WO2015172429A1

WO2015172429A1 - Camellia oil liquid preparation for prevention and treatment of oral diseases

Info

Publication number: WO2015172429A1
Application number: PCT/CN2014/081979
Authority: WO
Inventors: 史璟璟
Original assignee: 深圳市恒昇启泰进出口有限公司
Priority date: 2014-05-12
Filing date: 2014-07-10
Publication date: 2015-11-19
Also published as: HK1198745A1; TW201542223A; CN103989991A

Abstract

Disclosed is a camellia oil liquid preparation for the prevention and treatment of oral diseases, a major component thereof being camellia oil. The camellia oil liquid preparation achieves the purpose of prevention and treatment of oral diseases by lubrication, nourishment, moisture retention and sterilization of each tissue in the oral cavity, such as mucosa and gingiva, etc., in particular for oral ulcers and gingivitis.

Description

Description A liquid preparation of camellia oil for preventing and treating oral diseases

Technical field

The present invention relates to a natural vegetable oil liquid preparation for preventing and treating oral diseases, and more particularly to a liquid preparation containing a main component of camellia oil. Background technique

Everyone has ulcer troubles, mostly in any part of the oral mucosa, mostly in the lips, cheeks and tongue. In severe cases, it may spread to the pharyngeal mucosa, usually white ulcers, surrounded by redness, pain, especially It is more intense when encountering stimulating foods, such as: sour, salty, spicy food, which greatly affects people's daily lives. For oral ulcers, Chinese medicine treatment generally uses the method of purging fire and detoxification, clearing dampness and heat. In addition, medication can also be used. The cause of recurrent oral ulcers is still unclear. It is widely believed that there are many causes of oral ulcers. For example, busy work makes people mentally stressed, mood swings, and lack of sleep. In this case, autonomic dysfunction is likely to occur. The chance is higher. Infection with microorganisms such as bacteria, viruses, malnutrition, vitamin deficiency, digestive system dysfunction, such as bloating, diarrhea or constipation. In addition, oral ulcers are also thought to be related to factors such as heredity and hormones. Gingivitis and periodontitis are also common diseases and frequently-occurring diseases, which often cause great pain to people's lives.

People with oral ulcers, periodontal disease, gum swelling and other diseases often have an unpleasant smell. The bad breath is related to digestive dysfunction. It is caused by gastrointestinal dysfunction or indigestion, which changes the saliva composition. It is acidic and is beneficial to the breeding activities of spoilage bacteria, resulting in bad breath. This also brings a lot of inconvenience to people's daily lives.

Camellia oil (also known as wild camellia oil, tea seed oil, camellia seed oil) is taken from the seeds of Camellia oleifera. The production process of camellia oil is usually divided into: shelling, drying, smashing, oil extraction, filtration, and the whole process is physical, so it is a true pure natural green cooking oil. Tea oil does not contain erucic acid, cholesterol, aflatoxin and other additives. Tested: Unsaturated fatty acid in tea oil is more than 90%, oleic acid reaches 80-83%, linoleic acid reaches 7-13%, and is rich in protein and vitamins, B, D,

E, etc., especially the rich linolenic acid it contains is essential for the human body and cannot be synthesized. According to scientific identification, the content of oleic acid and linoleic acid of camellia oil is higher than that of olive oil. China's camellia oil has been eating history for more than 3,000 years. The Ming Dynasty medical scientist Li Shizhen mentioned the therapeutic effect of tea oil in the "Compendium of Materia Medica": "tea seed, bitter cold and fragrant, attending panting and coughing, to remove dirt." There are also many other records in other ancient books. The "The Compendium of the Compendium" records that "tea oil can treat hemorrhoids and dehumidification fever". "The diet of the farmhouse" records that "tea oil is cooked and cooked, suitable for daily use, steamed food, and light is produced. The oils are the most light, so the diseases are not taboo." The medical doctor Li Shizhen's "Compendium of Materia Medica" records that "tea oil is cold, cooling blood to stop bleeding, clearing away heat and detoxification. Indications for liver and blood loss, deworming. Benefit stomach, eyesight" , and cloud "tea seeds. Bitter and fragrant, the main asthma and cough, go to the disease."

