WO2017038602A1 - Odour reduction method - Google Patents
Odour reduction method Download PDFInfo
- Publication number
- WO2017038602A1 WO2017038602A1 PCT/JP2016/074748 JP2016074748W WO2017038602A1 WO 2017038602 A1 WO2017038602 A1 WO 2017038602A1 JP 2016074748 W JP2016074748 W JP 2016074748W WO 2017038602 A1 WO2017038602 A1 WO 2017038602A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- lactic acid
- bacteria
- acid bacteria
- barn
- acd
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 35
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 266
- 241000894006 Bacteria Species 0.000 claims abstract description 200
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 132
- 239000004310 lactic acid Substances 0.000 claims abstract description 132
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 80
- 241000588769 Proteus <enterobacteria> Species 0.000 claims abstract description 46
- 238000005507 spraying Methods 0.000 claims abstract description 44
- 229910021529 ammonia Inorganic materials 0.000 claims abstract description 40
- 230000012010 growth Effects 0.000 claims abstract description 34
- 244000144972 livestock Species 0.000 claims abstract description 34
- 241001465754 Metazoa Species 0.000 claims abstract description 23
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 10
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 10
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 10
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 9
- 241000588724 Escherichia coli Species 0.000 claims description 16
- 239000003966 growth inhibitor Substances 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- 241000233866 Fungi Species 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 abstract description 8
- 210000003608 fece Anatomy 0.000 abstract description 7
- 108010046334 Urease Proteins 0.000 abstract description 6
- 230000002829 reductive effect Effects 0.000 abstract description 6
- 238000004140 cleaning Methods 0.000 abstract description 5
- 210000002700 urine Anatomy 0.000 abstract 2
- 229960000448 lactic acid Drugs 0.000 description 121
- 241000283690 Bos taurus Species 0.000 description 26
- 239000003795 chemical substances by application Substances 0.000 description 22
- 244000144977 poultry Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 239000011248 coating agent Substances 0.000 description 7
- 238000000576 coating method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 241000272184 Falconiformes Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000973887 Takayama Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- XUCIJNAGGSZNQT-JHSLDZJXSA-N (R)-amygdalin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H](C#N)C=2C=CC=CC=2)O1 XUCIJNAGGSZNQT-JHSLDZJXSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 description 1
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- WNBCMONIPIJTSB-BGNCJLHMSA-N Cichoriin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1c(O)cc2c(OC(=O)C=C2)c1 WNBCMONIPIJTSB-BGNCJLHMSA-N 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 1
- 241000272458 Numididae Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- 241000272534 Struthio camelus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 241001416177 Vicugna pacos Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 229940089837 amygdalin Drugs 0.000 description 1
- YZLOSXFCSIDECK-UHFFFAOYSA-N amygdalin Natural products OCC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC(C#N)c3ccccc3 YZLOSXFCSIDECK-UHFFFAOYSA-N 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 1
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- ABIUHPWEYMSGSR-UHFFFAOYSA-N bromocresol purple Chemical compound BrC1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(Br)C(O)=C(C)C=2)=C1 ABIUHPWEYMSGSR-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- -1 dversely Species 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 1
- 229940093496 esculin Drugs 0.000 description 1
- AWRMZKLXZLNBBK-UHFFFAOYSA-N esculin Natural products OC1OC(COc2cc3C=CC(=O)Oc3cc2O)C(O)C(O)C1O AWRMZKLXZLNBBK-UHFFFAOYSA-N 0.000 description 1
- YGHHWSRCTPQFFC-UHFFFAOYSA-N eucalyptosin A Natural products OC1C(O)C(O)C(CO)OC1OC1C(OC(C#N)C=2C=CC=CC=2)OC(CO)C(O)C1O YGHHWSRCTPQFFC-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000009408 flooring Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000176 sodium gluconate Substances 0.000 description 1
- 229940005574 sodium gluconate Drugs 0.000 description 1
- 235000012207 sodium gluconate Nutrition 0.000 description 1
- ZBCAZEFVTIBZJS-UHFFFAOYSA-M sodium;2-benzamidoacetate Chemical compound [Na+].[O-]C(=O)CNC(=O)C1=CC=CC=C1 ZBCAZEFVTIBZJS-UHFFFAOYSA-M 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Definitions
- the present invention relates to the field of reducing malodor caused by ammonia generated from manure of animals (particularly livestock animals).
- the present invention relates to the field of reducing malodors by suppressing the growth of microorganisms (especially microorganisms of the genus Proteus) that produce ammonia from animal manure.
- the genus Proteus is known as the family Enterobacteriaceae that resides in the stool with urease.
- Ammonia which is a malodorous component in barns, is known to be generated by the reaction of urea and urease in manure, and in order to prevent malodours in the barn, it is possible to prevent volatilization of ammonia by promptly cleaning the barn.
- Patent Document 1 lactic acid bacteria
- Patent Document 2 lactic acid bacteria compounding agents
- Patent Document 3 lactic acid bacteria compounding agents
- the present inventor has examined lactic acid bacteria having a proteus growth-inhibiting action in order to reduce the generation of ammonia by urease without cleaning the barn.
- lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD strain) ( NITE BP-02098) was found to effectively inhibit the growth of the genus Proteus.
- the present inventor sprayed the lactic acid bacteria BB-ACD strain on the flooring material, and the ammonia concentration in the barn decreased. I found out.
- the present inventor does not spray the lactic acid bacteria BB-ACD strain directly in the barn environment, but ingests the lactic acid bacteria BB-ACD strain in the manure by ingesting it as a feed.
- the ammonia concentration in the barn also decreased when the animal was ingested as feed.
- the present inventor has established a method for reducing malodor using lactic acid bacteria BB-ACD strain.
- the present invention is based on the finding of Lactobacillus acidophilus BB-ACD strain (NITE BP-02098), and specifically relates to the following invention.
- Lactobacillus acidophilus BB-ACD strain NITE BP-02098).
- a harmful bacteria growth inhibitor or malodor inhibitor containing the lactic acid bacterium according to (1) (2)
- the harmful bacteria growth inhibitor or malodor inhibitor according to (2), wherein the harmful bacteria are Escherichia coli and / or Proteus.
- the harmful fungus growth inhibitor or malodor suppressant according to (2) or (3) which is a preparation for spraying.
- a livestock feed comprising the lactic acid bacterium according to (1).
- a method for inhibiting the growth of harmful bacteria comprising spraying the lactic acid bacteria according to (1).
- a malodor reducing method comprising spraying the lactic acid bacteria according to (1).
- a malodor reducing method in a barn comprising spraying the lactic acid bacteria described in (1) on a floor in the barn.
- a malodor reducing method in a barn comprising feeding the lactic acid bacteria described in (1) to a livestock animal.
- the present invention relates to Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) and a harmful fungus growth inhibitor, a spray preparation, or a livestock feed containing the same.
- Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) is a lactic acid bacterium obtained by culturing from the small intestine of cattle in Takayama City, Gifu Prefecture, Japan. Deposited under the NITE BP-02098 at the National Institute of Technology Patent Microorganisms Deposit Center (2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture).
- livestock or livestock animals mean animals raised mainly for the purpose of using their products (milk, meat, eggs, hair, skin, fur, labor force, etc.). Including, horse, buffalo, deer, sheep, goat, alpaca, pig, eagle, rabbit, chicken, eagle, ostrich, turkey, goose, duck, pheasant, guinea fowl.
- the “harmful fungus” is not particularly limited as long as it is a fungus that exists in manure of livestock animals and adversely affects the growth of livestock. Harmful bacteria include malodor causing bacteria. Examples of harmful bacteria include urease-producing bacteria, preferably bacteria of the genus Escherichia coli and / or Proteus.
- the “harmful bacteria growth inhibitor” means a drug used for the purpose of inhibiting the growth of the harmful bacteria.
- the suppression of the growth of harmful bacteria does not mean that it is suppressed by 100%, and it is only required to be suppressed as compared with the drug non-spray group (or non-donating group), for example, 90%, 80% , 70% may be suppressed.
- Whether or not it is a harmful bacteria growth inhibitor is determined by, for example, spraying the test preparation once a week at 100 g / m 2 on a cow bed, 30 days after the start of the test (such as coliforms, As a control, measure the number of harmful bacteria (eg, coliforms, Proteus, lactic acid bacteria) in fattening cow beds that are not sprayed with lactic acid bacteria, and count the number of harmful bacteria. In comparison, it can be determined that there is an inhibitory effect on the growth of harmful bacteria when there are few harmful bacteria in the cow bed sprayed with the test preparation.
- harmful bacteria eg, coliforms, Proteus, lactic acid bacteria
- the harmful fungus growth inhibitor of the present invention may be in a form (spraying preparation) used by being directly sprayed on a livestock barn or the like, or in a form mixed with livestock feed (livestock feed).
- Formulation for spraying can be in liquid form or for spraying, and livestock feed can be in solid form such as tablet, granule or capsule, gel, liquid etc. according to the feeding method .
- the fattening cattle barn using wheat straw as the initial covering material was sprayed with 100 g / m 2 of the live lactic acid bacteria prepared in Example 2 once a week, and 30 days after the start of the test, It is a graph showing the result of having measured the number of Proteus genus, lactic acid bacteria.
- the number of bacteria Cold CFU / g
- the vertical axis represents the number of bacteria (log CFU / g)
- the horizontal axis represents bacteria.
- the graph shows the control on the left and the sprayed area on the right. In the same experiment as FIG.
- FIG. 3 it is a graph showing the result of having measured the ammonia density
- the vertical axis represents the ammonia concentration (ppm), and the horizontal axis represents the number of days elapsed since spraying (before spraying, the seventh day of spraying).
- the lactic acid bacteria viable preparation prepared in Example 2 was sprayed once at 100 g / m 2 on a flat laying house of laying hens. Three days after spraying, the bacteria on the floor of the poultry house (E.
- the graph shows the control on the left and the sprayed area on the right.
- FIG. 7 it is a graph showing the result of having measured the ammonia density
- This medium uses a medium suitable for lactic acid bacteria, the culture temperature is 20 to 45 ° C. (preferably 25 to 40 ° C.), and the culture pH is pH 3.5 to 9.0 (preferably 4. 5 to 7.0), and the culture time can be 6 to 30 hours.
- the bacterium can be inoculated into an MRS liquid medium and cultured at 30 to 40 ° C. for 24 hours.
