WO2016206654A3 - 一种碱性磷酸酶的酶促化学发光底物 - Google Patents
一种碱性磷酸酶的酶促化学发光底物 Download PDFInfo
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- WO2016206654A3 WO2016206654A3 PCT/CN2016/096755 CN2016096755W WO2016206654A3 WO 2016206654 A3 WO2016206654 A3 WO 2016206654A3 CN 2016096755 W CN2016096755 W CN 2016096755W WO 2016206654 A3 WO2016206654 A3 WO 2016206654A3
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- chemiluminescent substrate
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- alkaline phosphatase
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
一种碱性磷酸酶的酶促化学发光底物,该酶促化学发光底物以水为溶剂,且还包括以下组分:2-氨基-2-甲基-1-丙醇;AMPPD;以及脂肪醇聚氧乙烯醚羧酸钠与荧光化合物偶联的发光增强剂。该发光增强剂可起到共表面活性剂的作用,能使该底物更好的结合到化学发光缓冲体系中,从而大幅度提高化学发光效率。该酶促化学发光底物具有强度高,灵敏度高,持续时间长,稳定性好等优点,可完全满足临床检测的需要。
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CN201510359183.0A CN104990912B (zh) | 2015-06-26 | 2015-06-26 | 一种碱性磷酸酶的酶促化学发光底物 |
CN201510359183.0 | 2015-06-26 |
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WO2016206654A2 WO2016206654A2 (zh) | 2016-12-29 |
WO2016206654A3 true WO2016206654A3 (zh) | 2017-02-09 |
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Families Citing this family (21)
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CN104990912B (zh) * | 2015-06-26 | 2018-04-20 | 江苏浩欧博生物医药股份有限公司 | 一种碱性磷酸酶的酶促化学发光底物 |
CN105259173B (zh) * | 2015-11-02 | 2018-01-30 | 迈克生物股份有限公司 | 一种组合试剂及其应用及含有该组合试剂的试剂盒 |
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CN107449771B (zh) * | 2017-06-28 | 2021-01-01 | 普菲特益斯生物科技(北京)有限公司 | 酶促化学发光底物 |
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CN112794838B (zh) * | 2019-11-13 | 2022-12-23 | 广东菲鹏生物有限公司 | 发光增强剂、酶促化学发光底物及制备方法与试剂盒 |
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CN112067601B (zh) * | 2020-08-05 | 2022-04-22 | 武汉生之源生物科技股份有限公司 | 一种碱性磷酸酶促化学发光底物增强剂及其应用 |
CN114685418B (zh) * | 2020-12-31 | 2023-06-27 | 菲鹏生物股份有限公司 | 用于增强amppd底物的化学发光强度的化合物及其制备方法和增强剂 |
CN114685417B (zh) * | 2020-12-31 | 2023-06-27 | 菲鹏生物股份有限公司 | 用于提升碱性磷酸酶底物发光强度的有机物及其制备方法和增强发光剂 |
CN113295678B (zh) * | 2021-05-14 | 2022-11-08 | 济南迪曼生物科技有限公司 | 一种电化学发光清洗液 |
CN114624205A (zh) * | 2022-02-28 | 2022-06-14 | 海南绿峰资源开发有限公司 | 一种碱性磷酸酶的检测方法 |
CN116429756A (zh) * | 2023-04-21 | 2023-07-14 | 深圳瑞莱柏生物科技有限公司 | 一种碱性磷酸酶的酶促化学发光底物液 |
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WO2016206654A2 (zh) | 2016-12-29 |
CN104990912B (zh) | 2018-04-20 |
CN104990912A (zh) | 2015-10-21 |
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