WO2017200251A2 - Procédé et appareil d'obtention de peau de micro-cochon - Google Patents

Procédé et appareil d'obtention de peau de micro-cochon Download PDF

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Publication number
WO2017200251A2
WO2017200251A2 PCT/KR2017/005031 KR2017005031W WO2017200251A2 WO 2017200251 A2 WO2017200251 A2 WO 2017200251A2 KR 2017005031 W KR2017005031 W KR 2017005031W WO 2017200251 A2 WO2017200251 A2 WO 2017200251A2
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WO
WIPO (PCT)
Prior art keywords
skin
horseshoe
type hinge
pig
hinge member
Prior art date
Application number
PCT/KR2017/005031
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English (en)
Korean (ko)
Other versions
WO2017200251A3 (fr
Inventor
최선덕
최성준
강경수
조유미
Original Assignee
메디키네틱스 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Publication of WO2017200251A2 publication Critical patent/WO2017200251A2/fr
Publication of WO2017200251A3 publication Critical patent/WO2017200251A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N9/00—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
    • G01N9/24—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity by observing the transmission of wave or particle radiation through the material

Definitions

  • the present invention relates to a method for obtaining biological skin from a pig and a device used therein, and more particularly, to a method and a device for obtaining skin of a micropig used for skin absorption test.
  • the skin absorption test method as known through the 'in vitro skin absorption test guideline' published by the Korea Food and Drug Administration, is divided into in vivo test and in vitro test.
  • the former has the advantage of obtaining general information and metabolic information of the systemic circulation, but it is necessary to use live animals, it is difficult to distinguish the initial absorption phase, and there is a difference in permeability between humans and other species such as rats used in the experiment. I have a downside.
  • the latter is a method of measuring the amount of the test substance has passed through the skin to the solution reservoir, it can use human skin or other species of skin, repeated measurement of the test substance, do not use live animals
  • the range of test materials that can be used is broad, and there is an advantage in studying the relationship between skin damage and skin absorption that cannot be evaluated by in vivo testing for ethical reasons.
  • the in vitro skin absorption test can compare the skin absorption and permeation according to the composition of the material, it can be used as a useful model for evaluating the risk of skin absorption in the human body.
  • the former prior art relates to a skin transplantation method for facilitating the collection of skin tissue including an implant during skin transplantation of an animal, and there is no technique for obtaining thin skin to be used for in vitro skin absorption test.
  • a technique for obtaining thin skin including the dermal layer of pigs is used as a dressing material for the treatment of skin trauma patients such as burns, but after separating the entire skin layer that is attached to the subcutaneous fat, applying pressure using a medical peeler Since skin of a certain thickness is obtained from the epidermal layer, there is a problem that skin of uniform thickness can not be obtained according to the bending of the subcutaneous fat placed on the floor. To solve this problem, the subcutaneous fat must be removed manually by a knife or the like. There is a problem that takes a lot of time.
  • an object of the present invention is to provide a method for effectively obtaining a living skin of a pig used for skin absorption test and the like and a device used therefrom from a micro pig.
  • the skin obtaining method of the ultra-small pig is a pig preparation process, hair removal process, skin separation process, extraneous foreign material removal process, skin peeling process and skin standardization process sequentially
  • the pig is a miniature pig weighing less than 25kg
  • the skin separation process is to cut both ears in the order of neck, torso and hips in the state of laying the microscopic pig on its side, Including the head, the lower abdomen containing the four fingers, the front of the skin except the back of the butt including the tail is separated into a disc
  • the extra-skin foreign matter removal process is to cut the skin disc into a plurality of bands in a long shape with a predetermined width, the cut The plurality of strips of skin having a predetermined thickness from the epidermal layer using a predetermined skin separator. Characterized in that the removal, including the attached fat and / or flesh on the back of the skin.
  • the plurality of skin band pieces are another feature of the method for acquiring the skin of a micro pig according to the present invention, wherein the skin disc is cut in the same direction as the spine in a width of 5 to 7 cm.
