WO2018021717A1 - Module de couplage endomicroscopique - Google Patents

Module de couplage endomicroscopique Download PDF

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Publication number
WO2018021717A1
WO2018021717A1 PCT/KR2017/007209 KR2017007209W WO2018021717A1 WO 2018021717 A1 WO2018021717 A1 WO 2018021717A1 KR 2017007209 W KR2017007209 W KR 2017007209W WO 2018021717 A1 WO2018021717 A1 WO 2018021717A1
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WO
WIPO (PCT)
Prior art keywords
endoscope
microscope
probe
coupling module
biological sample
Prior art date
Application number
PCT/KR2017/007209
Other languages
English (en)
Korean (ko)
Inventor
김준기
Original Assignee
재단법인 아산사회복지재단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 재단법인 아산사회복지재단 filed Critical 재단법인 아산사회복지재단
Publication of WO2018021717A1 publication Critical patent/WO2018021717A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00163—Optical arrangements
    • A61B1/00188—Optical arrangements with focusing or zooming features
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems
    • G02B26/103—Scanning systems having movable or deformable optical fibres, light guides or waveguides as scanning elements

Definitions

  • the present invention relates to an endoscope microscope coupling module, and more particularly, to an endoscope microscope coupling module that is coupled to a microscope to observe a biological sample through a microscope.
  • Microscopes are widely used to magnify images of samples, but there are some difficulties in observing a living biological sample in various ways.
  • 1 is a view showing a general optical microscope.
  • the optical microscope 10 is difficult to observe when there is a biological sample to be observed in the transverse direction because the direction of the objective lens 11 is substantially fixed.
  • the space between the objective lens 11 and the object stage 12 is limited, there is a limit in observing various parts of the biological sample.
  • the present invention seeks to provide an endoscope microscope coupling module that can be easily attached to and detached from various types of optical microscopes possessed by many biomedical laboratories and histological laboratories.
  • the present invention is to provide an endoscope microscope coupling module that can observe the inside of a living biological sample in the transverse and longitudinal direction.
  • An endoscope microscope coupling module coupled to a microscope to observe a biological sample through a microscope according to an embodiment of the present invention
  • the optical path conversion adapter coupled to the objective lens of the microscope and changes the path of the observation light
  • a probe mount located on one side of the optical path conversion adapter, the probe mount including an endoscope probe inserted into at least a part of the biological sample, wherein the optical path conversion adapter rotates the probe mount to the ground;
  • an adjusting unit for adjusting the angle of the endoscope probe is
  • the adjustment unit includes a switching knob rotatably configured to rotate the endoscope probe with respect to the ground, and a reflector reflecting light incident through the light path conversion adapter.
  • the reflector is fixed to the switching knob, the angle is changed according to the rotation of the switching knob.
  • the reflector reflects the incident light toward the endoscope probe as the switching knob rotates.
  • the reflector has a structure that rotates or folds according to the rotation of the switching knob.
  • the angle is adjusted from 0 degrees to 90 degrees with respect to the ground in accordance with the rotation of the probe mount.
  • the probe mount may include a first adjuster for adjusting the xy-axis translational movement of the endoscope probe and a second adjuster for adjusting the rotational movement of the endoscope probe in the longitudinal direction.
  • an endoscope microscope coupling module capable of observing the inside of a living biological sample in the transverse and longitudinal directions can be provided.
  • 1 is a view showing a general optical microscope.
  • FIG. 2 is a view for explaining the endoscope microscope coupling module according to an embodiment of the present invention.
  • Figure 3 is a perspective view of the endoscope microscope coupling module from another angle.
  • FIG. 4 is a view for explaining an example of observing a biological sample by using an endoscope microscope coupling module according to an embodiment of the present invention.
  • FIG. 5 is a view for explaining a path change of light output to an endoscope probe through a reflector located inside the adjustment unit of FIG. 3.
  • FIG. 6 is an enlarged view of an example of a structure of part A of the endoscope microscope coupling module in FIG. 2.
  • FIG. 2 is a view for explaining the endoscope microscope coupling module according to an embodiment of the present invention.
  • FIG. 2 (a) is a view showing a state in which the endoscope microscope coupling module 100 is coupled to a general inverted optical microscope 10
