WO2015192328A1 - 提取物分离装置及其工作方法 - Google Patents

提取物分离装置及其工作方法 Download PDF

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Publication number
WO2015192328A1
WO2015192328A1 PCT/CN2014/080126 CN2014080126W WO2015192328A1 WO 2015192328 A1 WO2015192328 A1 WO 2015192328A1 CN 2014080126 W CN2014080126 W CN 2014080126W WO 2015192328 A1 WO2015192328 A1 WO 2015192328A1
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WIPO (PCT)
Prior art keywords
liquid
adsorption
separation
reaction
disc
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Ceased
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PCT/CN2014/080126
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English (en)
French (fr)
Inventor
王海
王光亮
翁彦雯
黄修涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Shenzhen Mindray Bio Medical Electronics Co Ltd filed Critical Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority to CN201910679522.1A priority Critical patent/CN110488029B/zh
Priority to PCT/CN2014/080126 priority patent/WO2015192328A1/zh
Priority to CN201480074414.7A priority patent/CN105940305B/zh
Publication of WO2015192328A1 publication Critical patent/WO2015192328A1/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations

Definitions

  • the present application relates to the field of purification or concentration technology, and in particular to an extract separation device and a method of its operation.
  • the extract separation method has a wide range of applications in the field of purifying or concentrating biological substances.
  • the magnetic separation technique is usually used to extract the components of interest from the solution.
  • the basic principle is to use a small particle surface with a magnetic material to pass a certain treatment. It can adsorb the required substances, then discard the waste liquid after adsorption and enrichment by magnets, and clean the enriched magnetic beads to further remove impurities and other unwanted substances, and the biological material of interest can be adsorbed on the magnetic beads. Retaining, finally, the biological material enriched in the magnetic beads is released into the desired solution system under certain conditions.
  • Figure 1 shows a basic magnetic separation process. The steps listed are:
  • Step 1 The beads are combined.
  • the magnetic bead solution is added to the solution to be purified, and the component of interest which needs to be purified or concentrated is adsorbed onto the surface of the magnetic bead.
  • the strips are the components of interest, the blocks are impurities, and the spheres are magnetic beads.
  • Step 2 Magnetic beads adsorption and cleaning separation.
  • the magnetic beads are gathered together by an external magnetic field, thereby facilitating cleaning and removing impurities;
  • Step 3 Elution release, the cleaned magnetic beads are re-added to the desired solution to release the interesting components from the magnetic beads, or directly for subsequent testing.
  • the magnetic separation device is mainly used to complete step 2, and generally includes a liquid injection mechanism for injecting a cleaning liquid into a reaction container containing a solution for completing magnetic bead bonding, a liquid absorption mechanism for drawing waste liquid from the reaction container, and magnetic properties.
  • the mechanism and the moving mechanism are for moving the reaction container between the liquid injection mechanism and the liquid suction mechanism.
  • the reaction vessel can be removed after it has been cleaned and separated.
  • the commonly used magnetic separation device comprises a plurality of liquid absorption positions and a plurality of liquid injection positions, a magnetic field is set at the liquid absorption position, and there is no magnetic field at the liquid injection position, and each liquid absorption position is placed in a reaction container containing the reaction solution.
  • the plurality of liquid suction mechanisms respectively perform parallel liquid suction operations on the plurality of reaction containers, and after the liquid absorption is completed, the reaction containers are moved to the corresponding liquid injection positions, and then the plurality of liquid injection mechanisms respectively perform parallel operation on the plurality of reaction containers. The injection operation. Summary of the invention
  • an extract separation device comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying a reaction container along an annular space, the separating disc Having at least one liquid absorption position and at least one liquid injection position along the rotation path thereof;
  • the adsorption mechanism, the adsorption mechanism is a ring mechanism, and the adsorption mechanism is provided with one or more adsorbable extract binding carriers along the ring shape a component, and at least one vacancy position on the adsorption mechanism that does not adsorb the '1'component;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • the moving mechanism for driving the separation disc and the suction mechanism can be independently rotated and stopped, and the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup movement without the adsorption force.
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container along the annular interval, and the separating disc is distributed along the rotation path thereof Having at least one pipetting position and at least one liquid filling position;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorption components capable of adsorbing the extract binding carrier along the ring shape, and at least one of the adsorption mechanisms is not adsorbed. Vacancy position of the part;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • the movement mechanism drives the vacancy of the adsorption mechanism to follow the reaction cup of the reaction vessel Bit synchronization moves.
  • a method for magnetic separation using a magnetic separation device comprising an annular separation disk for carrying a reaction vessel and an annular adsorption mechanism
  • the separation disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container along the annular space, and the separation disc is distributed along the rotation path thereof with at least one liquid absorption position and at least one liquid injection position, the adsorption
  • the mechanism is provided with one or more adsorbing members capable of adsorbing the extract binding carrier along the ring shape, and at least one vacancy position without the adsorptive member is disposed on the adsorption mechanism; the method comprises: the separating disk drives the reaction container Rotating and stopping for operation, the vacancy position of the moving mechanism driving the adsorption mechanism moves with a position that does not need to provide an adsorption force to the extract binding carrier, and the opposite direction is stopped during the stop of the separation disk to drive the reaction container.
  • an extract separating apparatus comprising:
  • the separation disc is a rotatable ring mechanism, and the separation disc is spaced along the ring Having a plurality of reaction cups arranged in at least one row for carrying the reaction vessel, the separation disc having at least one liquid absorption position and at least one liquid injection position distributed along the rotation path thereof;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorption components capable of adsorbing the extract binding carrier along the ring shape, and at least one of the adsorption mechanisms is not adsorbed. Vacancy position of the part;
  • the moving mechanism for driving the separation disc and the suction mechanism can be independently rotated and stopped.
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container and a flow tube for carrying the waste liquid a flow tube position, the flow tube position being adjacent to the corresponding reaction cup position, the separation disc being distributed along the rotation path thereof with at least one liquid absorption position and at least one liquid injection position;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorptive members that can adsorb the extract-binding carrier along the ring shape, and the position distribution of the adsorptive member is at least compared with the suction on the separation disk.
  • the liquid position corresponds to the position, and at least one vacancy position without the adsorption component is disposed on the adsorption mechanism, the position distribution of the vacancy position corresponds to the liquid injection position; and the liquid suction mechanism for performing the liquid absorption operation at the liquid absorption position;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container and a flow tube for carrying the waste liquid a flow tube position, the flow tube position being adjacent to a corresponding reaction cup position, the separation disk being distributed along the rotation path thereof with at least one liquid absorption position, at least one liquid injection position and at least one mixed hook position, the note The liquid position and the mixed hook position are adjacent;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorptive members that can adsorb the extract-binding carrier along the ring shape, and the position distribution of the adsorptive member is at least compared with the suction on the separation disk.
  • the liquid position corresponds to the position, and at least one vacancy position without the adsorption component is disposed on the adsorption mechanism, and the position distribution of the vacancy position corresponds to at least one of the liquid injection position and the mixing position;
  • a pipetting mechanism for performing a pipetting operation at the pipetting position
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • Figure 1 is a basic magnetic separation process
  • FIG. 2 is a schematic structural view of an extract separating device in the first embodiment of the present application
  • FIG. 3 is a flow chart of extract separation in a working cycle in the first embodiment of the present application
  • FIG. 4 is a schematic structural view of a flow pipe in the second embodiment of the present application
  • FIG. 5 is a schematic view showing the separation of the extract in one working cycle in the second embodiment of the present application; and FIG. 6 is a schematic structural view of the extract separating device in the third embodiment of the present application.
  • the embodiment of the present application is intended to provide an extract separating device for performing a flow operation of a single reaction vessel as a processing unit, wherein at least one column of reaction cups for carrying a reaction vessel is distributed on the extract separating device, along the The rotation path is distributed with a plurality of liquid absorption positions and at least one liquid injection position.
