WO2017035882A1 - 一种尿液氨基酸代谢物检测试剂的制备方法 - Google Patents

一种尿液氨基酸代谢物检测试剂的制备方法 Download PDF

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WO2017035882A1
WO2017035882A1 PCT/CN2015/090538 CN2015090538W WO2017035882A1 WO 2017035882 A1 WO2017035882 A1 WO 2017035882A1 CN 2015090538 W CN2015090538 W CN 2015090538W WO 2017035882 A1 WO2017035882 A1 WO 2017035882A1
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preparing
amino acid
copper
acid metabolite
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扈晓佳
梅宗泉
胡梦丽
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苏州锐霖生物科技有限公司
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity

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  • the invention relates to the field of detection reagents, in particular to a preparation method of a urine amino acid metabolite detection reagent.
  • the invention aims at the deficiencies of the prior art, and provides a preparation method of a urine amino acid metabolite detecting reagent with high sensitivity, simple operation, high accuracy and low cost.
  • the invention adopts the following technical scheme: a preparation method of a urine amino acid metabolite detecting reagent, which comprises the following steps:
  • the preparation method of the aforementioned urine amino acid metabolite detecting reagent, the temperature of the mixture of the A solution, the B solution, the C solution, the D solution, and the A solution, the B solution, the C solution, and the D solution is between 15 and 20 degrees Celsius.
  • the preparation method of the urine amino acid metabolite detecting reagent described above, the preparation process of the A solution is as follows: 20-25 g copper is dissolved in 250 ml of 0.5-1 M nitrous acid solution, and after the copper is completely dissolved, it is prepared. A solution.
  • the preparation method of the urine amino acid metabolite detecting reagent described above, the preparation process of the B solution is as follows: 10-25 g of copper is dissolved in 250 ml of a 2-3 M nitric acid solution, and after the copper is completely dissolved, the B is prepared. Solution.
  • the preparation method of the aforementioned urine amino acid metabolite detecting reagent, the preparation process of the C solution is as follows: 15-25 g of mercury is dissolved in 250 ml of a 2-3 M sulfuric acid solution, and after the mercury is completely dissolved, a C solution is prepared.
  • the preparation method of the urine amino acid metabolite detecting reagent described above, the preparation process of the D solution is as follows: 15-25 g of aluminum is dissolved in 250 ml of a 2-3 M sulfuric acid solution, and after the aluminum is completely dissolved, the D is prepared. Solution.
  • the A solution, the B solution, the C solution, and the D solution are mixed at a mass ratio of 1:1:1:1.
  • the aforementioned method for preparing a urine amino acid metabolite detecting reagent wherein the mixture of ammonium persulfate and sodium hydrogen sulfite accounts for 1-2% by mass of the urine amino acid metabolite detecting reagent; the quality of ammonium persulfate and sodium hydrogen sulfite The ratio is 1:1.
  • the invention adopts the A solution, the B solution, the C solution and the D solution to mix at the optimal mass ratio and the optimal concentration and adds a mixture of ammonium persulfate and sodium hydrogen sulfite to prepare a detection reagent with high sensitivity, false positive rate and The false negative rate is low, and the operation is simple and low cost; the nitrous acid concentration is low and the preparation temperature is also low, so that the nitrite is more stable, thereby improving the detection accuracy and sensitivity.
  • a nickel nitrate solution was prepared as a B solution; 25 g of copper was dissolved in 250 ml of a 2 M nitric acid solution, and after the copper was completely dissolved, a B solution was prepared.
  • a mercury nitrate solution was prepared as a C solution; 25 g of iron was dissolved in 250 ml of a 2 M nitric acid solution, and after the iron was completely dissolved, a C solution was prepared.
  • the temperature of the mixture of the A solution, the B solution, the C solution, and the A solution, the B solution, and the C solution is between 15 and 20 degrees Celsius.
  • the detection method is as follows: taking 3 ml of urine of the subject, dropping into 0.5 ml of detection reagent, shaking and standing for 3 min, the detection standard is: white/yellow precipitate or no precipitation, negative, gray/black
  • the precipitater is positive, and the color palette can be set for different colors from gray to black. The deeper the color, the more the amino acid metabolite content, the more serious the tumor may be.
  • the color level 0 is a negative result, and the remaining color levels are gray or black.
  • the detection rate of the detection reagent of the present invention for liver cancer patients is about 95%, which is higher than the existing detection reagents.
  • the detection reagent of the present invention does not cause false positives, and is superior to the existing detection reagents.

