JPWO2019098352A1 - Cosmetological composition, screening method and cosmetological method - Google Patents

Abstract

The present invention is a cosmetological composition having a new concept, which sufficiently exerts an effect of improving skin condition comparable to IPL irradiation in terms of skin whitening, anti-aging, etc., and can be easily used by many users. The subject is to provide a new beauty method using. The present invention is a cosmetological composition containing an IPL irradiation-like agent. In addition, the cosmetological composition of the present invention has an effect comparable to that of phototherapy, and can be used as a substitute for IPL irradiation. Further, in the cosmetological composition of the present invention, it is preferable that the IPL irradiation-like agent contains at least a plant extract containing flavonoids or a seaweed extract.

Description

The present invention relates to cosmetological compositions, screening methods, cosmetological methods and IPL irradiation-like agents.

In recent years, the popularity of phototherapy has increased in the field of cosmetology. Among them, IPL (Intense Pulse Light), which is known as the device name "Photofacial (registered trademark)", "LimeLight", "Lumenis One", etc., is used to irradiate the entire face with light of a special wavelength different from that of lasers, etc. Attention is being paid to new treatment methods that can effectively improve freckles, fine lines, acne scars, redness, etc. (see Patent Document 1 and Non-Patent Document 1). IPL refers to light in a wide wavelength range that cuts short wavelengths that are harmful to the skin, such as ultraviolet rays. Specifically, it is light having a plurality of wavelengths in the wavelength range of 500 nm to 1200 nm, including a wide range of visible light and infrared rays. By irradiating light of a plurality of wavelengths in this way, various symptoms can be effectively improved. By using a filter depending on the application, light in these wavelength ranges can be irradiated by limiting the wavelength range and waveform and matching the skin symptoms. This treatment method is effective in reducing firmness, opening pores, uneven skin tone, dullness, etc., and is said to be effective in improving the aging symptoms of the entire face. Since the IPL contains light of multiple wavelengths, it is possible to irradiate the entire face instead of local irradiation such as spots and moles as in conventional laser treatment. It is believed that this problem can be effectively remedied.

However, the number of medical institutions that can receive the above-mentioned light treatment such as IPL irradiation is limited, continuous or continuous treatment is recommended depending on the skin condition to be treated, and the skin condition is not suitable for the treatment. Not everyone can easily receive it because some people have skin characteristics. However, the current situation is that the demands for skin whitening, anti-aging, etc. are increasing day by day, and there is also a demand for proposals of options according to individual lifestyles, skin conditions, skin characteristics, and the like. In addition, although phototherapy is highly effective, there are many unclear points about its mechanism of action.

Japanese Unexamined Patent Publication No. 2008-11994

Skill-up skin laser treatment (edited by Akira Kawada), Chugai Medical Co., Ltd. (published in 2011), p. 176-190

The present invention has been made in view of such a situation, clarifies the mechanism of action of phototherapy, and sufficiently exerts the effect of improving the skin condition with respect to skin whitening, anti-aging, etc., as in the case of IPL irradiation. The challenge is to provide a new concept beauty composition that can be easily used by many users, and a new beauty method using the same.

As a result of diligent research to solve the above problems, the present inventors suppressed the expression of genes involved in melanin synthesis and melanosomes transport by irradiating epidermal cells with IPL, and found that genes involved in turnover. It was found that expression was promoted. That is, it is considered that the whitening mechanism by IPL irradiation acts on the genes involved in the above-mentioned melanin synthesis, melanosomes transport, and turnover, and by utilizing this finding, it is similar to IPL irradiation in external compositions such as cosmetics. I came up with the idea of developing a product that can realize the effect, that is, a product with an IPL concept. Based on this finding, we searched for substances that can suppress the expression of these genes whose expression is suppressed by IPL irradiation in the same way as IPL irradiation. Among many substances, plant extracts and seaweed extracts containing flavonoids were extracted. We have found that the object has an IPL irradiation-like effect. The gist of the present invention is as follows.

[1] A cosmetological composition containing an IPL irradiation-like agent.
[2] The cosmetological composition according to [1], wherein the IPL irradiation-like agent contains at least a plant extract containing flavonoids as an active ingredient.
[3] The above plant extracts are: Uridae, Asteraceae, Asteraceae, Rosaceae, Salixaceae, Sendan, Rutaceae, Saxifragaceae, Plantains, Labiatae, Asa, Monimia, Rosaceae, Ericaceae. The beauty composition according to [2], which is an extract of at least one plant selected from the group consisting of Rosaceae, Labiatae, Plantainaceae, and Saxifragaceae.
[4] The cosmetological composition according to [1], wherein the IPL irradiation-like agent contains at least a seaweed extract as an active ingredient.
[5] The cosmetological composition according to any one of [1] to [4], wherein the IPL irradiation-like agent regulates the expression of melanin-related molecules in skin cells.
[6] The cosmetological composition according to [5], wherein the melanin-related molecule is at least one selected from the group consisting of OCA2, SLC45A2, and RAB27A2.
[7] The plant extracts are Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Seiyoshiroyanagi bark extract, Melia azajirakuta leaf extract, Mandarin orange peel extract, Yukinoshita extract, Seiyouobako seed extract, Kudamonotokeisou. At least one selected from the group consisting of fruit extract, hop flower extract, tambrissa trichophylla leaf extract, rosemary extract, gobo extract, and artea extract, [2], [3], [5], [6]. ] The beauty composition according to any one of.
[8] Further, any of [1] to [7], which contains at least one selected from the group consisting of a turnover accelerator (excluding the above-mentioned IPL irradiation-like agent), an anti-glycation component and a whitening active ingredient. The beauty composition described in Crab.
[9] The cosmetological composition according to any one of [1] to [8], which is for a skin tone improver.
[10] A method for screening a component for improving skin tone, which comprises screening a component effective for improving skin tone using a gene whose expression is changed by irradiating skin cells with IPL as an index.
[11] The screening method according to [10], which comprises at least a step of allowing a test substance to act on cultured skin cells and a step of measuring the gene expression in the cultured skin cells.
[12] A cosmetological method for improving / improving a skin condition, which comprises using an IPL irradiation-like agent.
[13] Amachazuru extract, artichoke extract, kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, Melia azajirakuta leaf extract, Mandarin orange bark extract, Yukinoshita extract, Oobako seed extract, Kudamonotokeisou fruit extract, Hop flower extract , Tambrissa trichophylla leaf extract, rosemary extract, gobo extract, and an IPL irradiation-like agent containing at least one plant extract selected from the group consisting of Artea extract as an active ingredient.
[14] An IPL irradiation-like agent containing at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue-green algae as an active ingredient.

According to the present invention, with respect to skin whitening, anti-aging, etc., a cosmetological composition having a new concept, which can sufficiently exert the same skin condition improving effect as IPL irradiation and can be easily used by many users, can be obtained. Can be provided. It is also possible to provide a new beauty method using the same.

