Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
  • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
  • C07D239/72—Quinazolines; Hydrogenated quinazolines
  • C07D239/86—Quinazolines; Hydrogenated quinazolines with hetero atoms directly attached in position 4
  • C07D239/88—Oxygen atoms
  • C07D239/91—Oxygen atoms with aryl or aralkyl radicals attached in position 2 or 3
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  • A61P25/00—Drugs for disorders of the nervous system
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  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
  • A61P25/16—Anti-Parkinson drugs
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  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/16—Otologicals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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• • A—HUMAN NECESSITIES
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  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Definitions

• the present invention relates to heterocyclic compounds based on a quinazoline scaffold which bind effectively to the mitochondrial translocator protein (TSPO), and counteract cell death processes.
• the present invention further provides pharmaceutical compositions including such compounds, and methods of using these compounds, especially for treating and preventing neurodegenerative diseases as well as brain damage due to acute events (e.g., traumatic brain injury (TBI) or its after effects), or chronic challenges (e.g., infection, toxins, or drug use), including the underlying pathological processes.
• TBI traumatic brain injury
• challenges e.g., infection, toxins, or drug use
• Neurodegeneration due to neurodegenerative brain disease and injury is a major health and economic concern.
• Current models predict at least a more than threefold rise in the total number of persons with Alzheimer's disease between 2000 and 2050.
• a well accepted concept for a basic cause for neurodegeneration is overexcitation by glutamate as well as impairments of mitochondria leading to death of nerve cells in the brain (Camins et al. Methods Find Exp Clin Pharmacol. 2008; 30:43-65; Nakamura et al. Apoptosis. 2010;15: 1354-63).
• Potential treatments have been designed and tested, for example, by inhibition of glutamate receptors. However, such approaches have not matured to effective treatments, probably because the causes for neurodegeneration are not well understood (Byrnes et al.
• glia constitutes the vast majority of brain cells, and serves to maintain and protect healthy neurons. Indeed it is now well realized that glia presents a critical factor in the progress of neurodegeneration (Rossi et al. Brain Res Bull. 2009;80:224-32). Thus, glia should be one of the factors to be included for the development of effective treatments for neurodegeneration .
• Traumatic brain injury is characterized by sudden physical damage to the brain. It is caused by many factors including warfare, terrorism, motor vehicle accidents and other traffic accidents, work related accidents, sports injuries, violent crimes, household accidents, child abuse and domestic violence, or by an object passing through the skull, for example gunshot wounds, etc.
• the physical, behavioral, and / or mental changes that may result from TBI depend on the areas of the brain that are injured. Injuries include focal and diffuse brain damage. Focal damage is confined to a small area of the brain. The focal damage is most often at the point where the head hits an object or where an object, such as a bullet, enters the brain. Diffuse damage is spread throughout the brain.
• Mitochondria are one of the cell organelles affected by secondary brain injury and in particular collapse of the mitochondrial membrane potential ( ⁇ ) appears to take a central role among the factors leading to neuronal cell death due to secondary brain injury and neurodegenerative diseases (Kluger et al. 2004; Gaetz et al. 2004). This suggests that targeting the regulation of the ⁇ , immediately after sustained brain injury, would have significant therapeutic implications for TBI. As the TSPO is a major component for these mechanisms, targeting the TSPO to treat secondary brain injury, neurodegenerative diseases, and related conditions represents a novel and potentially very effective therapeutic approach.
• the TSPO has been associated with the voltage dependent anion channel (VDAC), located in the outer mitochondrial membrane, which functions as a major channel allowing passage of small molecules and ions between the mitochondrial inter-membrane space and cytoplasm (McEnery et al. Proc Natl Acad Sci U S A. 1992;89:3170-4; Veenman et al. Anticancer Agents Med Chem. 2014;14:559-77). Opening of the VDAC can contribute to collapse of the ⁇ .
• the TSPO has also been associated the adenine nucleotide translocator (ANT), which is located in the inner mitochondrial membrane, and can form a lethal pore also contributing to collapse of the ⁇ .
• ANT adenine nucleotide translocator
• TSPO programmed cell death processes
• TSPO was found to be involved in the generation of ROS that take part in the induction of the mitochondrial apoptosis cascade, as well as other forms of programmed cell death.
• ROS are known to cause detachment of cytochrome c from cardiolipins located at the inner mitochondrial membrane.
• ROS contribute to pore opening in the outer mitochondrial membrane allowing release of cytochrome c into the cytosol.
• TSPO was first detected by its capability to bind benzodiazepines in peripheral tissues and later was detected also in glial cells in the brain, hence its previous name peripheral benzodiazepine receptor (PBR) (Papadopoulos et al. Trends Pharmacol Sci. 2006; 27:402-9; Veenman and Gavish. Pharmacol Ther. 2006;110:503-24; Veenman et al. Curr Pharm Design. 2007;13:2385-2405). It is known that this mitochondrial protein can be pharmacologically regulated by specific ligands (Veenman et al. Neurochem. 2002;80:917-27; Veenman and Gavish. Pharmacol Ther.
• PBR peripheral benzodiazepine receptor
• TSPO functions include: regulation of cell death processes, regulation of the cell cycle, regulation of gene expression, modulation of steroid production, involvement in cell migration, involvement in cell differentiation, involvement in angiogenesis, involvement in excitotoxic cell death, and involvement in inflammation and immune responses.
• the expression of TSPO is enhanced during neurodegeneration occurring, e.g., in Alzheimer, Parkinson, and Huntington disease, and also due to brain trauma (Gavish et al. Pharmacol Rev.
• TSPO Silencing the TSPO by genetic manipulation (siRNA and antisense RNA) prevents programmed cell death, including cell death induced by glutamate.
• TSPO levels are increased in U118MG cells exposed to lethal levels of glutamate.
• U118MG cells are of astrocytic origin.
• the TSPO presents a promising target to protect the brain from neuropathological processes (Veiga et al. 2007; Panickar et al. Glia. 2007;55: 1720- 7).
• TSPO Apart from its role in cell death, other elucidated functions under the control of the TSPO, such as regulation of gene expression, involvement in inflammation and immune response, involvement in cell migration, cell differentiation, involvement in angiogenesis, and its specific involvement in excitotoxic cell death, emphasize the importance of the TSPO as a target for treatment against cell death in the brain due to disease and brain trauma, including its contribution to repair and restorative mechanisms (Bode et al. Pharmacogenet Genomics. 2012;22:538-50; Veenman et al. Pharmacogenet Genomics. 2012). It has previously been found that the classical TSPO ligands PK 11195 and Ro5 4864 present moderate neuroprotective effects in vivo (see, e.g., Veenman et al.
