WO2018101223A1 - Micro-organisme ayant la capacité de lutter contre des maladies des plantes - Google Patents

Micro-organisme ayant la capacité de lutter contre des maladies des plantes Download PDF

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WO2018101223A1
WO2018101223A1 PCT/JP2017/042474 JP2017042474W WO2018101223A1 WO 2018101223 A1 WO2018101223 A1 WO 2018101223A1 JP 2017042474 W JP2017042474 W JP 2017042474W WO 2018101223 A1 WO2018101223 A1 WO 2018101223A1
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disease
strain
plant
microorganism
pseudomonas
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PCT/JP2017/042474
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English (en)
Japanese (ja)
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庸二 池田
浩靖 細川
原本　雅昇
茂雄 山田
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日本曹達株式会社
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Priority to JP2018554141A priority Critical patent/JP6748735B2/ja
Publication of WO2018101223A1 publication Critical patent/WO2018101223A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Definitions

  • the present invention relates to a microorganism having an ability to control plant diseases.
  • Bio pesticides have advantages such as extremely low environmental pollution, harmony with the ecosystem, and excellent control effect compared to conventional synthetic fungicides.
  • a microbial pesticide used for controlling bacterial diseases of agricultural and horticultural crops for example, Pseudomonas rhodesiae or a mutant thereof having an ability to control plant diseases is known (Patent Document 1).
  • Patent Document 1 an agricultural and horticultural fungicide composition containing Pseudomonas sp.
  • Patent Document 2 which is particularly used for the control of rice seed infectious bacterial diseases of rice blast and bacterial seedling Has been developed (Patent Document 2), and in Patent Document 3, Pseudomonas fluorescens G7090 strain has a control effect against the rot disease (Pseudomonas cichorii) which is a bacterial disease of lettuce.
  • Pseudomonas azotoformans F30A strain is described in Patent Document 4, and it is described in Non-Patent Document 1 that S4 strain is active in rice sapling disease.
  • Non-patent document 2 describes that the GC-B19 strain has activity against cucumber anthracnose, while the NBRC12693 strain is a non-patent document.
  • Pseudomonas azotoformans is a fungus. It is described as an example. Of these Pseudomonas azotoformans, only two strains, S4 and GC-B19 strains, are known which have the ability to control plant diseases.
  • these microbial pesticides are generally limited to only a small part of bacterial diseases and fungal diseases, and are not very effective against other bacterial diseases and most fungal diseases. I can't expect it. For this reason, the development of a microbial pesticide that is effective for a wider variety of diseases, practical as a pesticide, and has high activity is desired.
  • An object of the present invention is to provide a novel microorganism that has a low environmental load, has a very low possibility of causing resistant pathogens, and has an excellent control ability against various types of plant diseases. is there.
  • the present invention provides a novel microorganism that not only has a practical effect on filamentous fungal diseases but also has a practical and high control effect as an agrochemical against both bacterial diseases and fungal diseases.
  • the present inventors collected microorganisms from various plants, cultured and isolated them, and searched for highly active microorganisms. As a result, the present inventors have found a microorganism having a high control effect against various plant diseases such as lettuce soft rot. When the microbiological properties of this microorganism and the base sequence of the 16S rDNA gene were examined, it was found to be a new strain of Pseudomonas azotoformans.
  • the present invention relates to the following inventions.
  • a method for controlling plant diseases comprising treating Pseudomonas azotoformans W-14-1 strain NITE BP-02371, or a mutant strain thereof, to a plant and / or a cultivated soil of the plant.
  • Pseudomonas azotoformans W-14-1 strain NITE BP-02371, or a mutant thereof to a plant and / or a cultivated soil of the plant.
  • Pseudomonas azotoformans W-14-1 strain NITE BP-02371, or a mutant thereof and a plant disease control agent containing a surfactant.
  • Pseudomonas azotoformans W-14-1 strain NITE BP-02371 or a mutant thereof is 1 part by mass, and the blending ratio of the surfactant is 0.01 to 30 parts by mass.
  • the microorganism of the present invention has a low environmental load, has a very low possibility of causing resistant pathogens, and has a long-term control ability against various plant diseases. Moreover, the control effect can be shown to both a bacterial disease and a filamentous fungal disease.
  • FIG. 2 is a diagram showing a phylogenetic tree of Pseudomonas azotoformans W-14-1 strain (Pseudomonas azotoformans NITE BP-02371) of the present invention created based on the nucleotide sequence of the 16S rDNA gene.
  • the microorganism of the present invention is a Pseudomonas azotoformans W-14-1 strain having the ability to control plant diseases, and naturally occurring from the strain without any artificial manipulation. Or a mutant strain obtained by an artificial mutagenesis operation from the strain, which has an ability to control plant diseases. “Having control ability against plant diseases” means having an antagonism against pathogenic bacteria of any plant disease.
  • the microorganism of the present invention prevents or cures a plant disease caused by a pathogenic fungus by exerting an antagonistic action against the pathogenic fungus of the plant disease, and is particularly excellent in the effect of preventing a plant disease.
  • Preventing plant diseases means that the microorganisms of the present invention are treated under the same suitable conditions except that the plant pathogens that are not infected with plant diseases or that have no disease symptoms are treated with the microorganisms of the present invention. When cultivated, it means that the degree of the disease of the plant treated with the microorganism of the present invention is lower than that of the plant not treated with the microorganism of the present invention. “Healing plant diseases” means that the plant of the present invention is cultivated under the same suitable conditions except that the microorganisms of the present invention are treated except that the disease symptoms are manifested by infection with the plant disease pathogen.
  • the degree of the disease in the plant which processed the microorganism of this invention is lower than the plant which did not process the microorganism of this. “The degree of disease is low” means, for example, that the disease severity (or rate) is low and the control value is greater than zero.
  • the control value is preferably a larger value, excellent if it is 30 or more, more excellent if it is 50 or more, and particularly excellent if it is 60 or 70 or more.
  • the mycological properties of Pseudomonas azotoformans W-14-1 strain (hereinafter referred to as “the present microorganism”), which is the microorganism of the present invention, are as follows.
  • This microorganism is a Gram-negative bacilli and does not form spores.
