WO2018131930A1 - Composition pour la prévention ou le traitement de la coccidiose, son procédé de production et utilisation associée - Google Patents

Composition pour la prévention ou le traitement de la coccidiose, son procédé de production et utilisation associée Download PDF

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Publication number
WO2018131930A1
WO2018131930A1 PCT/KR2018/000613 KR2018000613W WO2018131930A1 WO 2018131930 A1 WO2018131930 A1 WO 2018131930A1 KR 2018000613 W KR2018000613 W KR 2018000613W WO 2018131930 A1 WO2018131930 A1 WO 2018131930A1
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composition
coccidiosis
present
preventing
feed
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PCT/KR2018/000613
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English (en)
Korean (ko)
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차장옥
차영서
차가송
구용주
김동중
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차장옥
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/12—Ketones
    • A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
    • A61K9/2806—Coating materials
    • A61K9/282—Organic compounds, e.g. fats
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4891—Coated capsules; Multilayered drug free capsule shells

Definitions

  • the present invention comprises anethol (Anethol) or a pharmaceutically acceptable salt thereof, and a porous adsorbent as an active ingredient, feed composition for the prevention or treatment of Coccidiosis (Coccidiosis); Pharmaceutical compositions; Emereria tenella insecticidal composition; And methods for preventing or treating coccidiosis.
  • the present invention feed composition for the prevention or treatment of coccidiosis comprising an acid or an acid extract as an active ingredient; Pharmaceutical compositions; And methods for preventing or treating coccidiosis.
  • Coccidiosis is a type of parasitic disease characterized by diarrhea, enteritis, and bloody stools found in almost all animals, and the Eimeria protozoa belonging to the spores.
  • the Amerian protozoa is an evolved protozoa among protozoa and has sexual and asexual genitalia and exhibits pathogenicity to asexual genitals. It is rare to be caused by one species of protozoa. In general, mixed infections are observed outdoors, and if only one species is immunized, it does not defend against other species.
  • Coccidiosis inhibits development in chickens and is most commonly found in chicks between 21 and 40 days of age.
  • the mortality rate varies depending on the type of protozoa, but the infection rate and mortality rate is very high between 28 and 40 days of age, and economic loss is a big problem when economic animals suffer from coccidiosis.
  • the present inventors have diligently sought to find an economical Aymeric insect repellent among edible natural products.
  • the natural compound sancho has the effect of the insecticidal Tenerella insecticidal effect, and in the case of anetol derived therefrom, Due to the unstable nature of the change in exposure to air, the process of adsorption and coating is important, through which it was confirmed that can effectively enhance the effect of anethol, the present invention was completed.
  • Patent Document 1 Republic of Korea Patent No. 10-0617415
  • the present inventors have diligently sought to find an economical Aymeric insect repellent among edible natural products.
  • the natural compound sancho has the effect of the insecticidal Tenerella insecticidal effect, and in the case of anetol derived therefrom, Due to the unstable nature of the change in exposure to air, the process of adsorption and coating is important, through which it was confirmed that can effectively enhance the effect of anethol, the present invention was completed.
  • An object of the present invention is a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof; And a porous adsorbent; feed composition for preventing or treating coccidiosis comprising an active ingredient; Pharmaceutical compositions; Aymeric Tenella insecticidal composition; And it provides a method for preventing or treating coccidiosis.
  • Another object of the present invention is a feed composition for the prevention or treatment of coccidiosis comprising an acid or sancho extract as an active ingredient; Pharmaceutical compositions; And it provides a method for preventing or treating coccidiosis.
  • a composition comprising anetitol and adsorbent of the present invention as an active ingredient; Sancho; And it has a prophylactic or therapeutic effect of Sancho extract coccidiosis, and thus can be widely used in feed compositions, pharmaceutical compositions, insecticidal compositions, and methods for treating coccidiosis.
  • 1 is a graph showing the number of eggs from feces of chickens infected with Aimerian tenella after the administration of Sancho dry matter in chronological order (day, day).
  • Figure 2 is a graph showing the number of eggs from the feces of chicken worms infected with Aymerhenella after administration of anetol alone in chronological order (day, day).
  • Figure 3 is a graph showing the number of eggs in the stool of the chicks infected with Aymerhenella after the administration of anetol / charcoal in chronological order (day, day).
  • Figure 4 is a graph showing the number of eggs released on the 7th day of infection with Aymeric Tenella according to the anetol concentration.