Hereinafter, the present invention will be described in detail. Unless otherwise specified, the terms used in the present specification are interpreted in the meanings commonly used in the art.

<Cosmetology composition>
The cosmetological composition of the present invention contains an IPL irradiation-like agent. Here, the IPL irradiation-like agent refers to a composition capable of giving the same effect as when the subject is irradiated with IPL. This is a composition that can be an alternative to phototherapy such as IPL irradiation, that is, a cosmetological composition that is an alternative to IPL irradiation. The IPL irradiation-like agent may give a part of the effects obtained by irradiating the subject with the IPL. Although the cosmetological composition of the present invention is effective by itself, it is possible to improve the effect of IPL irradiation and maintain it for a long time by combining it with a light treatment such as IPL irradiation.

The effects obtained by irradiating the subject with IPL include, for example, the effect of regulating the expression of melanin-related molecules in skin cells, the effect of whitening the entire face, and the effect of improving skin tone. In addition, the effect of improving aging symptoms such as dullness of the entire face, lack of firmness, conspicuous pores, uneven skin tone, and dryness (skin tone improving effect) can be mentioned. Examples of the melanin-related molecule (gene and / or protein) include OCA2, SLC45A2, RAB27A2, HB-EGF and the like. By IPL irradiation, the expression of OCA2, SLC45A2, and RAB27A2 among these molecules is regulated in a direction of suppressing. In addition, among these molecules, the expression of HB-EGF is regulated in a direction that promotes it. That is, the cosmetological composition of the present invention may be at least one cosmetological composition for suppressing gene expression selected from the group consisting of OCA2, SLC45A2 and RAB27A2, or a cosmetological composition for promoting gene expression of HB-EGF. I can say. Further, since the beauty composition of the present invention suppresses or promotes the above-mentioned gene expression, it can be said that it is a beauty composition for skin tone improvement and / or skin tone improvement similar to IPL irradiation.

The cosmetological composition of the present invention may contain, in addition to the above-mentioned IPL irradiation-like agent, other optional components as long as the effects of the present invention are not impaired. The IPL irradiation-like agent and other optional components will be described below.

[IPL irradiation-like agent]
In the beauty composition of the present invention, the IPL irradiation-like agent is not particularly limited as long as it is a substance capable of giving the same effect as when the subject is irradiated with IPL, but for example, various plant extracts and the like are effective. Examples thereof include those contained as an ingredient, and among them, those containing at least a plant extract containing flavonoids are preferable. It is also preferable that it contains at least a seaweed extract.

Plant extracts containing flavonoids include, for example, Rosaceae, Asteraceae, Asteraceae, Rosaceae, Salixaceae, Sendan, Rutaceae, Saxifragaceae, Plantains, Ericaceae, Asa, Monimia, Ericaceae. , Asteraceae, Rosaceae, Labiatae, Plantains, Saxifragaceae and other plant extracts.

Examples of the above-mentioned plant extracts include Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, Melia azalea leaf extract, Mandarin orange peel extract, Yukinoshita extract, Seiyouobako seed extract, and Kudamonotokeisou. Examples thereof include fruit extract, hop flower extract, tambrissa trichophylla leaf extract, fuyubodaiju flower extract, hydrolyzed soybean peptide, blue-green algae bark extract, isayoibara extract, rosemary extract, gobo extract, and artea extract. Examples of the site to be extracted from the plant include leaves, flowers, fruits, roots, stems, skins, branches, fruits, pericarps, seeds and the like. At the time of extraction, the plant raw material may be used as it is, or it may be dried and used. The plant material can also be crushed and used. Further, a commercially available plant extract may be used as a raw material for cosmetics.

Seaweeds used in the above seaweed extract include, for example, brown algae (Gagomekonbu, Risilimb, Mitsuishikonbu, Macombu, Nagakombu, Wakame seaweed, etc.), red algae (Gelidiaceae, etc.), green algae (Aonori, etc.), and blue-green algae (Spirulina, etc.). Kind of extract. At the time of extraction, the seaweed raw material may be used as it is, or may be dried and used. The plant material can also be crushed and used. Further, a seaweed extract commercially available as a raw material for cosmetics may be used. These seaweeds may be used alone or in combination of two or more. Of these, the brown algae extract is preferable, the kelp extract is more preferable, and the gagome kelp extract is further preferable, from the viewpoint of being excellent in the effect of promoting the expression of melanin-related molecules.

Among these, among the melanin-related molecules, from the viewpoint of being particularly excellent in the effect of lowering the expression of the OCA2 gene, artichoke extract, kudamonotokeisou fruit extract, amachaduru extract, hydrolyzed soybean peptide (hydrolyzed soybean protein), citrus root Extracts, Neubara fruit extract, Tambrissa trichophylla leaf extract, Fuyubodaiju flower extract are preferable, and Artichoke extract, Kudamonotokeisou fruit extract, Kanzo root extract, and Neubara fruit extract are more preferable.

Among the melanin-related molecules, from the viewpoint of being particularly excellent in the effect of lowering the expression of the SLC45A2 gene, Amachazuru extract, Kanzo root extract, Neubara fruit extract, Artichoke extract, Hydrolyzed soybean peptide, Shiroyanagi bark extract, Melia azajirakuta Leaf extract, mandarin orange peel extract, yukinoshita extract, blue-green algae seed extract, and fuyubodaiju flower extract are preferable, and amachazuru extract, kanzo root extract, and neurose fruit extract are more preferable.

Among the melanin-related molecules, from the viewpoint of being particularly excellent in the effect of lowering the expression of the RAB27A2 gene, Amachazuru extract, artichoke extract, hydrolyzed soybean peptide, citrus root extract, Neubara fruit extract, Yukinoshita extract, broadleaf plantain seed extract, tan. Brissa trichophylla leaf extract, Fuyubodaiju flower extract, and Izayoi rose extract are preferable.

Among the melanin-related molecules, from the viewpoint of excellent effect of reducing the expression of any two or more of OCA2, SLC45A2, and RAB27A2, Amachazuru extract, artichoke extract, hydrolyzed soybean peptide, citrus root extract, Neubara fruit extract, Yukinoshita Extract, sardine seed extract, fuyubodaiju flower extract, sardine bark extract, mandarin orange peel extract, tambrissa trichophylla leaf extract are preferable, and from the viewpoint of excellent effect of lowering the expression of all three genes, amachazuru extract, Artichoke extract, hydrolyzed soybean peptide, citrus root extract, Neubara fruit extract, Yukinoshita extract, Oobako seed extract, and Fuyubodaiju flower extract are more preferable.

Among the melanin-related molecules, rosemary extract, seaweed extract, artea extract, kudamonotokeisou fruit extract, and gobo extract are preferable, and seaweed extract, artea extract, and so on, from the viewpoint of excellent effect of promoting the expression of HB-EGF gene. Rosemary extract is more preferred.

