WO2018168581A1 - Répulsif contre les parasites, et procédé de fabrication de celui-ci - Google Patents

Répulsif contre les parasites, et procédé de fabrication de celui-ci Download PDF

Info

Publication number
WO2018168581A1
WO2018168581A1 PCT/JP2018/008629 JP2018008629W WO2018168581A1 WO 2018168581 A1 WO2018168581 A1 WO 2018168581A1 JP 2018008629 W JP2018008629 W JP 2018008629W WO 2018168581 A1 WO2018168581 A1 WO 2018168581A1
Authority
WO
WIPO (PCT)
Prior art keywords
pest repellent
fatty acid
producing
acid
metabolism
Prior art date
Application number
PCT/JP2018/008629
Other languages
English (en)
Japanese (ja)
Inventor
大野　勝也
久美子 高田
Original Assignee
イビデン株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イビデン株式会社 filed Critical イビデン株式会社
Priority to JP2019505910A priority Critical patent/JPWO2018168581A1/ja
Publication of WO2018168581A1 publication Critical patent/WO2018168581A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria

Definitions

  • the present invention relates to a pest repellent and a method for producing the pest repellent.
  • drugs such as pyrethrin, which is an active ingredient of pesticide chrysanthemums, and synthetic pyrethroids such as allethrin derived from pyrethrin derivatives have been used. While pyrethroids have low toxicity to mammals and birds, they act strongly on insect nerve cells to produce neurotoxins and kill insects. In recent years, neonicotinoid insecticides that kill insects by binding to the insect neurotransmitter acetylcholine receptor have been widely used as insecticides.
  • insecticidal method in which insects are directly killed using components that are generally toxic to such insects has a problem that even beneficial insects and natural enemy insects necessary for pollination are uniformly killed.
  • insecticides that have a long residual effect due to their ability to penetrate into plants such as neonicotinoids, may affect beneficial insects over a long period of time, and there is a concern about disruption of the ecosystem.
  • insecticide-resistant insects due to excessive application of agricultural chemicals is also a problem. From the viewpoint of environmental impact such as soil contamination, reduction of the amount of chemically synthesized pesticides is desired.
  • Patent Document 1 describes a microbial biosurfactant.
  • Patent Document 1 discloses a method for controlling pests using a fermentation broth filtrate containing rhamnolipid as a biosurfactant, which can be used as an insecticide for controlling house flies and nematodes. Are listed. However, in the method for controlling pests described in Patent Document 1, a drug must be applied directly to kill the pest, and therefore, it is necessary to apply the drug every time the pest is generated.
  • the conventional method of repelling pests is effective by gradually evaporating the drug, and has the advantage of widening the range of repelling pests, but the drug disappears due to volatilization. Therefore, there is a problem that the sustainability of the effect is short.
  • fatty acid oxides including lipid peroxides exhibit antibacterial action. It is also known that jasmonic acid and the like are biosynthesized in plants using fatty acids such as linolenic acid as raw materials. However, it is not known that fatty acid metabolites obtained by oxidizing fatty acids by microbial metabolism have an effect of inducing repellent effects in plants.
  • an object of the present invention is to provide a pest repellent that has low soil contamination and toxicity and can exhibit a long-term repellent effect in plants, and a method for producing the same.
  • the present invention relates to a pest repellent containing a fatty acid metabolite obtained by metabolizing fatty acids to proteobacteria.
  • the pest repellent is preferably a pest repellent which is a pest repellent secretion promoter in plants.
  • a pest repellent in which the fatty acid is a fatty acid having 4 to 30 carbon atoms is preferred.
  • a pest repellent in which the fatty acid is a fatty acid that is liquid at 20 ° C. is preferred.
  • a pest repellent is preferred in which the metabolism is metabolism in a dissolved oxygen concentration environment of 0.1 to 8 mg / l (liter).
  • a pest repellent in which the metabolism is metabolism in the presence of at least one mineral selected from Mg, P, Na and K is preferred.
  • a pest repellent is preferred in which the proteobacteria are pre-cultured proteobacteria.
  • Pest repellents are preferred wherein the pre-cultured proteobacteria are proteobacteria pre-cultured to 1 ⁇ 10 8 to 9 ⁇ 10 10 cells / ml (milliliter).
  • the pest repellent is preferably a pest repellent containing a biosurfactant.
  • a pest repellent in which the metabolism is metabolism under conditions of 20 to 30 ° C. is preferable.
  • the pest repellent is preferably a pest repellent that is used so as to come into contact with the foliage or roots of a plant.
