WO2016185518A1 - 原子間力顕微鏡の情報取得方法 - Google Patents
原子間力顕微鏡の情報取得方法 Download PDFInfo
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- WO2016185518A1 WO2016185518A1 PCT/JP2015/064050 JP2015064050W WO2016185518A1 WO 2016185518 A1 WO2016185518 A1 WO 2016185518A1 JP 2015064050 W JP2015064050 W JP 2015064050W WO 2016185518 A1 WO2016185518 A1 WO 2016185518A1
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- sample
- information
- interaction
- information acquisition
- atomic force
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- 238000000034 method Methods 0.000 title claims abstract description 85
- 239000000523 sample Substances 0.000 claims abstract description 327
- 230000003993 interaction Effects 0.000 claims abstract description 97
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000003786 synthesis reaction Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 29
- 238000013507 mapping Methods 0.000 description 10
- 230000004048 modification Effects 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 230000008846 dynamic interplay Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 210000003632 microfilament Anatomy 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000004651 near-field scanning optical microscopy Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005641 tunneling Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q10/00—Scanning or positioning arrangements, i.e. arrangements for actively controlling the movement or position of the probe
- G01Q10/02—Coarse scanning or positioning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q10/00—Scanning or positioning arrangements, i.e. arrangements for actively controlling the movement or position of the probe
- G01Q10/04—Fine scanning or positioning
- G01Q10/06—Circuits or algorithms therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
- G01Q30/04—Display or data processing devices
Definitions
- the present invention relates to a scanning probe microscope, and more particularly to an atomic force microscope used for observation of a biological sample.
- a scanning probe microscope is a scanning microscope that mechanically scans a mechanical probe to obtain information on a sample surface, and includes a scanning tunneling microscope (STM), an atomic force microscope (AFM), and a scanning type. It is a general term for a magnetic force microscope (MFM), a scanning capacitance microscope (SCaM), a scanning near-field light microscope (SNOM), and the like.
- the scanning probe microscope can perform raster scanning of the mechanical probe and the sample in the XY directions relatively, obtain desired surface information of the sample via the mechanical probe, and display the mapping on the display.
- the atomic force microscope hereinafter referred to as AFM
- AFM atomic force microscope
- a scanner that scans relative to the sample is provided. Then, a mechanical interaction is generated between the mechanical probe and the sample, and information on the sample is obtained based on the deformation of the cantilever generated by the mechanical interaction.
- the interrelationship between the cell surface and the inside of the cell is also important information.
- the conventional AFM for in-vivo moving image observation can acquire the cell surface and the cell interior independently, no attempt has been made to acquire such information in association with each other.
- a probe provided at a free end of a cantilever is brought into contact with a sample and a mechanical interaction is generated between them, and the cantilever and the sample are relatively raster-scanned along the XY plane.
- the present invention is directed to an information acquisition method for an atomic force microscope that acquires sample information.
- the method includes a first interaction generating step for generating a first interaction having a first size between the probe and the sample, and the first interaction between the probe and the sample.
- a first information acquisition step for acquiring first information of the sample when an interaction occurs; and a second interaction having a second size is generated between the probe and the sample.
- the first magnitude of the first interaction and the second magnitude of the second interaction are different from each other.
- the first interaction generation step, the first information acquisition step, the second interaction generation step, and the second information acquisition step are performed in the same scanning region.
- FIG. 1 shows a configuration of an atomic force microscope that can be commonly used in the embodiment.
- FIG. 2 shows the raster scan movement of the cantilever relative to the sample.
- FIG. 3 shows an X scan signal and a Y scan signal for performing the raster scan shown in FIG.
- FIG. 4 is a flowchart of the information acquisition method of the atomic force microscope according to the first embodiment.
- FIG. 5 shows the X scan signal, the Y scan signal, the first information of the sample displayed on the sample information display, and the second information of the sample in the information acquisition method of the first embodiment.
- FIG. 6 shows a cell as a sample.
- FIG. 7 shows an example in which the region F of the cell in FIG. 6 is observed with a reduced interaction.
- FIG. 8 shows an example in which the region F of the cell in FIG. 6 is observed with an increased interaction.
- FIG. 9 shows an example of an observation image displayed on the sample information display by the information acquisition method of the first embodiment.
- FIG. 10A shows the forward path of one scan line of the raster scan of the cantilever with respect to the sample.
- FIG. 10B highlights the return path of one scan line of the cantilever raster scan relative to the sample.
- FIG. 11A shows an X scan signal and a Y scan signal for performing raster scan, and shows an X scan signal portion corresponding to the forward path highlighted in FIG. 10A.
- FIG. 11B shows the X scanning signal and the Y scanning signal for performing raster scanning, and shows the portion of the X scanning signal corresponding to the return path highlighted in FIG.
- FIG. 12 is a flowchart of an atomic force microscope information acquisition method according to the second embodiment.