Considering the effects of the present invention comprehensively, the above-mentioned plant extracts in the beauty composition of the present invention include Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, and Melia azajirakuta leaf extract. , Mandarin orange peel extract, Yukinoshita extract, Strawberry seed extract, Kudamonotokeisou fruit extract, Hop flower extract, Tambrissa trichophylla leaf extract, Rosemary extract, Artea extract are preferable. Further, as the seaweed extract in the beauty composition of the present invention, a brown algae extract is preferable, a kelp extract is more preferable, and a gagome kelp extract is further preferable.

The content of the IPL irradiation-like agent in the beauty composition of the present invention is 0.0001% by weight to 20% by weight, 0.001% by weight when the IPL irradiation-like agent is the plant extract. % To 10% by weight, more preferably 0.01% by weight to 5% by weight, and even more preferably 0.05% by weight to 3% by weight. The cosmetological composition of the present invention may contain the above plant extract alone or in combination of two or more. When the cosmetological composition of the present invention contains two or more kinds of the above plant extracts, the total content of these two or more kinds of plant extracts is defined as the content of the above plant extracts.

The cosmetological method and cosmetological composition according to the present invention have an effect of improving skin tone by directly acting on genes involved in melanin synthesis and melanosomes transport, which determine skin whiteness, unlike the conventional whitening mechanism by inhibiting tyrosinase activity. (Skin tone improvement effect) is achieved.

Here, improving skin tone means improving spots, freckles, dullness and pigmentation of the skin with aging, and making the skin color more natural and uniform (skin tone improvement), as well as the original skin color. It also includes improving the skin tone of dark skin.

Optional ingredients contained in the cosmetological composition of the present invention include, for example, a whitening active ingredient, a turnover accelerator (excluding the IPL irradiation-like agent of the present invention), an antiglycation ingredient, an antioxidant ingredient, an antiaging ingredient, and an anti-inflammatory ingredient. Agents, refreshing agents, bactericides, vitamins, organic acids, moisturizing ingredients, polyhydric alcohols, scrubbing agents, UV absorbing ingredients, UV scattering ingredients, astringent ingredients, peptides or derivatives thereof, amino acids or derivatives thereof, cleaning ingredients, keratin Examples thereof include a softening component, a cell activating component, and a blood circulation promoting action component. In the cosmetological composition of the present invention, each of these components may be used alone or in combination of two or more.

Examples of the whitening active ingredient include tocopherol, vitamin C and its derivatives, arbutin, kojic acid, placenta, ellagic acid, nicotinamide, tranexamic acid and its derivatives, hydroquinone, rucinol (registered trademark), potassium 4-methoxysalicylate. Examples thereof include salts, linoleic acid and derivatives thereof.

Examples of the turnover accelerator include vitamins, keratin softening components, cell activating components, blood circulation promoting components, etc., which will be described later.

Examples of the anti-glycation component include plant extracts such as Budreja axilaris leaf extract, evening primrose oil, Amler fruit, fruit juice or extracts thereof, L-arginine, L-lysine, hydrolyzed casein, and hydrolyzable tannin. , Carnosin and the like.

Examples of the antioxidant component include components derived from plants (for example, grapes, glutathione, and comfrey); proanthocyanidins, tocopherols and their derivatives, ascorbic acid and its derivatives, hesperidin and its derivatives, ergothioneine, sulfite. Examples thereof include sodium hydrogen hydrogen, erythorbic acid and salts thereof, flavonoids, glutathione and the like.

Examples of the antiaging component include hydrolyzed soy protein, retinoids (retinol and its derivatives, retinoic acid, retinal, etc.), pangamic acid, kinetin, ursoleic acid, turmeric extract, sphingosine derivatives, silicon, silicic acid, N-. Examples thereof include methyl-L-serine and mevalonolactone.

Examples of the anti-inflammatory agent include allantoin and its derivatives, glycyrrhetinic acid and its derivatives, glycyrrhizic acid and its derivatives, salicylic acid derivatives, aminocaproic acid, azulene and its derivatives, zinc oxide, tocopherol acetate and the like.

Examples of the refreshing agent include terpenes such as menthol, camphor, geraniol, etc. (these may be d-form, l-form or dl-form); eucalyptus oil, bergamot oil, peppermint oil, cool mint oil, etc. Essential oils such as sparemint oil and camphor oil can be mentioned.

Examples of the above-mentioned bactericide include isopropylmethylphenol, salicylic acid, benzalkonium chloride, ethanol, benzethonium chloride, gluconic acid and derivatives thereof, Photosensitizer No. 101, Photosensitizer No. 201, paraben, phenoxyethanol, pyroctoolamine, myconazole and the like. Be done.

The vitamins may be either water-soluble vitamins or oil-soluble vitamins. For example, vitamin B6s, pantothenic acids, nicotinic acids, vitamins B1, vitamins B2, biotins folic acids, vitamins B12, etc. Water-soluble vitamin Cs, oil-soluble vitamin Cs, vitamin Ks; vitamin-like acting factors such as ferulic acid can be mentioned.

Examples of the organic acids include gluconic acid, aspartic acid, aminoethyl sulfonic acid, citric acid, glutamate, succinic acid, oxalic acid, fumaric acid, propionic acid, malic acid, salicylic acid, glycolic acid, phytic acid, tartaric acid, and acetic acid. , Lactic acid, and salts thereof. Examples of the salt include a salt of a mineral acid such as sulfuric acid, hydrochloric acid or phosphoric acid, a salt of an organic acid such as maleic acid or methanesulfonic acid, an alkali metal salt such as sodium or potassium, an alkaline earth metal salt, an ammonium salt and the like. Can be mentioned.

Examples of the moisturizing component include high molecular compounds such as sodium hyaluronate, heparin analog, sodium chondroitin sulfate, collagen, elastin, keratin, chitin, chitosan, and proteoglycan; amino acids such as glycine, aspartic acid, and arginine; sodium lactate, Natural moisturizing factors such as urea and sodium pyrrolidone carboxylate; lipids such as ceramide, cholesterol and phospholipids; plant extracts such as chamomile extract, hamamelis extract, cha extract and perilla extract, and polyhydric alcohols such as glycerin.

The polyhydric alcohol preferably has 2 to 10 carbon atoms, and for example, glycerin, diglycerin, triglycerin, propylene glycol, dipropylene glycol, 1,3-butanediol, ethylene glycol, diethylene glycol, isoprene glycol, 1 , 3-butylene glycol, sorbitol, xylitol, erythritol, mannitol, pentandiol, hexanediol, octanediol, decanediol, neopentyl glycol and the like.

Examples of the scrubbing agent include apricot kernel powder, almond shell powder, apricot kernel powder, sodium chloride granules, olive kernel powder, seawater dried product granules, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, and charcoal powder. , Cherries shell powder, polyethylene powder, silicic anhydride and the like.

Examples of the ultraviolet absorbing component include ethylhexyl methoxycinnamate, hexyl diethylaminohydroxybenzoylbenzoate, bisethylhexyloxyphenol methoxyphenyltriazine, ethylhexyltriazone, t-butylmethoxydibenzoylmethane, octocrylene, polysilicone-15, and methylene. Examples thereof include bisbenzotriazolyl tetramethylbutylphenol, octyl dimethoxybenzylidene dioxoimidazolidine propionate, and phenylbenzimidazole sulfonic acid.

