  • it mixes with an additive mentioned later according to a conventional method, and it emulsifies or solubilizes as needed, It can be set as the cosmetic composition of various formulation forms.
  • the base or carrier examples include hydrocarbons such as liquid paraffin, squalane, petrolatum, gelled hydrocarbon (plasti base etc.), ozokerite, ⁇ -olefin oligomer, light liquid paraffin, etc .; methyl polysiloxane, cyclic silicone, modified silicone Silicone oils such as silicone resins; Fats such as coconut oil, olive oil, rice bran oil, shea butter, etc. Waxes such as jojoba oil, milows, candelilla wax, lanolin, etc.
  • hydrocarbons such as liquid paraffin, squalane, petrolatum, gelled hydrocarbon (plasti base etc.), ozokerite, ⁇ -olefin oligomer, light liquid paraffin, etc .
  • methyl polysiloxane cyclic silicone, modified silicone Silicone oils such as silicone resins
  • Fats such as coconut oil, olive oil, rice bran oil, shea butter, etc.
  • Waxes such as jojoba oil, milows
  • the base or carrier described above may be used alone or in combination of two or more.
  • the amount used thereof is appropriately selected from the range known to those skilled in the art.
  • the cosmetic composition of the present invention may be a known additive added to cosmetics, pharmaceuticals, quasi-drugs, as long as the effects of the present invention are not impaired, such as surfactants, antioxidants, colorants, pearls
  • a glossing agent, a chelating agent, a pH adjuster, a preservative, a thickener, etc. can be added.
  • These additives may be used alone or in combination of two or more.
  • the surfactant may be any of nonionic surfactant, cationic surfactant, anionic surfactant, amphoteric surfactant and the like, and examples thereof include sorbitan monoisostearate and sorbitan monolaurate. Sorbitan monopalmitate, sorbitan monostearate, diglycerol sorbitan penta-2-ethylhexyl acid, sorbitan fatty acid esters such as diglycerol sorbitan tetra-2-ethylhexyl acid; propylene glycol fatty acid esters such as propylene glycol monostearate; Cured castors of polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil 60 (HCO-60), polyoxyethylene hydrogenated castor oil 80, etc.
  • HCO-40 polyoxyethylene hydrogenated castor oil 40
  • HCO-50 polyoxyethylene hydrogenated castor oil
  • HCO-60 polyoxyethylene
  • antioxidants examples include dibutylhydroxytoluene, butylhydroxyanisole, sorbic acid, sodium sulfite, ascorbic acid, erythorbic acid, L-cysteine hydrochloride and the like.
  • coloring agent an inorganic pigment, a natural pigment
  • chelating agent examples include EDTA ⁇ disodium salt, hydroxyethane diphosphonic acid ⁇ calcium ⁇ disodium salt and the like.
  • pH adjuster examples include inorganic acids (hydrochloric acid, sulfuric acid etc.), organic acids (lactic acid, sodium lactate, citric acid, sodium citrate, sodium citrate, succinic acid, sodium succinate etc.), inorganic bases (potassium hydroxide, hydroxide Sodium etc., organic bases (triethanolamine, diisopropanolamine, triisopropanolamine etc.) and the like.
  • preservative examples include benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, methyl parahydroxybenzoate, phenoxyethanol, capryl hydroxamic acid, pentanediol, hexanediol, octane Alkane diols, such as diol, etc. are mentioned.
  • thickener examples include vinyl thickeners such as polyvinyl alcohol, polyvinyl pyrrolidone and carboxyvinyl polymer, methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose and the like.
  • vinyl thickeners such as polyvinyl alcohol, polyvinyl pyrrolidone and carboxyvinyl polymer, methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose and the like.
  • Cellulose thickeners guar gum, pectin, pullulan, gelatin, locust bean gum, carrageenan, agar, xanthan gum, acrylic acid methacrylic acid alkyl methacrylate copolymer, polyethylene glycol, bentonite, alginic acid, propylene glycol alginate, macrogol, chondroitin sulfate Sodium, hyaluronic acid, sodium hyaluronate, Hydroxyethylmethacrylate / acryloyldimethyltaurinato Na) copolymer, and (ammonium acryloyldimethyltaurate / vinyl pyrrolidone) copolymers and the like.
  • composition for external use The formulation form in the case where the cosmetic composition of the present invention is a composition for external use is not particularly limited, and, for example, a liquid preparation (skin lotion, lotion, serum etc.), cream preparation (milky lotion, cream, gel cream, balm etc.), Gel formulations, jelly formulations, sherbet formulations, aerosol formulations, foam formulations, spray formulations, patches, stick formulations, other ointments, solid formulations and the like can be mentioned. These preparations can be manufactured according to a conventional method, for example, the method described in the 17th revised Japanese Pharmacopoeia General Rules, and the like.
  • agent types such as W / O, O / W, W / O / W, and O / W / O type emulsions can be appropriately selected in consideration of the desired stability, feeling in use, and the like.
  • the patch may be, for example, a non-woven sheet, a gel sheet or the like impregnated with a cosmetic composition, a cosmetic composition, an emulsion composition or the like.
  • the cosmetic composition of the present invention is a food composition
  • the essential components thereof and the other optional components described above, etc. may be used as a base or carrier generally used in food, and, if necessary, fats and oils, emulsifiers, It may be formulated by mixing with food additives such as dispersants, suspending agents, stabilizers, thickeners, sweeteners, colorants, flavors, preservatives, antioxidants, organic acids and the like.
  • the food additive is not limited to the above-described ones and the specific components described later, and can be selected appropriately according to the purpose and necessity.
  • these food additives can be used individually by 1 type or in combination of 2 or more types.
  • other active ingredients can be added to the formulation.
  • the above fats and oils include natural vegetable oils such as peanut oil, cocoa butter, rice germ oil, perilla oil, linseed oil, etc., and hydrogenated oils thereof, glycerides of fatty acids (including medium-chain fatty acids) (glycerides, diglycerides, triglycerides, etc.)
  • glycerides of fatty acids including medium-chain fatty acids
  • the bee wax etc. are mentioned. Among them, beeswax is preferable.
  • emulsifying agent examples include polyethylene glycol, propylene glycol, glycerin, polyhydric alcohol such as sorbitol, sorbitol; glycerin fatty acid ester; sodium carboxymethylcellulose and the like. Among them, glycerin, glycerin fatty acid ester and carboxymethylcellulose sodium are preferable.
  • thickener examples include locust bean gum, guar gum, xanthan gum, tamarind gum, pectin, carrageenan and the like. Among them, xanthan gum is preferred.
  • sucrose, fructose, maltose, erythritol, trehalose, maltitol, licorice extract, stevia processed sweetener, aspartame, acesulfame potassium, sorbitol, xylitol and the like can be mentioned.
  • preservative examples include benzoic acid or a salt thereof, sorbic acid or a salt thereof, butyl parahydroxybenzoate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, ethyl parahydroxybenzoate, methyl parahydroxybenzoate and the like.
  • ascorbic acid sodium ascorbate, ascorbic acid stearic acid ester, sodium ascorbic acid stearic acid ester, ascorbic acid palmitic acid ester, hydrogen sulfite, sodium hyposulfite, sulfur dioxide, calcium EDTA disodium, erythorbic acid Sodium erythorbate, tocopherol and the like.
  • ascorbic acid, ascorbic acid palmitate ester, and tocopherol are preferable.
  • organic acid examples include citric acid, succinic acid, tartaric acid, aspartic acid, lactic acid, malic acid, malonic acid, fumaric acid and maleic acid.
  • the pH of the cosmetic composition of the present invention is usually 3.0 to 8.0, preferably 3.5 to 7.5.
  • this pH can be adjusted, for example by use of the pH adjuster mentioned later.
  • the present invention also includes a method of screening a skin tone improving component and a method of screening a skin tone improving component.
  • the screening method of the present invention is a method characterized by using a gene whose expression is changed by irradiating IPL to skin cells as an indicator, and a step (1) of causing a test substance to act on skin culture cells, the above-mentioned skin culture At least a step (2) of measuring the gene expression in the cell. According to the screening method of the present invention, it is possible to efficiently obtain a skin tone improving component and a skin tone improving component that can obtain the same effect as when IPL irradiation (phototreatment) is received.
  • the skin culture cell is not particularly limited as long as it is a cell population containing melanocytes.
  • epidermal cells, dermal cells, a cultured skin model and the like can be used.
  • As the above-mentioned cultured skin model use of MEL-300A (MatTek) or EFT-400 (MatTek) is more preferable.
  • the test substance is added to the above-mentioned cultured skin cells and cultured for about 2 to 72 hours. As a negative control, one to which no test substance is added is prepared.
  • Step (2) After the above step (1), cells are recovered, RNA is extracted according to a conventional method, and gene expression is analyzed by a DNA microarray method or the like. The gene expression of each of the group to which the test substance was added and the group to which no test substance was added (negative control) in step (1) is matched. At this time, as a gene to be matched, a gene whose fluctuation has been confirmed to occur by irradiating IPL to skin cells is used. Such genes include, for example, OCA2, SLC45A2, RAB27A2, and HB-EGF. These genes are genes found by the present inventors to be suppressed or promoted in expression by IPL irradiation.
  • the test substance in the addition group of the test substance, it is confirmed whether the expression of each gene fluctuates as in the IPL irradiation. And the test substance which changes expression of these genes similarly to the time of IPL irradiation can be determined as the component for skin tone improvement, and the component for skin tone improvement.
  • the present invention also includes cosmetic methods for improving and improving skin conditions, characterized by using an IPL radiation-like agent.
  • the same effect as IPL irradiation can be obtained by using the IPL irradiation-like active agent, and thus, for example, the effect of improving skin whitening and skin tone can be obtained.
  • the improvement effect (skin tone improvement effect) of aging symptoms such as the dullness of the whole face, texture disorder, fine wrinkles, and telangiectasia can be obtained.
  • the description in the term of the above-mentioned cosmetic composition can be applied as it is as the specific description of the above-mentioned IPL irradiation-like agent.
  • the present invention also includes IPL radiation-like agents containing various plant extracts as active ingredients.
  • the plant extract containing a flavonoid and a seaweed extract are preferable.
  • a plant extract containing the above-mentioned flavonoids amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, albino bark extract, Melia azalacilla leaf extract, mandarin orange peel extract, yukinoshita extract, horseradish seed
  • seaweed extract examples include brown algae (gagome kombu, lysiensis, mitsushikonbu, maconbu, nagacomb, wakame etc.), red algae (eg tenegusa etc.), green algae (eg Aonori etc.), blue-green algae (spyrulina etc.) etc. preferable.
  • the IPL irradiation-like active agent of the present invention amacha persimmon extract, artichoke extract, licorice root extract, neybara fruit extract, albino bark extract, Meria azadia lacta leaf extract, mandarin orange peel extract, yukinoshita extract, horse mackerel seeds
  • one containing at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue algae as an active ingredient is preferable, and seaweed extracts of brown algae are preferable. It is more preferable to contain it as an active ingredient.
  • the description of the IPL radiation-like agent in the section of the above cosmetic composition can be applied.
  • the same effect as IPL radiation can be obtained, for example, skin whitening of all faces, skin tone improving effect, all faces dullness, using a cosmetic composition containing this An improvement effect (amelioration effect of skin tone) of aging symptoms such as no firmness, pore appearance and dryness is obtained.
  • the description in the term of the above-mentioned cosmetic composition can be applied as it is as the specific description of the above-mentioned IPL irradiation-like agent.
  • the epidermal model prepared by the above method was irradiated with light (IPL 560 nm) using a filter of ⁇ 560 nm for 3 ms, and then irradiated for 20 ms with another 3 ms, and cells were recovered after 6 hours and 24 hours .
  • IPL irradiation was performed using Photofacial M22 (made by Luminous Corporation).
  • RNA was extracted from each cell according to a conventional method, and gene expression was analyzed by the DNA microarray method.
  • the DNA microarray used Human Gene 2.0 ST Array.
  • OCA2, RAB27A, SLC45A2, and HB-EGF were selected as genes whose expression was changed in epidermal cells by IPL 560 nm irradiation.
  • RNA was extracted by a conventional method, cDNA was synthesized from the RNA by reverse transcription reaction, and the expression amounts of the OCA2, SLC45A2 and RAB27A genes were quantified by quantitative real-time PCR.
  • the ratio to blank was calculated using the obtained value.
  • a blank means the data of the control test by only the culture medium which has not added each extract sample. The results are shown in Tables 1 to 17 below.
  • Test 3 Screening of substances that affect the expression of HB-EGF gene in human keratinocytes Normal human adult epidermal cells (Kurabo: NHEK) using epidermal keratinocyte culture medium (Kurabo: Humedia KG2) The cells were seeded at 40000 cells / well in a 24-well plate (CORNING) and cultured for 1 day. Thereafter, extract samples are prepared with medium to give each concentration, and those which are passed through a filter with a pore size of 0.2 ⁇ m are added at 400 ⁇ l / well to a 24-well plate from which the medium has been removed, and cultured for another 24 hours. The cells were harvested.
  • RNA was extracted by a conventional method, cDNA was synthesized from the RNA by reverse transcription reaction, and the expression level of each gene of HB-EGF was quantified by quantitative real time PCR. The ratio to blank was calculated using the obtained value. In addition, a blank means the data of the control test by only the culture medium which has not added each extract sample. The results are shown in Table 18 below.
  • the cosmetic composition and the cosmetic method of the present invention can also be expected to have an effect (bright skin tone improving effect) to lighten skin with a darker skin tone than originally expected (a cosmetic composition of the present invention can be used as a skin tone improver). Can also be used.
  • the cosmetic composition and the cosmetic method of the present invention can also be expected to have the effect of improving aging symptoms (skin tone improving effect) such as dullness of the entire face, lack of firmness, pore appearance, dryness, etc. it can.
  • Prescription example 1 (Beauty fluid) L-ascorbic acid 10.0 (w / w%) Hydroquinone 0.1 Diethylene glycol monoethyl ether 51.0 Propylene glycol 23.0 Horse mackerel extract 0.1 Neubara Fruit Extract 0.5 Total purified water remaining 100.0%
  • Prescription example 2 (Beauty fluid) 3-O-ethyl ascorbic acid 3.0 (w / w%) Diethylene glycol monoethyl ether 30.0 Propylene glycol 20.0 Glycerin 4.0 Altea extract 1.0 Artichoke extract 10.0 Total purified water remaining 100.0%
  • Prescription example 3 (Skin lotion) Tranexamic acid 1.5 (w / w%) Salicylic acid 0.1 Sodium hyaluronate 0.2 Glycerin 2.0 1,3-butylene glycol 10.0 Polyoxypropylene methyl glucoside 0.2 Acetylated sodium hyaluronate 0.01 Hydroxyethyl cellulose 0.05 Methyl parahydroxybenzoate 0.15 Flavoring agent 0.03 Oil-soluble licorice extract 0.0001 Total purified water remaining 100.0%
  • Prescription example 4 (Skin lotion) Tranexamic acid 1.5 (w / w%) Salicylic acid 0.1 Sodium hyaluronate 0.2 Glycerin 1.0 Diglycerin 0.5 1,3-butylene glycol 5.0 Dipropylene glycol 3.0 Trehalose 0.1 Acetylated sodium hyaluronate 0.01 Hydroxyethyl cellulose 0.05 Methyl parahydroxybenzoate 0.15 Flavoring agent 0.03 Seaweed extract 0.5 Rosemary extract 0.3 Total purified water remaining 100.0%
  • Prescription example 6 (milky lotion) Tranexamic acid 2.0 (w / w%) ⁇ -Arbutin 0.1 Salicylic acid 0.2 Sodium hyaluronate 0.2 Dipropylene glycol 5.0 1,3-butylene glycol 8.0 Glycerin 3.0 Glycerin monostearate 1.0 Carboxy vinyl polymer 0.2 Xanthan gum 0.05 Meadowfoam oil 5.0 Glyceryl tri-2-ethylhexanoate 5.0 Methylpolysiloxane 1.0 Triethanolamine 0.1 Tocopherol 0.1 Acetylated sodium hyaluronate 0.05 Sodium edetate 0.05 Methyl parahydroxybenzoate 0.2 Flavoring agent 0.05 Rosemary extract 0.1 Agetsu extract 0.1 Total purified water remaining 100.0%
  • Prescription example 7 (cream) Nicotinamide 1.0 (w / w%) Glycerin 5.0 1,3-butylene glycol 5.0 Light liquid paraffin 3.0 Acrylic acid methacrylic acid alkyl copolymer 0.3 Arginine 0.3 Polyoxyethylene Cured Castor Oil 0.5 Methyl parahydroxybenzoate 0.2 Melia Azadirachta Leaf Extract 0.5 Mandarin Orange Peel Extract 3.0 Purified water total amount 100.0%
  • Prescription example 8 Tranexamic acid 1.0 (w / w%) Arbutin 3.0 Salicylic acid 0.05 Sodium hyaluronate 0.15 Dipropylene glycol 8.0 Diglycerin 5.0 Glycerin 3.0 Hydroxyethylurea 3.0 Polyoxyethylene sorbitan isostearate 0.5 Glycerin monostearate 0.4 Setostearyl Glucoside ⁇ Setostearyl Alcohol 1.5 Acrylic acid / methacrylic acid alkyl copolymer 0.5 Meadowfoam oil 0.5 Squalane 3.0 Tri (Caprylic / Capric) Glyceryl 1.5 Methylpolysiloxane 1.0 Behenyl alcohol 0.2 Stearyl alcohol 0.3 Xanthan gum 0.2 Sodium hydroxide 0.1 Sodium edetate 0.1 Methyl parahydroxybenzoate 0.2 Flavoring agent 0.1 Ceramide 1 0.01 Ceramide 2 0.01 Ceramide 6 0.01 Rosemary extract 0.001 Yukinoshita extract 0.
