WO2016066062A1 - Appareil d'ionisation par électronébuliseur et spectromètre de masse - Google Patents

Appareil d'ionisation par électronébuliseur et spectromètre de masse Download PDF

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Publication number
WO2016066062A1
WO2016066062A1 PCT/CN2015/092690 CN2015092690W WO2016066062A1 WO 2016066062 A1 WO2016066062 A1 WO 2016066062A1 CN 2015092690 W CN2015092690 W CN 2015092690W WO 2016066062 A1 WO2016066062 A1 WO 2016066062A1
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Prior art keywords
liquid
tip
electrospray ionization
potential difference
electrospray
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PCT/CN2015/092690
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English (en)
Chinese (zh)
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黄云清
蒋公羽
孙文剑
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岛津分析技术研发（上海）有限公司
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Publication of WO2016066062A1 publication Critical patent/WO2016066062A1/fr

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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes

Definitions

  • the invention relates to the technical field of mass spectrometry, in particular to an electrospray ionization device and a mass spectrometer.
  • Chromatography-mass spectrometry combines the high separation efficiency of chromatography with the high selectivity and sensitivity of mass spectrometry. It is still the most effective method for complex sample analysis. However, because the chromatographic separation process cannot be completed in a short period of time, the chromatographic-mass spectrometry method is not a high-throughput analytical method, and it is not sufficient to meet certain analytical tasks that require results to be available or large sample sizes.
  • Instrumentation for flow injection mass spectrometry typically includes a solvent delivery device, an injection valve, and a mass spectrometer ionization source that are connected together by a solvent delivery line.
  • Instrumentation for flow injection mass spectrometry typically includes a solvent delivery device, an injection valve, and a mass spectrometer ionization source that are connected together by a solvent delivery line.
  • the injection valve When the injection valve is in the load position, the sample solution is injected into the sample loop through the syringe. At this point, the solvent flows into the mass spectrometry ion source through a separate channel on the valve; when the sample is injected, the injection valve is switched. To the injection state, the flow of solvent on the valve is routed to the sample loop by the separate channel described above, and the injected sample is brought into the mass spectrometry ionization source.
  • the use of the auto-injection robot greatly improves the analysis speed of the flow injection-mass spectrometry method, making the technology a metabolomics, clinical test and related pharmacodynamics, toxicology and mechanism research requiring large sample and large data volume acquisition and analysis. Have certain advantages.
  • this method also has the disadvantages of: 1) requiring proper sample preparation to remove a large amount of background matrix that inhibits ionization in the sample solution, which limits the increase in analytical throughput; 2) syringes, tubing, and ionization sources The needle will have analyte residue, even a small amount of residue will make the quantitative analysis of the quantitative analysis have obvious deviation; 3) After the contamination occurs, the replacement of the needle, the pipeline and the ionization source needle is very cumbersome.
  • U.S. Patent No. 6,858,437 B2 discloses a direct flow injection analysis atomization electrospray technique which is unique in that a syringe is used in place of a conventional electrospray ionization source.
  • a syringe draws the sample solution, it is directly inserted into the probe that closely fits the syringe, and a DC voltage is applied to the syringe, and if necessary, an auxiliary atomizing gas is passed through the gap between the needle and the probe.
  • the syringe can be a multi-use needle or a disposable tip.
  • Simple sample preparation can be achieved by filling the sample with a chromatographic packing to reduce the suppression of ionization by the background matrix.
  • the use of atomizing gas can reduce the voltage amplitude required for electrospray, improve spray stability and desolvation efficiency.
  • Contaminants left on the probe may interfere with the next analysis; 2) if plastic is used
  • the disposable tip of the material the direct electricity needs to be contacted with the sample solution in the tip through a metal part, which not only increases the complexity of the device, but also the possibility of contaminants remaining on the metal part.
  • the traditional pipetting method uses a pipette and uses a visual method to perform a volumetric pipetting method.
  • the pipette used can be reused after washing. Although the pipetting method has high accuracy, the efficiency is too low.
  • Pipetting methods using pipetting guns combined with low-cost disposable plastic tips have become mainstream. The method has the advantages of high pipetting speed, low cost and no cross-contamination between multiple pipetting.