Currently, chemically synthesized fungicides and ionic surfactants are used in products for the prevention and treatment of oral diseases. Such chemicals are highly irritating to the oral mucosa, and sometimes cause severe pain when used (such as patients with oral ulcers), and bacteria are susceptible to such chemicals. Based on this, there is a need to develop a natural extract-based liquid preparation for preventing and treating oral diseases, and liquid preparation products for preventing and treating oral diseases mainly based on camellia oil have not appeared. Summary of the invention

The object of the present invention is to provide a liquid preparation of camellia oil for preventing and treating oral diseases, which achieves prevention by lubricating, moisturizing, moisturizing and sterilizing various tissues such as mucous membranes and gums in the oral cavity. And the purpose of treating oral diseases (especially oral ulcers and gingivitis, etc.).

The object of the present invention is achieved by the following technical solutions:

The present invention provides a camellia oil liquid preparation for preventing and treating oral diseases, and the main component is camellia oil, which preferably has a specific gravity of 80-100 wt%, more preferably 80-90 wt%.

Preferably, the above camellia oil liquid preparation further comprises the following raw material extracts: white peony, forsythia, platycodon, reed rhizome, gypsum and mountain bean root.

Among them, white peony: Function: disperse wind and dehumidification, alleviate pain, relieve swelling and pus; Indications: For cold headache, eyebrow pain, nasal congestion, nose, toothache, vaginal discharge, sore throat. It is used in an amount of 0 to 4% by weight, preferably 0.5 to 2.5% by weight based on the total weight of the camellia oil liquid preparation.

Forsythia: Function: Qingrejiedu, Sanjie swelling, evacuation of wind and heat; Indications: Chinese medicine treatment of fever, wind, fever, fever, upset, sore throat, macule, erysipelas, sputum, hemorrhoids , acute nephritis, hot shower. It is used in an amount of 0 to 3 wt%, preferably 0.5 to 1.5 wt%, based on the total weight of the camellia oil liquid preparation.

Platycodon grandiflorum: Function: Xuanfei, Liyan, phlegm, drainage; Indications: For cough, chest tightness, sore throat, dumbness, lungs, pus, sore pus. It is used in an amount of from 0 to 4% by weight, preferably from 0.5 to 1.5% by weight, based on the total weight of the camellia oil liquid preparation.

Lugan: Function: Clearing heat and fluid, removing trouble, vomiting, diuretic; Indications: For fever, polydipsia, stomach heat and vomiting, lung heat cough, lungs, pus, hot dripping pain. It is used in an amount of from 0 to 4% by weight, preferably from 0.5 to 1.5% by weight, based on the total weight of the camellia oil liquid preparation.

Gypsum: Function: Clearing heat and purging fire, in addition to annoying thirst; Indications: For exogenous fever, high fever and polydipsia, lung heat and cough, stomach fire, sputum, headache, toothache. It is used in an amount of from 0 to 5% by weight, preferably from 0.5 to 3.5% by weight, based on the total weight of the camellia oil liquid preparation.

Shandou Root: Function: Qingrejiedu, swelling and pharyngeal; Indications: For fire poisoning, sore throat, gum swelling and pain. It is used in an amount of from 0 to 5% by weight, preferably from 0.5 to 3.5% by weight, based on the total weight of the camellia oil liquid preparation.

Preferably, the above-mentioned camellia oil liquid preparation may further be added to one or more of the following raw material extracts: Nepeta, Laiwuzi, sassafras, celery, alfalfa, Prunella vulgaris, lemon, olive, banana, radix isatidis, Millet (rich in vitamin B2), green leafy vegetables (rich in vitamin B2), Sophora flavescens, bandits, banana leaves, bitter gourd, bamboo leaves, almonds, mint, licorice, honey, Vitamin B2, Astragalus, Honeysuckle, Houttuynia, Fat Sea, Ginger, Tianqi, Coptis, Tea. The amount used is the balance of the amounts of the aforementioned components (camellia oil, white peony, forsythia, platycodon, reed rhizome, gypsum and mountain bean root), and the sum of the components of the camellia oil liquid preparation is 100% by weight.