  • the total length of the cells is 1.5 to 2.5 ⁇ m, the total width is about 0.8 ⁇ m, and motility is observed. Roughly rough colonies appear on the standard agar medium, and fluorescent dyes are produced on the King B medium.
  • D-glucose is positive
  • L-arabinose is positive
  • D-mannose is positive
  • D-mannitol is positive
  • N- Acetyl-D-glucosamine positive maltose negative
  • potassium gluconate positive potassium gluconate positive
  • n-capric acid positive adipic acid negative
  • DL-malic acid positive sodium citrate positive
  • phenyl acetate negative sucrose Is positive
  • trehalose is positive
  • ribitol (adnit) is positive
  • sorbitol is positive
  • butyric acid is negative
  • propionic acid is positive
  • propylene glycol is negative.
  • the measuring method of each item is not particularly limited, and a generally known method can be used.
  • a generally known method can be used.
  • the method described in Patent Document 1 can be used.
  • This microorganism can be obtained by isolating the 16S rDNA gene sequence (see SEQ ID NO: 3) and / or the above-mentioned mycological properties of the strain as indicators, and then culturing the cells.
  • This microorganism is a microbial cell of any form (for example, dormant cells) indicated by viable bacteria of Pseudomonas azotoformans W-14-1, including vegetative cells of Pseudomonas azotoformans W-14-1. It may be.
  • the method for culturing the microorganism is not particularly limited.
  • a method of shaking and stirring at 20 to 35 ° C. using various liquid media obtained by removing agar from the agar medium there can be mentioned.
  • Plant disease This microorganism can be used for controlling plant diseases derived from bacteria belonging to obligate aerobic bacteria, microaerobic bacteria, facultative anaerobic bacteria, etc. Can be used to control plant diseases derived from filamentous fungi belonging to algal fungi (Oomycetes), Ascomycetes, incomplete fungi (Deuteromycetes), basidiomycetes, zygomycetes (Zygomycetes), etc. .
  • this microorganism is soft rot, rot disease, spot bacterial disease, leafy leaf bacterial disease, itch disease, perforated bacterial disease, black spot bacterial disease, brown spot bacterial disease, stem wilt bacterial disease, rice wilt bacterial disease, seedling Bacterial diseases such as stand-off bacterial disease, bacterial wilt disease, white leaf blight disease, black rot disease, burn disease, Gray mold disease, asbestos disease, mycorrhizal disease, brown spot disease, black star disease, spotted leaf disease, black spot disease, summer plague disease, ring mold disease, leaf mold disease, subtle mold disease, scab disease, anthrax disease, udon It has the ability to control fungal diseases such as mildew, rust, sunspot, plague and downy mildew. However, the target of control by this microorganism is not limited to these.
  • plant disease pathogenic microbe
  • sugar beet brown spot disease (Cercospora beticola), black root disease (Aphanomyces cochlioides), root rot (Thanatephorus cucumeris), leaf rot (Thanatephorus cucumeris), etc.
  • Rust disease Puccinia arachidis
  • withering disease Pythium debaryanum
  • rust spot disease Alternaria alternata
  • white silk disease Sclerotium rolfsii
  • cucumber: powdery mildew Sphaerotheca fuliginea
  • Downy mildew Pseudoperonospora cubensis
  • vine blight Mycosphaerella melonis
  • vine split disease Fusarium oxysporum
  • mycorrhizal disease Sclerotinia sclerotiorum
  • gray mold disease Botrytis cinerea
  • anthracnose disease Coldletotrichum orbiculare
  • black star disease Cladosporium cucumerinum
  • brown spot Corynespora cassiicola
  • seedling blight Pythium debaryanum, Rhizoctonia solani Kuhn
  • Tomato Gray mold disease (Botrytis cinerea), leaf mold disease (Cladosporium fulvum), plague (Phytophthora infestans), half body wilt disease (Verticillium albo-atrum, Verticillium dahliae), powdery mildew (Oidium neolycopers) , Ring rot (Alternaria solani), mildew (Pseudocercospora fuligena), etc.
  • Eggplant gray mold (Botrytis cinerea), black blight (Corynespora melongenae), powdery mildew (Erysiphe cichoracearum), mildew (Mycovellosiella nattras) , Sclerotinia sclerotiorum, Verticillium dahliae, Phomopsis vexans, etc.
  • Strawberries Gray mold disease (Botrytis cinerea), powdery mildew (Sphaerotheca humuli), anthracnose (Colletotrichum acutatum) Colletotrichum fragariae, plague (Phytophthora cactorum), soft rot (Rhizopus stolonifer), yellow rot (Fusarium oxysporum), wilt (Verticillium dahliae), etc.
  • Onions Color rot (Botrytis allii), gray mold (Botrytis cinerea), white leaf blight (Botrytis squamosa), downy mildew (Peronospora destructor), white plague (Phytophthora porri), cabbage: root-knot disease (Plasmodiophora brassicae), Soft rot (Erwinia carotovora), black rot (Xanthomonas campesrtis pv. Campestris), black spot bacterial disease (Pseudomonas syringae pv. Maculicola, P. s. Pv.
  • Apples powdery mildew (Podosphaera leucotricha), black spot disease (Venturia inaequalis), monilinia disease (Monilinia mali), black spot disease (Mycosphaerella pomi), rot disease (Valsa mali), spotted leaf disease (Alternaria mali), red star disease (Gymnosporang) yamadae), ring rot (Botryosphaeria berengeriana), anthracnose (Glomerella cingulata, Colletotrichum acutatum), brown spot (Diplocarpon mali), soot spot (Zygophiala jamaicensis), soot spot (Gloeodes pomigena), purple coat rot (Helicobasidium mompa), gray mold disease (Botrytis cinerea), etc.
  • Ume Black rot (Cladosporium carpophilum), gray mold disease (Botrytis cinerea), gray star disease (Monilinia mumecola), etc.
  • Oysters powdery mildew (Phyllactinia kakicola), anthrax Diseases (Gloeosporium kaki), keratodeciduous leaf disease (Cercospora kaki), etc.
  • Grape rot (Monilinia kusanoi) Grapes: Gray mold ( Botrytis cinerea), powdery mildew (Uncinula necator), late rot (Glomerella cingulata, Colletotrichum acutatum), downy mildew (Plasmopara viticola), black scab (Elsinoe ampelina), brown spot (Pseudocercospora vitis), black rot (Guignardia bidwellii), white rot (Coniella castaneicola), rust (Phakopsora ampelopsidis), etc.