  • the present invention to solve the above problems and to achieve the object of the present invention, a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof; And a porous adsorbent; provides a feed composition for preventing or treating coccidiosis.
  • the compound of Formula 1 is a kind of ether called anethol, and is present in scent oil, anise oil, fennel oil and the like. At room temperature, it exists in the form of white crystals and has a distinctive fragrance and strong sweetness.
  • Anetol which is found in nature, corresponds to a trans form, and may be used as a fragrance for confectioneries and beverages, or may be used for fragrances, nutrients, and expectorants.
  • Anetol when left at room temperature, changes to anisealdehyde, aniseic acid, etc. by air and light. In this case, it loses its crystallinity and becomes yellowish, turning it into a hot and unpleasant taste.
  • anetol is used in various fields and can be obtained in nature, but it is noted as a useful material, but due to its unique unstable properties, changes in form and properties easily occur, and there is a problem that it is difficult to use it for purposes of controlling.
  • the present invention is to recognize and compensate for the instability of the anetol as described above.
  • the anetol when the anetol is adsorbed on the porous adsorbent and used in the state where the anetol is bound to the pores, it is possible to prevent the change of the properties of the anetol at room temperature.
  • Anetol was stably delivered to the cecum and killed Ameria Tenellae parasitic in the cecum, thereby confirming that coccidiosis could be effectively treated.
  • Coccidiosis of the present invention may be caused by Emereria tenella .
  • Coccidiosis of the present invention may be coccidiosis of chicken (or chick).
  • the coccidiosis is a disease in which Coccidia genus of Eimeria is parasitic in the cytoplasm of the submucosal tissue in the digestive tract epithelium and destroys the epithelium and causes enteritis, and in particular, decreases the weight gain rate in broiler farms to stool, diarrhea and bloody stool. It is a protozoan disease that causes economic damage by prolonging the shipping date. Coccidiosis can be expressed not only in broilers, but also in birds and mammals. Specifically, it can infect cattle, rabbits, goats, dogs, and cats, and can cause damage to experimental animals such as mice and rats. Mainly wear.
  • Coccidiosis in chickens can be infected by nine protozoa, the main one being Eimeria tenella), child Almeria acetate or unfavorable (Eimeria acevulina), four children Almeria cart Riggs (Eimeria vecatrix ), Emeria mexima ( Eimeria maxima ), Emereria brunetti ( Eimeria brunetti ) may be five, and specifically may be by Emeria Tenella .
  • the coccidiosis includes acute, subacute, chronic, and the like, and the coccidiosis of the present invention may include acute coccidiosis, subacute coccidiosis, chronic coccidiosis, and the like, and specifically, may be acute coccidiosis.
  • blood infections are confirmed within 48 hours after the infection with Aymeric Tenella, and in addition to loss of energy, anorexia, anemia, and eventually death.
  • Coccidial cysts are infectious when they mature into Sporulated Oocysts at high humidity and temperature.They spread easily by excretion of the eggs into the feces after a certain life cycle in the chicken's body. It is done and the life of strig is repeated.
  • the feed composition of the present invention comprises a porous adsorbent.
  • the compound represented by Chemical Formula 1 may be included in the composition in the form adsorbed on the porous adsorbent, and when included in the feed, it can be effectively delivered to the cecum parasitic parasitic coccidium within 21 hours after ingestion. In other words, it is possible to obtain an excellent therapeutic effect of coccidiosis by delivering anet to a stable state with a porous adsorbent.
  • the porous adsorbent refers to a solid material having a large internal surface area and adsorbing harmful components present in air, water, and soil.
  • the porous adsorbent is charcoal, Molecular sieves 13X 10 ⁇ , Molecular sieves 5 ⁇ , bentonite, molasses, kaolin Or zeolite, and specifically, may be charcoal, but is not limited thereto.
  • Chacoal is a solid product that is produced when the air supply is shut off and the wood is heated or with less air. By using charcoal, the compound represented by Chemical Formula 1 can be effectively delivered to the cecum.
  • the effect of Imerian tenella insecticidal appears to be excellent. It was confirmed.
  • the compound represented by the formula (1) effectively adsorbs to the parasitic cecum that is given by Ameria tenella, and as a result, any adsorbent capable of exhibiting the therapeutic effect of coccidiosis can be used without limitation to the type of adsorbent.
  • the porous adsorbent may use a porous material having a pore size of 3 kPa to 13 kPa, and more specifically, the pore size may be 5 kPa or 10 kPa. Under such pore size conditions, porous adsorbed compounds can be effectively delivered to the cecum where parasitic Ameria tenellae are parasitic.