  • Figure 2 (b) is a longitudinal side view of the endoscope microscope coupling module 100
  • 2 (c) is a diagram showing the internal configuration of the endoscope microscope coupling module 100.
  • the endoscope microscope coupling module 100 may be coupled to the objective lens unit of the optical microscope 10.
  • the endoscope microscope coupling module 100 may include a fastening part connected to the objective lens holder of the optical microscope 10.
  • the optical microscope 10 may be various microscopes such as a confocal microscope, a multiphoton microscope, a brightfield / darkfield microscope, and the like, which are held by a laboratory, a histology laboratory, and the like.
  • the case where the endoscope microscope coupling module 100 is used in combination with the inverted microscope is described as an example, but the endoscope microscope coupling module 100 according to the present embodiment may be used in the upright microscope.
  • the fastening portion coupled to the optical microscope 10 is configured to be compatible with various optical microscopes 10.
  • the fastening portion coupled to the optical microscope 10 in the endoscope microscope coupling module 100 may be implemented in consideration of objective lens holder specifications of various microscope suppliers.
  • the fastening part may have a structure in which the objective lens holder of the optical microscope 10 is fastened in the form of a female screw / male thread.
  • the endoscope microscope coupling module 100 may be coupled to the optical microscope 10 so as to correspond to the objective lens of the optical microscope 10, the objective of the objective lens is removed from the optical microscope 10 It may also be coupled to the lens holder.
  • the endoscope microscope coupling module 100 is coupled to the optical microscope 10 to one side of the optical path conversion adapter 200 and the optical path conversion adapter 200 to change the path of the observation light. It includes a probe mount (300) located.
  • the optical path converting adapter 200 is coupled to the objective lens unit of the optical microscope 10 and serves to change the path of the observation light. Specifically, the optical path conversion adapter 200 changes the path of the upright observation light into the horizontal observation light, so that the observation image in the vertical or horizontal direction inside the sample obtained through the probe mount 300. Can be observed through the optical microscope (10).
  • the probe mount 300 includes an endoscope probe 301 at least partially inserted into the biological sample.
  • One end of the endoscope probe 301 is positioned in contact with the portion of the biological sample to be observed.
  • Light from the light source of the optical microscope 10 passes through the optical path converting adapter 200 and the endoscope probe 301 to the biological sample, and the reflected light from the biological sample is transmitted to the endoscope probe 301 and the optical path converting adapter ( Passing through the optical microscope 10 to the eyepiece of the optical microscope 10, the user can observe the biological sample through the optical microscope (10).
  • the endoscope probe 301 may be a GRIN probe consisting of a gradient index lens.
  • the endoscope probe 301 may be formed of a single lens or a plurality of lenses, and its length, diameter, and material (eg, hard probe, soft probe, etc.) may vary according to a biological sample to be observed.
  • the light path conversion adapter 200 may be designed to accommodate related optical elements.
  • the optical path conversion adapter 200 reflects the light exiting from the objective lens mount 210 and the objective lens mount 210 coupled to the objective lens unit of the optical microscope 10 to change the optical path ( 210, the first lens 220, the second lens 230, and the objective lens 250 for controlling the reflected light may be included.
  • the objective lens mount 210 may be equipped with an objective lens 211 serving as an objective lens of the optical microscope 10.
  • the light path conversion adapter 200 is for flipping a conjugate scanning plane, and according to an embodiment, may be formed of a 4f lens system.
  • the first lens 230 is spaced one focal length f from the scanning plane
  • the second lens 240 is spaced two focal lengths 2f from the first lens 230
  • the The back aperture may be spaced one focal length f from the second lens 240.
  • Figure 3 is a perspective view of the endoscope microscope coupling module from another angle.
  • the endoscope microscope coupling module 100 includes an adjustment unit 400 for rotating the probe mount 300 to adjust an angle of the endoscope probe 301 with respect to the ground.
  • the adjustment unit 400 may be configured such that the angle of the probe mount 300 (eg, 0 degrees or more and 90 degrees or less) so that the endoscope probe 301 makes an angle perpendicular to the ground, or vertically or horizontally. Angle) can be adjusted.