  • the skin dermabrasion process is a microminiature according to the present invention to obtain a skin having a predetermined thickness from the epidermal layer with a medical dermabrasion with the back side of the skin facing the floor in a state where the skin backside of the skin strip is flattened by the process of removing extraneous foreign matter. It is another feature of the method for obtaining skin of a pig.
  • a predetermined elastic plate is placed on a table, and the back side of the skin of the skin strip piece is placed on the elastic plate, and then one side of the table is clipped with a lengthwise end of the skin strip piece.
  • the skin dermabrasion process is another feature of the method for acquiring the skin of a micro pig according to the present invention, wherein the skin separator proceeds simultaneously with the removal of extraneous foreign substances to directly obtain a skin having a predetermined thickness from the epidermal layer of the skin strip. .
  • the skin separator comprises: a bottom plate having two protruding support plates attached to each other at a predetermined distance in parallel; A gate-shaped support frame vertically wrapped outside the two protruding support plates and fastened in a height-adjustable manner; A blade fastened to an upper surface of the support frame; And a skin band piece guide member rotatably attached to the bottom plate to be spaced apart from the support frame.
  • the skin strip piece guide member is fixed to the bottom plate and the first horseshoe-type hinge member provided inside the two protruding support plate; Second horseshoe-type hinge members rotatably fastened to both side horizontal arms of the first horseshoe-type hinge member; And a first and second guide rod attached to the horizontal arm of the second horseshoe-type hinge member in parallel with the blade.
  • the first and second horseshoe-type hinge members have a predetermined width and have a shape bent in the longitudinal direction by the letter 'c', and both ends of the second horseshoe-type hinge members are formed on both side horizontal arms of the first horseshoe-type hinge member.
  • the first guide rod is fastened to the lower support rods on both side bushing protruding fixed perpendicularly to the horizontal arm of the second horseshoe-type hinge member away from the hinge axis
  • the second guide rod is smaller than the diameter of the first guide rod and is fastened with an upper push rod on both protruding arms rotatably fastened between the bushing of the first guide rod and the bent handle of the second horseshoe-type hinge member.
  • a horizontal arm of the second horseshoe-type hinge member parallel to the first guide rod between the hinge axis and the bushing of the first guide rod.
  • To the horizontal bar it is further attached, and in that the horizontal bar and the first horseshoe-shaped curved between the front protrusion has at least one spring member of the hinge member is fastened to a further feature of the skin, the separator according to the present invention.
  • the two protruding arms are rotatably fastened in contact with an inner side of the horizontal arm of the second horseshoe-type hinge member, and a rotational fastening handle is further fastened to one protruding arm of one side, and one end of the horizontal bar prevents reverse rotation. It is further provided that another feature of the skin separator according to the present invention.
  • the support frame is characterized in that the skin separator according to the present invention is further provided with a drive unit for driving the blade.
  • the present invention is to separate the front surface of the skin of the ultra-small pigs into discs, cut into a plurality of skin band pieces, and then effectively remove the subcutaneous fat and / or flesh spots attached to the back of the skin by using a skin separator, the pig of uniform thickness It is easy to mass produce skin.
  • FIG. 1 is a process flow chart of a method for obtaining skin of a micro pig according to an embodiment of the present invention.
  • FIG. 2 is a conceptual diagram showing an example of cutting and separating the skin in the skin separation process of the present invention and an example of cutting the skin disc into a plurality of strips of skin in a strip shape having a predetermined width in a process of removing extraneous foreign substances. .
  • FIG. 3 is a cross-sectional view taken along the line AA ′ of FIG. 2C.
  • Figure 4 is a perspective view of a skin separator according to an embodiment of the present invention, showing a state in which the second horseshoe-type hinge member is rotated to the rear.
  • FIG. 5 is an exploded perspective view of FIG. 4.
  • FIG. 6 is a perspective view illustrating a principle in which the second horseshoe-type hinge member is rotated forward by the elastic force of the spring member and the second guide rod is rotated by the rotation tightening knob after inserting the skin strip piece in FIG. 4.
  • FIG. 7 is a perspective view illustrating a state in which the second horseshoe-type hinge member and the second guide rod are rotated in FIG. 6, and then the skin band pieces are pulled forward and peeled.