  • the plastic tips used are standardized for mass production and the current purchase price is very low. Williams et al. (Rapid Communication in Mass Spectrometry, 2001, Vol. 15, pp. 1890-1891) used a disposable pipette tip as an electrospray needle to achieve mass spectrometric analysis of organic synthesis intermediates.
  • the applied DC voltage is applied to the tip of the tip by a metal needle inserted into the tip to contact the solution. Since the metal needle is directly in contact with the solution, it needs to be cleaned in multiple analyses, making the analysis process still complicated. Since the atomization gas is not used to assist the generation of electrospray, the spray voltage required for the method is high, and the stability and reproducibility of the electrospray are insufficient.
  • the rigidity of the tip of such a fine polypropylene plastic is insufficient, and the degree of bending between the tip and the tip is not Again, this obviously affects the reproducibility of the tip electrospray.
  • it is necessary to use a tip with a more rigid tip for example, a 20 ⁇ L tip having a tip outer diameter of 750 ⁇ m.
  • the dielectric barrier electrospray is required to achieve a spray of the solution, which requires that the peak-to-peak value of the applied square wave AC voltage is higher than that of the DC electrospray, and with the diameter of the tip of the needle. It increases and increases, and when the peak-to-peak value of the AC voltage is too high, it is easy to cause discharge of the electrode and the surrounding environment.
  • Electrospray ionization in order to develop an electrospray ionization mass spectrometry method which can be used for direct rapid analysis, good reproducibility and no cross-contamination, the above method should be improved to achieve on a tip with a larger tip size. Electrospray ionization.
  • the object of the present invention is to develop an electrospray ionization device which can be used for direct and rapid analysis of a mass spectrometer, has good reproducibility and is free of cross-contamination, and can be used without the electrode contacting the solution. To achieve electrospray ionization on a disposable tip with a larger tip size.
  • the present invention provides an electrospray ionization device for ionizing an analyte in a solution
  • the electrospray ionization device comprising: an ionization chamber; a liquid tip for guiding the The solution enters the ionization chamber in the form of droplets from the outlet end of the liquid tip, and at least a portion of the liquid tip maintains a potential difference relative to the surface of the ionization chamber for a predetermined time to allow liquid to enter the ionization chamber Dropping electrification; one or more air guiding tubes for guiding one or more air currents into the ionization chamber, the one or more air currents directed to the outlet end of the liquid suction head, and the air guiding tube and the liquid suction
  • the extending direction of the head is at a predetermined angle; wherein the air guiding tube and the liquid suction head are disposed to be freely separable; the ion sampling port has an open end facing the ionization chamber for
  • the liquid tip is a tapered liquid tip having a tapered tip as the outlet end.
  • the outer diameter of the tapered tip of the liquid tip has a diameter ranging from 10 to 50 ⁇ m; or 50 to 200 ⁇ m; or 200 to 500 ⁇ m; or 500 to 1000 ⁇ m; or 1000 to 2000 ⁇ m.
  • a chromatographic packing is disposed in the liquid tip.
  • the material used for the chromatographic packing comprises: one or more mixtures of porous silica gel particles, paper fiber porous monolithic materials, polymer monolithic columns, and polymer coated particles.
  • the solution passes through the liquid tip under the pressure difference applied between the inlet end and the outlet end of the liquid tip, wherein the pressure at the inlet end of the liquid tip is stronger than the pressure at the outlet end of the liquid tip.
  • the airflow sent by the air guiding tube passes through the outlet end of the liquid suction head to form a pressure difference between the inlet end of the liquid suction head and the outlet end of the liquid suction head, and the solution passes through the liquid suction head by the pressure difference.
  • the inlet end of the liquid suction head is connected to atmospheric pressure, and the pressure at the outlet end of the liquid suction head is a negative pressure.
  • the potential difference is a DC high voltage potential difference, and the DC potential difference is directly applied to the liquid in the liquid tip.
  • the potential difference is an alternating high voltage potential difference, and the alternating current potential difference is directly applied to the liquid in the liquid tip.