  • a predetermined operation corresponding to the operation position is performed to the reaction container stopped at the liquid absorption position or the liquid injection position.
  • different operations have different requirements for the adsorption capacity of the extract-binding carrier. Some operations (or time periods) require adsorption, while others do not require adsorption. Have. Therefore, during the rotation or stop of the reaction vessel, the vacancy position at which the moving mechanism drives the adsorption mechanism without the adsorption force moves without providing an adsorption force position to the extract-binding carrier.
  • the extract separating apparatus 10 includes an adsorption mechanism 1 1 , a separation disc 12 , a liquid suction mechanism 13 , a liquid injection mechanism 14 , and a moving mechanism 15 .
  • the separating disc 12 is a ring mechanism, and the ring mechanism can be cyclically rotated, and the rotating path can be circular, elliptical, various polygonal or irregular closed shapes, and the separating disc is provided with a plurality of ring-shaped intervals for carrying the reaction.
  • the reaction cup position 121 of the container 1 22 and the plurality of reaction cup positions 1 21 are arranged in at least one column.
  • the separation disc 12 is also distributed along its rotational path with at least one liquid-absorbent position and at least one liquid-filling position.
  • the so-called liquid suction position is a position at which the reaction container 122 which is aspirating operation is stopped when the separation disk is stopped for operation, and the liquid suction mechanism 13 performs a liquid suction operation on the reaction container stopped at the liquid absorption position.
  • the so-called liquid filling position is a position at which the reaction container 122 in which the liquid filling operation is stopped when the separation disk is stopped for operation, and the liquid filling mechanism 14 performs a liquid filling operation on the reaction container stopped at the liquid filling position.
  • the liquid absorption position and the liquid injection position may each have one or more. In a specific embodiment, at least part of the liquid absorption position and the liquid injection position are alternately set, and the interval between the liquid absorption position and the liquid injection position is thousands of reaction cup positions.
  • the liquid filling position is set after a plurality of reaction cup positions 121 are separated from each other, and the liquid filling position is set after a plurality of reaction cup positions 121.
  • the number of pipetting positions is greater than the number of liquid filling positions, so that in the direction of rotation of the separation disk 12, the liquid suction position and the liquid filling position are alternately set first, and then the remaining liquid suction positions are set. In order to absorb the waste liquid more thoroughly after washing.
  • the amount of the aspiration position and the position of the infusion can be set according to the specific needs of the extract.
  • a plurality of reaction cup positions 121 are arranged in a row.
  • a plurality of reaction cup positions 121 may be arranged in two or three columns as needed, and each column may have a respective suction.
  • the liquid position and the injection position can also share the aspiration position and/or the injection position.
  • the separating disc 12 is further provided with a plurality of rings for carrying the load.
  • the flow tube position 123 of the flow tube of the waste liquid is adjacent to the corresponding reaction cup position of the flow tube position 123.
  • the flow tube is used to hold the waste liquid aspirating mechanism 1 3 The waste liquid sucked from the reaction container corresponding to the flow tube.
  • the pipetting mechanism 13 is for performing a pipetting operation at the pipetting position.
  • the pipetting mechanism 13 is located above the pipetting position, and the pipetting operation is performed by the up and down movement; in other embodiments, the pipetting The mechanism 13 may not be located above the liquid suction position but in the vicinity of the liquid suction position, the liquid suction head is moved to the upper position of the liquid suction position by rotation, and then the liquid suction operation is performed by the up and down movement.
  • the number of the liquid suction mechanisms 13 is the same as the number of liquid suction positions, and each of the liquid suction mechanisms 13 has its corresponding liquid suction position.
  • the function of the aspiration operation is to take the waste liquid from the reaction vessel, leaving the carrier-bound extract and the binding carrier.
  • the waste liquid may be the waste liquid generated after the addition of the reagent, or may be the waste liquid generated after the addition of the cleaning liquid. .
  • the liquid injection mechanism 14 is configured to perform a liquid injection operation at the liquid injection position.
  • the liquid injection mechanism 14 is located above the liquid injection position, and the liquid injection operation is performed by the up and down movement; in other embodiments, the liquid injection mechanism 14 Instead of being located above the liquid injection position, in the vicinity of the liquid injection position, the liquid injection head is moved to the upper position of the liquid injection position by rotation, and then the liquid injection operation is performed by the up and down movement.
  • the number of the liquid filling mechanisms 13 is the same as the number of the liquid filling positions, and each of the liquid filling mechanisms 14 has its corresponding liquid filling position.
  • the function of the liquid injection operation is to inject a cleaning liquid into the reaction vessel to wash the impurities adsorbed on the reaction vessel wall or the binding carrier into the solution.
  • the adsorption mechanism 11 is also a ring mechanism, and the adsorption mechanism 11 is provided with a plurality of adsorption members 111 that can adsorb the extract-binding carrier along the ring shape, and at least one of the adsorption mechanisms 11 is provided.
  • Vacancy bit 112 of the adsorbent component corresponds to the reaction cup position 121 on the separation disc 12, and the vacancy position 112 vacates at least one of the reaction cup positions without an adsorbing member.
  • the extract-binding carrier is a magnetic carrier, such as a magnetic bead
  • the adsorptive member 111 is a magnetic member such as a magnet.
  • the separation disc 12 and the adsorption mechanism 11 are annular mechanisms in which the inner and outer phases are nested.
  • the adsorption mechanism 11 may be on the outer side of the separation disc 12 or on the inner side of the separation disc 12, and has magnetic components.
  • the position corresponds to a magnetic field on the reaction cup position on the separation disc 12, and if there is a magnetic carrier adsorbed with the extract in the reaction vessel on the reaction cup position, the magnetic carrier is subjected to the magnetic force of the magnetic member, moving and adhering to the reaction
  • the container is adjacent to one side of the magnetic member; the position of the vacancy 112 having no magnetic member on the adsorption mechanism 11 corresponds to the absence of a magnetic field on the reaction cup position on the separation tray 12, and the reaction container on the reaction cup has an adsorbed extract even
  • the magnetic carrier is also not attracted by the magnetic force of the magnetic member to the side of the reaction container adjacent to the adsorption mechanism 11.
  • the adsorption mechanism 11 is located at the bottom of the separation disc 12, and when the position having the magnetic member corresponds to the reaction cup position on the separation disc 12, the magnetic carrier is subjected to the magnetic force of the magnetic member, moving and adhering to the reaction container Near the bottom of the magnetic part.
  • the vacancy 112 on the adsorption mechanism 11 may be provided only one, and the adsorption member 111 may have only one annular adsorption member; when the separation disk 12 rotates
  • the separation disk 12 rotates
  • the vacancy 112 on the adsorption mechanism 11 can also be A plurality of correspondingly arranged, and preferably the number of spaced cups between adjacent vacancies 112 is equal to the number of spaced cups between the injection locations. In order to allow the plurality of vacancies 112 to simultaneously follow a plurality of reaction cup positions that do not require adsorption.
  • the moving mechanism 15 is used to drive the separation disc 12 and the adsorption mechanism can be independently rotated and stopped.
  • the moving mechanism 15 drives the vacancy 112 of the adsorption mechanism 11 to follow the reaction vessel.
  • the reaction cup moves.
  • different operations have different requirements on the adsorption capacity of the extract-binding carrier, and sometimes an adsorption force is required, but sometimes no adsorption force is required, so the moving mechanism 15 drives the vacancy 112 of the adsorption mechanism 11 to follow the adsorption-free force.
  • the reaction cup position where the reaction vessel is located moves synchronously, so that the extract-binding carrier in the reaction vessel is not subjected to the adsorption force during this period, and the extract-binding carrier is suspended in the solution, which is advantageous for combining more extracts and cleaning.
  • the extract binds impurities on the carrier.