  • Citrus scab (Elsinoe fawcettii), blue mold (Penicillium italicum), green mold (Penicillium digitatum), gray mold (Botrytis cinerea), sunspot disease (Diaporthe citri), scab (Xanthomonas campestris pv.Citri), powdery mildew (Oidium sp.), Etc.
  • Rice blast (Pyricularia oryzae), blight (Rhizoctonia solani), idiot seedling (Gibberella fujikuroi), sesame leaf blight (Cochliobolus miyabeanus), seedling blight (Pythium graminicola , White leaf blight (Xanthomonas oryzae), seedling blight (Burkholderia plantarii), brown streak (Acidovorax avenae), blight blight (Burkholderia glumae), streak blight (Cercospora oryzae), rice leaf blight (Ustilaginoidea) virens), brown rice (Alternaria alternata, Curvul
  • Tobacco Sclerotinia sclerotiorum, powdery mildew (Erysiphe cichoracearum), plague ), Etc. Tulip: Gray mold disease (Botrytis cinerea), etc. Sunflower: Downy mildew (Plasmopara halstedii), Mycorrhizal disease (Sclerotinia sclerotiorum), etc.
  • Brown patch (Rhizoctonia solani), dollar spot (Sclerotinia homoeocarpa), rice blast (Pyricularia sp.), Red fire (Pythium aphanidermatum), anthrax Diseases (Colletotrichum graminicola) Orchardgrass: powdery mildew (Erysiphe graminis), etc.
  • Diseases Sterotinia sclerotiorum
  • anthracnose Colletotrichum truncatum
  • gray mold Botrytis cinerea
  • black scab Elsinoe glycines
  • black spot Diaporthe phaseolorum var. Sojae
  • Potato Phytophthora infestans, summer epidemic (Alternaria solani), black bruise (Thanatephorus cucumeris), half body wilt (Verticillium albo-atrum, V. dahliae, V. nigrescens), etc.
  • Banana Panama disease (Fusarium oxysporum), Sigatoka disease (Mycosphaerella fijiensis, M. musicola) Rapeseed: Sclerotinia sclerotiorum, Phoma lingam, Alternaria brassicae, etc.
  • Coffee Rust (Hemileia vastatrix), Anthrax (Colletotrichum coffeanum), brown eye disease (Cercospora coffeicola), etc.
  • Sugarcane brown rust (Puccinia melanocephala) etc. Diseases (Ustilago maydis), sesame leaf blight (Cochliobolus heterostrophus), soot rot (Setosphaeria turcica), etc. Cotton: seedling blight (Pythium sp.), Rust (Phakopsora gossypii), mildew (Mycosphaerella areola), Anthrax (Glomerella gossypii), etc.
  • the plant to which the present microorganism is applied is not particularly limited as long as the present microorganism is a plant that can exert a controlling ability.
  • the microorganism can be used for cereals, vegetables, root vegetables, potatoes, fruit trees, trees such as tea, coffee, cacao, grasses, grasses, and plants such as cotton.
  • plants belonging to the convolvulaceae family or the taro family can be mentioned.
  • plants belonging to the Brassicaceae family such as Chinese cabbage
  • plants belonging to the Compositae family such as lettuce
  • plants belonging to the solanaceous family such as potato
  • mandarin oranges such as lemon and navel
  • plants belonging to the family and plants belonging to the rose family such as pears.
  • the present microorganism can be applied to various parts of plants, for example, leaves, stems, patterns, flowers, strawberries, fruits, seeds, sprout, roots, tubers, tuberous roots, shoots, cuttings and the like.
  • improved varieties and varieties of these plants, cultivated varieties, and mutants, hybrids, and genetically modified organisms (GMO) can also be targeted.
  • This microorganism is used to control various diseases that occur in agro-horticultural crops including flower buds, turf, and grass. It can be used for application.
  • the plant disease control agent containing the microorganism is particularly suitable as long as it contains Pseudomonas azotoformans W-14-1 strain or a mutant thereof. Not limited.
  • the present control agent refers to a plant disease control agent containing the present microorganism.
  • the microorganism may be any of the microorganism itself, a suspension containing the microorganism, a culture solution containing the microorganism, a culture, a concentrate, a paste, a dried product, a diluted product, etc. Even if it is in a form, it can be used for the present control agent.
  • the present control agent can prevent or cure a plant disease caused by the pathogenic bacterium by causing the microorganism to antagonize the pathogenic bacterium of the plant disease.
  • this control agent can be used as a preventive agent for plant diseases or a therapeutic agent for plant diseases, it is particularly excellent in the effect as a preventive agent for plant diseases.
  • concentration of the present microorganism contained in the present control agent is not particularly limited as long as the effects of the present invention are not impaired.
  • concentration is 1
  • a preferred range is x10 2 to 1x10 11 cfu / ml, preferably 1 x 10 4 to 1 x 10 9 cfu / ml.
  • the present control agent may contain an optional component in addition to the present microorganism.
  • the optional component is not particularly limited as long as the effects of the present invention are not impaired, and examples thereof include a carrier, a surfactant, a dispersant, and an auxiliary agent. In particular, it is preferable to add at least a surfactant. Further, if necessary, an antioxidant, a colorant, a lubricant, an ultraviolet absorber, an antistatic agent, an antiseptic, and the like can be added.
  • chemical pesticides such as fungicides, insecticides, herbicides, growth regulators and the like can be mixed as long as they do not affect the present microorganism. In addition, chemical pesticides such as bactericides, insecticides, herbicides, growth regulators, etc. that affect this microorganism can be used with a spraying interval of several days.
  • the content of the microorganism is not particularly limited, but is 0.001 to 99 parts by weight, preferably 0.01 to 80 parts by weight, more preferably 0.1 to 100 parts by weight with respect to 100 parts by weight of the present control agent.
  • the amount is 70 parts by mass, more preferably 1 to 50 parts by mass.