- FIG. 13 shows the X scan signal, the Y scan signal, the first information of the sample displayed on the sample information display, and the second information of the sample in the information acquisition method of the second embodiment.
- FIG. 14 is a flowchart of an information acquisition method for an atomic force microscope according to a modification of the second embodiment.
- FIG. 15 shows the X scan signal, the Y scan signal, the first information of the sample displayed on the sample information display, and the second information of the sample in the information acquisition method of the modification of the second embodiment.
- FIG. 16 is a flowchart of an information acquisition method for an atomic force microscope according to the third embodiment.
- FIG. 17 shows the X scan signal, the Y scan signal, the first information of the sample displayed on the sample information display, and the second information of the sample in the information acquisition method of the third embodiment.
- FIG. 18 is a flowchart of an information acquisition method for an atomic force microscope according to the fourth embodiment.
- FIG. 19 shows an example of an observation image displayed on the sample information display by the information acquisition method of the fourth embodiment.
- FIG. 20 is a flowchart of an atomic force microscope information acquisition method according to the fifth embodiment.
- FIG. 21 is a flowchart of an information acquisition method for an atomic force microscope according to the sixth embodiment.
- FIG. 1 shows the configuration of an atomic force microscope.
- the atomic force microscope has a cantilever 102 having a probe 101 at the free end.
- the cantilever 102 is arranged so that the probe 101 faces the sample 103.
- This cantilever 102 is held by a holder 104.
- a piezoelectric element 105 is provided on the holder 104.
- the piezoelectric element 105 operates as a vibrator that vibrates the cantilever 102 via the holder 104.
- the piezoelectric element 105 vibrates the cantilever 102 based on the vibration signal output from the controller 110.
- This excitation signal includes an AC component for causing the cantilever 102 to vibrate near its mechanical resonance frequency.
- An optical lever sensor 106 for optically detecting the displacement of the cantilever 102 is disposed on the cantilever 102.
- the optical lever sensor 106 outputs a vibration state signal of the cantilever 102. This vibration state signal is supplied to the controller 110.
- the sample 103 is held on the Z scanner 107 via a sample table (not shown), and the Z scanner 107 is arranged on the XY scanner 108.
- the XY scanner 108 includes an X scanner 108a and a Y scanner 108b.
- the sample 103 is in a liquid cell (not shown).
- the sample 103 is, for example, a living cell in a liquid.
- the Z scanner 107 scans the sample 103 along the Z direction with respect to the cantilever 102.
- the Z scanner 107 is controlled by the controller 110 and expands and contracts along the Z direction based on the Z scanning signal output from the controller 110, thereby causing the sample 103 to scan the cantilever 102 along the Z direction.
- This Z scanning signal is a signal for controlling the Z scanner 107 so as to keep, for example, the amplitude value of the vibration state signal of the cantilever 102 constant, and the Z scanning signal follows the Z direction of the cantilever 102 and the sample 103.
- the relative distance is controlled. That is, the controller 110 can control the magnitude of the dynamic interaction between the probe 101 and the sample 103.
- the XY scanner 108 performs raster scanning of the sample 103 along the XY plane with respect to the cantilever 102.
- the X scanner 108a and the Y scanner 108b constituting the XY scanner 108 are controlled by the controller 110 and displaced along the X direction and the Y direction based on the X scanning signal and the Y scanning signal output from the controller 110, respectively. Thereby, the sample 103 is raster-scanned along the XY plane with respect to the cantilever 102.
- FIG. 2 shows the relative raster scanning movement of the cantilever 102 with respect to the sample 103.
- This raster scanning movement is a conventional one.
- the scanning line direction (the direction in which the scanning speed is high) of raster scanning is the X direction.
- FIG. 3 shows an X scanning signal and a Y scanning signal for performing the raster scanning shown in FIG.
- the controller 110 generates and acquires image data for mapping sample information based on the X scan signal, the Y scan signal, and the Z scan signal, and supplies the image data to the sample information display 111.
- the sample information display 111 is a monitor, for example, and displays image data acquired by the controller 110, that is, sample information.
- An input unit 112 is connected to the controller 110.
- the input unit 112 installs, for example, a program that causes the controller 110 to execute an information acquisition method of each embodiment described later in the controller 110 that controls the atomic force microscope, specifies an observation region, starts observation, and observes. This is for issuing a command such as termination to the controller 110.
- FIG. 4 is a flowchart of the information acquisition method of the atomic force microscope according to the first embodiment.
- FIG. 5 shows the X scan signal, the Y scan signal, the first information of the sample 103 displayed on the sample information display 111, and the second information of the sample 103 in the information acquisition method of the first embodiment. .
- the information acquisition method of the present embodiment is a method of alternately acquiring the first information of the sample 103 and the second information of the sample 103 for each scan line of one raster scan.
- step S101 observation (acquisition of sample information) is started.
