WO2018226051A2 - Composition de milieu sans sérum pour la culture de cellules comprenant un exosome dérivé d'une cellule souche humaine - Google Patents

Composition de milieu sans sérum pour la culture de cellules comprenant un exosome dérivé d'une cellule souche humaine Download PDF

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WO2018226051A2
WO2018226051A2 PCT/KR2018/006488 KR2018006488W WO2018226051A2 WO 2018226051 A2 WO2018226051 A2 WO 2018226051A2 KR 2018006488 W KR2018006488 W KR 2018006488W WO 2018226051 A2 WO2018226051 A2 WO 2018226051A2
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serum
stem cells
cell culture
cell
medium
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PCT/KR2018/006488
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Korean (ko)
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WO2018226051A3 (fr
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조용우
최지숙
이찬미
김재동
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주식회사 엑소스템텍
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Publication of WO2018226051A3 publication Critical patent/WO2018226051A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une nouvelle composition de milieu sans sérum pour la culture de cellules, un exosome qui induit l'adhésion et la prolifération cellulaires étant séparé d'une cellule souche humaine, et l'exosome étant contenu dans un milieu basique. Une composition de milieu sans sérum pour la culture de cellules selon la présente invention contient des gènes, des protéines et des facteurs de croissance associés à la croissance cellulaire, et peut ainsi favoriser l'adhésion et à la croissance de cellules même sans autres additifs tels que des activateurs cellulaires ou des facteurs de croissance, et peut induire et activer la prolifération cellulaire. La présente invention peut réduire les inconvénients du sérum à l'aide d'un milieu dans lequel un exosome est ajouté au lieu de sérum animal, ou en réduisant au minimum la quantité de sérum utilisée, et peut enfin être utilisée dans la culture de cellules souches pour une thérapie cellulaire dans une application clinique.
PCT/KR2018/006488 2017-06-07 2018-06-07 Composition de milieu sans sérum pour la culture de cellules comprenant un exosome dérivé d'une cellule souche humaine WO2018226051A2 (fr)

Applications Claiming Priority (2)

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KR10-2017-0070592 2017-06-07
KR20170070592 2017-06-07

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WO2018226051A2 true WO2018226051A2 (fr) 2018-12-13
WO2018226051A3 WO2018226051A3 (fr) 2019-03-21

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KR (1) KR102144593B1 (fr)
WO (1) WO2018226051A2 (fr)

Cited By (4)

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CN110938593A (zh) * 2020-01-02 2020-03-31 张伟 一种鳄鱼脂肪干细胞来源外泌体提取及冻干粉制备方法
CN113234663A (zh) * 2021-05-10 2021-08-10 博品(上海)生物医药科技有限公司 一种培养子宫内膜细胞的方法
CN114207116A (zh) * 2019-07-30 2022-03-18 韩国外泌体生技有限公司 新的外排体产生方法及其应用
CN114836375A (zh) * 2020-12-31 2022-08-02 国典(北京)医药科技有限公司 一种外泌体分泌诱导剂、诱导培养基及其应用

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WO2021091185A1 (fr) * 2019-11-05 2021-05-14 주식회사 한국줄기세포뱅크 Composition pour la culture cellulaire comprenant un facteur paracrine
KR102558281B1 (ko) * 2020-03-04 2023-07-25 주식회사 엠디헬스케어 락토바실러스 속 세균 유래 세포밖 소포를 유효성분으로 포함하는 줄기세포 기능 조절용 조성물
US20230151331A1 (en) * 2020-04-01 2023-05-18 Primoris Co., Ltd. Method for preparing culture medium containing high levels of high-potency exosomes secreted by cord blood stem cells, and use thereof
KR102253418B1 (ko) * 2020-10-08 2021-05-18 (주)지에프씨생명과학 피부 유산균 유래 엑소좀을 유효성분으로 포함하는 조성물 및 이의 제조방법
WO2022191341A1 (fr) * 2021-03-08 2022-09-15 주식회사 엑소스템텍 Composition pharmaceutique comprenant un exosome dérivé de cellules souches pour soulager ou traiter l'arthrite
KR20230150223A (ko) * 2022-04-20 2023-10-30 주식회사 아모그린텍 세포외 소포체 생산 시스템 및 세포외 소포체 생산방법