  • the present invention also relates to a method for producing a pest repellent containing a fatty acid metabolite, which includes a fatty acid metabolism step of metabolizing fatty acid to proteobacteria.
  • a method for producing a pest repellent, wherein the pest repellent is a pest repellent secretion promoter in plants, is preferred.
  • a method for producing a pest repellent in which the fatty acid is a fatty acid having 4 to 30 carbon atoms is preferred.
  • a method for producing a pest repellent in which the fatty acid is a liquid fatty acid at 20 ° C. is preferred.
  • a method for producing a pest repellent in which the fatty acid metabolism step is performed in an environment of dissolved oxygen concentration of 0.1 to 8 mg / l is preferable.
  • a method for producing a pest repellent in which the fatty acid metabolism step is carried out in the presence of at least one mineral selected from Mg, P, Na and K is preferred.
  • a method for producing a pest repellent wherein the proteobacteria are pre-cultured proteobacteria is preferred.
  • a method for producing a pest repellent wherein the pre-cultured proteobacteria are pre-cultured to 1 ⁇ 10 8 to 9 ⁇ 10 10 cells / ml, is preferred.
  • a method for producing a pest repellent which is a method for producing a pest repellent containing a biosurfactant is preferred.
  • a method for producing a pest repellent in which the fatty acid metabolism step is carried out at 20 to 30 ° C. is preferable.
  • a method for producing a pest repellent which is a method for producing a pest repellent applied to plants is preferred.
  • the pest repellent of the present invention has low soil contamination and toxicity and is excellent in long-term repellent effect in plants. Moreover, according to the method for producing a pest repellent of the present invention, a pest repellent having low soil contamination and toxicity and excellent in long-term repellent effect in plants can be produced.
  • Pest repellent of the present invention is characterized by containing a fatty acid metabolite obtained by metabolizing fatty acids to proteobacteria.
  • resistance to the plant can be induced in addition to a direct pest repellent effect by a repellent substance that can be contained in the fatty acid metabolite.
  • a repellent substance that can be contained in the fatty acid metabolite.
  • the fatty acid metabolite contains, in addition to the repellent substance, a salicylic acid pathway related to systemic acquired resistance, a substance that activates the jasmonic acid pathway, or a precursor of this substance.
  • the plant itself can produce and / or secrete repellents, and the functions of the plant relating to the inherent insect repellent can be further developed.
  • the pest repellent of the present invention is a pest repellent secretion promoter containing a substance capable of promoting the secretion of a pest repellent from a plant.
  • the pest repellent of the present invention may further contain a repellent substance having a direct pest repellent effect in addition to the pest repellent secretion promoting substance.
  • the pest repellent of the present invention can exhibit a long-term repellent effect as compared with conventional pest repellents, and is also considered to be effective for pests that have developed drug resistance against conventional pesticides.
  • fatty acid metabolites are much less toxic than chemically synthesized pesticides.
  • Metabolism in the present invention means that fatty acids are decomposed by an enzyme or the like secreted by proteobacteria.
  • a method of culturing proteobacteria in a medium containing fatty acid can be mentioned.
  • Proteobacteria have a gene that produces lipoxygenase (LOX), an enzyme involved in fatty acid metabolism, and can produce fatty acid metabolites.
  • LOX lipoxygenase
  • the carbon number of the fatty acid used in the present invention is 4 to 30, and preferably 10 to 20.
  • the melting point / boiling point is low, and therefore, the volatility tends to increase at the temperature at the time of culturing and hardly remain in the medium.
  • the number of carbons exceeds 30, the melting point / boiling point becomes high, so that it becomes a solid at the temperature at the time of culturing and tends to be separated without being mixed with the medium.
  • the melting point may not depend only on the number of carbons depending on the number of hydrogen bonds.
  • the fatty acid used in the present invention is preferably a liquid at 20 to 30 ° C. and more preferably a liquid at 20 ° C. from the viewpoint of metabolic efficiency and suppression of solidification in the medium.
  • the fatty acid of the present invention can be a saturated fatty acid, an unsaturated fatty acid, or a mixture containing both.
  • a free fatty acid (monocarboxylic acid) is preferable because it is excellent in a decomposition rate.