- An excitation signal is output from the controller 110 and supplied to the piezoelectric element 105 that operates as a vibrator.
- the piezoelectric element 105 vibrates the cantilever 102 in the vicinity of the mechanical resonance frequency based on the excitation signal.
- the optical lever sensor 106 disposed on the upper part of the cantilever 102 detects the vibration state of the cantilever 102 and supplies a vibration state signal to the controller 110.
- the controller 110 generates a Z scanning signal based on the vibration state signal, and controls the relative distance along the Z direction between the sample 103 and the cantilever 102 by expanding and contracting the Z scanner 107.
- step S102 the controller 110 outputs an X scanning signal and a Y scanning signal.
- the XY scanner 108 receives the X scanning signal and the Y scanning signal, and starts raster scanning along the XY plane of the sample 103 with respect to the cantilever 102.
- step S103 the controller 110 determines whether the current raster scan scanning line (scanning line based on the X scanning signal) is an odd number or an even number.
- the current raster scan scanning line scanning line based on the X scanning signal
- the controller 110 adjusts the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S104.
- a dynamic interaction between the probe 101 and the sample 103 is adjusted, and a first interaction having a first magnitude is generated between the probe 101 and the sample 103.
- the controller 110 increases the relative distance along the Z direction between the cantilever 102 and the sample 103 to reduce the mechanical interaction between the probe 101 and the sample 103, and has a first size having a first size. Is caused between the probe 101 and the sample 103.
- step S105 the controller 110, based on the X scan signal, the Y scan signal, and the Z scan signal, image data for one scan line for mapping the first information of the sample 103, that is, the sample for one scan line.
- First information 103 is generated and acquired.
- the first information of the sample 103 is information on the sample when a small interaction occurs between the probe 101 and the sample 103, for example, information on the surface of the sample.
- step S106 the sample information display unit 111 scans the image data for one scanning line acquired by the controller 110, that is, the first information of the sample 103 for one scanning line in the area A shown in FIG. Display lines.
- the scanning line is odd-numbered, that is, when the X scanning signal is in the section A shown in FIG. 5, the sample information display 111 sends the first information of the sample 103 to the area A shown in FIG. indicate.
- step S103 if the scanning line is an even number, for example, the second, the controller 110 reduces the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S107.
- the dynamic interaction between the probe 101 and the sample 103 is increased, and a second interaction having a second size is generated between the probe 101 and the sample 103.
- the second magnitude of the second interaction is greater than the first magnitude of the first interaction.
- step S108 the controller 110, based on the X scan signal, the Y scan signal, and the Z scan signal, image data for one scan line for mapping the second information of the sample 103, that is, the sample for one scan line.
- the second information 103 is generated and acquired.
- the second information of the sample 103 is sample information when a large interaction occurs between the probe 101 and the sample 103, for example, information inside the sample.
- step S109 the sample information display unit 111 scans the image data for one scanning line acquired by the controller 110, that is, the second information of the sample 103 for one scanning line in the region B shown in FIG. Display lines.
- the scanning line is even-numbered, that is, when the X scanning signal is in the section B shown in FIG. 5, the sample information display 111 sends the second information of the sample 103 to the region B shown in FIG. indicate.
- step S110 the controller 110 determines whether one raster scan has been completed. If the result of determination in step S110 is that one raster scan has not been completed, the process returns to step S103, and steps S103 to S110 are repeated until one raster scan is completed. As a result, the first information of the sample 103 displayed in the area A of FIG. 5 and the second information of the sample 103 displayed in the area B of FIG. 5 are alternately updated and displayed at equal time intervals.
- step S111 the controller 110 determines whether to end observation, in other words, whether to perform the next raster scan, that is, to perform observation again (to acquire the sample information again).
- step S111 If the result of determination in step S111 is to perform observation again, the process returns to step S102.
- step S111 If the observation is ended as a result of the determination in step S111, the observation is ended in step S112.
- information on two types of samples whose magnitude of the dynamic interaction between the probe 101 and the sample 103 is large is measured for each scan line, in other words, for one scan line. Acquired alternately at equal time intervals corresponding to scanning. This can be regarded as acquiring information of two types of samples almost simultaneously. Therefore, it becomes possible to obtain information on their mutual relationship.
- sample 103 is the cell shown in FIG.
- region F in FIG. 6 is observed by AFM.
- FIG. 7 shows an example in which the cell region F in FIG. 6 is observed with a reduced interaction.
- the interaction is small, the cell membrane surface information of the cell can be obtained. Therefore, depending on the cell type, a smooth cell surface shape with almost no unevenness as shown in FIG. 7 can be obtained.
- This information is displayed in region A of the sample information display 111.
- FIG. 8 shows an example in which the region F of the cell in FIG. 6 is observed with an increased interaction.
- the interaction is large, information on the inside of the cell (organelle) can be obtained.