- the culture solution can be sprayed on sterilized bran and cultured at 30 to 40 ° C. for 3 days.
- the toxic bacteria growth inhibitor or malodor control agent of the present invention can be used as it is in the case of either a spray preparation or a livestock feed. If necessary, an excipient may be added or dispersed in a liquid such as water.
- the present invention relates to the use of lactic acid bacteria BB-ACD strain for producing harmful bacteria growth inhibitors or malodor inhibitors for use in edible livestock or barns.
- the present invention also relates to a lactic acid bacterium BB-ACD strain for use in suppressing harmful bacteria growth or reducing malodor in edible livestock or barns.
- the present invention provides lactic acid bacteria BB-ACD strain (harmful bacteria growth inhibitor, or malodor suppressant, spraying preparation)
- the present invention relates to a method for inhibiting harmful bacteria growth or a method for reducing malodor.
- it is desirable that the lactic acid bacteria BB-ACD strain is directly sprayed on the place where the target harmful bacteria or malodor causing bacteria are generated.
- the spraying is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria, but, for example, once every two weeks or 1-2 times a week. (Preferably once a week), 1.0 ⁇ 10 10 to 1.0 ⁇ 10 12 CFU / m 2 (preferably 3.0 ⁇ 10 11 CFU / m 2 ) .
- a method for suppressing harmful bacteria growth in a barn and a method for reducing malodor comprising feeding a lactic acid bacteria BB-ACD strain to a livestock animal
- the present invention provides a lactic acid bacteria BB-ACD strain (a harmful bacteria growth inhibitor,
- the present invention relates to a method for suppressing the growth of harmful bacteria, or a method for reducing malodor, which comprises feeding livestock animals).
- the lactic acid bacteria BB-ACD strain is provided to livestock animals together with feed.
- the lactic acid bacteria BB-ACD strain can be given in solid form such as tablet, granule, capsule form, gel form, liquid form, etc. according to the feeding method as appropriate.
- the lactic acid bacteria BB-ACD strain is provided as live bacteria.
- the supply can be determined by the weight of the livestock animal, and is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria. In this case, it can be 10 to 100 g (preferably 40 g) per day.
- the period of feeding the feed of the present invention to livestock animals is not particularly limited, and can be changed according to the type of livestock animals, but can preferably be fed for the entire period of breeding. However, only part of the breeding period (one year, half year, three months, one month, etc.) may be paid. Except for feeding the feed of the present invention, livestock animals can be raised by commonly used breeding methods.
- Lactic acid bacteria BB-ACD strain Lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) was obtained by separating and culturing from the small intestine of cattle in Takayama, Gifu, Japan.
- the lactic acid bacteria BB-ACD strain is registered as NITE BP-02098 (date of trust: August 10th, 2015) with the Patent Microorganism Deposit Center (NPMD), National Institute of Technology and Evaluation (2-5-8, Kazusa-Kamashita, Chiba Prefecture). The day).
- the bacteriological properties of lactic acid bacteria (Lactobacillus acidophilus BB-ACD) cultured using MRS broth (manufactured by OXOID) are as follows. Regarding sugar assimilation, the basic medium for saccharide fermentability test (tripton 10.0 g, yeast extract 5.0 g, bromcresol purple 0.06 g was added distilled water to a total volume of 1000 ml, and adjusted to pH 6.8. , 121 ° C., high pressure sterilization for 15 minutes).
- Example 2 Preparation of Lactic Acid Bacteria BB-ACD Strain Bacteria
- the MRS liquid medium was inoculated with the present bacteria and cultured primarily at 30-40 ° C for 24 hours.
- the culture solution was sprayed onto a sterilized bran and subjected to secondary culture at 30 to 40 ° C. for 3 days to obtain a lactic acid bacteria viable agent.
- the number of lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) contained in the obtained lactic acid bacteria viable agent was 3.0 ⁇ 10 9 CFU / g.
- the lactic acid bacteria BB-ACD strain prepared in this example was used as the lactic acid bacteria.
- Example 3 Proliferation of Proteus by spraying live lactic acid bacteria and application to ammonia concentration in fattening cattle barn
- the live lactobacillus prepared in Example 2 was used in a fattening barn using wheat straw as an initial bedding material. It was sprayed at 100 g / m 2 once a week, and the number of cow bed bacteria (E. coli group, Proteus genus, lactic acid bacteria) was measured 30 days after the start of the test. As a control, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the fattening cow bed not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was sprayed was measured using a detector tube before spraying the lactic acid bacterium and before the 30th day of spraying.
- Example 4 Growth of Proteus by spraying live lactic acid bacteria and the effect on ammonia concentration in fattening piggery Lactic acid bacteria live fungus prepared in Example 2 in fattening piggery using sawdust as an initial covering material was sprayed at 100 g / m 2 once a week, and the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) in the pig bed was measured 7 days after the start of the test. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) in fattening pig beds not sprayed with a lactic acid bacteria viable agent was measured. Moreover, the ammonia concentration in the pig house which sprayed the lactic acid bacteria live bacteria agent was measured using the detection tube twice before the lactic acid bacteria live bacteria drug spraying.
- the lactic acid bacteria-sprayed pigbed had a higher number of lactic acid bacteria and fewer coliforms and Proteus than the lactic acid bacteria-spattered pigbed. Therefore, it was revealed that the growth of coliforms and Proteus in the pig bed was suppressed by the application of lactic acid bacteria. Moreover, in the pig house after lactic acid bacteria application
- Example 6 Examination of the effect of Proteus genus growth and ammonia concentration by feeding fattening cattle with a lactic acid bacteria viable agent 40 g of lactic acid bacteria preparation per day is mixed with normal feed to hybrid breeding cattle For 14 days, and the number of bacteria (coliform group, Proteus, lactic acid bacteria) in the cow bed before and after feeding was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the floor that raised fattening cows that were not fed with a lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was fed was measured using a detection tube twice before feeding the lactic bacterium and 14 days after spraying.
- the lactic acid bacteria BB-ACD strain is not only sprayed, but also when fed, it suppresses the growth of coliforms and Proteus on the barn floor and suppresses the production of ammonia in the barn to reduce malodor Was shown to do.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Animal Husbandry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
In order to reduce ammonia generation by urease without cleaning a livestock barn, the inventor of the present invention has provided lactic acid bacteria strain BB-ACD (Lactobacillus acidophilus strain BB-ACD) (NITE BP-02098) which exhibits Proteus growth-inhibiting action. The ammonia concentration in a livestock barn is reduced by spraying the lactic acid bacteria strain BB-ACD on bedding material. Furthermore, Proteus growth in faeces and urine can be directly inhibited by causing livestock animals to ingest the lactic acid bacteria strain BB-ACD as a feed, thereby causing the lactic acid bacteria strain BB-ACD to be included in the faeces and urine.
Description
本出願は、２０１５年８月３１日に日本国において出願された特願２０１５－１７０４６８号に基づき優先権を主張するものであり、当該出願に記載された内容の全体は、本明細書にそのまま援用される。また、本願において引用した特許、特許出願及び文献に記載された内容の全体は、本明細書にそのまま援用される。
This application claims priority based on Japanese Patent Application No. 2015-170468 filed in Japan on August 31, 2015, the entire contents of which are described in this specification as they are. Incorporated. In addition, the entire contents described in the patents, patent applications, and documents cited in the present application are incorporated in this specification as they are.
本発明は、動物（特に家畜動物）の糞尿から発生するアンモニアによる悪臭を低減させる分野に関する。特に本発明は、動物の糞尿からアンモニアを生成させる微生物（特には、Ｐｒｏｔｅｕｓ属の微生物）の生育を抑制することにより、悪臭を低減させる分野に関する。
The present invention relates to the field of reducing malodor caused by ammonia generated from manure of animals (particularly livestock animals). In particular, the present invention relates to the field of reducing malodors by suppressing the growth of microorganisms (especially microorganisms of the genus Proteus) that produce ammonia from animal manure.
Ｐｒｏｔｅｕｓ属は、ウレアーゼを有する糞便中に常在する腸内細菌科として知られている。畜舎の悪臭成分であるアンモニアは、糞尿中の尿素とウレアーゼが反応して発生することが知られており、畜舎の悪臭を防止するためには速やかに畜舎清掃をすることによってアンモニアの揮散を防ぐ必要がある。しかしながら、多頭管理をする畜産農家が頻繁に畜舎清掃をすることは負担が大きく、また、清掃毎に敷材を交換することから、交換敷材を多量に確保する必要があり、高額な費用がかかることが問題となっていた。
The genus Proteus is known as the family Enterobacteriaceae that resides in the stool with urease. Ammonia, which is a malodorous component in barns, is known to be generated by the reaction of urea and urease in manure, and in order to prevent malodours in the barn, it is possible to prevent volatilization of ammonia by promptly cleaning the barn. There is a need. However, it is burdensome for livestock farmers who manage many heads to clean their barns frequently, and it is necessary to secure a large amount of replacement laying material because the laying material is replaced for each cleaning. This has been a problem.
これまでも消臭を目的とした乳酸菌（特許文献１）や、乳酸菌配合剤（特許文献２及び３）が報告されているが、特に畜舎内の悪臭を抑制可能な優れた乳酸菌はこれまで報告されていなかった。
So far, lactic acid bacteria (Patent Document 1) and lactic acid bacteria compounding agents (Patent Documents 2 and 3) aimed at deodorization have been reported, but excellent lactic acid bacteria capable of suppressing malodor in barns have been reported so far. Was not.