  • Free fatty acids having 4 to 30 carbon atoms include butyric acid (butyric acid), valeric acid (valeric acid), caproic acid, enanthic acid (heptylic acid), caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, pentadecyl Acid, palmitic acid, palmitoleic acid, margaric acid, stearic acid, oleic acid, vaccenic acid, linoleic acid, ⁇ -linolenic acid, ⁇ -linolenic acid, eleostearic acid, arachidic acid, mead acid, arachidonic acid, behenic acid, Examples include lignoceric acid, nervonic acid, serotic acid, montanic acid, and melicic acid.
  • capric acid having 10 to 20 carbon atoms, lauric acid, myristic acid, pentadecylic acid, palmitic acid, palmitoleic acid, margaric acid, stearin Acid, oleic acid, vaccenic acid, linoleic acid, ⁇ -linolenic acid, ⁇ Linolenic acid, eleostearic acid, arachidic acid, mead acid, arachidonic acid are preferred, oleic acid having 18 carbon atoms, linoleic acid, alpha-linolenic acid, .gamma.-linolenic acid are more preferred.
  • the content of fatty acid is preferably 120 g / l or less, more preferably 100 g / l or less, and even more preferably 60 g / l or less. If it exceeds 120 g / l, emulsification with water in the medium becomes difficult, which may deteriorate metabolic efficiency and inhibit the growth of proteobacteria.
  • the lower limit of the fatty acid content is not particularly limited, but is preferably 1.0 g / l or more.
  • the medium containing fatty acid contains other mineral components.
  • a mineral component it is not specifically limited, The mineral component normally used for microorganism culture can be mentioned.
  • the component which has magnesium (Mg), phosphorus (P), sodium (Na), or potassium (K) is mentioned. These components can be used alone or in combination. Preferably two of these components may be used, more preferably three or more.
  • the content of the mineral component in the medium is not particularly limited, and can be the amount used in the conventional aerobic bacterium culture method, but since salt damage may occur during application to plants, preferably It can be used at 15 g / l or less, more preferably at 10 g / l or less.
  • proteobacteria used in the present invention is not particularly limited as long as the effects of the present invention are not impaired. From the viewpoint of fatty acid metabolism efficiency and growth efficiency, proteobacteria having a temperature suitable for growth (optimum temperature) of 10 to 40 ° C. are preferred, and proteobacteria having 20 to 30 ° C. are more preferred.
  • the proteobacteria are preferably pre-cultured proteobacteria because they are excellent in fatty acid metabolic efficiency, and the number of bacteria is preferably pre-cultured to 1 ⁇ 10 8 to 9 ⁇ 10 10 cells / ml. preferable.
  • the metabolism is preferably performed in a predetermined dissolved oxygen concentration environment.
  • the dissolved oxygen concentration in metabolism is preferably 0.1 mg / l or more.
  • a dissolved oxygen concentration is 8 mg / l or less, for example.
  • the dissolved oxygen concentration exceeds 8 mg / l, in parallel with the metabolic process by proteobacteria, decomposition of the fatty acid, which is a substrate, by oxygen in the medium proceeds, resulting in a decrease in metabolic efficiency and, consequently, an active ingredient. Metabolite production may be reduced.
  • the dissolved oxygen concentration is 0.1 to 8 mg / l, more preferably 0.1 to 5 mg / l, and still more preferably 0.1 to 4 mg / l.
  • the dissolved oxygen concentration is a value measured by a diaphragm galvanic electrode method or a diaphragm polarographic method on a PO electrode with a dissolved oxygen meter manufactured by HORIBA, Ltd.
  • the temperature in metabolism can be appropriately adjusted according to the proteobacteria used, but 20-30 ° C. is preferable from the viewpoint of fatty acid metabolic efficiency.
  • the insect repellent may contain a biosurfactant in addition to the fatty acid metabolite.
  • Fatty acid metabolites are easily dispersed in water, which is considered preferable from the viewpoint of handling properties of pest repellents.
  • the biosurfactant according to the present invention means a surfactant-like substance that is produced by a microorganism to take up a highly hydrophobic substance and secreted outside the cell.
  • the biosurfactant secreted by proteobacteria facilitates the dispersion of fatty acid metabolites in water so that spray treatment and irrigation of pest repellents containing fatty acid metabolites can be performed efficiently and easily. Become.
  • biosurfactant not only the biosurfactant produced by the proteobacteria of the present invention at the time of fatty acid degradation, but also biosurfactants produced by other microorganisms may be used, that is, the pest repellent of the present invention. Biosurfactants produced by other microorganisms may be further added. Compared to artificially synthesized surfactants, biosurfactants are less toxic to living organisms and have higher biodegradability, which is thought to provide a more environmentally friendly pest repellent. In addition, in order to promote fatty acid degradation by proteobacteria, biosurfactants produced by other microorganisms may be added during fatty acid degradation by proteobacteria. Fatty acid uptake by proteobacteria may be promoted.