• United States Patent No. 8,541,428 discloses phthalazine, quinazoline, and quinoxaline derivatives, pharmaceutical compositions containing the compounds, and their therapeutic use in treating and preventing brain damage resulting from traumatic brain injuries (TBI), and in treating and preventing neurodegenerative diseases.
• Compounds disclosed in said patent publication were shown to bind to the PBR (TSPO) with varying affinity and were shown to reduce cell death induced by glutamate in cell culture. Furthermore, cell protection conferred by these compounds occurred with neuronal type cells (SH SY 5Y) as well as glial cell types (Ul 18MG).
• United States Patent No. 6,765,006 discloses quinazolines and other heterocycles which are antagonists or positive modulators of AMPA receptors, and the use thereof for treating, preventing or ameliorating neuronal loss or treating or ameliorating neurodegenerative diseases.
• the present invention relates to compounds described by formula (I), as well as individual compounds described herein, which are based on a quinazoline scaffold substituted with an amide group and an attached rotatable phenyl group optionally substituted by a halogen.
• the present invention further relates to pharmaceutical compositions containing the compounds, and their therapeutic use in treating and preventing brain injuries resulting from acute or chronic events and their after effects, as well as treatment of brain damage associated with brain diseases that include degeneration of neurons and/or glial cells.
• the compounds of the invention also inhibit mechanisms of programmed cell death (i.e., they prevent cell death), and they can stimulate cell activity related to replenishing of dead brain cells.
• the compounds of the invention bind effectively to the 18 kDa mitochondrial Translocator Protein (TSPO) and inhibit its cell death function.
• TSPO mitochondrial Translocator Protein
• these compounds have specific cell death preventing properties and thereby neuroprotective properties, both for neurons themselves as well as the glial cells maintaining neuron health.
• glutamate which is known to be an important agent causing secondary brain damage after traumatic brain damages, and also one of the toxic agents during neurodegeneration, the compounds of the present invention prevent cell death in the brain.
• These compounds also protect against other agents that are part of secondary brain damage and neurodegeneration as well as other pathological conditions (for example, but not restricted to, glutamate, glutamate receptor ligands other than glutamate, hypoxia mimicking agents, beta-amyloids, nitric oxide generating agents, apoptosis inducing agents, steroids, ammonium chloride, toxic compounds, interference with ATP production, etc.).
• agents that are part of secondary brain damage and neurodegeneration for example, but not restricted to, glutamate, glutamate receptor ligands other than glutamate, hypoxia mimicking agents, beta-amyloids, nitric oxide generating agents, apoptosis inducing agents, steroids, ammonium chloride, toxic compounds, interference with ATP production, etc.
• These compounds also activate differentiation of cells, making them useful to stimulate restorative processes in diseased and damaged CNS.
• the compounds of the invention are useful in treating and preventing diseases and conditions, including neurodegenerative diseases and brain injuries, associated with programme
• the compounds of the invention may be used in one or more of the following modes of action: 1) prophylactically; 2) counteracting progressing damage (e.g., brain damage) and disease (e.g., neurodegeneration); 3) stimulating self-repair; and 4) supporting replacement of diseased and damage tissue by grafts / transplants.
• progressing damage e.g., brain damage
• disease e.g., neurodegeneration
• stimulating self-repair e.g., neurodegeneration
• supporting replacement of diseased and damage tissue by grafts / transplants e.g., grafts / transplants.
• the broad applicability of the compounds presented herein is due to their ability to modulate processes common to the disease and progressing damage associated therewith (such as cell death) as well as their ability to differentiate specific cell types to the desired form of mature cells.
• the present invention provides a compound represented by the structure of formula (I):
• R 1 and R 2" are each independently a linear or branched C1-C12 alkyl; R is a halogen; and
• n 0, 1, 2, 3, 4 or 5;
• R 1 and R2 are each a 1 2
• R and R are different from each other.
• one of R 1 and R 2 is methyl, and the other is ethyl.
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are the same (thereby forming a symmetric amide), and n is other than 0 (i.e. the compound has one or more halogen substituents on the phenyl ring).
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are the same (thereby forming a symmetric amide), and n is other than 0 (i.e. the compound has one or more halogen substituents on the phenyl ring).
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are the same (thereby forming a symmetric amide), and n is other than 0 (i.e. the compound has one or more halogen substituents on the phenyl ring).
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are the same (thereby forming a symmetric amide), and n is other than 0 (i.e. the
• R 1 and R 2 are different from each other (thereby forming an asymmetric amide).
• R 1 and R 2 are different from each other.
• R 1 and R 2 may be the same (symmetric amides) or different from each other (asymmetric amides).
• the compound is represented by the structure of formula (I), wherein n is other than 0 and R 1 and R 2 are different from each other, thereby forming an asymmetric amide, having a halogenated phenyl substituent.
• the present invention teaches how to systematically improve the desired characteristics of therapeutically active compounds, including but not restricted to protection against cell death induced by glutamate in cell culture, by specific, targeted modifications in the structures of the compounds of the invention (i.e. modifications of the alkyl side chains and halogenations, CI for example, of the rotatable phenyl ring).
• Representative compounds of the invention are selected from compounds 1, 2, 3, 4, 5, 6, 7 and 8, with compound 1, compound 5 and compound 6 being currently preferred.
• the structures of compounds 1 to 8 are provided in the detailed description hereinbelow.
• the present invention provides a pharmaceutical composition
• a pharmaceutical composition comprising a pharmaceutically acceptable carrier, and as an active ingredient a compound represented by formula (I) or a compound of any of formulae 1, 2, 3, 4, 5, 6, 7 or 8.
• the described pharmaceutical composition is in a form suitable for oral, parenteral, transdermal, topical, or rectal administration, administration by inhalation, administration via a suppository or administration via dialysis.
• oral, parenteral, transdermal, topical, or rectal administration administration by inhalation, administration via a suppository or administration via dialysis.
• the compounds of the invention are unexpectedly more potent than the compounds disclosed in US 8,541,428, as well as classical TSPO ligands, as they combine effective TSPO binding with good protection against cell death, as for example demonstrated herein in cell culture.
• compound 1 of the present invention enhances lifespan and improves locomotor activity of transgenic R6-2 mice, an animal model for the neurodegenerative motor disease of Huntington, while Compound A, the most potent compound described in US 8,541,428, as well as the classical TSPO ligand, PK 11195, had no such effects in this animal model. Halogenation of the compounds appears to be very effective as demonstrated by robust lifespan enhancing and tremor reducing effects of compound 5 and compound 6 in the R6-2 transgenic mouse model for the human neurodegenerative disease of Huntington disease.
• a compound comprising an asymmetric amide is more potent than its counterparts having symmetric amides.
