WO2017113167A1 - 生物打印机喷头组件及生物打印机 - Google Patents

生物打印机喷头组件及生物打印机 Download PDF

Info

Publication number
WO2017113167A1
WO2017113167A1 PCT/CN2015/099822 CN2015099822W WO2017113167A1 WO 2017113167 A1 WO2017113167 A1 WO 2017113167A1 CN 2015099822 W CN2015099822 W CN 2015099822W WO 2017113167 A1 WO2017113167 A1 WO 2017113167A1
Authority
WO
WIPO (PCT)
Prior art keywords
nozzle
passage
bioprinter
outlet
material passage
Prior art date
Application number
PCT/CN2015/099822
Other languages
English (en)
French (fr)
Inventor
温学敏
李意军
王德明
Original Assignee
四川蓝光英诺生物科技股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 四川蓝光英诺生物科技股份有限公司 filed Critical 四川蓝光英诺生物科技股份有限公司
Priority to JP2018534155A priority Critical patent/JP6820933B2/ja
Priority to EP15911796.9A priority patent/EP3398777B1/en
Priority to US16/067,541 priority patent/US11161299B2/en
Priority to PCT/CN2015/099822 priority patent/WO2017113167A1/zh
Publication of WO2017113167A1 publication Critical patent/WO2017113167A1/zh

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
    • B29C64/205—Means for applying layers
    • B29C64/209—Heads; Nozzles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M35/00—Devices for applying media, e.g. remedies, on the human body
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B12/00—Arrangements for controlling delivery; Arrangements for controlling the spray area
    • B05B12/08—Arrangements for controlling delivery; Arrangements for controlling the spray area responsive to condition of liquid or other fluent material to be discharged, of ambient medium or of target ; responsive to condition of spray devices or of supply means, e.g. pipes, pumps or their drive means
    • B05B12/12—Arrangements for controlling delivery; Arrangements for controlling the spray area responsive to condition of liquid or other fluent material to be discharged, of ambient medium or of target ; responsive to condition of spray devices or of supply means, e.g. pipes, pumps or their drive means responsive to conditions of ambient medium or target, e.g. humidity, temperature position or movement of the target relative to the spray apparatus
    • B05B12/124—Arrangements for controlling delivery; Arrangements for controlling the spray area responsive to condition of liquid or other fluent material to be discharged, of ambient medium or of target ; responsive to condition of spray devices or of supply means, e.g. pipes, pumps or their drive means responsive to conditions of ambient medium or target, e.g. humidity, temperature position or movement of the target relative to the spray apparatus responsive to distance between spray apparatus and target
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/06—Spray pistols; Apparatus for discharge with at least one outlet orifice surrounding another approximately in the same plane
    • B05B7/061—Spray pistols; Apparatus for discharge with at least one outlet orifice surrounding another approximately in the same plane with several liquid outlets discharging one or several liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/16—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas incorporating means for heating or cooling the material to be sprayed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/112—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using individual droplets, e.g. from jetting heads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/30—Auxiliary operations or equipment
    • B29C64/386—Data acquisition or data processing for additive manufacturing
    • B29C64/393—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B13/00—Machines or plants for applying liquids or other fluent materials to surfaces of objects or other work by spraying, not covered by groups B05B1/00 - B05B11/00
    • B05B13/02—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work
    • B05B13/04—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work the spray heads being moved during spraying operation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B15/00—Details of spraying plant or spraying apparatus not otherwise provided for; Accessories
    • B05B15/50—Arrangements for cleaning; Arrangements for preventing deposits, drying-out or blockage; Arrangements for detecting improper discharge caused by the presence of foreign matter