  • the potential difference is an alternating high voltage potential difference
  • the alternating current potential difference is applied to the liquid in the liquid tip through the dielectric.
  • the material of the liquid tip comprises: one of a metal, a quartz tube, an organic polymer material, and glass.
  • the DC high voltage potential difference ranges from 1000 to 3000V; or 3000 to 5000V; or 5000 to 8000V; or 8000 to 12000V.
  • the peak-to-peak value of the alternating high voltage potential ranges from 1000 to 3000V; or 3000 to 5000V; or 5000 to 8000V; or 8000 to 12000V.
  • the frequency of the alternating current potential is 2 to 1000 Hz.
  • the end of the air guiding tube pointing to the outlet end of the liquid suction head has a circular shape, a flat shape, or a circular arc shape.
  • the angle between the air guiding tube and the liquid tip is in the range of 20° to 60°; or 60° to 90°; or 90° to 120°.
  • the air flow is a heated air flow.
  • the airflow speed ranges from 10 to 100 m/s; or from 100 to 300 m/s; or from 300 to 600 m/s; or from 600 to 800 m/s.
  • the present invention provides a mass spectrometer comprising: an electrospray ionization device as described above; and the mass analyzer located below the electrospray ionization device.
  • the present invention provides an electrospray ionization apparatus for ionizing a solution to be analyzed, comprising: an ionization chamber; a liquid tip, guiding the solution into the ionization chamber from the outlet end of the tip in the form of droplets.
  • the tip maintains a potential difference relative to the surface of the ionization chamber for a predetermined time to charge the droplets entering the ionization chamber; one or more air tubes directing a portion of the outlet end of the liquid tip or The plurality of air flows enter the ionization chamber, and the direction of the air flow moves at a predetermined angle with the extending direction of the liquid tip; wherein the air pipe and the liquid tip are freely separable; the ion sampling port has an open end facing the ionization cavity, guiding The analyte ions of the solution enter the mass analyzer located in the lower stage of the electrospray ionization device; the ionization device that achieves direct rapid mass analysis, good reproducibility and no cross-contamination can be realized at the tip size without the electrode contacting the solution. Electrospray ionization is achieved on larger disposable tips.
  • FIG. 1 is a schematic view showing the structure of an electrospray ionization apparatus according to an embodiment of the present invention.
  • FIG. 2 is a schematic view showing the structure of an electrospray ionization apparatus according to still another embodiment of the present invention.
  • FIG. 3 is a schematic view showing the structure of an electrospray ionization apparatus in still another embodiment of the present invention.
  • FIG. 4 is a schematic view showing the structure of an electrospray ionization apparatus according to another embodiment of the present invention.
  • Fig. 5 is a view showing the experimental results of the mass spectrometry application of the electrospray ionization apparatus in an application example of the present invention.
  • FIG. 6 is a schematic view showing experimental results of mass spectrometry application of an electrospray ionization apparatus in an application example of the present invention.
  • FIG. 7 is a schematic view showing experimental results of mass spectrometry application of an electrospray ionization apparatus in an application example of the present invention.
  • Figure 8 is a graph showing experimental results of mass spectrometry application of an electrospray ionization apparatus in an applied embodiment of the present invention.
  • Figure 9 is a graph showing experimental results of mass spectrometry application of an electrospray ionization apparatus in an applied embodiment of the present invention.
  • the present invention provides an embodiment of an electrospray ionization apparatus for ionizing a substance to be analyzed in a solution, the electrospray ionization apparatus being usable as a component of a mass spectrometry system such as a mass spectrometer;
  • the electrospray ionization device comprises: an ionization chamber 1, a liquid suction head 2, an air guiding tube 3, an ion sampling port 4, and the like.
  • the liquid tip 2 is tubular, and the solution may be sucked from one end (for example, the upper end of the drawing) of the liquid tip 2 by means of a pipetting gun, or may be injected from the opposite end (for example, the lower end of the figure).
  • the liquid tip 2 is used to guide the solution into the ionization chamber 1 from the outlet end (lower end in the drawing) of the liquid tip 2 in the form of droplets.
