WO2015192330A1 - 核酸提取设备及方法 - Google Patents
核酸提取设备及方法 Download PDFInfo
- Publication number
- WO2015192330A1 WO2015192330A1 PCT/CN2014/080129 CN2014080129W WO2015192330A1 WO 2015192330 A1 WO2015192330 A1 WO 2015192330A1 CN 2014080129 W CN2014080129 W CN 2014080129W WO 2015192330 A1 WO2015192330 A1 WO 2015192330A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extract storage
- nucleic acid
- capping
- storage container
- acid extraction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Definitions
- a nucleic acid extraction device and method of the invention A nucleic acid extraction device and method of the invention.
- Nucleic acid extraction technology mainly refers to separating nucleic acids from biological macromolecules such as proteins, polysaccharides, and fats for detection.
- the nucleic acid extraction product is dispensed into an output container for subsequent operations.
- a 96-well plate is usually used as an output container for the proposed product for subsequent PCR (ie, polymerase chain reaction) or other nucleic acid test operations, and in order to prevent solution spillage and evaporation in the well, it is necessary to seal the 96-well plate. .
- the nucleic acid is exponentially expanded, the sensitivity of the test is high, and the measurable linear range is high, which makes the technique extremely sensitive to cross-contamination between samples.
- a nucleic acid extraction apparatus comprising:
- An extraction device for extracting a nucleic acid extraction product from the sample solution; and a dispensing device for dispensing the nucleic acid extraction product into the extract storage container;
- an extract storage device configured to store an extract storage container having a plurality of independent small holes, wherein the separate small holes respectively store the final nucleic acid extraction product;
- a capping device for covering the cover on the extract storage container
- the moving mechanism is configured to drive the capping device and the extract storage device to move relatively when the extract storage container satisfies the capping condition, so that the capping device covers the capping material on the extract storage container.
- a nucleic acid extraction method comprising the steps of: extracting a final nucleic acid extraction product from a sample solution;
- the drive capping device and the extract storage device for placing the extract storage container are relatively moved, and the extract storage container is sealed with the cap without leaving the extract storage device. Live the extract storage container.
- the nucleic acid extraction device and the extraction method of the present invention when the extract storage container satisfies the capping condition, the relative movement of the extract storage device and the capping device is performed without the extract storage container leaving the extract storage device, The final nucleic acid extraction product on the extract storage container is sealed with a closure, and the entire extract storage container is sealed and then taken out for subsequent operations. Therefore, after the dispensing and before the capping, the extract storage container is not manually moved to avoid cross-contamination of the liquid during manual movement.
- 1 is a schematic view showing the layout of an embodiment of the nucleic acid extraction device of the present application.
- FIG. 2 is a schematic view showing a sealing film of an extract storage container in an embodiment of the present application
- FIG 3 is a partial structural view of a film sealing device in an embodiment of the present application.
- the first embodiment provides a nucleic acid extraction device 1 .
- the nucleic acid extraction device 1 includes: a sample holder 2 , an elution device 3 , a nucleic acid tip (T IP head) frame 4 , a dispensing device 5 , and an extraction method.
- the sample holder 2 is used to store a sample solution container.
- the incubation device is used to complete the incubation operation.
- the nucleic acid tip holder 4 is provided with a disposable tip.
- the sample addition mechanism, reagent addition mechanism, and eluent addition mechanism are used to add samples, reagents, and eluents, respectively.
- the separation device performs a magnetic separation operation for separating a nucleic acid-binding carrier (e.g., magnetic beads) to which nucleic acid is adsorbed from the solution.
- An elution device is used to detach the nucleic acid from the nucleic acid binding vector to form a nucleic acid extraction product.
- the nucleic acid may not be detached from the nucleic acid tuberculosis carrier, and the magnetic beads may be directly detected.
- the apparatus for extracting the nucleic acid extraction product from the sample solution in the nucleic acid extraction apparatus is collectively referred to as an extraction apparatus.
- the dispensing device 5 is for injecting the nucleic acid extraction products produced by the elution device 3 into the extraction separately The contents are stored in each of the individual small holes in the container.
- the extract storage device 6 is used for accommodating and positioning an extract storage container, which may be a 96-well plate, a 384-well plate, and other components commonly used as an extract storage container in nucleic acid extraction in the art.
- an extract storage container which may be a 96-well plate, a 384-well plate, and other components commonly used as an extract storage container in nucleic acid extraction in the art.
- the 96-well plate is exemplified below.
- 96-well plate 8 (also known as deep-well plate) is an extract storage container commonly used in the field, which is usually arranged on a flat plate with 8x 12 independent small holes. 81.
- the size of the plate, the position and size of the holes are usually fixed specifications.
- the capping device 7 is used to cover the entire extract storage container, which includes integrally covering the 96-well plate 8 with a sealing film, and also includes capping each of the individual small holes 81.
- the capping device 7 of the present embodiment 1 includes a take-up roll 71 and a cutting mechanism (not shown).