Definitions

  • the present invention relates to the field of bioprinting, and more particularly to a bioprinter head assembly and a bioprinter.
  • Bio 3D printing refers to the printing of biological materials (including natural biomaterials and synthetic biomaterials or cell solutions) into a three-dimensional structure by 3D printing. It is different from ordinary 3D printing technology and bio 3D printing technology. Biological tissues or organs also have certain biological functions, which need to provide conditions for the further growth of cells and tissues. It is precisely because of the above characteristics that biological 3D printing technology is developing and facing many specific technical problems.
  • the technology of using cells as printing materials is called cell three-dimensional printing technology.
  • the prior art nozzle for bioprinting is a nozzle similar to a syringe needle that is mounted directly on a bioprinter.
  • the head device of such a bio-printer has a simple structure and is mainly used to fill active cells into the scaffold material, but cannot be packaged before printing.
  • the extrusion pressure and the friction of the nozzle sidewalls on the cells cause the cells in the bio-ink to be relatively damaged, so that the survival rate of the cells is generated.
  • Adverse effects which in turn affect the construction of biological constructs.
  • the technical problem solved by the present invention is to provide a bio-printer nozzle assembly and a bio-printer capable of simultaneously printing a plurality of printing materials.
  • a step it is also possible to combine a plurality of printing materials before printing, preferably by circumferentially wrapping the first material as the bio-ink with the second material, so as to protect the cells in the discharged bio-ink as much as possible. Not damaged.
  • a first aspect of the present invention provides a bioprinter head assembly, comprising: an outer nozzle having a second passage and an inner nozzle having a first passage, the inner nozzle being coaxially disposed at the In the two passages, the first passage forms a first material passage, the annular space between the outer nozzle and the inner nozzle forms a second material passage, and the second material passage is at an outlet of the first material passage Surrounding the first material passage for concentrating a second material ejected from the second material passage outlet toward a first material ejected from the first material passage outlet to form a bio-printed material Fluid printing unit.
  • the second flow path can guide and gradually converge the second material toward the first material, thereby concentrating the second material with the first material, for example, the second material is in the nozzle assembly.
  • the outlet material can evenly wrap the first material to form a higher quality fluid printing unit to protect the cells, thereby reducing cell damage caused by extrusion pressure and friction during printing, thereby improving cell survival. rate.
  • the bio-printer head assembly further includes a first material supply tube and an outer nozzle fixing portion, the outer nozzle being detachably coupled to the first outer nozzle fixing portion, the inner nozzle being detachably coupled to the first A material supply tube.
  • the outer nozzle and the inner nozzle are made detachable and can be separately replaced.
  • the disassembly is required, only the nozzle part of the nozzle assembly adjacent to the outlet needs to be removed, and only the partial parts are involved. Disassembly makes the replacement quicker.
  • the bioprinter showerhead assembly includes a set of nozzle kits that are comprised of a plurality of different gauge nozzles, the outer and inner nozzles being selected from the set of nozzle kits.
  • the nozzle assembly can spray different sizes of particles and different thicknesses of the wrapping layer to realize controllable wrapping.
  • the outlet of the outer nozzle is lower than the outlet of the inner nozzle.
  • the outer nozzle extension portion can be further tapered in a direction toward the outlet of the first material passage, thereby guiding the second material to continue to converge toward the direction of the first material, so as to more reliably and fully wrap the first material.
  • the outer nozzle includes a first guiding portion
  • the inner nozzle includes a second guiding portion
  • the first guiding portion and the second guiding portion are disposed adjacent to an outlet of the showerhead assembly, the first The first annular space between the guide and the second guide is tapered in a direction toward the first material passage exit.
  • the second material passage is tapered in a direction toward the outlet of the first material passage adjacent to the outlet, and the second material can be guided to further converge toward the first material during the spraying process, Thereby, the first material is wrapped at the exit of the first material passage, thereby minimizing damage to the first material due to mechanical friction.
  • the outer nozzle further includes a first body portion connected to the first guiding portion, the inner nozzle further including a second body portion connected to the second guiding portion, the first body portion and The second annular space between the second body portions is tapered at least partially toward the second material passage outlet.
  • the annular space between the first body portion and the second body portion is designed to be at least partially tapered, and the pressure of the second material can be further increased to increase the flow rate, thereby making the second material
  • the flow in the second material passage is smoother, less prone to clogging, and the wrapping of the first material at the outlet of the first material passage is more sufficient and uniform.
  • first material passage in the first annular space is elongated, and a portion of the first material passage in the second annular space is at least partially directed toward the first material passage outlet The direction is gradual.
  • the first material passageway is designed as an elongated passage adjacent the outlet to direct the first material to be more smoothly ejected to be wrapped by the second material.
  • the present invention provides a second aspect to provide a living being A printer comprising the bioprinter head assembly of the above embodiments.
  • the bio-printer is capable of maintaining high activity and long life for various biological constructs produced by obtaining high-quality bio-printing materials.
  • the bioprinter further includes a pressure regulating device for controlling fluid pressure within the first material passage and the second material passage.
  • the bioprinter further includes temperature control means for controlling the temperature of the first material passage and the second material passage.
  • the temperature control device by setting the temperature control device, it is advantageous to maintain the activity of the biological material during the printing process, especially when the bio-printing material with high viscosity is used, the fluidity can be improved, and the temperature is lowered as much as possible. This causes the nozzle to clog, which improves printing efficiency.
  • the bioprinter further includes position detecting means for determining an initial print position of the head unit.
  • the bio-printer nozzle assembly of the present invention adopts a double-layer coaxial arrangement of inner and outer nozzles, and the first passage of the inner nozzle forms a first material passage, and the annular space between the outer nozzle and the inner nozzle Forming a second material passage that enables the second material ejected from the outlet of the second material passage to enclose the first material ejected from the outlet of the first material passage to form a fluid printing unit of the bioprinting material to protect the cells, thereby reducing In the printing process, the cell damage caused by the extrusion pressure and friction, thereby improving the cell survival rate, has high reliability; and the second material channel is coaxially surrounded by the outlet adjacent to the first material channel.
  • the form of the material passage is beneficial to make the second material more evenly wrapped around the first material, avoiding The phenomenon of uneven thickness is not formed to form a high quality fluid printing unit.
  • FIG. 1 is a schematic structural view of an embodiment of a bio-printer nozzle assembly of the present invention
  • Figure 2 is an enlarged view of the nozzle portion of the bioprinter head assembly of Figure 1;
  • FIG. 3 is a schematic view showing a state in which a fluid printing unit flows out of the bioprinter head unit shown in FIG. 1;
  • FIG. 4 is a schematic structural view of an embodiment of a bio-printer of the present invention.
  • FIG. 5 is a schematic structural view of a temperature control device in the bioprinter shown in FIG. 4.
  • FIG. 5 is a schematic structural view of a temperature control device in the bioprinter shown in FIG. 4.
  • the nozzle assembly of the bio-printer cannot package the cells before printing, which may cause damage to the cells during printing.
  • the present invention provides a bio-printer nozzle assembly, the structure of which is shown in FIG. 1 and 2 shows the schematic.
  • an outer nozzle 1 having a second passage and an inner nozzle 2 having a first passage, the inner nozzle 2 being coaxially disposed in the second passage, the first passage forming the first material passage A,
  • the annular space between the outer nozzle 1 and the inner nozzle 2 forms a second material passage B, the second material passage B surrounding the first material passage A at an outlet adjacent to the first material passage A for making the second material passage B
  • the second material ejected from the outlet converges toward the first material ejected from the outlet of the first material passage A to form a fluid printing unit of the bioprinting material.
  • the first material channel A and the second material channel B can be used to transport the same kind of materials, and are also used to transport different kinds of materials, can be used simultaneously, or can be used independently of each other.
  • the first material and the second material may be the same or different.
  • the first material may be, for example, a dispersed phase solution (homogeneous continuous fluid) or a main material containing a suspension of cells (non-homogeneous fluid), and the second material may be, for example, Auxiliary material for sheath fluid or nutrient solution.
  • the fluid printing unit is a component printing unit of the bioprinting material, and may be formed, for example, by wrapping the main material with the auxiliary material.
  • the bio-printer nozzle assembly of the present invention adopts a double-layer coaxial arrangement of inner and outer nozzles, and the first passage of the inner nozzle forms a first material passage, and the annular space between the outer nozzle and the inner nozzle forms a second material passage, which enables
  • the auxiliary material ejected from the outlet of the second material passage wraps the main material ejected from the outlet of the first material passage to protect the cells by the fluid printing unit forming the bio-printing material, thereby reducing the extrusion pressure during the printing process and Friction causes cell damage, thereby improving cell survival rate, and has high reliability; and the second material channel coaxially surrounds the first material channel in an exit adjacent to the first material channel, facilitating the auxiliary material More evenly wrapped around the main material, try to avoid the phenomenon of uneven thickness to form a higher quality fluid printing unit.
  • the external spray can be used to facilitate cleaning of the nozzle assembly.
  • the nozzle 1 and the inner nozzle 2 are configured to be detachable and replaceable separately, so that only the nozzle portion of the nozzle assembly adjacent to the outlet needs to be removed when it is required to be disassembled, and only the disassembly of the partial parts is involved, so that the replacement is quicker.
  • the bio-printer head assembly further includes a first material supply tube 4 and an outer nozzle fixing portion rod 5, and the outer nozzle 1 is detachably coupled to the outer nozzle fixing portion. 5.
  • the inner nozzle 2 is detachably coupled to the first material supply pipe 4. Since there is an annular space between the first material supply tube 4 and the outer nozzle fixing portion 5, in order to achieve relative fixation of the first material supply tube 4 and the outer nozzle fixing portion 5, a connecting member 6 may be provided at the upper end of the outer nozzle fixing portion 5. The connecting piece 6 cooperates with the first material supply pipe 4.