  • the liquid tip 2 is preferably a liquid tip (ie, a disposable tip), wherein at least a portion of the liquid tip 2 maintains a potential difference with respect to the surface of the ionization chamber 1 for a predetermined time to allow entry.
  • the droplet of the ionization chamber 1 is charged; in the embodiment, the potential difference is a DC high voltage potential difference, and the applied DC high voltage potential difference can be directly applied to the tip of the liquid tip, or can be applied to the tip of the tip through a dielectric, and the dielectric can be The outer wall of the air or the insulating tip or other dielectric material; the DC high voltage potential difference ranges from 1000 to 3000V; or 3000 to 5000V; or 5000 to 8000V; or 8000 to 12000V; in addition, the DC high voltage potential
  • the suction head 2 is engaged through a high resistance (e.g., 10 M [Omega) resistance of the liquid.
  • the material of the liquid tip 2 includes one of a metal, a quartz tube, an organic polymer material, and glass. A DC or AC power is applied to the liquid tip 2 of the metal material as a preferred solution.
  • the air ducts 3 may be one or more in number for guiding one or more air streams into the ionization chamber 1 , the air stream being directed to the outlet end of the liquid tip 2 .
  • the function of the air flow of the air guiding tube 3 is described below: generally, the outer diameter of the outlet end (or the tip end) of the liquid suction head used in the prior art is generally larger than 350 ⁇ m, and the outlet of the outer diameter of 350 ⁇ m can be applied by applying a voltage of more than 4 kV to the suction head. Produces a beam of electrospray at the end, however its spray stability is poor, and as the liquid flow rate increases, The spray stability is gradually reduced.
  • the air current is blown to the outlet end of the liquid suction head 2, and at this time, it is possible to apply, for example, a suction head outlet having an outer diameter of 350 ⁇ m or even 750 ⁇ m without applying electricity.
  • a stable spray is generated at the end, and a voltage greater than 2.0 kV is applied to the tip of the liquid tip 2 to convert the spray into an electrospray, which in turn produces a stable ion current within the ion analyzer.
  • the auxiliary atomizing gas and the liquid conduit are usually placed coaxially, and the gap between the liquid conduit and the auxiliary atomizing gas conduit is small, which makes it difficult to achieve instant separation between the liquid conduit and the auxiliary atomizing gas conduit;
  • the instantaneous separation of the liquid conduit from the auxiliary atomizing gas conduit can be achieved, but it is difficult to avoid contamination of the gas conduit for atomization by the liquid in the tube when the liquid conduit is replaced; in this embodiment, the side blowing airflow is used.
  • the liquid suction head 2 and the air guiding tube 3 can be instantly separated, which enables the liquid suction head 2 to be frequently replaced without contaminating the air guiding tube 3, thereby avoiding mutual interference between multiple analyses.
  • the inner diameter of the air guiding tube 3 is preferably 0.5 mm to 2 mm, and the gas flow rate can be adjusted by a manual or computer controlled manner, for example, by a pressure valve.
  • the air flow speed ranges from 10 to 100 m / s; or 100 ⁇ 300 m / s; or 300 ⁇ 600 m / s; or 600 ⁇ 800 m / s, preferably, the air flow rate is controlled between 100 to 200 m / s.
  • pressure control may be used alone or in combination, for example:
  • the solution passes through the liquid tip 2 under the pressure difference applied between the inlet end and the outlet end of the liquid tip 2, wherein the pressure at the inlet end of the liquid tip 2 is stronger than the pressure at the outlet end of the liquid tip 2.
  • the airflow sent by the air guiding tube 3 passes through the outlet end of the liquid suction head 2 to form a pressure difference between the inlet end of the liquid suction head 2 and the outlet end of the liquid suction head 2; the solution can also be passed under the pressure difference
  • the liquid tip 2 preferably, the pressure difference is generated, for example, such that the inlet end of the liquid tip 2 is connected to atmospheric pressure, and the pressure at the outlet end of the liquid tip 2 forms a negative pressure due to the action of the gas flow.
  • the formation of the electrospray can be achieved, for example, by forming a pressure difference between the outlet end of the liquid tip 2 and the inlet end, so that the liquid in the tip can flow out from the tip, and the discharged liquid can be electrosprayed under the action of voltage and gas flow.