  • 20 g of charcoal is used for 20 ml of the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof, or 50 g of charcoal is used for 10 ml of the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof. It was confirmed that the composition of the prepared using showed excellent effect of the insecticidal tenella insecticidal.
  • the volume ratio of the compound represented by Formula 1 and the mass ratio of the porous adsorbent are appropriately selected by those skilled in the art for the effective therapeutic effect of coccidiosis in consideration of the type of the porous adsorbent, the dosage of the composition, the amount of the compound represented by Formula 1, and the like. Can be used.
  • the feed composition for preventing or treating coccidiosis of the present invention may further include vegetable oil.
  • the vegetable oil may be for feed or edible oil, specifically, soybean oil, corn oil, rapeseed oil, olive oil, sesame oil, perilla oil, safflower oil, sunflower oil, palm oil, or palm oil, but are not limited thereto.
  • the excellent Aymeric Tenella insecticidal effect by the composition coated with 50ml soybean oil for 20g charcoal.
  • the mass ratio of the porous adsorbent to the volume of the vegetable oil is appropriately selected by those skilled in the art for the effective therapeutic effect of coccidiosis in consideration of the type of porous adsorbent, the type of vegetable oil, the amount of the compound represented by the formula (1), the dosage of the composition, and the like. Can be.
  • the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof may be included in the feed composition of the present invention at a concentration of 5 to 2000 ppm and 10 to 2000 ppm, but is not limited thereto.
  • the concentration of the compound represented by the formula (1) in the feed composition is 100, 500, and 1000ppm, respectively, in order to confirm the insecticidal effect on the Aimeric tenella according to the concentration of the compound represented by the formula (1)
  • “Pharmaceutically acceptable salt” of the present invention means a compound that does not impair the biological activity and physical properties of the compound represented by the formula (1) to be administered.
  • Non-limiting examples of such pharmaceutically acceptable salts include acids that form non-toxic acid addition salts containing pharmaceutically acceptable anions such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrobromic acid, hydroiodic acid, and the like.
  • Organic carbon acids such as inorganic acid, tartaric acid, formic acid, citric acid, acetic acid, trichloroacetic acid, trichloroacetic acid, gluconic acid, benzoic acid, lactic acid, fumaric acid, maleic acid, salicylic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, acid addition salts formed by sulfonic acids and the like such as p-toluenesulfonic acid and the like.
  • carboxylic acid salts include metal salts or alkaline earth metal salts formed by lithium, sodium, potassium, calcium, magnesium, amino acid salts such as lysine, arginine, guanidine, dicyclohexylamine, N-methyl-D-glu Organic salts such as carmine, tris (hydroxymethyl) methylamine, diethanol, amine, choline, triethylamine, and the like, and the like, but are not limited thereto.
  • prevention of the present invention means any action that suppresses or delays the symptoms caused by the coccidiosis of the present invention.
  • treatment of the present invention means all the actions to improve or cure the symptoms caused by the coccidiosis of the present invention.
  • feed of the present invention may refer to any natural or artificial diet, one meal, or the like or any ingredient of the one meal for the animal to eat, ingest and digest.
  • the feed composition of the present invention may further include a thick feed, and / or special feed.
  • the rich feed includes seed fruits containing grains such as wheat, oats and corn, and by-products obtained by refining grains, bran, beans, fluids, sesame seeds, linseed, coco, etc., including rice bran, bran and barley bran.
  • Fish soluble which is a concentrate of fresh liquids obtained from fish residues, fish residues, and fish residues such as by-products such as seaweed, sweet potatoes, and potatoes, which are the remaining components of starch residue. soluble), meat meal, blood meal, milk powder, skim milk powder, milk from cheese, milky cheese, and dried whey, which is the balance of manufacturing casein from dried milk, animal feeds such as dried whey, yeast, chlorella, seaweed, etc. have.
  • Feed composition of the present invention may mean a dietary supplement, or a feed additive.
  • the dietary supplement may be, for example, a formulation containing a composition for providing a therapeutic agent or a digestive agent to an animal.
  • the dietary supplement may be a composition that is ingested in addition to a conventional animal feed, although it is not a calorie intake source of a living body, that is, an energy source.
  • the feed additive means a substance added to the feed for various effects such as supplementation of nutrients and prevention of weight loss, enhancement of digestive availability of fiber in the feed, improvement of oil quality, prevention of reproductive disorders and improvement in conception rate, and prevention of high temperature stress in summer. In the present invention, it means a substance added for the purpose of preventing or treating coccidiosis by Aimeria tenella.