The invention also provides a refining process of camellia oil, which comprises the following steps:

Alkali refining

Test the acidification of the camellia oil prepared for alkali refining, and add the corresponding proportion of alkali and salt according to the acid value, specifically: adding 0.5-2Kg of alkali (NaOH) per ton (lOOOKg) of wool 1.5 to 2.5 kg of edible salt (Nacl).

Preferably, the optimum process conditions for alkali refining are: the acid value of the hair oil is 1-10, and the acid value after alkali refining is controlled at 0-0.1.

2. Washing, degumming

After the alkali refining oil, first raise the oil temperature to 75-85 ° C, add 8-12 1% of 100 ° C hot water per ton of oil, stir, oil, soap feet, water three layers, after precipitation soapstock and oil water separation in the separating process of the simultaneous removal of phospholipids, i.e. dephosphorization (degumming), such that the amount of residual phospholipids camellia oil reaches 5 * 10_ ⁶ mol / l or less;

Preferably, the number of times of washing and degumming is 2-3 times, and each time is 1-2 hours.

3. Decolorization

The camellia oil separated after washing and degumming is heated to 100 °C-115 °C, and added to white clay (food additive) for decolorization of 4-8 1% of oil per ton of oil and 0.4-0.8 1% activated carbon to remove benzopyrene. Then cool down to 80-9 CTC and filter again. The process takes 2-4 hours.

Preferably, the optimum process conditions for decolorization are: adding white clay (food additive) and 0.5-0.6% activated carbon at 5%, and decolorizing at 100 ° C - 115 ° C for 3 hours.

4. Deodorization

The tea oil is placed under vacuum through a heat transfer tubing for at least 4 hours without adding any additives. Preferably, during this process, the smoke point is raised from 1 KTC to 240 ° C and then to below 1 KTC.

5. Freezing (freezing)

The temperature of the camellia oil is lowered to 0 ° C to -2 ° C, and the temperature is 24-48 hours to precipitate vegetable fat and wax (floc).

6. Filter

After freezing, it is filtered through a filter cloth and then filtered through a filter paper. The filtered oil is camellia oil.

Preferably, the acid value of the above-mentioned refined camellia oil is controlled to be 0.15 or less, preferably 0.1 or less.

Tested: The unsaturated fatty acid in camellia oil obtained by the above process is as high as 93%, oleic acid reaches over 82%, and linoleic acid reaches more than 13%.

The invention adopts camellia oil as a basic functional oil of a liquid preparation, which is not only nutrient-rich but also has high osmotic lubrication and moisturizing function, and has good curative effect on oral diseases such as oral ulcers and gingivitis, and is proportional to white peony, The combination of forsythia, platycodon, reed rhizome, gypsum and yam root extract is more effective for the above oral diseases. Further, since the basic functional fat of the liquid preparation of the present invention, camellia oil itself has good corrosion resistance, a small amount (0.05-0.1%) of potassium sorbate preservative can be selectively added to the preparation of the present invention.

The invention also provides a preparation process of a camellia oil liquid preparation for preventing and treating oral diseases, which specifically comprises the following steps:

1 According to the formula amount, weigh the camellia oil, white peony root extract, forsythia extract, platycodon grandiflorum extract, reed rhizome extract, gypsum extract and mountain bean root extract;

2 stirring and mixing the white peony extract, forsythia extract, platycodon grandiflorum extract, reed rhizome extract, gypsum extract and mountain bean root extract;

3 During the high-speed stirring process (1000-2000 rpm), the mixture in step 2 is slowly added to the camellia oil at 30-6 CTC and mixed uniformly;

4 Filter and sterilize.

Optionally, other natural extracts can also be added.

The invention also provides an application of camellia oil as a main raw material in preparing a medicament for preventing and treating oral diseases.

Preferably, the oral disease is oral ulcer, gingivitis or the like.