- FIG. 8 shows, for example, an image of a cytoskeleton (actin filament). This information is displayed in region B of the sample information display 111.
- FIG. 9 shows an example of an observation image displayed on the sample information display by the information acquisition method of the present embodiment.
- the interaction is small in the region A and the region B of the sample information display 111, respectively.
- the state where the display of all the lines of the second information of the sample 103 when the interaction with the first information of the sample 103 is large is shown.
- cell surface information and internal information can be acquired almost simultaneously, and as a result, it is possible to obtain information on their correlation.
- FIG. 10A and FIG. 10B show the raster scanning movement of the cantilever with respect to the sample, and the forward and backward paths of one scanning line of the raster scanning are highlighted.
- 11A and 11B show the X scanning signal and the Y scanning signal for performing the raster scanning, and the X scanning signals corresponding to the forward path and the backward path highlighted in FIGS. 10A and 10B, respectively. The part is highlighted.
- the forward path of scanning in raster scanning is in the + X direction as shown in FIG. 10A, and the X scanning signal corresponding thereto is a portion that rises to the right as shown in FIG. 11A.
- the scanning return path in the raster scanning is in the ⁇ X direction as shown in FIG. 10B, and the X scanning signal corresponding to the backward path is a lower right portion as shown in FIG. 11B.
- FIG. 12 is a flowchart of an atomic force microscope information acquisition method according to the second embodiment.
- FIG. 13 shows the X scan signal, the Y scan signal, the first information of the sample 103 displayed on the sample information display, and the second information of the sample 103 in the information acquisition method of the second embodiment.
- the first information of the sample 103 and the second information of the sample 103 are alternately obtained in units of one round trip in the X direction.
- the first information of the sample 103 and the second information of the sample 103 are obtained alternately by using one half of one reciprocal scan in the X direction, that is, the forward or return path as a unit.
- step S201 observation (sample information acquisition) is started. Details of step S201 are the same as step S101 of the first embodiment, and details thereof are omitted.
- step S202 the controller 110 outputs an X scanning signal and a Y scanning signal.
- the XY scanner 108 receives the X scanning signal and the Y scanning signal, and starts raster scanning along the XY plane of the sample 103 with respect to the cantilever 102.
- step S203 the controller 110 determines whether the current raster scan scanning line (scanning line based on the X scanning signal) is the forward path or the backward path.
- the current raster scan scanning line scanning line based on the X scanning signal
- one scanning line corresponds to one of the forward path and the backward path in the X direction.
- the controller 110 adjusts the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S204, and between the probe 101 and the sample 103.
- the first interaction having the first magnitude is generated between the probe 101 and the sample 103.
- the controller 110 increases the relative distance along the Z direction between the cantilever 102 and the sample 103 to reduce the mechanical interaction between the probe 101 and the sample 103, and has a first size having a first size. Is caused between the probe 101 and the sample 103.
- step S205 the controller 110, based on the X scan signal, the Y scan signal, and the Z scan signal, image data for one forward path for mapping the first information of the sample 103, that is, the sample 103 for one forward path. Generate and obtain first information.
- the first information of the sample 103 is information on the sample when a small interaction occurs between the probe 101 and the sample 103, for example, information on the surface of the sample.
- step S206 the sample information display 111 displays the image data for one outbound path acquired by the controller 110, that is, the first information of the sample 103 for one outbound path in the area A shown in FIG. To do.
- the scanning line is the forward path, that is, when the X scanning signal is in the section A shown in FIG. 13
- the sample information display 111 displays the first information of the sample 103 in the area A shown in FIG. To do.
- step S203 determines whether the current scanning line is the return path. If the result of the determination in step S203 is that the current scanning line is the return path, the controller 110 reduces the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S207 to reduce the distance between the probe 101 and the sample 103. And a second interaction having a second size is generated between the probe 101 and the sample 103.
- the second magnitude of the second interaction is greater than the first magnitude of the first interaction.
- step S208 the controller 110, based on the X scanning signal, the Y scanning signal, and the Z scanning signal, image data for one return path for mapping the second information of the sample 103, that is, the sample 103 for one return path. Generate and obtain second information.
- the second information of the sample 103 is sample information when a large interaction occurs between the probe 101 and the sample 103, for example, information inside the sample.
- step S209 the sample information display 111 displays the image data for one return path acquired by the controller 110, that is, the second information of the sample 103 for one return path in the area B shown in FIG. To do.
- the scanning line is the return path, that is, when the X scanning signal is in the section B shown in FIG. 13
- the sample information display 111 displays the second information of the sample 103 in the region B shown in FIG. To do.
- step S210 the controller 110 determines whether or not one raster scan has been completed. If the result of determination in step S210 is that one raster scan has not been completed, the process returns to step S203, and steps S203 to S210 are repeated until one raster scan is completed.