• the asymmetry is important both for the combination of affinity to TSPO and the capability to prevent cell death, as well as counteracting neurodegenerative processes in both animals and man. None of the compounds described in US 8,541,428 have an asymmetric amide moiety. The discovery of the combined effect of the asymmetric amides, which contributes both to the affinity of the compounds to TSPO as well as their ability to prevent cell death, and to ameliorate neurodegenerative processes, could not have been foreseen.
• halogen substituent(s) on the phenyl ring substantially affect the combination of binding properties and cell protective functions of the novel compounds described by formula (I).
• US 8,541,428 does not exemplify any halogenated derivatives nor any asymmetric amides at all.
• quinazoline derivatives of formula (I), which comprise at least one halogen moiety on the phenyl ring, and/or an asymmetric amide present constitute additional embodiments of the present invention.
• halogens located on the rotatable phenyl ring in interaction with these moieties can affect binding to the TSPO as well as control of TSPO function, including its regulation of cell death.
• halogen(s) on the rotatable phenyl ring does not necessarily significantly affect binding to TSPO, however, the presence of halogens prevents or reduces undesired lethal effects in cell culture induced by high concentrations (100 ⁇ ) of compound of the same structure but lacking the halogenation. Furthermore, the protective effects of halogenated compounds against glutamate-induced cell death are better than or equal to those of the corresponding the non-halogenated analogs. As such, halogenated compounds present a particular advantage in that they can be more protective and are less toxic and may be associated with a reduced side effect profile as compared with non-halogenated derivatives.
• Another advantage of the compounds of the present invention is that they are stable over several months of storage, with little batch to batch variation in properties.
• TSPO knockdown led to major changes in gene expression of glutamate receptors, transporters, and enzymes in human astrocyte type cells (Veenman et al., 2012b).
• short term treatment with the compounds of the present invention may prevent cell death by blocking collapse of the mitochondrial membrane potential ( ⁇ ), while long term treatment, as for example achieved by stable knockdown of TSPO expression, or by chronic inhibition with TSPO ligands, can regulate gene expression to counteract neurodegenerative processes.
• the present invention includes the capability of the compounds described herein to affect mitochondrial regulated cell processes, as well as gene expression in relation to brain damage in the aftermath of brain trauma, as well as in relation to neurodegenerative diseases (Bode et al. 2012; Veenman et al. 2012).
• the compounds or pharmaceutical compositions described above are useful for a variety of modes of treatment.
• the compound or pharmaceutical composition is given as prophylactic treatment to subjects in high risk groups including: subjects engaging in high risk activities, an infant or child at risk for developing a neurodevelopmental disorder associated with brain damage and/or neurodegeneration, a subject at risk for having a hereditary disease associated with brain damage and/or neurodegeneration, and/or a subject at risk for developing age related neurological diseases associated with brain damage and/or neurodegeneration.
• the compound or pharmaceutical composition is given during injury or disease progression as a counteracting treatment.
• the compound or pharmaceutical composition is given as, or in addition to, counteracting treatment in order to stimulate self- repair processes in the brain.
• the compound or pharmaceutical composition is given as treatment to support transplants of cells and tissue repair in a subject in need thereof. Each possibility represents a separate embodiment of the present invention.
• the present invention provides a method for treating or preventing brain damage and/or progression and/or symptoms thereof, by administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the brain damage is due to brain injury resulting from an acute event such as traumatic brain injury (TBI).
• TBI traumatic brain injury
• the brain damage is due to a secondary brain damage resulting from TBI.
• the brain damage is a secondary brain damage resulting from agents that are involved in secondary brain damage or neurodegeneration, for example glutamate, glutamate receptor ligands other than glutamate, hypoxia mimicking agents, nitric oxide generating agents, apoptosis inducing agents, steroids, ammonium chloride, toxic compounds, and agents which interfere with ATP production.
• the brain damage is due to an acute or chronic challenge resulted from an infection, exposure to toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs. Each possibility represents a separate embodiment of the present invention.
• the present invention further provides a method for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion, and/or differentiation, to thereby replenish depleted brain cells in a subject having brain damage due to injury, or who is at risk for developing brain damage due to injury, by administering to the subject an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention further provides a method of treating or preventing a neurodegenerative disease or a symptom thereof, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention provides a method for preventing neurodegeneration by preventing programmed cell death in a subject having a neurodegenerative disease or who is at risk for developing a neurodegenerative disease, comprising the step of administering to the subject an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention provides a method for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion, and/or differentiation, to thereby replenish depleted brain cells in a subject having a neurodegenerative disease or who is at risk for developing a neurodegenerative disease, comprising the step of administering to the subject an effective amount of a compound or a pharmaceutical composition as described above.
• the neurodegenerative disease is selected from the group consisting of Alzheimer's Disease, Parkinson's Disease, Huntington disease, Lou Gehrig Disease, Multiple Sclerosis, autoimmune disorders, Pick Disease, diffuse Lewy body disease, progressive supranuclear palsy (Steel-Richardson syndrome), multisystem degeneration (Shy-Drager syndrome), motor neuron diseases, amyotrophic lateral sclerosis, degenerative ataxias, cortical basal degeneration, ALS-Parkinson's-Dementia complex of Guam, subacute sclerosing panencephalitis, synucleinopathies, primary progressive aphasia, striatonigral degeneration, Machado-Joseph Disease/spinocerebellar ataxia type 3 and olivopontocerebellar degenerations, Gilles De La Tourette disease, bulbar and pseudobulbar palsy, spinal and spinobulbar muscular atrophy (Kennedy Disease), primary lateral sclerosis, familial spa
• the present invention further provides a method of stimulating or enhancing restorative processes in the CNS, including migration of progenitor cells to damaged brain areas, neurodifferentiation in damaged brain areas, and re-establishment of damaged neurocircuitry, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention provides a method for preventing, reducing or treating brain edema, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the treated brain edema results from traumatic brain injury (TBI), a neurodegenerative disease or an acute or chronic challenge selected from infection, toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs.
• TBI traumatic brain injury
• a neurodegenerative disease or an acute or chronic challenge selected from infection, toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs.
• the present invention provides a method for promoting grafting or transplant of cells, tissues and/or organs, comprising the step of contacting such cells, tissues and/or organs with an effective amount of a compound or a pharmaceutical composition as described above.
• the cells, tissues and/or organs are grafted or transplanted in a subject in need thereof.
• the cells, tissues and/or organs are grafted or transplanted in the CNS of a subject in need thereof.
• the cells, tissues and/or organs are brain related. Each possibility represents a separate embodiment of the present invention.