  • the pest repellent of the present invention has an excellent effect as a pest repellent that is low in soil contamination and toxicity and can induce a long-term repellent effect in plants.
  • the pest repellent of the present invention activates a group of genes involved in the production of infection-specific proteins (pathogenesis-related (PR) protein) or a group of genes involved in the production of enzymes producing salicylic acid or jasmonic acid in the applied plant.
  • PR pathogenesis-related
  • enzymes producing salicylic acid or jasmonic acid in the applied plant To induce systemic acquired resistance. For example, it is estimated that the biosynthesis of secondary metabolites such as anti-pesticide protease inhibitors and insect repellents induced by jasmonic acid is increased.
  • the pest repellent of the present invention can express both upstream and downstream genes in both the salicylic acid pathway and the jasmonic acid pathway, resistance induction persists and a continuous repellent effect is obtained. It is advantageous.
  • the pest repellent of the present invention can induce the development of organs related to resistance to plant pests.
  • organ development is the increased formation of trichomes (trichomes).
  • Tricombs are known to develop by the activation of jasmonic acid signaling in response to injury, and the development of trichomes indicates that resistance has been induced in plants. Tricomb can release volatile pest repellent components from its tip. Therefore, by developing trichomes, volatile pest repellent components can be more efficiently diffused and the defense function of the plant can be enhanced. It is also known that some trichomes have a function of catching and catching insects depending on the species. Furthermore, since the increase in trichome density can also increase the insect repellent effect as a physical barrier against herbivorous insects, the pest repellent effect of the plant itself can be more activated.
  • the pest repellent of the present invention may further contain these precursors in addition to volatile aldehydes, alcohols and esters thereof, which are herbivore-induced volatile substances, in fatty acid metabolites.
  • Precursors can be converted to volatile aldehydes, alcohols and their ester forms by being absorbed by plants or decomposed in the natural environment. Therefore, the persistence of the repellent effect is thought to be due to the delay in the time from the application of the pest repellent to the plant until the precursor is converted to volatile aldehydes, alcohols and their esters. It is done.
  • the pest repellent of the present invention can be used to repel agricultural pests.
  • agricultural pests include, but are not limited to, pests such as aphids such as cotton aphids, spider mites, scarab beetles, stag beetles such as scallops, and bees such as chrysanthemum.
  • the pest repellent of the present invention exhibits a repellent effect even against the larvae of Spodoptera litura, which is known to have low susceptibility to insecticides, and is therefore effective against pests that have been conventionally difficult to control. It is excellent in that it can demonstrate.
  • the method for applying the pest repellent of the present invention to plants is not particularly limited.
  • it can be applied by spraying on the foliage or roots of the plant, dipping the foliage or roots of the plant, and / or soil irrigation on the soil in which the plant is growing.
  • the plant to be applied is not limited, and it can be used favorably for plants in general. Examples thereof include dicotyledonous plants such as eggplant, cucurbitaceae, and roseceae, and monocotyledonous plants such as gramineous.
  • the manufacturing method of the pest repellent containing the fatty acid metabolite of this invention is characterized by including the fatty acid metabolism process which metabolizes a fatty acid to proteobacteria.
  • the pest repellent obtained by the production method of the present invention is a pest repellent secretion promoter in plants. That is, a substance capable of promoting the secretion of a pest repellent substance from a plant can be produced by the fatty acid metabolism process of the present invention.
  • the fatty acid metabolism step of the present invention may be a step in which a repellent substance having a direct pest repellent effect is produced in addition to such a pest repellent secretion promoting substance.
  • the fatty acid metabolism step is a step in which fatty acids are decomposed by an enzyme or the like that is secreted or secreted by proteobacteria.
  • an enzyme or the like that is secreted or secreted by proteobacteria.