- step S211 the controller 110 determines whether to end observation, in other words, whether to perform observation again.
- step S211 If the result of determination in step S211 is to perform observation again, the process returns to step S202.
- step S212 If the observation is terminated as a result of the determination in step S211, the observation is terminated in step S212.
- the first information of the sample 103 when the small interaction is generated and the second information of the sample 103 when the large interaction are generated are respectively sent to the forward path of the raster scanning.
- On the return path in other words, it is acquired alternately at equal time intervals. This can be regarded as acquiring information of two types of samples almost simultaneously. Therefore, it becomes possible to obtain information on their mutual relationship.
- cell surface information and internal information can be acquired almost simultaneously, and as a result, it is possible to obtain information on their correlation.
- FIG. 14 is a flowchart of an information acquisition method for an atomic force microscope according to a modification of the second embodiment.
- FIG. 15 shows the X scan signal, the Y scan signal, the first information of the sample 103 displayed on the sample information display, and the second information of the sample 103 in the information acquisition method of the modification of the second embodiment. ing.
- the sample information is acquired alternately only in the raster scan forward path. That is, the sample information is alternately obtained in the odd-numbered and even-numbered outbound paths of raster scanning.
- step S203 in the flowchart shown in FIG. 12 is replaced with steps S203A and S203B as shown in FIG.
- step S201 observation (sample information acquisition) is started.
- step S202 the controller 110 outputs an X scanning signal and a Y scanning signal.
- the XY scanner 108 receives the X scanning signal and the Y scanning signal, and starts raster scanning along the XY plane of the sample 103 with respect to the cantilever 102.
- step S203A the controller 110 determines whether the current raster scanning scanning line (scanning line based on the X scanning signal) is the forward path or the backward path.
- the current raster scanning scanning line scanning line based on the X scanning signal
- one scanning line corresponds to one of the forward path and the backward path in the X direction.
- step S203A If the result of determination in step S203A is that the current scan line is a return path, the process returns to step S203A again.
- step S203B the controller 110 determines whether the current outbound path is an odd number or an even number.
- step S203B if the current scan line is an odd number, the controller 110 increases the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S204 and increases the relative distance between the probe 101 and the sample 103.
- a first interaction having a first size is generated between the probe 101 and the sample 103.
- step S205 the controller 110, based on the X scan signal, the Y scan signal, and the Z scan signal, image data for one forward path for mapping the first information of the sample 103, that is, the sample 103 for one forward path. Generate and obtain first information.
- the first information of the sample 103 is information on the sample when a small interaction occurs between the probe 101 and the sample 103, for example, information on the surface of the sample.
- step S206 the sample information display 111 displays the image data for one forward path acquired by the controller 110, that is, the first information of the sample 103 for one forward path in the area A shown in FIG. To do.
- the scan line is an odd-numbered outbound path, that is, when the X scan signal is in the section A shown in FIG. 15, the sample information display 111 displays the first information of the sample 103 in the area shown in FIG. A is displayed.
- step S203B if the current scanning line is an even-numbered forward path, the controller 110 reduces the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S207, and the probe 101 and the sample.
- the mechanical interaction between the probes 103 and the sample 103 is generated by increasing the dynamic interaction between the probes 103 and the second interaction having the second magnitude.
- the second magnitude of the second interaction is greater than the first magnitude of the first interaction.
- step S208 the controller 110, based on the X scanning signal, the Y scanning signal, and the Z scanning signal, image data for one path for mapping the second information of the sample 103, that is, the sample 103 for one path. Generate and obtain second information.
- the second information of the sample 103 is sample information when a large interaction occurs between the probe 101 and the sample 103, for example, information inside the sample.
- step S209 the sample information display unit 111 displays the image data for one forward path acquired by the controller 110, that is, the second information of the sample 103 for one forward path in the area B shown in FIG. To do.
- the scanning line is an even-numbered forward path, that is, when the X scanning signal is in the section B shown in FIG. 15, the sample information display 111 displays the second information of the sample 103 in the area shown in FIG. B is displayed.
- step S210 the controller 110 determines whether or not one raster scan has been completed. If the result of determination in step S210 is that one raster scan has not been completed, the process returns to step S203A, and steps S203A, S203B, and steps S204 to S210 are repeated until one raster scan is completed.
- step S211 the controller 110 determines whether to end observation, in other words, whether to perform observation again.
- step S211 If the result of determination in step S211 is to perform observation again, the process returns to step S202.
- step S212 If the observation is terminated as a result of the determination in step S211, the observation is terminated in step S212.
- the first information of the sample 103 when the small interaction is generated and the second information of the sample 103 when the large interaction is generated are used as the forward path of the raster scanning. Get alternately only in. This can be regarded as acquiring information of two types of samples almost simultaneously. Therefore, it becomes possible to obtain information on their mutual relationship.
