WO2018155952A3 - 인간 뇌 조직 유래 신경줄기세포의 고효율 분리 방법 - Google Patents

인간 뇌 조직 유래 신경줄기세포의 고효율 분리 방법 Download PDF

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Publication number
WO2018155952A3
WO2018155952A3 PCT/KR2018/002253 KR2018002253W WO2018155952A3 WO 2018155952 A3 WO2018155952 A3 WO 2018155952A3 KR 2018002253 W KR2018002253 W KR 2018002253W WO 2018155952 A3 WO2018155952 A3 WO 2018155952A3
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Prior art keywords
neural stem
high efficiency
stem cell
brain tissue
human brain
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PCT/KR2018/002253
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English (en)
French (fr)
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WO2018155952A9 (ko
WO2018155952A2 (ko
Inventor
주경민
남현
남도현
홍승철
연제영
Original Assignee
주식회사 메디노
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Application filed by 주식회사 메디노 filed Critical 주식회사 메디노
Priority to CN201880013767.4A priority Critical patent/CN110536962A/zh
Priority to US16/487,458 priority patent/US20190374681A1/en
Publication of WO2018155952A2 publication Critical patent/WO2018155952A2/ko
Publication of WO2018155952A3 publication Critical patent/WO2018155952A3/ko
Publication of WO2018155952A9 publication Critical patent/WO2018155952A9/ko

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/383—Nerve cells, e.g. dendritic cells, Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

본 발명은 신경줄기세포를 신속하게 대량으로 증식시키고 고효율로 분리할 수 있는 신경줄기세포 배양 및 분리 방법 및 이에 의해 배양되고 분리된 뇌졸중 환자로부터 유래된 이식용 인간 성체 신경줄기세포에 대한 것이다.
PCT/KR2018/002253 2017-02-24 2018-02-23 인간 뇌 조직 유래 신경줄기세포의 고효율 분리 방법 WO2018155952A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201880013767.4A CN110536962A (zh) 2017-02-24 2018-02-23 高效分离人脑组织来源的神经干细胞的方法
US16/487,458 US20190374681A1 (en) 2017-02-24 2018-02-23 Method for isolating human brain tissue-derived neural stem cell at high efficiency

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020170024657A KR101910269B1 (ko) 2017-02-24 2017-02-24 인간 뇌 조직 유래 신경줄기세포의 고효율 분리 방법
KR10-2017-0024657 2017-02-24

Publications (3)

Publication Number Publication Date
WO2018155952A2 WO2018155952A2 (ko) 2018-08-30
WO2018155952A3 true WO2018155952A3 (ko) 2019-02-07
WO2018155952A9 WO2018155952A9 (ko) 2019-03-21

Family

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PCT/KR2018/002253 WO2018155952A2 (ko) 2017-02-24 2018-02-23 인간 뇌 조직 유래 신경줄기세포의 고효율 분리 방법

Country Status (4)

Country Link
US (1) US20190374681A1 (ko)
KR (1) KR101910269B1 (ko)
CN (1) CN110536962A (ko)
WO (1) WO2018155952A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101994640B1 (ko) * 2018-03-16 2019-07-02 (주)메디노 신경줄기세포의 고효율 분리배양 방법
CN114181926B (zh) * 2021-12-10 2023-11-24 上海欧易生物医学科技有限公司 一种适用于酶解小鼠脑组织的酶解液、细胞分离的方法及其应用

Citations (3)

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KR20110090810A (ko) * 2010-02-03 2011-08-10 사회복지법인 삼성생명공익재단 노치 신호 활성 유전자를 이용한 줄기세포의 증식 방법
KR20130109999A (ko) * 2012-03-28 2013-10-08 고려대학교 산학협력단 신경줄기세포 배양액 또는 신경줄기세포 추출물을 유효성분으로 함유하는 미백용 화장료 조성물 및 이의 제조방법
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ES2606544T3 (es) * 2010-02-03 2017-03-24 Samsung Life Public Welfare Foundation Método para provocar la proliferación de células madre mediante la activación de la señalización de Notch
KR20130119683A (ko) * 2012-04-24 2013-11-01 사회복지법인 삼성생명공익재단 줄기세포 배양 배지 및 이를 이용한 줄기세포의 배양방법

Patent Citations (3)

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KR20110090810A (ko) * 2010-02-03 2011-08-10 사회복지법인 삼성생명공익재단 노치 신호 활성 유전자를 이용한 줄기세포의 증식 방법
KR20130109999A (ko) * 2012-03-28 2013-10-08 고려대학교 산학협력단 신경줄기세포 배양액 또는 신경줄기세포 추출물을 유효성분으로 함유하는 미백용 화장료 조성물 및 이의 제조방법
KR20130143532A (ko) * 2012-06-21 2013-12-31 사회복지법인 삼성생명공익재단 환자 맞춤형 교모세포종 동물 모델의 제조방법 및 이의 용도

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Also Published As

Publication number Publication date
US20190374681A1 (en) 2019-12-12
WO2018155952A9 (ko) 2019-03-21
CN110536962A (zh) 2019-12-03
KR20180105278A (ko) 2018-09-28
KR101910269B1 (ko) 2018-10-19
WO2018155952A2 (ko) 2018-08-30

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