本発明者は畜舎清掃を行うことなくウレアーゼによるアンモニア発生を低減させるために、プロテウス属の生育抑制作用がある乳酸菌について検討を行った結果、乳酸菌ＢＢ－ＡＣＤ株（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株）（ＮＩＴＥ ＢＰ－０２０９８）が効果的にＰｒｏｔｅｕｓ属の増殖を抑制することを見出した。また、本発明者は、実際に乳酸菌ＢＢ－ＡＣＤ株を畜舎環境中で悪臭を抑制できる否かを調べるため、乳酸菌ＢＢ－ＡＣＤ株を敷材に散布したところ、畜舎内のアンモニア濃度が低下することを見出した。更に、本発明者は、乳酸菌ＢＢ－ＡＣＤ株を畜舎環境中に直接散布するのではなく、飼料として畜産動物に摂取させることにより、糞尿中に乳酸菌ＢＢ－ＡＣＤ株を含有せしめ、糞尿中にて直接プロテウス属の生育を抑制可能であるか否かを確認したところ、飼料として畜産動物に摂取させた場合にも、畜舎内のアンモニア濃度が低下することを見出した。これらの研究により、本発明者は乳酸菌ＢＢ－ＡＣＤ株を用いた悪臭の低減方法を確立した。
The present inventor has examined lactic acid bacteria having a proteus growth-inhibiting action in order to reduce the generation of ammonia by urease without cleaning the barn. As a result, lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD strain) ( NITE BP-02098) was found to effectively inhibit the growth of the genus Proteus. In addition, in order to examine whether or not the lactic acid bacteria BB-ACD strain can be actually suppressed in the barn environment, the present inventor sprayed the lactic acid bacteria BB-ACD strain on the flooring material, and the ammonia concentration in the barn decreased. I found out. Furthermore, the present inventor does not spray the lactic acid bacteria BB-ACD strain directly in the barn environment, but ingests the lactic acid bacteria BB-ACD strain in the manure by ingesting it as a feed. When it was confirmed whether or not the growth of the genus Proteus could be directly suppressed, it was found that the ammonia concentration in the barn also decreased when the animal was ingested as feed. Based on these studies, the present inventor has established a method for reducing malodor using lactic acid bacteria BB-ACD strain.
すなわち、本発明は、Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）を見出したことに基づくものであり、具体的には以下の発明に関する。
（１） Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）。
（２） （１）に記載の乳酸菌を含有する、有害菌増殖抑制剤又は悪臭抑制剤。
（３） 前記有害菌が、大腸菌及び／又はプロテウス属である、（２）に記載の有害菌増殖抑制剤又は悪臭抑制剤。
（４） 散布用製剤である、（２）又は（３）に記載の有害菌増殖抑制剤又は悪臭抑制剤。
（５） （１）に記載の乳酸菌を含有する、家畜用飼料。
（６） （１）に記載の乳酸菌を散布することを備える、有害菌増殖抑制方法。
（７） （１）に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の床における有害菌増殖抑制方法。
（８） （１）に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における有害菌増殖を抑制する方法。
（９） 家畜動物が牛である、（８）に記載の方法。
（１０） 前記有害菌が、大腸菌及び／又はプロテウス属である、（６）～（９）のいずれか１項に記載の方法。
（１１） （１）に記載の乳酸菌を散布することを備える、悪臭低減方法。
（１２） （１）に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の悪臭低減方法。
（１３） （１）に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における悪臭低減方法。
（１４） 家畜動物が牛である、（１３）に記載の方法。
（１５） 悪臭が、アンモニアによる悪臭である、（１１）～（１４）のいずれか１項に記載の方法。 That is, the present invention is based on the finding of Lactobacillus acidophilus BB-ACD strain (NITE BP-02098), and specifically relates to the following invention.
(1) Lactobacillus acidophilus BB-ACD strain (NITE BP-02098).
(2) A harmful bacteria growth inhibitor or malodor inhibitor containing the lactic acid bacterium according to (1).
(3) The harmful bacteria growth inhibitor or malodor inhibitor according to (2), wherein the harmful bacteria are Escherichia coli and / or Proteus.
(4) The harmful fungus growth inhibitor or malodor suppressant according to (2) or (3), which is a preparation for spraying.
(5) A livestock feed comprising the lactic acid bacterium according to (1).
(6) A method for inhibiting the growth of harmful bacteria, comprising spraying the lactic acid bacteria according to (1).
(7) A method for inhibiting harmful bacteria growth on a floor in a barn, comprising spraying the lactic acid bacteria described in (1) on the floor in a barn.
(8) A method for suppressing the growth of harmful bacteria in a barn, comprising feeding the lactic acid bacteria described in (1) to livestock animals.
(9) The method according to (8), wherein the domestic animal is a cow.
(10) The method according to any one of (6) to (9), wherein the harmful bacteria are Escherichia coli and / or Proteus.
(11) A malodor reducing method comprising spraying the lactic acid bacteria according to (1).
(12) A malodor reducing method in a barn, comprising spraying the lactic acid bacteria described in (1) on a floor in the barn.
(13) A malodor reducing method in a barn comprising feeding the lactic acid bacteria described in (1) to a livestock animal.
(14) The method according to (13), wherein the domestic animal is a cow.
(15) The method according to any one of (11) to (14), wherein the malodor is a malodor due to ammonia.
（１） Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）。
（２） （１）に記載の乳酸菌を含有する、有害菌増殖抑制剤又は悪臭抑制剤。
（３） 前記有害菌が、大腸菌及び／又はプロテウス属である、（２）に記載の有害菌増殖抑制剤又は悪臭抑制剤。
（４） 散布用製剤である、（２）又は（３）に記載の有害菌増殖抑制剤又は悪臭抑制剤。
（５） （１）に記載の乳酸菌を含有する、家畜用飼料。
（６） （１）に記載の乳酸菌を散布することを備える、有害菌増殖抑制方法。
（７） （１）に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の床における有害菌増殖抑制方法。
（８） （１）に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における有害菌増殖を抑制する方法。
（９） 家畜動物が牛である、（８）に記載の方法。
（１０） 前記有害菌が、大腸菌及び／又はプロテウス属である、（６）～（９）のいずれか１項に記載の方法。
（１１） （１）に記載の乳酸菌を散布することを備える、悪臭低減方法。
（１２） （１）に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の悪臭低減方法。
（１３） （１）に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における悪臭低減方法。
（１４） 家畜動物が牛である、（１３）に記載の方法。
（１５） 悪臭が、アンモニアによる悪臭である、（１１）～（１４）のいずれか１項に記載の方法。 That is, the present invention is based on the finding of Lactobacillus acidophilus BB-ACD strain (NITE BP-02098), and specifically relates to the following invention.
(1) Lactobacillus acidophilus BB-ACD strain (NITE BP-02098).
(2) A harmful bacteria growth inhibitor or malodor inhibitor containing the lactic acid bacterium according to (1).
(3) The harmful bacteria growth inhibitor or malodor inhibitor according to (2), wherein the harmful bacteria are Escherichia coli and / or Proteus.
(4) The harmful fungus growth inhibitor or malodor suppressant according to (2) or (3), which is a preparation for spraying.
(5) A livestock feed comprising the lactic acid bacterium according to (1).
(6) A method for inhibiting the growth of harmful bacteria, comprising spraying the lactic acid bacteria according to (1).
(7) A method for inhibiting harmful bacteria growth on a floor in a barn, comprising spraying the lactic acid bacteria described in (1) on the floor in a barn.
(8) A method for suppressing the growth of harmful bacteria in a barn, comprising feeding the lactic acid bacteria described in (1) to livestock animals.
(9) The method according to (8), wherein the domestic animal is a cow.
(10) The method according to any one of (6) to (9), wherein the harmful bacteria are Escherichia coli and / or Proteus.
(11) A malodor reducing method comprising spraying the lactic acid bacteria according to (1).
(12) A malodor reducing method in a barn, comprising spraying the lactic acid bacteria described in (1) on a floor in the barn.
(13) A malodor reducing method in a barn comprising feeding the lactic acid bacteria described in (1) to a livestock animal.
(14) The method according to (13), wherein the domestic animal is a cow.
(15) The method according to any one of (11) to (14), wherein the malodor is a malodor due to ammonia.
よって、一態様において、本発明は、Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）及びそれを含有する有害菌増殖抑制剤、散布用製剤又は家畜用飼料に関する。Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）は、日本国岐阜県高山市の牛の小腸から分離培養することにより取得した乳酸菌であり、２０１５年８月１０日付にて、独立行政法人製品評価技術基盤機構 特許微生物寄託センター（千葉県木更津市かずさ鎌足２－５－８）に受託番号ＮＩＴＥ ＢＰ－０２０９８として寄託されている。
Therefore, in one aspect, the present invention relates to Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) and a harmful fungus growth inhibitor, a spray preparation, or a livestock feed containing the same. Lactobacillus acidophilus BB-ACD strain (NITE BP-02098) is a lactic acid bacterium obtained by culturing from the small intestine of cattle in Takayama City, Gifu Prefecture, Japan. Deposited under the NITE BP-02098 at the National Institute of Technology Patent Microorganisms Deposit Center (2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture).
本明細書において、家畜又は家畜動物とは、主にその生産物（乳、肉、卵、毛、皮、毛皮、労働力など）の利用を目的として飼育される動物を意味し、例えば、牛、馬、水牛、鹿、羊、山羊、アルパカ、豚、猪、ウサギ、鶏、鶉、ダチョウ、七面鳥、ガチョウ、アヒル、キジ、ホロホロ鳥を含む。
In this specification, livestock or livestock animals mean animals raised mainly for the purpose of using their products (milk, meat, eggs, hair, skin, fur, labor force, etc.). Including, horse, buffalo, deer, sheep, goat, alpaca, pig, eagle, rabbit, chicken, eagle, ostrich, turkey, goose, duck, pheasant, guinea fowl.
本明細書において、「有害菌」とは、家畜動物の糞尿中に存在し、家畜の生育に悪影響を与える菌であれば特に制限されるものではない。有害菌は悪臭の原因菌を含む。有害菌としては、例えば、ウレアーゼを産生する菌を挙げることができ、好ましくは、大腸菌及び／又はＰｒｏｔｅｕｓ属の菌である。
In the present specification, the “harmful fungus” is not particularly limited as long as it is a fungus that exists in manure of livestock animals and adversely affects the growth of livestock. Harmful bacteria include malodor causing bacteria. Examples of harmful bacteria include urease-producing bacteria, preferably bacteria of the genus Escherichia coli and / or Proteus.