  • Carriers include inorganic salts such as calcium carbonate, potassium chloride, sodium sulfate, calcium sulfate and ammonium sulfate; organic acids such as citric acid, malic acid and stearic acid; and salts thereof; sugars such as glucose, lactose and sucrose; alumina Solid carriers such as powder, silica gel, zeolite, hydroxyapatite, zirconium phosphate, titanium phosphate, titanium oxide, zinc oxide, hydrotalcite, kaolinite, montmorillonite, talc, clay, diatomaceous earth, bentonite, white carbon, kaolin, vermiculite Can be mentioned.
  • the content of the carrier is not particularly limited, but the mixing ratio of the carrier is 0.01 to 30 parts by weight, preferably 0.1 to 20 parts by weight with respect to 1 part by weight of the present microorganism. The amount is preferably 0.3 to 10 parts by mass.
  • the surfactant (which can also be used as a dispersant) is not particularly limited as long as it can be used in ordinary agricultural and horticultural preparations. Specifically, the following nonionic surfactants and anions are used. Surfactants, cationic surfactants, and amphoteric surfactants.
  • Nonionic surfactants include sorbitan fatty acid esters (C12-18), POE sorbitan fatty acid esters (C12-18), sugar ester type surfactants such as sucrose fatty acid esters; POE fatty acid esters (C12-18), Fatty acid ester type surfactants such as POE resin acid ester and POE fatty acid diester (C12-18); Alcohol type surfactants such as POE alkyl ether (C12-18); POE alkyl (C8-12) phenyl ether, POE dialkyl Alkylphenol type surfactants such as (C8-12) phenyl ether, POE alkyl (C8-12) phenyl ether formalin condensate; polyoxyethylene polyoxypropylene block polymer; alkyl (C12-18) polyoxyethylene poly Polyoxyethylene / polyoxypropylene block polymer type surfactants such as xylpropylene block polymer ether; alkylamine type surfactants such as POE alkylamine
  • anionic surfactant examples include alkyl sulfate (C12-18, Na, NH4, alkanolamine), POE alkyl ether sulfate (C12-18, Na, NH4, alkanolamine), POE alkylphenyl ether sulfate (C12-18, NH4, alkanolamine), POE benzyl (or styryl) phenyl (or phenylphenyl) ether sulfate (Na, NH4, alkanolamine), polyoxyethylene, polyoxypropylene block polymer sulfate (Na, NH4, alkanolamine) and other sulfates
  • Type surfactants paraffin (alkane) sulfonate (C12-22, Na, Ca, alkanolamine), AOS (C14-16, Na, alkanolamine) Dialkylsulfosuccinate (C8-12, Na, Ca, Mg), alkylbenzenesulf
  • phosphate type surfactants such as phosphatidylcholine / phosphatidylethanolimine (lecithin), alkyl (C8-12) phosphate, and the like.
  • Cationic surfactants include alkyltrimethylammonium chloride (C12-18), methyl polyoxyethylene alkylammonium chloride (C12-18), alkyl N-methylpyridium bromide (C12-18), mono- or dialkyl (C12-18) ammonium surfactants such as methylated ammonium chloride and alkyl (C12-18) pentamethylpropylenediamine dichloride; alkyldimethylbenzalkonium chloride (C12-18), benzethonium chloride (octylphenoxyethoxyethyldimethylbenzyl) Benzalkonium type surfactants such as ammonium chloride).
  • amphoteric surfactants include betaine surfactants such as dialkyl (C8-12) diaminoethylbetaine and alkyl (C12-18) dimethylbenzylbetaine; dialkyl (C8-12) diaminoethylglycine, alkyl (C12-18) Glycine-type surfactants such as dimethylbenzylglycine;
  • Surfactant and / or a dispersing agent can be used individually by 1 type or in mixture of 2 or more types.
  • the content of the surfactant and / or the dispersant is not particularly limited, but the mixing ratio of the surfactant is 0.01 to 30 parts by mass, preferably 0.
  • the amount is 1 to 20 parts by mass, and more preferably 0.3 to 10 parts by mass.
  • auxiliary agent examples include carboxymethyl cellulose, polyethylene glycol, gum arabic, polyvinyl pyrrolidone, starch and the like.
  • the form of the present control agent is not particularly limited, and forms that can be taken by ordinary agricultural and horticultural medicines, for example, powder (DP, Dustable Powder), wettable powder (WP, Wattable Powder), emulsion (EC, Emulsifiable Concentrate), Flowable agent (FL, flowable), suspension agent (SC, Suspension Concentrate), water solvent (SP, Water Soluble Powder), granule wettable powder (WG, Water Dispersible Granule), tablet (Tablet), granule (GR, Granule), SE agent (Suspo Emulsion), OD agent (Oil Dispersion), EW agent (Emulsion oil in water) and the like can be employed.
  • the preparation method to a formulation is not specifically limited, A well-known preparation method can be employ
  • Formulation 1 wettable powder
  • Higher alcohol sulfate 4 parts and alkylnaphthalene sulfonate 3 parts are uniformly mixed and finely pulverized to obtain a wettable powder of 40% active ingredient.
  • Formulation 2 Emulsion 30 parts of this microorganism 30 parts of xylene 30 parts of dimethylformamide and 7 parts of polyoxyethylene alkylallyl ether are mixed and dissolved to obtain an emulsion of 30% active ingredient.
  • Formulation 3 Granules
  • Talc 40 parts Clay 38 parts Bentonite 10 parts and 7 parts of sodium alkyl sulfate are uniformly mixed and finely pulverized, then granulated into granules having a diameter of 0.5 to 1.0 mm, and the active ingredient is 5%. Get the granules.
  • Formulation 4 Granules
  • This microorganism 5 parts Clay 73 parts Bentonite 20 parts Dioctylsulfosuccinate sodium salt 1 part and potassium phosphate 1 part are thoroughly pulverized and mixed. Get the granules.
  • Plant diseases and application plants that are the targets of the control agent are the same as the plant diseases and application plants that are the targets of the microorganisms.
  • a method for controlling plant diseases with the present control agent is particularly limited as long as it is a method of treating the control agent on a plant and / or cultivated soil of the plant. Instead, as in the case of using ordinary chemical pesticides, it can be appropriately selected depending on the type of plant disease, the type of applied plant and the like.
  • the present control agent may be treated to a plant, or the present control agent is mixed with soil (cultivation soil for plants), You may process this control agent to the cultivation soil of a plant by spraying or irrigating.
