WO2017090970A1 - Composition destinée à protéger la peau contre les rayons ultraviolets, qui contient un polysaccharide extracellulaire produit par ceriporia lacerata comme principe actif - Google Patents

Composition destinée à protéger la peau contre les rayons ultraviolets, qui contient un polysaccharide extracellulaire produit par ceriporia lacerata comme principe actif Download PDF

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WO2017090970A1
WO2017090970A1 PCT/KR2016/013531 KR2016013531W WO2017090970A1 WO 2017090970 A1 WO2017090970 A1 WO 2017090970A1 KR 2016013531 W KR2016013531 W KR 2016013531W WO 2017090970 A1 WO2017090970 A1 WO 2017090970A1
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culture medium
composition
extracellular polysaccharide
skin
mycelium
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PCT/KR2016/013531
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English (en)
Korean (ko)
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김윤수
유혜동
신은지
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㈜퓨젠바이오
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention is an active ingredient produced by Ceriporia lacerate ( Ceriporia lacerate ); Mycelium culture medium of Ceriporia laccerata containing the active ingredient; Dry powder of the mycelia culture medium; Or it relates to a composition for protecting the skin against ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • Ultraviolet rays are light rays having a wavelength of 400 nm or less emitted from the sun and damage the skin. Ultraviolet rays are divided into long-wave ultraviolet (UV-A; 320-400 nm), medium-wave ultraviolet (UV-B; 280-320 nm), and short-wave ultraviolet (UV-C; 200-250 nm). It causes sunburn, pigmentation, photoaging, skin cancer, and various skin damages depending on the wavelength.
  • UV-A long-wave ultraviolet
  • UV-B medium-wave ultraviolet
  • UV-C short-wave ultraviolet
  • Short-wave ultraviolet rays cause bactericidal action and cell death, but most of them are absorbed by the ozone layer, making it difficult to reach the skin.
  • Medium-wave ultraviolet rays reach the epidermal base layer and the upper dermis to sustain skin keratinization and cause sunburn and pigmentation of erythema and blisters. It not only causes cell membrane damage and skin cancer, but also causes sunburn (sunburn). Furthermore, it directly damages the DNA of skin cells and directly affects skin regeneration mechanisms.
  • long-wave UV rays can reach the dermis and cause immediate photodeposition, natural erythema, phototoxicity, photoallergic reactions, and chronic photoaging due to free radical generation, and degeneration of the elastic and collagen fibers of the dermis Reduce wrinkles and cause wrinkles and skin atrophy. It also affects the DNA of skin cells indirectly, increasing the risk of skin cancer.
  • Korean Patent No. 10-1549148 discloses a composition for protecting and treating skin cells by ultraviolet rays, including a natural extract.
  • Ceriporia laccerata is a type of white fungus, and is known to perform co-metabolism called lignin decomposition in order to use carbon sources such as cellulose, hemicellulose, other polysaccharides, and glycerol in an ecosystem.
  • Ceriporia laccerata Although only the use of Ceriporia laccerata culture extract in the Republic of Korea Patent No. 10-1031605 filed by the present inventors is known. The use of liporia laccerata for protecting skin against ultraviolet light has not been reported.
  • the present inventors are extracellular polysaccharides isolated from the seriporia lacserata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Alternatively, the present invention was completed by confirming that the extract of the mycelium culture medium exhibits a skin protective effect against ultraviolet rays.
  • An object of the present invention is to provide a composition for protecting the skin against ultraviolet rays containing the active ingredient produced by Ceriporia laccerata, and a pharmaceutical composition, cosmetic composition or health functional food comprising the same.
  • Another object of the present invention is to provide a skin protection method using the active ingredient produced by Ceriporia laccerata.
  • Another object of the present invention is to provide a use of the active ingredient produced by Ceriporia laccerata for use in the preparation of a skin protective composition.
  • the present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ); Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a skin protection composition containing the extract of the mycelia culture medium as an active ingredient.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a pharmaceutical composition for treating skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a cosmetic composition for preventing or improving skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a health functional food for preventing or improving skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a skin protection method comprising administering the extract of the mycelium culture to a subject in need thereof.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata for use in the preparation of a composition for skin protection; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a use of the extract of the mycelium culture.