  • composition of the present invention may further comprise a glue, gelatin, agar, casein, sodium alginate, xanthan gum, queen's seed gum, hydroxy cellulose, sodium carboxymethyl cellulose, or a combination thereof.
  • composition may further include molasses.
  • the molasses is a generic term for a liquid containing sucrose produced as a by-product when preparing sugar, and the molasses may be used for food or feed.
  • Another embodiment of the present invention is a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof; And a porous adsorbent; provides a pharmaceutical composition for preventing or treating coccidiosis.
  • the compound represented by the formula (1) pharmaceutically acceptable salts, porous adsorbents, Aimeric tenella, coccidiosis, prevention, and treatment are as described above.
  • the term "pharmaceutical composition” means prepared for the purpose of preventing or treating a disease, and may be administered in various oral and parenteral dosage forms in actual clinical administration. , Diluents or excipients such as extenders, binders, wetting agents, disintegrants, surfactants and the like.
  • the pharmaceutical composition of the present invention may also be used as a single agent, and may be prepared and used as a complex preparation, further including a pharmaceutical composition known to have a certified preventive or therapeutic effect of coccidiosis.
  • Pharmaceutically acceptable carriers, excipients, or diluents may be added to form a pharmaceutical unit dosage form.
  • the term "pharmaceutically acceptable” means that it does not significantly irritate the organism and does not inhibit the biological activity and properties of the administered active substance.
  • the pharmaceutical composition comprising a pharmaceutically acceptable carrier in the present invention is a tablet, pills, powders, granules, capsules, suspensions, solvents, emulsions, syrups, sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freezing It may have any one formulation selected from the group consisting of a desiccant and suppositories.
  • the pharmaceutical composition may be in various oral or parenteral formulations. When formulated, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used.
  • Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose (at least one compound). lactose), gelatin and the like can be mixed. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like can also be used.
  • Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups, and various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin, may be included. have.
  • Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories.
  • non-aqueous solvent and the suspension solvent propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used.
  • base of the suppository witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.
  • Another embodiment of the present invention is a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof; And a porous adsorbent; provides a composition for insecticidal insects of Emereria tenella ( Eimeria tenella ) comprising as an active ingredient.
  • the compound represented by the formula (1), the pharmaceutically acceptable salt, the porous adsorbent, and the Emeria tenella are as described above.
  • composition represented by the formula (1) of the present invention or a pharmaceutically acceptable salt thereof, and a porous adsorbent composition has an insecticidal effect on Ameria tenella, and may be used to prevent coccidiosis by Ameria tenella. It can be usefully used for therapeutic purposes.
  • Another aspect of the invention provides a method for preventing or treating coccidiosis comprising administering the composition to a chicken.
  • the feed composition, the pharmaceutical composition, the insecticidal composition, Ameria tenella, coccidiosis, prevention, and treatment are as described above.
  • composition of the present invention may be administered in a pharmaceutically effective amount.
  • the pharmaceutically effective amount refers to an amount sufficient to treat the disease at a reasonable benefit / risk ratio applicable to medical treatment and not to cause side effects
  • the effective dose level refers to an individual's health condition, type of disease, severity, The activity of the drug, the sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of excretion, the duration of treatment, the factors including the drug used in combination or co-administration, and other factors well known in the medical art.
  • administration means introducing a predetermined substance into an individual in an appropriate manner and the route of administration of the composition can be administered via any general route as long as it can reach the target tissue.
  • Intraperitoneal administration intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, nasal administration, pulmonary administration, rectal administration, but is not limited thereto.
  • individual means any animal that develops or can develop coccidiosis, and may specifically be poultry including chicken.
  • compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. And single or multiple administrations. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect in a minimum amount without side effects, and can be easily determined by those skilled in the art.
  • Another aspect of the present invention provides a feed composition for preventing or treating coccidiosis, which comprises an acid or an extract of the extract as an active ingredient.
  • Coccidiosis prophylaxis
  • Zanthoxylum of the present invention schinifolium is a deciduous shrub belonging to rhyme and bark, and it is used as a raw material by picking leaves, peeled and dried, and then dried or concentrated. Fruits are used as raw materials for making oil, edible or medicinal.
  • the branched branches have a pair of thorns and the leaves are shifted into a pinnate double leaf consisting of 5-9 pairs of small leaves, each of which is egg-shaped or lanceolate, and grows well in the sun at the foot of the mountain.