  • this control agent is treated on the cultivated soil of the plant, the plant may be planted after treating the soil with the control agent, or the plant may be treated with the present control agent after planting the plant in the soil.
  • the present pesticide can be installed near the air outlet of a blower that blows air inside the facility, and the pesticide can be sprayed together with the air sent from the air outlet.
  • the seeds and tubers of plants can be coated, dressed, soaked, etc. with this control agent, and can also be treated by soaking the roots of plant seedlings.
  • the present control agent When the present control agent is treated on a plant and / or cultivated soil of the plant, the present control agent can be used as it is, or can be used after diluted with an appropriate amount of water or the like. .
  • the amount of the control agent to be treated on the plant and / or the cultivated soil of the plant varies depending on the type of plant disease, the type of the applied plant, etc., and thus cannot be specified unconditionally.
  • the bacterial cell concentration it can be generally in the range of 1 ⁇ 10 2 to 1 ⁇ 10 11 cfu / ml, preferably 1 ⁇ 10 4 to 1 ⁇ 10 9 cfu / ml.
  • This control agent includes other components such as bactericides, insecticides, acaricides, nematicides, soil pesticides, plant regulators, synergists, fertilizers, soil improvers, animal feeds, etc. They can be mixed or mixed. By containing such other components, there may be a synergistic effect.
  • Nucleic acid biosynthesis inhibitors (a) RNA polymerase I inhibitor: benalaxyl, benalaxyl-M (benalaxyl-M), furaxyl (furalaxyl), metalaxyl, metalaxyl-M (metalaxyl-M), oxadixyl, clozylacon ), Off-race (ofurace); (b) adenosine deaminase inhibitors: bupirimate, dimethirimol, ethirimol; (c) DNA / RNA synthesis inhibitors: hymexazol, octhilinone; (d) DNA topoisomerase II inhibitor: oxolinic acid.
  • RNA polymerase I inhibitor benalaxyl, benalaxyl-M (benalaxyl-M), furaxyl (furalaxyl), metalaxyl, metalaxyl-M
  • Mitotic fission inhibitor and cell division inhibitor (a) ⁇ -tubulin polymerization inhibitors: benomyl, carbendazim, chlorfenazole, fuberidazole, thiabendazole, thiophanate, thiophanate-methyl , Dietophencarb, zoxamide, ethaboxam; (b) Cell division inhibitor: pencicuron (pencycuron); (c) Delocalization inhibitor of spectrin-like protein: fluopicolide.
  • Respiratory inhibitor (a) Complex I NADH oxidoreductase inhibitor: diflumetorim, tolfenpyrad; (b) Complex II succinate dehydrogenase inhibitors: benodanil, flutolanil, mepronil, isofetamid, fluopyram, fenfuram, furmecyclox , Carboxin, oxycarboxin, thifluzamide, benzovindiflupyr, bixafen, fluxapyroxad, furametpyr, isopyrazam, Penflufen, penthiopyrad, sedaxan, boscalid, pyraziflumid; (c) Complex III ubiquinol oxidase Qo inhibitors: azoxystrobin, coumoxystrobin, coumethoxystrobin, enoxastrobin, flufenoxystrobin , Picoxystrobin, pyraoxystrobin, pyraclostrobin, pyrametostrobin, triclop
  • Signaling inhibitor (a) Signaling inhibitors: quinoxyfen, proquinazid; (b) MAP / histidine kinase inhibitors in osmotic signal transduction: fenpiclonil, fludioxonil, clozolinate, iprodione, procymidone, vinclozolin.
  • Lipid and cell membrane synthesis inhibitors (a) Phospholipid biosynthesis, methyltransferase inhibitors: edifenphos, iprobenfos, pyrazophos, isoprothiolane; (b) lipid peroxidants: biphenyl, chloroneb, dichloran, quintozene, tecnazene, tolclofos-methyl, etridiazole; (c) Agents that act on the cell membrane: iodocarb, propamocarb, propamocarb-hydrochloride, propamocarb-fosetylate, prothiocarb; (d) Microorganisms that disrupt the cell membrane of pathogenic bacteria: Bacillus subtilis strain, Bacillus subtilis strain QST713 (bacillus subtilis strain QST713), Bacillus subtilis strain FZB24 strain (bacillus subtilis strain FZB24), Bacillus subtilis strain MBI600 strain MBI600 Bacillus subtilis
  • Cell membrane sterol biosynthesis inhibitors (a) Demethylation inhibitor at C14 position in sterol biosynthesis: triforine, pyrifenox, pyrisoxazole, fenarimol, flurprimidol, nuarimol, Imazalil, imazalil-sulphate, oxpoconazole fumarate, pefurazoate, prochloraz, triflumizole, viniconazole, azaconazole azaconazole), bitertanol, bromconazole, cyproconazole, diclobutrazol, difenoconazole, diniconazole, dinicoazole-M, epoxy Conazole (epoxyconazole), etaconazole (etaconazole), fenbuconazole (fenbuconazole), fluquinconazole (fluquinconazole), flusilazole (flusilazole), flutriafol, fluconazole (furcon
  • Trehalase inhibitor validamycin
  • chitin synthase inhibitors polyoxins, polyoxorim
  • Cellulose synthase inhibitors dimethomorph, flumorph, pyrimorph, benchthiavalicarb-isopropyl, iprovalicarb, tolprocarb, valifenalate ), Mandipropamid.
  • Melanin biosynthesis inhibitor (a) Reductase inhibitor of melanin biosynthesis: fthalide, pyroquilon, tricyclazole; (b) Dehydrase inhibitors of melanin biosynthesis: carpropamid, diclocymet, fenoxanil.
  • Host plant resistance inducer (a) Agents acting on the salicylic acid synthesis pathway: acibenzolar-S-methyl; (b) Others: probenazole, tiadinil, isothianil, laminarin, reynoutria sachalinensis extract.
  • Agents with unknown activity cymoxanil, fosetyl-aluminium, phosphoric acid (phosphate), tecloftalam, triazoxide, fursulfamide ( flusulfamide, diclomezine, metasulfocarb, cyflufenamid, metrafenone, pyriofenone, dodine, dodine free base (dodine free base), flutianil.
