WO2017038602A1 - Procédé de réduction de mauvaise odeur - Google Patents

Procédé de réduction de mauvaise odeur Download PDF

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Publication number
WO2017038602A1
WO2017038602A1 PCT/JP2016/074748 JP2016074748W WO2017038602A1 WO 2017038602 A1 WO2017038602 A1 WO 2017038602A1 JP 2016074748 W JP2016074748 W JP 2016074748W WO 2017038602 A1 WO2017038602 A1 WO 2017038602A1
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WO
WIPO (PCT)
Prior art keywords
lactic acid
bacteria
acid bacteria
barn
acd
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PCT/JP2016/074748
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English (en)
Japanese (ja)
Inventor
善夫 内藤
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株式会社バイオバランス
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Priority to JP2017537790A priority Critical patent/JP6864293B2/ja
Publication of WO2017038602A1 publication Critical patent/WO2017038602A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the present invention relates to the field of reducing malodor caused by ammonia generated from manure of animals (particularly livestock animals).
  • the present invention relates to the field of reducing malodors by suppressing the growth of microorganisms (especially microorganisms of the genus Proteus) that produce ammonia from animal manure.
  • the genus Proteus is known as the family Enterobacteriaceae that resides in the stool with urease.
  • Ammonia which is a malodorous component in barns, is known to be generated by the reaction of urea and urease in manure, and in order to prevent malodours in the barn, it is possible to prevent volatilization of ammonia by promptly cleaning the barn.
  • Patent Document 1 lactic acid bacteria
  • Patent Document 2 lactic acid bacteria compounding agents
  • Patent Document 3 lactic acid bacteria compounding agents
  • the present inventor has examined lactic acid bacteria having a proteus growth-inhibiting action in order to reduce the generation of ammonia by urease without cleaning the barn.
  • lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD strain) ( NITE BP-02098) was found to effectively inhibit the growth of the genus Proteus.
  • the present inventor sprayed the lactic acid bacteria BB-ACD strain on the flooring material, and the ammonia concentration in the barn decreased. I found out.
  • the present inventor does not spray the lactic acid bacteria BB-ACD strain directly in the barn environment, but ingests the lactic acid bacteria BB-ACD strain in the manure by ingesting it as a feed.
  • the ammonia concentration in the barn also decreased when the animal was ingested as feed.
  • the present inventor has established a method for reducing malodor using lactic acid bacteria BB-ACD strain.
  • the present invention is based on the finding of Lactobacillus acidophilus BB-ACD strain (NITE BP-02098), and specifically relates to the following invention.
  • Lactobacillus acidophilus BB-ACD strain NITE BP-02098).
  • a harmful bacteria growth inhibitor or malodor inhibitor containing the lactic acid bacterium according to (1) (2)
  • the harmful bacteria growth inhibitor or malodor inhibitor according to (2), wherein the harmful bacteria are Escherichia coli and / or Proteus.
  • the harmful fungus growth inhibitor or malodor suppressant according to (2) or (3) which is a preparation for spraying.
  • a livestock feed comprising the lactic acid bacterium according to (1).
  • a method for inhibiting the growth of harmful bacteria comprising spraying the lactic acid bacteria according to (1).
  • a malodor reducing method comprising spraying the lactic acid bacteria according to (1).
  • a malodor reducing method in a barn comprising spraying the lactic acid bacteria described in (1) on a floor in the barn.
  • a malodor reducing method in a barn comprising feeding the lactic acid bacteria described in (1) to a livestock animal.
  • the present invention relates to Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) and a harmful fungus growth inhibitor, a spray preparation, or a livestock feed containing the same.
  • Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) is a lactic acid bacterium obtained by culturing from the small intestine of cattle in Takayama City, Gifu Prefecture, Japan. Deposited under the NITE BP-02098 at the National Institute of Technology Patent Microorganisms Deposit Center (2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture).
  • livestock or livestock animals mean animals raised mainly for the purpose of using their products (milk, meat, eggs, hair, skin, fur, labor force, etc.). Including, horse, buffalo, deer, sheep, goat, alpaca, pig, eagle, rabbit, chicken, eagle, ostrich, turkey, goose, duck, pheasant, guinea fowl.
  • the “harmful fungus” is not particularly limited as long as it is a fungus that exists in manure of livestock animals and adversely affects the growth of livestock. Harmful bacteria include malodor causing bacteria. Examples of harmful bacteria include urease-producing bacteria, preferably bacteria of the genus Escherichia coli and / or Proteus.