  • Prescription example 9 (cosmetic fluid) Nicotinamide 5.0 (w / w%) Glycerin 2.0 1,3-butylene glycol 3.0 Squalane 2.0 Triethylhexanoin 1.0 Carboxy vinyl polymer 0.2 (Acryloyl dimethyl taurine ammonium / vinyl pyrrolidone) Copolymer 0.2 Polyoxyethylene polyoxypropylene butyl ether (17E.O.) (17P.O.) 0.5 Polyoxypropylene methyl glucoside 0.5 Triethanolamine 0.15 Hydrogenated soybean phospholipids 0.5 Methyl parahydroxybenzoate 0.2 Bilberry leaf extract 0.05 Comfrey leaf extract 0.05 Purified water total amount 100.0%
  • Prescription example 10 (cosmetic fluid) Nicotinamide 5.0 (w / w%) Dipropylene glycol 5.0 1,3-butylene glycol 5.0 Light liquid paraffin 2.0 Methyl phenyl polysiloxane 1.0 Xanthan gum 0.5 (PEG-240 / decyltetradeces-20 / HDI) copolymer 1.5 Cholesteryl oleate 0.2 Polyoxyethylene Cured Castor Oil 0.5 Hydrogenated soybean phospholipids 0.2 Pentanediol 1.0 Phenoxyethanol 0.2 Macadamia nut fatty acid phytosteryl 0.1 Himefuuro extract 0.05 Fern root extract 0.05 Purified water total amount 100.0%
  • Prescription example 11 (cream) Nicotinamide 5.0 (w / w%) Glycerin 5.0 1,3-butylene glycol 10.0 1,2-pentanediol 0.5 Botanical squalane 5.0 Tri (Capryl capric acid) Glyceryl 1.0 Methylpolysiloxane 1.0 Carboxy vinyl polymer 0.4 Xanthan gum 0.05 Polyoxyethylene sorbitan monostearate 1.5 Sorbitan monostearate 0.5 Polyoxyethylene cetyl ether 0.2 Glycerin mono 2-ethylhexyl ether 0.5 Ceramide 2 0.0005 Ceramide 6 II 0.00005 Seaweed extract (1) 2.0% Altea extract 0.01 pH adjuster appropriate amount purified water total amount 100.0%
  • Prescription example 12 Ascorbyl tetra 2-hexyldecanoate 0.1 (w / w%) Trimethylglycine 1.0 2-Methylhexyl paramethoxycinnamate 10.0 Decamethylcyclopentasiloxane 20.0 Octyl palmitate 10.0 Polyoxyethylene / methylpolysiloxane copolymer 3.0 Methylhydrogenpolysiloxane treated low temperature calcined zinc oxide 15.0 Polyalkyl acrylate 5.0 Glycerin 5.0 Absolute ethanol 5.0 1,3-butylene glycol 3.0 Panthenol 0.1 Flavoring agent 0.1 Ceramide 2 0.001 Ceramide 3 0.001 Ceramide 6 0.001 Plantain Seed Extract 0.01 Beak Passion Fruit Extract 0.1 Total purified water remaining 100.0%
  • Prescription example 13 (cleansing) L-ascorbic acid 2-glucoside 0.1 (w / w%) PEG-20 Glyceryl Triisostearate 12.0 Polyglyceryl isostearate-10 8.0 Ethylhexyl palmitate 30.0 Triethyl hexanoin 20.0 Mineral oil 25.0 Diphenylsiloxyphenyl trimethicone 1.0 Hop flower extract 0.0001 Total purified water remaining 100.0%
  • Prescription example 14 (cleansing) L-ascorbic acid 2-glucoside 0.1 (w / w%) PEG-20 Glyceryl Triisostearate 12.0 Polyglyceryl isostearate-10 8.0 Ethylhexyl palmitate 28.0 Triethyl hexanoin 20.0 Mineral oil 20.0 Diphenylsiloxyphenyl trimethicone 1.0 Seaweed extract 0.0001 Altea extract 0.0001 Total purified water remaining 100.0%
  • Formulation example 15 (soft capsule: food composition)
  • the unit of numerical values in the following formulation examples of food compositions is "parts by weight”.
  • Capsule Contents Evening Primrose Oil 120 Hydrolyzable tannin 20 Hydrolyzed soy protein 20 Lotus germ extract 5 Ceramide 3 Hyaluronic acid 0.5 CoQ10 2 Hematococcus alga pigment extract (containing astaxanthin) 4 Vitamin C 1 Glycerin fatty acid ester 10
  • Bee wax 5 Capsule film Gelatin 92 Pull Run 3 Glycerin 35 Colorant 3
  • Prescription example 16 (cosmetic drink) unit (mg) Fern extract 180 Placenta extract 180 Collagen peptide 1000 Artichoke extract 50 Proteoglycan 20 Horse mackerel extract 20 Polysorbate 80 0.1 Erythritol 3 Agar 0.5 Water candy 0.6 Fructose 2 Acidulant 0.5 Flavoring agent 0.1 Water 86.1 Total daily intake (mg) 1542.9
  • Prescription example 17 (cosmetic drink) unit (mg) Seaweed extract 180 Placenta extract 180 Collagen peptide 1000 Proteoglycan 20 Rosemary extract 40 Polysorbate 80 0.1 Erythritol 3 Agar 0.5 Water candy 0.6 Fructose 2 Acidulant 0.5 Flavoring agent 0.1 Water 103.2 Daily intake total (mg) 1530.0

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Abstract

The present invention addresses the problem of providing: a novel cosmetic composition which exhibits a sufficient skin condition improvement effect that is at least comparable to intense pulse light (IPL) irradiation, with respect to skin whitening, anti-aging, and the like, and which is easy to use for many users; and a novel cosmetic method which uses same. This cosmetic composition includes an agent which exhibits IPL irradiation-like action. Furthermore, this cosmetic composition exhibits an effect comparable to light treatment, and can be used as an alternative to IPL irradiation. Moreover, it is preferable that the agent exhibiting IPL irradiation-like action in this cosmetic composition include a plant extract including flavonoids, and/or a seaweed extract.

Description

美容組成物、スクリーニング方法及び美容方法Beauty composition, screening method and beauty method
　本発明は、美容組成物、スクリーニング方法、美容方法及びＩＰＬ照射様作用剤に関する。 The present invention relates to cosmetic compositions, screening methods, cosmetic methods and IPL radiation-like agents.
　近年美容医療の分野において、光施術の人気が高まっている。中でも機器名「フォトフェイシャル（登録商標）」「ＬｉｍｅＬｉｇｈｔ」「Ｌｕｍｅｎｉｓ　Ｏｎｅ」等で知られるＩＰＬ（Ｉｎｔｅｎｓｅ　Ｐｕｌｓｅ　Ｌｉｇｈｔ）という、レーザー等とは異なる特殊な波長の光を全顔に照射することで、シミ、そばかす、小じわ、ニキビ跡、赤み等を効果的に改善することができる新しい治療法が注目されている（特許文献１、非特許文献１参照）。ＩＰＬとは、紫外線等の皮膚に有害な短波長をカットした幅広い波長域の光をいう。具体的には、可視光線及び赤外線を広く含み、５００ｎｍ～１２００ｎｍの波長域の複数の波長の光である。このように複数の波長の光を照射することにより、様々な症状を効果的に改善することができる。これらの波長域の光を、用途に応じてフィルターを用いることで波長域及び波形を限定し、皮膚症状に合わせた照射を行うことができる。この治療法は、ハリの低下や毛穴の開き、肌の色ムラ、くすみなどにも効果があり、全顔のエージング症状の改善にも有効であると言われている。上記ＩＰＬには複数の波長の光が含まれているため、従来のレーザー治療のようにシミやホクロだけといった局所的な照射ではなく、顔全体に照射していくことで、上記のような複数の問題を効果的に改善することができると考えられている。 In recent years, in the field of cosmetic medicine, light treatment has become popular. Among them, IPL (Intense Pulse Light) known by the device name "Photofacial (registered trademark)" "LimeLight" "Lumenis One" etc., stains, by irradiating all faces with light of a special wavelength different from lasers etc. New treatments that can effectively improve freckles, fine lines, acne scars, redness and the like are attracting attention (see Patent Document 1 and Non-patent Document 1). IPL refers to light in a wide wavelength range that is cut short wavelength harmful to the skin such as ultraviolet light. Specifically, it is light of a plurality of wavelengths in a wavelength range of 500 nm to 1200 nm, which widely includes visible light and infrared light. By irradiating light of a plurality of wavelengths in this manner, various symptoms can be effectively improved. By using a filter according to the application, light in these wavelength ranges can be used to limit the wavelength range and waveform, and irradiation can be performed according to skin conditions. It is said that this treatment is effective for the reduction of firmness, the opening of pores, the color unevenness of the skin, the dullness and the like, and is also effective for the alleviation of the aging symptoms of the entire face. Since the IPL contains light of a plurality of wavelengths, it is possible to irradiate the whole face with multiple irradiations as described above, instead of local irradiation such as only spot and hokuro as in conventional laser treatment. It is believed that the problem can be effectively improved.
　しかし、上記ＩＰＬ照射等の光施術を受けることができる医療機関は限られていること、治療する皮膚症状に依っては連続又は継続した施術が推奨されること、また施術に適さない肌状態又は肌特性にある人も存在することから、誰もが手軽に受けられるという訳ではない。しかしながら、肌の美白、アンチエージング等に対する要求は日々高まっているのが現状であり、それらに対し個々のライフスタイルや肌状態又は肌特性等に合わせた選択肢の提案も求められている。また、光施術は、効果は高いものの、その作用メカニズムは不明な点が多かった。 However, the medical institutions that can receive light treatment such as the above IPL radiation are limited, continuous or continuous treatment is recommended depending on the skin condition to be treated, and skin condition or not suitable for treatment Because there are people with skin characteristics, it is not that everyone can easily receive it. However, the current situation is that the demand for skin whitening, anti-aging, etc. is increasing day by day, and there is also a demand for proposals for options tailored to individual lifestyles, skin conditions, skin characteristics, etc. In addition, although light treatment is effective, its mechanism of action remains unclear.
特開２００８－１１９９４号公報JP 2008-11994 A
　本発明は、かかる状況に鑑みてなされたものであり、光施術の作用機序を明らかにし、肌の美白、アンチエージング等に関して、ＩＰＬ照射と同様に、皮膚状態の向上効果を十分に奏し、かつ多くの利用者に手軽に使用してもらえる、新しい発想の美容組成物、それを用いた新しい美容方法を提供することを課題とする。 The present invention has been made in view of such a situation, and it elucidates the mechanism of action of light treatment and sufficiently exerts a skin condition improving effect on skin whitening, anti-aging, etc., as with IPL irradiation. And, it is an object of the present invention to provide a new idea cosmetic composition which can be easily used by many users, and a new cosmetic method using it.
　本発明者らは、上記の課題を解決するために鋭意研究した結果、表皮細胞にＩＰＬ照射をすることによりメラニン合成及びメラノソーム輸送に関わる遺伝子の発現が抑制されること並びにターンオーバーに関わる遺伝子の発現が促進されることを見出した。すなわち、ＩＰＬ照射による美白メカニズムは、上記メラニン合成、メラノソーム輸送並びにターンオーバーに関わる遺伝子に働きかけることであると考えられ、この知見を利用することで、化粧品等の外用組成物でＩＰＬ照射と同様の効果を実現できる製品、すなわちＩＰＬ発想の製品を開発できることに思い至った。この知見に基づいて、ＩＰＬ照射によって発現が抑制されるこれらの遺伝子の発現を、ＩＰＬ照射と同様に抑制できる物質を探索したところ、多くの物質のうち、フラボノイドを含有する植物抽出物及び海藻抽出物がＩＰＬ照射様の効果を奏することを見出した。本発明の要旨は以下のとおりである。 As a result of intensive studies to solve the above problems, the inventors of the present invention have found that IPL irradiation to epidermal cells suppresses the expression of genes involved in melanin synthesis and melanosome transport, and genes involved in turnover. It was found that expression was promoted. That is, it is considered that the whitening mechanism by IPL irradiation is to act on the above-mentioned genes involved in melanin synthesis, melanosome transport and turnover, and by using this finding, the composition for external use such as cosmetics is similar to IPL irradiation. We came up with the idea of being able to develop products that can realize the effects, that is, products of the IPL concept. Based on this finding, we searched for substances that can suppress the expression of these genes whose expression is suppressed by IPL irradiation in the same way as IPL irradiation, and among many substances, extracts of plants containing flavonoids and extraction of seaweed It has been found that the substance exerts an IPL-like effect. The gist of the present invention is as follows.
［１］ＩＰＬ照射様作用剤を含有する、美容組成物。
［２］上記ＩＰＬ照射様作用剤が、有効成分として、フラボノイドを含有する植物抽出物を少なくとも含む、［１］に記載の美容組成物。
［３］上記植物抽出物が、ウリ科、キク科、マメ科、バラ科、ヤナギ科、センダン科、ミカン科、ユキノシタ科、オオバコ科、トケイソウ科、アサ科、モニミア科、トチノキ科、ツツジ科、ボタン科、シソ科、アオイ科、ショウガ科から成る群より選択される少なくとも一種の植物の抽出物である、［２］に記載の美容組成物。
［４］上記ＩＰＬ照射様作用剤が、有効成分として、海藻抽出物を少なくとも含む、［１］に記載の美容組成物。
［５］上記ＩＰＬ照射様作用剤が、皮膚細胞におけるメラニン関連分子の発現を調節する、［１］から［４］のいずれかに記載の美容組成物。
［６］上記メラニン関連分子が、ＯＣＡ２、ＳＬＣ４５Ａ２、及びＲＡＢ２７Ａ２から成る群より選択される少なくとも一種である、［５］に記載の美容組成物。
［７］前記植物抽出物が、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、ローズマリーエキス、ゴボウエキス、及びアルテアエキスから成る群より選択される少なくとも一種である、［２］、［３］、［５］、［６］のいずれかに記載の美容組成物。
［８］更に、ターンオーバー促進剤（上記ＩＰＬ照射様作用剤を除く）、抗糖化成分及び美白有効成分からなる群より選択される少なくとも１種を含有する、［１］から［７］のいずれかに記載の美容組成物。
［９］スキントーン向上剤用である、［１］から［８］のいずかに記載の美容組成物。
［１０］皮膚細胞にＩＰＬ照射することで発現が変化する遺伝子を指標として、スキントーン向上に効果的な成分をスクリーニングすることを特徴とする、スキントーン向上用成分のスクリーニング方法。
［１１］皮膚培養細胞に被験物質を作用させる工程、及び上記皮膚培養細胞における上記遺伝子発現を測定する工程を少なくとも含む、［１０］に記載のスクリーニング方法。
［１２］ＩＰＬ照射様作用剤を使用することを特徴とする、皮膚状態を改善・向上させるための美容方法。
［１３］アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、ローズマリーエキス、ゴボウエキス、及びアルテアエキスから成る群より選択される少なくとも一種の植物抽出物を有効成分として含む、ＩＰＬ照射様作用剤。
［１４］褐藻、紅藻、緑藻、藍藻から成る群より選択される少なくとも一種の海藻抽出物を有効成分として含む、ＩＰＬ照射様作用剤。 [1] A cosmetic composition containing an IPL radiation-like agent.
[2] The cosmetic composition according to [1], wherein the IPL irradiation-like agent at least contains a plant extract containing flavonoid as an active ingredient.
[3] The above plant extract is Cucurbitaceae, Asteraceae, Leguminosae, Rosaceae, Salixaceae, Salixaceae, Fernaceae, Citrus Department, Citrusaceae, Anchovyaceae, Anchovyaceae, Acacia, Asperiaceae, Monimimiaceae, Aescariaceae, The cosmetic composition according to [2], which is an extract of at least one kind of plant selected from the group consisting of a family of plants belonging to the family Laminaceae, the family Laminaceae, a family of mallows and a family of ginger.
[4] The cosmetic composition according to [1], wherein the IPL irradiation-like agent contains at least a seaweed extract as an active ingredient.
[5] The cosmetic composition according to any one of [1] to [4], wherein the IPL radiation-like agent modulates the expression of a melanin-related molecule in skin cells.
[6] The cosmetic composition according to [5], wherein the melanin related molecule is at least one selected from the group consisting of OCA2, SLC45A2 and RAB27A2.
[7] The plant extract is an eggplant extract, an artichoke extract, a licorice root extract, a bilberry fruit extract, a black rosewood bark extract, a meria azalea lacta leaf extract, a mandarin orange peel extract, a yukinoshita extract, a horse mackerel seed extract, a borage seed extract At least one member selected from the group consisting of fruit extract, hop flower extract, Tambrissa tricophila leaf extract, rosemary extract, burdock extract, and altea extract, [2], [3], [5], [6] The cosmetic composition according to any one of the above.
[8] Further, any one of [1] to [7], which contains at least one member selected from the group consisting of a turnover accelerator (excluding the above-mentioned IPL irradiation-like active agent), an anti-glycation component and a whitening active component A cosmetic composition according to any one of the preceding claims.
[9] The cosmetic composition according to any one of [1] to [8], which is for skin tone improver.
[10] A screening method of a component for improving skin tone, comprising screening a component effective for improving skin tone by using a gene whose expression is changed by irradiating IPL to skin cells as an index.
[11] The screening method according to [10], which comprises at least the steps of allowing a test substance to act on skin culture cells, and measuring the gene expression in the skin culture cells.
[12] A cosmetic method for improving or improving the skin condition, which comprises using an IPL irradiation-like agent.
[13] Amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, albino bark extract, Meria azalaclica leaf extract, mandarin orange peel extract, aubergine extract, horse mackerel seed extract, abalone extract fruit extract, hop flower extract An IPL radiation-like active agent comprising, as an active ingredient, at least one plant extract selected from the group consisting of Tambrissa tricophila leaf extract, rosemary extract, burdock extract, and altea extract.
[14] An IPL irradiation-like agent comprising, as an active ingredient, at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae and blue algae.
　本発明によれば、肌の美白、アンチエージング等に関して、ＩＰＬ照射と同様の皮膚状態の向上効果を十分に奏し、かつ多くの利用者に手軽に使用してもらえる、新しい発想の美容組成物を提供することができる。また、それを用いた新しい美容方法も提供することができる。 According to the present invention, a cosmetic composition of a novel idea that sufficiently achieves the same skin condition improving effect as IPL irradiation with respect to skin whitening, anti-aging, etc. and can be easily used by many users. Can be provided. In addition, new cosmetic methods using it can also be provided.
　以下、本発明について詳細に説明する。なお、本明細書中で使用される用語は、特に言及しない限り、当該技術分野で通常用いられる意味で解釈される。 Hereinafter, the present invention will be described in detail. The terms used in the present specification are interpreted in the meaning commonly used in the art unless otherwise stated.
＜美容組成物＞
　本発明の美容組成物は、ＩＰＬ照射様作用剤を含有する。ここで、ＩＰＬ照射様作用剤とは、被験体に対してＩＰＬを照射したときと同様の効果を与えることが可能な組成物をいう。これはＩＰＬ照射等の光施術の代替となり得る組成物、すなわちＩＰＬ照射代替用美容組成物である。なお、ＩＰＬ照射様作用剤としては、ＩＰＬを被験体に照射して得られる効果のうちの一部の効果を与えるものであってもよい。本発明の美容組成物は、単独でも効果を奏するものであるが、ＩＰＬ照射等の光施術と組み合わせることにより、ＩＰＬ照射の効果を向上させ、長時間持続させることも可能である。 <Beauty composition>
The cosmetic composition of the present invention contains an IPL radiation-like agent. Here, the IPL irradiation-like agent refers to a composition capable of giving the same effect as when the subject is irradiated with IPL. This is a composition that can replace light treatment such as IPL radiation, ie a cosmetic composition for IPL radiation substitution. In addition, as an IPL irradiation-like active agent, some effects may be given among the effects obtained by irradiating IPL to a subject. The cosmetic composition of the present invention is effective even if it is used alone, but it is also possible to improve the effect of IPL irradiation and maintain it for a long time by combining it with the light treatment such as IPL irradiation.