  • a rapidly moving gas stream flowing through the tip of the liquid tip 2 can reduce the static pressure at the outlet end of the liquid tip 2 by the jet effect, and the other end of the liquid tip 2, that is, the inlet end can be, for example, At atmospheric pressure, under the action of the pressure difference between the two ends of the liquid tip 2, the liquid therein is pushed out, and the discharged liquid can also be electrosprayed under the action of voltage and air flow.
  • the gas may be heated in the air guiding tube 3, and the heated gas contributes to desolvation of the spray droplets.
  • the air guiding tube 3 and the liquid suction head 2 extend at a predetermined angle; optionally, the angle between the air guiding tube 3 and the liquid suction head 2 ranges from 20° to 60°. Or 60 ° ⁇ 90 °; or 90 ° ⁇ 120 °, in order to obtain better auxiliary spray efficiency, 70 ° ⁇ 85 ° is a more preferred solution.
  • the open end of the ion sampling port 4 faces the ionization chamber 1 and is used to guide the analyte ions of the solution after the ion detachment liquid tip 2 enters the ionization chamber 1 in the direction indicated by the arrow in the figure. Entering the electrospray ionization
  • the quality analyzer of the lower stage of the device is used to complete the mass analysis; the angle between the ion sampling port 4 and the direction of the air flow may vary from 0° to 90°, in order to reduce the contamination of the ion analyzer by the spray droplets, 45° to 90° is a more preferred solution.
  • an electrospray ionization apparatus in accordance with one embodiment of the present invention is shown.
  • the potential difference is an alternating current potential difference, and the positive and negative ions are alternately generated with the periodic change of the voltage;
  • the applied alternating current potential difference can be directly applied to the liquid suction head 2; or can be applied to the liquid suction head 2 through the dielectric.
  • the dielectric can be air, an insulated outer wall of the tip or other dielectric material.
  • the optional frequency of the alternating current potential difference is 2 to 1000 Hz, preferably a high frequency of 5 Hz to 50 Hz; optionally, the peak-to-peak value of the alternating high voltage potential ranges from 1000 to 3000 V; or 3000 to 5000 V; 5000 to 8000 V; or 8,000 to 12,000 V, preferably 6 kV to 10 kV.
  • the liquid suction head 5 is a conical liquid suction head having a tapered tip as the outlet end, and the liquid suction head 5 can be made of an organic polymer material, and an electrospray is realized by using an alternating high voltage. .
  • the alternating high voltage is applied to the tip of the tip through the dielectric of the tip wall. Since the electrode to which the voltage is applied is not in contact with the solution, this embodiment can avoid interference of the analysis of the solution remaining on the electrode in the process of replacing the liquid tip.
  • the outer diameter of the tapered tip of the liquid tip 5 ranges from 10 to 50 ⁇ m; or from 50 to 200 ⁇ m; or from 200 to 500 ⁇ m; or from 500 to 1000 ⁇ m; or from 1000 to 2000 ⁇ m.
  • FIG. 4 another embodiment of the present invention which is based on the embodiment of Fig. 3 is shown.
  • the outer diameter of the tip of the liquid tip 5 is larger than 750 ⁇ m and the inner diameter is larger than 350 ⁇ m, since the flow resistance of the liquid is too small, the liquid flows out from the tip of the tip placed vertically under the action of its own weight, and even if the auxiliary airflow is used, it cannot be obtained.
  • Stable spray By filling a certain amount of the porous chromatography material 6 having good liquid permeability in the liquid tip 5, the flow resistance of the liquid can be increased to achieve stable electrospray under the aid of gas.
  • the chromatographic packing 6 can be used for the removal of impurities and target analytes in complex sample analysis; the materials used for the chromatographic packing 6 include: porous silica particles, paper fiber porous monoliths, polymer monoliths and polymers Wrapped in one or more mixtures of particles.
  • the present invention forms an electrospray by a combination of a gas flow and a voltage, so that the action of the gas flow can effectively reduce the requirement for lowering the voltage applied to the liquid tip 5.
