WO2018066940A1 - Module de couplage d'endoscope multiplex - Google Patents

Module de couplage d'endoscope multiplex Download PDF

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Publication number
WO2018066940A1
WO2018066940A1 PCT/KR2017/011004 KR2017011004W WO2018066940A1 WO 2018066940 A1 WO2018066940 A1 WO 2018066940A1 KR 2017011004 W KR2017011004 W KR 2017011004W WO 2018066940 A1 WO2018066940 A1 WO 2018066940A1
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WO
WIPO (PCT)
Prior art keywords
adapter
coupling
light
laser light
objective lens
Prior art date
Application number
PCT/KR2017/011004
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English (en)
Korean (ko)
Inventor
김준기
Original Assignee
재단법인 아산사회복지재단
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Priority claimed from KR1020170126914A external-priority patent/KR101908328B1/ko
Publication of WO2018066940A1 publication Critical patent/WO2018066940A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G02B21/04—Objectives involving mirrors

Definitions

  • Various embodiments of the present invention are implemented to be coupled to an existing optical microscope, and relates to a multiple endoscope coupling module for simultaneously observing a plurality of observation points (ie, biological tissues of different organs) or a plurality of biological samples in a living biological sample will be.
  • a plurality of observation points ie, biological tissues of different organs
  • a plurality of biological samples in a living biological sample will be.
  • Microscopes are widely used to magnify images of samples, but there are some difficulties in observing a living biological sample in various ways.
  • FIG. 1 is a view showing a general optical microscope.
  • the optical microscope 10 is difficult to observe when there is a biological sample to be observed in the transverse direction because the direction of the objective lens 11 is substantially fixed.
  • the space between the objective lens 11 and the object stage 12 is limited, it is difficult to position a plurality of lenses, and thus there is a limit in observing various parts of the biological sample at the same time.
  • Confocal laser microscopy which provides a plurality of laser lights, also provides multiple laser lights for a particular point of observation to confirm the results of reaction to each laser light. That is, there is no method that can identify several biological samples or multiple observation points in one biological sample at the same time using a single microscope.
  • the problem to be solved by the present invention is to provide a multiple endoscope coupling module, which is configured to be coupled to the existing microscope structure, to identify multiple biological samples or multiple observation points in one biological sample at the same time using a single microscope. do.
  • various embodiments of the present invention is to provide a multiple endoscope coupling module that can be easily attached to and detached from a variety of optical microscopes possessed by many biomedical laboratories and tissue laboratory.
  • multiple endoscope coupling module coupled to the optical microscope to observe a plurality of biological samples or a plurality of points in a specific biological sample at the same time through the optical microscope
  • An objective lens fastening part connected to the objective lens of the optical microscope or the objective lens holder from which the objective lens is removed;
  • a light path converting adapter for switching a path of the plurality of laser lights provided from the optical microscope;
  • a light control adapter connected to the light path conversion adapter to split a plurality of laser lights;
  • a plurality of probe mounts coupled to the light control adapter to provide specific laser light to the biological sample, wherein the optical path conversion adapter includes one or more binding adapters, and the probe mount is inserted into the biological sample.
  • the coupling adapter includes a dichroic mirror that reflects or transmits and splits a plurality of laser lights based on a specific reference wavelength. After providing the optical axis, the laser light reflected from the biological sample is divided to generate an image for each laser light.
  • the dichroic mirror may reflect laser light having a wavelength shorter than a specific reference wavelength among the laser lights, and transmit the remaining light.
  • the light control adapter when the light control adapter includes N-1 coupling adapters (N is a natural number of 2 or more), the k-1th coupling adapter (k is larger than 1 and smaller than N-1).
  • the reference wavelength of the dichroic mirror in the same natural number) is shorter than the reference wavelength of the dichroic mirror in the k-th coupling adapter, and the light adjusting adapter separates the N laser lights by reflection or transmission. It is done.
  • the first coupling adapter is coupled to the optical path conversion adapter, and the k-th coupling adapter is used. Is coupled to the k-1 coupling adapter, the first probe mount is coupled to a direction in which the laser light reflected by the dichroic mirror in the first coupling adapter travels, and the k-1 probe mount is And is coupled to a direction in which the laser light reflected by the dichroic mirror in the k-th coupling adapter travels.