　ＩＰＬを被験体に照射して得られる効果としては、例えば、皮膚細胞におけるメラニン関連分子の発現調節効果、全顔の美白、スキントーンの向上効果が得られる。また、全顔のくすみ、ハリのなさ、毛穴目立ち、肌の色むら、乾燥などのエージング症状の改善効果（スキントーンの改善効果）が挙げられる。上記メラニン関連分子（遺伝子及び／又はタンパク）としては、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ２、ＨＢ‐ＥＧＦ等が挙げられる。ＩＰＬ照射によって、これらの分子のうち、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ２の発現は抑制する方向に調節される。また、これらの分子のうち、ＨＢ－ＥＧＦの発現は促進する方向に調節される。すなわち、本発明の美容組成物は、ＯＣＡ２、ＳＬＣ４５Ａ２及びＲＡＢ２７Ａ２から成る群より選択される少なくとも一種の遺伝子発現抑制用の美容組成物、又はＨＢ－ＥＧＦの遺伝子発現促進用の美容組成物であるとも言える。また、本発明の美容組成物は、上記の遺伝子発現を抑制又は促進することから、ＩＰＬ照射と同様のスキントーン向上用及び／又はスキントーン改善用の美容組成物であるとも言える。 The effects obtained by irradiating the subject with IPL include, for example, the expression regulation effect of melanin-related molecules in skin cells, the skin whitening effect of all faces, and the improvement effect of skin tone. In addition, there are improvement effects (improvement effect of skin tone) of aging symptoms such as dullness of the whole face, no firmness, pore appearance, uneven skin color, dryness and the like. Examples of the melanin related molecules (genes and / or proteins) include OCA2, SLC45A2, RAB27A2, HB-EGF and the like. Among these molecules, expression of OCA2, SLC45A2 and RAB27A2 is regulated in a repressing direction by IPL irradiation. Also, among these molecules, expression of HB-EGF is regulated in a promoting direction. That is, the cosmetic composition of the present invention may be a cosmetic composition for suppressing gene expression of at least one selected from the group consisting of OCA2, SLC45A2 and RAB27A2, or a cosmetic composition for promoting gene expression of HB-EGF. I can say that. Further, the cosmetic composition of the present invention can be said to be a cosmetic composition for skin tone improvement and / or skin tone improvement similar to IPL irradiation because it suppresses or promotes the above-mentioned gene expression.
　本発明の美容組成物は、上記ＩＰＬ照射様作用剤に加えて、本発明の効果を損なわない範囲で、その他の任意成分を含んでいてもよい。以下に、ＩＰＬ照射様作用剤及びその他の任意成分について説明する。 The cosmetic composition of the present invention may contain other optional components in addition to the above IPL radiation-like active agent as long as the effects of the present invention are not impaired. The following describes the IPL radiation-like agent and other optional components.
　［ＩＰＬ照射様作用剤］
　本発明の美容組成物においてＩＰＬ照射様作用剤は、被験体にＩＰＬ照射をしたときと同様の効果を与えることが可能な物質であれば特に限定されないが、例えば、各種植物抽出物等を有効成分として含有するものが挙げられ、中でもフラボノイドを含有する植物抽出物を少なくとも含むものであることが好ましい。また、海藻抽出物を少なくとも含むものであることも好ましい。 [IPL irradiation like agent]
In the cosmetic composition of the present invention, the IPL irradiation-like agent is not particularly limited as long as it is a substance that can give the same effect as when IPL irradiation is given to a subject, but, for example, various plant extracts etc. are effective What is contained as a component is mentioned, and it is preferable that it is a thing which at least contains the plant extract containing flavonoid. Moreover, it is also preferable that it is a thing containing a seaweed extract at least.
　フラボノイドを含有する植物抽出物としては、例えば、ウリ科、キク科、マメ科、バラ科、ヤナギ科、センダン科、ミカン科、ユキノシタ科、オオバコ科、トケイソウ科、アサ科、モニミア科、トチノキ科、ツツジ科、ボタン科、シソ科、アオイ科、ショウガ科等の植物の抽出物が挙げられる。 Examples of plant extracts containing flavonoids include Cucurbitaceae, Asteraceae, Leguminosae, Rosaceae, Salixaceae, Salixaceae, Neridaceae, Citrusaceae, Citrusaceae, Pisidiomycidae, Salamanders, Asinaceae, Monimimiaceae, Aquilinaceae Extracts of plants such as azaleas, botans, mints, mallows, gingers and the like can be mentioned.
　上記植物抽出物としては、例えば、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、フユボダイジュ花エキス、加水分解ダイズペプチド、セイヨウシロヤナギ樹皮エキス、イザヨイバラエキス、ローズマリーエキス、ゴボウエキス、アルテアエキス等が挙げられる。上記植物の抽出する部位としては、例えば葉、花、実、根、茎、皮、枝、果実、果皮、種子などが挙げられる。抽出に際しては植物原料をそのまま用いてもよく、乾燥させて用いてもよい。植物原料は粉砕して用いることもできる。また、化粧品原料等として市販されている植物エキスを使用してもよい。 As the above-mentioned plant extract, for example, amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, white syllable bark extract, melia azalea lacta leaf extract, mandarin orange peel extract, yukinoshita extract, horse mackerel seed extract, borage Examples thereof include fruit extract, hop flower extract, Tambrissa tricophila leaf extract, Hybodaidai flower extract, hydrolysed soybean peptide, white willow bark extract, lice tree extract, rosemary extract, burdock extract, altea extract and the like. As a site which the above-mentioned plant extracts, a leaf, a flower, a fruit, a root, a stem, a skin, a branch, a fruit, a peel, a seed etc. are mentioned, for example. At the time of extraction, a plant material may be used as it is or may be dried and used. Plant materials can also be used after being crushed. Moreover, you may use the plant extract marketed as a cosmetics raw material etc.
　上記海藻抽出物に用いられる海藻としては、例えば、褐藻（ガゴメコンブ、リシリコンブ、ミツイシコンブ、マコンブ、ナガコンブ、ワカメ等）、紅藻（テングサ等）、緑藻（アオノリ等）、藍藻（スピルリナ等）等の海藻類の抽出物が挙げられる。抽出に際しては、海藻原料をそのまま用いてもよく、乾燥させて用いても良い。植物原料は粉砕して用いることもできる。また、化粧品原料等として市販されている海藻エキスを使用しても良い。これら海藻類は１種単独で用いても良いし、２種以上を併用しても良い。これらのうち、メラニン関連分子の発現を促進させる効果に優れるという観点から、褐藻の抽出物が好ましく、コンブ抽出物がより好ましく、ガゴメコンブ抽出物がさらに好ましい。 Examples of seaweeds used for the above seaweed extract include seaweeds such as brown algae (Gagome kombu, Risei ibu, mitsuishi kombu, macombe, naga kombu, wakame etc.), red algae (eg Tengusa etc.), green algae (Aonori etc.), blue-green algae (spyrulina etc.) And the like extracts. In the extraction, the seaweed raw material may be used as it is or may be dried and used. Plant materials can also be used after being crushed. Moreover, you may use the seaweed extract marketed as a cosmetics raw material etc. These seaweeds may be used alone or in combination of two or more. Among these, from the viewpoint of being excellent in the effect of promoting the expression of melanin-related molecules, extracts of brown algae are preferable, extracts of kelp are more preferable, and extracts of gagome comb are more preferable.
　これらのうち、メラニン関連分子の中で、特にＯＣＡ２遺伝子の発現を低下させる効果に優れるという観点から、アーティチョークエキス、クダモノトケイソウ果実エキス、アマチャヅルエキス、加水分解ダイズペプチド（加水分解ダイズタンパク）、カンゾウ根エキス、ノイバラ果実エキス、タンブリッサトリコフィラ葉エキス、フユボダイジュ花エキスが好ましく、アーティチョークエキス、クダモノトケイソウ果実エキス、カンゾウ根エキス、ノイバラ果実エキスがより好ましい。 Among these, among the melanin-related molecules, from the viewpoint of being excellent in the effect of reducing the expression of the OCA2 gene, particularly, artichoke extract, scutellaria root fruit extract, amacha mulberry extract, hydrolyzed soybean peptide (hydrolyzed soybean protein), licorice root The extract, the neubara fruit extract, the tambrissa tricophila leaf extract, and the pufferfish flower extract are preferable, and the artichoke extract, the bilberry fruit juice extract, the licorice root extract, and the neurala fruit extract are more preferable.
　メラニン関連分子の中で、特にＳＬＣ４５Ａ２遺伝子の発現を低下させる効果に優れるという観点から、アマチャヅルエキス、カンゾウ根エキス、ノイバラ果実エキス、アーティチョークエキス、加水分解ダイズペプチド、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、フユボダイジュ花エキスが好ましく、アマチャヅルエキス、カンゾウ根エキス、ノイバラ果実エキスがより好ましい。 Among melanin-related molecules, particularly from the viewpoint of being excellent in reducing the expression of the SLC45A2 genera extract, licorice root extract, Neubara fruit extract, artichoke extract, hydrolyzed soybean peptide, white willow bark extract, Melia Azadirachta Leaf extract, mandarin orange peel extract, Yukinoshita extract, horse mackerel seed extract, and flower extract are preferable, and amacha mulberry extract, licorice root extract and neubara fruit extract are more preferable.
　メラニン関連分子の中で、特にＲＡＢ２７Ａ２遺伝子の発現を低下させる効果に優れるという観点から、アマチャヅルエキス、アーティチョークエキス、加水分解ダイズペプチド、カンゾウ根エキス、ノイバラ果実エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、タンブリッサトリコフィラ葉エキス、フユボダイジュ花エキス、イザヨイバラエキスが好ましい。 Among melanin-related molecules, particularly from the viewpoint of being excellent in the effect of reducing the expression of RAB27A2, amachar extract, artichoke extract, hydrolyzed soy peptide, licorice root extract, Neubara fruit extract, Yukinoshita extract, Horse mackerel seed extract, tan Preferred are Brissatricophila leaf extract, Fubodaiji flower extract and Izayoibara extract.
　メラニン関連分子のうち、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ２のいずれか２つ以上の遺伝子発現を低下させる効果に優れるという観点から、アマチャヅルエキス、アーティチョークエキス、加水分解ダイズペプチド、カンゾウ根エキス、ノイバラ果実エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、フユボダイジュ花エキス、セイヨウシロヤナギ樹皮エキス、マンダリンオレンジ果皮エキス、タンブリッサトリコフィラ葉エキスが好ましく、３つの遺伝子全ての発現を低下させる効果に優れるという観点から、アマチャヅルエキス、アーティチョークエキス、加水分解ダイズペプチド、カンゾウ根エキス、ノイバラ果実エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、フユボダイジュ花エキスがより好ましい。 Among the melanin-related molecules, from the viewpoint of the excellent effect of reducing the gene expression of any two or more of OCA2, SLC45A2 and RAB27A2, amacha mulle extract, artichoke extract, hydrolyzed soy peptide, licorice root extract, Neubara fruit extract, Yukinoshita Extract, horseradish seed extract, pufferfish flower extract, white syllable bark extract, mandarin orange peel extract, and Tamburissa Trichophila leaf extract are preferable, and from the viewpoint of being excellent in the effect of reducing the expression of all three genes, Artichoke extract, hydrolyzed soybean peptide, licorice root extract, Neubara fruit extract, Yukinoshita extract, Horse mackerel seed extract, Hybodaige flower extract is more preferable
　メラニン関連分子のうち、ＨＢ‐ＥＧＦ遺伝子の発現を促進させる効果に優れるという観点から、ローズマリーエキス、海藻抽出物、アルテアエキス、クダモノトケイソウ果実エキス、ゴボウエキスが好ましく、海藻抽出物、アルテアエキス、ローズマリーエキスがより好ましい。 Among the melanin-related molecules, from the viewpoint of being excellent in the effect of promoting the expression of the HB-EGF gene, rosemary extract, seaweed extract, altea extract, albino fruit extract and burdock extract are preferable, seaweed extract, altea extract, Rosemary extract is more preferred.
　本発明の効果を総合的に鑑みると、本発明の美容組成物における上記植物抽出物としては、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、ローズマリーエキス、アルテアエキスが好ましい。また、本発明の美容組成物における上記海藻抽出物としては褐藻抽出物が好ましく、コンブ抽出物がより好ましく、ガゴメコンブ抽出物がさらに好ましい。 In view of the effects of the present invention comprehensively, as the above-mentioned plant extract in the cosmetic composition of the present invention, amacha spruce extract, artichoke extract, licorice root extract, neubara fruit extract, black radish bark extract, melia azalacilla leaf extract Among them, mandarin orange peel extract, Yukinoshita extract, horseradish seed extract, scutellaria root fruit extract, hop flower extract, Tambrissa tricophila leaf extract, rosemary extract and altea extract are preferable. Moreover, as said seaweed extract in the cosmetics composition of this invention, a brown-alga extract is preferable, a kelp extract is more preferable, and a gagome kombu extract is further more preferable.
　本発明の美容組成物における上記ＩＰＬ照射様作用剤の含有量としては、上記ＩＰＬ照射様作用剤が上記植物抽出物である場合、０．０００１重量％～２０重量％であり、０．００１重量％～１０重量％であることが好ましく、０．０１重量％～５重量％であることがより好ましく、０．０５重量％～３重量％であることがさらに好ましい。なお、本発明の美容組成物は、上記植物抽出物を１種単独で含んでもよいし、２種以上を含んでもよい。本発明の美容組成物が上記植物抽出物を２種以上含む場合、これら２種以上の植物抽出物の含有量の合計を上記植物抽出物の含有量とする。 The content of the IPL irradiation-like agent in the cosmetic composition of the present invention is 0.0001% to 20% by weight when the IPL irradiation-like agent is the plant extract, 0.001 weight % To 10% by weight is preferable, 0.01% to 5% by weight is more preferable, and 0.05% to 3% by weight is further preferable. The cosmetic composition of the present invention may contain the above-mentioned plant extract singly or in combination of two or more. When the cosmetic composition of the present invention contains two or more plant extracts, the total content of the two or more plant extracts is taken as the content of the plant extract.
　本発明による美容方法、美容組成物は、チロシナーゼ活性阻害等による従来の美白メカニズムとは異なり、肌の白さを決定づけるメラニン合成及びメラノソーム輸送に関わる遺伝子に直接働きかけることで、スキントーンをアップさせる効果（スキントーン向上効果）を奏するものである。 The cosmetic method according to the present invention, the cosmetic composition, unlike the conventional whitening mechanism by tyrosinase activity inhibition etc., has an effect of increasing skin tone by directly acting on genes involved in melanin synthesis and melanosome transport that determine whiteness of the skin. (Skin tone improvement effect).
　ここで、スキントーンのアップとは、シミやソバカス、加齢に伴う肌のくすみや色素沈着を改善し、より自然で均一な肌色に近づけること（スキントーン改善）に加えて、本来よりの肌色のトーンが濃い肌の肌トーンをアップさせることも含まれる。 Here, with skin tone up, it improves stains, buckwheat, skin dullness and pigmentation associated with aging, and brings it closer to a more natural and even skin color (skin tone improvement) as well as the original skin color. It also includes raising the skin tone of dark skin.
　本発明の美容組成物が含む任意成分としては、例えば、美白有効成分、ターンオーバー促進剤（本発明のＩＰＬ照射様作用剤を除く）、抗糖化成分、抗酸化成分、老化防止成分、抗炎症剤、清涼化剤、殺菌剤、ビタミン類、有機酸、保湿成分、多価アルコール、スクラブ剤、紫外線吸収成分、紫外線散乱成分、収斂成分、ペプチド又はその誘導体、アミノ酸又はその誘導体、洗浄成分、角質柔軟成分、細胞賦活化成分、血行促進作用成分等が挙げられる。なお、本発明の美容組成物において、これらの成分は、それぞれ１種単独で用いてもよいし、２種以上を併用してもよい。 As optional components contained in the cosmetic composition of the present invention, for example, a whitening active component, a turnover accelerator (except for the IPL irradiation-like active agent of the present invention), an antiglycation component, an antioxidant component, an antiaging component, an antiinflammatory agent Agent, refreshing agent, disinfectant, vitamins, organic acid, moisturizing component, polyhydric alcohol, scrub agent, ultraviolet absorbing component, ultraviolet scattering component, astringent component, peptide or derivative thereof, amino acid or derivative thereof, cleansing component, keratin Soft components, cell activation components, circulation promoting action components and the like can be mentioned. In the cosmetic composition of the present invention, these components may be used alone or in combination of two or more.
　上記美白有効成分としては、例えば、トコフェロール、ビタミンＣ及びその誘導体、アルブチン、コウジ酸、プラセンタ、エラグ酸、ニコチン酸アミド、トラネキサム酸及びその誘導体、ハイドロキノン、ルシノール（登録商標）、４－メトキシサリチル酸カリウム塩、リノール酸及びその誘導体、等が挙げられる。 Examples of the whitening active ingredients include tocopherol, vitamin C and derivatives thereof, arbutin, kojic acid, placenta, ellagic acid, nicotinamide, tranexamic acid and derivatives thereof, hydroquinone, Lucinol (registered trademark), and potassium 4-methoxysalicylic acid. Salts, linoleic acid and derivatives thereof, and the like.
　上記ターンオーバー促進剤としては、例えば、後述するビタミン類、角質柔軟成分、細胞賦活化成分、血行促進成分等が挙げられる。 Examples of the above-mentioned turnover promoting agent include vitamins to be described later, keratin softness component, cell activation component, blood circulation promoting component and the like.
　上記抗糖化成分としては、例えば、ブドレジャアキシラリス葉エキス等の植物エキス、月見草油、アムラーの果実、果汁又はそれらの抽出物、Ｌ－アルギニン、Ｌ－リジン、加水分解カゼイン、加水分解性タンニン、カルノシン等が挙げられる。 Examples of the above-mentioned anti-glycation component include plant extracts such as budgera akisilalis leaf extract, evening primrose oil, fruits of Amlar, fruit juice or extracts thereof, L-arginine, L-lysine, hydrolysed casein, hydrolyzable tannin , Carnosine and the like.