  • the outer nozzle 1 and the inner nozzle 2 are respectively screwed to the outer nozzle fixing member 5 and the first material supply tube 4, and the disassembly and assembly can be realized by rotating the two nozzles, and the connection manner is relatively reliable and does not need to be Add additional extra parts to aid the connection.
  • those skilled in the art can also implement a detachable connection by using a quick-release mechanism, and the nozzle can be disassembled and assembled only by pressing or releasing the locking member.
  • a nozzle kit set comprising a plurality of different outer nozzles 1 and inner nozzles is included 2
  • Composition when performing a printing task, a suitable outer nozzle 1 and inner nozzle 2 can be selected from the nozzle kit group.
  • Different specifications mean that the shape, size or taper of the nozzle is different, but the size of the threaded joint is the same. In this way, nozzles of different specifications can be changed at any time as needed, and different aperture and taper fits can be obtained to provide fluid printing units of different sizes, or to obtain better wrapping effects.
  • the primary and secondary materials are wrapped at the outlet of the showerhead assembly.
  • the outlet of the outer nozzle 1 is flush with the outlet of the inner nozzle 2. More preferably, at the outlet of the second material passage B, the outlet of the nozzle 2 in the outlet of the outer nozzle 1 such that the extension portion can be further tapered in a direction toward the outlet of the first material passage A, thereby guiding the auxiliary material further toward The side of the first material Converging to make the main material more reliable and fully wrapped.
  • the first material passage A is an elongated passage adjacent the nozzle assembly outlet, enabling the orientation and alignment of the primary material as it is ejected, reducing the likelihood of clogging.
  • the portion of the second material passage B adjacent to the outlet is tapered in a direction toward the outlet of the first material passage A, which is capable of applying pressure to the auxiliary material in a direction toward the flow of the main material to guide the auxiliary material toward the main material.
  • the direction is sprayed to better wrap the main material, and the pressure on the auxiliary material can be increased to increase the flow rate, so that the auxiliary material flows out more smoothly to realize the wrapping.
  • One skilled in the art can design a controllable package prior to printing by designing the extent to which the second material channel is tapered.
  • the outer nozzle 1 includes a first guiding portion 11 and a first main body portion 12, and the inner nozzle 2 includes a second portion.
  • the guiding portion 21 and the second body portion 22, the first guiding portion 11 and the second guiding portion 21 are disposed adjacent to an outlet of the head assembly, and an annular space between the first guiding portion 11 and the second guiding portion 21 is along the direction The direction of the outlet of the first material passage A is tapered.
  • the cross section of the portion of the second material passage B adjacent to the outlet is tapered, and the tapered tapered form has a better guiding effect on the fluid than other shapes, and the auxiliary material can be at the same height.
  • the flow rates are the same so that the flow rate to the outlet of the second material passage is also substantially the same, thereby enabling the auxiliary material to evenly wrap the main material.
  • the second guiding portion 21 is cylindrical and the first guiding portion 11 is inverted conical.
  • the first guiding portion 11 and the second guiding portion 21 may each be designed as an inverted cone, and the taper of the first guiding portion 11 is greater than the taper of the second guiding portion 21.
  • the first body portion 12 includes a first cylindrical portion and a first conical portion, the first conical portion being coupled between the first guiding portion 11 and the first cylindrical portion, the first cylindrical portion being threadedly engaged Or other fastening means are connected to the outer nozzle fixing portion 5, and the outer nozzle fixing portion 5 is provided with a connecting pipe 3 for introducing the auxiliary material into the second material passage B.
  • the second body portion 22 includes a second cylindrical portion and a second conical portion, the second conical portion being coupled between the second guiding portion 21 and the second cylindrical portion, the second cylindrical portion being threadedly fitted or It is fastened to the first material supply pipe 4.
  • the second conical portion functions as a transition, and the cross-sectional area of the first material passage A is changed from large to small, so that the main material is converted into a fluid printing unit that satisfies printing requirements when flowing out, and the first conical portion is designed for It is adapted to the second conical portion to guide the auxiliary material.
  • the space between the first conical portion and the second conical portion of the second material passage B is tapered, and the pressure of the auxiliary material can be further increased to increase the flow rate, so that the auxiliary material is The flow in the second material passage B is smoother, less prone to clogging, and the wrapping of the main material at the outlet is more sufficient.
  • the first material passage A corresponding to the second guiding portion 21 is an elongated passage, and the direction of the first material passage A corresponding to the first main body portion 12 at least partially toward the outlet of the first material passage A is Tapered to achieve a transition to the elongated channel.
  • the first material passage A corresponding to the second cylindrical portion is a cylindrical passage having a large cross-sectional area, and the second guide portion is realized by the transition of the second conical portion.
  • the corresponding first material passage A of 21 is an elongated cylindrical passage.
  • the elongated passage is preferably provided for a single row of main materials (e.g., cells) to guide the main material to be more smoothly ejected to be wrapped by the auxiliary material to form a bioprinting material that satisfies the size requirement.
  • main materials e.g., cells
  • the main material flows along the first material passage A
  • the auxiliary material flows along the tapered second material passage B, and has a certain pressure, and adheres to reach when reaching the outlet of the second material passage B.
  • the present invention also provides a bio-printer, such as the embodiment shown in Figures 4 and 5, comprising the above-described bio-printer head assembly, wherein the bio-printer head assembly of the present invention is capable of wrapping cells during printing, thereby The cells are protected from damage and have a high survival rate, so the bio-printer using the nozzle assembly has the corresponding beneficial technical effects, and can obtain various biological constructs by obtaining high-quality bio-printing materials. Can maintain good biological properties, such as higher activity and longer life.
  • the bioprinter is a 3D bioprinter
  • the bioprinter head assembly of the present invention is particularly suitable for use in 3D bioprinters.
  • the bioprinter further includes a fixing plate 7, a mounting bracket 8, a first material supply source 31, and a second material supply source 32, and the fixing plate 7 serves as a reference for each component in the entire bioprinter.
  • the mounting bracket 8 is disposed at a lower middle portion of the fixing plate 7 , and the first material supply source 31 and the second material supply source 32 are respectively configured to accommodate the main material and the auxiliary material, and are arranged side by side in the mounting bracket 8 .
  • the material supply source can be a container that holds the material.
  • the nozzle assembly P (see FIG. 4) of the present invention is mounted directly below the first material supply source 31 such that the first material supply source 31 is in communication with the first material passage A, the second material supply source 32 and the second material passage B It is connected through the connecting pipe 3.
  • the bioprinter it is necessary to achieve the flow of the bioprinting material by pressure control, preferably air pressure control, and thus the bioprinter needs to include a pressure regulating device such as an electric proportional valve.
  • a pressure regulating device such as an electric proportional valve.
  • the materials are loaded in the first material supply source 31 and the second material supply source 32, respectively, they are respectively connected with the electric proportional valves in the gas path, and the corresponding electric proportional valves of the two material supply sources are simulated in real time with the control system.
  • the quantity communication is controlled by the upper software to control the extrusion amount of the two material supply sources.
  • the discharge speed of the fluid in the first material passage A and the second material passage B can be controlled by adjusting the air pressure separately.
  • a main material is added to the first material supply source 31, and the main material is a mixed substance containing cells, and the state may be a fluid having fluidity or a gel state;
  • the auxiliary material is added to the second material supply source 32, and the auxiliary material may be the same material as the main material (main and auxiliary materials), and the auxiliary material may be a substance for supplying nutrients or a substance for providing adhesion according to the use requirement. (It can also be used to form a protective layer), and it may be a substance that coats around the main material to form a protective layer.
  • the main material can be continuously ejected, and the outer surface is wrapped by the auxiliary material to form an elongated structure as a whole.
  • a main material may be added in the first material supply source 31, an auxiliary material may be added in the second material supply source 32, and the first material is added.
  • the supply source 31 frequently pressurizes and opens and closes to form a pulse for controlling the main material to be ejected as discrete particles from the outlet of the first material passage A; and applies a continuous pressure to the second material supply source 32 for control
  • the auxiliary material is continuously ejected from the outlet of the second material passage B, and of course the pulse pressure can be applied to the second material supply source 32.
  • Fig. 3 by controlling the pulse of the main material, discrete particles can be formed and combined with the outer auxiliary material to form a fluid printing unit similar to egg yolk egg white, and rapidly cooled and shaped under the action of temperature.
  • the bioprinter of the present invention further includes a temperature control device 9 for controlling the temperature of the main material and the auxiliary material, and the first material supply source 31 and the second material supply source 32 may employ independent temperature control devices.
  • the temperature control device 9 is disposed on a side of the mounting support 8 away from the fixed plate 7.
  • the temperature control device 9 includes, in order from the end of the mounting support 8, a cooling fin 91, a heat sink 92, and a cooling fan 93.
  • the cooling fin 91 is disposed near the surface of the mounting support 8, and the bio-printing material can be heated or cooled under the control of the external temperature controller.
  • the refrigerating sheet 91 can select the semiconductor refrigerating sheet, the temperature-control end of the semiconductor refrigerating sheet and the material supply.
  • the heat source is used to accelerate the heat dissipation process of the heat sink 92.
  • the heat sink 92 is used to remove the heat generated by the semiconductor cooling sheet during operation.
  • the heat sink fan 93 is used to accelerate the heat dissipation process of the heat sink 92. Further, it is also possible to provide temperature detecting means outside the first material supply source 31 and the second material supply source 32 to make a more accurate judgment of the current temperatures of the main material and the auxiliary material.
  • the temperature control device 9 by setting the temperature control device 9, it is advantageous to maintain the activity of the biological material during the printing process, especially when the bio-printing material with high viscosity is used, the fluidity can be improved, and the nozzle clogging due to the low temperature is avoided as much as possible. Phenomenon, which improves printing efficiency.
  • the bio-printer further includes a position detecting device 13 disposed in the chute 33 mounted on the fixed plate 7, and the position detecting device 13 can be moved up and down along the chute 33. The height of the printing platform is judged prior to printing to determine the initial printing position of the head assembly, thereby achieving higher printing accuracy.