Examples of the ultraviolet scattering component include inorganic compounds such as hydrous silicic acid, zinc silicate, cerium silicate, titanium silicate, zirconium oxide, cerium oxide, titanium oxide, iron oxide, zinc oxide, and silicic anhydride. Inorganic compounds coated with inorganic powders such as hydrous silicic acid, aluminum hydroxide, mica and talc, composited with resin powders such as polyamide, polyethylene, polyester, polystyrene and nylon, and silicone oil and aluminum fatty acid Examples thereof include those treated with salt or the like.

Examples of the astringent component include alum, zinc sulfate, aluminum chloride, zinc sulfonate, tannic acid and the like.

Examples of the peptide or a derivative thereof include keratin-degrading peptide, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin-degrading peptide, collagen-degrading peptide, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, and the like. Elastin-degrading peptide, conchiolin-degrading peptide, hydrolyzed conchiolin, silk proteolytic peptide, hydrolyzed silk, lauroyl hydrolyzed silk sodium, soybean proteolytic peptide, hydrolyzed soybean protein, wheat protein, wheat proteolytic peptide, hydrolyzed wheat protein, Examples thereof include casein-degrading peptides and acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.).

Examples of the amino acid or a derivative thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, and the like. Examples thereof include cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosin and creatine.

Examples of the cleaning component include soaps selected from alkali metal salts such as potassium laurate, potassium myristate, potassium palmitate or potassium stearate, alkanolamide salts or amino acid salts; Na cocoyl glutamate, Na cocoyl methyl taurine Amino acid-based surfactants such as; ether sulfate ester salts such as laureth sulfate Na; ether carboxylates such as lauryl ether acetate; sulfosuccinic acid ester salts such as alkyl sulfosuccinate Na; palm oil fatty acid monoethanolamide, palm oil Amino acid alkanolamides such as fatty acid diethanolamide; monoalkyl phosphate esters such as sodium lauryl phosphate and sodium polyoxyethylene lauryl ether phosphate; coconut oil fatty acid amide propyldimethylaminoacetic acid betaine, lauryldimethylaminoacetic acid betaine, 2-alkyl Betain-type amphoteric surfactants such as -N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, lauryl hydroxysulfobetaine and lauroylamide ethyl hydroxyethyl carboxymethyl betaine hydroxypropyl phosphate; amino acids such as sodium lauryl aminopropionate Examples include type amphoteric surfactants.

Examples of the keratin softening component include lactic acid, salicylic acid, glucolic acid, citric acid, malic acid, fruit acid, phytic acid, urea, and sulfur.

Examples of the cell activating component include amino acids such as γ-aminobutyric acid; vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids; α-hydroxy acids such as glycolic acid and lactic acid; tannins and flavonoids. Examples thereof include saponin and photosensitizer No. 301.

Examples of the blood circulation promoting action component include plants (for example, Otaneninjin, Ashitaba, Arnica, Ginkgo, Angelica acutiloba, Enmeisou, Dutch oak, Chamomile, Roman chamomile, Carrot, Gentiana, Gobo, Rice, Sanzashi, Shiitake, Grape, Seiyousanzashi, Seiyounezu. , Senkyu, Senburi, Thyme, Chouji, Chimpi, Togarashi, Touki, Tounin, Tohi, Carrot, Garlic, Butcher Bloom, Grape, Button, Maronie, Melissa, Yuzu, Yokuinin, Ryokucha, Rosemary, Rosehip, Chimpi, Touki, Ingredients derived from (Angelica acutiloba, peach, apricot, walnut, corn, etc.); acetylcholine, ictamol, cantalistinki, gamma oryzanol, cepharanthin, trazoline, tocopherol nicotinate, glucosyl hesperidine and the like.

[Manufacturing method of cosmetological composition]
The method for producing the cosmetological composition of the present invention is not particularly limited, and an IPL irradiation-like agent which is an essential component, other components to be blended as necessary (the above-mentioned optional component, a base or carrier described later, an additive, etc.) ) Can be appropriately selected and blended, and can be produced by a conventional method.

[Use of beauty composition]
The cosmetological composition of the present invention is an approach of a cosmetologist's idea of IPL treatment (light treatment), and is used with the expectation of an effect on the entire face similar to IPL irradiation. By using the beauty composition of the present invention, the effect of whitening the entire face and improving the skin tone can be obtained. It is also effective in improving aging symptoms (skin tone improvement) such as dullness of the entire face, lack of firmness, conspicuous pores, and dryness. As described above, the cosmetological composition of the present invention can be used as a substitute for IPL irradiation and in combination with IPL irradiation. The beauty composition of the present invention used for such a purpose includes, for example, beauty essence, lotion, milky lotion, cream, gel cream, beauty mask (face mask), makeup base, foundation, sunscreen, cleansing, cleaning agent and the like. It can be suitably used as an external composition of, and more preferably it can be used as a skin care product such as a beauty essence, a lotion, a milky lotion, a cream, and a gel cream.

In addition, the cosmetological composition of the present invention can be a food composition. This food composition can be suitably used as a health food, a dietary supplement (including a balanced nutritional diet, a supplement, etc.). It is also suitable for foods with health claims (foods for specified health use (including disease risk reduction labeling and standard type)).

[Formulation (external composition)]
When the cosmetological composition of the present invention is an external composition, the essential ingredients and other optional ingredients described above are used as a base or carrier usually used for cosmetics, pharmaceuticals, quasi-drugs, and necessary. Therefore, it can be mixed with an additive described later according to a conventional method and emulsified or solubilized as necessary to obtain a cosmetological composition in various formulation forms.

Examples of the base or carrier include hydrocarbons such as liquid paraffin, squalane, vaseline, gelled hydrocarbon (plastibase, etc.), ozokelite, α-olefin oligomer, light liquid paraffin; methylpolysiloxane, cyclic silicone, modified silicone. , Silicone oils such as silicone resins; oils and fats such as palm oil, olive oil, rice bran oil, shea butter; waxes such as jojoba oil, miuro, candelilla wax, lanolin; cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol, octyldodeca Higher alcohols such as ol, isostearyl alcohol, phytosterol, cholesterol; methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose polyvinylpyrrolidone, carrageenan, polyethylene glycol, isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, Examples thereof include esters such as isononyl isononanoate and pentaerythritol tetra2-ethylhexanoate; polysaccharides such as dextrin and maltodextrin; lower alcohols such as ethanol; glycol ethers such as diethylene glycol monoethyl ether; and water.

The base or carrier described above may be used alone or in combination of two or more. Moreover, the amount used thereof is appropriately selected from a range known to those skilled in the art.