  • cultivating proteobacteria with the culture medium containing a fatty acid is mentioned.
  • the fatty acid metabolism step is preferably performed in a predetermined dissolved oxygen concentration environment.
  • the dissolved oxygen concentration in the fatty acid metabolism step is preferably 0.1 mg / l or more.
  • the dissolved oxygen concentration is less than 0.1 mg / l, the fatty acid resolution of proteobacteria tends to decrease and the metabolic efficiency of fatty acids tends to be extremely low.
  • it is preferable that a dissolved oxygen concentration is 8 mg / l or less, for example.
  • the dissolved oxygen concentration exceeds 8 mg / l, in parallel with the metabolic process by proteobacteria, decomposition of the fatty acid, which is a substrate, by oxygen in the medium proceeds, resulting in a decrease in metabolic efficiency and, consequently, an active ingredient.
  • the dissolved oxygen concentration is 0.1 to 8 mg / l, more preferably 0.1 to 5 mg / l, and still more preferably 0.1 to 4 mg / l.
  • the dissolved oxygen concentration is a value measured by a diaphragm galvanic electrode method or a diaphragm polarographic method on a PO electrode with a dissolved oxygen meter manufactured by HORIBA, Ltd.
  • the dissolved oxygen concentration can be adjusted by the culture vessel, the number of shakes, the amount of aeration, etc.
  • the culture conditions in the fatty acid metabolism step can be the same as the conventional conditions for culturing aerobic bacteria except that the dissolved oxygen concentration is within a predetermined range. Examples thereof include a method of culturing for 3 to 7 days by shaking with a flask and aeration culture with a spinner flask or a jar fermenter.
  • the number of days of culture is preferably the number of days during which the fatty acid is sufficiently emulsified and decomposed, but the number of days of culture varies depending on the agitation and the amount of bacteria.
  • the fatty acid decomposition state is determined by measuring the absorbance at a wavelength of 230 nm, thin layer chromatography (TLC), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry (GC / MS), liquid chromatography. It is preferable to confirm by graph mass spectrometry (LC / MS) or the like.
  • the temperature in the fatty acid metabolism step can be appropriately adjusted according to the proteobacteria used, and it is preferable to carry out the treatment at 20 to 30 ° C. from the viewpoint of fatty acid metabolic efficiency.
  • fatty acid and proteobacteria in the fatty acid metabolism step those described above in the description of the pest repellent of the present invention can be used.
  • the pre-culturing step for proteobacteria is not particularly limited, and a normal method for culturing aerobic bacteria can be used. It is preferable that only the cells are collected from the preculture solution by centrifugation or the like and used in the fatty acid metabolism step.
  • the pest repellent obtained by the production method of the present invention can contain a biosurfactant in addition to a fatty acid metabolite.
  • the biosurfactant according to the present invention means a surfactant-like substance that is produced by a microorganism to take up a highly hydrophobic substance and secreted outside the cell. Biosurfactants secreted by proteobacteria also facilitate the dispersion of fatty acid metabolites in water. It is thought that the handleability of the pest repellent of the present invention is improved.
  • the pest repellent of the present invention is obtained as a culture solution that is a mixture with a culture medium, a proteobacterium exocrine product containing a biosurfactant, microbial cells, and the like.
  • the culture solution may be used as it is as the pest repellent of the present invention, or the supernatant obtained by removing the cells from the culture solution by centrifugation or the like may be used as the pest repellent.
  • the pest repellent obtained by the production method of the present invention is applied to plants.
  • the culture solution can be used as it is. However, in the case of the stock solution, the treated portion of the plant at high temperatures may be condensed due to the evaporation of minerals and the effect of osmotic pressure. It may be desirable to do so.
  • the dilution factor is not particularly limited as long as the effect of the present invention is exhibited, but is preferably 10 to 1000 times diluted, more preferably 10 to 100 times diluted.
  • test pest repellent ⁇ pre-culture process 20 g of peptone (Difco protein enzyme hydrolyzate), 1.5 g of magnesium sulfate heptahydrate and 1.5 g of dipotassium hydrogen phosphate in 1 l (liter) of water in a glass Erlenmeyer flask, or 1 l (liter) ) 10 g of peptone (Difco protein enzyme hydrolyzate), 5 g of yeast extract and 10 g of sodium chloride are dissolved in water, autoclaved at 121 ° C. for 20 minutes, and cooled to room temperature. Inoculated. The mouth of the culture vessel was sealed with a silicon stopper.