  • the light control adapter further comprises an N-th coupling adapter connected to the N-th coupling adapter and including a general mirror, and the probe mount is reflected from the N-th coupling adapter. It further comprises an N-th probe mount coupled to the direction in which the laser light proceeds.
  • the optical path converting adapter may include: a first reflector reflecting a laser light in a vertical direction provided from the objective lens holder to change an optical path in a horizontal direction; And a first lens and a second lens for controlling the reflected laser light.
  • the optical path conversion adapter may further include a second reflector disposed after the second lens and converting the laser light traveling in a horizontal direction in a vertical direction.
  • the coupling adapter is sequentially coupled in the vertical direction to the optical path conversion adapter.
  • the objective lens fastening portion is to be replaced or the aperture is adjusted according to the type of the optical microscope.
  • the endoscope probe is made of a material that can be bent.
  • the probe mount may include an objective lens receiving laser light from the coupling adapter; And a first adjuster configured to perform a translational movement in the x-y axis direction of the endoscope probe to match the optical axis provided from the objective lens with the optical axis of the endoscope probe.
  • the conventional optical microscope separates each laser light reflected from the biological sample to generate a separate image
  • the user does not need to perform a separate operation other than connecting the multiple endoscope coupling module to the existing optical microscope.
  • an optical microscope providing a plurality of laser lights having different wavelengths and generating an image for each laser light after being reflected from a sample, separating and separately detecting each reflected light inside the optical microscope Because it includes a dichroic mirror structure, a user can combine multiple endoscope coupling modules capable of distributing a plurality of laser beams having different wavelengths at each observation point to a single optical microscope simply by combining them with an existing optical microscope. It can be extended to check the observation point of the same time.
  • the multiple endoscope coupling module allows the biological sample to be placed and viewed in a spaced apart area, rather than below or above the narrow optical microscope, by the optical path conversion adapter.
  • 1 is a view showing a general optical microscope.
  • FIG. 2 is a structural diagram of a multiple endoscope coupling module according to an embodiment of the present invention.
  • 3A is an exemplary diagram of a multiple endoscope coupling module according to an embodiment of the present invention.
  • Figure 3b is an exemplary view of combining a prototype of a multiple endoscope coupling module according to an embodiment of the present invention to an existing optical microscope.
  • Figure 4 is an exemplary view showing a coupling relationship between the objective lens fastening portion and the optical path conversion adapter according to an embodiment of the present invention.
  • FIG 5 is an exemplary view of an optical structure inside the optical path converting adapter according to an embodiment of the present invention.
  • FIG. 6 is an exemplary view showing a coupling relationship between a coupling adapter and a probe mount according to an embodiment of the present invention.
  • FIG. 7 and 8 are exemplary diagrams illustrating a coupling relationship between a light path conversion adapter, a plurality of coupling adapters, and a plurality of probe mounts according to an embodiment of the present invention.
  • spatially relative terms below “, “ beneath “, “ lower”, “ above “, “ upper” It can be used to easily describe a component's correlation with other components. Spatially relative terms are to be understood as including terms in different directions of components in use or operation in addition to the directions shown in the figures. For example, when flipping a component shown in the drawing, a component described as “below” or “beneath” of another component may be placed “above” the other component. Can be. Thus, the exemplary term “below” can encompass both an orientation of above and below. Components may be oriented in other directions as well, so spatially relative terms may be interpreted according to orientation.
  • An existing confocal laser microscope provides a plurality of lasers having different wavelength bands inside a microscope to a specific sample, and receives and shows each laser light reflected or transmitted from the sample to the user.
  • Confocal means removing the light out of focus of the sample and using the light that is in focus.
  • the elements that can be observed in a particular sample differ depending on the wavelength of the laser light, and conventional confocal microscopes provide laser light of different wavelength bands so that various elements in the sample can be identified at a time.
  • conventional confocal microscopy uses dichroic mirrors to separate a plurality of lasers transmitted or reflected through a sample.
  • a plurality of laser beams can be combined to provide a single sample, and a plurality of laser beams reflected or transmitted from the sample can be divided into confocal laser microscopes that can be generated as separate images. You need a module that can do that.
  • the optical microscope 10 may be various microscopes such as a confocal microscope, a multiphoton microscope, a brightfield / darkfield microscope, and the like, which are held by a laboratory, a histology laboratory, and the like.