  • Extracellular polysaccharide produced by the seriporia laccerata according to the present invention Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium;
  • the composition for protecting the skin against ultraviolet rays containing the extract of the mycelium culture medium as an active ingredient has a protective effect on skin keratinocytes exposed to ultraviolet rays, and thus prevents, improves or treats skin damage caused by ultraviolet rays, a cosmetic composition or a health composition. It can be usefully used as a nutraceutical.
  • HaCaT human keratinocytes
  • EPS Ceriporia laccerata extracellular polysaccharide
  • the present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ); Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a skin protection composition containing the extract of the mycelia culture medium as an active ingredient.
  • extracellular polysaccharide is a part of the cell wall of a microorganism such as a fungus, the polysaccharide is secreted to the outside of the cell to form a capillary around it, or as a slime to substances that are secreted around the cell or medium it means.
  • the extracellular polysaccharide is secreted by the microorganism to protect itself from the external environment such as antibodies, toxic substances, protozoa and bacteriophage.
  • the extracellular polysaccharide is 40 to 60% by weight sugar and 30 to 40% by weight protein, 40 to 50% by weight sugar and 32 to 38% by weight protein, 43 to 47% by weight sugar and 33 to 36% by weight Protein, or about 45% by weight sugar and about 34% by weight protein.
  • the sugar may contain mannose, galactose and glucose.
  • the extracellular polysaccharide may have a molecular weight of 100 to 150 kDa, 110 to 140 kDa or 115 to 125 kDa, and more specifically may have a molecular weight of about 120 kDa.
  • skin protection refers to protecting the skin from external irritation or skin damage caused by the environment, and may also include skin changes due to aging.
  • One of the external stimuli that can damage the skin is ultraviolet rays, and the skin damage caused by ultraviolet rays may include sunburn, pigmentation, photoaging, natural erythema, decreased elasticity, wrinkles, skin cancer, and the like.
  • the extracellular polysaccharide comprises the steps of: (a) liquid culture of the Ceriporia laccerata mycelium to prepare a Ceriporia laccerata mycelium culture solution; (b) drying and powdering the Ceriporia laccerata mycelium culture solution; And (c) extracting the Ceriporia laccerata mycelium culture powder with a solvent, filtering the same, and concentrating under reduced pressure.
  • the medium for the liquid culture of the seriporia laccerata mycelium in the step (a) is sugar, glucose, starch, hydrate, soybean meal, soybean meal, magnesium sulfate (MgSO 4 ), potassium phosphate (KH 2 PO 4 ), potassium diphosphate (K 2 HPO 4 ) and water, and the hydrogen ion concentration (pH) may be 4.5 to 6.0.
  • the medium is 0.2 to 3% by weight of sugar, 0.2 to 3% by weight of glucose, 0.2 to 4% by weight of starch, 0.1 to 0.5% by weight of water, 0.1 to 0.5% by weight of wheat flour, 0.2 to 3% of soy flour %, 0.05 to 0.1 wt% magnesium sulfate (MgSO 4 ), 0.05 to 0.25 wt% potassium monophosphate (KH 2 PO 4 ), 0.05 to 0.25 wt% potassium phosphate (K 2 HPO 4 ) and the balance of water Can be.
  • MgSO 4 magnesium sulfate
  • KH 2 PO 4 potassium monophosphate
  • K 2 HPO 4 potassium phosphate
  • the liquid culture in step (a) may be performed under a blue LED light source, and may be performed by maintaining the concentration of carbon dioxide at 1,000 to 2,000 ppm.
  • the liquid culture for example, at 20 to 28 °C, hydrogen ion concentration of 4.5 to 6.0, light source to maintain a blue LED, illuminance of 0.1 to 0.8 LUX and air is injected at 0.5 to 2.0 kgf / cm2,
  • the concentration may be performed for 8 to 13 days while maintaining at 1,000 to 2,000 ppm. Specifically, it may be performed for 5 to 15 days at the conditions of 20 to 25 °C, pH 4.5 to 6.0, air injection of 0.5 to 2.0 kgf / cm2 and carbon dioxide concentration of 1,000 to 2,000 ppm.
  • the content of the extracellular polysaccharide is high, which is preferable.