　上記抗酸化成分としては、例えば、植物（例えば、ブドウ、オタネニンジン、及びコンフリー等）に由来する成分；プロアントシアニジン、トコフェロール及びその誘導体、アスコルビン酸及びその誘導体、へスペリジン及びその誘導体、エルゴチオネイン、亜硫酸水素ナトリウム、エリソルビン酸及びその塩、フラボノイド、グルタチオン等が挙げられる。 Examples of the above antioxidant component include components derived from plants (eg, grape, ginseng, comfrey etc.); proanthocyanidin, tocopherol and derivatives thereof, ascorbic acid and derivatives thereof, hesperidin and derivatives thereof, ergothioneine, sulfite Examples include sodium hydrogen, erythorbic acid and its salts, flavonoids, glutathione and the like.
　上記老化防止成分としては、例えば、加水分解大豆タンパク、レチノイド（レチノール及びその誘導体、レチノイン酸、及びレチナール等）、パンガミン酸、カイネチン、ウルソール酸、ウコンエキス、スフィンゴシン誘導体、ケイ素、ケイ酸、Ｎ－メチル－Ｌ－セリン、メバロノラクトン等が挙げられる。 Examples of the antiaging component include hydrolyzed soybean protein, retinoid (retinol and derivatives thereof, retinoic acid, and retinal and the like), pangamic acid, kinetin, ursolic acid, turmeric extract, sphingosine derivative, silicon, silicic acid, N- Methyl-L-serine, mevalonolactone and the like can be mentioned.
　上記抗炎症剤としては、例えば、アラントイン及びその誘導体、グリチルレチン酸及びその誘導体、グリチルリチン酸及びその誘導体、サリチル酸誘導体、アミノカプロン酸、アズレン及びその誘導体、酸化亜鉛、酢酸トコフェロール等が挙げられる。 Examples of the anti-inflammatory agent include allantoin and derivatives thereof, glycyrrhetinic acid and derivatives thereof, glycyrrhizinic acid and derivatives thereof, salicylic acid derivatives, aminocaproic acid, azulene and derivatives thereof, zinc oxide, tocopherol acetate and the like.
　上記清涼化剤としては、例えば、メントール、カンフル、ゲラニオール、等のテルペン類（これらはｄ体、ｌ体又はｄｌ体のいずれでもよい。）；ユーカリ油、ベルガモット油、ペパーミント油、クールミント油、スペアミント油、ハッカ油、等の精油等が挙げられる。 As the above-mentioned refreshing agent, for example, terpenes such as menthol, camphor, geraniol and the like (these may be d-form, l-form or dl-form); Eucalyptus oil, bergamot oil, peppermint oil, coolmint oil, Examples include essential oils such as spearmint oil and peppermint oil.
　上記殺菌剤としては、例えば、イソプロピルメチルフェノール、サリチル酸、塩化ベンザルコニウム、エタノール、塩化ベンゼトニウム、グルコン酸及びその誘導体、感光素１０１号、感光素２０１号、パラベン、フェノキシエタノール、ピロクトオラミン、ミコナゾール等が挙げられる。 Examples of the germicides include isopropylmethylphenol, salicylic acid, benzalkonium chloride, ethanol, benzethonium chloride, gluconic acid and derivatives thereof, Photosensitizer No. 101, Photosensitizer No. 201, parabens, phenoxyethanol, piroctoramine, miconazole and the like. Be
　上記ビタミン類としては、水溶性ビタミン及び油溶性ビタミンのいずれであってもよく、例えば、ビタミンＢ６類、パントテン酸類、ニコチン酸類、ビタミンＢ１類、ビタミンＢ２類、ビオチン類葉酸類、ビタミンＢ１２類、水溶性のビタミンＣ類、油溶性のビタミンＣ類、ビタミンＫ類；フェルラ酸等のビタミン様作用因子等が挙げられる。 The above-mentioned vitamins may be any of water-soluble vitamins and oil-soluble vitamins, for example, vitamin B6s, pantothenic acids, nicotinic acids, vitamin B1s, vitamin B2s, biotins folates, vitamin B12s, Examples thereof include water-soluble vitamin C, oil-soluble vitamin C, vitamin K, and vitamin-like agents such as ferulic acid.
　上記有機酸としては、例えば、グルコン酸、アスパラギン酸、アミノエチルスルホン酸、クエン酸、グルタミン酸、コハク酸、シュウ酸、フマル酸、プロピオン酸、リンゴ酸、サリチル酸、グリコール酸、フィチン酸、酒石酸、酢酸、乳酸、及びこれらの塩が挙げられる。塩としては、例えば、硫酸、塩酸又はリン酸等の鉱酸の塩、マレイン酸又はメタンスルホン酸等の有機酸の塩、ナトリウム又はカリウム等のアルカリ金属塩、アルカリ土類金属塩、アンモニウム塩等が挙げられる。 Examples of the organic acids include gluconic acid, aspartic acid, aminoethylsulfonic acid, citric acid, glutamic acid, succinic acid, oxalic acid, fumaric acid, propionic acid, malic acid, salicylic acid, glycolic acid, phytic acid, tartaric acid, acetic acid , Lactic acid, and their salts. As the salt, for example, salts of mineral acids such as sulfuric acid, hydrochloric acid or phosphoric acid, salts of organic acids such as maleic acid or methanesulfonic acid, alkali metal salts such as sodium or potassium, alkaline earth metal salts, ammonium salts etc. Can be mentioned.
　上記保湿成分としては、例えば、ヒアルロン酸ナトリウム、ヘパリン類似物質、コンドロイチン硫酸ナトリウム、コラーゲン、エラスチン、ケラチン、キチン、キトサン、プロテオグリカン等の高分子化合物；グリシン、アスパラギン酸、アルギニン等のアミノ酸；乳酸ナトリウム、尿素、ピロリドンカルボン酸ナトリウム等の天然保湿因子；セラミド、コレステロール、リン脂質等の脂質；カミツレエキス、ハマメリスエキス、チャエキス、シソエキス等の植物抽出エキス、グリセリンなどの多価アルコール等が挙げられる。 Examples of the moisturizing component include high molecular weight compounds such as sodium hyaluronate, heparin analogue, sodium chondroitin sulfate, collagen, elastin, keratin, chitin, chitosan, proteoglycan and the like; amino acids such as glycine, aspartic acid and arginine; sodium lactate, Natural moisturizing factors such as urea and sodium pyrrolidonecarboxylate; lipids such as ceramide, cholesterol and phospholipids; plant extracts such as chamomile extract, hamamelis extract, tea extract and shiso extract, and polyhydric alcohols such as glycerin and the like.
　上記多価アルコールとしては、炭素数２～１０のものが好ましく、例えば、グリセリン、ジグリセリン、トリグリセリン、プロピレングリコール、ジプロピレングリコール、１，３－ブタンジオール、エチレングリコール、ジエチレングリコール、イソプレングリコール、１、３－ブチレングリコール、ソルビトール、キシリトール、エリスリトール、マンニトール、ペンタンジオール、ヘキサンジオール、オクタンジオール、デカンジオール、ネオペンチルグリコール等が挙げられる。 The polyhydric alcohol preferably has 2 to 10 carbon atoms, and examples thereof include glycerin, diglycerin, triglycerin, propylene glycol, dipropylene glycol, 1,3-butanediol, ethylene glycol, diethylene glycol, isoprene glycol, 1 And 3-butylene glycol, sorbitol, xylitol, erythritol, mannitol, pentanediol, hexanediol, octanediol, decanediol, neopentyl glycol and the like.
　上記スクラブ剤としては、例えば、アプリコット核粉末、アーモンド殻粉末、アンズ核粉末、塩化ナトリウム粒、オリーブ核粉末、海水乾燥物粒、キャンデリラワックス、くるみ殻粉末、さくらんぼ核粉末、サンゴ粉末、炭粉末、はしばみ殻粉末、ポリエチレン末、無水ケイ酸等が挙げられる。 As the above-mentioned scrub agent, for example, apricot kernel powder, almond shell powder, apricot kernel powder, sodium chloride particles, olive kernel powder, dried seawater particles, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, charcoal powder And husk powder, polyethylene powder, silicic acid anhydride and the like.
　上記紫外線吸収成分としては、例えば、メトキシケイ皮酸エチルヘキシル、ジエチルアミノヒドロキシベンゾイル安息香酸ヘキシル、ビスエチルヘキシルオキシフェノールメトキシフェニルトリアジン、エチルヘキシルトリアゾン、ｔ－ブチルメトキシジベンゾイルメタン、オクトクリレン、ポリシリコーン－１５、メチレンビスベンゾトリアゾリルテトラメチルブチルフェノール、ジメトキシベンジリデンジオキソイミダゾリジンプロピオン酸オクチル、フェニルベンズイミダゾールスルホン酸等が挙げられる。 Examples of the UV absorbing component include ethylhexyl methoxycinnamate, hexyl diethylaminohydroxybenzoylbenzoate, bisethylhexyl oxyphenol methoxyphenyl triazine, ethylhexyl triazone, t-butyl methoxy dibenzoyl methane, octocrylene, polysilicone-15, methylene Bisbenzotriazolyl tetramethylbutylphenol, dimethoxybenzylidene dioxoimidazolidine octylate, phenyl benzimidazole sulfonic acid and the like can be mentioned.
　上記紫外線散乱成分としては、例えば、含水ケイ酸、ケイ酸亜鉛、ケイ酸セリウム、ケイ酸チタン、酸化ジルコニウム、酸化セリウム、酸化チタン、酸化鉄、酸化亜鉛、無水ケイ酸等の無機化合物、これらの無機化合物を含水ケイ酸、水酸化アルミニウム、マイカやタルク等の無機粉体で被覆したり、ポリアミド、ポリエチレン、ポリエステル、ポリスチレン、ナイロン等の樹脂粉体に複合化したもの、さらにシリコーン油や脂肪酸アルミニウム塩等で処理したもの等が挙げられる。 Examples of the ultraviolet light scattering component include inorganic compounds such as hydrous silicic acid, zinc silicate, cerium silicate, titanium silicate, titanium oxide, zirconium oxide, cerium oxide, titanium oxide, iron oxide, zinc oxide, silicic anhydride, etc. An inorganic compound coated with an inorganic powder such as hydrous silicic acid, aluminum hydroxide, mica or talc, or compounded with a resin powder such as polyamide, polyethylene, polyester, polystyrene or nylon, and further silicone oil or fatty acid aluminum What was processed by salt etc. is mentioned.
　上記収斂成分としては、例えば、ミョウバン、硫酸亜鉛、塩化アルミニウム、スルホ石炭酸亜鉛、タンニン酸等が挙げられる。 Examples of the astringent component include alum, zinc sulfate, aluminum chloride, zinc sulfocarbonate, tannic acid and the like.
　上記ペプチド又はその誘導体としては、例えば、ケラチン分解ペプチド、加水分解ケラチン、コラーゲン、魚由来コラーゲン、アテロコラーゲン、ゼラチン、エラスチン、エラスチン分解ペプチド、コラーゲン分解ペプチド、加水分解コラーゲン、塩化ヒドロキシプロピルアンモニウム加水分解コラーゲン、エラスチン分解ペプチド、コンキオリン分解ペプチド、加水分解コンキオリン、シルク蛋白分解ペプチド、加水分解シルク、ラウロイル加水分解シルクナトリウム、大豆蛋白分解ペプチド、加水分解大豆蛋白、小麦蛋白、小麦蛋白分解ペプチド、加水分解小麦蛋白、カゼイン分解ペプチド、アシル化ペプチド（パルミトイルオリゴペプチド、パルミトイルペンタペプチド、パルミトイルテトラペプチド等）等が挙げられる。 Examples of the peptide or derivatives thereof include keratinolytic peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin degrading peptides, collagenolytic peptides, hydrolyzed collagen, hydroxypropyl ammonium chloride hydrolyzed collagen, Elastin-degrading peptide, Conchiolin-degrading peptide, Hydrolyzed conquioline, Silk-proteolytic peptide, Hydrolyzed silk, Lauroyl-hydrolyzed silk sodium, Soy-proteolytic peptide, Hydrolyzed soy protein, Wheat protein, Wheat-proteolytic peptide, Hydrolyzed wheat protein, Caseinolytic peptides, acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.) and the like can be mentioned.
　上記アミノ酸又はその誘導体としては、例えば、ベタイン（トリメチルグリシン）、プロリン、ヒドロキシプロリン、アルギニン、リジン、セリン、グリシン、アラニン、フェニルアラニン、β－アラニン、スレオニン、グルタミン酸、グルタミン、アスパラギン、アスパラギン酸、システイン、シスチン、メチオニン、ロイシン、イソロイシン、バリン、ヒスチジン、タウリン、γ－アミノ酪酸、γ－アミノ－β－ヒドロキシ酪酸、カルニチン、カルノシン、クレアチン等が挙げられる。 Examples of the above amino acids or derivatives thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, Cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, creatine and the like.
　上記洗浄成分としては、例えば、ラウリン酸カリウム、ミリスチン酸カリウム、パルミチン酸カリウム又はステアリン酸カリウム等のアルカリ金属塩、アルカノールアミド塩又はアミノ酸塩等から選ばれる石けん類；ココイルグルタミン酸Ｎａ、ココイルメチルタウリンＮａ等のアミノ酸系界面活性剤；ラウレス硫酸Ｎａ等のエーテル硫酸エステル塩；ラウリルエーテル酢酸Ｎａ等のエーテルカルボン酸塩；アルキルスルホコハク酸エステルＮａ等のスルホコハク酸エステル塩；ヤシ油脂肪酸モノエタノールアミド、ヤシ油脂肪酸ジエタノールアミド等の脂肪酸アルカノールアミド；ラウリルリン酸ナトリウム、ポリオキシエチレンラウリルエーテルリン酸ナトリウム等のモノアルキルリン酸エステル塩；ヤシ油脂肪酸アミドプロピルジメチルアミノ酢酸ベタイン、ラウリルジメチルアミノ酢酸ベタイン、２－アルキル－Ｎ－カルボキシメチル－Ｎ－ヒドロキシエチルイミダゾリニウムベタイン、ラウリルヒドロキシスルホベタイン及びラウロイルアミドエチルヒドロキシエチルカルボキシメチルベタインヒドロキシプロピルリン酸ナトリウム等のベタイン型両性界面活性剤；ラウリルアミノプロピオン酸ナトリウム等のアミノ酸型両性界面活性剤等が挙げられる。 Examples of the washing component include soaps selected from alkali metal salts such as potassium laurate, potassium myristate, potassium palmitate or potassium stearate, alkanolamide salts or amino acid salts, etc .; cocoylglutamate Na, cocoyl methyltaurine Na Amino acid surfactant such as ether sulfate ester salt such as sodium laureth sulfate ether carboxylate salt such as sodium lauryl ether acetate salt sulfosuccinic acid ester salt such as alkyl sulfosuccinic ester Na such as coconut oil fatty acid monoethanolamide, coconut oil Fatty acid alkanolamides such as fatty acid diethanolamide; monoalkyl phosphate ester salts such as sodium lauryl phosphate, polyoxyethylene lauryl ether sodium phosphate; coconut oil fatty acid amido propyl dime L-aminoacetic acid betaine, lauryl dimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryl hydroxysulfobetaine and lauroylamidoethyl hydroxyethyl carboxymethyl betaine sodium hydroxypropyl phosphate and other betaines Amphoteric surfactants; amino acid type amphoteric surfactants such as sodium lauryl aminopropionate and the like can be mentioned.
　上記角質柔軟成分としては、例えば、乳酸、サリチル酸、グルコール酸、クエン酸、リンゴ酸、フルーツ酸、フィチン酸、尿素、イオウ等が挙げられる。 Examples of the keratin softness component include lactic acid, salicylic acid, glycolic acid, citric acid, malic acid, fruit acid, phytic acid, urea, sulfur and the like.
　上記細胞賦活化成分としては、例えば、γ－アミノ酪酸等のアミノ酸類；レチノール、チアミン、リボフラビン、塩酸ピリドキシン、パントテン酸類等のビタミン類；グリコール酸、乳酸等のα－ヒドロキシ酸類；タンニン、フラボノイド、サポニン、感光素３０１号等が挙げられる。 Examples of the cell activation component include amino acids such as γ-aminobutyric acid; vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids; α-hydroxy acids such as glycolic acid and lactic acid; tannin, flavonoid, Saponin, photosensitive element No. 301 and the like can be mentioned.
　上記血行促進作用成分としては、植物（例えば、オタネニンジン、アシタバ、アルニカ、イチョウ、ウイキョウ、エンメイソウ、オランダカシ、カミツレ、ローマカミツレ、カロット、ゲンチアナ、ゴボウ、コメ、サンザシ、シイタケ、ショウガ、セイヨウサンザシ、セイヨウネズ、センキュウ、センブリ、タイム、チョウジ、チンピ、トウガラシ、トウキ、トウニン、トウヒ、ニンジン、ニンニク、ブッチャーブルーム、ブドウ、ボタン、マロニエ、メリッサ、ユズ、ヨクイニン、リョクチャ、ローズマリー、ローズヒップ、チンピ、トウキ、トウヒ、モモ、アンズ、クルミ、トウモロコシ等）に由来する成分；アセチルコリン、イクタモール、カンタリスチンキ、ガンマーオリザノール、セファランチン、トラゾリン、ニコチン酸トコフェロール、グルコシルヘスペリジン等が挙げられる。 Examples of the above-mentioned blood circulation promoting active ingredient include plants (eg, Panax ginseng, hornhead, Arnica, Ginkgo biloba, Ginkgo biloba, Ginkgo biloba, Ginkgo biloba, Chamomile, Roman chamomile, Carrot, Gentian, Burdock, Rice, Hawthorn, Shiitake, Ginger, Hawthorn, Prunus edulis , Senkyu, assembly, thyme, chopsticks, chimpanzee, chili pepper, touki, toonin, spruce, carrot, garlic, butcherbroom, grape, button, marronie, melissa, yuzu, yokuinin, ryokucha, rosemary, rosehip, chimpo, touki, Ingredients derived from spruce, peach, apricot, walnut, corn etc); acetylcholine, ictamol, cantaristinki, gamma-oryzanol, cepharantine, trazoline, nicotinic acid toco Eroru, hesperidin, and the like.
　［美容組成物の製造方法］
　本発明の美容組成物の製造方法は特に制限されず、必須成分であるＩＰＬ照射様作用剤、必要に応じて配合されるその他の成分（上記任意成分、後述する基剤又は担体、添加剤等）を適宜選択、配合して、常法により製造することができる。 [Method of producing cosmetic composition]
The method for producing the cosmetic composition of the present invention is not particularly limited, and the IPL irradiation-like active agent which is an essential component, other components optionally mixed (the above optional components, the base or carrier described later, additives, etc. ) Can be appropriately selected and blended, and can be produced by a conventional method.