[Additives (external composition)]
The cosmetological composition of the present invention includes known additives to be added to cosmetics, pharmaceuticals and quasi-drugs, for example, surfactants, antioxidants, colorants and pearls, as long as the effects of the present invention are not impaired. A gloss-imparting agent, a chelating agent, a pH adjuster, a preservative, a thickener, and the like can be added. These additives may be used alone or in combination of two or more.

The surfactant may be any of nonionic surfactants, cationic surfactants, anionic surfactants, amphoteric surfactants and the like, and for example, sorbitan monoisostearate and sorbitan monolaurate. , Polysorbate monopalmitate, sorbitan monostearate, sorbitan fatty acid esters such as penta-2-ethylhexylate diglycerol sorbitan, tetra-2-ethylhexylate diglycerol sorbitan; propylene glycol fatty acid esters such as propylene glycol monostearate; Hardened sardines such as polyoxyethylene cured sardine oil 40 (HCO-40), polyoxyethylene cured sardine oil 50 (HCO-50), polyoxyethylene cured sardine oil 60 (HCO-60), and polyoxyethylene cured sardine oil 80. Oil derivatives; polyoxyethylene monolaurate (20) sorbitan (polysorbate 20), polyoxyethylene monostearate (20) sorbitan (polysorbate 60), polyoxyethylene monooleate (20) sorbitan (polysorbate 80), isostearic acid Polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene (20) sorbitan; polyoxyethylene monococonut oil fatty acid glyceryl; glycerin alkyl ether; alkyl glucoside; polyoxyalkylene alkyl ether such as polyoxyethylene cetyl ether; stearylamine, oleylamine Amins such as: polyoxyethylene / methylpolysiloxane copolymer, lauryl PEG-9 polydimethylsiloxyethyl dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone and other silicone-based surfactants.

Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, sorbic acid, sodium sulfite, ascorbic acid, erythorbic acid, L-cysteine hydrochloride and the like.

Examples of the colorant include inorganic pigments and natural pigments.

Examples of the chelating agent include EDTA / disodium salt, hirodoxyetandiphosphonic acid / calcium / disodium salt and the like.

Examples of the pH adjuster include inorganic acids (hydrochloride, sulfuric acid, etc.), organic acids (lactic acid, sodium lactate, citrate, sodium citrate, succinate, sodium succinate, etc.), and inorganic bases (potassium hydroxide, hydroxide). (Sodium, etc.), organic bases (triethanolamine, diisopropanolamine, triisopropanolamine, etc.) and the like.

Examples of the preservative include benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, ethyl paraoxybenzoate, propyl paraoxybenzoate, methyl paraoxybenzoate, phenoxyethanol, caprylhydroxamic acid, pentanediol, hexanediol, octane. Examples thereof include alkanediols such as diols.

Examples of the thickener include vinyl thickeners such as polyvinyl alcohol, polyvinylpyrrolidone, and carboxyvinyl polymers, methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, and carboxyethyl cellulose. Cellulose thickeners, guar gum, pectin, pullulan, gelatin, locust bean gum, carrageenan, agar, xanthan gum, alkyl methacrylate copolymer, polyethylene glycol, bentonite, alginic acid, propylene glycol alginate, macrogol, chondroitin sulfate. Examples thereof include sodium, hyaluronic acid, sodium hyaluronate, (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer, (acryloyldimethyltaurinammonium / vinylpyrrolidone) copolymer and the like.

[Formulation form (external composition)]
When the beauty composition of the present invention is an external composition, the formulation form is not particularly limited, and for example, a liquid preparation (lotion, lotion, beauty essence, etc.), a cream preparation (milky lotion, cream, gel cream, balm, etc.), Examples include gel preparations, jelly preparations, sherbet preparations, aerosol preparations, foam preparations, spray preparations, patches, stick preparations, other ointments, solid preparations and the like. These preparations can be produced according to a conventional method, for example, the method described in the 17th revised Japanese Pharmacopoeia General Rules for Preparations. Further, the dosage form such as W / O, O / W, W / O / W, O / W / O type emulsion can be appropriately selected in consideration of the desired stability, usability and the like. The patch may be, for example, a non-woven fabric sheet, a gel sheet or the like impregnated with a beauty essence composition, a lotion composition, a milky lotion composition or the like.

[Formulation (food composition)]
When the beauty composition of the present invention is a food composition, the essential ingredients and other optional ingredients described above are added to a base or carrier usually used in foods, and if necessary, fats and oils, emulsifiers, etc. It may be mixed with food additives such as dispersants, suspending agents, stabilizers, thickeners, sweeteners, colorants, flavors, preservatives, antioxidants and organic acids to formulate them. The food additive is not limited to the above and the specific ingredients described later, and can be appropriately selected according to the purpose and necessity. In addition, these food additives can be used alone or in combination of two or more. In addition, other active ingredients can be added to the formulation.

Examples of the above fats and oils include natural vegetable oils such as peanut oil, cocoa butter, rice germ oil, perilla oil, and flaxseed oil, hydrogenated oils thereof, and glycerides (glycerides, diglycerides, triglycerides, etc.) of fatty acids (including medium-chain fatty acids). Mitsurou and the like can be mentioned. Of these, beeswax is preferable.

Examples of the emulsifier, dispersant, suspending agent and stabilizer include polyhydric alcohols such as polyethylene glycol, propylene glycol, glycerin and sorbitol; glycerin fatty acid ester; sodium carboxymethyl cellulose and the like. Of these, glycerin, glycerin fatty acid ester, and sodium carboxymethyl cellulose are preferable.

Examples of the thickener include locust bean gum, guar gum, xanthan gum, tamarind gum, pectin, carrageenan and the like. Of these, xanthan gum is preferred.

Examples of the sweetener include sucrose, fructose, maltose, erythritol, trehalose, maltitol, licorice extract, stevia-processed sweetener, aspartame, acesulfame potassium, sorbitol, xylitol and the like.

Examples of the preservative include benzoic acid or a salt thereof, sorbic acid or a salt thereof, butyl paraoxybenzoate, isobutyl paraoxybenzoate, isopropyl paraoxybenzoate, ethyl paraoxybenzoate, methyl paraoxybenzoate and the like.

Examples of the above-mentioned antioxidants include ascorbic acid, sodium ascorbic acid, ascorbic acid stearic acid ester, sodium ascorbic acid stearate, ascorbic acid palmitate, hydrogen sulfite, sodium hyposulfite, sulfur dioxide, EDTA calcium disodium, and erythorbic acid. , Sodium erythorbate, tocopherol and the like. Of these, ascorbic acid, ascorbic acid palmitic acid ester, and tocopherol are preferable.

Examples of the organic acid include citric acid, succinic acid, tartaric acid, aspargic acid, lactic acid, malic acid, malonic acid, fumaric acid, maleic acid and the like.

[PH]
The pH of the cosmetological composition of the present invention is usually pH 3.0 to 8.0, preferably pH 3.5 to 7.5. This pH can be adjusted, for example, by using a pH adjuster described later. However, this does not apply to formulations in which pH measurement is impossible or difficult.