  • the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup position of the reaction vessel to move synchronously. , thereby making the mention in the reaction vessel
  • the material-binding carrier is not subjected to adsorption force during the mixing stage after the addition of the cleaning solution.
  • the moving mechanism 15 Before the moving mechanism 15 drives the vacancy 1 1 2 of the adsorption mechanism 11 to follow the reaction cup of the reaction vessel to start synchronous movement, it is possible that the vacancy 1 12 of the adsorption mechanism 1 1 reacts with the unwanted adsorption force on the separation disc.
  • the moving mechanism 15 first drives the suction mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to the position of the reaction container where the adsorption force is not required on the separation disc.
  • the mobile mechanism control process is as follows:
  • the moving mechanism drives the separation disc to stop for the liquid filling operation
  • the moving mechanism drives the adsorption mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to the position corresponding to the liquid injection position, and during the injection from the reaction container to the mixing hook,
  • the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup position of the reaction vessel to move synchronously.
  • the moving mechanism drives the adsorption mechanism to rotate relative to the separation disc, and transports the adsorption component to a position corresponding to the reaction cup position of the reaction container in which the end of the mixing operation is performed, and synchronizes with the reaction cup position. mobile.
  • the mixing operation is performed at the position of the mixing hook, and the position of the mixing hook is located on the rotation path of the separation disc, and the mixing position may be independent of the position of aspiration and liquid injection, or may not be located on the rotation path of the separation disc.
  • at least one of the liquid suction positions is used as a mixed hook position, and the liquid suction mechanism performs a mixed hook operation after the liquid injection by a continuous suction operation.
  • the separation method of the extract includes a sequential washing step and a separation step.
  • the cleaning steps include:
  • Step 30 a first pipetting step, the separating disk drives the reaction container to rotate, and when the separating disk stops for performing the liquid suction operation, the liquid suction mechanism performs a liquid suction operation on the reaction container stopped in the reaction cup position of the liquid suction position;
  • the adsorptive component of the adsorption mechanism corresponds to the reaction cup position in which the reaction vessel to be aspirated, provides an adsorption force for the extract-binding carrier in the reaction vessel, and the moving mechanism drives the adsorption mechanism and the separation The disc rotates and stops synchronously.
  • Step 31 The liquid filling step, when the separation tray drives the reaction container to stop for the liquid injection operation, the liquid injection mechanism performs a liquid injection operation on the reaction container stopped at the liquid injection position.
  • the moving mechanism drives the suction mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to a position corresponding to the liquid injection position, and the liquid injection mechanism performs a liquid injection operation on the reaction container stopped at the liquid injection position.
  • Step 32 The step of mixing the hook, the moving mechanism drives the separation disc and the adsorption mechanism to rotate synchronously.
  • the separation tray drives the reaction container to stop for the mixed hook operation
  • the reaction container of the reaction cup position stopped at the mixed hook position is performed.
  • the moving mechanism drives the adsorption mechanism The rotation relative to the separation disc is caused to rotate the adsorption 'ti part to a position corresponding to the reaction cup position of the reaction vessel in which the mixing is completed.
  • Step 33 the separating step comprises a second pipetting step, the separating disk drives the reaction vessel to rotate, and the adsorption component of the adsorption mechanism corresponds to the reaction cup position of the reaction vessel to be aspirated, and provides the extract binding carrier in the reaction vessel.
  • the adsorption force, and the adsorption mechanism rotates synchronously with the separation disc.
  • the liquid suction mechanism performs a liquid suction operation for the separation purpose of the reaction container in the reaction cup position stopped at the liquid absorption position.
  • the adsorption mechanism does not rotate relative to the separation disc, and it is not necessary to consider whether the vacancy position is rotated to a position corresponding to the liquid injection position, and the adsorption mechanism is driven to be separated from the separation in the mixing step.
  • the rotation of the disc rotates the vacancy position to a position corresponding to the position of the mixed hook.
  • the moving mechanism drives the suction mechanism to perform the rotation relative to the separation disc again, so that the adsorption member is rotated to the reaction container with the completion of the hook.
  • the location corresponding to the reaction cup position Example 2:
  • the liquid suction mechanism In the process of separating the extract by the above-mentioned extract separating device, the liquid suction mechanism needs to operate different reaction containers. To avoid the common liquid suction head (also called the T ip head), the liquid suction mechanism adopts a disposable liquid suction head. This requires the liquid suction mechanism to constantly replace the new liquid suction head, resulting in waste of the liquid suction head.
  • the flow tube is designed as a structure capable of carrying a disposable liquid suction head, as shown in FIG. 4, the flow tube 40 includes at least two chambers.
  • a chamber 41 is provided for the disposable liquid suction head 42 of the reaction vessel corresponding to the flow tube, and the other chamber 43 is for holding the waste liquid.
  • the solution for aspirating the disposable liquid suction head during the magnetic separation of the extract is:
  • a separation disc 12 is used to carry a reaction vessel 122 that requires magnetic separation, and a flow tube 124 for use therewith.
  • the flow tube 124 functions to carry the liquid absorption Tip and store the waste liquid;
  • the liquid suction mechanism 13 and the liquid injection mechanism 14 disposed above the operation track of the reaction container, wherein the liquid absorption mechanism is arranged in total, and the liquid injection mechanism is arranged in a total of one.
  • the liquid suction mechanism needs to be loaded with T ip before the reaction cup can be sucked.
  • a liquid magnetic field ring is disposed on the outer side of the magnetic separation disk and is rotatable concentrically with the magnetic separation disk. The magnetic field ring is fixedly mounted with a magnet for adsorbing the magnetic beads in the reaction cup.
  • the magnetic separation discs are arranged in a total of 36 positions for carrying the reaction cup and the flow tube, respectively, wherein the reaction cup and the waste tube are separated into the magnetic separation disc.
  • the reaction cup and the flow tube were taken from the magnetic separation plate by the instrument.
  • the position is placed in the magnetic separation disk one after the other, and then as the magnetic separation disk rotates forward, in the process, the corresponding outer magnetic field ring position of the reaction cup has a magnetic field, so that the magnetic bead cup is adsorbed to the side of the reaction cup;
  • the liquid suction mechanism moves downward to load the Tip in the flow tube, and then lifts up;
  • the liquid suction mechanism descends to suck the liquid in the reaction cup and then lifts up; then the magnetic separation disc continues to advance to a position, so that the flow tube is located on the magnetic separation disc At position 9, then the aspiration mechanism moves downward to drain the waste and unload Ti p.
  • the magnetic separation disk since the position of the waste liquid in the flow pipe is inconsistent with the position of the unloading Ti p, the magnetic separation disk needs to be moved again to the position where the Tip is unloaded after the waste liquid is discharged, and of course, the design of the flow tube can be made The position is kept the same, and the step of moving the magnetic separation disc is not required. So far, the first-stage aspiration movement of the magnetic separation is completed.
  • the magnetic separation disk and the magnetic field ring of the outer ring thereof are kept in synchronization or stopped, so that the reaction cup is always in the magnetic field adsorption state.
  • the magnetic separation disc continues to advance to three positions, so that the reaction cup enters the 11th position on the magnetic separation disc, that is, below the liquid injection mechanism, and the magnetic field ring occurs during the advancement of the magnetic separation disc.
  • the relative motion makes the part of the magnetic field ring without the magnetic field corresponding to the reaction cup.
  • the liquid injection mechanism injects the cleaning liquid into the reaction cup without the magnetic field adsorption, and then the magnetic separation plate retreats back to the three positions so that the flow tube returns to the first In the first-stage aspiration position, the aspirating mechanism is lowered to load the Tip, and then the magnetic separation disc continues to retreat to a position, so that the reaction cup is located below the first-stage aspiration position, and the liquid suction mechanism descends to suck and discharge the cleaning liquid and the magnetic beads in the reaction cup.