  • the acid is known to be effective in indigestion, planting, gastric drainage, gastric dilatation, vomiting, dysentery, diarrhea, nervous breakdown and cough, coccidiosis, particularly coccidiosis caused by Aymeria tenella
  • the therapeutic effect is not known at all, which was first identified by the inventors.
  • Sancho of the present invention can be obtained by using commercially available or directly grown and obtained route is not a problem, all parts such as fruit, juice, seeds, flowers, leaves, rhizomes, stems, roots are available, and As the fruit portion can be used, but is not limited thereto.
  • the term "sancho" of the present invention may be used to include all natural products themselves, dried products thereof, crushed products thereof, and the like, as long as they can exhibit the effects of preventing or treating coccidiosis, but are not limited to the type, size, moisture content, shape, etc. Can be used without.
  • extract refers to a formulation obtained by squeezing a crude drug with an appropriate leachate and evaporating the leachate to concentrate the extract, a dilution or concentrate of the extract, a dried product obtained by drying the extract, These may be crude or purified products.
  • the sancho extract can be prepared using common extraction methods, separation and purification methods known in the art. Specifically, as the extraction method, hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used.
  • the extract may be prepared by extracting with an extracting solvent or by fractionating the fractional solvent to an extract prepared by extracting with an extracting solvent.
  • the extraction solvent may be at least one solvent selected from the group consisting of water, C1 to C4 lower alcohols, 1,3-butylene glycol, and ethyl acetate, and specifically, the lower alcohol may be ethanol, methanol, propanol, Or butanol.
  • the extraction solvent may be extracted by adding 1 to 10 times the amount of dried acidic acid, specifically, may be extracted by adding 2 to 3 times.
  • Extraction temperature may be 30 °C to 100 °C, specifically may be 50 °C to 90 °C.
  • the extraction time may be 10 hours to 48 hours, specifically 15 hours to 30 hours.
  • the extraction frequency may be 1 to 5 times, specifically, may be extracted repeatedly 3 to 4 times.
  • the preparation step of the extract may further include a step of condensing the extract under reduced pressure, the decompression concentration may use a vacuum pressure reducer or vacuum rotary evaporator.
  • the manufacturing step may further include the step of drying the extract subjected to the reduced pressure concentration process, the drying may be reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto. .
  • Another aspect of the present invention provides a pharmaceutical composition for preventing or treating coccidiosis, which comprises an acid or an extract of an acid extract as an active ingredient.
  • Another aspect of the invention provides a method for preventing or treating coccidiosis comprising administering the composition to a chicken.
  • Feed composition, pharmaceutical composition, coccidiosis, prevention, and treatment are as described above.
  • 'Anetol' Compound of Formula 1 (hereinafter referred to as 'Anetol') was purchased from Sigma-Aldrich (W208604-1KG-K), soybean oil was used as a general cooking oil, Charcoal (Catalog number: 2530-1405).
  • Comparative Example 1 was prepared by diluting 20 ml of anetol in 180 ml of ethanol. The concentration of anethol in the Comparative Example 1 formulation was 2000 ppm.
  • Example 1 The concentration of anethol in the formulation was 2000 ppm.
  • Example 2 It was prepared in the same manner as in Example 1 except that 20 ml of anetol was changed to 10 ml, and 20 g of charcoal was changed to 50 g.
  • Example 2 The concentration of anethol in the formulation was 1000 ppm.
  • Example 1 The formulation of Example 1 was dipped in 20 ml of soybean oil and then taken out to prepare the formulation of Example 3.
  • Example 3 The concentration of anethol in the formulation was 2000 ppm.
  • Example 2 Anetol (ml) 0 20 0 20 10 20 Charcoal (g) 0 0 50 20 50 20 Soybean oil (ml) 0 0 0 0 0 50 ppm 0 0 0 2000 1000 2000 Anetol / Feed (ml / g) 0 0.2% 0 0.2% 0.1% 0.2%
  • the oocytes of purely differentiated Amerian Tenella (GenBank: FJ447468.1) were infected orally with 10,000-day-old chicks at 10,000 chickens per chicken, and feces were collected once a day from 4 days after infection.
  • the number of oocytes per gram of feces using the EPG counter is measured and shown in Table 2 and FIG. 1.
  • the sancho used in the present invention has an insecticidal effect against Aimerian tenella, and therefore, has a therapeutic effect of coccidiosis by Aimerian tenella.