- FIG. 16 is a flowchart of an information acquisition method for an atomic force microscope according to the third embodiment.
- FIG. 17 shows the X scan signal, the Y scan signal, the first information of the sample 103 displayed on the sample information display, and the second information of the sample 103 in the information acquisition method of the third embodiment.
- the information acquisition method of the present embodiment performs raster scanning alternately at least once with respect to the observation region, that is, first raster scanning and second raster scanning are alternately performed, and the first raster scanning is performed.
- the first information of the sample 103 is acquired during the second raster scan
- the second information of the sample 103 is acquired during the second raster scan that is the second raster scan.
- step S301 observation (acquisition of sample information) is started. Details of step S301 are the same as step S101 of the first embodiment, and details thereof are omitted.
- step S302 the controller 110 outputs an X scanning signal and a Y scanning signal.
- the XY scanner 108 receives the X scanning signal and the Y scanning signal, and starts raster scanning along the XY plane of the sample 103 with respect to the cantilever 102.
- step S303 the controller 110 determines whether or not the current raster scan is the first raster scan.
- step S303 If it is determined in step S303 that the current raster scan is the first raster scan, the controller 110 increases the relative distance along the Z direction between the cantilever 102 and the sample 103 in step S304. The mechanical interaction between the sample 103 is reduced, and a first interaction having a first size is generated between the probe 101 and the sample 103.
- step S305 the controller 110 generates and acquires image data for mapping the first information of the sample 103 based on the X scanning signal, the Y scanning signal, and the Z scanning signal.
- the first information of the sample 103 is information on the sample when a small interaction occurs between the probe 101 and the sample 103, for example, information on the surface of the sample.
- the first raster scanning includes step S304 and step S305.
- step S306 the sample information display 111 displays the image data for one raster scan acquired by the controller 110, that is, the first information of the sample 103 in the area A shown in FIG.
- the sample information display 111 displays the first information of the sample 103 in the area A shown in FIG.
- step S307 the controller 110 reduces the relative distance along the Z direction between the cantilever 102 and the sample 103 and the probe 101 and the sample.
- the mechanical interaction between the probes 103 and the sample 103 is generated by increasing the dynamic interaction between the probes 103 and the second interaction having the second magnitude.
- the second magnitude of the second interaction is greater than the first magnitude of the first interaction.
- step S308 the controller 110 generates and acquires image data for mapping the second information of the sample 103 based on the X scanning signal, the Y scanning signal, and the Z scanning signal.
- the second information of the sample 103 is sample information when a large interaction occurs between the probe 101 and the sample 103, for example, information inside the sample.
- the second raster scan includes step S307 and step S308.
- step S309 the sample information display unit 111 displays the image data for one raster scan acquired by the controller 110, that is, the second information of the sample 103 in the region B shown in FIG.
- the sample information display 111 displays the second information of the sample 103 in the area B shown in FIG.
- step S310 the controller 110 determines whether the second raster scan has been completed.
- step S310 If it is determined in step S310 that the second raster scan has not been completed, the process returns to step S302, and steps S302 to S310 are repeated until the second raster scan is completed.
- step S310 when the second raster scan is completed, the controller 110 determines in step S311 whether to end observation, in other words, whether to perform observation again.
- step S311 If the result of determination in step S311 is to perform observation again, the process returns to step S302.
- step S311 If the observation is terminated as a result of the determination in step S311, the observation is terminated in step S312.
- the first raster scan and the second raster scan are alternately performed at least once.
- the first information of the sample 103 when the small interaction is generated and the second information of the sample 103 when the large interaction are generated are alternately changed in time corresponding to one raster scan. It is possible to obtain at least once at equal intervals. This can be regarded as acquiring information of two types of samples almost simultaneously when the scanning speed is sufficiently high. Therefore, it becomes possible to obtain information on their mutual relationship.
- cell surface information and internal information can be acquired almost simultaneously, and as a result, information on their correlation can be obtained.
- FIG. 18 is a flowchart of the information acquisition method of the fourth embodiment.
- FIG. 19 shows an example of an observation image displayed on the sample information display by the information acquisition method of the fourth embodiment.
- the information acquisition method of this embodiment is similar to the information acquisition method of the first embodiment.
- the information acquisition method of the present embodiment includes the steps of the sample 103 between step S110 and step S111 in addition to the steps of the information acquisition method of the first embodiment.
- Step S401 that calculates and displays one piece of information and second piece information of the sample 103 is included.
- the controller 110 calculates the first information of the sample 103 when the interaction displayed in each of the region A and the region B is small and the second information of the sample 103 when the interaction is large.
- the third information 103 is acquired.
- the sample information display 111 displays the third information of the sample 103 acquired by the controller 110 in the area C shown in FIG.
- the calculation for acquiring the third information of the sample 103 may be, for example, synthesis or addition, but is not limited to this, and it is desirable that the calculation is properly used according to information to be obtained such as subtraction or division.