- the roll 71 is used to place a film roll 9 (a roll-shaped sealing film) and to unwind.
- the moving mechanism drives the capping device 7 and the 96-well plate 8 to move relative to each other, and covers the cover plate on the 96-well plate 8.
- the relative movement of the capping device 7 and the 96-well plate 8 includes the following three modes:
- the capping device 7 moves, the extract storage device 6 (the 96-well plate 8 placed on the opposite side of the extract storage device 6 is stationary);
- the extract storage device 6 (the 96-well plate 8 placed thereon is stationary relative to the extract storage device 6) moves, and the capping device 7 does not move;
- the capping device 7 and the extract storage device 6 (the 96-well plate 8 placed thereon are stationary with respect to the extract storage device 6) move.
- the moving mechanism can also be connected to the dispensing device 5, and the dispensing device 5 is driven to perform the dispensing operation in the dispensing operation.
- the cover includes a sealing film 91 and a sealing cover, and the sealing film 91 further includes a common sealing film 91 and a magnetic film (also called a magnetic film).
- the sealing film 91 (both a normal film and a magnetic film) can be exemplified, and the capping device ⁇ further includes a pressure roller 72 and a nip roller 73.
- the pressing roller 72 is used as a pressing member.
- the sealing film 91 is covered on the 96-well plate 8
- the pressing roller 72 is positioned above the 96-well plate 8, and the sealing film 91 is pressed against the 96-well plate 8, and the relative horizontal movement process is performed. Achieve a seal.
- the nip roller 73 and the pressure roller 72 cooperate with each other, and the sealing film 91 passes between the press roller ⁇ 2 and the nip roller 73 to provide tension for unwinding of the sealing film 91.
- the nip roller 73 is only guaranteed The sealing film 91 is unwound more smoothly, and in other embodiments, the nip roller 73 can also be omitted.
- the moving mechanism may include a horizontal moving mechanism and a vertical moving mechanism, and the moving mechanism may use a mechanism commonly used to drive the movement of other components, such as a pneumatic, hydraulic, electric, electromagnetic driving, etc., and the moving mechanism may also Including the guiding member to guide the moving direction, such as the guide post, the guide groove and the like.
- the horizontal moving mechanism drives the capping device 7 to move horizontally to perform capping, such as the moving roller 71, the nip roller 73 and the pressing roller 72-to perform horizontal movement.
- the capping device 7 is located above the 96-well plate 8 and gives a certain distance upward, so that it is required to be moved downward by the vertical moving mechanism when the film is sealed, so that the pressing roller 72 can press the sealing film 91. Close to the 96-well plate 8.
- the vertical moving mechanism can only drive the nip roller 73 and the pressure roller 72 to move up and down, and the material roller 71 remains stationary, and can also drive the material roller 71, the nip roller 73 and the pressure roller 72 to move up and down in the vertical direction.
- the vertical movement mechanism may also be an unnecessary feature, for example, the pressure roller 72 and the nip roller 73 may be set to be constant with respect to the 96-hole plate 8, when the pressure roller 72 and the nip roller 73 are horizontally moving.
- the vertical movement mechanism is non- Necessary features.
- the 96-well plate 8 can be made of a weak magnetic material, and when the magnetic film covers the 96-well plate 8, the magnetic film is automatically adsorbed on the 96-well plate 8 Above, therefore, in the embodiment using the magnetic film, it is possible to choose to dispense with the lower pressing member.
- the cutting mechanism is used for cutting the sealing film 91 capped on the 96-well plate 8 from the film roll after the sealing film 91 seals the 96-well plate 8, and can be used for various types of commonly used tape cutting. Cutting mechanism.
- the capping device ⁇ may be provided with a heating mechanism for heating the cover to achieve heat sealing, wherein the heating mechanism may be disposed on the press roller 72 or the nip roller 73.
- the sample solution is placed on the sample holder 2, and then passed through the components on the nucleic acid extraction device 1 (such as an incubation device, a sample addition mechanism, a reagent addition mechanism, a separation device, an elution device, an eluent addition mechanism, etc.)
- the nucleic acid extraction product is formed at the elution device 3 after the extraction of the nucleic acid in the sample solution is completed by a series of operations such as incubation, separation, elution, and the like.
- the dispensing nozzle 5 loads the disposable tip at the nucleic acid tip holder 4, and then the nucleic acid
- the extracted product is taken up from the elution device 3, and then dispensed into a 96-well plate 8 placed in the extract storage device 6, and the reagents required for the subsequent tests are added thereto.
- the sealing device 7 completes the sealing of the 96-well plate 8. Therefore, after the dispensing and before the capping, the 96-well plate 8 is not moved, and there is no cross-contamination of the liquid during the movement due to the unsealed 96-well plate 8.
- the second embodiment provides another capping device
- the capping device includes a picking member for picking up a pre-cut sealing film
- the moving mechanism drives the picking member.