<Screening method>
The present invention also includes a method for screening a component for improving skin tone and a method for screening a component for improving skin tone. The screening method of the present invention is a method characterized by using a gene whose expression is changed by irradiating skin cells with IPL as an index, and is a step (1) of allowing a test substance to act on cultured skin cells, the above-mentioned skin culture. At least the step (2) of measuring the gene expression in the cell is included. According to the screening method of the present invention, it is possible to efficiently obtain a skin tone improving component and a skin tone improving component that can obtain the same effect as when receiving IPL irradiation (light treatment).

Step (1): The skin culture cell is not particularly limited as long as it is a cell population containing melanocytes, but for example, epidermal cells, dermis cells, cultured skin model and the like can be used. As the cultured skin model, it is more preferable to use MEL-300A (MatTek) or EFT-400 (MatTek). The test substance is added to the skin culture cells and cultured for about 2 hours to 72 hours. As a negative control, one to which no test substance is added is prepared.

Step (2): After the above step (1), cells are collected, RNA is extracted according to a conventional method, and gene expression is analyzed by a DNA microarray method or the like. In step (1), the gene expression of the group to which the test substance was added and the group to which the test substance was not added (negative control) are matched. At this time, as the corresponding gene, a gene that has been confirmed to cause fluctuation by IPL irradiation of skin cells is used. Examples of such genes include OCA2, SLC45A2, RAB27A2, and HB-EGF. These genes are genes found by the present inventors that their expression is suppressed or promoted by IPL irradiation. In the screening method of the present invention, it is confirmed whether or not the expression of each gene fluctuates in the addition group of the test substance in the same manner as in the case of IPL irradiation. Then, the test substance that changes the expression of these genes as in the case of IPL irradiation can be determined to be a skin tone improving component and a skin tone improving component.

<Cosmetology method>
The present invention also includes a cosmetological method for improving / improving the skin condition, which comprises using an IPL irradiation-like agent. According to the beauty method of the present invention, by using the IPL irradiation-like agent, the same effect as IPL irradiation can be obtained, so that, for example, the effect of whitening the entire face and improving the skin tone can be obtained. In addition, an effect of improving aging symptoms (skin tone improving effect) such as dullness of the entire face, disorder of texture, fine wrinkles, and telangiectasia can be obtained. As for the specific description of the IPL irradiation-like agent, the description in the section of the above-mentioned beauty composition can be applied as it is.

<IPL irradiation-like agent>
The present invention also includes an IPL irradiation-like agent containing various plant extracts as active ingredients. As the above-mentioned plant extract, a plant extract containing flavonoids and a seaweed extract are preferable. In addition, as plant extracts containing the above flavonoids, Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, Melia azalea lacta leaf extract, Mandarin orange peel extract, Yukinoshita extract, Seiyo obaco seed At least one plant extract selected from the group consisting of extracts, kudamonotokeisou fruit extracts, hop flower extracts, tambrissa trichophylla leaf extracts, rosemary extracts, artea extracts and gobo extracts is more preferred. Examples of the seaweed extract include brown algae (Gagomekonbu, Risilimb, Mitsuishikonbu, Macombu, Nagakombu, Wakame seaweed, etc.), Red algae (Gelidiaceae, etc.), Green algae (Aonori, etc.), Blue algae (Spirulina, etc.), and more. preferable.

That is, as the IPL irradiation-like agent of the present invention, Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, Melia azalea lacta leaf extract, Mandarin orange peel extract, Yukinoshita extract, Seiyoobako seed It is preferable that the active ingredient contains at least one plant extract selected from the group consisting of extracts, kudamonotokeisou fruit extracts, hop flower extracts and tambrissa trichophylla leaf extracts, rosemary extracts, gobo extracts, and artea extracts. In addition, the IPL irradiation-like agent of the present invention preferably contains at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue-green algae as an active ingredient, and the seaweed extract of brown algae is preferable. Those contained as an active ingredient are more preferable.

For details of the plant extract and seaweed extract contained in the IPL irradiation-like agent of the present invention as an active ingredient, the description of the IPL irradiation-like agent in the above-mentioned section on beauty composition can be applied.

According to the IPL irradiation-like agent of the present invention, the same effect as IPL irradiation can be obtained. Therefore, when a beauty composition containing this is used, for example, whitening of the whole face, improvement effect of skin tone, dullness of the whole face, etc. An effect of improving aging symptoms (improving skin tone) such as lack of firmness, conspicuous pores, and dryness can be obtained. As for the specific description of the IPL irradiation-like agent, the description in the section of the above-mentioned beauty composition can be applied as it is.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.

[Test 1] Analysis of changes in epidermal gene expression by IPL irradiation The following tests were performed using an epidermal model containing human melanocytes (MatTek; MEL-300A). That is, after arriving at MEL-300A, the cells are acclimatized at 37 ° C. in an incubator whose CO ₂ concentration is set to 5%, and culture is started in a liquid medium (Kurabo Industries: EPI-100LLMM). After completion of the acclimation culture, the epidermis sheet in the transwell was cut out, placed on gauze containing a liquid medium, and irradiated under the conditions according to the actual treatment. The epidermis model prepared by the above method was irradiated with light (IPL560 nm) using a filter of λ560 nm for 3 ms, then irradiated for another 3 ms at 20 ms, and cells were collected after 6 hours and 24 hours. .. The IPL irradiation was performed using Photofacial M22 (manufactured by Luminous). RNA was extracted from each cell according to a conventional method, and gene expression was analyzed by the DNA microarray method. For the DNA microarray, Human Gene 2.0ST Array was used.

OCA2, RAB27A, SLC45A2, and HB-EGF were selected as genes whose expression was changed in epidermal cells by irradiation with IPL 560 nm.

[Test 2] Screening of substances that affect the expression of OCA2, SLC45A2, and RAB27A genes in human melanocytes Normal human adult epidermal melanin cells (Kurabo: NHEM) were used as a medium for melanocytes (Kurabo: DermaLife Ma). Then, the cells were seeded on a 24-well plate (CORNING) at 40,000 cells / well and cultured for 2 days. Then, each extract sample was prepared in a medium so as to have each concentration, passed through a filter having a pore size of 0.2 μm, added to a 24-well plate from which the medium had been removed at 400 μL / well, and further cultured for 24 hours. The cells were collected. RNA was extracted by a conventional method, cDNA was synthesized from RNA by a reverse transcription reaction, and the expression levels of each gene of OCA2, SLC45A2, and RAB27A were quantified by quantitative real-time PCR. The obtained value was used to calculate the ratio to the blank. The blank refers to the data of the control test using only the medium to which each extract sample is not added. The results are shown in Tables 1 to 17 below.