- the first information and the second information of the sample 103 are combined.
- the third information of the sample 103 is displayed in the area C.
- cell surface information and internal information can be acquired almost simultaneously, and further, the positional relationship between the surface and the inside can be clarified by synthesizing them. This makes it possible to obtain more detailed information on the interrelation between the surface and the interior.
- FIG. 20 is a flowchart of the information acquisition method of the fifth embodiment.
- the information acquisition method of this embodiment is similar to the information acquisition method of the second embodiment.
- the information acquisition method of the present embodiment includes the steps of the sample 103 between step S210 and step S211 in addition to the steps of the information acquisition method of the second embodiment.
- Step S501 for calculating and displaying one information and the second information of the sample 103 is included.
- the controller 110 calculates the first information of the sample 103 when the interaction displayed in each of the regions A and B is small and the second information of the sample 103 when the interaction is large, thereby performing the sample.
- the third information 103 is acquired.
- the sample information display 111 displays the third information of the sample 103 acquired by the controller 110.
- the calculation for acquiring the third information of the sample 103 may be, for example, synthesis or addition, but is not limited to this, and it is desirable that the calculation is properly used according to information to be obtained such as subtraction or division.
- cell surface information and internal information can be acquired almost simultaneously, and further, the positional relationship between the surface and the inside can be clarified by synthesizing them. This makes it possible to obtain more detailed information on the interrelation between the surface and the interior.
- FIG. 21 is a flowchart of the information acquisition method of the sixth embodiment.
- the information acquisition method of this embodiment is similar to the information acquisition method of the third embodiment.
- the information acquisition method of the present embodiment includes the steps of the sample 103 between step S310 and step S311. Step S601 for calculating and displaying one piece of information and the second piece of information of the sample 103 is included.
- step S601 the controller 110 calculates the first information of the sample 103 having a small interaction and the second information of the sample 103 having a large interaction displayed in the areas A and B, respectively, and calculates a third sample. Get information.
- the sample information display 111 displays the third information of the sample 103 acquired by the controller 110.
- the calculation for acquiring the third information of the sample 103 may be, for example, synthesis or addition, but is not limited to this, and it is desirable that the calculation is properly used according to information to be obtained such as subtraction or division.
- cell surface information and internal information can be acquired almost simultaneously, and further, the positional relationship between the surface and the inside can be clarified by synthesizing them. This makes it possible to obtain more detailed information on the interrelation between the surface and the interior.