  • the inoculated container was cultured for 24 hours under the conditions of 25 ° C. ⁇ 5 ° C. and 120 rpm using a bioshaker (BR-23UM manufactured by Taitec Corporation).
  • the number of bacteria in the culture was 5 ⁇ 10 8 cells / ml.
  • the cells were collected from the culture solution by centrifuging the culture solution under conditions of 15,000 ⁇ G and 20 ° C.
  • ⁇ Fatty acid metabolism process In 1 l (liter) of sterilized water in a glass Erlenmeyer flask, 12 g of linoleic acid (primary linoleic acid manufactured by Wako Pure Chemical Industries, Ltd.), 1.5 g of magnesium sulfate heptahydrate and / or dipotassium hydrogen phosphate 1 .5 g and the total amount of the cells obtained from the pre-culture step were added. This was cultured for 4 days under the conditions of 20 ° C., 120 rpm, and dissolved oxygen concentration of 4 mg / l using a bioshaker (BR-23UM manufactured by Taitec Corporation).
  • a bioshaker BR-23UM manufactured by Taitec Corporation
  • the degradation of linoleic acid involves measuring the concentration of oxidized lipid, which is one of linoleic acid intermediate products, in the culture solution using a spectrophotometer BioSpec-mini manufactured by Shimadzu Corporation at a wavelength of 230 nm. Confirmed by. After the culture, the following evaluation was performed using a culture solution containing the cells as a test pest repellent.
  • Examples 1-2 Cucumber true leaves having a length of about 10 cm at the longest part were cut into 2 cm squares and immersed in a test pest repellent (stock solution) and a 10-fold diluted solution thereof (Examples 1 and 2).
  • a petri dish (9 cm in diameter) was prepared by placing leaves immersed in a test pest repellent (test group) and leaves immersed in water (control group) one by one at intervals of 3 cm.
  • a filter paper (1 cm ⁇ 1 cm) was placed between the leaves in each petri dish, and 20 cotton aphids were placed on the filter paper.
  • Repellency (%) (1 ⁇ number of accumulated insects in test group / number of accumulated insects in control group) ⁇ 100 The results are shown in FIG.
  • Comparative examples 1 and 2 In place of the test pest repellent, a 0.1% solution of surfactin sodium (Comparative Example 1), which is a representative biosurfactant, and 0.4% of citronella, a citrus aroma component that is considered to have a repellent effect Treatment was observed in the same manner as in Examples 1 and 2 except that the solution (Comparative Example 2) was used. The results are shown in FIG.
  • the test group treated with the test pest repellent has a higher repellent rate compared to Surfactin sodium in Comparative Example 1 and Citronella in Comparative Example 2. Indicated. In particular, in Example 1 using the stock solution of the test pest repellent, the cotton aphid repellent rate reached 100%, and a strong cotton aphid repellent effect was confirmed. On the other hand, in the comparative example 1 using the biosurfactant, the repelling rate was only 68%, and in the comparative example 2 using the citronella, the repelling effect was 30%. Therefore, it can be seen that the pest repellent of the present invention has an excellent repellent effect.
  • Pest repellent effect in mini roses Example 3
  • a dilute solution of test pest repellent diluted 100 times with water is sprayed with leaves once a week (about 30 ml / strain) and soil Treated by irrigation (about 50 ml / strain).
  • the treatment was carried out for 6 to 7 months. After the treatment, naturally occurring pest parasites and food damage were examined. An untreated section was provided as a control. Pest infestation was evaluated by the average number of parasitic heads per strain or the number of parasitic leaves per strain. The eating damage was evaluated by the degree of insect damage.
  • the degree of insect damage is evaluated by five levels of 0 to 4 for the damage of each strain to be measured, the number of strains at each stage of damage is measured, and the numerical value (0 to 4) indicating the stage of damage to the number of strains is measured.
  • the total number multiplied by the number is divided by the total number of shares to be measured multiplied by 4 and multiplied by 100.
  • the stage of damage is defined as follows.
  • the number of leaves is 100 or more.
  • the stage 0 has no damaged leaves
  • the stage 1 has 3 damaged leaves
  • the stage 2 has 10 damaged leaves.
  • stage 3 is defined as a case where 30 or less leaves are damaged
  • stage 4 is a case where 30 or more leaves are damaged.
  • the pest repellent of the present invention has an excellent pest repellent effect.
  • the pest repellent of the present invention and the pest repellent produced by the production method of the present invention are pest repellents having low soil contamination and toxicity and excellent pest repellent effect.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