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Manufacturing & Machinery (AREA)
  • Optics & Photonics (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Anesthesiology (AREA)
  • Coating Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Nozzles (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

提供了一种生物打印机喷头组件及生物打印机，其中喷头组件包括具有第二通道的外喷嘴（1）和具有第一通道的内喷嘴(2)。内喷嘴同轴设在第二通道内，第一通道形成第一材料通道（A），外喷嘴和内喷嘴之间的环形空间形成第二材料通道(B)，第二材料通道在第一材料通道的出口处环绕第一材料通道，用于使从第二材料通道出口喷出的第二材料朝向从第一材料通道出口喷出的第一材料汇聚，以形成流体打印单元。这种喷嘴组件能够使第二材料在喷头组件的出口处均匀地包裹第一材料，以形成质量较高的流体打印单元，以对细胞进行保护，从而减少在打印过程中由于受到挤出压力和摩擦力而造成细胞损伤，进而提高细胞的存活率。

Description

生物打印机喷头组件及生物打印机 技术领域
本发明涉及生物打印领域，尤其涉及一种生物打印机喷头组件及生物打印机。
背景技术
生物3D打印是指通过3D打印的原理和方法，将生物材料(包括天然生物材料和合成生物材料或细胞溶液)打印成为设计的三维结构体，区别于普通的3D打印技术，生物3D打印技术生产的生物组织或器官还具有一定的生物学功能，需为细胞和组织的进一步生长提供条件，正是由于上述特性，生物3D打印技术在发展中，面临着很多特定的技术问题。
其中，在生物3D打印领域，将细胞作为打印材料的技术称为细胞三维打印技术，人们可以利用细胞和生物相容性材料制成生物墨汁，喷头移动并将生物墨汁喷出，通过程序控制喷头运动打印生物墨水，将生物墨汁按照预设的目标打印物体的三维构建数字模型打印成型。
现有技术中用于生物打印的喷头为类似于注射器针头的喷嘴，该喷嘴直接安装在生物打印机上面。此种生物打印机的喷头装置结构简单，主要用来将活性细胞填入支架材料中，但是无法在打印之前实现包裹。而且，在采用喷头直接将细胞喷出至打印平台的过程中，挤出压力和喷嘴侧壁对细胞的摩擦力会使生物墨汁中的细胞受到比较大的损伤，从而对细胞的存活率会产生不利影响，进而影响生物构建体的构建。
发明内容
为克服以上技术缺陷，本发明解决的技术问题是提供一种生物打印机喷头组件及生物打印机，能够将多种打印材料同时打印，进 一步地，也可以使得多种打印材料在打印前组合在一起，优选地用第二材料对作为生物墨汁的第一材料进行周向包裹，使其也具有保护喷出的生物墨汁中的细胞尽量不受损伤。
为解决上述技术问题，本发明第一方面提供了生物打印机喷头组件，其特征在于，包括具有第二通道的外喷嘴和具有第一通道的内喷嘴，所述内喷嘴同轴设在所述第二通道内，所述第一通道形成第一材料通道，所述外喷嘴和所述内喷嘴之间的环形空间形成第二材料通道，所述第二材料通道在所述第一材料通道的出口处环绕着所述第一材料通道，用于使从所述第二材料通道出口喷出的第二材料朝向从所述第一材料通道出口喷出的第一材料汇聚，以形成生物打印材料的流体打印单元。
在该基本的技术方案中，第二流道能够将第二材料朝着第一材料的方向引导并逐渐汇聚，从而将第二材料与第一材料汇聚在一起，例如第二材料在喷头组件的出口处能够均匀地包裹第一材料，以形成质量较高的流体打印单元，以对细胞进行保护，从而减少在打印过程中由于受到挤出压力和摩擦力而造成细胞损伤，进而提高细胞的存活率。
进一步地，生物打印机喷头组件还包括第一材料供应管和外喷嘴固定部，所述外喷嘴可拆卸地连接至所述第一外喷嘴固定部，所述内喷嘴可拆卸地连接至所述第一材料供应管。
在该改进的技术方案中，将外喷嘴和内喷嘴做成可拆卸并能单独更换的结构，在需要拆卸时只需要将喷头组件邻近出口的喷嘴部分拆下即可，仅涉及到局部零件的拆卸，使得更换较为快捷。
进一步地，生物打印机喷头组件包括由多个不同规格的喷嘴组成的喷嘴套件组，所述外喷嘴和所述内喷嘴从所述喷嘴套件组中选出。
在该改进的技术方案中，通过更换不同规格的内喷嘴和外喷嘴，就能够使喷头组件喷出不同尺寸的颗粒和不同厚度的包裹层，以实现可控包裹。
进一步地，在所述第二材料通道的出口处，所述外喷嘴的出口低于所述内喷嘴的出口。
在该改进的技术方案中，外喷嘴延长部分能够进一步沿着朝向第一材料通道出口的方向渐缩，从而引导第二材料继续向第一材料的方向汇聚，以便更加可靠和充分地包裹第一材料。
进一步地，所述外喷嘴包括第一引导部，所述内喷嘴包括第二引导部，所述第一引导部和所述第二引导部邻近于所述喷头组件的出口设置，所述第一引导部和所述第二引导部之间的第一环形空间沿着朝向所述第一材料通道出口的方向是渐缩的。
在该改进的技术方案中，第二材料通道在邻近于出口处沿着朝向第一材料通道出口的方向是渐缩的，可以引导第二材料在喷出的过程中进一步向第一材料汇聚，从而在第一材料通道的出口处对第一材料进行包裹，从而尽量避免第一材料喷出时由于机械摩擦力的作用而受到损伤。
进一步地，所述外喷嘴还包括与所述第一引导部连接的第一主体部，所述内喷嘴还包括与所述第二引导部连接的第二主体部，所述第一主体部和所述第二主体部之间的第二环形空间至少部分朝向所述第二材料通道出口的方向是渐缩的。
在该改进的技术方案中，将第一主体部和第二主体部之间的环形空间设计为至少部分渐缩的形式，可以进一步增加第二材料的压力以增大流速，从而使第二材料在第二材料通道内流动更顺畅，不容易堵塞，而且在第一材料通道出口处对第一材料的包裹更加充分、均匀。
进一步地，所述第一材料通道在所述第一环形空间中的部分为细长的，所述第一材料通道在所述第二环形空间中的部分至少局部朝向所述第一材料通道出口的方向是渐缩的。
在该改进的技术方案中，将第一材料通道邻近于出口处设计为细长通道，能够引导第一材料更顺畅地喷出以被第二材料包裹。
为解决上述技术问题，本发明提供了第二方面提供了一种生物 打印机，包括上述实施例所述的生物打印机喷头组件。
在该基本的技术方案中，生物打印机能够通过获得优质的生物打印材料，从而使制作的各种生物构建体保持较高的活性和较长的寿命。
进一步地，生物打印机还还包括压力调节装置，所述压力调节装置用于控制所述第一材料通道和所述第二材料通道内的流体压力。
在该改进的技术方案中，通过设置压力调节装置，能够控制第一材料和第二材料以不同的速度从出口喷出。
进一步地，生物打印机还包括用于控制所述第一材料通道和所述第二材料通道的温度的温控装置。
在该改进的技术方案中，通过设置温控装置，有利于在打印过程中保持生物材料的活性，尤其是在采用高黏度的生物打印材料时，能够提高流动性，尽量避免由于温度较低而导致喷嘴堵塞的现象，从而提高打印效率。