　［美容組成物の用途］
　本発明の美容組成物は、美容医のＩＰＬ施術（光施術）着想のアプローチで、ＩＰＬ照射と同様の全顔に対する効果を期待して使用されるものである。本発明の美容組成物を用いることで、全顔の美白、スキントーンの向上効果が得られる。また、全顔のくすみ、ハリのなさ、毛穴目立ち、乾燥などのエージング症状の改善（スキントーン改善）にも効果を奏する。このように、本発明の美容組成物は、ＩＰＬ照射の代替用として、またＩＰＬ照射と併用して使用することができる。このような目的で使用する本発明の美容組成物は、例えば、美容液、化粧水、乳液、クリーム、ジェルクリーム、美容マスク（フェイスマスク）、化粧下地、ファンデーション、日焼け止め、クレンジング、洗浄剤等の外用組成物として好適に使用することができ、より好ましくは、美容液、化粧水、乳液、クリーム、ジェルクリーム等のスキンケア製品として使用することができる。 [Use of cosmetic composition]
The cosmetic composition of the present invention is an approach based on the IPL treatment (light treatment) idea of a cosmetologist and used in expectation of the same effect on the entire face as IPL irradiation. By using the cosmetic composition of the present invention, skin whitening and skin tone improvement effects can be obtained. In addition, it is also effective in improving aging symptoms (skin tone improvement) such as dullness of the entire face, lack of firmness, pore appearance and dryness. Thus, the cosmetic composition of the present invention can be used as an alternative to IPL radiation and in combination with IPL radiation. The cosmetic composition of the present invention used for such purpose is, for example, a cosmetic solution, a lotion, an emulsion, a cream, a gel cream, a cosmetic mask (face mask), a cosmetic base, a foundation, a sunscreen, a cleansing agent, etc. The composition for external use can be suitably used, and more preferably, it can be used as a skin care product such as a cosmetic solution, a lotion, an emulsion, a cream, a gel cream and the like.
　また、本発明の美容組成物は、食品組成物とすることができる。この食品組成物は、健康食品、栄養補助食品（バランス栄養食、サプリメントなどを含む）として好適に用いることができる。また、保健機能食品（特定保健用食品（疾病リスク低減表示、規格基準型を含む））に好適である。 Also, the cosmetic composition of the present invention can be a food composition. This food composition can be suitably used as a health food, a nutritional supplement (including a balanced nutritional diet, a supplement, etc.). In addition, it is suitable for health functional food (food for specified health use (including disease risk reduction indication, standard standard type)).
　［製剤（外用組成物）］
　本発明の美容組成物が外用組成物である場合は、その必須成分及び上記で説明したその他の任意成分等を、化粧品、医薬品、医薬部外品に通常使用される基剤又は担体、及び必要に応じて、後述する添加剤と共に常法に従い混合して、必要に応じて乳化又は可溶化を行い、各種の製剤形態の美容組成物とすることができる。 [Formulation (composition for external use)]
When the cosmetic composition of the present invention is a composition for external use, a base or carrier usually used for cosmetics, medicines, quasi-drugs and its essential components and other optional components described above, etc. Depending on the necessity, it mixes with an additive mentioned later according to a conventional method, and it emulsifies or solubilizes as needed, It can be set as the cosmetic composition of various formulation forms.
　上記基剤又は担体としては、例えば、流動パラフィン、スクワラン、ワセリン、ゲル化炭化水素（プラスチベース等）、オゾケライト、α－オレフィンオリゴマー、軽質流動パラフィン等の炭化水素；メチルポリシロキサン、環状シリコーン、変性シリコーン、シリコーンレジン等のシリコーン油；ヤシ油、オリーブ油、コメヌカ油、シアバター等の油脂；ホホバ油、ミウロウ、キャンデリラロウ、ラノリン等のロウ類；セタノール、セトステアリルアルコール、ステアリルアルコール、ベヘニルアルコール、オクチルドデカノール、イソステアリルアルコール、フィトステロール、コレステロール等の高級アルコール；メチルセルロース、エチルセルロース、ヒドロキシプロピルセルロース、ヒドロキシプロピルメチルセルロースポリビニルピロリドン、カラギーナン、ポリエチレングリコール、ミリスチン酸イソプロピル、ミリスチン酸オクチルドデシル、パルミチン酸イソプロピル、パルミチン酸セチル、イソノナン酸イソノニル、テトラ２－エチルヘキサン酸ペンタエリスリット等のエステル類；デキストリン、マルトデキストリン等の多糖類；エタノール等の低級アルコール；ジエチレングリコールモノエチルエーテル、等のグリコールエーテル；水等が挙げられる。 Examples of the base or carrier include hydrocarbons such as liquid paraffin, squalane, petrolatum, gelled hydrocarbon (plasti base etc.), ozokerite, α-olefin oligomer, light liquid paraffin, etc .; methyl polysiloxane, cyclic silicone, modified silicone Silicone oils such as silicone resins; Fats such as coconut oil, olive oil, rice bran oil, shea butter, etc. Waxes such as jojoba oil, milows, candelilla wax, lanolin, etc. Setanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol, octyl dodeca Higher alcohols such as nor, isostearyl alcohol, phytosterol, cholesterol; methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose polyvinyl alcohol Esters such as pyrrolidone, carrageenan, polyethylene glycol, isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, isononyl isononanoate, pentaerysylate tetra 2-ethylhexanoate; multiple substances such as dextrin and maltodextrin Sugars; lower alcohols such as ethanol; glycol ethers such as diethylene glycol monoethyl ether; water and the like.
　以上説明した基剤又は担体は、１種単独で用いてもよいし、２種以上を併用してもよい。またそれらの使用量は当業者に公知の範囲から適宜選択される。 The base or carrier described above may be used alone or in combination of two or more. In addition, the amount used thereof is appropriately selected from the range known to those skilled in the art.
　［添加剤（外用組成物）］
　本発明の美容組成物には、本発明の効果を損なわない範囲で、化粧品、医薬品、医薬部外品に添加される公知の添加剤、例えば、界面活性剤、酸化防止剤、着色剤、パール光沢付与剤、キレート剤、ｐＨ調整剤、保存剤、増粘剤、等を添加することができる。これらの添加剤は、１種単独で用いてもよいし、２種以上を併用することもできる。 [Additives (composition for external use)]
The cosmetic composition of the present invention may be a known additive added to cosmetics, pharmaceuticals, quasi-drugs, as long as the effects of the present invention are not impaired, such as surfactants, antioxidants, colorants, pearls A glossing agent, a chelating agent, a pH adjuster, a preservative, a thickener, etc. can be added. These additives may be used alone or in combination of two or more.
　上記界面活性剤としては、非イオン性界面活性剤、陽イオン性界面活性剤、陰イオン性界面活性剤、両性界面活性剤等のいずれでもよく、例えば、ソルビタンモノイソステアレート、ソルビタンモノラウレート、ソルビタンモノパルミテート、ソルビタンモノステアレート、ペンタ－２－エチルヘキシル酸ジグリセロールソルビタン、テトラ－２－エチルヘキシル酸ジグリセロールソルビタン等のソルビタン脂肪酸エステル類；モノステアリン酸プロピレングリコール等のプロピレングリコール脂肪酸エステル類；ポリオキシエチレン硬化ヒマシ油４０（ＨＣＯ－４０）、ポリオキシエチレン硬化ヒマシ油５０（ＨＣＯ－５０）、ポリオキシエチレン硬化ヒマシ油６０（ＨＣＯ－６０）、ポリオキシエチレン硬化ヒマシ油８０等の硬化ヒマシ油誘導体；モノラウリル酸ポリオキシエチレン（２０）ソルビタン（ポリソルベート２０）、モノステアリン酸ポリオキシエチレン（２０）ソルビタン（ポリソルベート６０）、モノオレイン酸ポリオキシエチレン（２０）ソルビタン（ポリソルベート８０）、イソステアリン酸ポリオキシエチレン（２０）ソルビタン等のポリオキシエチレンソルビタン脂肪酸エステル類；ポリオキシエチレンモノヤシ油脂肪酸グリセリル；グリセリンアルキルエーテル；アルキルグルコシド；ポリオキシエチレンセチルエーテル等のポリオキシアルキレンアルキルエーテル；ステアリルアミン、オレイルアミン等のアミン類；ポリオキシエチレン・メチルポリシロキサン共重合体、ラウリルＰＥＧ－９ポリジメチルシロキシエチルジメチコン、ＰＥＧ－９ポリジメチルシロキシエチルジメチコン等のシリコーン系界面活性剤等が挙げられる。 The surfactant may be any of nonionic surfactant, cationic surfactant, anionic surfactant, amphoteric surfactant and the like, and examples thereof include sorbitan monoisostearate and sorbitan monolaurate. Sorbitan monopalmitate, sorbitan monostearate, diglycerol sorbitan penta-2-ethylhexyl acid, sorbitan fatty acid esters such as diglycerol sorbitan tetra-2-ethylhexyl acid; propylene glycol fatty acid esters such as propylene glycol monostearate; Cured castors of polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil 60 (HCO-60), polyoxyethylene hydrogenated castor oil 80, etc. Oil derivatives; Monolaurate polyoxyethylene (20) sorbitan (polysorbate 20), monostearate polyoxyethylene (20) sorbitan (polysorbate 60), monooleate polyoxyethylene (20) sorbitan (polysorbate 80), isostearate Polyoxyethylene (20) polyoxyethylene sorbitan fatty acid esters such as sorbitan; polyoxyethylene mono-cocoyl oil fatty acid glyceryl; glycerin alkyl ether; alkyl glucoside; polyoxyalkylene alkyl ether such as polyoxyethylene cetyl ether; stearylamine, oleylamine Amines such as polyoxyethylene / methylpolysiloxane copolymer, lauryl PEG-9 polydimethylsiloxyethyl dimethicone, PEG- Silicone surfactants such as poly dimethicone and the like.
　上記酸化防止剤としては、例えば、ジブチルヒドロキシトルエン、ブチルヒドロキシアニソール、ソルビン酸、亜硫酸ナトリウム、アスコルビン酸、エリソルビン酸、Ｌ－システイン塩酸塩等が挙げられる。 Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, sorbic acid, sodium sulfite, ascorbic acid, erythorbic acid, L-cysteine hydrochloride and the like.
　上記着色剤としては、例えば、無機顔料、天然色素等が挙げられる。 As said coloring agent, an inorganic pigment, a natural pigment | dye etc. are mentioned, for example.
　上記キレート剤としては、例えば、ＥＤＴＡ・２ナトリウム塩、ヒロドキシエタンジホスホン酸・カルシウム・２ナトリウム塩等が挙げられる。 Examples of the chelating agent include EDTA · disodium salt, hydroxyethane diphosphonic acid · calcium · disodium salt and the like.
　上記ｐＨ調整剤としては、例えば無機酸（塩酸、硫酸等）、有機酸（乳酸、乳酸ナトリウム、クエン酸、クエン酸ナトリウム、コハク酸、コハク酸ナトリウム等）、無機塩基（水酸化カリウム、水酸化ナトリウム等）、有機塩基（トリエタノールアミン、ジイソプロパノールアミン、トリイソプロパノールアミン等）等が挙げられる。 Examples of the pH adjuster include inorganic acids (hydrochloric acid, sulfuric acid etc.), organic acids (lactic acid, sodium lactate, citric acid, sodium citrate, sodium citrate, succinic acid, sodium succinate etc.), inorganic bases (potassium hydroxide, hydroxide Sodium etc., organic bases (triethanolamine, diisopropanolamine, triisopropanolamine etc.) and the like.
　上記保存剤としては、例えば、安息香酸、安息香酸ナトリウム、デヒドロ酢酸、デヒドロ酢酸ナトリウム、パラオキシ安息香酸エチル、パラオキシ安息香酸プロピル、パラオキシ安息香酸メチル、フェノキシエタノール、カプリルヒドロキサム酸、ペンタンジオール、ヘキサンジオール、オクタンジオール等のアルカンジオール類等が挙げられる。 Examples of the preservative include benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, methyl parahydroxybenzoate, phenoxyethanol, capryl hydroxamic acid, pentanediol, hexanediol, octane Alkane diols, such as diol, etc. are mentioned.
　上記増粘剤としては、例えば、ポリビニルアルコール、ポリビニルピロリドン、カルボキシビニルポリマー等のビニル系増粘剤、メチルセルロース、エチルセルロース、ヒドロキシエチルセルロース、ヒドロキシメチルセルロース、ヒドロキシプロピルセルロース、ヒドロキシプロピルメチルセルロース、カルボキシメチルセルロース、カルボキシエチルセルロース等のセルロース系増粘剤、グアーガム、ペクチン、プルラン、ゼラチン、ローカストビーンガム、カラギーナン、寒天、キサンタンガム、アクリル酸メタクリル酸アルキル共重合体、ポリエチレングリコール、ベントナイト、アルギン酸、アルギン酸プロピレングリコール、マクロゴール、コンドロイチン硫酸ナトリウム、ヒアルロン酸、ヒアルロン酸ナトリウム、（アクリル酸ヒドロキシエチル／アクリロイルジメチルタウリンＮａ）コポリマー、（アクリロイルジメチルタウリンアンモニウム／ビニルピロリドン）コポリマー等が挙げられる。 Examples of the thickener include vinyl thickeners such as polyvinyl alcohol, polyvinyl pyrrolidone and carboxyvinyl polymer, methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose and the like. Cellulose thickeners, guar gum, pectin, pullulan, gelatin, locust bean gum, carrageenan, agar, xanthan gum, acrylic acid methacrylic acid alkyl methacrylate copolymer, polyethylene glycol, bentonite, alginic acid, propylene glycol alginate, macrogol, chondroitin sulfate Sodium, hyaluronic acid, sodium hyaluronate, Hydroxyethylmethacrylate / acryloyldimethyltaurinato Na) copolymer, and (ammonium acryloyldimethyltaurate / vinyl pyrrolidone) copolymers and the like.
　［製剤形態（外用組成物）］
　本発明の美容組成物が外用組成物である場合の製剤形態は特に限定されず、例えば、液剤（化粧水、ローション、美容液等）、クリーム製剤（乳液、クリーム、ジェルクリーム、バーム等）、ジェル製剤、ゼリー製剤、シャーベット製剤、エアゾール剤、フォーム製剤、スプレー製剤、貼付剤、スティック製剤、その他軟膏剤、固形剤等が挙げられる。これらの製剤は、常法、例えば第１７改正日本薬局方製剤総則に記載の方法等に従い製造することができる。また目的とする安定性、使用感等を考慮し、適宜、Ｗ／Ｏ、Ｏ／Ｗ、Ｗ／Ｏ／Ｗ、Ｏ／Ｗ／Ｏ型エマルション等の剤型が選択できる。なお、貼付剤は、例えば、不織布シート、ゲルシート等に、美容液組成物、化粧水組成物、乳液組成物等を含浸させたものであってもよい。 [Formulation form (composition for external use)]
The formulation form in the case where the cosmetic composition of the present invention is a composition for external use is not particularly limited, and, for example, a liquid preparation (skin lotion, lotion, serum etc.), cream preparation (milky lotion, cream, gel cream, balm etc.), Gel formulations, jelly formulations, sherbet formulations, aerosol formulations, foam formulations, spray formulations, patches, stick formulations, other ointments, solid formulations and the like can be mentioned. These preparations can be manufactured according to a conventional method, for example, the method described in the 17th revised Japanese Pharmacopoeia General Rules, and the like. In addition, agent types such as W / O, O / W, W / O / W, and O / W / O type emulsions can be appropriately selected in consideration of the desired stability, feeling in use, and the like. The patch may be, for example, a non-woven sheet, a gel sheet or the like impregnated with a cosmetic composition, a cosmetic composition, an emulsion composition or the like.
　［製剤（食品組成物）］
　本発明の美容組成物が食品組成物である場合、その必須成分及び上記で説明したその他の任意成分等を、食品に通常使用される基剤又は担体、及び必要に応じて、油脂、乳化剤、分散剤、懸濁化剤、安定剤、増粘剤、甘味料、着色剤、香料、保存料、酸化防止剤、有機酸などの食品添加剤と共に混合して、製剤化すればよい。なお、食品添加剤は、上記のもの及び後述する具体的成分に限定されるわけではなく、適宜目的や必要性に応じて選択することができる。また、これらの食品添加剤は、１種を単独で又は２種以上を組み合わせて使用できる。さらに、製剤には、その他の活性成分を添加することができる。 [Formulation (food composition)]
When the cosmetic composition of the present invention is a food composition, the essential components thereof and the other optional components described above, etc. may be used as a base or carrier generally used in food, and, if necessary, fats and oils, emulsifiers, It may be formulated by mixing with food additives such as dispersants, suspending agents, stabilizers, thickeners, sweeteners, colorants, flavors, preservatives, antioxidants, organic acids and the like. The food additive is not limited to the above-described ones and the specific components described later, and can be selected appropriately according to the purpose and necessity. Moreover, these food additives can be used individually by 1 type or in combination of 2 or more types. In addition, other active ingredients can be added to the formulation.
　上記油脂としては、落花生油、ココアバター、コメ胚芽油、シソ油、亜麻仁油などの天然植物油等やこれらの硬化油、脂肪酸（中鎖脂肪酸を含む）のグリセリド（グリセリド、ジグリセリド、トリグリセリドなど）、ミツロウ等が挙げられる。中でも、ミツロウが好ましい。 The above fats and oils include natural vegetable oils such as peanut oil, cocoa butter, rice germ oil, perilla oil, linseed oil, etc., and hydrogenated oils thereof, glycerides of fatty acids (including medium-chain fatty acids) (glycerides, diglycerides, triglycerides, etc.) The bee wax etc. are mentioned. Among them, beeswax is preferable.
　上記乳化剤、分散剤、懸濁化剤、安定剤としては、ポリエチレングリコール、プロピレングリコール、グリセリン、ソルビトールのような多価アルコール；グリセリン脂肪酸エステル；カルボキシメチルセルロースナトリウム等が挙げられる。中でも、グリセリン、グリセリン脂肪酸エステル、カルボキシメチルセルロースナトリウムが好ましい。 Examples of the emulsifying agent, dispersing agent, suspending agent, and stabilizing agent include polyethylene glycol, propylene glycol, glycerin, polyhydric alcohol such as sorbitol, sorbitol; glycerin fatty acid ester; sodium carboxymethylcellulose and the like. Among them, glycerin, glycerin fatty acid ester and carboxymethylcellulose sodium are preferable.
　上記増粘剤としては、ローカストビーンガム、グアガム、キサンタンガム、タマリンドガム、ペクチン、カラギーナンなどが挙げられる。中でも、キサンタンガムが好ましい。 Examples of the thickener include locust bean gum, guar gum, xanthan gum, tamarind gum, pectin, carrageenan and the like. Among them, xanthan gum is preferred.