• the present invention further provides a method for promoting brain development in a subject having a developmental disorder or who is at risk for having a developmental disorder, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the developmental disorder is selected from the group consisting of Huntington disease, trisomy 21, Fragile X syndrome, Rett syndrome, Williams syndrome, pediatric autoimmune neuropsychiatric disorders associated with Streptococcal infection, Sydenham's chorea, toxoplasmosis, neurosyphilis, subacute sclerosing panencephalitis, schizophrenia, autism or developmental disorders caused by blindness, deafness, (sensory deprivation), metabolic disorders (e.g. diabetes, phenylketonuria), nutritional deficits (e.g. spina bifida, anencephaly, fetal alcohol syndrome), congenital injury or injury occurring in infancy or childhood.
• Each possibility represents a separate embodiment of the present invention.
• the present invention provides a method for repairing lesions and/or stimulating repair of the spinal cord by inducing axon growth across damaged areas and into their target area, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention provides a method for preventing or treating a cardiovascular disease, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the cardiovascular disease results from or is associated with Huntington Disease.
• the cardiovascular disease is selected from the group consisting of heart failure, congestive heart failure, cardiac arrest, and myocardial infarction. Each possibility represents a separate embodiment of the present invention.
• the compound or composition is administered prophylactically to a subject who is at risk of developing brain damage and/or neurodegeneration.
• said subject is at risk for sustaining brain injury or for developing a neurodegenerative disease, brain edema or a cardiovascular disease, or the subject is a transplant candidate.
• the compound or composition is administered prophylactically to a subject selected from the group consisting of: a subject engaging in high risk activities, an infant or child at risk for developing a neurodevelopmental disorder associated with brain damage and/or neurodegeneration, a subject at risk for having a hereditary disease associated with brain damage and/or neurodegeneration, and a subject at risk for developing age related neurological diseases associated with brain damage and/or neurodegeneration.
• a subject engaging in high risk activities an infant or child at risk for developing a neurodevelopmental disorder associated with brain damage and/or neurodegeneration
• a subject at risk for having a hereditary disease associated with brain damage and/or neurodegeneration and a subject at risk for developing age related neurological diseases associated with brain damage and/or
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for the treatment or prevention of brain damage and/or progression and/or symptoms thereof.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion, and/or differentiation, to thereby replenish depleted brain cells in a subject having brain damage due to injury or who is at risk for developing brain damage due to injury.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for treating or preventing a neurodegenerative disease or a symptom thereof.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for preventing programmed cell death in a subject having a neurodegenerative disease, or who is at risk for developing a neurodegenerative disease.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion, and/or differentiation, to thereby replenish depleted brain cells in a subject having a neurodegenerative disease, or who is at risk for developing a neurodegenerative disease.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for stimulating or enhancing restorative processes in the CNS, including migration of progenitor cells to damaged brain areas, neurodifferentiation in damaged brain areas, and re-establishment of damaged neurocircuitry.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for treating or preventing brain edema.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for promoting grafting or transplant of cells, tissues and/or organs.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for promoting brain development in a subject having a developmental disorder or who is at risk of having a developmental disorder.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for repairing lesions and/or stimulating repair of the spinal cord by inducing axon growth across damaged areas and into their target area in a subject in need thereof.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for treating or preventing a cardiovascular disease entailing heart failure.
• the described cardiovascular disease results from or is associated with Huntington Disease.
• FIG. 1 Compound 1 (Cpd 1) (characterized by methyl and ethyl as alkyl groups) significantly reduces glutamate-induced cell death levels in culture of U118MG cells of neural origin (glutamate can act as a neurotoxin that has cell damaging effects in case of brain injury and neurodegeneration). This protection includes cell death processes under the control of the TSPO i.e. collapse of the mitochondrial potential ( ⁇ ) and cardiolipin oxidation are prevented.
• Fig 1A cell death induced by glutamate is dose dependently reduced by Cpd 1. Importantly, Cpd 1 has only very little lethal effect by itself. ### p ⁇ 0.001 vs. vehicle control (control), * p ⁇ 0.05 vs.
• Fig. IB ⁇ collapse induced by glutamate is prevented by Cpd 1. ## p ⁇ 0.01 vs. vehicle control (cont), ** p ⁇ 0.01 vs. glutamate exposed (glut); Fig 1C: ROS generation at mitochondrial levels induced by glutamate is attenuated by Cpd 1. ### p ⁇ 0.001 vs. vehicle control (cont), *** p ⁇ 0.001 vs. glutamate exposed cells (glut).
• FIG. 5 Effects of Compound 1 in an animal model.
• Compound 1 extends the life span of R6-2 mice, a transgenic model of Huntington Disease compared to vehicle treated (DMSO) and sham treated (saline) R6-2 mice.
• R6-2 mice present a transgenic mouse model for the neurodegenerative disease of Huntington. No difference is found between DMSO treated and saline treated R6-2 mice.
• B) The comparative prior art Compound A has no effect in this Huntington disease model, as compared to DMSO and saline treated R6-2 mice.
• the classical TSPO ligand PK 11195 has no effect at all in this Huntington disease model (data not shown).
• FIG. 6 Compound 1 enhances locomotor activity of R6-2 mice compared to vehicle treated (DMSO shown in the figure) R6-2 mice and sham treated (saline not shown in the figure) R6-2 mice.
• the comparative prior art Compound A has no effect at all in this Huntington disease model (data not shown).
• the classical TSPO ligand PK 11195 has no effect at all in this Huntington disease model (data not shown).
• Figure 7 Functional characteristics of compounds 1, 5, 6, 7, 8 of the present invention in comparison to compound A.
• "Moderate" affinity provided by alkyl modifications provides optimal protection (Cpd 1 and Cpd 6).
• Addition of halogen to the rotatable phenyl improves protection and reduces lethal effects at "high" concentrations (100 ⁇ ) (Cpd 5, 6, 8).
• Additional elongation of alkyl side chains provides relatively strong affinity for the TSPO (Cpd 7, 8).
• Production of derived compounds combining alkyl modifications and halogenation further enhances protective effects to glutamate, e.g., Cpd 6 in comparison to all other compounds in this scheme.
• Figure 8 Protection against cell death induced by glutamate in Ul 18MG cells by compounds 1, 5, 6, 7, 8 of the present invention.
• Figure 8A Reference Cpd A (US Pat. No. 8,541,428).
• Figure 8B Cpd 5.
• Figure 8D Cpd 6.
• Figure 8E Cpd 7.
• Figure 8F Cpd 8.
• Figure 9 Reduction of adverse, undesired cell death effects in culture.
• Undesired lethal effects of the compounds of the invention are minimized by halogenation.
• This summary shows that halogenation with CI reduces lethal effects at a concentration of 100 ⁇ , i.e. only the protective characteristics remain.
• Cpd 5 shows the least adverse side effects.
• FIG. 10 In cell culture, given together with glutamate, Cpd 1 can induce differentiation of PC 12 cells toward neuronal type cell as characterized by outgrowth of neurites resembling axons. Other compounds of the present invention have the same effects (while classical TSPO ligands reportedly are not effective regarding this kind of differentiation).