进一步地，生物打印机还包括用于对所述喷头组件的初始打印位置进行判断的位置检测装置。
在该改进的技术方案中，通过设置位置检测装置，能够在打印前对打印平台的高度进行判断，以确定喷头组件的初始打印位置，从而获得较高的打印精度。
由此，基于上述技术方案，本发明的生物打印机喷头组件采用内外喷嘴双层同轴设置的结构形式，且内喷嘴的第一通道形成第一材料通道，外喷嘴和内喷嘴之间的环形空间形成第二材料通道，能够使从第二材料通道出口喷出的第二材料包裹从第一材料通道出口喷出的第一材料，以形成生物打印材料的流体打印单元对细胞进行保护，从而减少在打印过程中由于受到挤出压力和摩擦力而造成细胞损伤，进而提高细胞的存活率，具有较高的可靠性；而且第二材料通道在邻近于第一材料通道的出口同轴环绕第一材料通道的形式，有利于使第二材料更加均匀地包裹在第一材料之外，尽量避 免薄厚不均的现象，以形成质量较高的流体打印单元。
附图说明
此处所说明的附图用来提供对本发明的进一步理解，构成本申请的一部分，本发明的示意性实施例及其说明仅用于解释本发明，并不构成对本发明的不当限定。在附图中：
图1为本发明生物打印机喷头组件的一个实施例的结构示意图；
图2为图1所示生物打印机喷头组件的喷嘴部分的放大图；
图3为图1所示生物打印机喷头组件中流体打印单元流出的状态示意图；
图4为本发明生物打印机的一个实施例的结构示意图；
图5为图4所示生物打印机中温控装置的结构示意图。
附图标记说明
1－外喷嘴；11－第一引导部；12－第一主体部；2－内喷嘴；21－第二引导部；22－第二主体部；3－连接管；4－第一材料供应管；5－外喷嘴固定部；6－连接件；7－固定板；8－安装支座；9－温控装置；91－制冷片；92－散热器；93－散热风扇；31－第一材料供应源；32－第二材料供应源；33－滑槽；13－位置检测装置；A－第一材料通道；B－第二材料通道。
具体实施方式
下面通过附图和实施例，对本发明的技术方案做进一步的详细描述。
本发明的具体实施方式是为了便于对本发明的构思、所解决的技术问题、构成技术方案的技术特征和带来的技术效果有更进一步的说明。需要说明的是，对于这些实施方式的说明并不构成对本发明的限定。此外，下面所述的本发明的实施方式中涉及的技术特征只要彼此之间未构成冲突就可以相互组合。
现有技术中生物打印机的喷头组件无法在打印之前对细胞实现包裹，会导致在打印过程中造成细胞的损伤，为此，本发明提供了一种生物打印机喷头组件，其结构参考图1和图2所示的示意图。在一个示意性的实施例中，包括具有第二通道的外喷嘴1和具有第一通道的内喷嘴2，内喷嘴2同轴设在第二通道内，第一通道形成第一材料通道A，外喷嘴1和内喷嘴2之间的环形空间形成第二材料通道B，第二材料通道B在邻近于第一材料通道A的出口环绕第一材料通道A，用于使从第二材料通道B出口喷出的第二材料朝着从第一材料通道A出口喷出的第一材料汇聚，以形成生物打印材料的流体打印单元。
第一材料通道A和第二材料通道B可以用于输送同种类的材料，也是用于输送不同种类材料，可以同时使用，也可以各自独立地使用。第一材料和第二材料可以相同也可以不同。
当第一材料和第二材料不同时，第一材料可以例如为分散相溶液(均质的连续流体)或者含有细胞的悬液(非均质的流体)的主材，第二材料可以例如为四周鞘液或营养液的辅材。流体打印单元是生物打印材料的组成打印单元，例如可以由辅材包裹主材而形成。
本发明的生物打印机喷头组件采用内外喷嘴双层同轴设置的结构形式，且内喷嘴的第一通道形成第一材料通道，外喷嘴和内喷嘴之间的环形空间形成第二材料通道，能够使从第二材料通道出口喷出的辅材包裹从第一材料通道出口喷出的主材，以形成生物打印材料的流体打印单元对细胞进行保护，从而减少在打印过程中由于受到挤出压力和摩擦力而造成细胞损伤，进而提高细胞的存活率，具有较高的可靠性；而且第二材料通道在邻近于第一材料通道的出口同轴环绕第一材料通道的形式，有利于使辅材更加均匀地包裹在主材之外，尽量避免薄厚不均的现象，以形成质量较高的流体打印单元。
由于打印机在使用时需要定期对喷头组件内部进行清理，因而在一个改进的实施例中，为了便于对喷头组件进行清理，可将外喷 嘴1和内喷嘴2做成可拆卸并能单独更换的结构，这样在需要拆卸时只需要将喷头组件邻近出口的喷嘴部分拆下即可，仅涉及到局部零件的拆卸，使得更换较为快捷。
为实现这一目的，可以采用如图1所示的具体结构，生物打印机喷头组件还包括：第一材料供应管4和外喷嘴固定部杆5，外喷嘴1可拆卸地连接到外喷嘴固定部5，内喷嘴2可拆卸地连接在第一材料供应管4上。由于第一材料供应管4和外喷嘴固定部5之间存在环形空间，因而为了实现第一材料供应管4和外喷嘴固定部5的相对固定，可以在外喷嘴固定部5的上端设置连接件6，连接件6与第一材料供应管4相配合。
可选地，外喷嘴1和内喷嘴2分别与外喷嘴固定件5和第一材料供应管4通过螺纹连接，通过旋转两个喷嘴即可实现拆装，这种连接方式较为可靠，也不需要增加其它额外的零件来辅助连接。除了螺纹连接，本领域技术人员还可以采用快插机构实现可拆卸连接，只需要按动或者释放锁定件即可实现喷嘴的拆装。
为了使喷头组件能够喷出不同规格的颗粒和不同厚度包裹液，在喷嘴可拆卸的实施例的基础上，包括一个喷嘴套件组，该喷嘴套件组由多个不同规格的外喷嘴1和内喷嘴2组成，在进行一项打印任务时，可以从喷嘴套件组中选出合适的外喷嘴1和内喷嘴2。规格不同是指喷嘴的形状、尺寸或者锥度不同，但是螺纹连接处的尺寸相同。这样就可以根据需要随时更换不同规格的喷嘴，得到不同的孔径和锥度的配合，以提供不同尺寸的流体打印单元，或者获得较佳的包裹效果。由此，通过更换不同规格的外喷嘴1和内喷嘴2，就能够实现辅材对主材的可控包裹。
对于上述的实施例，主材和辅材会在喷头组件的出口处进行包裹。一般地，在第二材料通道B的出口处，外喷嘴1的出口与内喷嘴2的出口相齐平。更优地，在第二材料通道B的出口处，外喷嘴1的出口内喷嘴2的出口，这样延长部分能够进一步沿着朝向第一材料通道A出口的方向渐缩，从而引导辅材进一步向第一材料的方 向汇聚，以便更加可靠和充分地包裹主材。
在一种优选的结构形式中，第一材料通道A在邻近喷头组件出口为细长通道，能够使主材在喷出时进行定向引导和排序，降低堵塞的可能性。第二材料通道B邻近于出口的部分沿着朝向第一材料通道A出口的方向是渐缩的，这样能够给辅材沿着朝向主材流动的方向施加压力，以引导辅材向着主材的方向喷出，从而更好地包裹主材，而且还能增加辅材受到的压力以提高流速，从而使辅材更加顺畅地流出以实现包裹。本领域技术人员可以通过设计第二材料通道渐缩的程度，以在打印之前实现可控的包裹。
下面将针对第二材料通道B渐缩的实施例给出一种具体的实现结构，如图2所示，外喷嘴1包括第一引导部11和第一主体部12，内喷嘴2包括第二引导部21和第二主体部22，第一引导部11和第二引导部21邻近于喷头组件的出口设置，第一引导部11和第二引导部21之间的环形空间沿着朝向所述第一材料通道A出口的方向是渐缩的。