The camellia oil liquid preparation prepared by the invention for preventing and treating oral diseases is a pure natural preparation, can ensure safe and reliable oral cavity, has good lubrication and moisturizing property, can deeply moisturize oral and pharyngeal mucosa, reduce and kill The harmful bacteria in the dead mouth have a long duration of action and have a good preventive and therapeutic effect on oral diseases such as oral ulcers and gingivitis. The clinical results of the liquid preparation of the invention show that the preparation can significantly improve various indexes of diseases such as oral ulcer and gingivitis, and has no adverse reactions and side reactions, and is safe and reliable, and can fully meet the clinical requirements. detailed description

The present invention will now be further described with examples to illustrate how the invention is implemented:

[Preparation Example 1]

Preparation of camellia oil:

Alkali refining

The oil prepared for alkali refining is tested and the acid value is measured. According to the acid value, the corresponding proportion of alkali and salt are added, specifically: 0.7 kg of NaOH per ton (lOOOKg) of wool and 1.8 kg of edible salt Nacl. The optimum process conditions for alkali refining are: The acid value of the oil is 1-10, and the acid value after alkali refining is controlled at about 0.1.

2. Washing, degumming

After the alkali-refined oil, first raise the oil temperature to 8o °c, add 10%% of ioo °c hot water per ton of oil, and oil after stirring. Soap, three water, after precipitation with the soapstock and oil water separation in the separating process of the simultaneous removal of phospholipids, i.e. dephosphorization (degumming), such that the amount of residual phospholipids camellia oil reaches 5 * 10_ ⁶ mol /l or less; The above washing and degumming process is repeated twice, each time is about 1 hour.

3. Decolorization

The camellia oil separated after washing and degumming is heated to 110°C-115°C, 5wt% white clay (food additive) and 0.6wt% activated carbon are added per ton of camellia oil, and then cooled to 90°C and then filtered. The time is controlled in about 3 hours.

4. Deodorization

Through the heat transfer tubing, the camellia oil is under vacuum for at least 4 hours. This process does not add any additives and then falls below ioo°c.

5. Freezing (freezing)

The temperature of the camellia oil was lowered to 0 ° C to -2 ° C, and the temperature was kept for 32 hours to precipitate vegetable fat and wax (floc).

6. Filter

After freezing, it is passed through a thick filter cloth (110 mesh - 150 mesh) and then filtered through a thick filter paper. The filtered oil is camellia oil.

It has been tested that the acid value of the above-mentioned refined camellia oil is controlled to be below 0.01, and the unsaturated fatty acid in the obtained camellia oil is as high as 93% or more, oleic acid is above 82%, and linoleic acid is 13%.

[Embodiment 1]

Preparation of lKg liquid preparation of the invention according to the following weight percentage formula

Preparation of Camellia oil of Example 1 97 wt% _;

White peony root extract 0.5wt% _;

Forsythia extract 0.5wt% _;

Platycodon grandiflorum extract 0.5wt% _;

Reed extract 0.5wt% _;

Gypsum extract 0.5wt% _;

Soybean root extract 0.5wt%.

The preparation method comprises the following steps: 1 according to the formula amount, respectively extracting camellia oil, white peony root extract, forsythia extract, platycodon grandiflorum extract, reed rhizome extract, gypsum extract and mountain bean root extract;

3 In the process of high-speed stirring (100 rpm), slowly add the mixture in step 2 to camellia oil at 30 ° C Evenly mixed;

4 Filtration, sterilization with isotope cobalt 60 auxiliary. (Can the Camellia oil be sterilized by irradiation? Can it be processed into a powder after the Chinese medicine is processed into a powder, and then sterilized by irradiation of the powder and added to the camellia oil.)

[Embodiment 2]

Camellia oil of Preparation Example 1 was 93%;

White peony extract lwt% _;

Forsythia extract lwt% _;

Platycodon grandiflorum extract lwt% _;

Reed root extract lwt% _;

Gypsum extract 1.5wt% _;

Soybean root extract 1.5wt%.

3 in the process of high-speed stirring (100 rpm), at 50 ° C, the mixture in step 2 is slowly added to the camellia oil and mixed evenly;

4 Filtration, sterilization with isotope cobalt 60 auxiliary.

[Embodiment 3]

Preparation of Camellia oil of Example 1 90%;

White peony extract lwt%;

Forsythia extract 1.5wt%;

Platycodon grandiflorum extract 1.5wt%;

Reed extract 0.5wt%;

Gypsum extract 3.5wt%;

Soybean root extract 2wt%.

