WO2019009472A1 - Marqueur permettant la détermination de la présence d'arachis hypogaea dans des aliments et son utilisation - Google Patents

Marqueur permettant la détermination de la présence d'arachis hypogaea dans des aliments et son utilisation Download PDF

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WO2019009472A1
WO2019009472A1 PCT/KR2017/013320 KR2017013320W WO2019009472A1 WO 2019009472 A1 WO2019009472 A1 WO 2019009472A1 KR 2017013320 W KR2017013320 W KR 2017013320W WO 2019009472 A1 WO2019009472 A1 WO 2019009472A1
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antibody
peanut
food
present
peanuts
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PCT/KR2017/013320
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English (en)
Korean (ko)
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심원보
김솔아
이정은
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경상대학교산학협력단
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the present invention relates to a marker for discriminating whether or not a peanut is incorporated in a food and a use thereof.
  • Food allergy is a disease caused by immunoglobulin E (IgE) -mediated or non-immunoglobulin-mediated hypersensitivity to dietary protein, as well as digestive organs, skin, respiratory or cardiovascular Is a relatively common allergic disease that manifests various symptoms. It is a harmless food for normal people, but it refers to the excessive immune response of the human body to a specific protein of the food when it is ingested by a specific person. The incidence of food allergy is reported to be about 8% in children younger than 3 years, and about 2% in adults.
  • IgE immunoglobulin E
  • the amount of food a person consumes for a lifetime is about 2 to 3 tons and is exposed to a wide variety of foods, but the human gastrointestinal immune system has evolved to cause immunological tolerance to most dietary proteins, For very limited types of food, sensitization to dietary proteins occurs and ultimately allergic symptoms are induced.
  • Food allergens are defined as components of foods that induce the production of immunoglobulin E and cause an immediate hypersensitivity reaction, as well as inhalation and contact food allergens. Most major allergens in food are water-soluble glycoproteins with a molecular weight of about 10,000-60,000 Da. Unlike inhalational allergens, they are likely to be transformed by digestive enzymes, bacteria, toxins, etc. during their passage through the gastrointestinal tract, Anaphylaxis reactions as well as delayed type reactions (delayed type reaction) are often caused. Common foods that cause allergic reactions by immunoglobulin E include eggs, milk, beans, peanuts, nuts, buckwheat, wheat, fish, and shellfish.
  • the peanut Arachis hypogaea
  • the peanut is one of the food allergens that can cause severe systemic symptoms and lethal anaphylaxis. Unlike other foods, the natural loss does not tend to last for a lifetime.
  • a hybridoma cell capable of producing a monoclonal antibody capable of specifically binding to a peanut thermostable water-soluble protein Ara h1 was prepared, and a monoclonal antibody produced from the hybridoma cell was used for heat treatment It was confirmed that it is possible to quickly analyze the presence of peanuts in the mixture of food raw materials and processed foods.
  • Japanese Patent No. 3853692 discloses an antibody for testing peanut components, an assay method and an assay kit, and Korean Patent No. 1223256 discloses a method for reducing allergenicity of peanut and a peanut with reduced allergenicity.
  • the marker for discriminating the presence or absence of peanuts in the food of the present invention and the use thereof are not described.
  • the present invention has been made in view of the above-described needs, and it is an object of the present invention to provide a hive producing a monoclonal antibody which specifically binds to a thermostable water-soluble protein of a peanut using a heat- Lidomaju was prepared.
  • Immunoassay using the monoclonal antibody of the present invention showed that the presence or absence of peanut contamination can be quickly determined by the monoclonal antibody of the present invention regardless of whether or not heat treatment is applied to all food samples containing peanut
  • the present invention has been completed.
  • the present invention provides a marker for discriminating the presence or absence of peanuts in a food containing a peanut-derived Ara h1.
  • the present invention also provides a composition for determining the presence or absence of peanut in a food comprising an antibody that specifically binds to peanut-derived Ara h1.
  • the present invention provides a kit for judging whether or not a peanut is contained in a food comprising an antibody specifically binding to a pean-derived Ara h1.
  • the present invention provides a method for determining whether a peanut is incorporated in a food, comprising the step of detecting an antigen-antibody complex formed by contacting an antibody that specifically binds to peanut-derived Ara h1 with a test sample.
  • the present invention provides a monoclonal antibody produced by a hybridoma cell line of deposit number KCTC 13295BP and specifically binding to peanut-derived Ara h1.
  • the present invention provides a hybridoma cell line having the accession number KCTC 13295BP.