更优地，第二材料通道B邻近于出口的部分的剖面是锥形的，锥形渐缩的形式相比于其它形状对流体具有较好的引导作用，而且能使辅材在同一高度上的流速相同，从而使到达第二材料通道出口处的流速也基本相同，进而实现辅材均匀地对主材进行包裹。例如，在图1所示的实施例中，第二引导部21为柱形，第一引导部11为倒锥形。在其它的实施例中，也可以将第一引导部11和第二引导部21均设计为倒锥形，且第一引导部11的锥度大于第二引导部21的锥度。
在该具体的结构形式中，第一主体部12包括第一圆柱部和第一圆锥部，第一圆锥部连接在第一引导部11和第一圆柱部之间，第一圆柱部通过螺纹配合或其它紧固方式与外喷嘴固定部5连接，外喷嘴固定部5上设有连接管3，用于将辅材引入第二材料通道B。第二主体部22包括第二圆柱部和第二圆锥部，第二圆锥部连接在第二引导部21和第二圆柱部之间，第二圆柱部通过螺纹配合或其 它紧固方式与第一材料供应管4连接。其中，第二圆锥部起到过渡作用，将第一材料通道A的截面积由大变小，从而使主材在流出时转变为满足打印要求的流体打印单元，第一圆锥部的设计是为了与第二圆锥部相适应，从而对辅材起到引导作用。作为一种更优的设计形式，第二材料通道B在第一圆锥部和第二圆锥部之间的空间是渐缩的，可以进一步增加辅材的压力以增大流速，从而使辅材在第二材料通道B内流动更顺畅，不容易堵塞，而且在出口处对主材的包裹更加充分。
对于第一材料通道A，与第二引导部21对应的第一材料通道A是细长通道，与第一主体部12对应的第一材料通道A至少部分朝向第一材料通道A出口的方向是渐缩的，以实现向细长通道的过渡。如图2和图3中所示的结构，与第二圆柱部对应的第一材料通道A是截面积较大的柱形通道，通过第二圆锥部的过渡作用，实现了与第二引导部21对应的第一材料通道A为细长的柱形通道。而且细长通道最好能供单列的主材(例如细胞)通过，以引导主材更顺畅地喷出以被辅材包裹，从而形成满足尺寸要求的生物打印材料。
下面将结合图2所示的实施例，对本发明此种喷头组件的工作原理进行详细说明。在压力的控制下，主材沿着第一材料通道A流动，辅材沿着渐缩的第二材料通道B流动，且具有一定的压力，在达到第二材料通道B的出口时附着到到达第一材料通道A出口的主材上，逐渐实现全部包裹形成流体打印单元，最终从喷头组件的出口有序地喷出。
另外，本发明还提供了一种生物打印机，如图4和图5所示的实施例，其包括上述的生物打印机喷头组件，由于本发明生物打印机喷头组件能够在打印时对细胞进行包裹，从而对细胞进行保护而不受损伤，具有较高的存活率，因而采用此种喷头组件的生物打印机也具备相应的有益技术效果，能够通过获得优质的生物打印材料以使制作的各种生物构建体能够保持较好的生物学性能，例如较高的活性和较长的寿命等。优选地，生物打印机为3D生物打印机， 本发明生物打印机喷头组件尤其适用于3D生物打印机。
在本发明的一个实施例中，该生物打印机还包括固定板7、安装支座8、第一材料供应源31和第二材料供应源32，固定板7作为整个生物打印机中各部件的基准，安装支座8设在固定板7一侧中下部的位置，第一材料供应源31和第二材料供应源32分别用于容纳主材和辅材，且左右并排设在安装支座8内，例如，材料供应源可以是容纳材料的容器。本发明的喷嘴组件P(参见图4)安装在第一材料供应源31正下方，以使第一材料供应源31与第一材料通道A连通，第二材料供应源32与第二材料通道B通过连接管3连通。
对于该实施例的生物打印机，需要通过压力控制来实现生物打印材料的流动，优选为气压控制，因而生物打印机还需包括压力调节装置，例如选择电气比例阀等。当第一材料供应源31和第二材料供应源32中装好材料后，分别与气路中的电气比例阀连接，两个材料供应源各自对应的电气比例阀再与控制系统进行实时的模拟量通信，经上位软件发出指令分别对两个材料供应源的挤出量进行控制。通过分别调节气压，可控制第一材料通道A和第二材料通道B内流体的喷出速度。
根据不同的打印需求，可以采取不同的控制方式：
当需要形成连续的生物打印材料时，首先，在第一材料供应源31内添加主材，主材是含有细胞的混合物质，其状态可以是具有流动性的流体，亦可是凝胶状态；在第二材料供应源32内添加辅材，辅材可以是与主材相同的物质(主辅同材)，根据使用需求，辅材可以是供给营养的物质，也可以是提供粘合力的物质(可兼用于形成保护层)，也可以是包覆于主材周围形成保护层的物质。接着，通过压力调节装置向第一材料供应源31内施加恒定的气压，就可以使主材持续喷出，外面通过辅材进行包裹，整体成细长状结构。
当需要形成颗粒状的生物打印材料时，可以在第一材料供应源31内添加主材，在第二材料供应源32内添加辅材，并对第一材料 供应源31频繁地加压与开闭形成脉冲，用于控制主材从第一材料通道A的出口以离散颗粒形式喷出；并向第二材料供应源32内施加连续的压力，用于控制辅材从第二材料通道B的出口连续喷出，当然也可以向第二材料供应源32内施加脉冲压力。参见图3，通过对主材的脉冲控制，就可以形成离散的颗粒，并与外层辅材结合，形成类似蛋黄蛋清的流体打印单元，并在温度的作用下迅速冷却定型。
作为更优选的实施例，本发明的生物打印机还包括用于控制主材和辅材的温度的温控装置9，第一材料供应源31和第二材料供应源32可以采用独立的温控装置9。参见图5，温控装置9设在安装支座8上远离固定板7的一侧，温控装置9从靠近安装支座8的一端起依次包括：制冷片91、散热器92和散热风扇93，制冷片91靠近安装支座8的表面设置，可以在外部温度控制器的控制下对生物打印材料进行加热或冷却，制冷片91可以选择半导体制冷片，半导体制冷片的控温端与材料供应源相对设置，非控温端与散热器92相对设置，散热器92用于带走半导体制冷片在工作时产生的热量，散热风扇93用于加速散热器92的散热进程。更进一步地，还可以在第一材料供应源31和第二材料供应源32的外部设置温度检测装置，以对主材和辅材的当前温度进行更加准确的判断。
该实施例中通过设置温控装置9，有利于在打印过程中保持生物材料的活性，尤其是采用高黏度的生物打印材料时，能够提高流动性，尽量避免由于温度较低而导致喷嘴堵塞的现象，从而提高打印效率。
在另一个更优选的实施例中，生物打印机还包括位置检测装置13，位置检测装置13设在安装于固定板7上的滑槽33中，位置检测装置13可沿滑槽33上下移动，用于在打印前对打印平台的高度进行判断，以确定喷头组件的初始打印位置，从而获得较高的打印精度。
以上对本发明所提供的一种生物打印机喷头组件及生物打印 机进行了详细介绍。本文中应用了具体的实施例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想。应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以对本发明进行若干改进和修饰，这些改进和修饰也落入本发明权利要求的保护范围内。