The preparation method comprises the following steps: 1 according to the formula amount, respectively, extracting camellia oil, white peony root extract, forsythia extract, platycodon grandiflorum extract, Reed extract, gypsum extract and mountain bean root extract;

3 During the high-speed stirring process (1500 rpm), the mixture in step 2 was slowly added to the camellia oil at 50 ° C to mix well;

4 Filtration, sterilization with isotope cobalt 60 auxiliary.

[Embodiment 4]

Preparation of Camellia Oil of Example 1 85 %

White peony extract 2.5wt% _;

Forsythia extract 2wt% _;

Platycodon grandiflorum extract 0.5wt% _;

Reed extract 4wt% _;

Gypsum extract 3wt% _;

Soybean root extract 3wt% _;

3 During the high-speed stirring process (1500 rpm), the mixture in step 2 was slowly added to the camellia oil at 40 ° C to mix well;

4 Filtration, sterilization with isotope cobalt 60 auxiliary.

[Embodiment 5]

Preparation of Camellia oil of Example 1 87 wt% _;

White peony extract 2.5wt% _;

Forsythia extract 1.5wt% _;

Platycodon grandiflorum extract lwt% _;

Reed extract 1.5wt% _; Gypsum extract 3.5wt%;

Soybean root extract 3wt%.

3 During the high-speed stirring process (1800 rpm), the mixture in step 2 was slowly added to the camellia oil at 40 ° C to mix well;

4 Filtration, sterilization with isotope cobalt 60 auxiliary.

[Embodiment 6]

Preparation of Camellia oil of Example 1 90%;

White peony extract lwt % _;

Perforatum extract _1.5wt%;

Platycodon grandiflorum extract 1.5wt% _;

Reed extract 0.5wt% _;

Gypsum extract 3wt% _;

Soybean root extract 2wt% _;

Peppermint extract 0.5wt% _o

The preparation method comprises the following steps: 1 according to the formula amount, respectively, extracting camellia oil, white peony root extract, forsythia extract, platycodon grandiflorum extract, reed rhizome extract, gypsum extract, mountain bean root extract and peppermint extract;

2 stirring and mixing the white peony root extract, forsythia extract, platycodon grandiflorum extract, reed rhizome extract, gypsum extract, mountain bean root extract and peppermint extract;

3 During the high-speed stirring process (1800 rpm), the mixture in step 2 was slowly added to the camellia oil at 35 ° C to mix well;

4 Filtration, sterilization with isotope cobalt 60 auxiliary. Clinical trial on oral ulcers

In order to investigate whether the liquid preparation of the camellia oil of the present invention is effective in improving oral ulcers, we conducted a clinical experiment. Experimental methods: Patients with oral ulcers were recruited as volunteers in the hospital for clinical trial research. The main methods are:

For 25 volunteers, only patients with oral ulcers on the medial mucosa of the upper and lower lips were selected for easy observation and measurement. A group of 5 people was divided into 5 groups for experimental observation, in which the first group did not use any drug (comparative example), and the other four groups used Preparation Example 1 (pure camellia oil) and the camellia prepared in Examples 1, 3 and 6 respectively. Oil liquid preparation.

Specific treatment methods: Use a cotton swab to extract camellia oil or its liquid preparation to cover the oral wound, three times a day, about 7:00 in the morning, 11:00 in the morning and 10:00 in the evening. The size of the ulcer area was observed daily (the oral ulcer is usually round, so the average change of five people is taken as the diameter of the ulcer). The results are shown in Table 1.