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Abstract
Description
原子間力顕微鏡の情報取得方法の実施形態の説明に先立ち、実施形態において共通に使用され得る原子間力顕微鏡の構成について図1~図3を用いて説明する。図1は、原子間力顕微鏡の構成を示している。
次に、第一実施形態による原子間力顕微鏡の情報取得方法について図4と図5を用いて説明する。図4は、第一実施形態による原子間力顕微鏡の情報取得方法のフローチャートである。また図5は、第一実施形態の情報取得方法におけるX走査信号とY走査信号と試料情報表示器111に表示される試料103の第一の情報と試料103の第二の情報を示している。
第二実施形態による原子間力顕微鏡の情報取得方法の説明に先立ち、ラスター走査の走査ラインの往路と復路について図10Aと図10Bと図11Aと図11Bを用いて説明する。
次に、第二実施形態の変形例による原子間力顕微鏡の情報取得方法について図14と図15を用いて説明する。図14は、第二実施形態の変形例による原子間力顕微鏡の情報取得方法のフローチャートである。また図15は、第二実施形態の変形例の情報取得方法におけるX走査信号とY走査信号と試料情報表示器に表示される試料103の第一の情報と試料103の第二の情報を示している。図14において、図12と同一の参照符号で示されたステップは同様のステップを示しており、続く説明ではその詳細は省略する。
第三実施形態による原子間力顕微鏡の情報取得方法について図16と図17を用いて説明する。図16は、第三実施形態による原子間力顕微鏡の情報取得方法のフローチャートである。また図17は、第三実施形態の情報取得方法におけるX走査信号とY走査信号と試料情報表示器に表示される試料103の第一の情報と試料103の第二の情報を示している。
本実施形態の情報取得方法について図18と図19を用いて説明する。図18は、第四実施形態の情報取得方法のフローチャートである。また図19は、第四実施形態の情報取得方法によって試料情報表示器に表示される観察画像の例を示している。
第五実施形態の情報取得方法について図20を用いて説明する。図20は、第五実施形態の情報取得方法のフローチャートである。本実施形態の情報取得方法は、第二実施形態の情報取得方法に似ている。図12と図20を比較して分かるように、本実施形態の情報取得方法は、第二実施形態の情報取得方法の諸ステップに加えて、ステップS210とステップS211の間に、試料103の第一の情報と試料103の第二の情報を演算して表示するステップS501を有している。ステップS501において、コントローラ110は、領域Aと領域Bにそれぞれ表示された相互作用が小さいときの試料103の第一の情報と相互作用が大きいときの試料103の第二の情報を演算して試料103の第三の情報を取得する。また、試料情報表示器111は、コントローラ110で取得された試料103の第三の情報を表示する。試料103の第三の情報を取得する演算は、例えば合成すなわち加算であってよいが、これに限らず、減算や除算など、得たい情報によって使い分けられることが望ましい。
第六実施形態の情報取得方法について図21を用いて説明する。図21は、第六実施形態の情報取得方法のフローチャートである。本実施形態の情報取得方法は、第三実施形態の情報取得方法に似ている。図16と図21を比較して分かるように、本実施形態の情報取得方法は、第三実施形態の情報取得方法の諸ステップに加えて、ステップS310とステップS311の間に、試料103の第一の情報と試料103の第二の情報を演算して表示するステップS601を有している。ステップS601において、コントローラ110は、Aの領域とBの領域にそれぞれ表示した相互作用が小さい試料103の第一の情報と相互作用が大きい試料103の第二の情報を演算して第三の試料情報を取得する。また、試料情報表示器111は、コントローラ110で取得された試料103の第三の情報を表示する。試料103の第三の情報を取得する演算は、例えば合成すなわち加算であってよいが、これに限らず、減算や除算など、得たい情報によって使い分けられることが望ましい。
Claims (11)
- カンチレバーの自由端に設けられた探針を試料と接触させ、それらの間に力学的な相互作用を生じさせながら、XY平面に沿って前記カンチレバーと前記試料を相対的にラスター走査させて前記試料の情報を取得する原子間力顕微鏡の情報取得方法であって、
前記探針と前記試料の間に第一の大きさを有する第一の相互作用を生じさせる第一の相互作用生成工程と、
前記探針と前記試料の間に前記第一の相互作用が生じているときの前記試料の第一の情報を取得する第一の情報取得工程と、
前記探針と前記試料の間に、前記第一の大きさと異なる第二の大きさを有する第二の相互作用を生じさせる第二の相互作用生成工程と、
前記探針と前記試料の間に前記第二の相互作用が生じているときの前記試料の第二の情報を取得する第二の情報取得工程と、を有し、
前記第一の相互作用生成工程および前記第一の情報取得工程と、前記第二の相互作用生成工程および前記第二の情報取得工程は同一走査領域において行われる、原子間力顕微鏡の情報取得方法。 - 前記第一の相互作用生成工程および前記第一の情報取得工程と、前記第二の相互作用生成工程および前記第二の情報取得工程は、前記ラスター走査の走査ライン毎に交互に繰り返される、請求項1記載の原子間力顕微鏡の情報取得方法。
- 前記ラスター走査の走査ラインは、走査の往路と復路の少なくとも一方である、請求項2記載の原子間力顕微鏡の情報取得方法。
- 前記第一の相互作用生成工程と前記第一の情報取得工程とを含む第一のラスター走査工程と、
前記第二の相互作用生成工程と前記第二の情報取得工程とを含む第二のラスター走査工程と、を備え、
前記第一のラスター走査工程と前記第二のラスター走査工程は、交互に少なくとも1回ずつ行われる、請求項1記載の原子間力顕微鏡の情報取得方法。 - 前記試料の第一の情報と前記試料の第二の情報を、時間的等間隔で交互にそれぞれ更新表示する第一の情報表示工程と第二の情報表示工程を有している、請求項1~4のいずれかひとつに記載の原子間力顕微鏡の情報取得方法。
- 前記第二の相互作用の前記第二の大きさは前記第一の相互作用の前記第一の大きさよりも大きく、
前記試料は、細胞であり、
前記試料の第一の情報は、前記細胞の表面の情報であり、
前記試料の第二の情報は、前記細胞の内部の情報である、請求項1~5のいずれかひとつに記載の原子間力顕微鏡の情報取得方法。 - 前記試料の第一の情報と前記試料の第二の情報を演算することにより前記試料の第三の情報を生成する情報生成工程をさらに有している、請求項1~6のいずれかひとつに記載の原子間力顕微鏡の情報取得方法。
- 前記演算は合成演算である、請求項7に記載の原子間力顕微鏡の情報取得方法。
- 前記試料の第三の情報を表示する第三の情報表示工程をさらに有している、請求項7または8に記載の原子間力顕微鏡の情報取得方法。
- 請求項1~9のいずれかひとつに記載の原子間力顕微鏡の情報取得方法を原子間力顕微鏡に実行させるプログラム。
- 請求項10に記載のプログラムがコントローラにインストールされた原子間力顕微鏡。
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DE112015006610T5 (de) * | 2015-07-07 | 2018-03-08 | Olympus Corporation | Rasterkraftmikroskop und Steuerverfahren dafür |
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