　上記甘味料としては、ショ糖、果糖、麦芽糖、エリスリトール、トレハロース、マルチトール、甘草抽出物、ステビア加工甘味料、アスパルテーム、アセスルファムカリウム、ソルビトール、キシリトールなどが挙げられる。 As the above-mentioned sweetener, sucrose, fructose, maltose, erythritol, trehalose, maltitol, licorice extract, stevia processed sweetener, aspartame, acesulfame potassium, sorbitol, xylitol and the like can be mentioned.
　上記保存料としては、安息香酸又はその塩、ソルビン酸又はその塩、パラオキシ安息香酸ブチル、パラオキシ安息香酸イソブチル、パラオキシ安息香酸イソプロピル、パラオキシ安息香酸エチル、パラオキシ安息香酸メチルなどが挙げられる。 Examples of the preservative include benzoic acid or a salt thereof, sorbic acid or a salt thereof, butyl parahydroxybenzoate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, ethyl parahydroxybenzoate, methyl parahydroxybenzoate and the like.
　上記酸化防止剤としては、アスコルビン酸、アスコルビン酸ナトリウム、アスコルビン酸ステアリン酸エステル、アスコルビン酸ステアリン酸ナトリウム、アスコルビン酸パルミチン酸エステル、亜硫酸水素塩、次亜硫酸ナトリウム、二酸化硫黄、ＥＤＴＡカルシウム二ナトリウム、エリソルビン酸、エリソルビン酸ナトリウム、トコフェロールなどが挙げられる。中でも、アスコルビン酸、アスコルビン酸パルミチン酸エステル、トコフェロールが好ましい。 As the above antioxidant, ascorbic acid, sodium ascorbate, ascorbic acid stearic acid ester, sodium ascorbic acid stearic acid ester, ascorbic acid palmitic acid ester, hydrogen sulfite, sodium hyposulfite, sulfur dioxide, calcium EDTA disodium, erythorbic acid Sodium erythorbate, tocopherol and the like. Among them, ascorbic acid, ascorbic acid palmitate ester, and tocopherol are preferable.
　有機酸としては、クエン酸、コハク酸、酒石酸、アスパルギン酸、乳酸、リンゴ酸、マロン酸、フマル酸、マレイン酸などが挙げられる。 Examples of the organic acid include citric acid, succinic acid, tartaric acid, aspartic acid, lactic acid, malic acid, malonic acid, fumaric acid and maleic acid.
　［ｐＨ］
　本発明の美容組成物のｐＨは、通常ｐＨ３．０～８．０であり、ｐＨ３．５～７．５であることが好ましい。なお、このｐＨは、例えば後述するｐＨ調整剤の使用により調整することができる。ただし、ｐＨ測定が不能又は困難な製剤形態については、この限りではない。 [PH]
The pH of the cosmetic composition of the present invention is usually 3.0 to 8.0, preferably 3.5 to 7.5. In addition, this pH can be adjusted, for example by use of the pH adjuster mentioned later. However, it is not this limitation about the formulation form which is impossible or difficult to measure pH.
＜スクリーニング方法＞
　本発明は、スキントーン向上用成分のスクリーニング方法、スキントーン改善用成分のスクリーニング方法も含む。本発明のスクリーニング方法は、皮膚細胞にＩＰＬ照射することで発現が変化する遺伝子を指標にすることを特徴とした方法であり、皮膚培養細胞に被験物質を作用させる工程（１）、上記皮膚培養細胞における上記遺伝子発現を測定する工程（２）を少なくとも含む。本発明のスクリーニング方法によると、ＩＰＬ照射（光施術）を受けた場合と同様の効果が得られるスキントーン向上用成分、スキントーン改善成分を効率的に得ることができる。 <Screening method>
The present invention also includes a method of screening a skin tone improving component and a method of screening a skin tone improving component. The screening method of the present invention is a method characterized by using a gene whose expression is changed by irradiating IPL to skin cells as an indicator, and a step (1) of causing a test substance to act on skin culture cells, the above-mentioned skin culture At least a step (2) of measuring the gene expression in the cell. According to the screening method of the present invention, it is possible to efficiently obtain a skin tone improving component and a skin tone improving component that can obtain the same effect as when IPL irradiation (phototreatment) is received.
　工程（１）：上記皮膚培養細胞としては、メラニン細胞を含む細胞集団であれば特に限定されないが、例えば表皮細胞、真皮細胞、培養皮膚モデル等を用いることができる。上記培養皮膚モデルとして、ＭＥＬ－３００Ａ（ＭａｔＴｅｋ社）やＥＦＴ－４００（ＭａｔＴｅｋ社）などの使用がより好ましい。上記皮膚培養細胞に被験物質を添加し、２時間から７２時間程度培養する。陰性対照としては、被験物質を添加していないものを用意する。 Step (1): The skin culture cell is not particularly limited as long as it is a cell population containing melanocytes. For example, epidermal cells, dermal cells, a cultured skin model and the like can be used. As the above-mentioned cultured skin model, use of MEL-300A (MatTek) or EFT-400 (MatTek) is more preferable. The test substance is added to the above-mentioned cultured skin cells and cultured for about 2 to 72 hours. As a negative control, one to which no test substance is added is prepared.
　工程（２）：上記工程（１）の後、細胞を回収し、常法に従ってＲＮＡを抽出し、ＤＮＡマイクロアレイ法等にて遺伝子発現を解析する。工程（１）において被験物質を添加した群と添加しなかった群（陰性対照）のそれぞれの遺伝子発現を対応させる。この時、対応させる遺伝子としては、皮膚細胞にＩＰＬ照射することで変動が起こることが確認できているものを用いる。このような遺伝子としては、例えば、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ２、ＨＢ‐ＥＧＦが挙げられる。これらの遺伝子は、ＩＰＬ照射により発現が抑制又は促進されることが本発明者らにより見出された遺伝子である。本発明のスクリーニング方法においては、被験物質の添加群において、各遺伝子の発現がＩＰＬ照射の際と同様に変動するか否かを確認する。そして、ＩＰＬ照射の際と同様にこれらの遺伝子の発現を変動させる被験物質を、スキントーン向上用成分、スキントーン改善用成分と判定することができる。 Step (2): After the above step (1), cells are recovered, RNA is extracted according to a conventional method, and gene expression is analyzed by a DNA microarray method or the like. The gene expression of each of the group to which the test substance was added and the group to which no test substance was added (negative control) in step (1) is matched. At this time, as a gene to be matched, a gene whose fluctuation has been confirmed to occur by irradiating IPL to skin cells is used. Such genes include, for example, OCA2, SLC45A2, RAB27A2, and HB-EGF. These genes are genes found by the present inventors to be suppressed or promoted in expression by IPL irradiation. In the screening method of the present invention, in the addition group of the test substance, it is confirmed whether the expression of each gene fluctuates as in the IPL irradiation. And the test substance which changes expression of these genes similarly to the time of IPL irradiation can be determined as the component for skin tone improvement, and the component for skin tone improvement.
＜美容方法＞
　本発明は、ＩＰＬ照射様作用剤を使用することを特徴とする、皮膚状態を改善・向上させるための美容方法も含む。本発明の美容方法によると、ＩＰＬ照射様作用剤を使用することで、ＩＰＬ照射と同様の効果を得られるため、例えば、全顔の美白、スキントーンの向上効果が得られる。また、全顔のくすみ、肌理の乱れ、小じわ、毛細血管拡張症などのエージング症状の改善効果（スキントーン改善効果）が得られる。なお、上記ＩＰＬ照射様作用剤の具体的な説明は、上述の美容組成物の項における説明をそのまま適用できる。 <Beauty method>
The present invention also includes cosmetic methods for improving and improving skin conditions, characterized by using an IPL radiation-like agent. According to the cosmetic method of the present invention, the same effect as IPL irradiation can be obtained by using the IPL irradiation-like active agent, and thus, for example, the effect of improving skin whitening and skin tone can be obtained. In addition, the improvement effect (skin tone improvement effect) of aging symptoms such as the dullness of the whole face, texture disorder, fine wrinkles, and telangiectasia can be obtained. In addition, the description in the term of the above-mentioned cosmetic composition can be applied as it is as the specific description of the above-mentioned IPL irradiation-like agent.
＜ＩＰＬ照射様作用剤＞
　本発明は、各種植物抽出物を有効成分として含有するＩＰＬ照射様作用剤も含む。上記植物抽出物としては、フラボノイドを含有する植物抽出物、海藻抽出物が好ましい。また、上記フラボノイドを含有する植物抽出物としては、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、ローズマリーエキス、アルテアエキス及びゴボウエキスからなる群より選択される少なくとも一種の植物抽出物がより好ましい。上記海藻抽出物としては、褐藻（ガゴメコンブ、リシリコンブ、ミツイシコンブ、マコンブ、ナガコンブ、ワカメ等）、紅藻（テングサ等）、緑藻（アオノリ等）、藍藻（スピルリナ等）等が挙げられ、中でも褐藻がより好ましい。 <IPL irradiation-like agent>
The present invention also includes IPL radiation-like agents containing various plant extracts as active ingredients. As said plant extract, the plant extract containing a flavonoid and a seaweed extract are preferable. Moreover, as a plant extract containing the above-mentioned flavonoids, amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, albino bark extract, Melia azalacilla leaf extract, mandarin orange peel extract, yukinoshita extract, horseradish seed It is more preferable to use at least one plant extract selected from the group consisting of an extract, an extract of Bemisia sinensis and a hop flower extract, a leaf extract of Tamblyssa trichophila, a rosemary extract, an altea extract and a burdock extract. Examples of the seaweed extract include brown algae (gagome kombu, lysiensis, mitsushikonbu, maconbu, nagacomb, wakame etc.), red algae (eg tenegusa etc.), green algae (eg Aonori etc.), blue-green algae (spyrulina etc.) etc. preferable.
　即ち、本発明のＩＰＬ照射様作用剤としては、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス及びタンブリッサトリコフィラ葉エキス、ローズマリーエキス、ゴボウエキス、及びアルテアエキスから成る群より選択される少なくとも一種の植物抽出物を有効成分として含むものが好ましい。また、本発明のＩＰＬ照射様作用剤としては、褐藻、紅藻、緑藻、及び藍藻から成る群より選択される少なくとも一種の海藻抽出物を有効成分として含むものが好ましく、褐藻の海藻抽出物を有効成分として含むものがより好ましい。 That is, as the IPL irradiation-like active agent of the present invention, amacha persimmon extract, artichoke extract, licorice root extract, neybara fruit extract, albino bark extract, Meria azadia lacta leaf extract, mandarin orange peel extract, yukinoshita extract, horse mackerel seeds It is preferable to contain, as an active ingredient, at least one plant extract selected from the group consisting of an extract, a fruit extract of Bemisia sinensis L., a hop flower extract, and a extract of Tambrissa trichophila leaf extract, a rosemary extract, a burdock extract, and an altea extract. Moreover, as the IPL irradiation-like active agent of the present invention, one containing at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue algae as an active ingredient is preferable, and seaweed extracts of brown algae are preferable. It is more preferable to contain it as an active ingredient.
　本発明のＩＰＬ照射様作用剤が有効成分として含有する植物抽出物、海藻抽出物についての詳細は、上述の美容組成物の項におけるＩＰＬ照射様作用剤についての説明を適用できる。 For the details of the plant extract and the seaweed extract that the IPL radiation-like agent of the present invention contains as an active ingredient, the description of the IPL radiation-like agent in the section of the above cosmetic composition can be applied.
　本発明のＩＰＬ照射様作用剤によると、ＩＰＬ照射と同様の効果を得られるため、これを含む美容組成物を用いると、例えば、全顔の美白、スキントーンの向上効果、全顔のくすみ、ハリのなさ、毛穴目立ち、乾燥などのエージング症状の改善効果（スキントーンの改善効果）が得られる。なお、上記ＩＰＬ照射様作用剤の具体的な説明は、上述の美容組成物の項における説明をそのまま適用できる。 According to the IPL radiation-like active agent of the present invention, since the same effect as IPL radiation can be obtained, for example, skin whitening of all faces, skin tone improving effect, all faces dullness, using a cosmetic composition containing this An improvement effect (amelioration effect of skin tone) of aging symptoms such as no firmness, pore appearance and dryness is obtained. In addition, the description in the term of the above-mentioned cosmetic composition can be applied as it is as the specific description of the above-mentioned IPL irradiation-like agent.
　以下、実施例により本発明をより詳細に説明するが、本発明はこれらにより何ら限定されない。 Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited thereto at all.
［試験１］ＩＰＬ照射による表皮細胞内遺伝子発現の変化の解析
　ヒトメラノサイトを含む表皮モデル（ＭａｔＴｅｋ社；ＭＥＬ－３００Ａ）を使用して以下の試験を行った。すなわちＭＥＬ－３００Ａ到着後にＣＯ２濃度を５％に設定したインキュベータ内で、３７℃で順化し、液体培地（クラボウ社：ＥＰＩ－１００ＬＬＭＭ）にて培養を開始する。順化培養終了後、トランスウェル内の表皮シートを切り出し、液体培地を含ませたガーゼに載せ実際の施術に則した条件下で照射を行った。上記方法によって調製した表皮モデルにλ５６０ｎｍのフィルターを用いた光（ＩＰＬ５６０ｎｍ）を３ミリ秒照射後、２０ミリ秒おいてさらに３ミリ秒の照射を行い、６時間後及び２４時間後に細胞を回収した。なお、ＩＰＬ照射は、フォトフェイシャルＭ２２（ルミナス社製）を用いて行った。それぞれの細胞から常法にしたがってＲＮＡを抽出し、ＤＮＡマイクロアレイ法にて遺伝子発現を解析した。ＤＮＡマイクロアレイは、Ｈｕｍａｎ　Ｇｅｎｅ　２．０ＳＴ　Ａｒｒａｙを使用した。 [Test 1] Analysis of change in gene expression in epidermal cells by IPL irradiation The following test was performed using a skin model (MatTek; MEL-300A) containing human melanocytes. That is, after arrival of MEL-300A, the cells are conditioned at 37 ° C. in an incubator whose CO 2 concentration is set to 5%, and culture is started in a liquid medium (Kurabo: EPI-100LLMM). After completion of the acclimatization culture, the epidermal sheet in the transwell was cut out, placed on gauze containing a liquid medium, and irradiated under the conditions according to the actual treatment. The epidermal model prepared by the above method was irradiated with light (IPL 560 nm) using a filter of λ 560 nm for 3 ms, and then irradiated for 20 ms with another 3 ms, and cells were recovered after 6 hours and 24 hours . In addition, IPL irradiation was performed using Photofacial M22 (made by Luminous Corporation). RNA was extracted from each cell according to a conventional method, and gene expression was analyzed by the DNA microarray method. The DNA microarray used Human Gene 2.0 ST Array.
　ＩＰＬ５６０ｎｍ照射によって表皮細胞中で発現変動のあった遺伝子として、ＯＣＡ２、ＲＡＢ２７Ａ、ＳＬＣ４５Ａ２、ＨＢ－ＥＧＦが選定された。 OCA2, RAB27A, SLC45A2, and HB-EGF were selected as genes whose expression was changed in epidermal cells by IPL 560 nm irradiation.
［試験２］ヒトメラノサイトでのＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ遺伝子の発現に影響を与える物質のスクリーニング
　正常ヒト成人表皮メラニン細胞（クラボウ社：ＮＨＥＭ）を、メラニン細胞用培地（クラボウ社：ＤｅｒｍａＬｉｆｅ　Ｍａ）を用いて、２４ウェルプレート（ＣＯＲＮＩＮＧ）に４００００ｃｅｌｌｓ／ｗｅｌｌで播種し、２日間培養した。その後、各エキスサンプルを各濃度になるように培地で調製し、ポアサイズ０．２μｍのフィルターに通したものを、培地を取り除いた２４ウェルプレートに４００μＬ／ｗｅｌｌで添加し、さらに２４時間培養後、細胞を回収した。常法によりＲＮＡを抽出し、逆転写反応によりＲＮＡからｃＤＮＡを合成し、定量リアルタイムＰＣＲにより、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａの各遺伝子発現量を定量した。得られた値を用いてブランクに対する割合を算出した。なお、ブランクとは、各エキスサンプルを添加していない培地のみによるコントロール試験のデータをいう。結果を下記表１～１７に示す。 [Test 2] Screening of substances that affect the expression of OCA2, SLC45A2 and RAB27A genes in human melanocytes Normal human adult epidermal melanocytes (Kurabo: NHEM) are used as a melanocyte culture medium (Kurabo: DermaLife Ma) Then, the cells were seeded at 40000 cells / well in a 24-well plate (CORNING) and cultured for 2 days. Thereafter, extract samples are prepared with medium to give each concentration, and those which are passed through a filter with a pore size of 0.2 μm are added at 400 μl / well to a 24-well plate from which the medium has been removed, and cultured for another 24 hours. The cells were harvested. The RNA was extracted by a conventional method, cDNA was synthesized from the RNA by reverse transcription reaction, and the expression amounts of the OCA2, SLC45A2 and RAB27A genes were quantified by quantitative real-time PCR. The ratio to blank was calculated using the obtained value. In addition, a blank means the data of the control test by only the culture medium which has not added each extract sample. The results are shown in Tables 1 to 17 below.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000012
Figure JPOXMLDOC01-appb-T000012
Figure JPOXMLDOC01-appb-T000013
Figure JPOXMLDOC01-appb-T000013
Figure JPOXMLDOC01-appb-T000014
Figure JPOXMLDOC01-appb-T000014
Figure JPOXMLDOC01-appb-T000015
Figure JPOXMLDOC01-appb-T000015
Figure JPOXMLDOC01-appb-T000016
Figure JPOXMLDOC01-appb-T000016
Figure JPOXMLDOC01-appb-T000017
Figure JPOXMLDOC01-appb-T000017
［試験３］ヒトケラチノサイトでのＨＢ－ＥＧＦ遺伝子の発現に影響を与える物質のスクリーニング
　正常ヒト成人表皮細胞（クラボウ社：ＮＨＥＫ）を、表皮角化細胞用培地（クラボウ社：Ｈｕｍｅｄｉａ　ＫＧ２）を用いて、２４ウェルプレート（ＣＯＲＮＩＮＧ）に４００００ｃｅｌｌｓ／ｗｅｌｌで播種し、１日間培養した。その後、各エキスサンプルを各濃度になるように培地で調製し、ポアサイズ０．２μｍのフィルターに通したものを、培地を取り除いた２４ウェルプレートに４００μＬ／ｗｅｌｌで添加し、さらに２４時間培養後、細胞を回収した。常法によりＲＮＡを抽出し、逆転写反応によりＲＮＡからｃＤＮＡを合成し、定量リアルタイムＰＣＲにより、ＨＢ－ＥＧＦの各遺伝子発現量を定量した。得られた値を用いてブランクに対する割合を算出した。なお、ブランクとは、各エキスサンプルを添加していない培地のみによるコントロール試験のデータをいう。結果を下記表１８に示す。 [Test 3] Screening of substances that affect the expression of HB-EGF gene in human keratinocytes Normal human adult epidermal cells (Kurabo: NHEK) using epidermal keratinocyte culture medium (Kurabo: Humedia KG2) The cells were seeded at 40000 cells / well in a 24-well plate (CORNING) and cultured for 1 day. Thereafter, extract samples are prepared with medium to give each concentration, and those which are passed through a filter with a pore size of 0.2 μm are added at 400 μl / well to a 24-well plate from which the medium has been removed, and cultured for another 24 hours. The cells were harvested. The RNA was extracted by a conventional method, cDNA was synthesized from the RNA by reverse transcription reaction, and the expression level of each gene of HB-EGF was quantified by quantitative real time PCR. The ratio to blank was calculated using the obtained value. In addition, a blank means the data of the control test by only the culture medium which has not added each extract sample. The results are shown in Table 18 below.