• the triangles point at neurites resembling axons as induced by application of Cpd 1.
• the arrows point at potential contact sites.
• Figure 11 X-ray determined ball and stick structure of compound 1.
• Figure 12 Compound 6 (a halogenated derivative of compound 1) extends the life span of R6-2 mice compared to vehicle treated (DMSO) R6-2 mice.
• the vertical lines represent the median lifespan in each treatment group.
• FIG. 13 Compound 5 (a halogenated derivative of compound A) extends the life span of R6-2 mice compared to vehicle treated (DMSO) R6-2 mice.
• the vertical lines represent the median lifespan in each treatment group.
• the present invention relates to novel heterocyclic compounds including a quinazoline scaffold preferably having a set of asymmetric alkyl chains attached to the amide group of formula (I), and/or various halogens attached to the rotatable phenyl ring in formula (I), as well as individual compounds represented by the structure of formulas 1, 2, 3, 4, 5, 6, 7 and 8, as described herein.
• Compounds of the invention, in particular compound 1 bind effectively to the TSPO and regulate its cell death function, and may also regulate the role of TSPO role in gene expression, cell cycle, migration, proliferation, differentiation, and related tissue development and repair.
• the invention includes the pharmaceutical compositions containing the compounds, and their therapeutic use in treating and preventing brain damage resulting from acute events [e.g., traumatic brain injuries (TBI) and its after effects], chronic events (e.g., infection, toxins and/or drug use), and neurodegenerative diseases, and associated disorders.
• acute events e.g., traumatic brain injuries (TBI) and its after effects
• chronic events e.g., infection, toxins and/or drug use
• neurodegenerative diseases, and associated disorders e.g., neurological disorders, neurological disorders.
• the present invention provides a compound represented by the structure of formula (I):
• R 1 and R 2" are each independently a linear or branched Ci-C 12 alkyl; R is a halogen; and
• n 0, 1, 2, 3, 4 or 5;
• R 1 and R 2 are each a C 1 -C4 alkyl.
• the compound is represented by the structure of formula (I), wherein R 1 and R are each independently selected from the group consisting of methyl, ethyl, propyl, isopropyl, n-butyl, sec -butyl, isobutyl and tert-butyl.
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are the same (thereby forming a symmetric amide), and n is other than 0.
• the compound is represented by the structure of formula (I), wherein R 1 and R 2 are different from each other (thereby forming an asymmetric amide). According to the principles of the present invention, when n is 0 (i.e. R 3 does not exist), R 1 and R2 are different from each other.
• R 1 and R 2 may be the same (symmetric amides) or different from each other (asymmetric amides).
• n in the compounds of formula (I) is 0. In another preferred embodiment, n in the compounds of formula (I) is 1. In another preferred embodiment, n in the compounds of formula (I) is 2. Other possibilities include n is 3, 4, or 5. Currently preferred R groups are CI, Br or F, or a combination thereof. Each possibility represents a separate embodiment of the present invention. Representative compounds of the invention are selected from compounds 1,2,3, 4, 5, 6, 7, and 8 and are presented below.
• a compound comprising an asymmetric amide is more potent than its counterparts having symmetric amides.
• compounds of formula (I) wherein R 1 is different from R are currently preferred.
• incorporation of halogen(s) on the rotatable phenyl ring prevents or reduces undesired lethal effects in cell culture induced by high concentrations of compound of the same structure but lacking the halogenation.
• compounds of formula (I) which contain one or more halogens on the phenyl ring are currently preferred.
• the present invention provides a pharmaceutical composition
• a pharmaceutical composition comprising a pharmaceutically acceptable carrier, and as an active ingredient a compound represented by formula (I) or selected from any one of compounds 1, 2, 3, 4, 5, 6, 7 and 8.
• alkyl refers to any saturated aliphatic hydrocarbon, including straight-chain and branched-chain groups.
• the alkyl group has 1-12 carbons designated here as Ci-Ci2-alkyl.
• the alkyl group has 1-6 carbons designated here as Ci-C 6 -alkyl.
• the alkyl group has 1-4 carbons designated here as Ci-C4-alkyl.
• alkyl groups include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, pentyl including all its isomers, hexyl including all its isomers, heptyl including all its isomers, octyl including all its isomers, nonyl including all its isomers, decyl including all its isomers, undecyl including all its isomers, and dodecyl including all its isomers.
• alkylene denotes a bivalent radical where the alkyl radical is bonded at two positions connecting together two separate additional groups (e.g., CH 2 ).
• the alkyl group can be unsubstituted, or substituted with one or more substituents selected from the group consisting of halogen, hydroxy, alkoxy, aryloxy, alkylaryloxy, heteroaryloxy, oxo, cycloalkyl, phenyl, heteroaryl, heterocyclyl, naphthyl, amino, alkylamino, arylamino, heteroarylamino, dialkylamino, diarylamino, alkylarylamino, alkylheteroarylamino, arylheteroarylamino, acyl, acyloxy, nitro, carboxy, carbamoyl, carboxamide, cyano, sulfonyl, sulfonylamino, sulfinyl, sulfinylamino, thiol, Q to Ci 2 alkylthio arylthio, or Ci to C 12 alkylsulfonyl groups. Any substitu
• halogen as used herein alone or as part of another group refers to chlorine (CI), bromine (Br), fluorine (F), and iodine (I).
• One or more of the compounds of the invention may be present as a salt.
• salt encompasses both basic and acid addition salts, including but not limited to salts of amine nitrogens with organic or inorganic acids.
• Such acids include hydrochloric, hydrofluoric, trifluoroacetic, sulfuric, phosphoric, acetic, succinic, citric, lactic, maleic, fumaric, palmitic, cholic, pamoic, mucic, D-glutamic, D-camphoric, glutaric, phthalic, tartaric, lauric, stearic, salicyclic, methanesulfonic, benzenesulfonic, sorbic, picric, benzoic, cinnamic, and like acids.
• the present invention also includes solvates of compounds of formula (I) and salts thereof.
• “Solvate” means a physical association of a compound of the invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances the solvate will be capable of isolation.
• “Solvate” encompasses both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like.
• “Hydrate” is a solvate wherein the solvent molecule is water.
• the present invention also includes polymorphs of compounds of formula (I) and salts thereof.
• polymorph refers to a particular crystalline state of a substance, which can be characterized by particular physical properties such as X-ray diffraction, IR spectra, melting point, and the like.
• Glia constitutes the vast majority of brain cells and serves to maintain and protect healthy neurons, and it is now realized that glia presents a critical factor in the progress of neurodegeneration.
• the present invention is the first to actually consider glia, including astroglia, microglia, and oligodendrocytes, as a major venue for treatment of brain damage due to injury and disease.