  • the magnetic separation disc is further advanced to a position, so that the flow tube is located under the liquid suction mechanism to unload the Tip, and the first stage of the magnetic separation and the mixing action are completed, and the magnetic separation disk and the magnetic field ring are completed in the process.
  • the synchronous movement and stopping are maintained at all times, so the reaction cup is always in a state of no magnetic field adsorption, and the magnetic beads are resuspended in the cleaning liquid.
  • the magnetic field ring is re-rotated relative to the magnetic separation disc so that the reaction cup is in a magnetic field, and the magnetic field adsorption of the next step is continued.
  • the second-order magnetic separation aspiration and injection mixing operation is repeated with the above process, and then the third stage only performs the liquid absorption operation, and does not perform the liquid injection operation, sucking the liquid in the reaction cup, the fourth order is the same as the third
  • the steps are repeated, and only the liquid is absorbed by the liquid to absorb the residual liquid at the bottom of the cup. At this point, the entire magnetic separation operation is completed. Then the reaction cup and the flow tube continue to move back to position 36 with the magnetic separation disc, and the reaction cup and the flow tube are taken away by the instrument for subsequent operations.
  • the above process is designed for a specific extraction characteristic. According to the difference in the degree of cleanliness required for extraction and the difference in residual liquid, the number of times of aspirating and injecting can be increased or decreased, even in the end, the need for subsequent injection testing is increased.
  • the other reaction liquid components are all possible deformation schemes.
  • the separation disc can also be provided with only one liquid absorption position and one liquid injection position along its rotation path.
  • one working cycle is: first, the reaction container is transported to the liquid absorption position to perform the suction liquid waste. Operate, then transfer the reaction vessel to the fill level The injection liquid is injected into the reaction container, and finally the reaction container is transported to the liquid absorption position to perform the operation of sucking the waste liquid.
  • Example 3
  • the separation disc is a rotatable ring mechanism for driving the separation disc to rotate and stop.
  • the adsorption mechanism is a fixed non-rotating annular structure, and the adsorption mechanism is provided with a plurality of adsorption components capable of adsorbing the extract-binding carrier along the ring shape, and the position distribution of the adsorption component corresponds at least to the liquid absorption position on the separation disk.
  • the adsorption mechanism is provided with at least one vacancy position without an adsorption component, and the position distribution of the vacancy position corresponds to at least one of the liquid injection position and the mixing position, and the position distribution of the vacancy position and the injection position and the mixing position When they are all corresponding, the injection position and the mixing position are adjacent.
  • the separation process of the extract will be described below by taking magnetic separation as an example.
  • a rotatable magnetic separation disk 60 is provided with a plurality of reaction cups on the upper surface for carrying the reaction cup 61 requiring magnetic separation, and a magnetic field structure is arranged at a fixed position of the outer ring of the magnetic separation disk.