  • the multiple endoscope coupling module 20 is used in combination with the inverted microscope is described as an example.
  • the multiple endoscope coupling module 20 according to the present embodiment may be used in combination with other microscopes such as an upright microscope. Of course.
  • FIG. 2 is a structural diagram of a multiple endoscope coupling module 20 according to an embodiment of the present invention.
  • FIG 3 is a view illustrating a state in which the multiple endoscope coupling module 20 is coupled to the inverted optical microscope 10 according to an embodiment of the present invention.
  • the multiple endoscope coupling module according to an embodiment of the present invention, the objective lens fastening portion 100; Light path conversion adapter 200; Light control adapter 300 and probe mount 400.
  • the objective lens fastening part 100 may be coupled to the objective lens part of the silver optical microscope 10.
  • the objective coupling part 100 is directly connected to the optical microscope 10 to correspond to the objective lens or connected to the objective lens holder in a state in which the objective lens is removed.
  • the objective lens coupling unit 100 may be equipped with an objective lens 110 serving as an objective lens of the optical microscope 10.
  • the objective lens coupling part 100 coupled to the optical microscope 10 is configured to be compatible with various optical microscopes 10.
  • the objective fastener 100 may be implemented in consideration of the objective holder holder (internal thread) of several microscope suppliers.
  • the fastening part may have a structure in which the object lens holder of the optical microscope 10 is fastened or rotated in the form of a female screw or a male screw. That is, as shown in Figure 4, the objective lens fastening portion 100 may be connected to the optical path conversion adapter 200 is replaced according to the type of optical microscope used.
  • the objective lens coupling portion 100 of the multiple endoscope coupling module 20 may be adjustable in size.
  • the objective lens coupling unit 100 may be adjusted in the size of the insertion hole is inserted into the objective lens holder in accordance with the operation by the user (for example, by screwing).
  • the optical path conversion adapter 200 serves to change the path of the laser light provided from the objective lens unit of the optical microscope 10. That is, the optical path converting adapter 200 converts the optical paths so that the sample may be placed and observed in a space spaced apart from the optical microscope 10 without being limited to a narrow space below or above the optical microscope 10. give. Specifically, the optical path converting adapter 200 changes the path of the upright observation light into the horizontal observation light, so that the observation image in the vertical or horizontal direction inside the sample acquired through the probe mount 400. Can be observed through the optical microscope (10).
  • the light path conversion adapter 200 may be divided into a light reflecting unit 210 and a light refracting unit 220, as shown in FIG.
  • the objective lens fastening part 100 is connected to the light reflection part 210 and coupled to the optical microscope, and the light refraction part 220 may be fitted to one side of the light reflection part 210. Through this, the user can easily combine the optical path conversion adapter 200 to the optical microscope 10.
  • the light path conversion adapter 200 may be designed to receive related optical elements, as in FIG. 5.
  • the optical path converting adapter 200 may include a reflector 211 for changing the optical path by reflecting light exiting from the objective lens holder of the optical microscope 10, a first lens 221 for controlling the reflected light, and It may include a second lens 222.
  • the light path conversion adapter 200 is for flipping a conjugate scanning plane, and according to an embodiment, may be formed of a 4f lens system.
  • the first lens 221 is spaced one focal length f from the scanning plane, and the second lens 222 is spaced two focal lengths 2f from the first lens 221.
  • the objective lens in the probe mount 400 may be spaced apart from the second lens 222 by one focal length f.
  • the light control adapter 300 serves to provide the light provided from the light path conversion adapter 200 to the probe mount 400 to be described later through reflection or transmission.
  • the light conditioning adapter 300 includes one or more coupling adapters 310.
  • the coupling adapter may be manufactured in the form of a module to be coupled to and separated from the light control adapter 300.
  • the coupling adapters 310 and 320 include dichroic mirrors 311 and 321 to transmit laser light of a specific reference wavelength or more and to reflect laser light having a wavelength shorter than the specific reference wavelength. do.
  • a dichroic mirror is a reflector made of a thin layer of a material having a different refractive index. The dichroic mirror reflects light of a certain color (for example, a light source) and transmits light of a different color.
  • a plurality of dichroic mirrors used in embodiments of the present invention are used in different kinds to transmit or reflect light sources of different wavelengths or colors.