  • Agents with multiple action points copper (copper salt), bordeaux mixture, copper hydroxide, copper naphthalate, copper oxide , Copper oxychloride, copper sulfate, sulfur, sulfur product, calcium polysulfide, ferbam, mancozeb, maneb , Mancopper, metiram, polycarbamate, propineb, thiram, zineb, ziram, captan, captafol, phorpet (Folpet), chlorothalonil, dichlorfluanid, tolylfluanid, guazatine Iminoctadine acetate (iminoctadine triacetate), iminoctadine carbonochloridate tagine Alves Sil salt (iminoctadine trialbesilate), Anirajin (anilazine), dithianon (dithianon), Kinomechioneto (quinomethionate), Furuoruimido (fluoroimide).
  • insecticide acaricide, nematicide, soil insecticide, anthelmintic and the like that can be used in combination with or in combination with the present pesticide are shown below.
  • Acetylcholinesterase inhibitor (a) Carbamate series: alanicarb, aldicarb, bendiocarb, benfuracarb, butofacarb, butoxycarboxim, butoxycarboxim, carbaryl, carbofuran, Carbosulfan, ethiofencarb, fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, methomyl, oxamyl, pimimicarb (pir) ), Propoxur, thiodicarb, thiofanox, triazamate, trimethacarb, XMC, xylycarb, phenothioca Fenothiocarb, MIPC, MPMC, MTMC, aldoxycarb, aliyxycarb, aminocarb, bufencarb, cloethocarb, metam-sodium, promecarb );
  • GABA-agonist chloride channel antagonists acetoprole, chlordane, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, Camphechlor, heptachlor, dienochlor.
  • Sodium channel modulators acrinathrin, d-cis-trans allethrin, d-trans allethrin, bifenthrin, bioallethrin, biorethrin Allesrin s-cyclopentyl isomer, bioresmethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin ( ⁇ ) -cyhalothrin), gamma-cyhalothrin ( ⁇ -cyhalothrin), cypermethrin (cypermethrin), alpha-cypermethrin ( ⁇ -cypermethrin), beta-cypermethrin ( ⁇ -cypermethrin), theta-cypermethrin ( ⁇ -cypermethrin), zeta -Cypermethrin,
  • Nicotinic acetylcholine receptor agonists acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, nithiazine, thiacloprid, thiamethoxam, thiamethoxam Sulfoxaflor, nicotine, flupradifurone.
  • Nicotinic acetylcholine receptor allosteric modulators spinetoram, spinosad.
  • Chloride channel activator Abamectin, emamectin benzoate, empiectin, lepimectin, milbemectin, ivermectin, seramectin, doramectin, eprinomectin (Eprinomectin), moxidectin, milbemycin, milbemycin oxime, nemadectin.
  • Juvenile hormone-like substances hydroprene, kinoprene, metoprene, phenoxycarb, pyriproxyfen, diofenolan, epofenonane, triprene ).
  • Non-specific inhibitors methyl bromide, chloropicrin, sulfuryl fluoride, borax, tartar emetic.
  • Homoptera selective feeding inhibitors flonicamid, pymetrozine, pyrifluquinazon.
  • Tick growth inhibitor clofentezine, diflovidazin, hexythiazox, etoxazole.
  • Microbial-derived insect midgut lining disruptors Bacillus thuringiensis subsp. Israelensi, bacillus sphaericus, Bacillus thuringiensis subsp. Aizawai , Bacillus thuringiensis subsp. Kurstaki, Bacillus thuringiensis subsp.
  • Nicotinic acetylcholine receptor channel blocker bensultap, cartap hydrochloride, nereistoxin, thiosultap-sodium, thiocyclam.
  • Chitin synthesis inhibitors bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, Lufenuron, novaluron, noviflumuron, teflubenzuron, triflumuron, buprofezin, fluazuron.
  • Diptera molting agent Cyromazine.
  • Molting hormone receptor agonists chromafenozide, halofenozide, methoxyfenozide, tebufenozide.
  • Octopamine receptor agonists amitraz, demiditraz, chlordimeform.
  • Mitochondrial electron transport system complex III inhibitor acequinocyl, fluacrypyrim, hydramethylnon.
  • Mitochondrial electron transport complex I inhibitor fenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad, tolfenpyrad, rotenone.
  • Voltage-gated sodium channel blocker indoxacarb, metaflumizone.
  • Acetyl CoA carboxylase inhibitor spirodiclofen, spiromesifen, spirotetramat.
  • Mitochondrial electron transport system complex IV inhibitor aluminum phosphide, calcium phosphide, phosphine, zinc phosphide, cyanide.
  • Mitochondrial electron transport system complex II inhibitors cyenopyrafen, cyflumetofen, piflubumide.
  • Ryanodine receptor modulators chlorantraniliprole, cyantraniliprole, flubendiamide, cyclaniliprole, tetraniliprole.
  • Anthelmintic (a) benzimidazole series: fenbendazole, albendazole, triclabendazole, oxibendazole, mebendazole, oxfendazole, perbendazole ( parbendazole), flubenzazole, febantel, netobimin, thiophanate, thiabendazole, cambendazole; (b) Salicylanilide series: closantel, oxyclozanide, rafoxanide, niclosamide; (c) substituted phenols: nitroxinil, nitroscanate; (d) Pyrimidine series: pyrantel, morantel; (e) Imidazothiazole series: levamisole, tetramisole; (f) Tetrahydropyrimidine series: praziquantel, epsiprantel; (g) Other anthelmintic drugs: cyclodiene, ryania, clorsulon
  • plant regulators that can be used in combination or in combination with the present control agent are shown below. Abscisic acid, kinetin, benzylaminopurine, 1,3-diphenylurea, forchlorfenuron, thidiazuron, chlorfenuron ( chlorfenuron, dihydrozeatin, gibberellin A, gibberellin A4, gibberellin A7, gibberellin A3, 1-methylcyclopropane, N -N-acetyl aminoethoxyvinyl glycine (aka glycine (aviglycine)), aminooxyacetate, silver nitrate, cobalt chloride, IAA, 4-CPA, cloprop (Cloprop), 2,4-D, MCPB, indole-3- Acid (indole-3-butyrate), dichlorprop, phenothiol, 1-naphthyl acetamide, ethychlozate, cloxyfonac, male
  • the number of sprays of the present control agent can be appropriately selected depending on the type of plant disease, the type of applied plant, the degree of disease, and the like.