本明細書において、「有害菌増殖抑制剤」とは、前記有害菌の増殖を抑制する目的で使用される薬剤を意味する。有害菌の増殖抑制は、１００％抑制されることを意味するものではなく、当該薬剤非散布群（又は非供与群）と比較して、抑制されていればよく、例えば、９０％、８０％、７０％抑制することを意味していてもよい。有害菌増殖抑制剤であるか否かは、例えば、被験製剤を１週間に１回、１００ｇ／ｍ２で牛床に散布し、試験開始３０日後に牛床の有害菌（例えば、大腸菌群、プロテウス属）の数を測定し、コントロールとして、乳酸菌生菌剤を散布していない肥育牛床の有害菌（例えば、大腸菌群、プロテウス属、乳酸菌）の数を測定し、それぞれの有害菌の数を比較して、被験製剤を散布した牛床の有害菌が少ない場合に有害菌増殖抑制作用があると決定することができる。
In the present specification, the “harmful bacteria growth inhibitor” means a drug used for the purpose of inhibiting the growth of the harmful bacteria. The suppression of the growth of harmful bacteria does not mean that it is suppressed by 100%, and it is only required to be suppressed as compared with the drug non-spray group (or non-donating group), for example, 90%, 80% , 70% may be suppressed. Whether or not it is a harmful bacteria growth inhibitor is determined by, for example, spraying the test preparation once a week at 100 g / m 2 on a cow bed, 30 days after the start of the test (such as coliforms, As a control, measure the number of harmful bacteria (eg, coliforms, Proteus, lactic acid bacteria) in fattening cow beds that are not sprayed with lactic acid bacteria, and count the number of harmful bacteria. In comparison, it can be determined that there is an inhibitory effect on the growth of harmful bacteria when there are few harmful bacteria in the cow bed sprayed with the test preparation.
本発明の有害菌増殖抑制剤は、畜舎等に直接散布して用いる形態（散布用製剤）であってもよいし、家畜用の餌に混ぜた形態（家畜用飼料）であってもよい。散布用製剤は、液体状やスプレー用とすることができ、家畜用飼料は、給餌方法に合わせて、タブレット、顆粒状、カプセル状などの固形状、ゲル状、液体状などとすることができる。
The harmful fungus growth inhibitor of the present invention may be in a form (spraying preparation) used by being directly sprayed on a livestock barn or the like, or in a form mixed with livestock feed (livestock feed). Formulation for spraying can be in liquid form or for spraying, and livestock feed can be in solid form such as tablet, granule or capsule, gel, liquid etc. according to the feeding method .
本発明は乳酸菌ＢＢ－ＡＣＤ株を用いることにより、畜舎清掃を行うことなくウレアーゼによるアンモニア発生を低減させることができる。具体的には、乳酸菌ＢＢ－ＡＣＤ株は、効果的にＰｒｏｔｅｕｓ属及び大腸菌の増殖を抑制し、畜舎内のアンモニア発生を低下させることから、多頭管理をする畜産農家であっても頻繁に畜舎清掃を行うことなく、安価に畜舎内の悪臭を低減させることができる。
In the present invention, by using the lactic acid bacteria BB-ACD strain, generation of ammonia by urease can be reduced without cleaning the barn. Specifically, the lactic acid bacteria BB-ACD strain effectively suppresses the growth of the genus Proteus and E. coli and reduces ammonia generation in the barn. It is possible to reduce the bad odor in the barn at a low cost without performing the operation.
１．乳酸菌ＢＢ－ＡＣＤ株の培養
本菌は、乳酸菌に適した培地を用い、培養温度は、２０～４５℃（好ましく２５～４０℃）、培養ｐＨはｐＨ３．５～９．０（好ましくは４．５～７．０）、培養時間は６～３０時間とすることで培養することができる。具体例としては、ＭＲＳ液体培地に本菌を接種し、３０～４０℃で２４時間培養することができる。更に、２次培養する場合には、培養液を滅菌されたふすまに噴霧し、３０～４０℃で３日間培養することができる。 1. Cultivation of Lactic Acid Bacteria BB-ACD This medium uses a medium suitable for lactic acid bacteria, the culture temperature is 20 to 45 ° C. (preferably 25 to 40 ° C.), and the culture pH is pH 3.5 to 9.0 (preferably 4. 5 to 7.0), and the culture time can be 6 to 30 hours. As a specific example, the bacterium can be inoculated into an MRS liquid medium and cultured at 30 to 40 ° C. for 24 hours. Furthermore, in the case of secondary culture, the culture solution can be sprayed on sterilized bran and cultured at 30 to 40 ° C. for 3 days.
本菌は、乳酸菌に適した培地を用い、培養温度は、２０～４５℃（好ましく２５～４０℃）、培養ｐＨはｐＨ３．５～９．０（好ましくは４．５～７．０）、培養時間は６～３０時間とすることで培養することができる。具体例としては、ＭＲＳ液体培地に本菌を接種し、３０～４０℃で２４時間培養することができる。更に、２次培養する場合には、培養液を滅菌されたふすまに噴霧し、３０～４０℃で３日間培養することができる。 1. Cultivation of Lactic Acid Bacteria BB-ACD This medium uses a medium suitable for lactic acid bacteria, the culture temperature is 20 to 45 ° C. (preferably 25 to 40 ° C.), and the culture pH is pH 3.5 to 9.0 (preferably 4. 5 to 7.0), and the culture time can be 6 to 30 hours. As a specific example, the bacterium can be inoculated into an MRS liquid medium and cultured at 30 to 40 ° C. for 24 hours. Furthermore, in the case of secondary culture, the culture solution can be sprayed on sterilized bran and cultured at 30 to 40 ° C. for 3 days.
２．有害菌増殖抑制剤等
本発明の有害菌増殖抑制剤又は悪臭抑制剤は、散布用製剤又は家畜用飼料のいずれの場合にも、前記方法で培養された乳酸菌製剤をそのまま使用することもできるし、必要に応じて、賦形剤を添加したり、水などの液体に分散させて調製してもよい。一態様において、本発明は、食用家畜又は畜舎で用いられる有害菌増殖抑制剤又は悪臭抑制剤を製造するための、乳酸菌ＢＢ－ＡＣＤ株の使用に関する。また、本発明は、食用家畜又は畜舎における有害菌増殖を抑制し又は悪臭を低減させることに使用するための乳酸菌ＢＢ－ＡＣＤ株に関する。 2. Toxic Bacterial Growth Inhibitors, etc. The toxic bacteria growth inhibitor or malodor control agent of the present invention can be used as it is in the case of either a spray preparation or a livestock feed. If necessary, an excipient may be added or dispersed in a liquid such as water. In one aspect, the present invention relates to the use of lactic acid bacteria BB-ACD strain for producing harmful bacteria growth inhibitors or malodor inhibitors for use in edible livestock or barns. The present invention also relates to a lactic acid bacterium BB-ACD strain for use in suppressing harmful bacteria growth or reducing malodor in edible livestock or barns.
本発明の有害菌増殖抑制剤又は悪臭抑制剤は、散布用製剤又は家畜用飼料のいずれの場合にも、前記方法で培養された乳酸菌製剤をそのまま使用することもできるし、必要に応じて、賦形剤を添加したり、水などの液体に分散させて調製してもよい。一態様において、本発明は、食用家畜又は畜舎で用いられる有害菌増殖抑制剤又は悪臭抑制剤を製造するための、乳酸菌ＢＢ－ＡＣＤ株の使用に関する。また、本発明は、食用家畜又は畜舎における有害菌増殖を抑制し又は悪臭を低減させることに使用するための乳酸菌ＢＢ－ＡＣＤ株に関する。 2. Toxic Bacterial Growth Inhibitors, etc. The toxic bacteria growth inhibitor or malodor control agent of the present invention can be used as it is in the case of either a spray preparation or a livestock feed. If necessary, an excipient may be added or dispersed in a liquid such as water. In one aspect, the present invention relates to the use of lactic acid bacteria BB-ACD strain for producing harmful bacteria growth inhibitors or malodor inhibitors for use in edible livestock or barns. The present invention also relates to a lactic acid bacterium BB-ACD strain for use in suppressing harmful bacteria growth or reducing malodor in edible livestock or barns.
３．乳酸菌ＢＢ－ＡＣＤ株を散布することを備える、有害菌増殖抑制方法及び悪臭低減方法
一態様において、本発明は、乳酸菌ＢＢ－ＡＣＤ株（有害菌増殖抑制剤、又は悪臭抑制剤、散布用製剤）を散布することを備える、有害菌増殖抑制方法、又は悪臭低減方法に関する。本方法において、乳酸菌ＢＢ－ＡＣＤ株は対象となる有害菌又は悪臭の原因菌が発生する場所に直接散布することが望ましい。散布は、乳酸菌が十分有害菌又は悪臭の原因菌の増殖を抑制可能となる細胞濃度及び頻度であれば特に制限されるものではないが、例えば、２週間に１回又は１週間に１～２回（好ましくは、１週間に１回）、１．０×１０１０～１．０×１０１２ＣＦＵ／ｍ２（好ましくは、３．０×１０１１ＣＦＵ／ｍ２）で散布することができる。 3. Method for suppressing harmful bacteria growth and method for reducing malodor comprising spraying lactic acid bacteria BB-ACD strain In one aspect, the present invention provides lactic acid bacteria BB-ACD strain (harmful bacteria growth inhibitor, or malodor suppressant, spraying preparation) The present invention relates to a method for inhibiting harmful bacteria growth or a method for reducing malodor. In this method, it is desirable that the lactic acid bacteria BB-ACD strain is directly sprayed on the place where the target harmful bacteria or malodor causing bacteria are generated. The spraying is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria, but, for example, once every two weeks or 1-2 times a week. (Preferably once a week), 1.0 × 10 10 to 1.0 × 10 12 CFU / m 2 (preferably 3.0 × 10 11 CFU / m 2 ) .
一態様において、本発明は、乳酸菌ＢＢ－ＡＣＤ株（有害菌増殖抑制剤、又は悪臭抑制剤、散布用製剤）を散布することを備える、有害菌増殖抑制方法、又は悪臭低減方法に関する。本方法において、乳酸菌ＢＢ－ＡＣＤ株は対象となる有害菌又は悪臭の原因菌が発生する場所に直接散布することが望ましい。散布は、乳酸菌が十分有害菌又は悪臭の原因菌の増殖を抑制可能となる細胞濃度及び頻度であれば特に制限されるものではないが、例えば、２週間に１回又は１週間に１～２回（好ましくは、１週間に１回）、１．０×１０１０～１．０×１０１２ＣＦＵ／ｍ２（好ましくは、３．０×１０１１ＣＦＵ／ｍ２）で散布することができる。 3. Method for suppressing harmful bacteria growth and method for reducing malodor comprising spraying lactic acid bacteria BB-ACD strain In one aspect, the present invention provides lactic acid bacteria BB-ACD strain (harmful bacteria growth inhibitor, or malodor suppressant, spraying preparation) The present invention relates to a method for inhibiting harmful bacteria growth or a method for reducing malodor. In this method, it is desirable that the lactic acid bacteria BB-ACD strain is directly sprayed on the place where the target harmful bacteria or malodor causing bacteria are generated. The spraying is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria, but, for example, once every two weeks or 1-2 times a week. (Preferably once a week), 1.0 × 10 10 to 1.0 × 10 12 CFU / m 2 (preferably 3.0 × 10 11 CFU / m 2 ) .