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Abstract

一种尿液氨基酸代谢物检测试剂的制备方法，包括如下步骤：制备0.2-1g/ml的亚硝酸铜溶液，作为A溶液；制备0.1-1g/ml的硝酸铜溶液，作为B溶液；制备0.15-1g/ml的硫酸汞溶液，作为C溶液；制备0.15-1g/ml的硫酸铝溶液，作为D溶液；将A溶液、B溶液、C溶液、D溶液以质量比为1：(0.5-1)：(0.5-1)：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。

Description

一种尿液氨基酸代谢物检测试剂的制备方法 技术领域
本发明涉及检测试剂领域，具体涉及一种尿液氨基酸代谢物检测试剂的制备方法。
背景技术
恶性肿瘤是世界性疾病，也是人类最凶恶的杀手之一，全世界每年死于癌症的人数为800万左右。多年来，国内外许多专家学者对恶性肿瘤的早期发现和诊断进行了多方面的研究探讨，多种肿瘤标志物相继应用于对癌症病人的临床诊断并取得了一定的效果，但它们存在着操作繁琐、成本投入高、准确度易受多种因素影响等缺点。并且这些方法大多都是有创性的，这对于疾病的大规模筛查不利。因此，建立一种快速的无创肿瘤筛查方法更能适应大规模人群的健康筛查。随着代谢组学的兴起，已从寻找单一生物标志物转向寻找特异性的一组标志物群，从而开创肿瘤诊断方法研究的新途径。
发明内容
本发明针对现有技术的不足，提供了一种灵敏度高、操作简单、准确度高、成本较低的尿液氨基酸代谢物检测试剂的制备方法。
本发明采用以下技术方案：一种尿液氨基酸代谢物检测试剂的制备方法，其特征在于，包括如下步骤：
(1)制备0.2-1g/ml的亚硝酸铜溶液，作为A溶液；
(2)制备0.1-1g/ml的硝酸铜溶液，作为B溶液；
(3)制备0.15-1g/ml的硫酸汞溶液，作为C溶液；
(4)制备0.15-1g/ml的硫酸铝溶液，作为D溶液；
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:(0.5-1):(0.5-1)：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。
前述的尿液氨基酸代谢物检测试剂的制备方法，A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度之间。
前述的尿液氨基酸代谢物检测试剂的制备方法，所述的A溶液的制备过程如下：将20--25g铜溶于250ml的0.5-1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液。
前述的尿液氨基酸代谢物检测试剂的制备方法，所述的B溶液的制备过程如下：将10--25g铜溶于250ml的2-3M的硝酸溶液中，待铜完全溶解后，制成B溶液。
前述的尿液氨基酸代谢物检测试剂的制备方法，所述的C溶液的制备过程如下：将 15--25g汞溶于250ml的2-3M的硫酸溶液中，待汞完全溶解后，制成C溶液。
前述的尿液氨基酸代谢物检测试剂的制备方法，所述的D溶液的制备过程如下：将15--25g铝溶于250ml的2-3M的硫酸溶液中，待铝完全溶解后，制成D溶液。
前述的尿液氨基酸代谢物检测试剂的制备方法，在步骤(5)中，A溶液、B溶液、C溶液、D溶液以质量比为1:1:1：1混合。
前述的尿液氨基酸代谢物检测试剂的制备方法，过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为1-2％；过硫酸铵和亚硫酸氢钠的质量比为1:1。
本发明采用A溶液、B溶液、C溶液、D溶液以最优质量比和最佳浓度混合并且加上过硫酸铵和亚硫酸氢钠的混合物以制成检测试剂，灵敏度高，假阳性率和假阴性率低，且操作简单成本低；采用的亚硝酸浓度较低且配制温度也较低，以使得亚硝酸盐更为稳定，进而提高检测准确度和灵敏度。
具体实施方式
下面结合具体实施例对本发明作进一步详细介绍。
制备实施例1
(1)制备亚硝酸铜溶液，作为A溶液；将20g铜溶于250ml的1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液；
(2)制备硝酸铜溶液，作为B溶液；将20g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液；
(3)制备硫酸汞溶液，作为C溶液；将20g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成C溶液；
(4)制备硫酸铝溶液，作为D溶液；将20g铝溶于250ml的2M的硫酸溶液中，待铝完全溶解后，制成D溶液。
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:1:1：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为1％；过硫酸铵和亚硫酸氢钠的质量比为1:1。A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度之间。
制备实施例2
(1)制备亚硝酸铜溶液，作为A溶液；将20g铜溶于250ml的1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液；
(2)制备硝酸铜溶液，作为B溶液；将15g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液；
(3)制备硫酸汞溶液，作为C溶液；将15g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成C溶液；