  • the adjusting unit 400 may include a reflector (not shown) reflecting light incident through the switching knob 410 and the light path conversion adapter 200 rotatably configured to rotate the endoscope probe 301 with respect to the ground. Include. The process of reflecting the incident light through the reflector of the adjustment unit 400 will be described in more detail with reference to FIG. 5.
  • the user of the optical microscope 10 is coupled to the endoscope microscope coupling module 100 to the optical microscope 10, the probe mount 300 and the endoscope by rotating the switching knob 410 according to the type and observation position of the biological sample
  • the position of the probe 301 and the angle with respect to the ground may be adjusted.
  • FIG. 4 is a view for explaining an example of observing a biological sample by using an endoscope microscope coupling module according to an embodiment of the present invention.
  • the endoscope probe 301 is positioned perpendicular to the ground when the internal organs are to be observed after the opening of the biological sample.
  • the adjustment unit 400 may be adjusted to be used, and as shown in FIG. 4B, when the inner wall, tubular organs, respiratory organs, etc. of the internal organs of the biological sample are to be observed, the endoscope probe 301 may be grounded.
  • the adjustment unit 400 can be adjusted to be in parallel with the.
  • the probe mount 300 may rotate the first adjuster 310 and the endoscope probe 301 in the longitudinal direction to control the xy-axis translational movement of the endoscope probe 301. It may include a second adjusting unit 320 to adjust.
  • the rotational movement with respect to the longitudinal axis of the endoscope probe 301 means the in-situ rotation about the axis of the longitudinal direction, not the rotation of the endoscope probe 301 with respect to the ground.
  • the user of the optical microscope 10 may adjust the position and direction of the endoscope probe 301 using the first adjusting unit 310 and the second adjusting unit 320 according to the type and observation position of the biological sample. .
  • FIG. 5 is a view for explaining a path change of light output to an endoscope probe through a reflector located inside the adjustment unit of FIG. 3.
  • the adjustment unit 400 is located at one side of the light path conversion adapter 200 and includes a reflector 420 therein.
  • the adjustment unit 400 may reflect the light incident through the light path conversion adapter 200 toward the endoscope probe 301 of the probe mount 300.
  • the reflector 420 is fixed to the switching knob 410, the angle (angle, position, etc.) is changed according to the operation (rotation) of the switching knob 410, thereby changing the reflection angle of the incident light have. That is, as described above, the user of the optical microscope 10, by rotating the switching knob 410 in accordance with the type and observation position of the sample to the position and the ground of the probe mount 300 and the endoscope probe 301 You can adjust the angle. And the reflector 420, the angle is adjusted together in accordance with the operation of the switching knob 410, by the operation of the switching knob 410, the incident light toward the endoscope probe 301 whose angle to the ground is adjusted By reflecting, light may be supplied to the endoscope probe 301.
  • the reflector 420 serves to allow the user of the optical microscope 10 to observe the biological sample by reflecting the reflected light from the biological sample as described above and supplying it to the optical path conversion adapter 200.
  • the reflector 420 may be a structure that rotates and / or folds up and down according to the rotation of the switching knob 410. Referring to FIGS. 5C and 5D, it can be seen that the path of the light reflected by the reflector 420 changes as the reflector 420 rotates and / or folds.
  • FIG. 6 is an enlarged view of an example of a structure of part A of the endoscope microscope coupling module in FIG. 2.
  • the endoscope microscope coupling module 100 is inserted into at least a portion of the biological sample endoscope probe 301 for observing the biological sample, the endoscope probe 301 at one end of the endoscope probe 301
  • the image of the image of the biological sample obtained through the holder 331, the first control unit (Probe xy stage, 332) to enable the xy-axis translational movement of the endoscope probe 301, the endoscope probe 301
  • Lens Z stage, 334) for enabling the z-axis translational movement of the objective lens 333, and enabling the in-situ rotational movement of the endoscope probe 301
  • the third adjusting unit (R stage knob, 335) may be included, and at least some of the above components may constitute the probe mount 300.
  • the third adjusting unit 335 may include a fine rotation stage 336.
  • the in-situ rotational motion of the endoscope probe 301 may be controlled by the rotation of the microrotation stage 336, and when the microrotation stage 336 rotates, the holder 331 is connected to one end of the microrotation stage 336. It may be delivered to the endoscope probe 301.
  • an endoscopic microscope coupling module capable of observing a biological sample in the transverse and longitudinal directions can be provided.