  • the outer diameter of the tip of the tip is greater than 750 ⁇ m
  • the alternating high voltage is applied to the tip of the tip through the dielectric of the tip wall.
  • a higher voltage is required to produce a stable electrospray at the tip of the tip, which can result in severe discharge; in the embodiment of the invention, when there is an auxiliary gas, the exchange is applied.
  • the peak-to-peak value of the voltage is greater than 8kV.
  • Figure 5 shows the mass spectrum of 50 ng/mL phenformin and rosiglitazone in methanol after detection on the apparatus of the present invention (horizontal to mass-to-charge ratio, vertical axis is ion current intensity).
  • the mass spectrometer operates in positive ion mode.
  • the liquid tip used was a polypropylene plastic pipette tip with a tip outer diameter of 350 ⁇ m.
  • the solution can be directly absorbed using a pipette equipped with the liquid tip, or Inject from the other end of the tip of the liquid tip.
  • the applied DC voltage was 3.0 kV and was directly contacted with the solution through a 10 M ⁇ resistor.
  • the auxiliary gas flow rate used was 5 L/min.
  • Figure 6 shows the mass spectrum of 50 ng/mL phenformin and rosiglitazone in methanol after detection on the apparatus of the present invention.
  • the mass spectrometer operates in positive ion mode.
  • the liquid tip used was a polypropylene plastic pipette tip with a tip outer diameter of 350 ⁇ m.
  • the solution can be directly aspirated using a pipette equipped with the tip or from the other end of the tip of the liquid tip.
  • the applied DC voltage is a square wave AC voltage ( ⁇ 3.0kV, 50% duty cycle, 5Hz), and is directly in contact with the solution through a 10M ⁇ resistor.
  • the auxiliary gas flow rate used was 5 L/min.
  • Figure 7 shows the mass spectrum of 50 ng/mL phenformin and rosiglitazone in methanol after detection on the apparatus of the present invention.
  • the mass spectrometer operates in positive ion mode.
  • the liquid tip used was a polypropylene plastic pipette tip with a tip outer diameter of 350 ⁇ m.
  • the solution can be directly aspirated using a pipette equipped with the tip or from the other end of the tip of the liquid tip.
  • the applied AC voltage is a square wave AC voltage ( ⁇ 3.0 kV, 50% duty cycle, 5 Hz), applied to the tip of the tip by a layer of conductive metal coated on the outer wall of the tip.
  • the auxiliary gas flow rate used was 5 L/min.
  • Figure 8 shows the mass spectrum of 50 ng/mL phenformin and rosiglitazone in methanol after detection on the apparatus of the present invention.
  • the mass spectrometer operates in positive ion mode.
  • the liquid tip used was a quartz capillary having an outer diameter of 300 ⁇ m.
  • the solution is inhaled by capillary siphoning.
  • the applied AC voltage is a square wave AC voltage ( ⁇ 3.0 kV, 50% duty cycle, 5 Hz), applied to the tip of the tip by a layer of conductive metal coated on the outer wall of the capillary.
  • the auxiliary gas flow rate used was 5 L/min.
  • Figure 9 shows the mass spectrum of phenformin and rosiglitazone in a urine sample after detection on the apparatus of the present invention.
  • the tip used was a commercial 20 ⁇ L polypropylene plastic pipette tip with an outer diameter of about 750 ⁇ m and 5 ⁇ L of chromatographic silica gel pre-blocked in the tip.
  • the spiked concentration of the urine sample used was 200 ng/mL.
  • 2 ⁇ L of the spiked urine sample was pipetted from the opposite end of the tip of the tip to the surface of the chromatographic silica gel, and then analyzed by 100 ⁇ L of acetonitrile-water (8:2) solution.
  • the applied AC voltage is a square wave AC voltage ( ⁇ 4.0 kV, 50% duty cycle, 5 Hz) through a conductive metal layer coated on the outer wall of the tip and applied to the tip of the tip.
  • the auxiliary gas flow rate used was 5 L/min.
  • the above embodiments are merely illustrative of the possibilities of the present invention, and those skilled in the art can conveniently design various implementation configurations under the framework of the present invention.