(4)制备硫酸铝溶液，作为D溶液；将15g铝溶于250ml的2M的硫酸溶液中，待铝完全溶解后，制成D溶液。
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:0.5:0.5：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为2％；过硫酸铵和亚硫酸氢钠的质量比为1:1。A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度之间。
制备实施例3
(1)制备亚硝酸铜溶液，作为A溶液；将20g铜溶于250ml的1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液；
(2)制备硝酸铜溶液，作为B溶液；将25g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液；
(3)制备硫酸汞溶液，作为C溶液；将25g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成C溶液；
(4)制备硫酸铝溶液，作为D溶液；将25g铝溶于250ml的2M的硫酸溶液中，待铝完全溶解后，制成D溶液。
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:0.5:0.5：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为1.5％；过硫酸铵和亚硫酸氢钠的质量比为1:1。A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度之间。
对比例1
(1)制备硫酸汞溶液，作为A溶液；将15g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成A溶液；
(2)制备硝酸镍溶液，作为B溶液；将25g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液。
(3)制备硝酸汞溶液，作为C溶液；将25g铁溶于250ml的2M的硝酸溶液中，待铁完全溶解后，制成C溶液。
(4)将A溶液、B溶液、C溶液以质量比为1:1:1：1混合后，加入防腐剂，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂；
A溶液、B溶液、C溶液以及A溶液、B溶液、C溶液的混合液的温度均为15-20摄氏度之间。
对比例2
(1)制备亚硝酸铜溶液，作为A溶液；将20g铜溶于250ml的1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液；
(2)制备硝酸铜溶液，作为B溶液；将25g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液；
(3)制备硫酸汞溶液，作为C溶液；将25g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成C溶液；
(4)制备硫酸铝溶液，作为D溶液；将25g铝溶于250ml的2M的硫酸溶液中，待铝完全溶解后，制成D溶液。
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:0.5:0.5：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为1.5％；过硫酸铵和亚硫酸氢钠的质量比为1:1。A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为30-35摄氏度。
对比例3
(1)制备亚硝酸铜溶液，作为A溶液；将20g铜溶于250ml的1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液；
(2)制备硝酸铜溶液，作为B溶液；将25g铜溶于250ml的2M的硝酸溶液中，待铜完全溶解后，制成B溶液；
(3)制备硫酸汞溶液，作为C溶液；将25g汞溶于250ml的2M的硫酸溶液中，待汞完全溶解后，制成C溶液；
(4)制备硫酸铝溶液，作为D溶液；将25g铝溶于250ml的2M的硫酸溶液中，待铝完全溶解后，制成D溶液。
(5)将A溶液、B溶液、C溶液、D溶液以质量比为1:0.5:0.5：1混合后，搅拌均匀并制成 淡绿色的尿液氨基酸代谢物检测试剂。A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度。
取100个确诊的肝癌病人尿液样本、以及10个无癌症样本(即阴性样本)，分别用制备实施例1-3和对比例1-3进行尿液氨基酸代谢物检测，检测结果如下表1和表2。
检测方法为：取被检者尿液3ml，滴入0.5ml的检测试剂中，摇匀，静置3min，检测标准是：出现白色/黄色沉淀或者不出现沉淀者，为阴性，出现灰色/黑色沉淀者为阳性，对由灰色到黑色的不同颜色可以设置比色板，颜色越深说明氨基酸代谢物含量越多，肿瘤可能越严重。其中颜色等级0为阴性结果，其余颜色等级为灰色或黑色。
表1对肝癌病人的检测结果
Figure PCTCN2015090538-appb-000001
表2对阴性样本的检测结果
Figure PCTCN2015090538-appb-000002
由表1可知，本发明的检测试剂对肝癌病人的检出率为95％左右，高于现有的检测试剂。由表2可知，本发明的检测试剂不会造成假阳性，也优于现有的检测试剂。
需要说明的是，以上所述仅为本发明的实施例，并非因此限制本发明的专利范围，凡是利用本发明说明书及附图内容所做的等效结构或等效流程变换，或直接或间接运用在其他相关技术领域，均同理包括在本发明的专利保护范围内。