- the picked sealing film was entirely covered and pressed on a 96-well plate.
- the pickup member may be provided with a flat plate having a size matched with a 96-well plate, and a plurality of suction cups are provided on the flat plate for adsorbing the sealing film.
- the sealing film prepared according to the size of the 96-well plate is sent to the corresponding position of the pickup by an external third-party component, and is directly adsorbed by the pickup. That is, in this mode, the moving mechanism only needs to drive the pickup to complete the vertical movement.
- Third-party components can be robots, suction cups or electrostatic adsorption.
- the capping device is driven by the moving mechanism to pick up the cut sealing film from the area where the sealing film is stored, and then return to seal.
- the moving mechanism may include a horizontal moving mechanism and a vertical moving mechanism, the horizontal moving mechanism drives the pickup member to move horizontally, and the vertical moving mechanism drives the pickup member to move in the vertical direction.
- the moving mechanism can use the existing mechanisms that can drive the movement of other components, such as pneumatic, hydraulic, electric, electromagnetic driving, etc., and the moving mechanism can also include guiding members to guide the moving direction, such as the guide column, Guide groove, etc.
- the cover used in the embodiment is a sealing cover, that is, a cover adapted to a separate small hole in the 96-hole plate.
- the capping device comprises a picking member, and the moving mechanism drives the picking member to move between the sealing cap storage position and the 96-hole plate to pick up and place the sealing cap.
- a pressing member may be further included. After the sealing member is placed on the sealing cover, the moving mechanism drives the pressing member to move to the 96-hole plate, and the pressing member presses the sealing cover downward to make the sealing cover and the sealing cover Corresponding to the independent small hole cover.
- the sealing cover picked up by the picking member may be a cover corresponding to a separate small hole. It may also be a cover of an integral structure corresponding to a plurality of independent small holes according to the distribution of small holes on the 96-well plate, such as for an 8-piece cap or a 96-well cap.
- Pick-up parts can be selected with a pick-up function such as a robot, and the press-fit parts can be selected from a 96-hole plate size or the like.
- This embodiment 4 provides a nucleic acid extraction method comprising the steps of:
- the final nucleic acid extraction product is extracted from the sample solution.
- the final nucleic acid extraction product is extracted by nucleic acid extraction techniques such as incubation, magnetic separation, and elution, and is used for subsequent detection.
- the extracted final nucleic acid extraction product is separately injected into the extract storage container.
- the extract storage container may be a 96-well plate, a 384-well plate, or other components commonly used as an extract storage container in nucleic acid extraction in the art.
- a 96-well plate will be exemplified hereinafter.
- the method further includes:
- the relative movement of the capping device and the 96-well plate includes the movement of the capping device, the extract storage device (the 96-well plate placed thereon is stationary relative to the extract storage device), or the extract storage device (the 96 placed thereon)
- the orifice plate moves relative to the extract storage device, and the closure device does not move; or the closure device and the extract storage device (the 96-well plate placed thereon is stationary relative to the extract storage device) are moved.
- the 96-well plate is not manually moved to avoid cross-contamination of the liquid during manual movement.
- the extract storage device can be kept stationary, and the capping device is moved relative to the extract storage device to perform capping, so that the capping device is directly capped without moving the position of the 96-well plate. It can further avoid liquid cross-contamination caused by moving the 96-well plate process (ie including manual movement, including mechanical movement).
- the closure can be sealed with a sealing film or a sealing cover.