  • the relative positions of the liquid suction head, the gas conduit and the ion introduction port are adjustable to meet the needs of different sizes of tips and different gas flow rates;
  • the shape of the gas conduit outlet can be not only circular but also polygonal, arc Shape or other shape.
  • the electrospray ionization apparatus of the present invention can be applied to a mass spectrometry system such as a mass spectrometer, which can include the electrospray ionization apparatus of the present invention, and a mass analyzer as a lower stage thereof.
  • a mass spectrometry system such as a mass spectrometer, which can include the electrospray ionization apparatus of the present invention, and a mass analyzer as a lower stage thereof.
  • the present invention provides an electrospray ionization device for ionizing a solution to be analyzed, the electrospray ionization device comprising: an ionization chamber; and a liquid tip for guiding the solution to a droplet Forming from the outlet end of the liquid tip into the ionization chamber, at least a portion of the liquid tip maintaining a potential difference relative to the surface of the ionization chamber for a predetermined time to charge the droplet entering the ionization chamber; Or a plurality of air guiding tubes for guiding one or more air currents into the ionization chamber, the one or more air currents directed to an outlet end of the liquid suction head, and an extending direction of the air guiding tube and the liquid suction head Forming a predetermined angle; wherein the air guiding tube and the liquid suction head are disposed to be freely separable; the ion sampling port has an open end facing the ionization cavity, and the ion to be analyzed for guiding the solution enters

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Abstract

Cette invention concerne un appareil d'ionisation par électronébuliseur conçu pour ioniser la substance à analyser dans une solution, comprenant une chambre d'ionisation (1), un élément d'aspiration de liquide, une ou plusieurs conduites d'air (3) et un orifice d'échantillonnage d'ions (4). L'élément d'aspiration de liquide (2) introduit la solution à partir de l'extrémité de sortie de l'élément d'aspiration de liquide (2) à l'intérieur de la chambre d'ionisation (1) en forme de gouttelettes, et une différence de potentiel est maintenue pendant une période prédéterminée entre au moins une partie de l'élément d'aspiration de liquide (2) et la surface de la chambre d'ionisation (1) de manière à charger les gouttelettes. Ladite/lesdites conduites d'air (3) introduisent un ou plusieurs flux d'air vers l'extrémité de sortie de l'élément d'aspiration de liquide (2) dans la chambre d'ionisation (1), et le sens de déplacement des flux d'air est à un angle prédéterminé par rapport au sens d'extension de l'élément d'aspiration de liquide (2). Les conduites d'air (3) et l'élément d'aspiration de liquide (2) sont librement détachables. L'extrémité d'ouverture de l'orifice d'échantillonnage d'ions (4) est orientée vers la chambre d'ionisation (1) pour introduire les ions de la substance à analyser dans la solution dans le spectromètre de masse au niveau de l'étage suivant de l'appareil d'ionisation par électronébuliseur. Il est ainsi possible d'obtenir un appareil d'ionisation présentant une analyse de masse directe, rapide, une bonne reproductibilité et sans contamination croisée, et l'ionisation par électronébuliseur peut être effectuée sur l'élément d'aspiration jetable présentant une plus grande dimension d'extrémité de pointe sans que l'électrode entre en contact avec la solution.
PCT/CN2015/092690 2014-10-27 2015-10-23 Appareil d'ionisation par électronébuliseur et spectromètre de masse WO2016066062A1 (fr)

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CN201410581596.9A CN105632866B (zh) 2014-10-27 2014-10-27 电喷雾电离装置及质谱仪
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GB201807914D0 (en) * 2018-05-16 2018-06-27 Micromass Ltd Impactor spray or electrospray ionisation ion source
CN109326499B (zh) * 2018-09-29 2020-06-02 清华大学深圳研究生院 一种用于去除质谱仪进样结晶的装置
CN109360781B (zh) * 2018-11-29 2019-11-26 清华大学深圳研究生院 二次电喷雾离子源装置及质谱检测设备
EP4006953A1 (fr) 2020-11-27 2022-06-01 F. Hoffmann-La Roche AG Positionnement d'un composant aciculaire dans un système de spectrométrie de masse
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