  • the parent strain of step (a) maintains a constant temperature of 25 °C in a shaker incubator using a PDB (potato dextrose broth) medium in a conical flask for the excellent strain stored in 1 to 5 °C in the PDA (potato dextrose agar) medium state Can be used after 7 to 9 days incubation process.
  • the culture solution or the obtained mycelium can be used as the inoculum.
  • the amount of mycelia to be added to the inoculum is preferably about 0.5% (w / v) based on the amount of the solution to be cultured.
  • the amount of extracellular polysaccharides does not increase as the amount of mycelia (% / 100 ml, w / v) increases, so the medium composition has the highest content of extracellular polysaccharides, not the best nutritional ratio and environmental conditions for the growth of mycelia. It is preferable to apply the selective culture conditions to form.
  • the culture solution can be separated and purified into a mycelium and an aqueous solution.
  • the separation and purification may be performed by repeatedly purifying a solution obtained by removing the mycelium by a centrifuge with a multi-sheet filter press and a vibration centrifugal separator (PALLSEP), and then irradiating ultraviolet (UV) light for 1 minute. have.
  • the culture solution may be sealed and stored after removing the oxygen, which may bring a change in the content of the active ingredient due to the growth of the mycelia when the mycelium is present in the culture solution.
  • step (b) the mycelia culture solution prepared in step (a) may be dried and powdered.
  • the drying may be performed for 48 to 96 hours at a temperature of 40 °C or less, more specifically 30 °C or less to prevent the disappearance of the active material.
  • the drying of step (b) is preferable to use a vacuum freeze dryer rather than a vacuum dryer that sets the evaporation temperature relatively high because the change in the effective substance content is minimized.
  • step (c) after extracting the dry powder of the mycelia culture solution obtained in step (b) with a solvent, the extracellular polysaccharide which is the active ingredient of the composition according to the present invention is separated.
  • distilled water 100 ml of distilled water is added to 3 to 10 g of the dry powder of the mycelium culture medium, followed by well suspension, followed by centrifugation at 5,000 to 10,000 rpm for 10 to 30 minutes to obtain a supernatant. After adding the extraction solvent corresponding to 3 times, it can put in a refrigerator of 1-5 degreeC, and can be left for 10 to 15 hours. After only centrifuging the supernatant again at 5,000 to 10,000 rpm for 10 to 30 minutes in the stationary material, the precipitate may be recovered to prepare a crude extracellular polysaccharide. The crude extracellular polysaccharide can be vacuum-dried at 30 ° C. or lower to obtain extracellular polysaccharide.
  • the extraction solvent may be a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, acetone, ether, chloroform and ethyl acetate or a mixed solvent thereof, and more specifically, water, methanol, ethanol, butanol , Acetone and ethyl acetate may be a solvent selected from the group consisting of, or a mixed solvent thereof, more preferably, water or 50 to 99% (v / v) of ethanol aqueous solution.
  • the skin protection composition may be to protect the skin from damage by ultraviolet rays.
  • composition for protecting skin according to the present invention may be used as an additive in pharmaceutical compositions, cosmetic compositions or health functional foods for the purpose of preventing, improving or treating skin damage to ultraviolet rays, wherein the amount of use and the form of use are appropriately adjusted according to the purpose of manufacture.
  • Skin damage caused by ultraviolet rays may include sunburn, pigmentation, photoaging, natural erythema, decreased elasticity, wrinkles or skin cancer.
  • the extracellular polysaccharide may be included in 0.1 to 80% by weight, or 0.1 to 50% by weight relative to the total weight of the skin protective composition, the mycelium culture medium, the dry powder thereof or the extract of the mycelium culture medium of Seriphoria laccerata It may be appropriately included in an amount corresponding to the content of the external polysaccharide. More specifically, however, extracellular polysaccharides; Culture solution comprising the same; Dry powder of the culture solution; Alternatively, the effective amount of the extract of the culture solution may be appropriately adjusted according to the method of use and purpose of the skin protection composition.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a pharmaceutical composition for treating skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the extracellular polysaccharide, mycelium culture medium of the three liporia laccerata containing the same, its dry powder or extract is as described above.
  • the pharmaceutical composition is an extracellular polysaccharide produced by Seriporia laccerata; Mycelium culture medium of Seriporia laccerata comprising the same; Dry powder of the mycelia culture medium; Or in addition to containing the extract of the mycelium culture medium as an active ingredient, suitable carriers, excipients and diluents commonly used may be further included.