  • the laser light shorter than the reference wavelength among the laser light provided by the optical path conversion adapter (for example, provided from an optical microscope)
  • the laser light includes red light, green light and blue light, and the reference wavelength is between the green light wavelength and the blue light wavelength, blue light
  • the coupling adapter 310 may connect the probe mount to the reflected blue light path and the transmitted red light and green light path.
  • the coupling adapter 310 is connected to an optical microscope that provides a plurality of lasers having different wavelengths with the same optical axis, and divides the laser based on a specific wavelength to identify the plurality of points.
  • the first coupling adapter 310 is connected to the optical path conversion adapter 200.
  • the second coupling adapter is coupled to the transmitted light propagation path of the first coupling adapter 310.
  • the reference wavelength of the dichroic mirror 311 of the first coupling adapter 310 is between the blue light wavelength and the green light wavelength
  • the reference wavelength of the dichroic mirror 321 of the second coupling adapter 320 is the green light wavelength and the red light.
  • the first coupling adapter 310 reflects the blue light and provides it to the first probe mount 410, and the second coupling adapter 320 passes through the first coupling adapter 310 and the red light.
  • Green light is reflected to the second probe mount 320.
  • the red light passing through the first coupling adapter and the second coupling adapter is provided to the third probe mount 330.
  • a third coupling adapter in which a general mirror other than a dichroic mirror is installed may be connected to the second coupling adapter 320 to reflect red light to provide the third probe mount.
  • the optical path conversion adapter 200 After the optical path conversion adapter 200 reflects the laser light incident in the vertical direction through the objective lens coupling part 100 from the second reflection surface, the optical path conversion adapter 200 converts the laser light into the horizontal direction. The optical path can be converted again in the vertical direction on the second reflecting surface.
  • the plurality of coupling adapters 310 and 320 included in the light control adapter 300 may be coupled to the light path conversion adapter 200 in order.
  • the laser light includes blue light, green light, and red light
  • a first coupling adapter 310 reflecting only blue light is connected to the optical path conversion adapter 200 to reflect the blue light to provide to the first probe mount 410. It transmits green light and red light.
  • the second coupling adapter 320 is connected to the first coupling adapter 310 to reflect the green light to the second probe mount 420, and transmits the red light.
  • a base adapter 330 having a general mirror 331 instead of a dichroic mirror is connected to the second coupling adapter 320 and reflects red light to provide to the third probe mount 430.
  • the plurality of coupling adapters included in the light control adapter 300 may be continuously coupled to the light path conversion adapter 200. That is, when N (N is a natural number greater than 2) light is combined and provided by the optical microscope 10, N-1 (for example, without using a separate coupling adapter for the N-th light) When provided to the probe mount in the transmitted state, or N (for example, when a coupling adapter equipped with a general mirror reflecting the Nth light is connected), the coupling adapter is sequentially connected to the optical path conversion adapter 200. Can be connected.
  • the first coupling adapter 310 is connected to the optical path conversion adapter 200
  • the second coupling adapter 320 is connected to the opposite side of the position of the first coupling adapter 310 connected to the optical path conversion adapter 200
  • the third coupling adapter 330 is connected to the opposite side of the position of the second coupling adapter 320 to which the first coupling adapter 310 is connected.
  • the coupling adapters 310 and 320 may open at least a portion of the module surface to receive light reflected from the dichroic mirrors 311 and 321 in each module. It can be prepared as. Accordingly, the user may check the desired number of images by combining the coupling adapter with another coupling adapter according to the number of images to be additionally checked.
  • one or more coupling adapters may be referred to as indicating respective areas where the inner region of the light conditioning adapter 300 or the light path conversion adapter 200 is arbitrarily partitioned.
  • the first coupling adapter is included in the optical path conversion adapter 200, and only the coupling adapter additionally connected may be manufactured in a separate module form.
  • the laser light separated from one imaging system reaches the biological sample through three objective lenses, so that the user may simultaneously check three different fluorescent images on the biological sample.
  • each coupling adapter (310, 320, 330, etc.) in the light control adapter 300 may be manufactured to be rotatable after being connected to the optical path conversion adapter 200 or other coupling adapter. That is, the coupling adapter may move the transmitted laser light along the same optical path, and change the direction in which the reflected laser light is provided. For example, when the first coupling adapter 310 transmits the red light and the green light in the laser light combined with the red light, the green light, and the blue light, and the dichroic mirror 311 reflects the blue light, the first coupling adapter may be It can be switched from the arrangement to reflect the blue light in the direction to the arrangement to reflect to the side by rotation. At this time, as the first coupling adapter 310 rotates, the direction of the first probe mount connected to the first coupling adapter is also changed and disposed in the lateral direction.