  • the magnetic member 62 is configured to adsorb magnetic beads in the reaction cup, and a liquid injection mechanism 63 and a liquid suction mechanism 64 that are movable up and down are disposed above the magnetic separation disk.
  • the magnetic field structure also includes a void location 65 having no magnetic components, and the void location 65 corresponds to the liquid injection location.
  • the reaction cup is placed in the magnetic separation disk, and the magnetic beads in the reaction cup are adsorbed to the side of the reaction cup by the magnetic field outside the magnetic separation disk, and then the reaction cup rotates forward with the magnetic separation disk, moving magnetic
  • the separation disc drives the reaction cup to continue moving forward, the reaction cup reaches below the liquid injection mechanism, and the liquid injection mechanism moves downward to inject the cleaning liquid into the reaction cup and mixes it. There is no magnetic field distribution outside the reaction cup below the liquid injection mechanism.
  • the magnetic beads are resuspended in the cleaning solution. After that, the magnetic separation disc drives the reaction cup to move forward to the next liquid suction mechanism.

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Abstract

一种提取物分离装置及其分离方法，提取物分离装置包括吸附机构（11）、分离盘（12）、吸液机构（13）、注液机构（14）和移动机构（15）、分离盘为可旋转的环形机构，并沿环形间隔设有多个反应杯位（121），分离盘沿其旋转途径分布有多个吸液位置和至少一个注液位置，吸附机构也为环形机构，吸附机构沿环形设有多个吸附性部件（111），且所述吸附机构上至少设置一个没有吸附性部件的空缺位（112），移动机构驱动分离盘和吸附机构可独立地旋转和停止，使空缺位随不需要吸附力位置而移动。由于分离盘和吸附机构可独立地旋转和停止，使得吸附机构可根据需要提供吸附力或不提供吸附力。

Description

提取物分离装置及其工作方法 技术领域
本申请涉及提纯或浓缩技术领域， 具体涉及提取物分离装置及其工 作方法。
背景技术
提取物分离方法在提纯或浓缩生物物质领域有着广泛的应用， 通常 釆用磁分离技术从溶液中对感兴趣组分进行提取， 其基本原理是利用具 有磁性材料的小微粒表面通过一定的处理使其可以吸附所需要物质， 然 后通过磁铁吸附富集后抛弃废液， 并对富集的磁珠进行清洗， 进一步清 除杂质及其他不需要的物质， 而感兴趣生物物质因吸附在磁珠上得以保 留， 最后在一定条件下将富集在磁珠上的生物物质释放到所需要的溶液 体系中。 如图 1所示为一个基本的磁分离流程， 所列步骤分别为：
步骤 1 : 磁珠结合。 向需要提纯的溶液中加入磁珠溶液， 将需要提 纯或浓缩的感兴趣组分吸附到磁珠表面。 图中条状为感兴趣组分， 块状 为杂质， 球状为磁珠。
步骤 2: 磁珠吸附及清洗分离。 通过外加磁场使得磁珠聚集在一起， 进而便于清洗去除杂质；
步骤 3、 洗脱释放， 完成清洗的磁珠重新加入所需要的溶液将感兴 趣组分从磁珠上释放下来 , 或直接进行后续的测试。
磁分离装置主要用于完成步骤 2 , 通常包括用于向盛装有完成磁珠 结合的溶液的反应容器中注入清洗液的注液机构、 用于从反应容器中吸 取废液的吸液机构、 磁性机构和移动机构， 移动机构用于将反应容器在 注液机构和吸液机构之间移动。 反应容器完成清洗分离后即可被移走。
目前常用的磁分离装置包括多个吸液位置和多个注液位置， 在吸液 位置设置磁场， 而在注液位置没有磁场， 每个吸液位置放入一个装有反 应溶液的反应容器， 多个吸液机构分别对多个反应容器执行并行的吸液 操作， 待吸液完成后各反应容器被移动到对应的注液位置， 然后多个注 液机构再分别对多个反应容器执行并行的注液操作。 发明内容
依据本申请的第一方面， 在一种实施方式中提供一种提取物分离装 置，包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置； 吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止， 移动 机构驱动吸附机构的空缺位跟随不需要吸附力的反应杯位移动。
依据本申请的第二方面， 在另一种实施方式中提供一种提取物分离 装置， 包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止， 至少 在分离盘上的反应容器加入清洗液处于混勾阶段时， 移动机构驱动吸附 机构的空缺位跟随该反应容器的反应杯位同步移动。
依据本申请的第三方面， 在一种实施方式中提供一种釆用磁分离装 置进行磁分离的方法， 所述磁分离装置包括用于承载反应容器的环形分 离盘和环形吸附机构， 所述分离盘沿环形间隔设有多个排布成至少一列 的用于承载反应容器的反应杯位， 所述分离盘沿其旋转途径分布有至少 一个吸液位置和至少一个注液位置， 所述吸附机构沿环形设有一个或多 个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至少设置 一个没有吸附性部件的空缺位； 所述方法包括： 所述分离盘带动反应容 器旋转并为做操作而停止， 移动机构驱动吸附机构的空缺位随不需要对 提取物结合性载体提供吸附力的位置而移动， 在分离盘带动反应容器为 做操作的停止期间， 对向停止在吸液位置或注液位置的反应容器执行预 定操作。
依据本申请的第四方面， 在一种实施方式中提供一种提取物分离装 置， 包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止。
依据本申请的第五方面， 在一种实施方式中提供一种提取物分离装 置， 包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位和用于承载盛 装废液的流转管的流转管位， 所述流转管位与其对应的反应杯位相邻， 所述分离盘沿其旋转途径分布有至少一个吸液位置和至少一个注液位 置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 所述吸附性部件的位 置分布至少与分离盘上的吸液位置相对应， 且所述吸附机构上至少设置 一个没有吸附性部件的空缺位， 空缺位的位置分布与注液位置相对应； 用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘旋转和停止。
依据本申请的第六方面， 在一种实施方式中提供一种提取物分离装 置， 包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位和用于承载盛 装废液的流转管的流转管位， 所述流转管位与其对应的反应杯位相邻， 所述分离盘沿其旋转途径分布有至少一个吸液位置、 至少一个注液位置 和至少一个混勾位置， 所述注液位置和混勾位置相邻；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 所述吸附性部件的位 置分布至少与分离盘上的吸液位置相对应， 且所述吸附机构上至少设置 一个没有吸附性部件的空缺位， 空缺位的位置分布至少与注液位置和混 匀位置中的一者相对应；
用于在吸液位置执行吸液操作的吸液机构； 用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘旋转和停止。 附图说明