  • FIG. 8 is a cross-sectional view illustrating a principle in which the second horseshoe-type hinge member and the second guide rod are rotated in FIG. 6, and a principle of removing or peeling off foreign substances of the skin strip.
  • FIG. 9 is a perspective view showing a skin peeling process of the present invention.
  • pig preparation step (S10), hair removal step (S20), skin separation step (S30), extraneous skin removal step (S40) , Skin peeling process (S50) and skin standardization process (S60) proceeds sequentially.
  • the pig preparation step (S10) checks the table to select the micro pigs (micropig) weighing less than 25kg, and confirm the cleanliness and condition of the skin to be finally confirmed as a skin providing pig.
  • Pigs that are confirmed as skin-providing pigs are anesthetized and checked for good anesthesia and ready for the next process.
  • the pig hair is firstly removed using an electric clipper, and the like, and a povidone scrub soap is applied to the body, and then the hair is secondarily removed using a razor. After all the pig's hair has been removed, wash it with water and dry it with a paper towel.
  • the skin disc 10 In the foreign skin removal step (S40), first, as shown in Figure 2 (c), take out the skin disc 10 contained in physiological saline and put it on a grid plate or engraved plate (not shown), the skin disc 10 is cut into a plurality of skin strip pieces 20 in a band shape with a predetermined width.
  • the plurality of skin strip pieces 20 is preferably such that the skin disc 10 is cut in the same direction (3) and the spine direction (2) in a width of 5 ⁇ 7cm.
  • the width of the skin strip piece 20 may vary depending on the size of the skin to be produced, but less than 5cm is not economical, if it exceeds 7cm will be out of the peelable area of the medical peeler, about 6cm is the most desirable.
  • Each cut skin strip 20 is divided into a skin layer 22 and a subcutaneous fat layer 24, as shown in FIG. 3, which is a cross-sectional view taken along line AA 'of FIG. 2 (c), and the skin layer 22 is an epidermal layer ( 22 ') and the dermis layer 22 ", the subcutaneous fat layer 24 is composed of subcutaneous fat 24' and flesh 24 "
  • the blade 300 of the skin separator including the subcutaneous fat 24 'and / or flesh 24' 'attached to the back of the skin to have a certain thickness from the epidermal layer 22' Depending on the height, part of the dermal layer 22 '' may be evenly removed.
  • the skin dermabrasion step (S50) is a work table 500 to face the back of the skin to the bottom, as shown in Figure 9, in the state that the back of the skin of the skin strip piece 20 is flattened by the step of removing extraneous foreign substances (S40). ) And a skin having a certain thickness from the epidermal layer 22 ′ with a medical dermatome 700.
  • the elastic plate 520 such as a rubber plate or a carpet is placed on the work table 500, and the back side of the skin of the skin strip piece 20 is placed on the elastic plate, and then the skin One end of the longitudinal strip of the belt piece is fixed to one side of the table 500 by the clip 600, and applied to the other end of the non-fixed longitudinal direction while applying a certain pressure to the medical peeler 700.
  • the skin layer 22 ′ peeled with the tweezers 800 can be pulled and peeled, in which case it can be effectively peeled to a uniform thickness without gripping.
  • the peeling thickness of the skin strip piece 20 is obtained by adjusting the medical peeling machine 700 to 300 ⁇ 1,200 ⁇ m, which may vary depending on the purpose of use of pig skin.
  • the skin dermabrasion process S50 may be performed simultaneously with the skin extraneous substance removal process S40 by the skin separator 1000, and the epidermal layer 22 ′ of the skin strip piece 20 may be formed. It is also possible to directly obtain the skin 22 having a certain thickness from the same.
  • the peeled skin is immersed in saline to prevent the surface from drying out.
  • the skin standardization process is to place the epidermal layer 22 'of the peeled skin face down and check that the thickness of the skin is constant, on the contrary, place the skin's epidermal layer 22' face upward and scratch the epidermis. Except for the part with a red dot, it is taken out by a predetermined
  • Pig skins placed in packaging containers will be stored frozen at minus 20 °C and supplied to each customer.
  • Skin separator 1000 according to an embodiment of the present invention, as shown in Figs. 4 and 5, the bottom plate 100, the two protruding support plate 110 is attached in parallel to a certain distance apart; A gate-shaped support frame 200 which is vertically wrapped with the bottom plate 100 outside the two protruding support plates and fastened to be adjustable in height; A blade 300 fastened to the upper surface of the support frame; And a skin strip piece guide member 400 spaced apart from the support frame and rotatably attached to the bottom plate 100.
  • the support frame 200 has a 'c' shape so that the iron plate having a width equal to or greater than the width of the blade 300 has a gate width 1.5 to 2 times larger than the width of the skin strip piece 20. It can be bent and made.
