WO2018020435A1 - Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces - Google Patents

Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces Download PDF

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Publication number
WO2018020435A1
WO2018020435A1 PCT/IB2017/054535 IB2017054535W WO2018020435A1 WO 2018020435 A1 WO2018020435 A1 WO 2018020435A1 IB 2017054535 W IB2017054535 W IB 2017054535W WO 2018020435 A1 WO2018020435 A1 WO 2018020435A1
Authority
WO
WIPO (PCT)
Prior art keywords
tissue
thermolysin
bacterial biofilm
biofilm
ulcer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2017/054535
Other languages
English (en)
French (fr)
Inventor
Lei Shi
Catherine VAN DER KAR
Aleksa Jovanovic
Eric Roche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Smith and Nephew Inc
Original Assignee
Smith and Nephew Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smith and Nephew Inc filed Critical Smith and Nephew Inc
Priority to JP2019503673A priority Critical patent/JP7127013B2/ja
Priority to CN201780046178.1A priority patent/CN109475609A/zh
Priority to CA3031866A priority patent/CA3031866A1/en
Priority to EP17758624.5A priority patent/EP3490588A1/en
Priority to AU2017304271A priority patent/AU2017304271B2/en
Priority to US16/319,927 priority patent/US11628207B2/en
Publication of WO2018020435A1 publication Critical patent/WO2018020435A1/en
Anticipated expiration legal-status Critical
Priority to JP2022089557A priority patent/JP2022111186A/ja
Priority to US18/120,726 priority patent/US20230346898A1/en
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18—Nitro compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
    • A61L29/08—Materials for coatings
    • A61L29/085—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
    • A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
    • A61L29/16—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24027—Thermolysin (3.4.24.27)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A61L2300/254—Enzymes, proenzymes

Definitions

  • Embodiment 15 is the method of any one of embodiments 2 to 14, wherein the carrier is a lotion, solution, suspension, liquid, emulsion, cream, gel, ointment, paste, aerosol spray, aerosol foam, non-aerosol spray, non-aerosol foam, film, or sheet.
  • Embodiment 16 is the method of embodiment 15, wherein the carrier is a pharmaceutically acceptable carrier.
  • Embodiment 17 is the method of any one of embodiments 1 to 16, wherein the composition further comprises an antibacterial agent.
  • Embodiment 18 is a method of reducing or eliminating a bacterial biofilm on a biological surface, the method comprising administering to the biological surface a composition comprising thermolysin.
  • compositions and methods for their use can "comprise,” “consist essentially of,” or “consist of any of the ingredients or steps disclosed throughout the specification. [00025] It is contemplated that any embodiment discussed in this specification can be implemented with respect to any method or composition of the invention, and vice versa. Furthermore, compositions of the invention can be used to achieve methods of the invention.
  • the carriers disclosed in US publication 2016/0008293, herein incorporated by reference which are dissolvable gel-forming film compositions with a water content of less than 15% w/w comprising a water-soluble cellulose ether, a hydrophilic rheological modifying agent, and a proteolytic enzyme, wherein the gel-forming film is capable of forming a hydrogel when in contact with water or other aqueous medium, are suitable carriers for topical treatment of skin, mucous membranes, and wounds.
  • Disclosed are methods of treating a wound, mucous membrane lesion, skin lesion, or other biological surface infected or contaminated with a bacterial biofilm the method comprising administering to the wound, mucous membrane lesion, skin lesion, or biological surface a composition comprising thermolysin, wherein the bacterial biofilm is reduced or eliminated.
  • other compositions comprising pharmaceutically active ingredients, cosmetically active ingredients, vulnerary agents, wound healing agents, antibiotics, anti-fungal agents, antiseptic agents, cleansing agents, and/or antibacterial agents, can be administered to the wound, mucous membrane lesion, skin lesion, or biological surface for further treatment.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dermatology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Surgery (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
PCT/IB2017/054535 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces Ceased WO2018020435A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2019503673A JP7127013B2 (ja) 2016-07-27 2017-07-26 表面から細菌バイオフィルムを低減または除去するためのサーモリシンの使用
CN201780046178.1A CN109475609A (zh) 2016-07-27 2017-07-26 使用嗜热菌蛋白酶减少或消除来自表面的细菌生物膜
CA3031866A CA3031866A1 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
EP17758624.5A EP3490588A1 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
AU2017304271A AU2017304271B2 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
US16/319,927 US11628207B2 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
JP2022089557A JP2022111186A (ja) 2016-07-27 2022-06-01 表面から細菌バイオフィルムを低減または除去するためのサーモリシンの使用
US18/120,726 US20230346898A1 (en) 2016-07-27 2023-03-13 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662367338P 2016-07-27 2016-07-27
US62/367,338 2016-07-27

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US16/319,927 A-371-Of-International US11628207B2 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
US18/120,726 Continuation US20230346898A1 (en) 2016-07-27 2023-03-13 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces

Publications (1)

Publication Number Publication Date
WO2018020435A1 true WO2018020435A1 (en) 2018-02-01

Family

ID=59738382

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2017/054535 Ceased WO2018020435A1 (en) 2016-07-27 2017-07-26 Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces

Country Status (7)

Country Link
US (2) US11628207B2 (enExample)
EP (1) EP3490588A1 (enExample)
JP (2) JP7127013B2 (enExample)
CN (1) CN109475609A (enExample)
AU (1) AU2017304271B2 (enExample)
CA (1) CA3031866A1 (enExample)
WO (1) WO2018020435A1 (enExample)

Cited By (1)

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WO2020247582A1 (en) * 2019-06-06 2020-12-10 Danisco Us Inc Methods and compositions for cleaning

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Publication number Priority date Publication date Assignee Title
AU2017304271B2 (en) * 2016-07-27 2024-07-18 Smith & Nephew, Inc. Use of thermolysin to reduce or eliminate bacterial biofilms from surfaces
WO2018140707A1 (en) 2017-01-30 2018-08-02 Smith & Nephew, Inc. Synergistic combination of thermolysin and an antibacterial agent to reduce eliminate bacterial biofilms from surfaces
PH12023550203A1 (en) * 2020-07-27 2024-06-24 Unilever Ip Holdings B V Use of an enzyme and surfactant for inhibiting microorganisms

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