图 1是一个基本的磁分离流程；
图 2是本申请实施例一中提取物分离装置的结构示意图；
图 3是本申请实施例一中一个工作周期中提取物分离流程图； 图 4是本申请实施例二中流转管的结构示意图；
图 5是本申请实施例二中一个工作周期中提取物分离示意图； 图 6是本申请实施例三中提取物分离装置的结构示意图。
具体实施方式
本申请实施例意在提供了一种以单个反应容器为处理单元进行流转 操作的提取物分离装置， 在提取物分离装置上分布有至少一列构成环形 的用于承载反应容器的反应杯位， 沿其旋转途径分布有多个吸液位置和 至少一个注液位置， 反应容器旋转并为做操作而停止时， 向停止在吸液 位置或注液位置的反应容器执行与该操作位置相应的预定操作， 但不同 的操作对提取物结合性载体吸附力的要求不同， 有些操作 （或者是时间 段） 需要有吸附力， 而有些不需要有吸附力。 有。 因此在反应容器旋转 或停止过程中， 移动机构驱动吸附机构上没有吸附力的空缺位随不需要 对提取物结合性载体提供吸附力位置而移动。 实施例 1 :
如图 2所示， 提取物分离装置 10包括吸附机构 1 1、 分离盘 12、 吸 液机构 1 3、 注液机构 14和移动机构 1 5。
分离盘 1 2为环形机构， 该环形机构可循环转动， 其旋转途径可以是 圓形、 椭圓形、 各种多边形或不规则的封闭形状， 分离盘沿环形间隔设 有多个用于承载反应容器 1 22的反应杯位 121 , 多个反应杯位 1 21排布 成至少一列。分离盘 1 2沿其旋转途径还分布有至少一个吸液位置和至少 一个注液位置。 所称吸液位置为当分离盘为做操作而停止时， 为做吸液 操作的反应容器 122所停止的位置，吸液机构 1 3对停止在吸液位置的反 应容器执行吸液操作。 所称注液位置为当分离盘为做操作而停止时， 为 做注液操作的反应容器 122所停止的位置，注液机构 14对停止在注液位 置的反应容器执行注液操作。吸液位置和注液位置可以各有一个或多个， 在一种具体实施例中， 至少部分吸液位置和注液位置交替设置， 吸液位 置和注液位置之间间隔若千反应杯位 1 21 , 即沿分离盘 1 2的旋转方向， 一个吸液位置间隔若干反应杯位 121后设置注液位置， 该注液位置间隔 若干反应杯位 121后设置吸液位置。 有些实施例中吸液位置的数量多于 注液位置的数量， 因此沿分离盘 12的旋转方向，先是交替设置的吸液位 置和注液位置， 然后设置剩余的吸液位置。 以便在清洗之后更彻底地吸 取废液。 吸液位置和注液位置的数量可根据提取物具体的需求设置。 本 实施例中， 多个反应杯位 121排布成一列， 在其它实施例中， 可根据需 要将多个反应杯位 121排布成两列或三列， 各列之间可以有各自的吸液 位置和注液位置， 也可以共用吸液位置和 /或注液位置。
为防止吸液结构将吸取的废液转运到较远的地方而提高交叉污染的 几率和增加转运的时间，在较佳的实施例中，分离盘 12沿环形还设有多 个用于承载盛装废液的流转管的流转管位 123 , 流转管位 123与其对应 的反应杯位相邻。流转管用于盛装废液吸液机构 1 3从流转管对应的反应 容器中吸取的废液。
吸液机构 1 3用于在吸液位置执行吸液操作 ,一种具体实施例中吸液 机构 1 3位于吸液位置上方，通过上下运动来执行吸液操作； 在其它实施 例中，吸液机构 1 3可以不位于吸液位置的上方，而位于吸液位置的附近， 通过旋转将吸液头移动到吸液位置的上方， 然后再通过上下运动来执行 吸液操作。 本实施例中， 吸液机构 13的数量与吸液位置的数量相同， 每 个吸液机构 1 3有其对应的吸液位置。
吸液操作的作用是从反应容器中吸取废液， 留下被载体结合的提取 物和结合性载体， 废液可以是加入试剂后产生的废液， 也可以是加入清 洗液之后产生的废液。
注液机构 14用于在注液位置执行注液操作，一种具体实施例中注液 机构 14位于注液位置上方，通过上下运动来执行注液操作； 在其它实施 例中，注液机构 14可以不位于注液位置的上方，而位于注液位置的附近， 通过旋转将注液头移动到注液位置的上方， 然后再通过上下运动来执行 注液操作。 本实施例中， 为注液机构 13的数量与注液位置的数量相同， 每个注液机构 14有其对应的注液位置。
注液操作的作用是向反应容器中注入清洗液， 以便将吸附在反应容 器壁上或结合性载体的杂质清洗到溶液中。
吸附机构 11也为环形机构， 吸附机构 11沿环形设有多个可吸附提 取物结合性载体的吸附性部件 111 ,且吸附机构 11上至少设置一个没有 吸附性部件的空缺位 112。吸附性部件 111与分离盘 12上的反应杯位 121 对应， 空缺位 112即空出至少一个反应杯位没有吸附性部件。 当提取物 结合性载体为磁性载体时， 例如磁珠， 吸附性部件 111为磁性部件， 例 如磁铁。 一种具体实例中， 分离盘 12和吸附机构 11为内外相嵌套的圓 环机构， 具体实例中， 吸附机构 11可以在分离盘 12的外侧， 也可以在 分离盘 12的内侧， 具有磁性部件的位置对应于分离盘 12上的反应杯位 上具有磁场， 该反应杯位上的反应容器中如果具有吸附有提取物的磁性 载体， 则磁性载体受磁性部件的磁力作用， 移动并紧贴反应容器靠近磁 性部件的一侧；吸附机构 11上没有磁性部件的空缺位 112的位置对应于 分离盘 12上的反应杯位上没有磁场，该反应杯位上的反应容器中即使具 有吸附有提取物的磁性载体， 也不会受磁性部件的磁力作用而向反应容 器靠近吸附机构 11的一侧聚集。 在另一具体实例中， 吸附机构 11位于 分离盘 12的底部， 当具有磁性部件的位置对应于分离盘 12上的反应杯 位时， 磁性载体受磁性部件的磁力作用， 移动并紧贴反应容器靠近磁性 部件的底部。 具体实例中， 当分离盘 12旋转途径只有一个注液位置时， 吸附机构 11上的空缺位 112可以只设置一个，吸附性部件 111也可以只 有一个环状的吸附性部件； 当分离盘 12旋转途径有多个注液位置（例如 两个或三个）和多个吸液位置时， 意味着分离盘 12上可同时对多个反应 容器进行预定操作， 吸附机构 11上的空缺位 112也可以对应设置多个， 且优选相邻空缺位 112之间的间隔杯位数等于注液位置之间的间隔杯位 数。以便使多个空缺位 112同时跟随多个不需要吸附力的反应杯位移动。
移动机构 15用于驱动分离盘 12和吸附机构可独立地旋转和停止， 当分离盘 12上的反应容器不需要提供吸附力时， 移动机构 15驱动吸附 机构 11的空缺位 112跟随该反应容器的反应杯位移动。具体实例中， 不 同的操作对提取物结合性载体吸附力的要求不同， 有时需要有吸附力， 但有时不需要吸附力， 因此移动机构 15驱动吸附机构 11的空缺位 112 跟随不需要吸附力的反应容器所在的反应杯位同步移动， 从而使得反应 容器中的提取物结合性载体在此期间不受吸附力作用， 提取物结合性载 体悬浮在溶液中， 有利于结合更多的提取物和清洗提取物结合性载体上 的杂质。 一种具体实例中， 在反应容器旋转或停止过程中， 至少在分离 盘上的反应容器加入清洗液处于混勾阶段时， 移动机构驱动吸附机构的 空缺位跟随该反应容器的反应杯位同步移动， 从而使得反应容器中的提 取物结合性载体在加入清洗液后的混勾阶段不受吸附力作用。 在移动机构 1 5驱动吸附机构 11的空缺位 1 1 2跟随该反应容器的反 应杯位开始同步移动前，可能吸附机构 1 1的空缺位 1 12与分离盘上的该 不需要吸附力的反应容器所在位置不同时，此种情况下移动机构 1 5先驱 动吸附机构做相对于分离盘的转动， 使空缺位旋转到分离盘上的该不需 要吸附力的反应容器所在位置。
一种具体实例中， 移动机构控制过程如下：
移动机构驱动分离盘为做注液操作而停止时， 移动机构驱动吸附机 构做相对于分离盘转动，使空缺位旋转到与注液位置对应的位置，且从反 应容器注液到混勾期间， 移动机构驱动吸附机构的空缺位跟随该反应容 器的反应杯位同步移动。 混勾完成后， 移动机构再次驱动吸附机构做相 对于分离盘的转动， 将吸附'ί部件转运到与结束混勾操作的反应容器所 在的反应杯位对应的位置， 并跟随该反应杯位同步移动。
混匀操作在混勾位置执行， 混勾位置位于分离盘的旋转途径上， 混 匀位置可以是独立于吸液和注液的位置， 也可以不位于分离盘的旋转途 径上。 一种较佳的实施例中， 将吸液位置中的至少一个作为混勾位置， 吸液机构通过连续的吸注操作执行注液后的混勾操作。
对于从将反应容器放入分离盘到完成分离的每个工作周期， 提取物 的分离方法包括顺序进行的清洗步骤和分离步骤。 如图 3所示， 清洗步 骤包括：
步骤 30 , 第一吸液步骤， 分离盘带动反应容器旋转， 当分离盘为做 吸液操作而停止时， 吸液机构对停止在吸液位置的反应杯位中的反应容 器执行吸液操作； 在第一吸液步骤中， 吸附机构的吸附性部件与将进行 吸液的反应容器所在的反应杯位对应， 为反应容器中提取物结合性载体 提供吸附力， 且移动机构驱动吸附机构和分离盘同步旋转和停止。