• ⁇ mitochondrial membrane potential
• the present invention targets the TSPO to treat brain damage associated with any type of brain injury, neurodegenerative diseases, and related conditions.
• the compounds of the invention bind effectively to TSPO in combination with the prevention of cell death in a way superior to present day available treatments, including prevention of programmed cell death, as it occurs due to brain injury and/or neurodegenerative diseases.
• the capability of the compounds of the invention to activate and facilitate repair properties regarding immune response, inflammation, cell proliferation, cell migration, cell differentiation, neurite outgrowth, etc. enables application for curative treatment of various types of brain damage, as described above and also due to excessive drug use, toxic challenges, infections, etc.
• the compounds of the invention prevent/reduce basal programmed cell death levels in neural cells i.e. glia and/or neurons, including attenuation of cell death induced by glutamate, which is known to be an important agent in processes underlying neurodegeneration and cell death and cell damage in the brain. Furthermore, these compounds prevent/reduce cell death induction by other agents that contribute to neurodegeneration and progression of brain damage. Thus, the compounds of the invention prevent cell death due to most if not all of the factors contributing to neurodegeneration, progressing brain trauma, and associated disorders. In addition, the compounds may contribute to repair of damaged brain areas by replenishing the damaged brain areas and reestablish damaged neurociruitry with new brain cells, including neurons, and stimulate their differentiation. As such, the compounds of the invention are useful in preventing, treating, and in addition to symptom amelioration, potentially providing actual cures for neurodegenerative diseases and progressing brain damage after brain injury.
• the compounds of the invention provide better protection than classical TSPO ligands against programmed cell death. Furthermore, in contrast to problems seen with classical TSPO ligands in cell culture, the compounds of the invention are not as toxic at high concentrations, or not toxic at all. Furthermore, the compounds of the invention do not enhance apoptotic levels of apoptosis inducers such as glutamate, as classical TSPO ligands may do (in particular at high concentrations > 50 ⁇ ) in cell culture. As such, the compounds of the invention are particularly advantageous as compared with classical TSPO ligands. In addition, they unexpectedly combine effective affinity to the TSPO with cell protective capacity, as compared with the compounds disclosed in US 8,541,428.
• the compounds of the present invention demonstrate favorable properties such as enhanced lifespan and increased locomotor activity, while classical TSPO ligands, and compounds disclosed in US 8,541,428, completely lack these positive effects in the R6-2 model. It was further found in a separate experiment that compound 5 and compound 6 reduced the incidence of tremor activity. These characteristics indicate that the compounds have substantial neuroprotective properties, including the pathological behavioral effects and other symptoms, including lethality, and thus can be used to treat and prevent secondary brain damage after brain injury, brain surgery, neurodegenerative diseases, and associated disorders, for example, brain damage due to infections, toxic challenges, and excessive drug use, including recreational, over the counter, and prescribed drugs.
• the compounds of the invention unexpectedly combine good affinity to the TSPO with very effective capability to reduce cell death levels in cells, in particular reduction of cell death levels by neurotoxic agents that are known to be an important agents causing secondary brain damage after traumatic brain injury, and also take part in neurodegenerative diseases. Furthermore, halogen(s) on the rotatable phenyl ring prevents or reduces undesired lethal effects in cell culture otherwise induced by high concentrations (100 ⁇ ) of compound of the same structure but lacking the halogenation. In these aspects the compounds of the invention are superior to known TSPO ligands. They are also superior to any compound presently available to treat such disorders.
• the compounds of the invention are useful in the treatment and prevention of brain damage of any type, including brain damage resulting from traumatic brain injury (TBI), and/or secondary brain injury due to TBI as associated with, e.g., warfare, terrorism, traffic accidents (e.g., motor and non-motor vehicle accidents, and the like), sports injuries, violent crimes, household accidents, child abuse, domestic violence, work related accidents, gunshot wounds, etc.
• TBI traumatic brain injury
• Other types of brain injury may be due to toxic challenges, infections of any kind, and excessive drug use including recreational, over the counter, or prescription drugs.
• the compounds can also be applied prophylactically in high risk situations associated with brain injury.
• the compounds of the invention are also useful in treating and preventing neurodegenerative diseases such as Alzheimer Disease, Parkinson Disease, Huntington disease, Lou Gehrig Disease, Multiple Sclerosis, autoimmune disorders, and others. Additional therapeutic indications are described hereinbelow.
• the present invention provides a method for treating or preventing a neurodegenerative disease or a symptom thereof, comprising the step of administering to a subject in need thereof an effective amount of a compound of the present invention as described herein, or a pharmaceutical composition comprising such compound.
• the present invention relates to use of any one of the compounds or pharmaceutical composition as described above in treating or preventing neurodegenerative diseases or a symptom thereof.
• the present invention provides a method for preventing neurodegeneration (e.g. by preventing cell apoptosis) in a subject with a neurodegenerative disease or a subject who is at risk for developing a neurodegenerative disease, comprising the step of administering to a subject in need thereof an effective amount of a compound of the present invention, as described herein.
• the present invention relates to use of any one of the compounds or pharmaceutical composition as described above in preventing neurodegeneration (e.g. by preventing cell apoptosis) in a subject with a neurodegenerative disease or who is at risk for developing a neurodegenerative disease.
• the present invention provides a method for preventing, reducing or treating CNS damage, by activating and facilitating replenishment of lost brain cells via the processes of migration, proliferation, differentiation and/or adhesion of the required cells into and in the CNS regions in question, as well as other required regulation of immune response and inflammation.
• the method comprises the step of administering to a subject in need thereof an effective amount of a compound of the present invention, as described herein, or a pharmaceutical composition comprising such compound.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion and/or differentiation, to thereby replenish depleted brain cells in a subject in need thereof.
• the subject has a neurodegenerative disease or is at risk for developing a neurodegenerative disease.
• neurodegenerative diseases of the central nervous system are referred to as neurodegenerative, indicating that they are characterized by gradually evolving, relentlessly progressive neuronal death occurring for reasons that are still largely unknown. As such, they are different from comparable conditions induced by infections, metabolic derangements, and intoxications.
• a considerable proportion of the disorders classed as neurodegenerative are genetic, with either dominant or recessive inheritance. Others, however, occur only sporadically as isolated instances in a given family, for which a cause is typically unknown. Classification of the degenerative diseases cannot be based upon any exact knowledge of cause or pathogenesis; their subdivision into individual syndromes rests on descriptive criteria based largely upon neuropathologic and clinical aspects.
• the compounds of the invention are designed to treat any form of brain damage, be it due to mechanical injury, disease, infections, toxic challenges, and excessive use of drugs including recreational, over the counter, or prescription drugs, or otherwise.