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  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Surgery (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Veterinary Medicine (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Ophthalmology & Optometry (AREA)
  • Microscoopes, Condenser (AREA)
  • Endoscopes (AREA)

Abstract

La présente invention concerne un module de couplage endomicroscopique accouplé à un microscope de sorte qu'un échantillon biologique puisse être observé à travers le microscope. Selon un mode de réalisation de la présente invention, le module de couplage endomicroscopique comprend : un adaptateur de changement de trajet optique accouplé à une unité de lentille d'objectif du microscope et qui change le trajet de la lumière d'observation ; et un support de sonde positionné d'un côté de l'adaptateur de changement de trajet optique et comprenant une sonde endoscopique dont au moins une partie est introduite dans un échantillon biologique. L'adaptateur de changement de trajet optique comprend une unité de réglage permettant de régler l'angle de la sonde endoscopique par rapport à la surface du sol par rotation du support de sonde. La présente invention porte également sur un module de couplage endomicroscopique, qui peut être facilement fixé à/détaché de divers types de microscopes optiques dont disposent de nombreux laboratoires biomédicaux et laboratoires de biopsie.
PCT/KR2017/007209 2016-07-25 2017-07-06 Module de couplage endomicroscopique WO2018021717A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0093874 2016-07-25
KR1020160093874A KR101814061B1 (ko) 2016-07-25 2016-07-25 내시경 현미경 결합 모듈

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WO2018021717A1 true WO2018021717A1 (fr) 2018-02-01

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101908328B1 (ko) 2016-10-06 2018-12-18 재단법인 아산사회복지재단 다중 내시경 결합 모듈
KR102258893B1 (ko) * 2019-11-28 2021-05-31 재단법인대구경북과학기술원 내시현미경의 수직 분해능 최적화 장치, 최적화 광학 시스템 및 최적화 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0759723A (ja) * 1993-07-30 1995-03-07 Carl Zeiss:Fa 内視鏡を先に接続した観察及び／又は文書化手段並びにそれを動作させる方法
JP2005134537A (ja) * 2003-10-29 2005-05-26 Olympus Corp 立体視観察装置
JP2006006417A (ja) * 2004-06-22 2006-01-12 Mitaka Koki Co Ltd 手術顕微鏡用の側視型延長鏡筒
JP2009074886A (ja) * 2007-09-20 2009-04-09 Nikon Corp イメージガイド
US20090103174A1 (en) * 2007-03-29 2009-04-23 Junichi Nozawa Surgical observation system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0759723A (ja) * 1993-07-30 1995-03-07 Carl Zeiss:Fa 内視鏡を先に接続した観察及び／又は文書化手段並びにそれを動作させる方法
JP2005134537A (ja) * 2003-10-29 2005-05-26 Olympus Corp 立体視観察装置
JP2006006417A (ja) * 2004-06-22 2006-01-12 Mitaka Koki Co Ltd 手術顕微鏡用の側視型延長鏡筒
US20090103174A1 (en) * 2007-03-29 2009-04-23 Junichi Nozawa Surgical observation system
JP2009074886A (ja) * 2007-09-20 2009-04-09 Nikon Corp イメージガイド

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