４．乳酸菌ＢＢ－ＡＣＤ株を家畜動物に給与することを備える、畜舎内における有害菌増殖を抑制する方法、及び悪臭低減方法
一態様において、本発明は、乳酸菌ＢＢ－ＡＣＤ株（有害菌増殖抑制剤、家畜用飼料）を家畜動物に給与することを備える、有害菌増殖抑制方法、又は悪臭低減方法に関する。本方法において、乳酸菌ＢＢ－ＡＣＤ株は飼料と共に家畜動物に供与される。乳酸菌ＢＢ－ＡＣＤ株は、適宜給餌方法に合わせて、タブレット、顆粒状、カプセル状などの固形状、ゲル状、液体状などで与えることができる。好ましくは、乳酸菌ＢＢ－ＡＣＤ株は、生菌のまま供与される。供与は、家畜動物の体重によって決定することができ、乳酸菌が十分有害菌又は悪臭の原因菌の増殖を抑制可能となる細胞濃度及び頻度であれば特に制限されるものではないが、例えば、牛の場合、１日１０～１００ｇ（好ましくは、４０ｇ）とすることができる。本発明の飼料の家畜動物への給与の期間は特に限定されるものではなく、家畜動物の種類に応じて変更することができるが、好ましくは飼育の全期間給与することができる。ただし、飼育期間の一部（１年間、半年間、３ヶ月間、１月間など）のみ給与してもよい。本発明の飼料を給与すること以外は、家畜動物は通常用いられる飼育方法により飼育することができる。 4). A method for suppressing harmful bacteria growth in a barn and a method for reducing malodor comprising feeding a lactic acid bacteria BB-ACD strain to a livestock animal In one aspect, the present invention provides a lactic acid bacteria BB-ACD strain (a harmful bacteria growth inhibitor, The present invention relates to a method for suppressing the growth of harmful bacteria, or a method for reducing malodor, which comprises feeding livestock animals). In this method, the lactic acid bacteria BB-ACD strain is provided to livestock animals together with feed. The lactic acid bacteria BB-ACD strain can be given in solid form such as tablet, granule, capsule form, gel form, liquid form, etc. according to the feeding method as appropriate. Preferably, the lactic acid bacteria BB-ACD strain is provided as live bacteria. The supply can be determined by the weight of the livestock animal, and is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria. In this case, it can be 10 to 100 g (preferably 40 g) per day. The period of feeding the feed of the present invention to livestock animals is not particularly limited, and can be changed according to the type of livestock animals, but can preferably be fed for the entire period of breeding. However, only part of the breeding period (one year, half year, three months, one month, etc.) may be paid. Except for feeding the feed of the present invention, livestock animals can be raised by commonly used breeding methods.
一態様において、本発明は、乳酸菌ＢＢ－ＡＣＤ株（有害菌増殖抑制剤、家畜用飼料）を家畜動物に給与することを備える、有害菌増殖抑制方法、又は悪臭低減方法に関する。本方法において、乳酸菌ＢＢ－ＡＣＤ株は飼料と共に家畜動物に供与される。乳酸菌ＢＢ－ＡＣＤ株は、適宜給餌方法に合わせて、タブレット、顆粒状、カプセル状などの固形状、ゲル状、液体状などで与えることができる。好ましくは、乳酸菌ＢＢ－ＡＣＤ株は、生菌のまま供与される。供与は、家畜動物の体重によって決定することができ、乳酸菌が十分有害菌又は悪臭の原因菌の増殖を抑制可能となる細胞濃度及び頻度であれば特に制限されるものではないが、例えば、牛の場合、１日１０～１００ｇ（好ましくは、４０ｇ）とすることができる。本発明の飼料の家畜動物への給与の期間は特に限定されるものではなく、家畜動物の種類に応じて変更することができるが、好ましくは飼育の全期間給与することができる。ただし、飼育期間の一部（１年間、半年間、３ヶ月間、１月間など）のみ給与してもよい。本発明の飼料を給与すること以外は、家畜動物は通常用いられる飼育方法により飼育することができる。 4). A method for suppressing harmful bacteria growth in a barn and a method for reducing malodor comprising feeding a lactic acid bacteria BB-ACD strain to a livestock animal In one aspect, the present invention provides a lactic acid bacteria BB-ACD strain (a harmful bacteria growth inhibitor, The present invention relates to a method for suppressing the growth of harmful bacteria, or a method for reducing malodor, which comprises feeding livestock animals). In this method, the lactic acid bacteria BB-ACD strain is provided to livestock animals together with feed. The lactic acid bacteria BB-ACD strain can be given in solid form such as tablet, granule, capsule form, gel form, liquid form, etc. according to the feeding method as appropriate. Preferably, the lactic acid bacteria BB-ACD strain is provided as live bacteria. The supply can be determined by the weight of the livestock animal, and is not particularly limited as long as the lactic acid bacteria can sufficiently suppress the growth of harmful bacteria or malodor-causing bacteria. In this case, it can be 10 to 100 g (preferably 40 g) per day. The period of feeding the feed of the present invention to livestock animals is not particularly limited, and can be changed according to the type of livestock animals, but can preferably be fed for the entire period of breeding. However, only part of the breeding period (one year, half year, three months, one month, etc.) may be paid. Except for feeding the feed of the present invention, livestock animals can be raised by commonly used breeding methods.
以下、本発明をより詳細に説明するため実施例を示すが、本発明はこれに限定されるものではない。なお、本願全体を通して引用される全文献は参照によりそのまま本願に組み込まれる。
Hereinafter, examples will be shown in order to explain the present invention in more detail, but the present invention is not limited thereto. It should be noted that all documents cited throughout this application are incorporated herein by reference in their entirety.
（実施例１）乳酸菌ＢＢ－ＡＣＤ株
乳酸菌ＢＢ－ＡＣＤ株（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ）は、日本国岐阜県高山市の牛の小腸から分離培養することにより取得した。乳酸菌ＢＢ－ＡＣＤ株は、独立行政法人製品評価技術基盤機構特許微生物寄託センター（ＮＰＭＤ）（千葉県木更津市かずさ鎌足２－５－８）受託番号ＮＩＴＥ ＢＰ－０２０９８（受託日２０１５年８月１０日）として寄託されている。 (Example 1) Lactic acid bacteria BB-ACD strain Lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) was obtained by separating and culturing from the small intestine of cattle in Takayama, Gifu, Japan. The lactic acid bacteria BB-ACD strain is registered as NITE BP-02098 (date of trust: August 10th, 2015) with the Patent Microorganism Deposit Center (NPMD), National Institute of Technology and Evaluation (2-5-8, Kazusa-Kamashita, Chiba Prefecture). The day).
乳酸菌ＢＢ－ＡＣＤ株（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ）は、日本国岐阜県高山市の牛の小腸から分離培養することにより取得した。乳酸菌ＢＢ－ＡＣＤ株は、独立行政法人製品評価技術基盤機構特許微生物寄託センター（ＮＰＭＤ）（千葉県木更津市かずさ鎌足２－５－８）受託番号ＮＩＴＥ ＢＰ－０２０９８（受託日２０１５年８月１０日）として寄託されている。 (Example 1) Lactic acid bacteria BB-ACD strain Lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) was obtained by separating and culturing from the small intestine of cattle in Takayama, Gifu, Japan. The lactic acid bacteria BB-ACD strain is registered as NITE BP-02098 (date of trust: August 10th, 2015) with the Patent Microorganism Deposit Center (NPMD), National Institute of Technology and Evaluation (2-5-8, Kazusa-Kamashita, Chiba Prefecture). The day).
ＭＲＳブロス（ＯＸＯＩＤ社製）を用いて培養した乳酸菌（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ）の菌学的性質は以下の通りである。なお、糖資化性については、糖類発酵性試験用基礎培地（トリプトン１０．０ｇ、酵母エキス５．０ｇ、ブロムクレゾールパープル０．０６ｇに蒸留水を加えて全量１０００ｍｌとし、ｐＨ６．８に調整後、１２１℃、１５分間高圧滅菌）を用いて培養した。
細胞の形態：桿菌
胞子形成：無
運動性：無
グラム染色：＋
酸素に対する感受性：通性嫌気性
１５℃での生育：－
４５℃での生育：＋
乳酸発酵： ホモ型
グルコースからのガスの生成：－
アルギニンからのアンモニアの生成：－
馬尿酸ナトリウムの加水分解性：－
４％塩化ナトリウム耐性：－
糖資化性：アラビノース（－）、キシロース（－）、ラムノース（－）、リボース（－）、グルコース（＋）、マンノース（＋）、フルクトース（＋）、ガラクトース（＋）、スクロース（＋）、マルトース（＋）、セロビオース（＋）、ラクトース（＋）、トレハロース（＋）、メリビオース（－）、ラフィノース（－）、メレチトース（－）、マンニトール（－）、ソルビトール（－）、エスクリン（＋）、サリシン（＋）、アミグダリン（－）、グルコン酸ナトリウム（－）
生成乳酸：ＤＬ乳酸 The bacteriological properties of lactic acid bacteria (Lactobacillus acidophilus BB-ACD) cultured using MRS broth (manufactured by OXOID) are as follows. Regarding sugar assimilation, the basic medium for saccharide fermentability test (tripton 10.0 g, yeast extract 5.0 g, bromcresol purple 0.06 g was added distilled water to a total volume of 1000 ml, and adjusted to pH 6.8. , 121 ° C., high pressure sterilization for 15 minutes).