  • compositions according to the invention can each be formulated and used according to conventional methods. Suitable formulations include, but are not limited to, tablets, pills, powders, granules, dragees, hard or soft capsules, solutions, suspensions or emulsions, injections, suppositories, and the like.
  • compositions according to the invention can be prepared in suitable formulations using pharmaceutically inert organic or inorganic carriers. That is, when the formulation is a tablet, coated tablet, dragee and hard capsule, it may include lactose, sucrose, starch or derivatives thereof, talc, calcium carbonate, gelatin, stearic acid or salts thereof. In addition, when the formulation is a soft capsule, it may include polyols of vegetable oils, waxes, fats, semisolids and liquids. Furthermore, when the formulation is in the form of a solution or syrup, it may include water, polyols, glycerol, vegetable oils and the like.
  • the pharmaceutical composition according to the present invention may further include a preservative, a stabilizer, a humectant, an emulsifier, a solubilizer, a sweetener, a colorant, an osmotic agent, an antioxidant, and the like, in addition to the carrier.
  • the method of administering the pharmaceutical composition according to the present invention can be easily selected according to the dosage form, and can be administered orally or parenterally.
  • the dosage may vary depending on the age, sex, weight, severity and path of administration of the patient, but is generally 5 to 1,000 mg / kg body weight based on the extracellular polysaccharide as an active ingredient, specifically 10 to 600
  • the amount of mg / kg body weight may be administered by dividing once to three times a day.
  • the above dosage does not limit the scope of the present invention.
  • the pharmaceutical composition according to the present invention not only provides an excellent skin protection effect against ultraviolet rays, and has almost no toxicity and side effects caused by drugs, so that the pharmaceutical composition can be used safely for long-term use for the purpose of treating skin damage caused by ultraviolet rays.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a cosmetic composition for preventing or improving skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the extracellular polysaccharide, mycelium culture medium of the three liporia laccerata containing the same, its dry powder or extract is as described above.
  • the cosmetic composition may be directly applied to the skin for the purpose of preventing or improving skin damage caused by ultraviolet rays.
  • the cosmetic composition may be formulated into a cosmetic formulation prepared conventionally.
  • the cosmetic composition may include solutions, external ointments, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, foundations, sprays, and the like.
  • the cosmetic composition may be in the form of a lotion, nutrition cream, massage cream, essence, eye cream, cleansing foam, cleansing water, pack, spray or powder.
  • the cosmetic composition may further include a carrier that can be blended in general skin cosmetics.
  • the carrier may be, for example, an oil component, water, a surfactant, a humectant, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative, a perfume, and the like, but is not limited thereto.
  • the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide and mixtures thereof
  • Carrier components selected from the group may be included.
  • the formulation of the cosmetic composition when the formulation of the cosmetic composition is a powder or a spray, it may include a carrier component selected from the group consisting of lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, and mixtures thereof.
  • a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether.
  • the formulation of the cosmetic composition may include a carrier component selected from the group consisting of solvents, solvating agents, emulsions and mixtures thereof.
  • the solvent, solvating agent or emulsifying agent may be water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, Sorbitan fatty acid esters, mixtures thereof, and the like.
  • the formulation of the cosmetic composition is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol
  • Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters
  • a carrier component selected from the group consisting of microcrystalline cellulose, aluminum metahydroxy, bentonite, agar, tragacanth and mixtures thereof.
  • the cosmetic composition may further include an adjuvant selected from the group consisting of antioxidants, stabilizers, solubilizers, humectants, pigments, flavors, sunscreens, colorants, surfactants, and combinations thereof.
  • an adjuvant selected from the group consisting of antioxidants, stabilizers, solubilizers, humectants, pigments, flavors, sunscreens, colorants, surfactants, and combinations thereof.
  • the adjuvant is not limited to use as long as the adjuvant commonly used in the preparation of the cosmetic composition.
  • the present invention is an extracellular polysaccharide produced by Ceriporia laccerata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a health functional food for preventing or improving skin damage by ultraviolet rays containing the extract of the mycelia culture medium as an active ingredient.