  • the monoclonal antibody of the present invention reacts specifically with Ara h1 among heat stable water soluble proteins in peanuts, and can effectively analyze peanuts incorporated in intentional unheated foods manufactured and processed by heat treatment. Further, through the present invention, information on the presence or absence of peanuts in foods can be provided to consumers to prevent food allergy induction, and it can be utilized to improve food manufacturing and processing, thereby contributing to securing food stability It is expected.
  • Figure 1 shows the results of SDS-PAGE of heat-stable proteins extracted from non-heat and heat-treated peanuts.
  • Lane 1 size marker
  • lane 2 green peanut sample extracted by non-heat treatment method
  • lane 3 green peanut sample extracted by heat treatment
  • lane 4 boiled peanut sample extracted by non-heat treatment method
  • lane 5 boiled peanut sample extracted by heat treatment
  • Lane 6 peanut samples extracted by non-heat treatment method
  • lane 7 roasted peanut samples extracted by heat treatment
  • lane 8 peanut samples extracted by non-heat treatment method
  • lane 9 peanut samples extracted by heat treatment.
  • FIG. 2 is a result of indirect ELISA measurement of anti-serum antibody levels of mice immunized with a heat-stable water-soluble protein extracted from roasted peanut and boiled peanut.
  • FIG. 3 is a graph showing the distribution of peanut (lane 2), boiled peanut (lane 3), peanut butter (lane 4), walnut (lane 5), almond (A and B) and ELISA (C) analysis of the heat-stable proteins extracted from soybean (lane 6), cashew nut (lane 7), red bean (lane 8) and soybean (lane 9).
  • Lane 1 Size marker.
  • Fig. 4 is a Western blot result for confirming the reaction of BP 3A1-12, a monoclonal antibody against Ara h1, a heat-stable protein in peanuts.
  • Lane 1 Size marker
  • Lane 2 Commercial Ara h1 protein
  • Lane 3 Roasted peanuts
  • Lane 4 Boiled peanuts
  • Lane 5 Peanut butter.
  • FIG. 5 shows the results of indirect ELISA measurement of the presence of peanuts in various processed foods using the antibody of the present invention.
  • Original Hybridoma cell culture, 1/10 diluted: Hybridoma cell culture diluted to 1/10.
  • 1/100 diluted Hybridoma cell culture diluted to 1/100.
  • A BP 3A1-12 antibody,
  • B RP 4C12-10 antibody.
  • FIG. 6 shows the results of measurement of the detection of the peptone protein content of the monoclonal antibodies (BP 3A1-12 and RP 4C12-10) of the present invention by indirect ELISA.
  • the present invention provides a marker for determining the presence or absence of a peanut in a food containing ara h1 from a peanut ( Arachis hypogaea ).
  • the Ara h1 is a seed storage protein or vicilin and is one of the peanut allergens.
  • Ara h1 which is resistant to heat and digestive enzymes, forms a stable trimmer structure due to the interaction between monomers, and there is an epitope (antigenic determinant) capable of binding immunoglobulin E at the contact site do.
  • an epitope antigenic determinant
  • heat treatment is applied to Ara h1
  • the immunoglobulin E binding epitope buried in the three-dimensional structure due to the protein modification is exposed, and the exposed epitope binds with immunoglobulin E to induce an allergic reaction.
  • the peanut-derived Ara h1 protein is detected in the food, it can be judged that the peanut is incorporated.
  • the marker may be used in the same manner as the marker composition for determining whether peanuts are mixed in the food.
  • the present invention also provides a composition for determining the presence or absence of peanut in a food comprising an antibody that specifically binds to peanut-derived Ara h1.
  • the composition includes an antibody that specifically binds to Ara h1 derived from peanuts as an active ingredient, and can determine the presence or absence of peanuts in the food using the antibody.
  • antibody is meant to include all forms as long as it specifically binds to a marker for discriminating the presence or absence of peanuts in the food of the present invention.
  • a monoclonal antibody polyclonal antibody, multispecific antibody (i.e., an antibody recognizing two or more antigens or two or more epitopes, such as a bispecific antibody, etc.)
  • Recombinant antibodies chemically modified antibodies that have the ability to specifically bind to a recognition marker.
  • fragments of the antibody include Fab, F (ab ') 2, scFv (an antibody in which a heavy or light chain Fv is linked by a suitable linker), Fv, Fab / c Means an antibody fragment obtained by introducing and expressing a gene for an antibody fragment or fragment obtained by treating with a protein cleaving enzyme such as papain or pepsin into a host cell by a gene recombination method.
  • the globulin type of the antibody may be any one of IgG, IgM, IgA, IgE, and IgD, provided that the globulin type of the antibody specifically binds to the marker for discriminating the presence or absence of peanuts in the food of the present invention.