步骤 31 ,注液步骤， 当分离盘带动反应容器为作注液操作而停止时， 注液机构对停止在注液位置的反应容器执行注液操作。 同时移动机构驱 动吸附机构做相对于分离盘的转动，使空缺位旋转到与注液位置对应的 位置， 注液机构对停止在注液位置的反应容器执行注液操作。
步骤 32 , 混勾步骤， 移动机构驱动分离盘和吸附机构同步旋转， 当 分离盘带动反应容器为作混勾操作而停止时， 对停止在混勾位置的反应 杯位中的反应容器执行混勾操作， 混勾完成后， 移动机构驱动吸附机构 做相对于分离盘的转动，使吸附 ' ti部件旋转到与完成混勾的反应容器所 在反应杯位对应的位置。
步骤 33 , 分离步骤包括第二吸液步骤， 分离盘带动反应容器旋转， 吸附机构的吸附性部件与将进行吸液的反应容器所在的反应杯位对应， 为反应容器中提取物结合性载体提供吸附力， 且吸附机构随分离盘同步 旋转， 当分离盘为做吸液操作而停止时， 吸液机构对停止在吸液位置的 反应杯位中的反应容器执行为分离目的的吸液操作。
在其它实施例中，在注液步骤中吸附机构不做相对于分离盘的转动， 不需要考虑空缺位是否旋转到与注液位置对应的位置， 在混勾步骤中驱 动吸附机构做相对于分离盘的转动， 使空缺位旋转到与混勾位置对应的 位置 ,混勾操作完成后，移动机构驱动吸附机构再次做相对于分离盘的转 动 ,使吸附性部件旋转到与完成混勾的反应容器所在反应杯位对应的位 置。 实施例 2:
在一些新的领域， 例如核酸分析中， 由于该领域内对测试间的交叉 污染要求高， 因此整个核酸提取流程中需要严格避免测试间共用器件。 在釆用上述提取物分离装置分离提取物过程中， 吸液机构需要对不同的 反应容器进行操作， 为避免共用吸液头（也称 T i p头）， 吸液机构采用一 次性吸液头， 这要求吸液机构不断更换新的吸液头， 造成了吸液头的浪 费。 为解决一次性吸液头频繁更换的问题， 在本申请一种具体实例中， 将流转管设计为可承载一次性吸液头的结构， 如图 4 所示， 流转管 40 包括至少两个腔，一个腔 41用于放置与该流转管对应的反应容器的一次 性吸液头 42 , 另外的腔 43用于盛放废液。 提取物磁分离过程中釆用一 次性吸液头吸液的方案为：
请再次参考图 2 , —个分离盘 12 用于承载需要磁分离的反应容器 122 , 以及与之配套使用的流转管 124 , 流转管 124的作用是用来承载吸 液 Ti p以及存放废液；布置在反应容器运行轨迹上方的吸液机构 1 3以及 注液机构 14 , 其中吸液机构共布置 4个， 注液机构共布置 1个， 吸液机 构需要装载 T i p后才可以吸取反应杯中的液体； 在磁分离盘的外侧布置 一个可与磁分离盘同心旋转的磁场环， 磁场环的固定位置安装有磁铁用 于吸附反应杯中的磁珠。
在一个具体实施例里面， 如图 5 所示， 磁分离盘上共均排布有 36 个位置， 分别用于承载反应杯和流转管， 其中反应杯和废液管间隔进入 磁分离盘。 在工作开始时， 反应杯和流转管被仪器从磁分离盘上第 36 号位置一前一后放入磁分离盘， 然后随着磁分离盘旋转递进， 在此过程 中反应杯对应的外侧磁场环位置具有磁场， 使得磁珠杯吸附到反应杯一 侧； 当流转管随着磁分离盘旋转到磁分离盘上第 9号位置即第一阶的吸 液机构下方时， 吸液机构向下运动装载流转管中的 Tip , 然后抬起； 其 后磁分离盘向后退一个位置， 使得反应杯刚好进入磁分离盘上第 9号位 置， 吸液机构下降吸取反应杯中的液体后抬起； 然后磁分离盘继续前进 一个位置， 使得流转管位于磁分离盘上第 9号位置， 然后吸液机构向下 运动排出废液并卸载 Ti p。 在此过程中， 由于流转管内排废液位置与卸 载 Ti p位置并不一致，因此需要排完废液后磁分离盘再次运动到卸载 Tip 的位置， 当然也可以通过流转管的设计， 使得两个位置保持一致， 则不 需要磁分离盘运动的步骤。 至此磁分离第一阶吸液动作完成， 在上述过 程中， 磁分离盘和其外圈的磁场环一致保持同步运动或停止， 因此反应 杯一直处于磁场吸附状态下。 完成第一阶吸液动作后， 磁分离盘继续前 进 3个位置 , 使得反应杯进入到磁分离盘上第 11号位置即注液机构下 方， 在磁分离盘前进过程中， 磁场环与之发生相对运动使得磁场环上没 有磁场的部分对应反应杯， 此时注液机构在没有磁场吸附作用下向反应 杯内注入清洗液， 其后磁分离盘回退 3个位置使得流转管重新回到第一 阶吸液位置， 吸液机构下降装载 Tip , 然后磁分离盘继续后退一个位置， 使得反应杯位于第一阶吸液位置下方， 吸液机构下降对反应杯内的清洗 液和磁珠进行吸排混勾后抬起， 然后磁分离盘继续前进一个位置， 使得 流转管位于吸液机构下方卸载 Tip , 至此磁分离第一阶注液及混勾动作 完成， 在此过程中磁分离盘与磁场环一直保持同步运动和停止， 因此反 应杯一直处于无磁场吸附的状态下， 便于磁珠重新悬浮于清洗液中。 完 成混勾后磁场环重新与磁分离盘相对转动使得反应杯处于有磁场的状态 下， 继续下一阶的磁场吸附。 第二阶的磁分离吸液及注液混匀动作与上 述过程重复， 然后第三阶则只进行吸液动作， 不进行注液动作， 吸干反 应杯内的液体， 第四阶同第三阶动作重复， 不注液只吸液进一步吸干杯 底的残留液体。 至此， 整个磁分离动作完成。 然后反应杯和流转管随着 磁分离盘继续运动回到 36号位置，由仪器取走反应杯和流转管进行后续 的操作。
当然上述过程是针对一个特定的提取特性而设计， 根据提取所需要 的洁净程度的不同以及对残留液体的不同， 可以增加或者减少吸液和注 液的次数， 甚至在最后增加注入后续测试所需要的其他反应液成分， 都 是可能的变形方案。
上述实施例中， 分离盘沿其旋转途径也可以只设置一个吸液位置和 一个注液位置， 对于一个反应容器， 其一个工作周期为： 先将反应容器 转运到吸液位置执行吸取废液的操作， 然后再将反应容器转运到注液位 置， 向反应容器内注入清洗液， 最后再将反应容器转运到吸液位置执行 吸取废液的操作。 实施例 3 :
与上述实施例不同的是分离盘为可旋转的环形机构， 移动机构用于 驱动分离盘旋转和停止。 吸附机构为固定不旋转的环形结构， 吸附机构 沿环形设有多个可吸附提取物结合性载体的吸附性部件， 所述吸附性部 件的位置分布至少与分离盘上的吸液位置相对应， 且所述吸附机构上至 少设置一个没有吸附性部件的空缺位， 空缺位的位置分布与注液位置和 混匀位置中的至少一个对应， 当空缺位的位置分布与注液位置和混匀位 置都相对应时， 注液位置和混匀位置相邻。 下面以磁分离为例对提取物 的分离过程进行说明。
如图 6所示， 一个可旋转的磁分离盘 60 , 上面均勾分布多个反应杯 位， 用于承载需要磁分离的反应杯 61 , 在磁分离盘的外圈固定位置布置 有磁场结构， 例如磁性部件 62 , 用于吸附反应杯内的磁珠， 磁分离盘上 方分别布置有可上下运动的注液机构 63和吸液机构 64。 磁场结构还包 括一个没有磁性部件的空缺位 65 , 空缺位 65与注液位置对应。 磁分离 过程开始时， 反应杯被放入磁分离盘， 反应杯内的磁珠被磁分离盘外部 的磁场吸附到反应杯的一侧， 随后反应杯随着磁分离盘向前旋转， 运动 磁分离盘带动反应杯继续向前运动， 反应杯到达注液机构下方， 注液机 构向下运动向反应杯中注入清洗液并进行混匀， 位于注液机构下方的反 应杯外侧没有磁场分布 , 此时磁珠被重新悬浮到清洗液中。 其后磁分离 盘带动反应杯继续向前运动到下一个吸液机构下方， 在此过程中磁分离 盘外圈的磁铁再次将磁珠吸附到反应杯的一侧， 吸液机构重复上一阶的 动作， 其后动作重复直至完成最后一次清洗及吸液动作。 反应杯完成磁 分离后即可被取出。 以上应用了具体个例对本申请进行阐述， 只是用于帮助理解本申请 并不用以限制本申请。对于本领域的一般技术人员，依据本申请的思想， 可以对上述具体实施方式进行变化。

Claims

权 利 要 求
1. 一种提取物分离装置，其特征在于包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止， 移动 机构驱动吸附机构的空缺位跟随不需要吸附力的反应杯位移动。
2. 一种提取物分离装置，其特征在于包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止， 至少 在分离盘上的反应容器加入清洗液处于混勾阶段时， 移动机构驱动吸附 机构的空缺位跟随该反应容器的反应杯位同步移动。
3. 如权利要求 1或 2所述的提取物分离装置， 其特征在于， 所述 移动机构驱动分离盘为做注液操作而停止时， 移动机构驱动吸附机构 的空缺位旋转到与注液位置对应的位置，且从反应容器注液到混匀期 间， 移动机构驱动吸附机构的空缺位跟随该反应容器的反应杯位同步移 动，混勾完成后， 移动机构再次驱动吸附机构的吸附性部件转运到与结 束混勾操作的反应容器所在的反应杯位对应的位置， 并跟随该反应杯位 同步移动。
4. 如权利要求 3所述的提取物分离装置， 其特征在于， 所述吸液 机构还通过连续的吸注操作执行注液后的混勾操作。