- the sealing device as shown in Embodiments 1 to 3 can be used to seal the independent small holes in the 96-well plate, and then move 96 after being sealed. Orifice plate.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
一种核酸提取设备及提取方法中，在提取物存放容器满足封盖条件时，由于在提取物存放容器不离开提取物存放装置的情况下，通过提取物存放装置和封盖装置的相对移动，用封盖物密封住提取物存放容器上的最终核酸提取产物，待整个提取物存放容器被密封好后再将其取出，以待后续操作。因此，在分注后、封盖前，并不手动移动提取物存放容器，避免手动操作存在的不稳定因素导致液体的交叉污染。
Description
核酸提取设备及方法
技术领域
本发明一种核酸提取设备及方法。
背景技术
核酸提取技术主要是指将核酸与蛋白质、 多糖、 脂肪等生物大分子 物质分开， 以用于检测。
现有自动化核酸提取设备中， 在将待测样本中的核酸提取完成形成 最终的核酸提取产物后， 将核酸提取产物分注到输出容器中进行后续操 作。通常釆用 96孔板作为提出产物的输出容器，进行后续的 PCR (即聚 合酶链式反应） 或其他核酸测试操作， 而为了防止孔中的溶液泼洒及蒸 发， 所以需要对 96孔板进行密封。
在后续测试环节， 特别是常用的实时 PCR技术对核酸进行指数扩 增， 其测试灵敏度很高， 且可测量的线性范围很高， 由此使得该技术对 样本间的交叉污染异常敏感。
但现有的自动化核酸提取设备通常在完成整个 96 孔板分注提出产 物后， 由操作人员手工取出 96孔板， 移动至封膜设备进行封膜， 但由于 96孔板每个孔的深度浅， 而且孔与孔之间的间距很小， 而在搬运过程中 不可避免会造成孔内的提取产物溅入周围的孔位间而导致交叉污染， 使 得该环节的交叉污染风险较高且处于不可控的状态。
发明内容
依据本发明的一方面提供一种核酸提取设备， 包括：
提取装置， 用于从样本溶液中提取核酸提取产物； 分注装置， 用 于将核酸提取产物分注至提取物存放容器内；
提取物存放装置， 所述提取物存放装置用于安放具有多个独立小孔 的提取物存放容器， 所述独立小孔内分别存放最终核酸提取产物；
还包括：
封盖装置， 用于将封盖物覆盖在提取物存放容器上；
移动机构， 用于在提取物存放容器满足封盖条件时驱动封盖装置和 提取物存放装置产生相对移动， 以使封盖装置将封盖物覆盖在提取物存 放容器上。
依据本发明的另一方面提供一种核酸提取方法， 其包括步骤： 从样本溶液中提取得到最终核酸提取产物；
将提取的最终核酸提取产物分别注入到提取物存放容器的独立小孔
内；
还包括：
在提取物存放容器满足封盖条件时驱动封盖装置和用于安放提取物 存放容器的提取物存放装置产生相对移动， 在提取物存放容器不离开提 取物存放装置的情况下用封盖物密封住提取物存放容器。
依据本发明的核酸提取设备及提取方法， 在提取物存放容器满足封 盖条件时， 在提取物存放容器不离开提取物存放装置的情况下， 通过提 取物存放装置和封盖装置的相对移动， 用封盖物密封住提取物存放容器 上的最终核酸提取产物，待整个提取物存放容器被密封好后再将其取出， 以待后续操作。 因此， 在分注后、 封盖前， 并不手动移动提取物存放容 器， 避免手动移动过程中导致液体的交叉污染。
附图说明
图 1是本申请核酸提取设备一种实施例布局示意图；
图 2是本申请一种实施例中提取物存放容器封膜示意图；
图 3是本申请一种实施例中封膜装置部分结构示意图。
具体实施方式
实施例 1 :
请参考图 1 ,本实施例 1提供一种核酸提取设备 1 ,该核酸提取设备 1包括： 样本架 2、 洗脱装置 3、 核酸吸头 （T IP头） 架 4、 分注装置 5、 提取物存放装置 6、 封盖装置 7、 移动装置（未示出 )以及其他部件， 其 他部件如孵育装置、 样本添加机构、 试剂添加机构、 分离装置、 洗脱液 添加机构等。
样本架 2用于存放样本溶液容器。 孵育装置用于完成孵育操作。 核 酸吸头架 4则是提供一次性吸头。 样本添加机构、 试剂添加机构和洗脱 液添加机构分别用于添加样本、 试剂和洗脱液。 分离装置进行磁分离操 作， 用于将吸附有核酸的核酸结合性载体（如磁珠）从溶液中分离出来。 洗脱装置用于使核酸从核酸结合性载体上脱离开来，形成核酸提取产物。 其中， 在其他实施例中， 也可不将核酸与核酸结核性载体脱离， 直接带 磁珠进行检测。
无论核酸提取产物最后以何种形式进行检测， 本核酸提取设备中将 实现从样本溶液中提取分离核酸提取产物这一过程的装置统称为提取装 置。
分注装置 5用于将洗脱装置 3产生的核酸提取产物分别注入到提取
物存放容器内的每个独立小孔中。
提取物存放装置 6用于安放和定位提取物存放容器， 该提取物存放 容器可以为 96孔板、 384孔板以及本领域在核酸提取中常用作提取物存 放容器的其他部件， 为了便于描述， 下文中均以 96孔板为例进行说明。
请参考图 2 , 96孔板 8 (也可称为深孔板） 是当前该领域内较常使 用的一种提取物存放容器， 其通常为一个平板上均勾排布 8x 12 个独立 小孔 81。 其中板的尺寸、 孔的位置及尺寸通常为固定的规格。
封盖装置 7用于对整个提取物存放容器进行封盖， 此处所述封盖包 括用密封膜对 96孔板 8进行整体封盖， 也包括对每个独立小孔 81进行 加盖密封。
请参考图 3 ,本实施例 1所提供的封盖装置 7包括料辊 71和裁切机 构 （未示出）。 该料辊 71用于放置膜卷料 9 (卷料状的密封膜） 并进行 放卷。
移动机构带动封盖装置 7和 96孔板 8相对移动， 将封盖物覆盖在 96孔板 8上。 其中， 封盖装置 7和 96孔板 8相对移动包括以下三种方 式：