• neurodegenerative diseases in the context of the present invention include, but are not limited to, Alzheimer Disease, Parkinson Disease, Huntington Disease, Lou Gehrig Disease, Multiple Sclerosis, autoimmune disorders, Pick Disease, diffuse Lewy body Disease, progressive supranuclear palsy (Steel-Richardson syndrome), multisystem degeneration (Shy-Drager syndrome), motor neuron diseases, amyotrophic lateral sclerosis, degenerative ataxias, cortical basal degeneration, ALS-Parkinson-Dementia complex of Guam, subacute sclerosing panencephalitis, synucleinopathies, primary progressive aphasia, striatonigral degeneration, Machado-Joseph disease/spinocerebellar ataxia type 3 and olivopontocerebellar degenerations, Gilles De La Tourette Disease, bulbar and pseudobulbar palsy, spinal and spinobulbar muscular atrophy (Kennedy Disease), primary lateral sclerosis, familial spastic para
• the present invention provides a method for treating or preventing brain damage and/or progression and/or symptom thereof, comprising the step of administering to a subject in need thereof an effective amount of a compound or composition of the present invention, as described herein.
• the brain damage is due to brain injury resulting from an acute event, such as traumatic brain injury (TBI) and the subsequent progressing secondary brain injury resulting from TBI.
• TBI traumatic brain injury
• the present invention provides a method for preventing or treating progression of brain damage resulting from traumatic brain injury (TBI) or secondary brain damage resulting from TBI, by activating and facilitating replenishment of lost brain cells and reestablishment of neurocircuitry via the processes of migration, proliferation, differentiation, and adhesion of the required cells into the CNS regions in question, as well as other required regulation of immune response and inflammation.
• TBI traumatic brain injury
• the method comprises the step of administering to a subject in need thereof an effective amount of a compound of formula of the present invention as described herein.
• the present invention also relates to the use of a compound of the present invention, in the manufacture of a medicament for treating or preventing brain damage and/or symptoms and/or progression of brain damage.
• the brain damage can be caused, e.g., by any type of brain injury.
• the present invention further provides a method for preventing, reducing or treating CNS damage by activating migration, proliferation, adhesion, and/or differentiation, to thereby replenish depleted brain cells in a subject having brain damage due to injury, or who is at risk for developing brain damage due to injury, by administering to the subject an effective amount of a compound or a pharmaceutical composition as described above.
• the brain injury is due to an acute event, e.g., traumatic brain injury (TBI).
• TBI traumatic brain injury
• the brain damage is secondary brain damage resulting from TBI, which presents a continuing, chronic process.
• this relates to agents that are involved in secondary brain damage or neurodegeneration, such as glutamate, glutamate receptor ligands other than glutamate, hypoxia mimicking agents, nitric oxide generating agents, apoptosis inducing agents, steroids, ammonium chloride, toxic compounds, or agents which interfere with ATP production.
• the brain damage is due to a chronic challenge such as infection, toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs.
• the brain injury is traumatic brain injury (TBI).
• TBI traumatic brain injury
• the compounds of the invention are particularly useful for preventing and treating secondary brain damage resulting from TBI.
• the compounds of the invention are useful, e.g., for treating soldiers in the battlefield, especially soldiers who have suffered TBI.
• secondary brain damage due to TBI can be treated or prevented by administering the compounds of the invention to soldiers, for example by supplying paramedics and / or the soldiers in the battlefield or under threat/at the site of terrorist attack with the compounds of the invention, so that the compounds can be administered on site as soon as possible after the soldier has suffered TBI.
• the compounds of the invention are also useful for civilians who are victims of violent crimes, including but not limited to, terrorist attacks, and any other mishap that can cause brain damage. This may reduce the incidence of disability presently occurring in the aftermath of TBI suffered due to hostilities, including terrorist attacks, as well as other crimes, and accidents.
• the utility of the compounds of the invention is not limited to violent crimes such as warfare and terrorist attacks.
• the compounds of the invention are also useful for individuals suffering from brain injury due to domestic occurrences, such as traffic accidents (e.g., motor and non-motor vehicle accidents, and the like), sports injuries, work related accidents, household accidents, child abuse, domestic violence, gunshot wounds, etc, including their consequences such events such as disability and epilepsy.
• traffic accidents e.g., motor and non-motor vehicle accidents, and the like
• sports injuries work related accidents, household accidents, child abuse, domestic violence, gunshot wounds, etc, including their consequences such events such as disability and epilepsy.
• the compounds may be given prophylactically for sports with a very high incidence of CNS damage.
• the present invention provides a method of stimulating or enhancing restorative processes in the CNS, including migration of progenitor cells to damaged brain areas, neurodifferentiation in damaged brain areas, and re-establishment of damaged neurocircuitry.
• the method comprises the step of administering to a subject in need thereof an effective amount of a compound of the present invention, as described herein.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for stimulating or enhancing restorative processes in the CNS.
• Heart failure is a major cause of death in R6-2 mice, a mouse model of Huntington
• the present invention provides a method for preventing or treating a cardiovascular disease, in particular heart failure, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the cardiovascular disease results from or is associated with Huntington Disease.
• the present invention relates to the use of any one of the compounds or pharmaceutical composition as described above for treating or preventing a cardiovascular disease, in particular heart failure.
• the present invention provides a method for preventing, reducing or treating brain edema, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for treating or preventing brain edema.
• the brain edema may results from traumatic brain injury, a neurodegenerative disease and/or an acute or chronic challenge as described above.
• the compounds of the present invention support replacement of diseased and damage tissue by grafts / transplants.
• the present invention further provides a method for promoting grafting or transplant of cells, tissues and/or organs, by contacting such cells, tissues and/or organs with an effective amount of a compound or a pharmaceutical composition as described above.
• the grafted or transplanted cells' target area is the CNS.
• the cells, tissues and/or organs are brain related.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for promoting grafting or transplant of cells, tissues and/or organs.
• the present invention further provides a method for promoting brain development in a subject having a developmental disorder or who is at risk for having a developmental disorder, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the compound of the current invention can be used for treating developmental disorders, example of such developmental disorders in the context of the present invention include, but are not limited to: Huntington disease, trisomy 21, Fragile X syndrome, Rett syndrome, Williams syndrome, pediatric autoimmune neuropsychiatric disorders associated with Streptococcal infection, Sydenham's chorea, toxoplasmosis, neurosyphilis, subacute sclerosing panencephalitis, schizophrenia, autism or developmental disorders caused by blindness, deafness, (sensory deprivation), metabolic disorders (e.g. diabetes, phenylketonuria), nutritional deficits (e.g.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for promoting brain development in a subject having a developmental disorder or who is at risk of having a developmental disorder.
• the present invention provides a method for repairing lesions and/or stimulating repair of the spinal cord by inducing axon growth across damaged areas and into their target area, comprising the step of administering to a subject in need thereof an effective amount of a compound or a pharmaceutical composition as described above.