5. 如权利要求 1或 2所述的提取物分离装置， 其特征在于， 所述 分离盘和吸附机构为内外相嵌套的圓环机构， 或吸附机构位于分离盘的 底部。
6. 如权利要求 1或 2所述的提取物分离装置， 其特征在于， 所述 吸附性部件为可吸附磁性载体的磁性部件。
7. 如权利要求 1 -6 中任一项所述的提取物分离装置， 其特征在 于，所述吸液位置的数量多于注液位置，所述分离盘沿环形顺序分布有： 交替设置的吸液位置和注液位置， 以及吸液位置。
8. 如权利要求 7所述的提取物分离装置， 其特征在于， 至少有两 个注液位置， 所述吸附机构上的空缺位的数量等于注液位置的数量， 所 述吸附性部件的位置分布与分离盘上的反应杯位相对应， 所述空缺位之 间的间隔杯位数与注液位置之间的间隔杯位数相同。
9. 如权利要求 1 -8 中任一项所述的提取物分离装置， 其特征在 于， 所述分离盘沿环形还设有多个用于承载盛装废液的流转管的流转管 位 , 所述流转管位与其对应的反应杯位相邻。
1 0. 一种釆用磁分离装置进行磁分离的方法， 所述磁分离装置包 括用于承载反应容器的环形分离盘和环形吸附机构， 所述分离盘沿环形 间隔设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分 离盘沿其旋转途径分布有至少一个吸液位置和至少一个注液位置， 所述 吸附机构沿环形设有一个或多个可吸附提取物结合性载体的吸附 ' 1·生部 件， 且所述吸附机构上至少设置一个没有吸附性部件的空缺位； 其特征 在于所述方法包括： 所述分离盘带动反应容器旋转并为做操作而停止， 移动机构驱动吸附机构的空缺位随不需要对提取物结合性载体提供吸 附力的位置而移动， 在分离盘带动反应容器为做操作的停止期间， 对向 停止在吸液位置或注液位置的反应容器执行预定操作。
1 1. 如权利要求 1 0所述的方法， 其特征在于， 对于从将反应容器 放入分离盘到完成分离的每个工作周期， 所述方法包括顺序进行的清洗 步骤和分离步骤， 所述清洗步骤顺序包括：
第一吸液步骤， 分离盘带动反应容器旋转， 当分离盘为做吸液操作 而停止时， 吸液机构对停止在吸液位置的反应杯位中的反应容器执行吸 液操作； 在第一吸液步骤中， 吸附机构的吸附性部件与将进行吸液的反 应容器所在的反应杯位对应， 为反应容器中提取物结合性载体提供吸附 力， 且移动机构驱动吸附机构和分离盘同步旋转和停止；
注液步骤， 当分离盘带动反应容器为作注液操作而停止时， 移动机 构驱动吸附机构做相对于分离盘的转动，使空缺位旋转到与注液位置对 应的位置， 注液机构对停止在注液位置的反应容器执行注液操作；
所述分离步骤包括：
第二次吸液步骤， 分离盘带动反应容器旋转， 吸附机构的吸附性部 件与将进行吸液的反应容器所在的反应杯位对应， 为反应容器中提取物 结合性载体提供吸附力， 且吸附机构随分离盘同步旋转， 当分离盘为做 吸液操作而停止时， 吸液机构对停止在吸液位置的反应容器执行为分离 目的的吸液操作。
12. 如权利要求 11 所述的方法， 其特征在于， 所述清洗步骤在注 液步骤之后还包括：
混匀步骤， 移动机构驱动分离盘和吸附机构同步旋转， 当分离盘带 动反应容器为作混勾操作而停止时， 对停止在混勾位置的反应杯位中的 反应容器执行混勾操作， 混勾完成后， 移动机构驱动吸附机构做相对于 分离盘的转动，使吸附性部件旋转到与完成混勾的反应容器所在反应杯 位对应的位置。
13. 如权利要求 10所述的方法， 其特征在于， 对于从将反应容器 放入分离盘到完成分离的每个工作周期， 所述方法包括顺序进行的清洗 步骤和分离步骤， 所述清洗步骤顺序包括：
第一吸液步骤， 分离盘带动反应容器旋转， 当分离盘为做吸液操作 而停止时， 吸液机构对停止在吸液位置的反应杯位中的反应容器执行吸 液操作； 在第一吸液步骤中， 吸附机构的吸附性部件与将进行吸液的反 应容器所在的反应杯位对应， 为反应容器中提取物结合性载体提供吸附 力， 且移动机构驱动吸附机构和分离盘同步旋转和停止；
注液步骤， 当分离盘带动反应容器为作注液操作而停止时， 注液机 构对停止在注液位置的反应容器执行注液操作；
混匀步骤， 当分离盘带动反应容器为作混勾操作而停止时， 对停止 在混勾位置的反应容器执行混勾操作， 混勾操作过程中， 移动机构驱动 吸附机构做相对于分离盘的转动，使空缺位旋转到与混勾位置对应的位 置，混勾操作完成后，移动机构驱动吸附机构再次做相对于分离盘的转动 使吸附性部件旋转到与完成混勾的反应容器所在反应杯位对应的位置； 所述分离步骤包括：
第二次吸液步骤， 分离盘带动反应容器旋转， 吸附机构的吸附性部 件与将进行吸液的反应容器所在的反应杯位对应， 为反应容器中提取物 结合性载体提供吸附力， 且吸附机构随分离盘同步旋转， 当分离盘为做 吸液操作而停止时， 吸液机构对停止在吸液位置的反应杯位中的反应容 器执行为分离目的的吸液操作。
14. 如权利要求 1 2或 1 3所述的方法， 其特征在于， 所述混勾位置 位于吸液位置， 所述吸液机构还通过连续的吸注操作执行注液后的混匀 操作。
15. 如权利要求 14所述的方法， 其特征在于， 所述分离盘沿环形 还设有多个用于承载流转管的流转管位， 所述流转管位与其对应的反应 杯位相邻； 第一吸液步骤和第二吸液步骤分别包括：
将承载有吸液头的、 与将执行吸液操作的反应容器对应的流转管转 运到吸液位置 , 吸液机构从停止在吸液位置的流转管上装载吸液头； 将该流转管对应的反应容器转运到吸液位置， 装载了吸液头的吸液 机构从停止在吸液位置的反应容器中吸取废液；
将与完成吸液操作的反应容器对应的流转管转运到吸液位置， 吸液 机构向停止在吸液位置的流转管中排出废液， 然后将吸液头卸载到流转 管中。
16. 如权利要求 1 5所述的方法， 其特征在于， 所述混勾步骤包括： 将承载有吸液头的、 与将执行混勾操作的反应容器对应的流转管转 运到吸液位置 , 吸液机构从停止在吸液位置的流转管上装载吸液头； 将该流转管对应的反应容器转运到吸液位置， 装载了吸液头的吸液 机构对停止在吸液位置的反应容器执行吸排混匀操作；
将与完成混勾操作的反应容器对应的流转管转运到吸液位置， 吸液 机构将吸液头卸载到流转管中。
17. 如权利要求 1 1至 1 6中任一项所述的方法， 其特征在于， 所述 清洗步骤执行多次， 所述第二次吸液步骤执行多次。
18. 一种提取物分离装置，其特征在于包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位， 所述分离盘 沿其旋转途径分布有至少一个吸液位置和至少一个注液位置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 且所述吸附机构上至 少设置一个没有吸附 ' 1·生部件的空缺位；
移动机构， 用于驱动分离盘和吸附机构可独立地旋转和停止。
1 9. 一种提取物分离装置，其特征在于包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位和用于承载盛 装废液的流转管的流转管位， 所述流转管位与其对应的反应杯位相邻， 所述分离盘沿其旋转途径分布有至少一个吸液位置和至少一个注液位 置；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 所述吸附性部件的位 置分布至少与分离盘上的吸液位置相对应， 且所述吸附机构上至少设置 一个没有吸附性部件的空缺位， 空缺位的位置分布与注液位置相对应； 用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘旋转和停止。
20. 一种提取物分离装置，其特征在于包括：
分离盘， 所述分离盘为可旋转的环形机构， 所述分离盘沿环形间隔 设有多个排布成至少一列的用于承载反应容器的反应杯位和用于承载盛 装废液的流转管的流转管位， 所述流转管位与其对应的反应杯位相邻， 所述分离盘沿其旋转途径分布有至少一个吸液位置、 至少一个注液位置 和至少一个混勾位置， 所述注液位置和混勾位置相邻；
吸附机构， 所述吸附机构为环形机构， 所述吸附机构沿环形设有一 个或多个可吸附提取物结合性载体的吸附性部件， 所述吸附性部件的位 置分布至少与分离盘上的吸液位置相对应， 且所述吸附机构上至少设置 一个没有吸附性部件的空缺位， 空缺位的位置分布与注液位置和混匀位 置中的至少一者相对应；
用于在吸液位置执行吸液操作的吸液机构；
用于在注液位置执行注液操作的注液机构；
移动机构， 用于驱动分离盘旋转和停止。
21. 如权利要求 1 8-20中任一项所述的提取物分离装置，其特征在 于， 所述吸附性部件为可吸附磁性载体的磁性部件， 所述吸附机构为固 定不旋转结构， 所述分离盘和吸附机构构成内外相嵌套的机构， 或吸附 机构位于分离盘的底部。
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