Cell morphology: Neisseria gonorrhoeae Sporulation: No Motility: No Gram staining: +
Sensitivity to oxygen: facultative anaerobic growth at 15 ° C:-
Growth at 45 ° C: +
Lactic acid fermentation: Homogeneous gas production from glucose:-
Production of ammonia from arginine:-
Hydrolysis of sodium hippurate:-
4% sodium chloride tolerance:-
Glucose utilization: arabinose (-), xylose (-), rhamnose (-), ribose (-), glucose (+), mannose (+), fructose (+), galactose (+), sucrose (+), Maltose (+), cellobiose (+), lactose (+), trehalose (+), melibiose (-), raffinose (-), meletitol (-), mannitol (-), sorbitol (-), esculin (+), Salicin (+), amygdalin (-), sodium gluconate (-)
Produced lactic acid: DL lactic acid
細胞の形態：桿菌
胞子形成：無
運動性：無
グラム染色：＋
酸素に対する感受性：通性嫌気性
１５℃での生育：－
４５℃での生育：＋
乳酸発酵： ホモ型
グルコースからのガスの生成：－
アルギニンからのアンモニアの生成：－
馬尿酸ナトリウムの加水分解性：－
４％塩化ナトリウム耐性：－
糖資化性：アラビノース（－）、キシロース（－）、ラムノース（－）、リボース（－）、グルコース（＋）、マンノース（＋）、フルクトース（＋）、ガラクトース（＋）、スクロース（＋）、マルトース（＋）、セロビオース（＋）、ラクトース（＋）、トレハロース（＋）、メリビオース（－）、ラフィノース（－）、メレチトース（－）、マンニトール（－）、ソルビトール（－）、エスクリン（＋）、サリシン（＋）、アミグダリン（－）、グルコン酸ナトリウム（－）
生成乳酸：ＤＬ乳酸 The bacteriological properties of lactic acid bacteria (Lactobacillus acidophilus BB-ACD) cultured using MRS broth (manufactured by OXOID) are as follows. Regarding sugar assimilation, the basic medium for saccharide fermentability test (tripton 10.0 g, yeast extract 5.0 g, bromcresol purple 0.06 g was added distilled water to a total volume of 1000 ml, and adjusted to pH 6.8. , 121 ° C., high pressure sterilization for 15 minutes).
Cell morphology: Neisseria gonorrhoeae Sporulation: No Motility: No Gram staining: +
Sensitivity to oxygen: facultative anaerobic growth at 15 ° C:-
Growth at 45 ° C: +
Lactic acid fermentation: Homogeneous gas production from glucose:-
Production of ammonia from arginine:-
Hydrolysis of sodium hippurate:-
4% sodium chloride tolerance:-
Glucose utilization: arabinose (-), xylose (-), rhamnose (-), ribose (-), glucose (+), mannose (+), fructose (+), galactose (+), sucrose (+), Maltose (+), cellobiose (+), lactose (+), trehalose (+), melibiose (-), raffinose (-), meletitol (-), mannitol (-), sorbitol (-), esculin (+), Salicin (+), amygdalin (-), sodium gluconate (-)
Produced lactic acid: DL lactic acid
（実施例２）乳酸菌ＢＢ－ＡＣＤ株生菌剤の調製
ＭＲＳ液体培地に本菌を接種し、３０～４０℃で２４時間１次培養した。培養液を滅菌されたふすまに噴霧し、３０～４０℃で３日間２次培養してえられたものを乳酸菌生菌剤とした。得られた乳酸菌生菌剤に含まれる乳酸菌ＢＢ－ＡＣＤ株（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ）の菌数は３．０×１０９ＣＦＵ／ｇであった。以下の実験において、乳酸菌として本実施例にて調製した乳酸菌ＢＢ－ＡＣＤ株を用いた。 (Example 2) Preparation of Lactic Acid Bacteria BB-ACD Strain Bacteria The MRS liquid medium was inoculated with the present bacteria and cultured primarily at 30-40 ° C for 24 hours. The culture solution was sprayed onto a sterilized bran and subjected to secondary culture at 30 to 40 ° C. for 3 days to obtain a lactic acid bacteria viable agent. The number of lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) contained in the obtained lactic acid bacteria viable agent was 3.0 × 10 9 CFU / g. In the following experiments, the lactic acid bacteria BB-ACD strain prepared in this example was used as the lactic acid bacteria.
ＭＲＳ液体培地に本菌を接種し、３０～４０℃で２４時間１次培養した。培養液を滅菌されたふすまに噴霧し、３０～４０℃で３日間２次培養してえられたものを乳酸菌生菌剤とした。得られた乳酸菌生菌剤に含まれる乳酸菌ＢＢ－ＡＣＤ株（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ）の菌数は３．０×１０９ＣＦＵ／ｇであった。以下の実験において、乳酸菌として本実施例にて調製した乳酸菌ＢＢ－ＡＣＤ株を用いた。 (Example 2) Preparation of Lactic Acid Bacteria BB-ACD Strain Bacteria The MRS liquid medium was inoculated with the present bacteria and cultured primarily at 30-40 ° C for 24 hours. The culture solution was sprayed onto a sterilized bran and subjected to secondary culture at 30 to 40 ° C. for 3 days to obtain a lactic acid bacteria viable agent. The number of lactic acid bacteria BB-ACD strain (Lactobacillus acidophilus BB-ACD) contained in the obtained lactic acid bacteria viable agent was 3.0 × 10 9 CFU / g. In the following experiments, the lactic acid bacteria BB-ACD strain prepared in this example was used as the lactic acid bacteria.
（実施例３）肥育牛舎における、乳酸菌生菌剤散布によるプロテウス属の増殖及びアンモニア濃度への作用の検討
初期敷材として麦稈を使用した肥育牛舎に、実施例２で調製した乳酸菌生菌剤を１週間に１回、１００ｇ／ｍ２で散布し、試験開始３０日後に牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない肥育牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した牛舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布３０日目の２回検知管を用いて測定した。 (Example 3) Proliferation of Proteus by spraying live lactic acid bacteria and application to ammonia concentration in fattening cattle barn The live lactobacillus prepared in Example 2 was used in a fattening barn using wheat straw as an initial bedding material. It was sprayed at 100 g / m 2 once a week, and the number of cow bed bacteria (E. coli group, Proteus genus, lactic acid bacteria) was measured 30 days after the start of the test. As a control, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the fattening cow bed not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was sprayed was measured using a detector tube before spraying the lactic acid bacterium and before the 30th day of spraying.
初期敷材として麦稈を使用した肥育牛舎に、実施例２で調製した乳酸菌生菌剤を１週間に１回、１００ｇ／ｍ２で散布し、試験開始３０日後に牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない肥育牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した牛舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布３０日目の２回検知管を用いて測定した。 (Example 3) Proliferation of Proteus by spraying live lactic acid bacteria and application to ammonia concentration in fattening cattle barn The live lactobacillus prepared in Example 2 was used in a fattening barn using wheat straw as an initial bedding material. It was sprayed at 100 g / m 2 once a week, and the number of cow bed bacteria (E. coli group, Proteus genus, lactic acid bacteria) was measured 30 days after the start of the test. As a control, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the fattening cow bed not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was sprayed was measured using a detector tube before spraying the lactic acid bacterium and before the 30th day of spraying.
結果を図１及び図２に示す。乳酸菌散布牛床では、乳酸菌非散布牛床と比較して、乳酸菌数が多く、大腸菌群とプロテウス属の数が少なかった。よって、乳酸菌散布により、牛床における大腸菌群とプロテウス属の増殖が抑制されていることが明らかとなった。また、乳酸菌散布後の牛舎では、乳酸菌散布前の牛舎と比較して牛舎内のアンモニア濃度が低く、乳酸菌散布により、アンモニアの生成が抑制されて悪臭が低減されることが明らかとなった。
The results are shown in FIG. 1 and FIG. The lactic acid bacteria-sprayed cow beds had a larger number of lactic acid bacteria and fewer coliforms and Proteus than the lactic acid bacteria non-sprayed cow beds. Therefore, it was clarified that the growth of coliforms and Proteus in the cattle bed was suppressed by the application of lactic acid bacteria. Moreover, in the barn after lactic acid bacteria application | coating, compared with the cow barn before lactic acid bacteria application | coating, the ammonia density in a cow barn was low, and it became clear that the production | generation of ammonia is suppressed and malodor is reduced by lactic acid bacteria application | coating.
（実施例４）肥育豚舎における、乳酸菌生菌剤散布によるプロテウス属の増殖及びアンモニア濃度への作用の検討
初期敷材としてオガ粉を使用した肥育豚舎に、実施例２で調製した乳酸菌生菌剤を１週間に１回、１００ｇ／ｍ２で散布し、試験開始７日後に豚床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない肥育豚床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した豚舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布７日目の２回検知管を用いて測定した。 (Example 4) Growth of Proteus by spraying live lactic acid bacteria and the effect on ammonia concentration in fattening piggery Lactic acid bacteria live fungus prepared in Example 2 in fattening piggery using sawdust as an initial covering material Was sprayed at 100 g / m 2 once a week, and the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) in the pig bed was measured 7 days after the start of the test. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) in fattening pig beds not sprayed with a lactic acid bacteria viable agent was measured. Moreover, the ammonia concentration in the pig house which sprayed the lactic acid bacteria live bacteria agent was measured using the detection tube twice before the lactic acid bacteria live bacteria drug spraying.
初期敷材としてオガ粉を使用した肥育豚舎に、実施例２で調製した乳酸菌生菌剤を１週間に１回、１００ｇ／ｍ２で散布し、試験開始７日後に豚床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない肥育豚床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した豚舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布７日目の２回検知管を用いて測定した。 (Example 4) Growth of Proteus by spraying live lactic acid bacteria and the effect on ammonia concentration in fattening piggery Lactic acid bacteria live fungus prepared in Example 2 in fattening piggery using sawdust as an initial covering material Was sprayed at 100 g / m 2 once a week, and the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) in the pig bed was measured 7 days after the start of the test. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) in fattening pig beds not sprayed with a lactic acid bacteria viable agent was measured. Moreover, the ammonia concentration in the pig house which sprayed the lactic acid bacteria live bacteria agent was measured using the detection tube twice before the lactic acid bacteria live bacteria drug spraying.
結果を図３及び図４に示す。牛床と同様に、乳酸菌散布豚床では、乳酸菌非散布豚床と比較して、乳酸菌数が多く、大腸菌群とプロテウス属の数が少なかった。よって、乳酸菌散布により、豚床における大腸菌群とプロテウス属の増殖が抑制されていることが明らかとなった。また、乳酸菌散布後の豚舎では、乳酸菌散布前の豚舎と比較して豚舎内のアンモニア濃度が低く、乳酸菌散布により、アンモニアの生成が抑制されて悪臭が低減されることが明らかとなった。
The results are shown in FIGS. Similar to the cattle bed, the lactic acid bacteria-sprayed pigbed had a higher number of lactic acid bacteria and fewer coliforms and Proteus than the lactic acid bacteria-spattered pigbed. Therefore, it was revealed that the growth of coliforms and Proteus in the pig bed was suppressed by the application of lactic acid bacteria. Moreover, in the pig house after lactic acid bacteria application | coating, compared with the pig house before lactic acid bacteria application | coating, the ammonia concentration in a pig house was low, and it became clear that the production | generation of ammonia is suppressed and malodor is reduced by lactic acid bacteria application | coating.