[Test 3] Screening of substances that affect the expression of the HB-EGF gene in human keratinocytes Normal human adult epidermal cells (Kurabo: NHEK) were used in a medium for epidermal keratinocytes (Kurabo: Humania KG2). , 24-well plates (CORNING) were seeded at 40,000 cells / well and cultured for 1 day. Then, each extract sample was prepared in a medium so as to have each concentration, passed through a filter having a pore size of 0.2 μm, added to a 24-well plate from which the medium had been removed at 400 μL / well, and further cultured for 24 hours. The cells were collected. RNA was extracted by a conventional method, cDNA was synthesized from RNA by a reverse transcription reaction, and the expression level of each gene of HB-EGF was quantified by quantitative real-time PCR. The obtained value was used to calculate the ratio to the blank. The blank refers to the data of the control test using only the medium to which each extract sample is not added. The results are shown in Table 18 below.

From the above, the beauty composition and the beauty method of the present invention can be expected to have an effect of brightening the skin having a darker skin tone (skin tone improving effect), and the beauty composition of the present invention can be used as a skin tone improving agent. Can also be used. In addition, the beauty composition and beauty method of the present invention can be expected to have an effect of improving aging symptoms (skin tone improving effect) such as dullness of the entire face, lack of firmness, conspicuous pores, and dryness, and are also used as a skin tone improving agent. it can.

An example of prescribing the cosmetological composition of the present invention is shown below.

Prescription example 1
(Essence)
L-ascorbic acid 10.0 (w / w%)
Hydroquinone 0.1
Diethylene glycol monoethyl ether 51.0
Propylene glycol 23.0
Jiaogulan extract 0.1
Neubara fruit extract 0.5
Total remaining amount of purified water 100.0%

Prescription example 2
(Essence)
3-O-ethylascorbic acid 3.0 (w / w%)
Diethylene glycol monoethyl ether 30.0
Propylene glycol 20.0
Glycerin 4.0
Artea Extract 1.0
Artichoke extract 10.0
Total remaining amount of purified water 100.0%

Prescription example 3
(Toner)
Tranexamic acid 1.5 (w / w%)
Salicylic acid 0.1
Sodium hyaluronate 0.2
Glycerin 2.0
1,3-butylene glycol 10.0
Polyoxypropylene methyl glucoside 0.2
Acetylated sodium hyaluronate 0.01
Hydroxyethyl cellulose 0.05
Methyl paraoxybenzoate 0.15
Fragrance 0.03
Oil-soluble licorice extract 0.0001
Total remaining amount of purified water 100.0%

Prescription example 4
(Toner)
Tranexamic acid 1.5 (w / w%)
Salicylic acid 0.1
Sodium hyaluronate 0.2
Glycerin 1.0
Diglycerin 0.5
1,3-butylene glycol 5.0
Dipropylene glycol 3.0
Trehalose 0.1
Acetylated sodium hyaluronate 0.01
Hydroxyethyl cellulose 0.05
Methyl paraoxybenzoate 0.15
Fragrance 0.03
Seaweed extract 0.5
Rosemary extract 0.3
Total remaining amount of purified water 100.0%

Prescription example 5 (toner)
L-ascorbyl magnesium phosphate 3.0 (w / w%)
Placenta extract 0.5
Trimethylglycine 3.0
Hyaluronic acid 0.05
Gagomekonbu extract 0.3
1,3-butylene glycol 5.0
Polyoxyethylene cured castor oil 1.0
Methyl paraoxybenzoate 0.05
White willow bark extract 20.0
Licorice root extract 0.01
Total remaining amount of purified water 100.0%

Prescription example 6 (milky lotion)
Tranexamic acid 2.0 (w / w%)
β-Arbutin 0.1
Salicylic acid 0.2
Sodium hyaluronate 0.2
Dipropylene glycol 5.0
1,3-butylene glycol 8.0
Glycerin 3.0
Glycerin monostearate 1.0
Carboxyvinyl polymer 0.2
Xanthan gum 0.05
Meadowfoam oil 5.0
Glyceryl tri2-ethylhexanoate 5.0
Methylpolysiloxane 1.0
Triethanolamine 0.1
Tocopherol 0.1
Acetylated sodium hyaluronate 0.05
Sodium edetate 0.05
Methyl paraoxybenzoate 0.2
Fragrance 0.05
Rosemary extract 0.1
Agetsu extract 0.1
Total remaining amount of purified water 100.0%

Prescription example 7 (cream)
Nicotinamide 1.0 (w / w%)
Glycerin 5.0
1,3-butylene glycol 5.0
Light liquid paraffin 3.0
Alkylate Alkylate Copolymer Acrylic Acid 0.3
Arginine 0.3
Polyoxyethylene hardened castor oil 0.5
Methyl paraoxybenzoate 0.2
Melia bruises leaf extract 0.5
Mandarin orange peel extract 3.0
Purified water Appropriate amount total 100.0%

Prescription example 8 (cream)
Tranexamic acid 1.0 (w / w%)
Arbutin 3.0
Salicylic acid 0.05
Sodium hyaluronate 0.15
Dipropylene glycol 8.0
Diglycerin 5.0
Glycerin 3.0
Hydroxyethylurea 3.0
Polyoxyethylene sorbitan isostearate 0.5
Glycerin monostearate 0.4
Setostearyl glucoside / setostearyl alcohol 1.5
Acrylic acid / alkyl methacrylate copolymer 0.5
Meadowfoam oil 0.5
Squalene 3.0
Tri (caprylic acid / capric acid) glyceryl 1.5
Methylpolysiloxane 1.0
Behenyl alcohol 0.2
Stearyl alcohol 0.3
Xanthan gum 0.2
Sodium hydroxide 0.1
Sodium edetate 0.1
Methyl paraoxybenzoate 0.2
Fragrance 0.1
Ceramide 1 0.01
Ceramide 2 0.01
Ceramide 6 0.01
Rosemary extract 0.001
Yukinoshita extract 0.01
Tambrissa trichophylla leaf extract 0.001
Agetsu Extract 5.0
Total remaining amount of purified water 100.0%

Prescription example 9 (essence)
Nicotinamide 5.0 (w / w%)
Glycerin 2.0
1,3-butylene glycol 3.0
Squalan 2.0
Triethylhexanoin 1.0
Carboxyvinyl polymer 0.2
(Acryloyl dimethyl taurine ammonium / vinylpyrrolidone)
Copolymer 0.2
Polyoxyethylene Polyoxypropylene Butyl Ether (17EO) (17PO) 0.5
Polyoxypropylene methyl glucoside 0.5
Triethanolamine 0.15
Hydrogenated soybean phospholipid 0.5
Methyl paraoxybenzoate 0.2
Blueberry leaf extract 0.05
Comfrey leaf extract 0.05
Purified water Appropriate amount total 100.0%

Prescription example 10 (essence)
Nicotinamide 5.0 (w / w%)
Dipropylene glycol 5.0
1,3-butylene glycol 5.0
Light liquid paraffin 2.0
Methylphenyl polysiloxane 1.0
Xanthan gum 0.5
(PEG-240 / decyltetradeceth-20 / HDI) copolymer
1.5
Cholesteryl oleate 0.2
Polyoxyethylene hardened castor oil 0.5
Hydrogenated soybean phospholipid 0.2
Pentanediol 1.0
Phenoxyethanol 0.2
Macademia nut fatty acid phytosteryl 0.1
Himefuuro extract 0.05
Licorice root extract 0.05
Purified water Appropriate amount total 100.0%