Claims (11)

  1. 一种生物打印机喷头组件，其特征在于，包括具有第二通道的外喷嘴(1)和具有第一材料通道(A)的内喷嘴(2)，所述内喷嘴(2)同轴设在所述第二通道内，所述外喷嘴(1)和所述内喷嘴(2)之间的环形空间形成第二材料通道(B)，所述第二材料通道(B)在所述第一材料通道(A)的出口处环绕着所述第一材料通道(A)，用于使从所述第二材料通道(B)出口喷出的第二材料朝向从所述第一材料通道(A)出口喷出的第一材料汇聚，以形成流体打印单元。
  2. 根据权利要求1所述的生物打印机喷头组件，其特征在于，还包括第一材料供应管(4)和外喷嘴固定部(5)，所述外喷嘴(1)可拆卸地连接至所述第一外喷嘴固定部(5)，所述内喷嘴(2)可拆卸地连接至所述第一材料供应管(4)。
  3. 根据权利要求2所述的生物打印机喷头组件，其特征在于，包括由多个不同规格的喷嘴组成的喷嘴套件组，所述外喷嘴(1)和所述内喷嘴(2)从所述喷嘴套件组中选出。
  4. 根据权利要求1所述的生物打印机喷头组件，其特征在于，在所述第二材料通道(B)的出口处，所述外喷嘴(1)的开口低于所述内喷嘴(2)的开口。
  5. 根据权利要求1所述的生物打印机喷头组件，其特征在于，所述外喷嘴(1)包括第一引导部(11)，所述内喷嘴(2)包括第二引导部(21)，所述第一引导部(11)和所述第二引导部(21)邻近于所述喷头的出口设置，所述第一引导部(11)和所述第二引导部(21)之间的第一环形空间沿着朝向所述第二材料通道(B)出口的方向是渐缩的。
  6. 根据权利要求5所述的生物打印机喷头组件，其特征在于，所述外喷嘴(1)还包括与所述第一引导部(11)连接的第一主体部(12)，所述内喷嘴(2)还包括与所述第二引导部(21)连接 的第二主体部(22)，所述第一主体部(12)和所述第二主体部(22)之间的第二环形空间至少部分朝向所述第二材料通道(B)出口的方向是渐缩的。
  7. 根据权利要求6所述的生物打印机喷头组件，其特征在于，所述第一材料通道(A)在所述第一环形空间中的部分为细长的，所述第一材料通道(A)在所述第二环形空间中的部分至少局部朝向所述第一材料通道(A)出口的方向是渐缩的。
  8. 一种生物打印机，其特征在于，包括权利要求1所述的生物打印机喷头组件。
  9. 根据权利要求8所述的生物打印机，其特征在于，还包括压力调节装置，所述压力调节装置用于控制所述第一材料通道(A)和所述第二材料通道(B)内的流体压力。
  10. 根据权利要求8所述的生物打印机，其特征在于，还包括用于控制所述第一材料通道(A)和所述第二材料通道(B)的温度的温控装置(9)。
  11. 根据权利要求8所述的生物打印机，其特征在于，还包括：用于对所述喷头组件的初始打印位置进行判断的位置检测装置(13)。
PCT/CN2015/099822 2015-12-30 2015-12-30 生物打印机喷头组件及生物打印机 WO2017113167A1 (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2018534155A JP6820933B2 (ja) 2015-12-30 2015-12-30 バイオプリンタースプレーヘッドアセンブリ及びバイオプリンター
EP15911796.9A EP3398777B1 (en) 2015-12-30 2015-12-30 Bioprinter nozzle assembly and bioprinter
US16/067,541 US11161299B2 (en) 2015-12-30 2015-12-30 Bioprinter spray head assembly and bioprinter
PCT/CN2015/099822 WO2017113167A1 (zh) 2015-12-30 2015-12-30 生物打印机喷头组件及生物打印机

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/099822 WO2017113167A1 (zh) 2015-12-30 2015-12-30 生物打印机喷头组件及生物打印机

Publications (1)

Publication Number Publication Date
WO2017113167A1 true WO2017113167A1 (zh) 2017-07-06

Family

ID=59224240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/099822 WO2017113167A1 (zh) 2015-12-30 2015-12-30 生物打印机喷头组件及生物打印机

Country Status (4)