Figure JPOXMLDOC01-appb-T000018
Figure JPOXMLDOC01-appb-T000018
　以上から、本発明の美容組成物及び美容方法は、本来より肌色のトーンが濃い肌を明るく導く効果（スキントーンの向上効果）も期待でき、本発明の美容組成物は、スキントーン向上剤としても使用できる。また、本発明の美容組成物及び美容方法は、全顔のくすみ、ハリのなさ、毛穴目立ち、乾燥等のエージング症状の改善効果（スキントーン改善効果）も期待でき、スキントーン改善剤としても使用できる。 From the above, the cosmetic composition and the cosmetic method of the present invention can also be expected to have an effect (bright skin tone improving effect) to lighten skin with a darker skin tone than originally expected (a cosmetic composition of the present invention can be used as a skin tone improver). Can also be used. In addition, the cosmetic composition and the cosmetic method of the present invention can also be expected to have the effect of improving aging symptoms (skin tone improving effect) such as dullness of the entire face, lack of firmness, pore appearance, dryness, etc. it can.
　以下に、本発明の美容組成物の製剤処方例を示す。 Below, the formulation formulation example of the cosmetic composition of this invention is shown.
処方例１
（美容液）
Ｌ－アスコルビン酸　　　　　　　　　　　　　　　　　１０．０（w/w％)
ハイドロキノン　　　　　　　　　　　　　　　　　　　　０．１
ジエチレングリコールモノエチルエーテル　　　　　　　５１．０
プロピレングリコール　　　　　　　　　　　　　　　　２３．０
アマチャヅルエキス　　　　　　　　　　　　　　　　　　０．１
ノイバラ果実エキス　　　　　　　　　　　　　　　　　　０．５
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 1
(Beauty fluid)
L-ascorbic acid 10.0 (w / w%)
Hydroquinone 0.1
Diethylene glycol monoethyl ether 51.0
Propylene glycol 23.0
Horse mackerel extract 0.1
Neubara Fruit Extract 0.5
Total purified water remaining 100.0%
処方例２
（美容液）
３－Ｏ－エチルアスコルビン酸　　　　　　　　　　　　　３．０（w/w％)
ジエチレングリコールモノエチルエーテル　　　　　　　３０．０
プロピレングリコール　　　　　　　　　　　　　　　　２０．０
グリセリン　　　　　　　　　　　　　　　　　　　　　　４．０
アルテアエキス　　　　　　　　　　　　　　　　　　　　１．０
アーティチョークエキス　　　　　　　　　　　　　　　１０．０
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 2
(Beauty fluid)
3-O-ethyl ascorbic acid 3.0 (w / w%)
Diethylene glycol monoethyl ether 30.0
Propylene glycol 20.0
Glycerin 4.0
Altea extract 1.0
Artichoke extract 10.0
Total purified water remaining 100.0%
処方例３
（化粧水）
トラネキサム酸　　　　　　　　　　　　　　　　　　　　１．５（w/w％)
サリチル酸　　　　　　　　　　　　　　　　　　　　　　０．１
ヒアルロン酸ナトリウム　　　　　　　　　　　　　　　　０．２
グリセリン　　　　　　　　　　　　　　　　　　　　　　２．０
１，３－ブチレングリコール　　　　　　　　　　　　　１０．０
ポリオキシプロピレンメチルグルコシド　　　　　　　　　０．２
アセチル化ヒアルロン酸ナトリウム　　　　　　　　　　　０．０１
ヒドロキシエチルセルロース　　　　　　　　　　　　　　０．０５
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．１５
着香剤　　　　　　　　　　　　　　　　　　　　　　　　０．０３
油溶性甘草エキス　　　　　　　　　　　　　　　　　　　０．０００１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 3
(Skin lotion)
Tranexamic acid 1.5 (w / w%)
Salicylic acid 0.1
Sodium hyaluronate 0.2
Glycerin 2.0
1,3-butylene glycol 10.0
Polyoxypropylene methyl glucoside 0.2
Acetylated sodium hyaluronate 0.01
Hydroxyethyl cellulose 0.05
Methyl parahydroxybenzoate 0.15
Flavoring agent 0.03
Oil-soluble licorice extract 0.0001
Total purified water remaining 100.0%
処方例４
（化粧水）
トラネキサム酸　　　　　　　　　　　　　　　　　　　　１．５（w/w％)
サリチル酸　　　　　　　　　　　　　　　　　　　　　　０．１
ヒアルロン酸ナトリウム　　　　　　　　　　　　　　　　０．２
グリセリン　　　　　　　　　　　　　　　　　　　　　　１．０
ジグリセリン　　　　　　　　　　　　　　　　　　　　　０．５
１，３－ブチレングリコール　　　　　　　　　　　　　　５．０
ジプロピレングリコール　　　　　　　　　　　　　　　　３．０
トレハロース　　　　　　　　　　　　　　　　　　　　　０．１
アセチル化ヒアルロン酸ナトリウム　　　　　　　　　　　０．０１
ヒドロキシエチルセルロース　　　　　　　　　　　　　　０．０５
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．１５
着香剤　　　　　　　　　　　　　　　　　　　　　　　　０．０３
海藻エキス　　　　　　　　　　　　　　　　　　　　　　０．５
ローズマリーエキス　　　　　　　　　　　　　　　　　　０．３
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 4
(Skin lotion)
Tranexamic acid 1.5 (w / w%)
Salicylic acid 0.1
Sodium hyaluronate 0.2
Glycerin 1.0
Diglycerin 0.5
1,3-butylene glycol 5.0
Dipropylene glycol 3.0
Trehalose 0.1
Acetylated sodium hyaluronate 0.01
Hydroxyethyl cellulose 0.05
Methyl parahydroxybenzoate 0.15
Flavoring agent 0.03
Seaweed extract 0.5
Rosemary extract 0.3
Total purified water remaining 100.0%
処方例５（化粧水）
リン酸Ｌ－アスコルビルマグネシウム　　　　　　　　　　３．０（w/w％)
プラセンタエキス　　　　　　　　　　　　　　　　　　　０．５
トリメチルグリシン　　　　　　　　　　　　　　　　　　３．０
ヒアルロン酸　　　　　　　　　　　　　　　　　　　　　０．０５
ガゴメコンブ抽出物　　　　　　　　　　　　　　　　　　０．３
１，３－ブチレングリコール　　　　　　　　　　　　　　５．０
ポリオキシエチレン硬化ヒマシ油　　　　　　　　　　　　１．０
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．０５
セイヨウシロヤナギ樹皮エキス　　　　　　　　　　　　２０．０
カンゾウ根エキス　　　　　　　　　　　　　　　　　　　０．０１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 5 (skin lotion)
Phosphoric acid L-Ascorbyl magnesium 3.0 (w / w%)
Placenta extract 0.5
Trimethylglycine 3.0
Hyaluronic acid 0.05
Gagome kombu extract 0.3
1,3-butylene glycol 5.0
Polyoxyethylene Cured Castor Oil 1.0
Methyl parahydroxybenzoate 0.05
White willow bark extract 20.0
Fern root extract 0.01
Total purified water remaining 100.0%
処方例６（乳液）
トラネキサム酸　　　　　　　　　　　　　　　　　　　　２．０（w/w％)
β－アルブチン　　　　　　　　　　　　　　　　　　　　０．１
サリチル酸　　　　　　　　　　　　　　　　　　　　　　０．２
ヒアルロン酸ナトリウム　　　　　　　　　　　　　　　　０．２
ジプロピレングリコール　　　　　　　　　　　　　　　　５．０
１，３－ブチレングリコール　　　　　　　　　　　　　　８．０
グリセリン　　　　　　　　　　　　　　　　　　　　　　３．０
モノステアリン酸グリセリン　　　　　　　　　　　　　　１．０
カルボキシビニルポリマー　　　　　　　　　　　　　　　０．２
キサンタンガム　　　　　　　　　　　　　　　　　　　　０．０５
メドウフォーム油　　　　　　　　　　　　　　　　　　　５．０
トリ２－エチルヘキサン酸グリセリル　　　　　　　　　　５．０
メチルポリシロキサン　　　　　　　　　　　　　　　　　１．０
トリエタノールアミン　　　　　　　　　　　　　　　　　０．１
トコフェロール　　　　　　　　　　　　　　　　　　　　０．１
アセチル化ヒアルロン酸ナトリウム　　　　　　　　　　　０．０５
エデト酸ナトリウム　　　　　　　　　　　　　　　　　　０．０５
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．２
着香剤　　　　　　　　　　　　　　　　　　　　　　　　０．０５
ローズマリーエキス　　　　　　　　　　　　　　　　　　０．１
エイジツエキス　　　　　　　　　　　　　　　　　　　　０．１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 6 (milky lotion)
Tranexamic acid 2.0 (w / w%)
β-Arbutin 0.1
Salicylic acid 0.2
Sodium hyaluronate 0.2
Dipropylene glycol 5.0
1,3-butylene glycol 8.0
Glycerin 3.0
Glycerin monostearate 1.0
Carboxy vinyl polymer 0.2
Xanthan gum 0.05
Meadowfoam oil 5.0
Glyceryl tri-2-ethylhexanoate 5.0
Methylpolysiloxane 1.0
Triethanolamine 0.1
Tocopherol 0.1
Acetylated sodium hyaluronate 0.05
Sodium edetate 0.05
Methyl parahydroxybenzoate 0.2
Flavoring agent 0.05
Rosemary extract 0.1
Agetsu extract 0.1
Total purified water remaining 100.0%
処方例７（クリーム）
ニコチン酸アミド　　　　　　　　　　　　　　　　　　　１．０（w/w％)
グリセリン　　　　　　　　　　　　　　　　　　　　　　５．０
１，３－ブチレングリコール　　　　　　　　　　　　　　５．０
軽質流動パラフィン　　　　　　　　　　　　　　　　　　３．０
アクリル酸メタクリル酸アルキル共重合体　　　　　　　　０．３
アルギニン　　　　　　　　　　　　　　　　　　　　　　０．３
ポリオキシエチレン硬化ヒマシ油　　　　　　　　　　　　０．５
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．２
メリアアザジラクタ葉エキス　　　　　　　　　　　　　　０．５
マンダリンオレンジ果皮エキス　　　　　　　　　　　　　３．０
精製水　　　　　　　　　　　　　　　　　　　　　　　適量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 7 (cream)
Nicotinamide 1.0 (w / w%)
Glycerin 5.0
1,3-butylene glycol 5.0
Light liquid paraffin 3.0
Acrylic acid methacrylic acid alkyl copolymer 0.3
Arginine 0.3
Polyoxyethylene Cured Castor Oil 0.5
Methyl parahydroxybenzoate 0.2
Melia Azadirachta Leaf Extract 0.5
Mandarin Orange Peel Extract 3.0
Purified water total amount 100.0%
処方例８（クリーム）
トラネキサム酸　　　　　　　　　　　　　　　　　　　　１．０（w/w％)
アルブチン　　　　　　　　　　　　　　　　　　　　　　３．０
サリチル酸　　　　　　　　　　　　　　　　　　　　　　０．０５
ヒアルロン酸ナトリウム　　　　　　　　　　　　　　　　０．１５
ジプロピレングリコール　　　　　　　　　　　　　　　　８．０
ジグリセリン　　　　　　　　　　　　　　　　　　　　　５．０
グリセリン　　　　　　　　　　　　　　　　　　　　　　３．０
ヒドロキシエチルウレア　　　　　　　　　　　　　　　　３．０
イソステアリン酸ポリオキシエチレンソルビタン　　　　　０．５
モノステアリン酸グリセリン　　　　　　　　　　　　　　０．４
セトステアリルグルコシド・セトステアリルアルコール　　１．５
アクリル酸・メタクリル酸アルキル共重合体　　　　　　　０．５
メドウフォーム油　　　　　　　　　　　　　　　　　　　０．５
スクワラン　　　　　　　　　　　　　　　　　　　　　　３．０
トリ（カプリル酸／カプリン酸）グリセリル　　　　　　　１．５
メチルポリシロキサン　　　　　　　　　　　　　　　　　１．０
ベヘニルアルコール　　　　　　　　　　　　　　　　　　０．２
ステアリルアルコール　　　　　　　　　　　　　　　　　０．３
キサンタンガム　　　　　　　　　　　　　　　　　　　　０．２
水酸化ナトリウム　　　　　　　　　　　　　　　　　　　０．１
エデト酸ナトリウム　　　　　　　　　　　　　　　　　　０．１
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．２
着香剤　　　　　　　　　　　　　　　　　　　　　　　　０．１
セラミド１　　　　　　　　　　　　　　　　　　　　　　０．０１
セラミド２　　　　　　　　　　　　　　　　　　　　　　０．０１
セラミド６　　　　　　　　　　　　　　　　　　　　　　０．０１
ローズマリーエキス　　　　　　　　　　　　　　　　　　０．００１
ユキノシタエキス　　　　　　　　　　　　　　　　　　　０．０１
タンブリッサトリコフィラ葉エキス　　　　　　　　　　　０．００１
エイジツエキス　　　　　　　　　　　　　　　　　　　　５．０
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 8 (cream)
Tranexamic acid 1.0 (w / w%)
Arbutin 3.0
Salicylic acid 0.05
Sodium hyaluronate 0.15
Dipropylene glycol 8.0
Diglycerin 5.0
Glycerin 3.0
Hydroxyethylurea 3.0
Polyoxyethylene sorbitan isostearate 0.5
Glycerin monostearate 0.4
Setostearyl Glucoside ・ Setostearyl Alcohol 1.5
Acrylic acid / methacrylic acid alkyl copolymer 0.5
Meadowfoam oil 0.5
Squalane 3.0
Tri (Caprylic / Capric) Glyceryl 1.5
Methylpolysiloxane 1.0
Behenyl alcohol 0.2
Stearyl alcohol 0.3
Xanthan gum 0.2
Sodium hydroxide 0.1
Sodium edetate 0.1
Methyl parahydroxybenzoate 0.2
Flavoring agent 0.1
Ceramide 1 0.01
Ceramide 2 0.01
Ceramide 6 0.01
Rosemary extract 0.001
Yukinoshita extract 0.01
Tambrissa Tricophila leaf extract 0.001
Agetsu Extract 5.0
Total purified water remaining 100.0%
処方例９（美容液）
ニコチン酸アミド　　　　　　　　　　　　　　　　　　　５．０（w/w％)
グリセリン　　　　　　　　　　　　　　　　　　　　　　２．０
１，３－ブチレングリコール　　　　　　　　　　　　　　３．０
スクワラン　　　　　　　　　　　　　　　　　　　　　　２．０
トリエチルヘキサノイン　　　　　　　　　　　　　　　　１．０
カルボキシビニルポリマー　　　　　　　　　　　　　　　０．２
（アクリロイルジメチルタウリンアンモニウム／ビニルピロリドン）
コポリマー　　　　　　　　　　　　　　　　　　　　　　０．２
ポリオキシエチレンポリオキシプロピレンブチルエーテル
（１７Ｅ．Ｏ．）　（１７Ｐ．Ｏ．）　　　　　　　　　　０．５
ポリオキシプロピレンメチルグルコシド　　　　　　　　　０．５
トリエタノールアミン　　　　　　　　　　　　　　　　　０．１５
水素添加大豆リン脂質　　　　　　　　　　　　　　　　　０．５
パラオキシ安息香酸メチル　　　　　　　　　　　　　　　０．２
ビルベリー葉エキス　　　　　　　　　　　　　　　　　　０．０５
コンフリー葉エキス　　　　　　　　　　　　　　　　　　０．０５
精製水　　　　　　　　　　　　　　　　　　　　　　　適量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 9 (cosmetic fluid)
Nicotinamide 5.0 (w / w%)
Glycerin 2.0
1,3-butylene glycol 3.0
Squalane 2.0
Triethylhexanoin 1.0
Carboxy vinyl polymer 0.2
(Acryloyl dimethyl taurine ammonium / vinyl pyrrolidone)
Copolymer 0.2
Polyoxyethylene polyoxypropylene butyl ether (17E.O.) (17P.O.) 0.5
Polyoxypropylene methyl glucoside 0.5
Triethanolamine 0.15
Hydrogenated soybean phospholipids 0.5
Methyl parahydroxybenzoate 0.2
Bilberry leaf extract 0.05
Comfrey leaf extract 0.05
Purified water total amount 100.0%
処方例１０（美容液）
ニコチン酸アミド　　　　　　　　　　　　　　　　　　　５．０（w/w％)
ジプロピレングリコール　　　　　　　　　　　　　　　　５．０
１，３－ブチレングリコール　　　　　　　　　　　　　　５．０
軽質流動パラフィン　　　　　　　　　　　　　　　　　　２．０
メチルフェニルポリシロキサン　　　　　　　　　　　　　１．０
キサンタンガム　　　　　　　　　　　　　　　　　　　　０．５
（ＰＥＧ－２４０／デシルテトラデセス－２０／ＨＤＩ）コポリマー
　　　　　　　　　　　　　　　　　　　　　　　　　　　１．５
オレイン酸コレステリル　　　　　　　　　　　　　　　　０．２
ポリオキシエチレン硬化ヒマシ油　　　　　　　　　　　　０．５
水素添加大豆リン脂質　　　　　　　　　　　　　　　　　０．２
ペンタンジオール　　　　　　　　　　　　　　　　　　　１．０
フェノキシエタノール　　　　　　　　　　　　　　　　　０．２
マカデミアナッツ脂肪酸フィトステリル　　　　　　　　　０．１
ヒメフウロエキス　　　　　　　　　　　　　　　　　　　０．０５
カンゾウ根エキス　　　　　　　　　　　　　　　　　　　０．０５
精製水　　　　　　　　　　　　　　　　　　　　　　　適量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 10 (cosmetic fluid)
Nicotinamide 5.0 (w / w%)
Dipropylene glycol 5.0
1,3-butylene glycol 5.0
Light liquid paraffin 2.0
Methyl phenyl polysiloxane 1.0
Xanthan gum 0.5
(PEG-240 / decyltetradeces-20 / HDI) copolymer 1.5
Cholesteryl oleate 0.2
Polyoxyethylene Cured Castor Oil 0.5
Hydrogenated soybean phospholipids 0.2
Pentanediol 1.0
Phenoxyethanol 0.2
Macadamia nut fatty acid phytosteryl 0.1
Himefuuro extract 0.05
Fern root extract 0.05
Purified water total amount 100.0%
処方例１１（クリーム）
ニコチン酸アミド　　　　　　　　　　　　　　　　　　　５．０（w/w％)
グリセリン　　　　　　　　　　　　　　　　　　　　　　５．０
１，３－ブチレングリコール　　　　　　　　　　　　　１０．０
１，２－ペンタンジオール　　　　　　　　　　　　　　　０．５
植物性スクワラン　　　　　　　　　　　　　　　　　　　５．０
トリ（カプリル・カプリン酸）グリセリル　　　　　　　　１．０
メチルポリシロキサン　　　　　　　　　　　　　　　　　１．０