  • the present control agent can be used mainly for agricultural purposes, but can also be used for purposes other than agricultural purposes.
  • agricultural uses include seed treatment, seed disinfection, rice fields, spraying on soil such as fields, and spraying on plants such as vegetables, fruits and rice.
  • examples thereof include antifungal agents for walls of living rooms, water quality improvers such as ponds, pools, and cooling towers, and sludge treatment agents such as sludge.
  • This control agent can be used without any particular limitation on the spraying method, but it is effective by diluting in water and spraying it, spraying the solid agent on the soil as it is, putting the solid agent in water and releasing it slowly.
  • the method etc. which can exhibit can be illustrated.
  • Example 1 Isolation and identification of bacteria Separation of candidate strains and selection of strains having plant disease control ability
  • Chinese cabbage leaves collected in the field were homogenized in a small amount of sterilized water, and the resulting suspension was added to a standard agar medium (casein peptone 0.5% (w / V), yeast extract 0.25% (w / v), glucose 0.1% (w / v), agar 1.5% (w / v), pH 6.9 to 7.1 after sterilization) ( Nissui Pharmaceutical Co., Ltd.) was smeared and cultured at 25 ° C. for 2 days. A plurality of grown single microbial colonies were collected and used as candidate strains.
  • a standard agar medium casein peptone 0.5% (w / V), yeast extract 0.25% (w / v), glucose 0.1% (w / v), agar 1.5% (w / v), pH 6.9 to 7.1 after sterilization
  • Nissui Pharmaceutical Co., Ltd. was
  • the treatment liquid was sprayed on the cut-out opening of the cut leaves in the middle part of the lettuce.
  • the cut mouth was inoculated.
  • the severity of the disease was investigated according to the following criteria, and the disease severity and the control value were calculated based on the following formulas 1 and 2.
  • Criterion 0 No lesion criterion 1: Lesions are less than 10% of the midribs Criterion 2: Lesions are less than 10-50% of the midribs Criterion 3: Lesions are less than 50-75% of the midribs Criterion 4: Lesions are More than 75% of the middle collar
  • Criterion 0 No disease Criterion 1: crisis is observed in some of the outer leaves 2: Crisis is observed in some of the outer and head leaves 3: Crisis is observed in most of the head leaves
  • genomic DNA was isolated from the W-14-1 strain according to a conventional method. Using the obtained genomic DNA as a template, PCR amplification was performed using 27F primer (SEQ ID NO: 1) and 1510R primer (SEQ ID NO: 2) often used for 16S rRNA gene amplification. The obtained PCR product was sequenced and the nucleotide sequence was decoded.
  • nucleotide sequence of the 16S rRNA gene of the W-14-1 strain is shown in SEQ ID NO: 3.
  • the W-14-1 strain is a bacterium belonging to the Pseudomonas fluorescens group, and is further included in a clade composed of Pseudomonas gesardi, Pseudomonas rivanensis, Pseudomonas cinesansa, Pseudomonas musciduens and Pseudomonas azotoformans. did. Among them, the homology was particularly high with Pseudomonas azotoformans.
  • the mycological properties of W-14-1 strain are as follows.
  • the gram-negative bacilli do not form spores, the total length of the cells is 1.5 to 2.5 ⁇ m, the total width is about 0.8 ⁇ m, and motility is observed. Roughly rough colonies appear on the standard agar medium, and fluorescent dyes are produced on the King B medium. No growth at 41 ° C., catalase activity is positive, OF medium test is oxidation, nitrate reduction is negative, indole production is negative, urease activity is negative, gelatin degradation is positive, ⁇ -galactosidase activity is negative, ⁇ -glucosidase activity is negative.
  • the test was conducted according to the attached protocol.
  • the oxidase activity was tested using Oxidase Reagent (Nihon Biomeryu Co., Ltd.), and the catalase activity was tested using ID color Catalase (Nihon Biomelieu Co., Ltd.).
  • the King B medium used for the King B medium test was performed using King B medium manufactured by Eiken Chemical Co., Ltd. About Gram dyeing
  • the strain is streaked on a flat plate of 5% sucrose-added normal agar medium and cultured at 25 ° C for 3 days to form white viscous large dome-shaped colonies was judged positive.
  • Potato tuber rot test tobacco hypersensitive reaction test, starch assimilation test, and assimilation of carbon compounds such as sugars and organic acids can be measured by known methods.
  • the method described in Patent Document 1 can be used.
  • Pseudomonas fluorescens which is a related species of both species, is known to exhibit pathogenicity against tomato and cause tomato stem wilt bacterial disease.
  • the W-14-1 strain is cultured in a liquid medium until the A600 (absorbance at a wavelength of 600 nm) reaches 1.0, and the culture solution is sprayed and inoculated on the main stem and the petiole part centering on the petiole base of the tomato seedling. did. After cultivating for 7 days, the seedlings were observed, and no symptoms of tomato stem wilt were observed.
  • the W-14-1 strain differs from the Pseudomonas ribanensis CIP105460 strain in that the morphological characteristics of the colony side, periphery, and surface, nitrate reducing ability, gelatin degradability, and arginic acid degradability , Utilization of sucrose, utilization of propionic acid, and utilization of propylene glycol.
  • the W-14-1 strain differs from Pseudomonas azotoformans NBRC12693 only in the presence or absence of nitrate reducing ability, gelatin degradability, adipic acid assimilation ability, and propylene glycol assimilation ability. It was. From the above results, the W-14-1 strain was identified as Pseudomonas azotoformans because it was more homologous to Pseudomonas azotoformans than Pseudomonas rivanensis.
  • the wettable powder composition is a drug containing Pseudomonas azotoformans W-14-1 strain as an active ingredient.
  • Criterion 0 No attack Criterion 1: crisis is observed in a part of the outer leaf 2: crisis is observed in a part of the outer leaf and the head leaf 3: Damage on the major portion of the head leaf or more Is accepted
  • Citrus canker disease (bacterial disease) control effect test field test
  • a treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water. The treatment solution was sprayed over the entire navel tree. The spraying process was carried out three times in the initial stage of leaf spreading and twice every two weeks thereafter.
  • the severity of citrus scab on the navel leaf was investigated according to the following criteria, and the severity was determined based on the following formulas 7 and 8. And the control value was calculated. As a result, the control value was 62.5.