  • the acidic pulverized fraction was fractionated using the initial solvent ethanol, and the insecticidal experiment was continuously conducted to confirm the therapeutic effect of coccidium, and the insecticidal effect of Ayeria tenella was confirmed to be due to anetol.
  • the feed containing the composition of Comparative Example 1 was fed to chickens.
  • the feeding method and the measurement of the number of eggs were carried out in the same manner as in Experiment 1, the results of the measured number of eggs are shown in Table 3 and FIG.
  • Control Comparative Example 1 5 days of infection 0.5 0 6 days of infection 109.25 61.5 7 days of infection 7550 8400 8 days of infection 2975 2285 9 days of infection 850 1200 10 days of infection 124 135
  • Example 2 Example 3 6 days of infection 0 ⁇ 0.00 0 ⁇ 0.00 0 ⁇ 0.00 0 ⁇ 0.00 0 ⁇ 0.00 0 ⁇ 0.00 7 days of infection 6875 ⁇ 377.49 11875 ⁇ 953.50 4250 ⁇ 680.68 925 ⁇ 411.30 1350 ⁇ 238.04 8 days of infection 3025 ⁇ 573.73 2300 ⁇ 110.45 995 ⁇ 106.61 817.5 ⁇ 86.17 460 ⁇ 111.06 9 days of infection 810 ⁇ 64.81 135 ⁇ 19.15 228 ⁇ 21.68 479.5 ⁇ 17.97 191.25 ⁇ 8.02
  • Example 1 including anetol and charcoal, it showed an excellent insecticidal effect compared to the control, which is difficult to confirm the insecticidal effect when anetol alone in Comparative Example 1 Contrasted. That is, when the adsorbents are mixed and administered together during the administration of anetol, it can be seen that the unstable anethol can be transported in a stabilized state by adsorbing the adsorbent, through which the insecticidal effect is excellent.
  • Example 1 an excellent insecticidal effect was also observed in Example 1, in which the amount of charcoal was increased and the amount of anetol was reduced. I could see that.
  • Example 1 the amount of anetol and charcoal was the same as in Example 1, while the third Example including the additional soybean oil showed the lowest number of eggs on the 8th and 9th days of infection, and especially soybean oil compared to Example 1. By doing so, the insecticidal effect was found to be much increased. This suggests that soybean oil acts as a coating agent of anetol to increase the stability, thereby increasing the insecticidal effect.
  • the amount of anetol was adjusted to 1 ml, 5 ml, and 10 ml in Example 1, respectively, to prepare the preparations having the concentration of anetol at 100, 500, and 1000 ppm, respectively. Prepared. This was administered with feed in the same manner as in Experimental Example 1 to measure the number of eggs per gram of chicken feces 7 days of infection, the results are shown in Table 5 and FIG.

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Abstract

La présente invention concerne une composition d'aliments pour animaux permettant de prévenir ou de traiter la coccidiose ; une composition pharmaceutique ; une composition protozocide d'Eimeria tenella ; et une méthode de prévention ou de traitement de la coccidiose, comprenant de l'anéthol ou un sel pharmaceutiquement acceptable de celui-ci, et un adsorbant poreux utilisés comme principes actifs. La présente invention concerne également une composition d'aliments pour animaux permettant de prévenir ou de traiter la coccidiose ; une composition pharmaceutique ; et une méthode de prévention ou de traitement de la coccidiose, comprenant du poivre du Sichuan ou des extraits de poivre du Sichuan utilisé comme principe actif. La composition de la présente invention comprenant de l'anéthol et un adsorbant utilisés comme principes actifs ; du poivre du Sichuan ; et des extraits de poivre du Sichuan est efficace pour la prévention ou le traitement de la coccidiose, et peut ainsi être largement utilisée pour une composition d'aliments pour animaux, une composition pharmaceutique, une composition protozocide, et une méthode thérapeutique, etc., pour la prévention ou le traitement de la coccidiose.
PCT/KR2018/000613 2017-01-12 2018-01-12 Composition pour la prévention ou le traitement de la coccidiose, son procédé de production et utilisation associée WO2018131930A1 (fr)

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JP3628022B2 (ja) * 1994-10-26 2005-03-09 クリナ ソシエテ アノニム 家禽飼料用添加剤組成物
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JP5829003B2 (ja) * 2006-05-17 2015-12-09 ダ・ボルテラ 単独でのまたは分解性分子と併用での吸着剤の部位特異的腸内送達
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