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KR20080042761A (ko) 2006-11-10 2008-05-15 문찬일 렙틴을 이용한 줄기세포 증식방법
KR101220239B1 (ko) 2008-04-01 2013-01-09 바이오스펙트럼 주식회사 식물성 펩톤을 포함하는 줄기세포 증식 촉진용 조성물
KR20110097064A (ko) * 2010-02-24 2011-08-31 (주) 아크스템 무혈청 줄기 세포 배양 조성물, 이를 함유한 조직 재생용 조성물 및 이를 이용한 조직 재생 방법
KR101026070B1 (ko) 2010-06-22 2011-03-31 주식회사 엘컴사이언스 피부줄기세포의 배양방법 및 상기 배양방법으로 얻어진 피부줄기세포를 포함하는 주름개선 또는 발모촉진용 조성물
KR101639988B1 (ko) * 2011-05-11 2016-07-15 (주)안트로젠 고농도의 세포성장인자를 포함하는 중간엽 줄기세포 배양액의 제조방법 및 이로부터 얻어진 조성물
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KR102153635B1 (ko) 2014-01-21 2020-09-08 주식회사 엘지생활건강 줄기세포 배양용 무혈청 배지 조성물 및 줄기세포의 배양방법
KR102153636B1 (ko) 2014-01-21 2020-09-08 주식회사 엘지생활건강 줄기세포 배양용 무혈청 배지 조성물 및 줄기세포의 배양방법
KR102153637B1 (ko) 2014-01-21 2020-09-08 주식회사 엘지생활건강 줄기세포 배양용 무혈청 배지 조성물 및 줄기세포의 배양방법
JP2017506511A (ja) * 2014-02-14 2017-03-09 ナショナル ユニバーシティー オブ アイルランド， ゴールウェイ 無血清培地
KR101629151B1 (ko) * 2014-11-07 2016-06-10 한양대학교 에리카산학협력단 줄기세포 유래 엑소좀을 포함하는 지방세포 분화유도 및 지방조직 재생용 조성물
KR101706642B1 (ko) * 2015-02-04 2017-02-17 주식회사 엑소스템텍 연골세포로 분화되고 있는 줄기세포로부터 추출된 엑소좀을 포함하는 연골세포 분화 유도 또는 연골조직 재생용 조성물
WO2017001649A1 (fr) * 2015-07-02 2017-01-05 Med Cell Europe Ag Sécrétomes et procédé de production de sécrétomes
KR101707622B1 (ko) 2016-11-18 2017-02-20 주식회사 디지레이 중간엽 줄기세포 증식용 무혈청 배지 조성물

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114207116A (zh) * 2019-07-30 2022-03-18 韩国外泌体生技有限公司 新的外排体产生方法及其应用
CN114207116B (zh) * 2019-07-30 2024-03-15 韩国外泌体生技有限公司 新的外排体产生方法及其应用
CN110938593A (zh) * 2020-01-02 2020-03-31 张伟 一种鳄鱼脂肪干细胞来源外泌体提取及冻干粉制备方法
CN114836375A (zh) * 2020-12-31 2022-08-02 国典(北京)医药科技有限公司 一种外泌体分泌诱导剂、诱导培养基及其应用
CN114836375B (zh) * 2020-12-31 2023-07-21 国典(北京)医药科技有限公司 一种外泌体分泌诱导剂、诱导培养基及其应用
CN113234663A (zh) * 2021-05-10 2021-08-10 博品(上海)生物医药科技有限公司 一种培养子宫内膜细胞的方法

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KR20180133812A (ko) 2018-12-17
KR102144593B1 (ko) 2020-08-13
WO2018226051A3 (fr) 2019-03-21

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