Table I

From the above experimental results, it is known that the use of the camellia oil and the camellia oil liquid preparation of the present invention can significantly reduce the area of the oral ulcer and significantly improve the healing rate of the oral ulcer. Clinical trial on gingivitis

In order to investigate whether the liquid preparation of the camellia oil of the present invention is effective in improving gingivitis, we have also carried out clinical experimental research methods: recruiting patients with gingivitis as a volunteer in a clinical trial study in a hospital, the main methods are: selecting 25 volunteers A group of 5 people was divided into 5 groups for experimental observation, in which the first group did not use any drug (prospective), and the other four groups were prepared using Preparation Example 1 (pure camellia oil) and Examples 1, 3 and 6 respectively. Camellia oil liquid preparation.

Specific treatment: Use a cotton swab to extract camellia oil or its liquid preparation on the gums, three times a day, about 7:00 am, 11:00 am and 10:00 pm. Based on the symptoms of the third and fifth days of each group of patients. The results are shown in Table 2. Gingivitis symptoms

Experimental sample 0 days 3 days 5 days

Comparative proportion (blank) swollen pain, swelling, pain, swelling and pain

Preparation Example 1 Swelling and pain Symptoms are slightly relieved Symptoms are alleviated Example 1 Swelling and pain Symptoms are slightly relieved Symptoms are alleviated Example 3 Swelling and pain Symptoms are alleviated Symptoms are significantly alleviated Example 6 Swelling and pain Symptoms are slightly relieved Symptoms are alleviated

Table II

From the above experimental results, it is known that the use of the camellia oil and the camellia oil liquid preparation of the present invention can significantly alleviate the inflammation of the gums and reduce the degree of gum swelling in the patient.

Claims

Claim

A camellia oil liquid preparation for preventing and treating oral diseases, characterized in that the main component is camellia oil, and the specific gravity thereof is preferably from 80 to 100% by weight, more preferably from 80 to 90% by weight.

The camellia oil liquid preparation according to claim 1, wherein the camellia oil liquid preparation further comprises the following raw material extracts: white peony, forsythia, platycodon, reed rhizome, gypsum and mountain bean root.

The camellia oil liquid preparation according to any one of claims 1 to 2, wherein the chalk is used in an amount of 0 to 4 wt%, preferably 0.5 to 2.5 wt%, based on the total weight of the camellia oil liquid preparation; The amount of warp is 0-3 wt%, preferably 0.5-1.5 wt%, based on the total weight of the camellia oil liquid preparation; the amount of the platycodon is 0-4 wt<3⁄4, preferably 0.5-1.5 wt%, based on the total weight of the camellia oil liquid preparation The amount of the reed root is 0-4wt<3⁄4, preferably 0.5-1.5wt%, based on the total weight of the camellia oil liquid preparation; the gypsum is used in an amount of 0-5 wt%, preferably 0.5-3.5 wt%, based on the camellia oil liquid The total weight of the preparation; the amount of the soybean root is 0-5 wt%, preferably 0.5-3.5 wt%, based on the total weight of the camellia oil liquid preparation.

The camellia oil liquid preparation according to claim 3, wherein the camellia oil liquid preparation is further added to one or more of the following raw material extracts: schizonepeta, raisin, medlar, celery, Pheasant, Prunella vulgaris, lemon, olive, banana, radix isatidis, millet (rich in vitamin B2), green leafy vegetables (rich in vitamin B2), Sophora flavescens, bandit, banana leaf, bitter gourd, bamboo leaves, almonds, mint, Licorice, honey, vitamin B2, astragalus, honeysuckle, houttuynia, fat sea, ginger, Tianqi, berberine, tea; preferably used in the aforementioned components (camellia oil, white peony, forsythia, platycodon, reed rhizome, gypsum and The balance of the amount of the soybean bean root, the sum of the components of the camellia oil liquid preparation is 100wt<3⁄4.

5. The refining process of camellia oil according to any one of claims 1 to 4, characterized in that it comprises the following steps:

6. Filter

7. The process for preparing a camellia oil liquid preparation according to any one of claims 1 to 4, characterized in that:

Contains the following steps:

4 Filter and sterilize.

8. The process for preparing a camellia oil liquid preparation according to claim 7, characterized in that: other natural extracts may be added during the preparation.

A use of camellia oil as a main raw material for the preparation of a medicament for preventing and treating oral diseases. Preferably, the oral cavity disease is oral ulcer, gingivitis or the like.