L'invention fournit un répulsif contre les parasites faible en termes de pollution des sols et de toxicité, mais présentant d'excellents effets d'induction de résistance et de répulsion des parasites, et fournit également un procédé de fabrication de ce répulsif contre les parasites. Plus précisément, l'invention concerne un répulsif contre les parasites qui contient un métabolite d'acide gras obtenu par métabolisme d'un acide gras en protéobactérie, et un procédé de fabrication de ce répulsif contre les parasites contenant un métabolite d'acide gras qui inclut une étape de métabolisme d'acide gras au cours de laquelle un acide gras est métabolisé en protéobactérie.
PCT/JP2018/008629 2017-03-14 2018-03-06 Répulsif contre les parasites, et procédé de fabrication de celui-ci WO2018168581A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019505910A JPWO2018168581A1 (ja) 2017-03-14 2018-03-06 害虫忌避剤および害虫忌避剤の製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017-048720 2017-03-14
JP2017048720 2017-03-14

Publications (1)

Publication Number Publication Date
WO2018168581A1 true WO2018168581A1 (fr) 2018-09-20

Family

ID=63522970

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2018/008629 WO2018168581A1 (fr) 2017-03-14 2018-03-06 Répulsif contre les parasites, et procédé de fabrication de celui-ci

Country Status (2)

Country Link
JP (1) JPWO2018168581A1 (fr)
WO (1) WO2018168581A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06199616A (ja) * 1992-12-29 1994-07-19 Tsurumi Soda Co Ltd 害虫忌避剤
JP2002504141A (ja) * 1997-06-16 2002-02-05 アグラクエスト，インコーポレイテッド 平胸類オイルまたはその活性画分を含有する組成物およびそれら組成物の昆虫忌避剤としての使用方法
WO2015029872A1 (fr) * 2013-08-26 2015-03-05 学校法人上智学院 Répulsif servant à repousser les nématodes à galles, procédé pour sa fabrication et procédé de répulsion utilisant le répulsif
WO2018047918A1 (fr) * 2016-09-08 2018-03-15 イビデン株式会社 Activateur pour végétaux, et procédé de fabrication de celui-ci