  • the monoclonal antibody may preferably be produced by a hybridoma cell line having the accession number KCTC 13295BP, but is not limited to this.
  • the food may include, or is not limited to, all foods that contain or are expected to contain peanuts, preferably processed products such as peanuts, roasted peanuts, boiled peanuts, fried peanuts or butter peanuts.
  • the butter peanut refers to a product processed like a jam that is generally commercially available in the United States.
  • the present invention provides a kit for judging whether or not a peanut is contained in a food comprising an antibody specifically binding to a pean-derived Ara h1.
  • the antibody may include any type of antibody that specifically binds to the Ara h1 from the peanut as described above.
  • the antibody may be a monoclonal antibody that specifically binds to the Ara h1 from the peanut. More preferably, May be a monoclonal antibody produced by a hybridoma cell line with the accession number KCTC 13295BP, but is not now limited.
  • Kit systems for use with the present invention include, but are not limited to, ELISA plates, dip-stick devices, immunochromatography methods and split-split immune assay devices, flow-through devices, and the like.
  • the kit of the present invention is not particularly limited as long as it is used in a kit, but if it is an ELISA, for example, it may be a solid-phase supporter; A monoclonal antibody of the present invention; And an enzyme-linked immunosorbent assay (ELISA) reaction solution containing an enzyme-labeled antibody solution for reaction with an antigen and a coloring solution indicating an enzyme reaction.
  • ELISA enzyme-linked immunosorbent assay
  • the present invention provides a method for determining whether a peanut is incorporated in a food, comprising the step of detecting an antigen-antibody complex formed by contacting an antibody that specifically binds to peanut-derived Ara h1 with a test sample.
  • antigen-antibody complex in the present invention refers to a conjugate of Ara h1, a peanut thermostable water-soluble protein in a sample, and a monoclonal antibody according to the present invention, ),
  • An electrochemical method, a fluorimetric method, a luminometry method, a particle counting method, a visual assessment method, and a scintillation counting method It can be detected by any method. However, various applications and applications are possible without being limited thereto.
  • markers can be used for detecting an antigen-antibody complex.
  • Specific examples include, but are not limited to, enzymes, chromophores, ligands, luminescent materials, microparticles, and radioactive isotopes.
  • Examples of preferred enzymes used as the detection label include acetylcholinesterase, alkaline phosphatase,? -D-galactosidase, horseradish peroxidase or? -Lactamase, and preferable examples of the minerals include quantum dots, Eu 3+ , Eu 3+ chelate, or cryptate.
  • Preferred ligands include biotin derivatives.
  • Preferred emitters are acridinium esters or isoluminol derivatives.
  • Preferred microparticles include colloidal gold or There are colored latexes and preferred radioisotopes include, but are not limited to, 7 Co, 3 H, 125 I or 125 I Bolton Hunter reagents.
  • the method for detecting the antigen-antibody complex can be preferably detected using enzyme immunoassay (ELISA), but is not limited thereto.
  • Enzyme immunosorbent assays include direct ELISA using an antibody recognizing an antigen attached to a solid support, indirect ELISA using a labeled secondary antibody recognizing the capture antibody in a complex of antibodies recognizing an antigen attached to a solid support, attachment to a solid support
  • indirect sandwich ELISA using secondary antibodies include direct sandwich ELISA using an antibody recognizing an antigen attached to a solid support, indirect ELISA using a labeled secondary antibody recognizing the capture antibody in a complex of antibodies recognizing an antigen attached to a solid support, attachment to a solid support
  • the antibody may have a detection label, and when it does not have a detection label, the antibody can be captured and can be confirmed by treating another antibody having the detection label.
  • the present invention provides a monoclonal antibody that is produced by a hybridoma cell line with accession number KCTC 13295BP and specifically binds to pean-derived Ara h1.
  • monoclonal antibody means a highly specific antibody that is indicated in the art as a single antigenic site.
  • Monoclonal antibodies may also be referred to as monoclonal antibody, monoclonal antibody or monoclonal antibody.
  • monoclonal antibodies are directed against a single determinant on the antigen.
  • Monoclonal antibodies have the advantage of improving the selectivity and specificity of diagnostic and analytical assays that utilize antigen-antibody binding and also have other advantages that are not contaminated by other immunoglobulins because they are produced by hybridoma cultures.
  • the monoclonal antibody produced by the hybridoma may be used without purification. However, in order to obtain the best results, the monoclonal antibody produced by the hybridoma may be purified to a high purity (for example, 95% or more) according to a method well known in the technical field of the present invention . Such a purification technique can be separated from the culture medium or the plural liquids using a purification method such as gel electrophoresis, dialysis, salt precipitation, ion exchange chromatography, affinity chromatography and the like.