  • the two vertical pillar surfaces of the support frame 200 are fastened so that the height of each of the two projecting support plates 110 vertically attached to the bottom plate 100 is wrapped inward from the outside.
  • the supporting frame 200 may be fastened to the two protruding support plate 110 so that the height can be adjusted, as shown in Figure 5, the vertical long groove 210 on the two vertical pillar surface of the support frame 200 ) Are formed side by side in the same shape, and each of the two bolt through holes 112 are formed at positions corresponding to the vertical field grooves in the two protruding support plates 110, thereby forming the bolt 220 and the nut 222.
  • the height can be easily adjusted finely.
  • the blade 300 is fastened by the fastening means 310, 320 of the predetermined blade so as to slightly project rearward from the width of the support frame.
  • the fastening means of the blade 300 as shown in Figure 5, the blade attachment plate 310 and the bolt 320 and the nut 322 can be configured in the blade fixed type, a predetermined blade driving portion ( If not further provided) is attached to one side of the blade drive may be fastened to the blade to move left or right or rotate.
  • the blade 300 may be provided to be replaced at any time when necessary.
  • the skin strip piece guide member 400 as shown in Figures 4 and 5, the first horseshoe-type hinge member 410 is fixed to the bottom plate 100 and provided inside the two protruding support plate 110; Second horseshoe-type hinge members 420 rotatably fastened to both horizontal arms of the first horseshoe-type hinge member; And first and second guide rods 430 and 440 attached to the horizontal arm of the second horseshoe-type hinge member in parallel with the blade.
  • first and second horseshoe-type hinge members 410 and 420 are specifically, as shown in FIG. 5, each of which has a predetermined width and is bent into a 'c' in the longitudinal direction, and the second horseshoe-type hinge Both ends of the member 420 may be in contact with a plate on the inner side of both horizontal arms of the first horseshoe-type hinge member 410 and may rotate the bolt 422 fastened by the nut 424 to the through hole 421 by a hinge axis. Can be fastened.
  • the first horseshoe-shaped hinge member 410 is fixed to the bottom plate 100 by a bolt 130 and a nut 132 by the front middle connector 416, and the rear both sides of the horizontal arm and the bottom plate fixture 120
  • the bolt 412 and the nut 414 may be fastened to the through holes 122 and 411.
  • the first guide rods 430 as shown in Figure 8, to determine the thickness of the skin layer 22 to support the skin strip piece 20 from the bottom and peeled at intervals between the blade 300, A lower support rod is fastened to both side bushings 432 protruded and fixed perpendicularly to a horizontal arm of the second horseshoe-type hinge member 420 away from the hinge shaft 422.
  • the skin strip piece 20 is passed between the first guide rod 430 by pressing on the subcutaneous fat layer 24 of the skin strip piece (20).
  • the first guide rod 430 is smaller than the diameter of the first guide rod 430 and rotatably coupled between the bushing 432 of the first guide rod and the bent handle 420 of the second horseshoe-type hinge member. It is fastened to the upper push rod on both side protruding arm (444).
  • the two protruding arms 444 are in contact with the inside of the horizontal arm of the second horseshoe-type hinge member 420 to be rotatably fastened, and one side of the protruding arm further includes a rotary tightening knob 446 of the protruding arm 444. Can be fastened. 6 shows an example in which the protruding arm 444 is rotated in the opposite direction when the rotary tightening knob 446 is turned.
  • the horizontal arm 450 of the second horseshoe-type hinge member 420 is parallel to the first guide rod 430 between the hinge shaft 422 and the fastening portion of the bushing 432 of the first guide rod.
  • one or more spring members 452 may be fastened between the horizontal bar 450 and the bent front protrusion of the first horseshoe-type hinge member 410.
  • a reverse rotation preventing member 454 is further provided to rotate the second horseshoe-type hinge member 420 backward and forward the skin strip piece 20.
  • the second horseshoe-type hinge member 420 is rotated forward by the elastic force of the spring member 452. This is preferred.
  • the present invention provides a method for effectively obtaining the skin of pigs used for skin absorption test from a small pig and a device used therein, in particular a skin splitter that can effectively remove subcutaneous fat and flesh spots attached to the dermal layer of pigs.
  • the present invention relates to a method and an apparatus which can easily mass-produce a pig skin of uniform thickness, and thus have industrial applicability.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Surgical Instruments (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