1、 封盖装置 7移动， 提取物存放装置 6 (其上放置的 96孔板 8相 对提取物存放装置 6静止） 不动；
2、提取物存放装置 6 (其上放置的 96孔板 8相对提取物存放装置 6 静止） 移动， 封盖装置 7不动；
3、 封盖装置 7和提取物存放装置 6 (其上放置的 96孔板 8相对提 取物存放装置 6静止） 都移动。
另外， 该移动机构也可与分注装置 5连接， 在分注操作中驱动分注 装置 5完成分注工作。
该封盖物包括密封膜 91和密封盖， 密封膜 91又包括普通密封膜 91 和磁性膜 （又叫磁性薄膜）。
请继续参考图 3 , 具体地， 本实施例 1以釆用密封膜 91 (普通膜和 磁性膜均可）为例， 该封盖装置 Ί还包括压辊 72和夹辊 73。 压辊 72作 为下压件， 当密封膜 91覆盖在 96孔板 8上时， 压辊 72位于 96孔板 8 的上方， 将密封膜 91压紧在 96孔板 8上， 在相对水平移动过程中实现 密封。 同时， 夹辊 73和压辊 72相互配合， 密封膜 91从压辊 Ί 2与夹辊 73之间穿过， 可为密封膜 91的放卷提供张力。 但是， 夹辊 73只是保证
密封膜 91放卷更加顺畅， 在其他实施例中， 也可省去夹辊 73。
移动机构可以包括水平移动机构和竖直移动机构， 移动机构均可釆 用现有常用可实现驱动其他部件移动的机构， 如气动、 液动、 电动、 电 磁驱动等驱动机构， 同时移动机构也可以包括导向件对移动方向进行导 向， 如导柱、 导槽等。
水平移动机构带动封盖装置 7水平移动进行封盖， 如带动料辊 71、 夹辊 73和压辊 72—同进行水平移动。
本实施例 1中，封盖装置 7位于 96孔板 8的上方，并且向上让出一 定的距离， 因此在封膜时需要通过竖直移动机构下移，使压辊 72能够将 密封膜 91压紧在 96孔板 8上。 而其中竖直移动机构可以仅带动夹辊 73 和压辊 72上下移动， 料辊 71保持不动， 也可以带动料辊 71、 夹辊 73 和压辊 72—同在竖直方向上下移动。
当然， 在其他实施例中， 竖直移动机构也可以为非必要特征， 例如 压辊 72和夹辊 73相对 96孔板 8高度可设置成一定， 当压辊 72和夹辊 73在水平移动机构带动下移动到 96孔板 8上时， 压辊 72 已经紧贴 96 孔板 8上， 可以将密封膜 91压紧在 96孔板 8上， 在此实施例中， 竖直 移动机构则为非必要特征。
另外， 当釆用磁性膜时， 由于磁性膜具有磁吸性， 96孔板 8可选用 弱磁性材料制成， 当磁性膜覆盖到 96孔板 8上时， 磁性膜自动吸附在 96孔板 8上， 因此， 在使用磁性膜的实施例中， 是可以选择省去下压件 的。
该裁切机构是用于在密封膜 91密封 96孔板 8后将封盖于 96孔板 8 上的密封膜 91从膜卷料上裁切掉， 可釆用现有各类常用带料裁切机构。
另外， 对于需要进行热封的 96孔板 8 , 封盖装置 Ί可以增设加热机 构， 用于对封盖物进行加热实现热封， 其中加热机构可设置在压辊 72 或夹辊 73上。
本实施例过程可简述如下：
请参考图 1 ,样本溶液放置在样本架 2上， 然后通过核酸提取设备 1 上的部件 （如孵育装置、 样本添加机构、 试剂添加机构、 分离装置、 洗 脱装置、 洗脱液添加机构等） 通过一系列的操作 （如孵育、 分离、 洗脱 等） 将样本溶液中的核酸提取完成后， 在洗脱装置 3处形成核酸提取产 物。 此时由分注装置 5在核酸吸头架 4处装载一次性吸头， 然后将核酸
提取产物从洗脱装置 3吸取后分注到提取物存放装置 6内放置的 96孔板 8中， 并向其中加入后续测试所需的试剂。 在一个 96孔板 8完成加样或 部分完成加样后， 当满足封盖条件， 即保持 96孔板 8不动， 由封盖装置 7对 96孔板 8完成封膜。 因此，在分注后、封盖前，并不移动 96孔板 8 , 不存在由于 96孔板 8未封盖而导致移动过程中液体的交叉污染。
实施例 2:
本实施例 2与实施例一的区别之处在于， 本实施例 2提供了另一种 封盖装置， 该封盖装置包括用于拾取预先切好的密封膜的拾取件， 移动 机构驱动拾取件将所拾取的密封膜整体覆盖并压紧在 96孔板上。
具体地，拾取件可选用一个尺寸与 96孔板匹配的平板， 而平板上设 置若干吸盘， 用以吸附密封膜。
对于拾取件所拾取的密封膜来源， 其有两种方式：
第一种方式，由外部第三方部件将根据 96孔板大小预先备好的密封 膜送至拾取件对应位置， 由拾取件直接吸附。 即在这种方式下， 移动机 构只需驱动拾取件完成竖直移动即可。 第三方部件可以为机械手、 吸盘 或静电吸附等。
第二种方式， 封盖装置在移动机构的驱动下， 自存放密封膜的区域 拾取已切好的密封膜， 然后再返回进行密封。 在这种方式下， 移动机构 可以包括水平移动机构和竖直移动机构， 水平移动机构带动拾取件水平 移动， 竖直移动机构带动拾取件竖直方向移动。
移动机构均可釆用现有常用可实现驱动其他部件移动的机构， 如气 动、 液动、 电动、 电磁驱动等驱动机构， 同时移动机构也可以包括导向 件对移动方向进行导向， 如导柱、 导槽等。
实施例 3 :
本实施例 3与实施例 2的区别之处在于， 本实施例所釆用的封盖物 为密封盖， 即与 96孔板上独立小孔所适配的盖子。
本实施例 3中， 封盖装置包括拾取件， 移动机构带动拾取件在密封 盖存放位置和 96孔板之间移动拾取并放置密封盖。
进一步地， 为了增强密封效果， 还可以包括压紧件， 在拾取件放置 好密封盖后，移动机构带动压紧件向 96孔板移动，压紧件向下压紧密封 盖， 使密封盖与对应独立小孔盖紧。
其中拾取件所拾取的密封盖可以是单独对应一个独立小孔的盖子，
也可以是根据 96孔板上小孔分布情况设置，对应多个独立小孔的一体结 构的盖子， 如用于 8连管盖， 或者 96孔盖。
拾取件可选用机械手等具有拾取功能的部件， 而压紧件可选用与 96 孔板大小相匹配的板件或其他类似部件。
实施例 4 :
本实施例 4提供一种核酸提取方法， 其包括步骤：