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06199616A (ja) * 1992-12-29 1994-07-19 Tsurumi Soda Co Ltd 害虫忌避剤
JP2002504141A (ja) * 1997-06-16 2002-02-05 アグラクエスト，インコーポレイテッド 平胸類オイルまたはその活性画分を含有する組成物およびそれら組成物の昆虫忌避剤としての使用方法
WO2015029872A1 (fr) * 2013-08-26 2015-03-05 学校法人上智学院 Répulsif servant à repousser les nématodes à galles, procédé pour sa fabrication et procédé de répulsion utilisant le répulsif
WO2018047918A1 (fr) * 2016-09-08 2018-03-15 イビデン株式会社 Activateur pour végétaux, et procédé de fabrication de celui-ci

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OHNO, KATSUYA: "Approach of Plant Responses to Plant Disease Control by Bacterial Metabolites of Fatty Acid (BMFA) (1", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2017 (2017-03-05), pages 07 *

Also Published As

Publication number Publication date
JPWO2018168581A1 (ja) 2020-05-14

Similar Documents

Publication Publication Date Title
Pradhanang et al. Effects of plant essential oils on Ralstonia solanacearum population density and bacterial wilt incidence in tomato
US20220295713A1 (en) Plant activator and a method of manufacturing the same
JP5699153B2 (ja) 植物保護用および工業非農作物分野用のアジュバント／添加剤として農薬と併用するソホロリピドおよびその誘導体の使用
US10674731B2 (en) Package for plant antimicrobial treatment
JPH06507399A (ja) 確立された植物感染を抑制するための脂肪酸基盤の組成物
US8329617B2 (en) Compositions and methods for synergistic manipulation of plant and insect defenses
GB2541935A (en) Insecticide/miticide composition
WO2009091564A1 (fr) Utilisation d'agents tensio-actifs d'origine biologique pour atténuer les dégâts subis par les plantes à cause des nuisibles
MX2011004092A (es) Composiciones de cinamaldehiido-alacina y su metodo de uso.
JP2005029489A (ja) 殺虫殺卵組成物
WO2014193162A1 (fr) Procédé de préparation d'un biopesticide au moyen d'une souche de paecilomyces sp.
WO2018168581A1 (fr) Répulsif contre les parasites, et procédé de fabrication de celui-ci
JP7354382B2 (ja) トマト果実の成長促進剤およびトマト果実中の機能性成分含有量向上剤、ならびに、トマト果実の成長促進剤およびトマト果実中の機能性成分含有量向上剤の製造方法
WO2018168582A1 (fr) Pesticide, et procédé de fabrication de celui-ci
JPH0748218A (ja) 植物の病害を防除する方法
JP2007082513A (ja) 抗菌性物質を含有する発酵液及びその製造方法並びに抗菌性物質の製造システム
WO2020180490A1 (fr) Formulation microbienne d'intestins d'abeilles destinée à être utilisée en tant que probiotique pour améliorer la santé et la résistance aux agents pathogènes des abeilles
US20180014537A1 (en) Novel compositions and methods for controlling soil borne pathogens of agricultural crops
EP3166400A1 (fr) Mélange et procédé de lutte biologique destinés à améliorer la qualité des ufs en coquille
RU2333644C1 (ru) Способ борьбы с колорадским жуком и средство для его осуществления
CN114831140A (zh) 西藏亚菊提取物在制备除草剂和/或杀虫剂中的应用
CN113826635A (zh) 一种增效型杀菌剂及其应用
JP2018188369A (ja) ネコブセンチュウ防除剤
Mohan et al. Natural enemy complex associated with coconut eriophyid mite, Aceria guerreronis Keifer
JPH1045520A (ja) 微生物含有雑草防除組成物及び除草方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18767569

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2019505910

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18767569

Country of ref document: EP

Kind code of ref document: A1