（実施例５）産卵鶏舎における、乳酸菌生菌剤散布によるプロテウス属の増殖及びアンモニア濃度への作用の検討
産卵鶏の平飼鶏舎に、実施例２で調製した乳酸菌生菌剤を１００ｇ／ｍ２で１回散布した。散布から３日後に、鶏舎の床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない鶏舎の床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した鶏舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布３日目の２回検知管を用いて測定した。 (Example 5) Examination of Proteus genus growth and ammonia concentration effect by spraying live lactic acid bacteria in laying hen house 100 g / m 2 of lactic acid bacterium prepared in Example 2 in flat breeding house of laying hen Sprayed once. Three days after spraying, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the poultry house floor was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the poultry house floor that was not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the poultry house sprayed with the lactic acid bacteria live bacteria was measured using a detector tube before spraying the lactic acid bacteria live bacteria and on the third day of spraying.
産卵鶏の平飼鶏舎に、実施例２で調製した乳酸菌生菌剤を１００ｇ／ｍ２で１回散布した。散布から３日後に、鶏舎の床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を散布していない鶏舎の床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を散布した鶏舎内のアンモニア濃度を、乳酸菌生菌剤散布前と散布３日目の２回検知管を用いて測定した。 (Example 5) Examination of Proteus genus growth and ammonia concentration effect by spraying live lactic acid bacteria in laying hen house 100 g / m 2 of lactic acid bacterium prepared in Example 2 in flat breeding house of laying hen Sprayed once. Three days after spraying, the number of bacteria (Coliform group, Proteus genus, lactic acid bacteria) on the poultry house floor was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the poultry house floor that was not sprayed with the lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the poultry house sprayed with the lactic acid bacteria live bacteria was measured using a detector tube before spraying the lactic acid bacteria live bacteria and on the third day of spraying.
結果を図５及び図６に示す。牛床や豚床と同様に、乳酸菌散布鶏舎の床では、乳酸菌非散布鶏舎の床と比較して、乳酸菌数が多く、大腸菌群とプロテウス属の数が少なかった。よって、乳酸菌散布により、鶏舎の床における大腸菌群とプロテウス属の増殖が抑制されていることが明らかとなった。また、乳酸菌散布後の鶏舎では、乳酸菌散布前の鶏舎と比較して鶏舎内のアンモニア濃度が低く、乳酸菌散布により、アンモニアの生成が抑制されて悪臭が低減されることが明らかとなった。
The results are shown in FIGS. Similar to the cow and pig beds, the number of lactic acid bacteria and the number of genus Proteus were lower in the lactic acid bacteria-sprayed chicken house floors than in the lactic acid bacteria-free chicken house floors. Therefore, it was revealed that the growth of coliforms and Proteus on the poultry house floor was suppressed by the application of lactic acid bacteria. Moreover, in the poultry house after the lactic acid bacteria application, the ammonia concentration in the poultry house was lower than the hen house before the lactic acid bacteria application, and it became clear that the production of ammonia was suppressed and the malodor was reduced by the lactic acid bacteria application.
以上より、家畜の種類を問わず、乳酸菌ＢＢ－ＡＣＤ株生菌剤を散布することにより畜舎の床における大腸菌群、及びプロテウス属の増殖が抑制されると共に、畜舎内でのアンモニアの生成が抑制されて悪臭が低減することが示された。
From the above, regardless of the type of livestock, the growth of Escherichia coli and Proteus on the floor of the barn is suppressed and the production of ammonia in the barn is suppressed by spraying the lactic acid bacteria BB-ACD strain. Has been shown to reduce malodor.
（実施例６）肥育牛に乳酸菌生菌剤を摂取させることによるプロテウス属の増殖及びアンモニア濃度への作用の検討
交雑種肥育牛に、１頭あたり１日４０ｇの乳酸菌製剤を通常飼料に混合して１４日間給与し、給与前後における牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を給与していない肥育牛を飼育している床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を給与した牛舎内のアンモニア濃度を、乳酸菌生菌剤給与前と散布１４日目の２回検知管を用いて測定した。 (Example 6) Examination of the effect of Proteus genus growth and ammonia concentration by feeding fattening cattle with a lactic acid bacteria viable agent 40 g of lactic acid bacteria preparation per day is mixed with normal feed to hybrid breeding cattle For 14 days, and the number of bacteria (coliform group, Proteus, lactic acid bacteria) in the cow bed before and after feeding was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the floor that raised fattening cows that were not fed with a lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was fed was measured using a detection tube twice before feeding the lactic bacterium and 14 days after spraying.
交雑種肥育牛に、１頭あたり１日４０ｇの乳酸菌製剤を通常飼料に混合して１４日間給与し、給与前後における牛床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。コントロールとして、乳酸菌生菌剤を給与していない肥育牛を飼育している床の細菌（大腸菌群、プロテウス属、乳酸菌）の数を測定した。また、乳酸菌生菌剤を給与した牛舎内のアンモニア濃度を、乳酸菌生菌剤給与前と散布１４日目の２回検知管を用いて測定した。 (Example 6) Examination of the effect of Proteus genus growth and ammonia concentration by feeding fattening cattle with a lactic acid bacteria viable agent 40 g of lactic acid bacteria preparation per day is mixed with normal feed to hybrid breeding cattle For 14 days, and the number of bacteria (coliform group, Proteus, lactic acid bacteria) in the cow bed before and after feeding was measured. As a control, the number of bacteria (E. coli group, Proteus genus, lactic acid bacteria) on the floor that raised fattening cows that were not fed with a lactic acid bacteria viable agent was measured. In addition, the ammonia concentration in the barn where the lactic acid bacterium was fed was measured using a detection tube twice before feeding the lactic bacterium and 14 days after spraying.
結果を図７及び図８に示す。散布と同様に、乳酸菌給与牛舎の床では、乳酸菌非給与牛舎の床と比較して、乳酸菌数が多く、大腸菌群とプロテウス属の数が少なかった。よって、乳酸菌ＢＢ－ＡＣＤ株を給与させることにより、牛舎の床における大腸菌群とプロテウス属の増殖が抑制されていることが明らかとなった。また、乳酸菌給与後の牛舎では、乳酸菌給与前の牛舎と比較して牛舎内のアンモニア濃度が低く、乳酸菌給与により、アンモニアの生成が抑制されて悪臭が低減されることが明らかとなった。
The results are shown in FIGS. As with spraying, the number of lactic acid bacteria was higher and the number of coliforms and Proteus was lower in the dairy barn floor than in the non-lactic barn floor. Therefore, it was clarified that feeding the lactic acid bacteria BB-ACD strain suppressed the growth of coliforms and Proteus in the cowshed floor. Moreover, in the barn after feeding lactic acid bacteria, the ammonia concentration in the barn was lower than in the barn before feeding lactic acid bacteria, and it became clear that the generation of ammonia was suppressed and malodor was reduced by feeding lactic acid bacteria.
よって、乳酸菌ＢＢ－ＡＣＤ株は散布のみならず、給与することによっても、畜舎の床における大腸菌群、及びプロテウス属の増殖が抑制すると共に、畜舎内でのアンモニアの生成を抑制して悪臭が低減することが示された。
Therefore, the lactic acid bacteria BB-ACD strain is not only sprayed, but also when fed, it suppresses the growth of coliforms and Proteus on the barn floor and suppresses the production of ammonia in the barn to reduce malodor Was shown to do.
（実施例７）Ｐｒｏｔｅｕｓ属に対する抗菌活性
酵母エキス含有脱脂粉乳溶液（脱脂粉乳１０．０ｇと酵母エキス０．５ｇを蒸留水１００ｍｌに溶解し、１１０℃、２０分間高圧滅菌）に、ＭＲＳブロスを用いて培養した本菌を１ｍｌ接種し、３０～４０℃で３～５日間培養した。抗菌活性の測定には、１４，５００ｇ、１０分間の遠心分離処理をして得られた上清を、孔径０．２μｍのフィルターでろ過した除菌清澄液を用いたアガーウェル法で検討した。なお、被検菌は、Ｐｒｏｔｅｕｓ ｍｉｒａｂｉｌｉｓ ＮＢＲＣ１０５６９７を用いた。 (Example 7) Antibacterial activity against the genus Proteus Using MRS broth in a yeast extract-containing skim milk solution (10.0 g of skim milk powder and 0.5 g of yeast extract dissolved in 100 ml of distilled water and sterilized at 110 ° C. for 20 minutes) 1 ml of the cultured bacterium was inoculated and cultured at 30 to 40 ° C. for 3 to 5 days. For the measurement of the antibacterial activity, the supernatant obtained by centrifuging for 14,500 g for 10 minutes was examined by an agarwell method using a sterilized clarified liquid filtered through a filter having a pore size of 0.2 μm. In addition, Proteus mirabilis NBRC105697 was used for the test bacteria.
酵母エキス含有脱脂粉乳溶液（脱脂粉乳１０．０ｇと酵母エキス０．５ｇを蒸留水１００ｍｌに溶解し、１１０℃、２０分間高圧滅菌）に、ＭＲＳブロスを用いて培養した本菌を１ｍｌ接種し、３０～４０℃で３～５日間培養した。抗菌活性の測定には、１４，５００ｇ、１０分間の遠心分離処理をして得られた上清を、孔径０．２μｍのフィルターでろ過した除菌清澄液を用いたアガーウェル法で検討した。なお、被検菌は、Ｐｒｏｔｅｕｓ ｍｉｒａｂｉｌｉｓ ＮＢＲＣ１０５６９７を用いた。 (Example 7) Antibacterial activity against the genus Proteus Using MRS broth in a yeast extract-containing skim milk solution (10.0 g of skim milk powder and 0.5 g of yeast extract dissolved in 100 ml of distilled water and sterilized at 110 ° C. for 20 minutes) 1 ml of the cultured bacterium was inoculated and cultured at 30 to 40 ° C. for 3 to 5 days. For the measurement of the antibacterial activity, the supernatant obtained by centrifuging for 14,500 g for 10 minutes was examined by an agarwell method using a sterilized clarified liquid filtered through a filter having a pore size of 0.2 μm. In addition, Proteus mirabilis NBRC105697 was used for the test bacteria.