  • Criteria 0 No lesion criteria Criteria 1: 1 to 3 lesions Criteria 3: 4 to 10 lesions Criteria 5: 11 to 20 lesions Criteria 7: 21 or more lesions
  • Criterion 0 No attack Criterion 1: crisis on some outer leaves 2: Crisis on most outer leaves 3: Crisis on outer and inner leaves 4: Crisis on head leaves
  • Tomato gray mold disease (filamentous fungal disease) control effect test field test
  • a treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water. The treatment liquid was sprayed on tomatoes planted in the field. The spraying process was performed four times at one week intervals.
  • the pathogen of tomato gray mold Botrytis cinerea
  • Five surveys were conducted, including the final survey 6 days after the fourth spraying, and all young fruits were examined for the presence of disease.
  • the total number of fruits was counted at the time of the final investigation, and the disease rate and the control value were calculated based on the following formulas 11 and 12. As a result, the control value was 89.9.
  • Pear black spot disease filamentous fungal disease
  • field test A treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water. The treatment solution was sprayed over the whole pear tree that had been planted in the field. The spraying treatment was performed twice, at the beginning of flowering and immediately after the fall. Two days after the second spraying, the presence or absence of pear black spot disease (pathogen Alternaria kikuchiana) on pear leaves was investigated, and the diseased leaf rate and control value were calculated based on Equation 13 and Equation 14 below. did. As a result, the control value was 60.5.
  • Citrus gray mold disease (filamentous fungal disease) control effect test field test
  • a treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water. The treatment solution was sprayed over the whole mandarin tree planted in the field. The spraying process was carried out twice in the early stage of leaf opening and the beginning of flowering.
  • the pathogen of citrus gray mold Botrytis cinerea
  • pumpkin fruits having their spores formed in the 7-minute blooming period were arranged in the field.
  • the presence or absence of disease on the mandarin flower was investigated, and the disease flower rate and control value were calculated based on the following formulas 15 and 16. As a result, the control value was 70.3.
  • Citrus scab (filamentous fungal disease) control effect test field test
  • a treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water.
  • the treatment solution was sprayed over the whole mandarin tree planted in the field.
  • the spraying process was carried out three times, at the early stage of leaf spreading, at the beginning of flowering and at the time of valve closing.
  • Thirteen days after the third application the incidence of citrus scab on the mandarin orange leaf (pathogenic fungus Elsinoe fawcettii) was investigated according to the following criteria.
  • the control value was calculated. As a result, the control value was 68.8.
  • Criteria 0 No lesion criteria Criteria 1: 1-5 lesions Criteria: 6-20 lesions Criteria 5: 21-50 lesions Criteria 7: 51 or more lesions
  • Potato Summer Pest (Fill Fungus Disease) Control Effect Test Field Test
  • a treatment solution was prepared by diluting W-14-1 strain wettable powder 1000 times with water. The treatment solution was sprayed on the potato planted in the field. The test was performed twice at 10 day intervals. Four days after the second spraying, we investigated the degree of potato leaf blight on potato leaves (pathogen Alternaria solani) on the basis of the following criteria, and based on the following formulas 19 and 20, the severity and control value Was calculated. As a result, the control value was 52.2.
  • Criteria 0 No lesion on the leaf Criteria 1: The lesion area is less than 5% of the leaf area Criteria 2: The lesion area is less than 5 to 25% of the leaf area Criteria 3: The lesion area is 25 to 25% of the leaf area Less than 50% criterion 4: lesion area is 50% or more of leaf area
  • this microorganism is effective as a bacterial disease at least for lettuce soft rot, Chinese cabbage soft rot, lettuce rot, citrus canker, lettuce spot bacterial disease, and as a fungal disease, at least tomato gray mold disease.
  • a fungal disease at least tomato gray mold disease.
  • Pear black spot, citrus gray mold, citrus scab, and potato summer plague were found to be effective.
  • both the bacterial disease and the filamentous fungal disease have a high control effect.
  • Fungi or mildew (bacteria) and scab (filamentous fungi), lettuce, soft rot (bacteria) and gray mold (filamentous fungi), rot (bacteria) and gray mold (filamentous fungi), Alternatively, it has been found that the present microorganism alone can simultaneously exert an effect on spot bacterial disease (bacteria) and gray mold disease (filamentous fungus).

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Abstract

La présente invention aborde le problème consistant à fournir de nouveaux micro-organismes dont l'impact sur l'environnement est limité, ont une très faible probabilité de conduire à des organismes pathogènes résistants, et ont une exceptionnelle capacité à lutter contre une variété plus large de maladies des plantes. En particulier, la présente invention aborde le problème consistant à fournir de nouveaux micro-organismes aptes à présenter un effet de lutte sur non seulement des maladies fongiques filamenteuses mais également sur des maladies bactériennes. Ces nouveaux micro-organismes sont la souche de Pseudomonas azotoformans W-14-1 NITE BP-02371 ou une souche mutante de ce dernier et présentent un effet de lutte élevé contre diverses maladies des plantes, dont la pourriture molle de la laitue.
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CN112674083B (zh) * 2019-10-17 2022-11-01 山东潍坊润丰化工股份有限公司 一种联苯菊酯和氟苯吡菌胺的可分散油悬浮剂及其制备方法和应用
CN111690629B (zh) * 2020-05-29 2022-04-19 浙江工业大学 一种内切葡聚糖苷酶突变体、基因、工程菌及其应用
CN112940964B (zh) * 2021-01-29 2023-05-09 西南大学 一种喀斯特槽谷区石漠化土壤改良微生物菌剂及其制备与应用
CN115537363B (zh) * 2022-11-24 2023-03-10 云南省农业科学院农业环境资源研究所 枯草芽孢杆菌、菌剂和生物制剂及其应用

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US11170872B2 (en) 2019-11-05 2021-11-09 Apeel Technology, Inc. Prediction of latent infection in plant products
CN117063924A (zh) * 2023-10-13 2023-11-17 西南林业大学 一种缓释长效三七用杀菌剂及其制备方法
CN117063924B (zh) * 2023-10-13 2023-12-15 西南林业大学 一种缓释长效三七用杀菌剂及其制备方法

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