  • the extracellular polysaccharide, mycelium culture medium of the three liporia laccerata containing the same, its dry powder or extract is as described above.
  • the health functional food may be in the form of powder, granules, tablets, capsules or beverages.
  • the health functional food may be candy, chocolate, gum, tea, vitamin complex, health supplement food and the like.
  • the content of the extract of the mycelium culture may be generally included in 0.01 to 50% by weight, or 0.1 to 20% by weight of the total food weight.
  • the health functional food is an extracellular polysaccharide of the seriporia lacserata of the present invention; Mycelium culture medium containing this; Dry powder of the mycelia culture medium; Or it may further include a food supplement acceptable food additive with the extract of the mycelia culture medium.
  • the present invention is an extracellular polysaccharide produced by the seriporia lacserata; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a skin protection method comprising administering the extract of the mycelium culture to a subject in need thereof.
  • the extracellular polysaccharide, mycelium culture medium of the three liporia laccerata containing the same, its dry powder or extract is as described above.
  • the subject of the skin protection method may be a mammal, in particular a human.
  • the present invention also provides an extracellular polysaccharide produced by Ceriporia laccerata for use in the preparation of a composition for protecting skin; Mycelium culture medium of Seriporia laccerata containing the extracellular polysaccharide; Dry powder of the mycelia culture medium; Or it provides a use of the extract of the mycelium culture.
  • the extracellular polysaccharide, mycelium culture medium of the three liporia laccerata containing the same, its dry powder or extract is as described above.
  • PDA potato dextrose agar
  • PDA culture strain 4 ° C. refrigerator
  • the dry powder of the seriporia laccerata mycelium culture medium was prepared by pulverizing the lyophoric acid culture medium prepared in Preparation Example 1 by vacuum freeze drying at 25 ° C. for 72 hours using a vacuum freeze dryer. .
  • the extract of the seriporia laccerata mycelium culture obtained in Preparation Example 3 was further centrifuged at 8,000 rpm for 20 minutes. Thereafter, the precipitate was recovered to obtain a crude EPS.
  • the crude EPS was vacuum-dried at 25 ° C. for 72 hours using a vacuum freeze dryer to obtain EPS produced by Ceriporia laccerata.
  • the EPS prepared in Preparation Example 4 was dissolved in 0.1 M Na 2 SO 4 /0.05 M NaN 3 [adjusting pH to 4 with glacial acetic acid] to 1% (w / v) in a solution, and then 4,000 rpm. After centrifugation for 0.5 hours, only the supernatant was filtered with a 0.45 ⁇ m syringe filter and analyzed by GPC.
  • a refractive index was used as a detector
  • a GPC column was used with OHpak SB 805 HQ (Shodex, Japan)
  • the mobile phase was 0.1 M Na 2 SO 4 /0.05 M NaN 3 [glacial acetic acid]. Adjust the pH to 4], and the flow rate of the mobile phase was flowed at a rate of 0.1 mL / min.
  • Standard curves were prepared using dextran (American Polymer Corporation, USA) with different molecular weights (130 kDa, 400 kDa, 770 kDa or 1,200 kDa).
  • the molecular weight of EPS was measured using a refractive index (RI) measuring device Knauer K-2310 (Germany). Measurement conditions are shown in Table 1 below.
  • the EPS molecular weight of the present invention was found to be about 120 kDa.
  • the EPS prepared in Preparation Example 4 was second purified, and proteolytic enzyme treatment was performed to determine sugar and protein content.
  • the super purified EPS (EPS prepared in Preparation Example 4) was dissolved in distilled water and centrifuged at 8,000 rpm for 20 minutes to separate the supernatant. Ethanol corresponding to 2-3 times the amount was added to the separated supernatant and placed in a 4 ° C. refrigerator for 12 hours. Thereafter, only the supernatant was taken from the stationary material, which was again centrifuged at 8,000 rpm for 20 minutes, and the precipitate was recovered to obtain a second purified EPS.
  • the second purified EPS was dissolved in distilled water, and the proteolytic enzyme alcalase was treated at 50 ° C. for 30 minutes at a concentration of 0.5% (w / v).