結果を図９に示す。その結果、阻止円の形成が認められた。
The results are shown in FIG. As a result, formation of a blocking circle was recognized.
The results are shown in FIG. As a result, formation of a blocking circle was recognized.
Claims (15)
- Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ ＢＢ－ＡＣＤ株（ＮＩＴＥ ＢＰ－０２０９８）。 Lactobacillus acidophilus BB-ACD strain (NITE BP-02098).
- 請求項１に記載の乳酸菌を含有する、有害菌増殖抑制剤。 A harmful fungus growth inhibitor containing the lactic acid bacterium according to claim 1.
- 前記有害菌が、大腸菌及び／又はプロテウス属である、請求項２に記載の有害菌増殖抑制剤。 The harmful bacteria growth inhibitor according to claim 2, wherein the harmful bacteria are Escherichia coli and / or Proteus.
- 散布用製剤又は家畜給与用製剤である、請求項２又は請求項３に記載の有害菌増殖抑制剤。 4. The harmful bacteria growth inhibitor according to claim 2 or 3, which is a spray preparation or a livestock feed preparation.
- 請求項１に記載の乳酸菌を含有する、家畜用飼料。 A livestock feed comprising the lactic acid bacterium according to claim 1.
- 請求項１に記載の乳酸菌を散布することを備える、有害菌増殖抑制方法。 A method for inhibiting the growth of harmful bacteria, comprising spraying the lactic acid bacteria according to claim 1.
- 請求項１に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の床における有害菌増殖抑制方法。 A method for inhibiting the growth of harmful bacteria on a floor in a barn, comprising spraying the lactic acid bacteria according to claim 1 on the floor in a barn.
- 請求項１に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における有害菌増殖を抑制する方法。 A method for suppressing the growth of harmful bacteria in a barn, comprising feeding the lactic acid bacteria according to claim 1 to livestock animals.
- 家畜動物が牛である、請求項８に記載の方法。 The method according to claim 8, wherein the livestock animal is a cow.
- 前記有害菌が、大腸菌及び／又はプロテウス属である、請求項６～請求項９のいずれか１項に記載の方法。 The method according to any one of claims 6 to 9, wherein the harmful bacteria are Escherichia coli and / or Proteus.
- 請求項１に記載の乳酸菌を散布することを備える、悪臭低減方法。 A malodor reduction method comprising spraying the lactic acid bacteria according to claim 1.
- 請求項１に記載の乳酸菌を畜舎内の床に散布することを備える、畜舎内の悪臭低減方法。 A method for reducing malodor in a barn, comprising spraying the lactic acid bacteria according to claim 1 on a floor in the barn.
- 請求項１に記載の乳酸菌を家畜動物に給与することを備える、畜舎内における悪臭低減方法。 A method for reducing malodor in a barn comprising feeding the lactic acid bacteria according to claim 1 to a livestock animal.
- 家畜動物が牛である、請求項１３に記載の方法。 The method according to claim 13, wherein the livestock animal is a cow.
- 悪臭が、アンモニアによる悪臭である、請求項１１～請求項１４のいずれか１項に記載の方法。 The method according to any one of claims 11 to 14, wherein the malodor is a malodor due to ammonia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017537790A JP6864293B2 (en) | 2015-08-31 | 2016-08-25 | Foul odor reduction method |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015170468 | 2015-08-31 | ||
| JP2015-170468 | 2015-08-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2017038602A1 true WO2017038602A1 (en) | 2017-03-09 |
Family
ID=58187309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2016/074748 WO2017038602A1 (en) | 2015-08-31 | 2016-08-25 | Odour reduction method |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP6864293B2 (en) |
| WO (1) | WO2017038602A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108546661A (en) * | 2018-04-26 | 2018-09-18 | 湖北华大瑞尔科技有限公司 | A kind of antibacterial type microbe leaven and preparation method thereof |
| CN112335556A (en) * | 2020-10-19 | 2021-02-09 | 广州动物园 | Novel captive wild animal management method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08298982A (en) * | 1995-05-02 | 1996-11-19 | Aasu Giken:Kk | Complex microbial pharmaceutical preparation |
| JPH09252728A (en) * | 1996-03-25 | 1997-09-30 | Natl Fedelation Of Agricult Coop Assoc | Deodorizing feed |
| JP2001299230A (en) * | 2000-04-27 | 2001-10-30 | Korin Korea Co Ltd | Feed additive and method for producing the same |
| JP2005065543A (en) * | 2003-08-21 | 2005-03-17 | Oubiken:Kk | Livestock-rearing floor and method for rearing livestock |
| JP2005245299A (en) * | 2004-03-04 | 2005-09-15 | Bio Balance:Kk | New lactic acid bacterium |
| KR100654427B1 (en) * | 2005-09-23 | 2006-12-06 | 심명식 | Lactobacillus plantarum cu03 kacc 91103 having provention of formating a foul odor and a deodorizing acitivy |
| JP2008000118A (en) * | 2006-06-20 | 2008-01-10 | Fujika Sogyo:Kk | Method for sterilizing livestock rearing farm |
| JP2009057284A (en) * | 2005-12-21 | 2009-03-19 | Saihatsu Ko | Microbial preparation for reducing greenhouse effect gas excreted from livestock or fowl |
-
2016
- 2016-08-25 WO PCT/JP2016/074748 patent/WO2017038602A1/en active Application Filing
- 2016-08-25 JP JP2017537790A patent/JP6864293B2/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08298982A (en) * | 1995-05-02 | 1996-11-19 | Aasu Giken:Kk | Complex microbial pharmaceutical preparation |
| JPH09252728A (en) * | 1996-03-25 | 1997-09-30 | Natl Fedelation Of Agricult Coop Assoc | Deodorizing feed |
| JP2001299230A (en) * | 2000-04-27 | 2001-10-30 | Korin Korea Co Ltd | Feed additive and method for producing the same |
| JP2005065543A (en) * | 2003-08-21 | 2005-03-17 | Oubiken:Kk | Livestock-rearing floor and method for rearing livestock |
| JP2005245299A (en) * | 2004-03-04 | 2005-09-15 | Bio Balance:Kk | New lactic acid bacterium |
| KR100654427B1 (en) * | 2005-09-23 | 2006-12-06 | 심명식 | Lactobacillus plantarum cu03 kacc 91103 having provention of formating a foul odor and a deodorizing acitivy |
| JP2009057284A (en) * | 2005-12-21 | 2009-03-19 | Saihatsu Ko | Microbial preparation for reducing greenhouse effect gas excreted from livestock or fowl |
| JP2008000118A (en) * | 2006-06-20 | 2008-01-10 | Fujika Sogyo:Kk | Method for sterilizing livestock rearing farm |
Non-Patent Citations (1)
| Title |
|---|
| CHIHARU WATANABE ET AL.: "Nyusankin Shizai no Shiryo Tenka ga Keifun no Haisetsuryo to Shuki ni Oyobosu Eikyo", BULLETIN OF THE SHIGA PREFECTURAL LIVESTOCK RESEARCH AND IMPROVEMENT INSTITUTE, vol. 6, 1999, pages 34 - 39 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108546661A (en) * | 2018-04-26 | 2018-09-18 | 湖北华大瑞尔科技有限公司 | A kind of antibacterial type microbe leaven and preparation method thereof |
| CN108546661B (en) * | 2018-04-26 | 2021-10-08 | 湖北华大瑞尔科技有限公司 | Application method and preparation method of bacteriostatic microbial starter |
| CN112335556A (en) * | 2020-10-19 | 2021-02-09 | 广州动物园 | Novel captive wild animal management method |
| CN112335556B (en) * | 2020-10-19 | 2022-05-06 | 广州动物园 | Novel captive wild animal management method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2017038602A1 (en) | 2018-08-30 |
| JP6864293B2 (en) | 2021-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2372788C2 (en) | Probiotic, health- or productivity-stimulating fodder additive or portable water additive and its application | |
| Koshchaev et al. | Screening of microorganism symbiont strains as a base of probiotics for poultry industry | |
| AU2013322644B2 (en) | Probiotic and prebiotic compositions | |
| CN107312726A (en) | One lactobacillus plantarum ZN 3 and application | |
| US20150104418A1 (en) | Bacterial composition | |
| KR101854277B1 (en) | Microbial agent comprising a mixture of species for reducing ammonia odor and method for manufacturing the same | |
| JP2009057284A (en) | Microbial preparation for reducing greenhouse effect gas excreted from livestock or fowl | |
| KR100949903B1 (en) | Microorganisms preparations for the additional feedstuff and preparation thereof | |
| CN111826323A (en) | Bacillus subtilis, preparation and application thereof | |
| JP4199685B2 (en) | New lactic acid bacteria | |
| WO2017038602A1 (en) | Odour reduction method | |
| US20110123504A1 (en) | Method to reduce pathogenic bactgeriae in a stable for farm animals | |
| KR101212031B1 (en) | Novel microorganism candida glabrata with effect reducing odor of livestock waste, probiotic composite for domestic animal feed including the same, and method for manufacturing therefof | |
| US20150257391A1 (en) | Bacillus licheniformis strain | |
| JP2006169197A5 (en) | ||
| Piskaeva et al. | Comparative analysis of the activity of silver nanoparticles against native microflora from poultry processing plants wastes | |
| KR102657429B1 (en) | Novel Lactobacillus plantarum strain having malodor reducing activity and Use thereof | |
| RU2409939C2 (en) | Incubatory chicken eggs sanation method | |
| CN101081295A (en) | Antibiotic, composition containg antibiotic and method for the administration of antibiotic and said composition to livestock | |
| US9724372B2 (en) | Calf administered bacterial composition | |
| US9724371B2 (en) | Feedlot administered bacterial composition | |
| JPH09163938A (en) | Blcs poutry farming sterilization | |
| JP2007159563A (en) | Feed additive containing bacillus thuringiensis | |
| WO2018155612A1 (en) | Composition for prevention and/or treatment of infection relating to salmonella including bacillus amyloliquefaciens strain and/or treated product of said strain | |
| RU2662931C1 (en) | Biological base of microbial fodder additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 16841634 Country of ref document: EP Kind code of ref document: A1 |
|
| ENP | Entry into the national phase |
Ref document number: 2017537790 Country of ref document: JP Kind code of ref document: A |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 16841634 Country of ref document: EP Kind code of ref document: A1 |