Country Link
US (1) US11161299B2 (zh)
EP (1) EP3398777B1 (zh)
JP (1) JP6820933B2 (zh)
WO (1) WO2017113167A1 (zh)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108654870A (zh) * 2018-06-21 2018-10-16 南京林业大学 一种外混式双流体扇形雾喷头
CN108839343A (zh) * 2018-08-02 2018-11-20 中国科学技术大学 生物光学仿体的中空微管道的制备装置和制备方法
CN110257243A (zh) * 2019-07-23 2019-09-20 清华大学深圳研究生院 微流控芯片打印喷嘴和生物3d打印系统
KR102065474B1 (ko) * 2018-10-10 2020-01-14 재단법인 의약바이오컨버젼스연구단 3d 바이오프린터 및 이의 운용 방법
CN110870940A (zh) * 2018-09-04 2020-03-10 上海叁钛生物科技有限公司 皮肤原位打印用打印头装置
CN110870938A (zh) * 2018-09-04 2020-03-10 上海叁钛生物科技有限公司 皮肤原位打印用打印头装置
CN111957508A (zh) * 2020-07-06 2020-11-20 厦门鑫奥格自动化设备有限公司 点胶机用大滴液点胶针
WO2021136297A1 (zh) * 2019-12-30 2021-07-08 四川蓝光英诺生物科技股份有限公司 生物打印机喷头、生物打印机和管腔组织构建体打印方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11472102B2 (en) * 2016-12-08 2022-10-18 President And Fellows Of Harvard College Core-shell nozzle for three-dimensional printing and method of use
CN113211784B (zh) * 2020-02-05 2022-02-18 中国科学院福建物质结构研究所 一种具有高隔音性能的制件及其基于3d打印的制备方法
CN111730864A (zh) * 2020-07-23 2020-10-02 宁波大学 一种用于生物3d打印的同轴喷头
CN112373026B (zh) * 2020-10-26 2022-09-16 华东数字医学工程研究院 变径管打印喷头及变径管打印方法
CN113172887B (zh) * 2021-03-15 2023-04-07 中国科学院上海硅酸盐研究所 一种具有缓释功能的同轴支架材料
CN113400435A (zh) * 2021-06-18 2021-09-17 河北工业大学 3d打印双混凝土协同挤出系统
CN113290858B (zh) * 2021-07-19 2024-06-11 中国医科大学 可拆卸挤出式生物3d打印同轴喷头
CN113478825A (zh) * 2021-07-20 2021-10-08 杭州捷诺飞生物科技股份有限公司 复合挤出式3d打印机
CN113650295A (zh) * 2021-08-12 2021-11-16 河北化工医药职业技术学院 3d打印机用喷头
WO2023081955A1 (en) * 2021-11-09 2023-05-19 Padstone Pty Ltd Bacteria application device and methods of use
SE2250804A1 (en) * 2022-06-29 2023-12-30 Cellink Bioprinting Ab Nozzle for coaxial extrusion

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103911678A (zh) * 2014-04-17 2014-07-09 华中科技大学 一种用于电流体喷印的同轴喷嘴
CN104873300A (zh) * 2015-05-11 2015-09-02 浙江大学 一种具有内置营养通道的三维生物结构的打印装置
CN204839829U (zh) * 2015-05-07 2015-12-09 周惠兴 一种具有自动包裹功能的喷头装置及包裹系统
CN105167879A (zh) * 2015-06-25 2015-12-23 南京律动生物科技有限公司 一种双流道喷嘴

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2764486Y (zh) 2005-03-11 2006-03-15 中国人民解放军第二军医大学 超临界流体制备微粒的可组合式雾化喷头
US9126366B2 (en) * 2011-06-15 2015-09-08 Korea Institute Of Machinery & Materials Apparatus and method for manufacturing cell culture scaffold
CN104441654B (zh) 2014-10-27 2016-08-24 清华大学深圳研究生院 一种三维生物打印装置及方法
CN204722376U (zh) 2015-05-05 2015-10-28 天津卿壹创想科技有限公司 一种精确导向的3d打印机

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103911678A (zh) * 2014-04-17 2014-07-09 华中科技大学 一种用于电流体喷印的同轴喷嘴
CN204839829U (zh) * 2015-05-07 2015-12-09 周惠兴 一种具有自动包裹功能的喷头装置及包裹系统
CN104873300A (zh) * 2015-05-11 2015-09-02 浙江大学 一种具有内置营养通道的三维生物结构的打印装置
CN105167879A (zh) * 2015-06-25 2015-12-23 南京律动生物科技有限公司 一种双流道喷嘴

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP3398777A4 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108654870A (zh) * 2018-06-21 2018-10-16 南京林业大学 一种外混式双流体扇形雾喷头
CN108839343A (zh) * 2018-08-02 2018-11-20 中国科学技术大学 生物光学仿体的中空微管道的制备装置和制备方法
CN108839343B (zh) * 2018-08-02 2024-01-05 中国科学技术大学 生物光学仿体的中空微管道的制备装置和制备方法
CN110870940A (zh) * 2018-09-04 2020-03-10 上海叁钛生物科技有限公司 皮肤原位打印用打印头装置
CN110870938A (zh) * 2018-09-04 2020-03-10 上海叁钛生物科技有限公司 皮肤原位打印用打印头装置
KR102065474B1 (ko) * 2018-10-10 2020-01-14 재단법인 의약바이오컨버젼스연구단 3d 바이오프린터 및 이의 운용 방법
WO2020076094A1 (ko) * 2018-10-10 2020-04-16 재단법인 의약바이오컨버젼스연구단 3d 바이오프린터 및 이의 운용 방법
CN110257243A (zh) * 2019-07-23 2019-09-20 清华大学深圳研究生院 微流控芯片打印喷嘴和生物3d打印系统
CN110257243B (zh) * 2019-07-23 2021-06-25 清华大学深圳研究生院 微流控芯片打印喷嘴和生物3d打印系统
WO2021136297A1 (zh) * 2019-12-30 2021-07-08 四川蓝光英诺生物科技股份有限公司 生物打印机喷头、生物打印机和管腔组织构建体打印方法
CN111957508A (zh) * 2020-07-06 2020-11-20 厦门鑫奥格自动化设备有限公司 点胶机用大滴液点胶针

Also Published As

Publication number Publication date
US11161299B2 (en) 2021-11-02
JP6820933B2 (ja) 2021-01-27
EP3398777B1 (en) 2021-09-08
EP3398777A1 (en) 2018-11-07
JP2019504620A (ja) 2019-02-21
US20190255770A1 (en) 2019-08-22
EP3398777A4 (en) 2019-09-11

Similar Documents

Publication Publication Date Title
WO2017113167A1 (zh) 生物打印机喷头组件及生物打印机
CN105647801B (zh) 生物打印机喷头组件及生物打印机
JP2019503681A (ja) バイオプリンタースプレーヘッドアセンブリ及びバイオプリンター
CN105050731B (zh) 计量阀和计量方法
WO2005009627A3 (en) Apparatus and method for dispensing discrete amounts of viscous material
JPH05212335A (ja) 粘性液体の多数のビードを吐出する方法と装置
JP7482857B2 (ja) 投与材料冷却装置を備える投与システム
CN205251765U (zh) 生物打印机
WO2017109964A1 (ja) ３次元積層造形装置、３次元積層造形装置の制御方法および３次元積層造形装置の制御プログラム
WO2017113186A1 (zh) 生物打印机喷头组件及生物打印机
KR101866516B1 (ko) 부위별 분사량이 조절되는 이형제 스프레이 장치
KR101930157B1 (ko) 점성 용액 도포 장치 및 점성 용액 도포 방법
CN208250418U (zh) 一种针式同轴送粉头
CN105647804B (zh) 生物打印机喷头组件及生物打印机
EP1790923B1 (en) Spray head
CN105661607B (zh) 一种远端供料的食品3d打印装置
CN201841379U (zh) 一种密封式焊头的气体保护装置
TWM628627U (zh) 塗佈裝置的流體控制結構
CN206344455U (zh) 一种3d打印装置及系统
CN220546921U (zh) 滴球成型装置和油氨柱成型装置
CN216965159U (zh) 一种加长型压电喷射阀用点胶喷嘴
JP2010172795A (ja) コーティング塗布治具及びコーティング塗布装置
CN207929163U (zh) 一种胶丸切割装置和包括其的胶丸制作系统
CN204891809U (zh) 一种膨化冷却塔排料口
US20150076179A1 (en) Dispenser for dispensing a fluent material

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15911796

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2018534155

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2015911796

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2015911796

Country of ref document: EP

Effective date: 20180730