  • a purification method such as gel electrophoresis, dialysis, salt precipitation, ion exchange chromatography, affinity chromatography and the like.
  • the monoclonal antibody of the present invention was prepared from the hybridoma cell line BP 3A1-12, KCTC 13295BP deposited on Jul. 04, 2017 at the BRC.
  • the antibody of the present invention comprises (1) mixing peanuts with 0.5 M sodium chloride in a weight ratio of 1: 2; (2) reacting the peanut mixed with sodium chloride in boiling water for 15 minutes to extract a thermostable water-soluble protein from the peanut; (3) dialyzing the extracted thermostable water-soluble protein into a phosphate buffer, and injecting it into a mouse; (4) forming an antibody in the mouse against the injected thermostable water-soluble protein; (5) developing a hybridoma cell for monoclonal antibody production using spleen cells of a mouse in which an antibody is formed.
  • the method for producing a monoclonal antibody specific to a water-soluble protein of the peanut is disclosed.
  • peanut was mixed with 0.5 M sodium chloride at a weight ratio of 1: 2 and boiled for 15 minutes to extract a thermostable water-soluble protein.
  • the immunogen was injected into a mouse, and a spleen cell of an immunized mouse showing a high antibody titer was fused to a mouse-derived SP2 / 0 myeloma cell, which is a cancer cell, to prepare a hybridoma cell line.
  • the monoclonal antibody of the present invention was finally selected from the hybridoma cell line BP 3A1-12 and confirmed to be a monoclonal antibody that specifically binds to Ara h1 from peanuts.
  • the monoclonal antibody of the present invention was identified as BP 3A1-12 Respectively.
  • the present invention provides a hybridoma cell line wherein the accession number for producing said monoclonal antibody is KCTC 13295BP.
  • the hybridoma cell line of the present invention was deposited at KCTC 13295BP on Jul. 04, 2017 at the BRC, Korea Research Institute of Bioscience and Biotechnology.
  • the hybridoma cell line is a cell formed by fusion of an antibody-producing cell and a mouse-derived myeloma cell, and the hybridoma cell can continuously supply the antibody of the present invention.
  • thermostable soluble protein extracted from peanuts was used as an immunogen. Specifically, a 0.5 M sodium chloride (NaCl) solution was added to finely ground soybeans The mixture was cooled to room temperature and centrifuged at 3,220 x g for 15 min at 4 ° C. Then, the supernatant was collected by using Whatman No. 1. 1 filter paper, and the protein was extracted by dialyzing for 3 days while changing the phosphate buffered saline (PBS, pH 7.4) solution. The dialyzed protein crude extract was used as an immunogen. Protein concentrations of crude extracts were determined using a Quick start TM Bradford protein assay dye (Bio-Rad Laboratories Inc., USA).
  • the protein crude extract (100 ⁇ g / 100 ⁇ l PBS) was emulsified with the same amount (100 ⁇ l) of Freund's complete adjuvant, and the emulsified immunoglobulin (200 ⁇ l / mouse) was intraperitoneally injected into 7-week old BALB / . Subsequently, the protein crude extract was emulsified with an incomplete Freund ' s incomplete adjuvant at 2-week intervals, followed by second and third injection, and fourth injection of protein crude extract (200 ⁇ g / 200 ⁇ l PBS) . After the fourth injection, serum was collected from caudal vein and high antibody titers were confirmed by ELISA.
  • the culture supernatant of the hybridomas was measured by an indirect enzyme linked immunosorbent assay (ID-ELISA) using a plate coated with peanut antigens to determine the absorbance of the monoclonal antibody , MAb).
  • ID-ELISA indirect enzyme linked immunosorbent assay
  • Crude protein of peanuts and other types of nuts is prepared by adding 0.5 M sodium chloride solution to finely crushed powder of each material (raw peanut, roasted peanut, boiled peanut, walnut, almond, cashew nut, Was added to a glass beaker, and the mixture was extracted with boiling water for 15 minutes. After cooling at room temperature, the mixture was centrifuged at 3,220 x g for 15 minutes at 4 ° C. 1 filter paper, and the filtrate was stored frozen until the experiment.
  • SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis
  • the samples (3 ⁇ g per well) and protein markers (5 ⁇ l per well) (Prestained Broad Range Protein Maker, Bio-Rad, USA) were loaded on 5% stacking gel (pH 6.8) .
  • Electrophoresis was performed for the Mini-PROTEAN ® tetra electrophoresis cell ( Bio-Rad) and PowerPac TM Basic using (Bio-Rad) (1 hour and 30 minutes at 150V).