  • the probe mount 400 is coupled to one side of the light control adapter 300 to provide a laser light separated from the sample. That is, the probe mount 400 is connected to each coupling adapter.
  • the probe mount 400 may include an objective lens for providing a laser beam provided from the light control adapter 300 to the probe.
  • probe mounts may be included in place of objective lenses placed in an optical microscope.
  • the probe mount 400 includes an endoscope probe 401 at least partially inserted into the biological sample.
  • One end of the endoscope probe 401 is positioned in contact with the portion of the biological sample to be observed.
  • the laser light provided from the light source of the optical microscope 10 passes through the light path conversion adapter 200, the light control adapter 300, and the endoscope probe 401 to the biological sample, and the reflected light from the biological sample is transmitted to the endoscope probe ( 401, the light control adapter 300, and the light path conversion adapter 200 are transmitted to the inside of the optical microscope 10 so that the user can observe the biological sample through the optical microscope 10.
  • the optical microscope 10 may generate a separate image by dividing the laser light reflected at each position after being mixed and received again. That is, the optical microscope 10 divides the laser light reflected from a plurality of points of the biological sample by the dichroic mirror provided therein to produce an individual image.
  • the probe mount 400 may be connected to various types of probes.
  • the probe mount may include endoscope probes p1, p2, p3 having flexible connections, as probes.
  • the endoscope probe is aligned with the same optical axis as the laser light reflected or transmitted from the coupling adapter by the probe mount, so that the laser light is incident.
  • a particular probe mount may comprise a rigid probe.
  • each probe mount 400 is equipped with a rigid probe to each biosample. It can be inserted in.
  • the endoscope probe 401 may be a GRIN probe including a gradient index lens.
  • the endoscope probe 401 may be composed of a single lens or a plurality of lenses, and its length, diameter, and material (eg, hard probe, soft probe, etc.) may vary according to a biological sample to be observed.
  • the probe mount 400 of the multiple endoscope coupling module 20 is inserted into at least a portion of the biological sample endoscope probe 401, the endoscope at one end of the endoscope probe 401 for observing the biological sample Of the biological sample obtained through the holder 431 holding the probe 401, the first adjusting unit 432 for enabling the xy-axis translational movement of the endoscope probe 401, and the endoscope probe 401.
  • In-situ rotational movement of the objective lens 433, the second adjusting unit (Obj. Lens Z stage, 434) to enable the translation of the z-axis of the objective lens 433 to enlarge the image of the image, and the endoscope probe 401. It may include all or part of the third adjusting portion (R stage knob, 435) to enable.
  • the objective lens 433 and the endoscope probe 401 in the probe mount 400 should be aligned with the same optical axis, so that the laser beam passing through the objective lens 433 may be provided into the endoscope probe 401.
  • the probe mount 400 includes the first adjusting unit 432
  • the endoscope probe 401 is moved in the two-dimensional direction (xy plane) by the first adjusting unit 432, so that the laser light It is matched with the direction provided by the objective lens.
  • the third control unit 435 may include a fine rotation stage.
  • the in-situ rotational motion of the endoscope probe 401 may be controlled by the rotation of the microrotation stage, and when the microrotation stage rotates, it may be transmitted to the endoscope probe 401 through a holder 431 connected to one end of the microrotation stage. have.
  • multiple endoscope coupling modules 20 in accordance with embodiments of the present invention, researchers have previously observed several observation points (eg, different points or different points of a living biological sample) that could not be simultaneously identified with one optical microscope. Physiological changes occurring in the individual) can be monitored simultaneously.
  • the researchers can connect endoscopes to multiple points in a living biological sample to compare the amount and rate of drug delivery to the body. That is, the user can compare and analyze the pharmacokinetics / pharmacodynamics of the drug. For example, you can determine which liver or kidney arrives first, which cells respond first, and so on.
  • the researcher can observe the activation state of the brain cells at the same time while watching the response of the immune cells present in the intestine. That is, the researcher can grasp the relationship between intestinal immune cells and brain cell activation in response to external stimuli in one individual by using the multiple endoscope coupling module 20 according to the embodiments of the present invention in one optical endoscope. .