  • Sugar content was measured by the phenol-sulfuric acid method. Specifically, 25 ⁇ l of 80% (w / v) phenol was added to 1 mL of the sample diluted by concentration, and then 2.5 mL of sulfuric acid was added and cooled to room temperature. This was measured by absorbance at a wavelength of 465 nm to calculate the sugar content.
  • the protein content was measured by the BCA method (Smith PK et al., Analytical Biochemistry, 150 (1): 76-85, 1985), and bovine serum albumin was used as a standard.
  • the sugar and protein content measured as described above is shown in Table 2 below, the sugar content of EPS was 45 to 51% by weight, the protein content was found to be 33 to 34% by weight.
  • EPS mainly contains mannose, galactose and glucose.
  • the cell survival rate was measured by MTT analysis.
  • the human keratinocyte line HaCaT cell line (CLS, Germany) was incubated under 37 ° C., 5% CO 2 conditions in DMEM medium supplemented with 10% fetal bovine serum, 100 ⁇ g / ml streptomycin and 100 U / ml penicillin.
  • the cultured cells were incubated for 24 hours by dispensing 100 [mu] l each into 96-well plates to 2x10 4 cells / ml.
  • the EPS was added to a concentration of 25 or 50 ⁇ g / ml.
  • eight UV-B lamps were irradiated to the cell line at a distance of 20 cm for 30 seconds to irradiate ultraviolet rays with an intensity of 20 mJ / cm 2.
  • the survival rate of the HaCaT cell line was about 60% when UVB was irradiated, but the cell survival was increased to about 80% when the UVB was irradiated with UVB.
  • the mycelium culture medium of the Seriphoria laccerata containing the EPS, the dry powder of the mycelium culture medium, and the extract of the mycelium culture medium also have the effect of protecting the skin from damage caused by ultraviolet rays. It can be seen.

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Abstract

La présente invention concerne : un polysaccharide extracellulaire produit par Ceriporia lacerata ; un liquide de culture de mycélium de Ceriporia lacerata, le liquide de culture de mycélium comprenant le polysaccharide extracellulaire ; une poudre séchée du liquide de culture de mycélium ; ou une composition destinée à protéger la peau contre les rayons ultraviolets, la composition contenant un extrait du liquide de culture de mycélium en tant que principe actif. La composition présente l'effet de protection des kératinocytes de la peau exposés aux rayons ultraviolets, et, par conséquent, la composition peut être avantageusement utilisée en tant que composition pharmaceutique, composition cosmétique ou aliment fonctionnel de santé pour prévenir, soulager ou traiter les lésions cutanées provoquées par les rayons ultraviolets.
PCT/KR2016/013531 2015-11-26 2016-11-23 Composition destinée à protéger la peau contre les rayons ultraviolets, qui contient un polysaccharide extracellulaire produit par ceriporia lacerata comme principe actif WO2017090970A1 (fr)

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KR1020150166466A KR101682101B1 (ko) 2015-11-26 2015-11-26 세리포리아 락세라타에 의해 생산되는 세포외다당체를 유효성분으로 함유하는 자외선에 대한 피부 보호용 조성물
KR10-2015-0166466 2015-11-26

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KR102150595B1 (ko) * 2017-06-30 2020-09-01 (주)퓨젠바이오 세리포리아 락세라타 배양액을 포함하는 상처 치료용 약학 조성물
KR20200133063A (ko) 2019-05-15 2020-11-26 김병천 세리포리아 라마리투스 균주 배양물을 유효성분으로 포함하는 피부 개선용 화장료 조성물

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JP2005015348A (ja) * 2003-06-24 2005-01-20 Asahi Denka Kogyo Kk 皮膚化粧料
KR20140081982A (ko) * 2012-12-21 2014-07-02 주식회사 뉴메디온 만가닥버섯 자실체 추출물 또는 만가닥버섯 균사체 추출물을 함유하는 피부 외용제 조성물
US20140193454A1 (en) * 2013-01-09 2014-07-10 Byoung Cheon KIM Method from preparing ceriporia lacerata culture extract and pharmaceutical composition for prevention or treatment of diabetes and diabetic complications comprising ceriporia lacerata culture extract as active ingredient
KR101522415B1 (ko) * 2015-02-26 2015-05-21 한상선 세리포리아 락세라타 추출물을 포함하는 화장료 조성물 및 이를 이용한 화장품

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