  • the “harmful bacteria growth inhibitor” means a drug used for the purpose of inhibiting the growth of the harmful bacteria.
  • the suppression of the growth of harmful bacteria does not mean that it is suppressed by 100%, and it is only required to be suppressed as compared with the drug non-spray group (or non-donating group), for example, 90%, 80% , 70% may be suppressed.
  • Whether or not it is a harmful bacteria growth inhibitor is determined by, for example, spraying the test preparation once a week at 100 g / m 2 on a cow bed, 30 days after the start of the test (such as coliforms, As a control, measure the number of harmful bacteria (eg, coliforms, Proteus, lactic acid bacteria) in fattening cow beds that are not sprayed with lactic acid bacteria, and count the number of harmful bacteria. In comparison, it can be determined that there is an inhibitory effect on the growth of harmful bacteria when there are few harmful bacteria in the cow bed sprayed with the test preparation.
  • harmful bacteria eg, coliforms, Proteus, lactic acid bacteria
  • the harmful fungus growth inhibitor of the present invention may be in a form (spraying preparation) used by being directly sprayed on a livestock barn or the like, or in a form mixed with livestock feed (livestock feed).
  • Formulation for spraying can be in liquid form or for spraying, and livestock feed can be in solid form such as tablet, granule or capsule, gel, liquid etc. according to the feeding method .
  • the fattening cattle barn using wheat straw as the initial covering material was sprayed with 100 g / m 2 of the live lactic acid bacteria prepared in Example 2 once a week, and 30 days after the start of the test, It is a graph showing the result of having measured the number of Proteus genus, lactic acid bacteria.
  • the number of bacteria Cold CFU / g
  • the vertical axis represents the number of bacteria (log CFU / g)
  • the horizontal axis represents bacteria.
  • the graph shows the control on the left and the sprayed area on the right. In the same experiment as FIG.
  • FIG. 3 it is a graph showing the result of having measured the ammonia density
  • the vertical axis represents the ammonia concentration (ppm), and the horizontal axis represents the number of days elapsed since spraying (before spraying, the seventh day of spraying).
  • the lactic acid bacteria viable preparation prepared in Example 2 was sprayed once at 100 g / m 2 on a flat laying house of laying hens. Three days after spraying, the bacteria on the floor of the poultry house (E.
  • the graph shows the control on the left and the sprayed area on the right.
  • FIG. 7 it is a graph showing the result of having measured the ammonia density
  • This medium uses a medium suitable for lactic acid bacteria, the culture temperature is 20 to 45 ° C. (preferably 25 to 40 ° C.), and the culture pH is pH 3.5 to 9.0 (preferably 4. 5 to 7.0), and the culture time can be 6 to 30 hours.
  • the bacterium can be inoculated into an MRS liquid medium and cultured at 30 to 40 ° C. for 24 hours.
  • the culture solution can be sprayed on sterilized bran and cultured at 30 to 40 ° C. for 3 days.
  • the toxic bacteria growth inhibitor or malodor control agent of the present invention can be used as it is in the case of either a spray preparation or a livestock feed. If necessary, an excipient may be added or dispersed in a liquid such as water.
  • the present invention relates to the use of lactic acid bacteria BB-ACD strain for producing harmful bacteria growth inhibitors or malodor inhibitors for use in edible livestock or barns.
  • the present invention also relates to a lactic acid bacterium BB-ACD strain for use in suppressing harmful bacteria growth or reducing malodor in edible livestock or barns.
  • the present invention provides lactic acid bacteria BB-ACD strain (harmful bacteria growth inhibitor, or malodor suppressant, spraying preparation)
  • the present invention relates to a method for inhibiting harmful bacteria growth or a method for reducing malodor.
  • it is desirable that the lactic acid bacteria BB-ACD strain is directly sprayed on the place where the target harmful bacteria or malodor causing bacteria are generated.
  • the spraying is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria, but, for example, once every two weeks or 1-2 times a week. (Preferably once a week), 1.0 ⁇ 10 10 to 1.0 ⁇ 10 12 CFU / m 2 (preferably 3.0 ⁇ 10 11 CFU / m 2 ) .
  • a method for suppressing harmful bacteria growth in a barn and a method for reducing malodor comprising feeding a lactic acid bacteria BB-ACD strain to a livestock animal
  • the present invention provides a lactic acid bacteria BB-ACD strain (a harmful bacteria growth inhibitor,
  • the present invention relates to a method for suppressing the growth of harmful bacteria, or a method for reducing malodor, which comprises feeding livestock animals).
  • the lactic acid bacteria BB-ACD strain is provided to livestock animals together with feed.