  • the multiple endoscope coupling module 20 can be used by connecting to the optical microscope 10 are not limited thereto, and one optical fiber providing a plurality of laser lights having different wavelengths.
  • the microscope 10 can be used in various situations where multiple observation points must be identified simultaneously.
  • the conventional optical microscope separates each laser light reflected from the biological sample to generate a separate image
  • the user does not need to perform a separate operation other than connecting the multiple endoscope coupling module to the existing optical microscope.
  • an optical microscope providing a plurality of laser lights having different wavelengths and generating an image for each laser light after being reflected from a sample, separating and separately detecting each reflected light inside the optical microscope Because it includes a dichroic mirror structure, a user can combine multiple endoscope coupling modules capable of distributing a plurality of laser beams having different wavelengths at each observation point to a single optical microscope simply by combining them with an existing optical microscope. It can be extended to check the observation point of the same time.
  • the multiple endoscope coupling module allows the biological sample to be placed and viewed in a spaced apart area, rather than below or above the narrow optical microscope, by the optical path conversion adapter.

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Abstract

La présente invention concerne un module de couplage d'endoscope multiplex. Un module de couplage d'endoscope multiplex selon un mode de réalisation de la présente invention comprend : une partie de fixation de lentille d'objectif reliée à une lentille d'objectif d'un microscope optique ou à un support de lentille d'objectif dont la lentille d'objectif a été retirée ; un adaptateur de conversion de trajet optique pour la commutation du trajet d'une pluralité de lumières laser en provenance du microscope optique ; un adaptateur de réglage optique relié à l'adaptateur de conversion de trajet optique pour diviser une pluralité de lumières laser ; et une pluralité de supports de sonde reliés à l'adaptateur de réglage optique pour la fourniture d'une lumière laser particulière à un échantillon biologique. L'adaptateur de conversion de trajet optique comprend un ou plusieurs adaptateurs de couplage, le support de sonde comprend une sonde d'endoscope qui doit être insérée dans l'échantillon biologique, l'adaptateur de couplage comprend un miroir dichroïque pour réfléchir ou transmettre une pluralité de lumières laser sur la base d'une longueur d'onde de référence prédéterminée, afin de diviser la pluralité de lumières laser, et le microscope optique fournit une pluralité de lumières laser le long du même axe optique, puis divise les lumières laser fournies réfléchies par un échantillon biologique, et génère une image de chacune des lumières laser.
PCT/KR2017/011004 2016-10-06 2017-09-29 Module de couplage d'endoscope multiplex WO2018066940A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20160128849 2016-10-06
KR10-2016-0128849 2016-10-06
KR10-2017-0126914 2017-09-29
KR1020170126914A KR101908328B1 (ko) 2016-10-06 2017-09-29 다중 내시경 결합 모듈

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04409A (ja) * 1989-09-22 1992-01-06 Fuji Photo Film Co Ltd 共焦点走査型顕微鏡
JP2002139675A (ja) * 2000-10-31 2002-05-17 Olympus Optical Co Ltd レーザ顕微鏡
US20080062401A1 (en) * 2004-07-02 2008-03-13 Koninklijke Philips Electronics N.V. Spectroscopic System With Multiple Probes
KR101356708B1 (ko) * 2012-07-05 2014-02-05 한국과학기술원 무흉터 내시경 수술용 멀티모달 공초점 내시 현미경
JP2016080494A (ja) * 2014-10-16 2016-05-16 日本分光株式会社 近接場測定方法および近接場光学顕微鏡

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04409A (ja) * 1989-09-22 1992-01-06 Fuji Photo Film Co Ltd 共焦点走査型顕微鏡
JP2002139675A (ja) * 2000-10-31 2002-05-17 Olympus Optical Co Ltd レーザ顕微鏡
US20080062401A1 (en) * 2004-07-02 2008-03-13 Koninklijke Philips Electronics N.V. Spectroscopic System With Multiple Probes
KR101356708B1 (ko) * 2012-07-05 2014-02-05 한국과학기술원 무흉터 내시경 수술용 멀티모달 공초점 내시 현미경
JP2016080494A (ja) * 2014-10-16 2016-05-16 日本分光株式会社 近接場測定方法および近接場光学顕微鏡

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