  • the gel was stained with a staining solution (EZblue TM gel staining reagent, Sigma) and discolored with water. Another gel was used for western blot analysis.
  • the proteins separated from the 12% separating gel were transferred to a nitrocellulose membrane (Bio-Rad) for 1 h at 100 V using a MiniTrans-Blot unit (Bio-Rad).
  • the membrane was blocked with blocking solution (3% skim milk) at 37 ° C for 1 hour and then washed 3 times with PBST.
  • the membrane was incubated at 37 ° C for 1 hour in a hybridoma cell culture (1: 4 in 3% skim milk) producing antibody, washed 4 times with PBST, and then incubated with a goat anti-mouse IgG alkali And reacted with a goat anti-mouse IgG alkaline phosphatase conjugate (Bio-Rad).
  • the membrane was illuminated by adding ECL (PerkinElmer, USA) and confirmed by an image analyzer.
  • ECL PerkinElmer, USA
  • an image analyzer In order to analyze whether the monoclonal antibody of the present invention binds to Ara h1, commercially available Ara h1 protein (20 ⁇ g / ml, INDOOR Biotechnologies, USA) was purchased and the binding force was analyzed.
  • Example 1 Preparation of a thermal stable-soluble protein in a peanut as an immunogen
  • the protein expression (lane 2) of the non-heat-treated peanut samples and the protein (lane 3) expression of the heat-treated peanut samples were significantly different from those of the non-heat treated peanuts by SDS-PAGE analysis And in all other samples, the main protein band appeared in the range of 15-100 kDa. As a result, it was confirmed that a thermostable water-soluble protein was present in peanuts extracted by the heat treatment method (FIG. 1).
  • the 3A1-12 antibody of the present invention was confirmed to be an antibody reactive with Ara h1. Since the molecular weight of Ara h1 currently known is 66 kDa, the target protein binding to the antibody of the present invention is identified as Ara h1.
  • Indirect ELISA was performed using the BP 3A1-12 and RP 4C12-10 antibodies of the present invention to determine the presence or absence of peanuts in the processed foods of Table 1 below.
  • the BP 3A1-12 and RP 4C12-10 antibodies of the present invention not only reacted to roasted peanut, boiled peanut and peanut butter, , Only the processed foods containing the peanut of Table 1 as a main component showed a specific reaction. In particular, it was confirmed that even when the concentration of the sample is diluted to 1/10, it can be detected.
  • the sensitivity of the BP 3A1-12 and RP 4C12-10 antibodies of the present invention was determined according to various peanut extract contents (0, 0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10, 100% Were measured by indirect ELISA.
  • the antibody of the present invention can detect the peanut protein at a concentration of 0.0001% (w / v) in the roasted peanut, boiled peanut and peanut butter extract (FIG. 6).
  • the monoclonal antibody produced in the hybridoma cells developed in the present invention reacted very specifically with the peanut protein, particularly the heat-stable protein, and it was found that the processed food containing various food additives, It is predicted that it will be useful for the future development of immune sensor or biosensor since it can quickly discriminate the presence of peanuts.

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Abstract

La présente invention concerne un marqueur permettant de déterminer la présence d'Arachis hypogaea 1 (Ara h1) dérivé de l'Arachis hypogaea dans des aliments contenant des Arachis hypogaea , ainsi que son utilisation. Un anticorps monoclonal de la présente invention, produit par une lignée cellulaire d'hybridome de numéro de dépôt KCTC 13295BP, et qui se lie spécifiquement à l'Arachis hypogaea derivée Ara h1, permet d'analyser efficacement de l' Arachis hypogaea mélangés dans des aliments.
PCT/KR2017/013320 2017-07-07 2017-11-22 Marqueur permettant la détermination de la présence d'arachis hypogaea dans des aliments et son utilisation WO2019009472A1 (fr)

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KR20210150168A (ko) * 2020-06-03 2021-12-10 경상국립대학교산학협력단 땅콩 유래 Ara h 1 및 Ara h 3에 특이적인 단일클론항체 및 이의 용도

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Cited By (2)

* Cited by examiner, † Cited by third party
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KR20210150168A (ko) * 2020-06-03 2021-12-10 경상국립대학교산학협력단 땅콩 유래 Ara h 1 및 Ara h 3에 특이적인 단일클론항체 및 이의 용도
KR102349848B1 (ko) 2020-06-03 2022-01-11 경상국립대학교산학협력단 땅콩 유래 Ara h 1 및 Ara h 3에 특이적인 단일클론항체 및 이의 용도

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