从样本溶液中提取得到最终核酸提取产物。
即釆用孵育、 磁分离、 洗脱等核酸提取技术提取出最终核酸提取产 物， 待以后续检测使用。
将提取的最终核酸提取产物分别注入到提取物存放容器内。
该提取物存放容器可以为 96孔板、 384孔板以及其他本领域在核酸 提取中常用作提取物存放容器的部件， 为了便于描述， 下文中均以 96 孔板为例进行说明。
其中， 本方法还包括：
在 96 孔板满足封盖条件时驱动封盖装置和安放具有多个独立小孔 的 96孔板的提取物存放装置产生相对移动， 在 96孔板不离开提取物存 放装置的情况下用封盖物密封住 96孔板。
其中，封盖装置和 96孔板相对移动包括封盖装置移动，提取物存放 装置（其上放置的 96孔板相对提取物存放装置静止）不动， 或者提取物 存放装置（其上放置的 96孔板相对提取物存放装置静止）移动， 封盖装 置不动； 再或者封盖装置和提取物存放装置（其上放置的 96孔板相对提 取物存放装置静止） 都移动。
因此， 在分注后、 封盖前， 并不手动移动 96孔板， 避免手动移动过 程中导致液体的交叉污染。
进一步地， 封盖时可使提取物存放装置保持不动， 由封盖装置相对 提取物存放装置移动进行封盖，这样在不移动 96孔板位置的情况下， 直 接由封盖装置进行封盖，可进一步避免移动 96孔板过程（即包括手动移 动， 也包括机械移动） 中造成的液体交叉污染。
其中封盖物可釆用密封膜或密封盖， 例如， 可釆用如实施例一至三 所示的封盖装置，将 96孔板上的独立小孔——密封住，待密封后再移动 96孔板。
以上应用了具体个例对本发明进行阐述， 只是用于帮助理解本发明 并不用以限制本发明。对于本领域的一般技术人员，依据本发明的思想，
可以对上述具体实施方式进行变化。
Claims
1. 一种核酸提取设备， 包括：
提取装置， 用于从样本溶液中提取核酸提取产物；
分注装置， 用于将核酸提取产物分注至提取物存放容器内； 提取物存放装置， 所述提取物存放装置用于安放具有多个独立小孔 的提取物存放容器， 所述独立小孔内分别存放最终核酸提取产物；
其特征在于还包括：
封盖装置， 用于将封盖物覆盖在提取物存放容器上；
移动机构， 用于在提取物存放容器满足封盖条件时驱动封盖装置和 提取物存放装置产生相对移动， 以使封盖装置将封盖物覆盖在提取物存 放容器上。
2. 如权利要求 1所述的核酸提取设备， 其特征在于， 封盖时， 所述 提取物存放装置保持不动， 由所述移动机构驱动封盖装置相对提取物存 放装置移动进行封盖。
3. 如权利要求 2所述的核酸提取设备， 其特征在于， 所述封盖装置 包括料辊和裁切机构， 所述料辊用于放置密封膜卷料并进行放卷， 所述 裁切机构将封盖于提取物存放容器上的密封膜从膜卷料上裁切掉。
4. 如权利要求 3所述的核酸提取设备，其特征在于， 所述封盖装置 包括还包括下压件， 所述下压件相对提取物存放容器移动， 将覆盖在提 取物存放容器上的密封膜压紧在提取物存放容器上。
5. 如权利要求 4所述的核酸提取设备，其特征在于， 所述下压件为 压辊， 所述封盖装置还包括夹辊， 所述密封膜从压辊与夹辊之间穿过。
6. 如权利要求 1所述的核酸提取设备，其特征在于， 所述封盖装置 包括用于拾取预先备好的封盖物的拾取件， 所述移动机构驱动拾取件拾 取封盖物并将所拾取的封盖物压紧封盖在提取物存放容器上。
7. 如权利要求 1-6任一项所述的核酸提取设备，其特征在于， 所述 移动机构包括水平移动机构和竖直移动机构， 所述水平移动机构带动封 盖装置水平移动， 所述竖直移动机构带动封盖装置竖直移动。
8. 如权利要求 7所述的核酸提取设备，其特征在于， 所述封盖装置 还包括加热机构， 用于对封盖物进行加热实现热封。
9. 一种核酸提取方法， 其包括步骤：
从样本溶液中提取得到最终核酸提取产物；
将提取的最终核酸提取产物分别注入到提取物存放容器的独立小孔 内；
其特征在于， 还包括：
在提取物存放容器满足封盖条件时驱动封盖装置和用于安放提取物 存放容器的提取物存放装置产生相对移动， 在提取物存放容器不离开提 取物存放装置的情况下用封盖物密封住提取物存放容器。
10. 如权利要求 9所述的方法， 其特征在于， 封盖时， 所述提取物 存放装置保持不动，由所述封盖装置相对提取物存放装置移动进行封盖。
11. 如权利要求 10所述的方法，其特征在于， 所述封盖物为密封膜 或密封盖。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2014/080129 WO2015192330A1 (zh) | 2014-06-17 | 2014-06-17 | 核酸提取设备及方法 |
| CN201480074430.6A CN105940105A (zh) | 2014-06-17 | 2014-06-17 | 核酸提取设备及方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2014/080129 WO2015192330A1 (zh) | 2014-06-17 | 2014-06-17 | 核酸提取设备及方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2015192330A1 true WO2015192330A1 (zh) | 2015-12-23 |
Family
ID=54934685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2014/080129 WO2015192330A1 (zh) | 2014-06-17 | 2014-06-17 | 核酸提取设备及方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105940105A (zh) |