  • the lactic acid bacteria BB-ACD strain can be given in solid form such as tablet, granule, capsule form, gel form, liquid form, etc. according to the feeding method as appropriate.
  • the lactic acid bacteria BB-ACD strain is provided as live bacteria.
  • the supply can be determined by the weight of the livestock animal, and is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria. In this case, it can be 10 to 100 g (preferably 40 g) per day.
  • the period of feeding the feed of the present invention to livestock animals is not particularly limited, and can be changed according to the type of livestock animals, but can preferably be fed for the entire period of breeding. However, only part of the breeding period (one year, half year, three months, one month, etc.) may be paid. Except for feeding the feed of the present invention, livestock animals can be raised by commonly used breeding methods.
  • Lactic acid bacteria BB-ACD strain Lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) was obtained by separating and culturing from the small intestine of cattle in Takayama, Gifu, Japan.
  • the lactic acid bacteria BB-ACD strain is registered as NITE BP-02098 (date of trust: August 10th, 2015) with the Patent Microorganism Deposit Center (NPMD), National Institute of Technology and Evaluation (2-5-8, Kazusa-Kamashita, Chiba Prefecture). The day).
  • the bacteriological properties of lactic acid bacteria (Lactobacillus acidophilus BB-ACD) cultured using MRS broth (manufactured by OXOID) are as follows. Regarding sugar assimilation, the basic medium for saccharide fermentability test (tripton 10.0 g, yeast extract 5.0 g, bromcresol purple 0.06 g was added distilled water to a total volume of 1000 ml, and adjusted to pH 6.8. , 121 ° C., high pressure sterilization for 15 minutes).
  • Example 2 Preparation of Lactic Acid Bacteria BB-ACD Strain Bacteria
  • the MRS liquid medium was inoculated with the present bacteria and cultured primarily at 30-40 ° C for 24 hours.
  • the culture solution was sprayed onto a sterilized bran and subjected to secondary culture at 30 to 40 ° C. for 3 days to obtain a lactic acid bacteria viable agent.
  • the number of lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) contained in the obtained lactic acid bacteria viable agent was 3.0 ⁇ 10 9 CFU / g.
  • the lactic acid bacteria BB-ACD strain prepared in this example was used as the lactic acid bacteria.
  • Example 3 Proliferation of Proteus by spraying live lactic acid bacteria and application to ammonia concentration in fattening cattle barn
  • the live lactobacillus prepared in Example 2 was used in a fattening barn using wheat straw as an initial bedding material. It was sprayed at 100 g / m 2 once a week, and the number of cow bed bacteria (E. coli group, Proteus genus, lactic acid bacteria) was measured 30 days after the start of the test. As a control, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the fattening cow bed not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was sprayed was measured using a detector tube before spraying the lactic acid bacterium and before the 30th day of spraying.
  • Example 4 Growth of Proteus by spraying live lactic acid bacteria and the effect on ammonia concentration in fattening piggery Lactic acid bacteria live fungus prepared in Example 2 in fattening piggery using sawdust as an initial covering material was sprayed at 100 g / m 2 once a week, and the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) in the pig bed was measured 7 days after the start of the test. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) in fattening pig beds not sprayed with a lactic acid bacteria viable agent was measured. Moreover, the ammonia concentration in the pig house which sprayed the lactic acid bacteria live bacteria agent was measured using the detection tube twice before the lactic acid bacteria live bacteria drug spraying.
  • the lactic acid bacteria-sprayed pigbed had a higher number of lactic acid bacteria and fewer coliforms and Proteus than the lactic acid bacteria-spattered pigbed. Therefore, it was revealed that the growth of coliforms and Proteus in the pig bed was suppressed by the application of lactic acid bacteria. Moreover, in the pig house after lactic acid bacteria application
  • Example 6 Examination of the effect of Proteus genus growth and ammonia concentration by feeding fattening cattle with a lactic acid bacteria viable agent 40 g of lactic acid bacteria preparation per day is mixed with normal feed to hybrid breeding cattle For 14 days, and the number of bacteria (coliform group, Proteus, lactic acid bacteria) in the cow bed before and after feeding was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the floor that raised fattening cows that were not fed with a lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was fed was measured using a detection tube twice before feeding the lactic bacterium and 14 days after spraying.
  • the lactic acid bacteria BB-ACD strain is not only sprayed, but also when fed, it suppresses the growth of coliforms and Proteus on the barn floor and suppresses the production of ammonia in the barn to reduce malodor Was shown to do.