Claims (8)

  1. 一种尿液氨基酸代谢物检测试剂的制备方法，其特征在于，包括如下步骤：
    (1)制备0.2-1g/ml的亚硝酸铜溶液，作为A溶液；
    (2)制备0.1-1g/ml的硝酸铜溶液，作为B溶液；
    (3)制备0.15-1g/ml的硫酸汞溶液，作为C溶液；
    (4)制备0.15-1g/ml的硫酸铝溶液，作为D溶液；
    (5)将A溶液、B溶液、C溶液、D溶液以质量比为1:(0.5-1):(0.5-1)：1混合后，加入过硫酸铵和亚硫酸氢钠的混合物，搅拌均匀并制成淡绿色的尿液氨基酸代谢物检测试剂。
  2. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，A溶液、B溶液、C溶液、D溶液以及A溶液、B溶液、C溶液和D溶液的混合液的温度均为15-20摄氏度之间。
  3. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，所述的A溶液的制备过程如下：将20--25g铜溶于250ml的0.5-1M的亚硝酸溶液中，待铜完全溶解后，制成A溶液。
  4. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，所述的B溶液的制备过程如下：将10--25g铜溶于250ml的2-3M的硝酸溶液中，待铜完全溶解后，制成B溶液。
  5. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，所述的C溶液的制备过程如下：将15--25g汞溶于250ml的2-3M的硫酸溶液中，待汞完全溶解后，制成C溶液。
  6. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，所述的D溶液的制备过程如下：将15--25g铝溶于250ml的2-3M的硫酸溶液中，待铝完全溶解后，制成D溶液。
  7. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，在步骤(5)中，A溶液、B溶液、C溶液、D溶液以质量比为1:1:1：1混合。
  8. 根据权利要求1所述的尿液氨基酸代谢物检测试剂的制备方法，其特征在于，过硫酸铵和亚硫酸氢钠的混合物占尿液氨基酸代谢物检测试剂的质量百分比为1-2％；过硫酸铵和亚硫酸氢钠的质量比为1:1。
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3917401A4 (en) * 2019-01-28 2022-10-12 IOI Auranae Kft. DEVICE AND METHOD FOR AUTOMATIC DETERMINATION OF ANION AND CATION LEVELS FROM URINE

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1189619A (zh) * 1997-01-30 1998-08-05 傅敏恭 癌症尿检比色方法及其装置
CN102368069A (zh) * 2011-03-25 2012-03-07 吉林生物研究院有限公司 对羟基苯丙氨酸尿液检测试剂及其制备方法
CN104390975A (zh) * 2014-10-31 2015-03-04 江苏东博生物医药有限公司 尿液中肿瘤标志物5-羟吲哚乙酸检测试剂及制备方法
CN104535565A (zh) * 2014-12-25 2015-04-22 江苏东博生物医药有限公司 单羟酚类代谢物尿液检测试剂及其制备方法
CN104597046A (zh) * 2015-01-21 2015-05-06 重庆都好生物科技有限公司 癌症尿液检测试剂

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1189619A (zh) * 1997-01-30 1998-08-05 傅敏恭 癌症尿检比色方法及其装置
CN102368069A (zh) * 2011-03-25 2012-03-07 吉林生物研究院有限公司 对羟基苯丙氨酸尿液检测试剂及其制备方法
CN104390975A (zh) * 2014-10-31 2015-03-04 江苏东博生物医药有限公司 尿液中肿瘤标志物5-羟吲哚乙酸检测试剂及制备方法
CN104535565A (zh) * 2014-12-25 2015-04-22 江苏东博生物医药有限公司 单羟酚类代谢物尿液检测试剂及其制备方法
CN104597046A (zh) * 2015-01-21 2015-05-06 重庆都好生物科技有限公司 癌症尿液检测试剂

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3917401A4 (en) * 2019-01-28 2022-10-12 IOI Auranae Kft. DEVICE AND METHOD FOR AUTOMATIC DETERMINATION OF ANION AND CATION LEVELS FROM URINE

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