| WO (1) | WO2015192330A1 (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3787791B1 (en) * | 2018-05-02 | 2021-12-15 | Geneproof A.S. | Sealer of gas-tight sealing of wells in pcr plates by this sealer |
| CN117126727A (zh) * | 2023-09-18 | 2023-11-28 | 合肥达徽基因科技有限公司 | 一种全自动提取扩增杂交一体化工作站 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070264655A1 (en) * | 2006-05-09 | 2007-11-15 | Canon Kabushiki Kaisha | Nucleic acid automatic examining device |
| US20090117004A1 (en) * | 2007-11-06 | 2009-05-07 | Abbott Laboratories | System for automatically loading immunoassay analyzer |
| CN102131913A (zh) * | 2008-08-26 | 2011-07-20 | 和光纯药工业株式会社 | 用于自动化生物样品制备的一次性装置 |
| CN202830011U (zh) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | 自动化核酸提取平台 |
| CN103282782A (zh) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | 各种生物试样分析用全自动实时定量扩增装置 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201207415Y (zh) * | 2008-05-14 | 2009-03-11 | 比亚迪股份有限公司 | 一种自动封膜机 |
| CN201695047U (zh) * | 2009-04-16 | 2011-01-05 | 上海科华生物工程股份有限公司 | 一体化血液核酸筛查平台 |
| CN102375053A (zh) * | 2010-08-20 | 2012-03-14 | 梁伟民 | 一种新型的实验室留样保存方法 |
| CN102660458B (zh) * | 2012-05-11 | 2013-11-06 | 苏州天隆生物科技有限公司 | 防止交叉污染的核酸提取装置 |
| CN103114035B (zh) * | 2013-03-14 | 2014-04-02 | 东南大学 | 一种全封闭提取、转移和贮存核酸的装置 |
| CN103818574B (zh) * | 2013-08-27 | 2016-08-10 | 新疆蓝山屯河型材有限公司 | 型材自动封膜装置和包装装置 |
| CN103482104A (zh) * | 2013-09-06 | 2014-01-01 | 华南理工大学 | 糖果盒自动封膜机 |
| CN103571740B (zh) * | 2013-10-15 | 2017-11-24 | 周国华 | 集核酸提取、扩增与检测一体化的装置 |
| CN103789198B (zh) * | 2014-02-27 | 2015-09-16 | 苏州天隆生物科技有限公司 | 全自动核酸提取仪 |
| CN203807462U (zh) * | 2014-05-16 | 2014-09-03 | 杭州奥盛仪器有限公司 | 一种用于纯化生物提取物的实验盒 |
| CN206556968U (zh) * | 2016-11-23 | 2017-10-13 | 杭州杰毅麦特医疗器械有限公司 | 一种核酸检测前处理自动化装置 |
| CN206428263U (zh) * | 2016-12-14 | 2017-08-22 | 杭州杰毅麦特医疗器械有限公司 | 核酸检测前处理自动化处理装置 |
| CN206900731U (zh) * | 2017-04-28 | 2018-01-19 | 广州市绮易美化妆品有限公司 | 一种自动封膜机 |
| CN107435083A (zh) * | 2017-09-04 | 2017-12-05 | 上海市第十人民医院 | 一种可靠的临床hbv dna样本定量检测方法 |
| CN107603859B (zh) * | 2017-11-08 | 2023-11-28 | 西安天隆科技有限公司 | 全自动一体化核酸提取、扩增及检测系统 |
-
2014
- 2014-06-17 WO PCT/CN2014/080129 patent/WO2015192330A1/zh active Application Filing
- 2014-06-17 CN CN201480074430.6A patent/CN105940105A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070264655A1 (en) * | 2006-05-09 | 2007-11-15 | Canon Kabushiki Kaisha | Nucleic acid automatic examining device |
| US20090117004A1 (en) * | 2007-11-06 | 2009-05-07 | Abbott Laboratories | System for automatically loading immunoassay analyzer |
| CN102131913A (zh) * | 2008-08-26 | 2011-07-20 | 和光纯药工业株式会社 | 用于自动化生物样品制备的一次性装置 |