La présente invention concerne un procédé d'obtention de la peau d'un micro-cochon et un appareil utilisé à cet effet, dans lequel une surface de peau entière d'un micro-cochon est séparée en disques de manière à être découpée en une pluralité de morceaux de bandes de peau, puis la graisse et/ou la chair sous-cutanée fixées au dos de la peau sont retirées efficacement au moyen d'un séparateur de peau, ce qui facilite la production en masse de peau de cochon d'une épaisseur uniforme.
PCT/KR2017/005031 2016-05-20 2017-05-16 Procédé et appareil d'obtention de peau de micro-cochon WO2017200251A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020160062293A KR101844034B1 (ko) 2016-05-20 2016-05-20 초소형 돼지의 피부 획득 방법 및 장치
KR10-2016-0062293 2016-05-20

Publications (2)

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WO2017200251A2 true WO2017200251A2 (fr) 2017-11-23
WO2017200251A3 WO2017200251A3 (fr) 2018-01-18

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Application Number Title Priority Date Filing Date
PCT/KR2017/005031 WO2017200251A2 (fr) 2016-05-20 2017-05-16 Procédé et appareil d'obtention de peau de micro-cochon

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KR (1) KR101844034B1 (fr)
WO (1) WO2017200251A2 (fr)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200238297Y1 (ko) 2001-04-13 2001-10-11 박상오 육류 절단기
US6659856B2 (en) * 2001-06-11 2003-12-09 John W. Long Automatic skin removal and fat trimming device for meat products II
KR20040027860A (ko) * 2004-03-15 2004-04-01 주식회사 청하기계 딥 스키닝 머시인
KR200361359Y1 (ko) * 2004-06-19 2004-09-08 최기호 생선 박피기
DE102007038365B4 (de) * 2007-08-11 2009-06-10 Nordischer Maschinenbau Rud. Baader Gmbh + Co Kg Vorrichtung und Verfahren zum Enthäuten von Fischfilets
KR101139434B1 (ko) * 2009-11-24 2012-04-27 주식회사 리온 돼지피부를 이용한 드레싱재의 제조방법
CA2782790C (fr) 2009-12-17 2018-01-02 Marel Meat Processing B.V. Systeme et procede de traitement d'animaux abattus et/ou d'elements de ceux-ci

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Publication number Publication date
KR101844034B1 (ko) 2018-03-30
WO2017200251A3 (fr) 2018-01-18
KR20170131090A (ko) 2017-11-29

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