Prescription example 11 (cream)
Nicotinamide 5.0 (w / w%)
Glycerin 5.0
1,3-butylene glycol 10.0
1,2-Pentanediol 0.5
Vegetable squalane 5.0
Tri (capryl capric acid) glyceryl 1.0
Methylpolysiloxane 1.0
Carboxyvinyl polymer 0.4
Xanthan gum 0.05
Polyoxyethylene sorbitan monostearate 1.5
Sorbitan monostearate 0.5
Polyoxyethylene cetyl ether 0.2
Glycerin mono2-ethylhexyl ether 0.5
Ceramide 2 0.0005
Ceramide 6II 0.00005
Seaweed extract (1) 2.0%
Artea Extract 0.01
pH adjuster Appropriate amount Purified water Appropriate amount Total 100.0%

Prescription example 12 (sunscreen)
Ascorbyl tetra2-hexyldecanoate 0.1 (w / w%)
Trimethylglycine 1.0
2-Ethylhexyl paramethoxycinnamate 10.0
Decamethylcyclopentasiloxane 20.0
Octyl palmitate 10.0
Polyoxyethylene / methylpolysiloxane copolymer 3.0
Methyl hydrogen polysiloxane treated low temperature calcined zinc oxide 15.0
Alkylate polyacrylate 5.0
Glycerin 5.0
Absolute ethanol 5.0
1,3-butylene glycol 3.0
Panthenol 0.1
Fragrance 0.1
Ceramide 2 0.001
Ceramide 3 0.001
Ceramide 6 0.001
Broadleaf plantain seed extract 0.01
Passionflower fruit extract 0.1
Total remaining amount of purified water 100.0%

Prescription example 13 (cleansing)
L-ascorbic acid 2-glucoside 0.1 (w / w%)
PEG-20 glyceryl triisostearate 12.0
Polyglyceryl isostearate-10 8.0
Ethylhexyl palmitate 30.0
Triethylhexanoin 20.0
Mineral oil 25.0
Diphenylsiloxyphenyltrimethicone 1.0
Hop flower extract 0.0001
Total remaining amount of purified water 100.0%

Prescription example 14 (cleansing)
L-ascorbic acid 2-glucoside 0.1 (w / w%)
PEG-20 glyceryl triisostearate 12.0
Polyglyceryl isostearate-10 8.0
Ethylhexyl palmitate 28.0
Triethylhexanoin 20.0
Mineral oil 20.0
Diphenylsiloxyphenyltrimethicone 1.0
Seaweed extract 0.0001
Artea Extract 0.0001
Total remaining amount of purified water 100.0%

Prescription Example 15 (Soft Capsule: Food Composition)
The unit of numerical value in the formulation examples of the following food compositions is "part by weight".
Capsule contents Evening primrose oil 120
Hydrolyzable tannins 20
Hydrolyzed soy protein 20
Lotus germ extract 5
Ceramide 3
Hyaluronic acid 0.5
CoQ10 2
Haematococcus algae pigment extract (containing astaxanthin) 4
Vitamin C 1
Glycerin fatty acid ester 10
Beeswax 5
Capsule film gelatin 92
Pullulan 3
Glycerin 35
Coloring agent 3

Prescription example 16 (beauty drink) unit (mg)
Licorice extract 180
Placenta extract 180
Collagen peptide 1000
Artichoke extract 50
Proteoglycan 20
Jiaogulan Extract 20
Polysorbate 80 0.1
Erythritol 3
Agar 0.5
Syrup 0.6
Fructose 2
Acidulant 0.5
Fragrance 0.1
Water 86.1
Total daily intake (mg) 1542.9

Prescription example 17 (beauty drink) unit (mg)
Seaweed extract 180
Placenta extract 180
Collagen peptide 1000
Proteoglycan 20
Rosemary extract 40
Polysorbate 80 0.1
Erythritol 3
Agar 0.5
Syrup 0.6
Fructose 2
Acidulant 0.5
Fragrance 0.1
Water 103.2
Total daily intake (mg) 1530.0

Claims (14)

A cosmetological composition containing an IPL irradiation-like agent. The cosmetological composition according to claim 1, wherein the IPL irradiation-like agent contains at least a plant extract containing flavonoids as an active ingredient. The above plant extracts are: Uridae, Asteraceae, Asteraceae, Rosaceae, Salixaceae, Sendan, Rutaceae, Saxifragaceae, Plantains, Asteraceae, Asa, Monimia, Tochinoki, Ericaceae, Buttonaceae. The beauty composition according to claim 2, which is an extract of at least one plant selected from the group consisting of Rutaceae, Plantainaceae, and Saxifragaceae. The cosmetological composition according to claim 1, wherein the IPL irradiation-like agent contains at least a seaweed extract as an active ingredient. The cosmetological composition according to any one of claims 1 to 4, wherein the IPL irradiation-like agent regulates the expression of melanin-related molecules in skin cells. The cosmetological composition according to claim 5, wherein the melanin-related molecule is at least one selected from the group consisting of OCA2, SLC45A2, RAB27A2, and HB-EGF. The above plant extracts are Amachazuru extract, Artichoke extract, Kanzo root extract, Neubara fruit extract, Shiroyanagi bark extract, Melia azajirakuta leaf extract, Mandarin orange bark extract, Yukinoshita extract, Oobako seed extract, Kudamonotokeisou fruit extract, The invention according to any one of claims 2, 3, 5, and 6, which is at least one selected from the group consisting of hop flower extract, tambrissa trichophylla leaf extract, rosemary extract, gobo extract, and artea extract. Beauty composition. Further, according to any one of claims 1 to 7, it contains at least one selected from the group consisting of a turnover accelerator (excluding the above-mentioned IPL irradiation-like agent), an anti-glycation component and a whitening active ingredient. Beauty composition. The cosmetological composition according to any one of claims 1 to 8, which is for a skin tone improver. A method for screening a component for improving skin tone, which comprises screening a component effective for improving skin tone using a gene whose expression is changed by irradiating skin cells with IPL as an index. The screening method according to claim 10, further comprising a step of allowing a test substance to act on cultured skin cells and a step of measuring the gene expression in the cultured skin cells. A cosmetological method for improving / improving skin condition, which comprises using an IPL irradiation-like agent. Amachazuru extract, artichoke extract, kanzo root extract, sardine fruit extract, sardine bark extract, melia azalea leaf extract, mandarin orange bark extract, yukinoshita extract, sardine seed extract, kudamonotokeisou fruit extract, hop flower extract and tambrissa An IPL irradiation-like agent containing at least one plant extract selected from the group consisting of trichophylla leaf extract, rosemary extract, gobo extract, and artea extract as an active ingredient. An IPL irradiation-like agent containing at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue-green algae as an active ingredient.