| CN103282782A (zh) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | 各种生物试样分析用全自动实时定量扩增装置 |
| CN202830011U (zh) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | 自动化核酸提取平台 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3787791B1 (en) * | 2018-05-02 | 2021-12-15 | Geneproof A.S. | Sealer of gas-tight sealing of wells in pcr plates by this sealer |
| CN117126727A (zh) * | 2023-09-18 | 2023-11-28 | 合肥达徽基因科技有限公司 | 一种全自动提取扩增杂交一体化工作站 |
| CN117126727B (zh) * | 2023-09-18 | 2024-04-19 | 合肥达徽基因科技有限公司 | 一种全自动提取扩增杂交一体化工作站 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105940105A (zh) | 2016-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20190094251A1 (en) | Method of handling a laboratory sample container, laboratory apparatus and laboratory automation system | |
| CN101855558B (zh) | 试样处理器 | |
| EP4234182A3 (en) | Automated analyzer piercing stoppers for aspiration | |
| US20150233956A1 (en) | Transport device, sample distribution system, and laboratory automation system | |
| JP2017078717A5 (zh) | ||
| WO2013002216A1 (ja) | 栓体開閉機構およびそれを備えた自動分析システム | |
| US10605818B2 (en) | Automated analyzer | |
| JP2014503831A5 (zh) | ||
| JP2007064848A5 (zh) | ||
| EP2492692A1 (en) | Heat insulator | |
| WO2015192330A1 (zh) | 核酸提取设备及方法 | |
| US6080253A (en) | Method and apparatus for sealing containers | |
| US10670620B2 (en) | Pipetting device and pipetting device positioning system | |
| CN103285945B (zh) | 具有间隔件条带的封闭件 | |
| JP2020536258A (ja) | サンプル容器にキャップ形成するアプリケータ又はアプリケータシステム | |
| JP6916213B2 (ja) | 調製済み平板培地製品 | |
| EP3787791B1 (en) | Sealer of gas-tight sealing of wells in pcr plates by this sealer | |
| JP5378081B2 (ja) | 反応容器，試薬供給機構、及びそれらを用いる分析装置 | |
| US10646876B2 (en) | Biochip storage wells | |
| JP5452749B2 (ja) | 試薬供給機構及びこれを用いる分析装置 | |
| GB2570061B (en) | Adhesive film for de-capping tubes and multiwell plates | |
| KR101501201B1 (ko) | 라벨 부착장치에 사용되는 공기 주입장치 | |
| JP4912051B2 (ja) | マイクロプレート用シーラー | |
| JPH09113518A (ja) | 分注装置 | |
| JP2024054752A (ja) | ラベル貼付装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14895090 Country of ref document: EP Kind code of ref document: A1 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 14895090 Country of ref document: EP Kind code of ref document: A1 |
|
| 32PN | Ep: public notification in the ep bulletin as address of the adressee cannot be established |
Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 27/06/2017) |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 14895090 Country of ref document: EP Kind code of ref document: A1 |