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Abstract

L'invention fournit une souche de bactérie lactique BB-ACD (souche de Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ BB-ACD) (ＮＩＴＥ ＢＰ－０２０９８) qui a pour action d'inhiber la croissance du Proteus, afin de réduire l'apparition d'un ammoniac sans effectuer de nettoyage d'une étable. La souche de bactérie lactique BB-ACD réduit la concentration en ammoniac à l'intérieur de l'étable par aspersion sur une litière. Enfin, en faisant ingérer la souche de bactérie lactique BB-ACD à des animaux d'élevage en tant qu'aliment, celle-ci est incorporée aux excréments, et il est possible d'inhiber la croissance du Proteus directement dans les excréments.
PCT/JP2016/074748 2015-08-31 2016-08-25 Procédé de réduction de mauvaise odeur WO2017038602A1 (fr)

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JP2017537790A JP6864293B2 (ja) 2015-08-31 2016-08-25 悪臭低減方法

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JP2015-170468 2015-08-31

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108546661A (zh) * 2018-04-26 2018-09-18 湖北华大瑞尔科技有限公司 一种抑菌型微生物发酵剂及其制备方法
CN112335556A (zh) * 2020-10-19 2021-02-09 广州动物园 一种新型圈养野生动物管理方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08298982A (ja) * 1995-05-02 1996-11-19 Aasu Giken:Kk 複合微生物製剤
JPH09252728A (ja) * 1996-03-25 1997-09-30 Natl Fedelation Of Agricult Coop Assoc 消臭性飼料
JP2001299230A (ja) * 2000-04-27 2001-10-30 Korin Korea Co Ltd 飼料添加物およびその製造方法
JP2005065543A (ja) * 2003-08-21 2005-03-17 Oubiken:Kk 家畜の飼育床および家畜の飼育方法
JP2005245299A (ja) * 2004-03-04 2005-09-15 Bio Balance:Kk 新規な乳酸菌
KR100654427B1 (ko) * 2005-09-23 2006-12-06 심명식 악취생성방지 및 탈취활성을 가지는 락토바실러스 플란타룸ｃｕ03 ｋａｃｃ 91103
JP2008000118A (ja) * 2006-06-20 2008-01-10 Fujika Sogyo:Kk 家畜飼育場の殺菌方法
JP2009057284A (ja) * 2005-12-21 2009-03-19 Saihatsu Ko 家禽・家畜から排出される温室効果ガス生成抑制用微生物製剤

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08298982A (ja) * 1995-05-02 1996-11-19 Aasu Giken:Kk 複合微生物製剤
JPH09252728A (ja) * 1996-03-25 1997-09-30 Natl Fedelation Of Agricult Coop Assoc 消臭性飼料
JP2001299230A (ja) * 2000-04-27 2001-10-30 Korin Korea Co Ltd 飼料添加物およびその製造方法
JP2005065543A (ja) * 2003-08-21 2005-03-17 Oubiken:Kk 家畜の飼育床および家畜の飼育方法
JP2005245299A (ja) * 2004-03-04 2005-09-15 Bio Balance:Kk 新規な乳酸菌
KR100654427B1 (ko) * 2005-09-23 2006-12-06 심명식 악취생성방지 및 탈취활성을 가지는 락토바실러스 플란타룸ｃｕ03 ｋａｃｃ 91103
JP2009057284A (ja) * 2005-12-21 2009-03-19 Saihatsu Ko 家禽・家畜から排出される温室効果ガス生成抑制用微生物製剤
JP2008000118A (ja) * 2006-06-20 2008-01-10 Fujika Sogyo:Kk 家畜飼育場の殺菌方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHIHARU WATANABE ET AL.: "Nyusankin Shizai no Shiryo Tenka ga Keifun no Haisetsuryo to Shuki ni Oyobosu Eikyo", BULLETIN OF THE SHIGA PREFECTURAL LIVESTOCK RESEARCH AND IMPROVEMENT INSTITUTE, vol. 6, 1999, pages 34 - 39 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108546661A (zh) * 2018-04-26 2018-09-18 湖北华大瑞尔科技有限公司 一种抑菌型微生物发酵剂及其制备方法
CN108546661B (zh) * 2018-04-26 2021-10-08 湖北华大瑞尔科技有限公司 一种抑菌型微生物发酵剂的应用方法及其制备方法
CN112335556A (zh) * 2020-10-19 2021-02-09 广州动物园 一种新型圈养野生动物管理方法
CN112335556B (zh) * 2020-10-19 2022-05-06 广州动物园 一种新型圈养野生动物管理方法

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