カルボキシビニルポリマー　　　　　　　　　　　　　　　０．４
キサンタンガム　　　　　　　　　　　　　　　　　　　　０．０５
モノステアリン酸ポリオキシエチレンソルビタン　　　　　１．５
モノステアリン酸ソルビタン　　　　　　　　　　　　　　０．５
ポリオキシエチレンセチルエーテル　　　　　　　　　　　０．２
グリセリンモノ２－エチルヘキシルエーテル　　　　　　　０．５
セラミド２　　　　　　　　　　　　　　　　　　　　　　０．０００５
セラミド６II　　　　　　　　　　　　　　　　　　　　　０．００００５
海藻エキス（１）　　　　　　　　　　　　　　　　　　　２．０％
アルテアエキス　　　　　　　　　　　　　　　　　　　　０．０１
ｐＨ調整剤　　　　　　　　　　　　　　　　　　　　適量
精製水　　　　　　　　　　　　　　　　　　　　　　適量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 11 (cream)
Nicotinamide 5.0 (w / w%)
Glycerin 5.0
1,3-butylene glycol 10.0
1,2-pentanediol 0.5
Botanical squalane 5.0
Tri (Capryl capric acid) Glyceryl 1.0
Methylpolysiloxane 1.0
Carboxy vinyl polymer 0.4
Xanthan gum 0.05
Polyoxyethylene sorbitan monostearate 1.5
Sorbitan monostearate 0.5
Polyoxyethylene cetyl ether 0.2
Glycerin mono 2-ethylhexyl ether 0.5
Ceramide 2 0.0005
Ceramide 6 II 0.00005
Seaweed extract (1) 2.0%
Altea extract 0.01
pH adjuster appropriate amount purified water total amount 100.0%
処方例１２（日焼け止め）
テトラ２－ヘキシルデカン酸アスコルビル　　　　　　　　０．１（w/w％)
トリメチルグリシン　　　　　　　　　　　　　　　　　　１．０
パラメトキシケイ皮酸２－エチルヘキシル　　　　　　　１０．０
デカメチルシクロペンタシロキサン　　　　　　　　　　２０．０
パルミチン酸オクチル　　　　　　　　　　　　　　　　１０．０
ポリオキシエチレン・メチルポリシロキサン共重合体　　　３．０
メチルハイドロジェンポリシロキサン処理
低温焼成酸化亜鉛　　　　　　　　　　　　　　　　　　１５．０
ポリアクリル酸アルキル　　　　　　　　　　　　　　　　５．０
グリセリン　　　　　　　　　　　　　　　　　　　　　　５．０
無水エタノール　　　　　　　　　　　　　　　　　　　　５．０
１，３－ブチレングリコール　　　　　　　　　　　　　　３．０
パンテノール　　　　　　　　　　　　　　　　　　　　　０．１
香料　　　　　　　　　　　　　　　　　　　　　　　　　０．１
セラミド２　　　　　　　　　　　　　　　　　　　　　　０．００１
セラミド３　　　　　　　　　　　　　　　　　　　　　　０．００１
セラミド６　　　　　　　　　　　　　　　　　　　　　　０．００１
セイヨウオオバコ種子エキス　　　　　　　　　　　　　　０．０１
クダモノトケイソウ果実エキス　　　　　　　　　　　　　０．１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 12 (sunscreen)
Ascorbyl tetra 2-hexyldecanoate 0.1 (w / w%)
Trimethylglycine 1.0
2-Methylhexyl paramethoxycinnamate 10.0
Decamethylcyclopentasiloxane 20.0
Octyl palmitate 10.0
Polyoxyethylene / methylpolysiloxane copolymer 3.0
Methylhydrogenpolysiloxane treated low temperature calcined zinc oxide 15.0
Polyalkyl acrylate 5.0
Glycerin 5.0
Absolute ethanol 5.0
1,3-butylene glycol 3.0
Panthenol 0.1
Flavoring agent 0.1
Ceramide 2 0.001
Ceramide 3 0.001
Ceramide 6 0.001
Plantain Seed Extract 0.01
Beak Passion Fruit Extract 0.1
Total purified water remaining 100.0%
処方例１３（クレンジング）
Ｌ－アスコルビン酸２－グルコシド　　　　　　　　　　　０．１（w/w％)
トリイソステアリン酸ＰＥＧ－２０グリセリル　　　　　１２．０
イソステアリン酸ポリグリセリル－１０　　　　　　　　　８．０
パルミチン酸エチルヘキシル　　　　　　　　　　　　　３０．０
トリエチルヘキサノイン　　　　　　　　　　　　　　　２０．０
ミネラルオイル　　　　　　　　　　　　　　　　　　　２５．０
ジフェニルシロキシフェニルトリメチコン　　　　　　　　１．０
ホップ花エキス　　　　　　　　　　　　　　　　　　　　０．０００１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 13 (cleansing)
L-ascorbic acid 2-glucoside 0.1 (w / w%)
PEG-20 Glyceryl Triisostearate 12.0
Polyglyceryl isostearate-10 8.0
Ethylhexyl palmitate 30.0
Triethyl hexanoin 20.0
Mineral oil 25.0
Diphenylsiloxyphenyl trimethicone 1.0
Hop flower extract 0.0001
Total purified water remaining 100.0%
処方例１４（クレンジング）
Ｌ－アスコルビン酸２－グルコシド　　　　　　　　　　　０．１（w/w％)
トリイソステアリン酸ＰＥＧ－２０グリセリル　　　　　１２．０
イソステアリン酸ポリグリセリル－１０　　　　　　　　　８．０
パルミチン酸エチルヘキシル　　　　　　　　　　　　　２８．０
トリエチルヘキサノイン　　　　　　　　　　　　　　　２０．０
ミネラルオイル　　　　　　　　　　　　　　　　　　　２０．０
ジフェニルシロキシフェニルトリメチコン　　　　　　　　１．０
海藻エキス　　　　　　　　　　　　　　　　　　　　　　０．０００１
アルテアエキス　　　　　　　　　　　　　　　　　　　　０．０００１
精製水　　　　　　　　　　　　　　　　　　　　　　　残量
合計　　　　　　　　　　　　　　　　　　　　　　　１００．０％ Prescription example 14 (cleansing)
L-ascorbic acid 2-glucoside 0.1 (w / w%)
PEG-20 Glyceryl Triisostearate 12.0
Polyglyceryl isostearate-10 8.0
Ethylhexyl palmitate 28.0
Triethyl hexanoin 20.0
Mineral oil 20.0
Diphenylsiloxyphenyl trimethicone 1.0
Seaweed extract 0.0001
Altea extract 0.0001
Total purified water remaining 100.0%
処方例１５（ソフトカプセル：食品組成物）
　以下の食品組成物の処方例中の数値の単位は「重量部」である。
カプセル内容物
　月見草油　　　　　　　　　　　　　　　　　　　　１２０
　加水分解性タンニン　　　　　　　　　　　　　　　　２０
　加水分解大豆タンパク　　　　　　　　　　　　　　　２０
　ハス胚芽エキス　　　　　　　　　　　　　　　　　　　５
　セラミド　　　　　　　　　　　　　　　　　　　　　　３
　ヒアルロン酸　　　　　　　　　　　　　　　　　　　　０．５
　ＣｏＱ１０　　　　　　　　　　　　　　　　　　　　　２
　ヘマトコッカス藻色素抽出物（アスタキサンチン含有）　４
　ビタミンＣ　　　　　　　　　　　　　　　　　　　　　１
　グリセリン脂肪酸エステル　　　　　　　　　　　　　１０
　ミツロウ　　　　　　　　　　　　　　　　　　　　　　５
カプセル皮膜
　ゼラチン　　　　　　　　　　　　　　　　　　　　　９２
　プルラン　　　　　　　　　　　　　　　　　　　　　　３
　グリセリン　　　　　　　　　　　　　　　　　　　　３５
　着色料　　　　　　　　　　　　　　　　　　　　　　　３ Formulation example 15 (soft capsule: food composition)
The unit of numerical values in the following formulation examples of food compositions is "parts by weight".
Capsule Contents Evening Primrose Oil 120
Hydrolyzable tannin 20
Hydrolyzed soy protein 20
Lotus germ extract 5
Ceramide 3
Hyaluronic acid 0.5
CoQ10 2
Hematococcus alga pigment extract (containing astaxanthin) 4
Vitamin C 1
Glycerin fatty acid ester 10
Bee wax 5
Capsule film Gelatin 92
Pull Run 3
Glycerin 35
Colorant 3
処方例１６（美容ドリンク）単位（ｍｇ）
カンゾウエキス　　　　　　　　　　　　　　　　　　１８０
プラセンタ抽出物　　　　　　　　　　　　　　　　　１８０
コラーゲンペプチド　　　　　　　　　　　　　　　１０００
アーティチョークエキス　　　　　　　　　　　　　　　５０
プロテオグリカン　　　　　　　　　　　　　　　　　　２０
アマチャヅルエキス　　　　　　　　　　　　　　　　　２０
ポリソルベート８０　　　　　　　　　　　　　　　　　　０．１
エリスリトール　　　　　　　　　　　　　　　　　　　　３
寒天　　　　　　　　　　　　　　　　　　　　　　　　　０．５
水あめ　　　　　　　　　　　　　　　　　　　　　　　　０．６
果糖　　　　　　　　　　　　　　　　　　　　　　　　　２
酸味料　　　　　　　　　　　　　　　　　　　　　　　　０．５
香料　　　　　　　　　　　　　　　　　　　　　　　　　０．１
水　　　　　　　　　　　　　　　　　　　　　　　　　８６．１
１日摂取量合計（ｍｇ）　１５４２．９ Prescription example 16 (cosmetic drink) unit (mg)
Fern extract 180
Placenta extract 180
Collagen peptide 1000
Artichoke extract 50
Proteoglycan 20
Horse mackerel extract 20
Polysorbate 80 0.1
Erythritol 3
Agar 0.5
Water candy 0.6
Fructose 2
Acidulant 0.5
Flavoring agent 0.1
Water 86.1
Total daily intake (mg) 1542.9
処方例１７（美容ドリンク）単位（ｍｇ）
海藻エキス　　　　　　　　　　　　　　　　　　　　１８０
プラセンタ抽出物　　　　　　　　　　　　　　　　　１８０
コラーゲンペプチド　　　　　　　　　　　　　　　１０００
プロテオグリカン　　　　　　　　　　　　　　　　　　２０
ローズマリーエキス　　　　　　　　　　　　　　　　　４０
ポリソルベート８０　　　　　　　　　　　　　　　　　　０．１
エリスリトール　　　　　　　　　　　　　　　　　　　　３
寒天　　　　　　　　　　　　　　　　　　　　　　　　　０．５
水あめ　　　　　　　　　　　　　　　　　　　　　　　　０．６
果糖　　　　　　　　　　　　　　　　　　　　　　　　　２
酸味料　　　　　　　　　　　　　　　　　　　　　　　　０．５
香料　　　　　　　　　　　　　　　　　　　　　　　　　０．１
水　　　　　　　　　　　　　　　　　　　　　　　　１０３．２
１日摂取量合計（ｍｇ）　１５３０．０ Prescription example 17 (cosmetic drink) unit (mg)
Seaweed extract 180
Placenta extract 180
Collagen peptide 1000
Proteoglycan 20
Rosemary extract 40
Polysorbate 80 0.1
Erythritol 3
Agar 0.5
Water candy 0.6
Fructose 2
Acidulant 0.5
Flavoring agent 0.1
Water 103.2
Daily intake total (mg) 1530.0

Claims (14)

  1. 　ＩＰＬ照射様作用剤を含有する、美容組成物。 A cosmetic composition comprising an IPL radiation-like agent.
  2. 　上記ＩＰＬ照射様作用剤が、有効成分として、フラボノイドを含有する植物抽出物を少なくとも含む、請求項１に記載の美容組成物。 The cosmetic composition according to claim 1, wherein the IPL radiation-like agent at least contains a plant extract containing flavonoid as an active ingredient.
  3. 　上記植物抽出物が、ウリ科、キク科、マメ科、バラ科、ヤナギ科、センダン科、ミカン科、ユキノシタ科、オオバコ科、トケイソウ科、アサ科、モニミア科、トチノキ科、ツツジ科、ボタン科、シソ科、アオイ科、ショウガ科から成る群より選択される少なくとも一種の植物の抽出物である、請求項２に記載の美容組成物。 The above plant extract is Cucurbitaceae, Asteraceae, Leguminosae, Rosaceae, Salixaceae, Salixaceae, Fernaceae, Citrus Department, Citrusaceae, Anchovyaceae, Anchovyaceae, Acacia, Asinaceae, Moninimiaceae, Aesculusaceae, Asteraceae The cosmetic composition according to claim 2, wherein the cosmetic composition is an extract of at least one plant selected from the group consisting of Lamiaceae, mallow, and ginger.
  4. 　上記ＩＰＬ照射様作用剤が、有効成分として、海藻抽出物を少なくとも含む、請求項１に記載の美容組成物。 The cosmetic composition according to claim 1, wherein the IPL radiation-like agent at least contains a seaweed extract as an active ingredient.
  5. 　上記ＩＰＬ照射様作用剤が、皮膚細胞におけるメラニン関連分子の発現を調節する、請求項１から４のいずれか一項に記載の美容組成物。 The cosmetic composition according to any one of claims 1 to 4, wherein the IPL radiation-like agent modulates the expression of a melanin related molecule in skin cells.
  6. 　上記メラニン関連分子が、ＯＣＡ２、ＳＬＣ４５Ａ２、ＲＡＢ２７Ａ２、及びＨＢ－ＥＧＦから成る群より選択される少なくとも一種である、請求項５に記載の美容組成物。 The cosmetic composition according to claim 5, wherein the melanin related molecule is at least one selected from the group consisting of OCA2, SLC45A2, RAB27A2 and HB-EGF.
  7. 　上記植物抽出物が、アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス、タンブリッサトリコフィラ葉エキス、ローズマリーエキス、ゴボウエキス、及びアルテアエキスから成る群より選択される少なくとも一種である、請求項２、３、５、６のいずれか一項に記載の美容組成物。 The above plant extract includes amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, asterix bark extract, Meria azalea lacta leaf extract, mandarin orange peel extract, aubergine extract, a horse mackerel seed extract, a botanical fruit extract, 7. The flower according to any one of claims 2, 3, 5, 6 which is at least one selected from the group consisting of hop flower extract, Tambrissa tricophila leaf extract, rosemary extract, burdock extract, and altea extract. Beauty composition.
  8. 　更に、ターンオーバー促進剤（上記ＩＰＬ照射様作用剤を除く）、抗糖化成分及び美白有効成分からなる群より選択される少なくとも１種を含有する、請求項１から７のいずれか一項に記載の美容組成物。 Furthermore, it contains at least 1 sort (s) selected from the group which consists of a turnover promoter (except the said IPL irradiation-like active agent), an antiglycation component, and a skin-whitening active ingredient as described in any one of Claim 1 to 7 Beauty composition.
  9. 　スキントーン向上剤用である、請求項１から８のいずか一項に記載の美容組成物。 The cosmetic composition according to any one of claims 1 to 8, for skin tone enhancers.
  10. 　皮膚細胞にＩＰＬ照射することで発現が変化する遺伝子を指標として、スキントーン向上に効果的な成分をスクリーニングすることを特徴とする、スキントーン向上用成分のスクリーニング方法。 A screening method of a component for improving skin tone, comprising screening a component effective for improving skin tone by using a gene whose expression is changed by irradiating IPL to skin cells as an index.
  11. 　皮膚培養細胞に被験物質を作用させる工程、及び上記皮膚培養細胞における上記遺伝子発現を測定する工程を少なくとも含む、請求項１０に記載のスクリーニング方法。 The screening method according to claim 10, comprising at least the steps of allowing a test substance to act on skin cultured cells, and measuring the gene expression in the skin cultured cells.
  12. 　ＩＰＬ照射様作用剤を使用することを特徴とする、皮膚状態を改善・向上させるための美容方法。 A cosmetic method for improving or improving skin condition, characterized by using an IPL radiation-like agent.
  13. 　アマチャヅルエキス、アーティチョークエキス、カンゾウ根エキス、ノイバラ果実エキス、セイヨウシロヤナギ樹皮エキス、メリアアザジラクタ葉エキス、マンダリンオレンジ果皮エキス、ユキノシタエキス、セイヨウオオバコ種子エキス、クダモノトケイソウ果実エキス、ホップ花エキス及びタンブリッサトリコフィラ葉エキス、ローズマリーエキス、ゴボウエキス、及びアルテアエキスから成る群より選択される少なくとも一種の植物抽出物を有効成分として含む、ＩＰＬ照射様作用剤。 Amacha persimmon extract, artichoke extract, licorice root extract, bilberry fruit extract, albino bark extract, Meria azalea lacta leaf extract, mandarin orange peel extract, Yukinoshita extract, Eurasian plantain seed extract, abalone fruit extract fruit extract, hop flower extract and Tambrissa An IPL radiation-like agent comprising, as an active ingredient, at least one plant extract selected from the group consisting of Trichophylla leaf extract, rosemary extract, burdock extract, and altea extract.
  14. 　褐藻、紅藻、緑藻、及び藍藻から成る群より選択される少なくとも一種の海藻抽出物を有効成分として含む、ＩＰＬ照射様作用剤。 An IPL irradiation-like agent comprising, as an active ingredient, at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue-green algae.
PCT/JP2018/042581 2017-11-17 2018-11-16 Cosmetic composition, screening method, and cosmetic method WO2019098352A1 (en)

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