• the present invention further relates to the use of any one of the compounds or pharmaceutical composition as described above for repairing lesions and/or stimulating repair of the spinal cord.
• the compounds of the invention may be used in one or more of the following modes of action: 1) prophylactically; 2) counteracting progressing damage (e.g., brain damage) and disease (e.g., neurodegeneration); 3) stimulating self-repair; and 4) supporting replacement of diseased and damage tissue by grafts / transplants.
• the compound or composition as described above is administered prophylactically to a subject who is at risk of developing brain damage and/or neurodegeneration.
• said subject is at risk for sustaining brain injury or for developing a neurodegenerative disease, brain edema or a cardiovascular disease, or wherein the subject is a transplant candidate.
• the prophylactically administered compound or composition is given to a subject selected from the group consisting of: a subject engaging in high risk activities, an infant or child at risk for developing a neurodevelopmental disorder associated with brain damage and/or neurodegeneration, a subject at risk for having a hereditary disease associated with brain damage and/or neurodegeneration, and a subject at risk for developing age related neurological diseases associated with brain damage and/or neurodegeneration.
• a subject selected from the group consisting of: a subject engaging in high risk activities, an infant or child at risk for developing a neurodevelopmental disorder associated with brain damage and/or neurodegeneration, a subject at risk for having a hereditary disease associated with brain damage and/or neurodegeneration, and a subject at risk for developing age related neurological diseases associated with brain damage and/or neurodegeneration.
• the compound is represented by the structure of formula (I).
• the compound is represented by the structure of formula 1.
• the compound is represented by the structure of formula 2.
• the compound is represented by the structure of formula 3.
• the compound is represented by the structure of formula 4. In another preferred embodiment, the compound is represented by the structure of formula 5. In another preferred embodiment, the compound is represented by the structure of formula 6. In another preferred embodiment, the compound is represented by the structure of formula 7. In another preferred embodiment, the compound is represented by the structure of formula 8.
• Each possibility represents a separate embodiment of the present invention.
• administering refers to bringing in contact with a compound of the present invention. Administration can be accomplished to cells or tissue cultures, or to living organisms, for example humans. In one embodiment, the present invention encompasses administering the compounds of the present invention to a human subject.
• a “therapeutic” or “effective” treatment is a treatment administered to a subject who exhibits signs of pathology for the purpose of diminishing or eliminating those signs.
• a “therapeutically effective amount” or an “effective amount” of a compound of the invention is that amount of compound which is sufficient to provide a beneficial effect to the subject to which the compound is administered.
• effective treatments according to the present invention include, but are not limited to, reducing or ameliorating one or more symptoms of TBI or any of the neurodegenerative diseases described herein, increasing lifespan, increasing motor activity, increasing cognitive capabilities and other brain functions, and preventing the progression of TBI and/or neurodegenerative diseases.
• the compounds of the invention may be used to treat brain damage due to infections, toxic challenges, excessive drug use, being recreational, over the counter, or prescription, etc.
• Brain edema refers to a condition of accumulation of excess fluid in the intracellular or extracellular spaces of the brain.
• Brain edema in the context of the present invention can be a result of a traumatic brain injury (TBI), a neurodegenerative disease or an acute or chronic challenge selected from infection, toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs.
• TBI traumatic brain injury
• a neurodegenerative disease or an acute or chronic challenge selected from infection, toxins, and excessive drug use of recreational, over the counter, and/or prescription drugs.
• grafting and “transplant” refer to a section of tissue or a complete organ that is removed from its original natural site and transferred to a new position in the same person or in a separate individual.
• Cardiovascular disease refers to heart and blood vessel disease. Cardiovascular disease in the context of the present invention includes, but is not limited to heart failure, congestive heart failure, cardiac arrest, and myocardial infarction.
• the compounds of the present invention can be administered alone, it is contemplated that these compounds will be administered in a pharmaceutical composition containing the compound of the invention together with a pharmaceutically acceptable carrier or excipient.
• compositions of the present invention can be formulated for administration by a variety of routes including oral, rectal, transdermal, parenteral (subcutaneous, intraperitoneal, intravenous, intraarterial, transdermal, and intramuscular), topical, intranasal, rectally via a suppository, or via dialysis.
• Such compositions are prepared in a manner well known in the pharmaceutical art and comprise as an active ingredient at least one compound of the present invention as described hereinabove, and a pharmaceutically acceptable excipient or a carrier.
• pharmaceutically acceptable means approved by a regulatory agency of the Federal or a state government or listed in the U. S. Pharmacopeia or other generally recognized pharmacopeia for use in animals and, more particularly, in humans.
• the active ingredient is usually mixed with a carrier or excipient, which may be a solid, semi-solid, or liquid material.
• a carrier or excipient which may be a solid, semi-solid, or liquid material.
• the compositions are preferably suited for oral administration, in which case they can be in the form of tablets, pills, capsules, pellets, granules, powders, lozenges, sachets, cachets, elixirs, suspensions, dispersions, emulsions, solutions, syrups, aerosols (as a solid or in a liquid medium), ointments containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions, and sterile packaged powders.
• the carriers may be any of those conventionally used. The choice of carrier will be determined by the particular method used to administer the pharmaceutical composition. Some examples of suitable carriers include lactose, glucose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water and methylcellulose.
• Other pharmaceutical carriers can be sterile liquids, such as water, alcohols (e.g. ethanol) and lipid carriers such as oils (including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like), phospholipids (e.g.
• lecithin polyethylene glycols, glycerine, propylene glycol, or other synthetic solvents.
• Water is a preferred carrier when the pharmaceutical composition is administered intravenously.
• Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions.
• the formulations can additionally include lubricating agents such as talc, magnesium stearate, and mineral oil; wetting agents, anti-oxidants, surfactants, emulsifying and suspending agents; preserving agents such as methyl- and propylhydroxybenzoates; sweetening agents; flavoring agents, colorants, buffering agents (e.g. acetates, citrates or phosphates), disintegrating agents, moistening agents, antibacterial agents, antioxidants (e.g. ascorbic acid or sodium bisulfite), chelating agents (e.g. ethylenediaminetetraacetic acid), and agents for the adjustment of tonicity such as sodium chloride.
• lubricating agents such as talc, magnesium stearate, and mineral oil
• wetting agents such as anti-oxidants, surfactants, emulsifying and suspending agents
• preserving agents such as methyl- and propylhydroxybenzoates
• sweetening agents e.g. acetates, citrates or
• the principal active ingredient is mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention.
• a solid preformulation composition containing a homogeneous mixture of a compound of the present invention.
• the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills, and capsules.
• This solid preformulation is then subdivided into unit dosage forms of the type described above containing from, for example, 0.1 to about 500 mg of the active ingredient of the present invention.
• Solid dosage forms can be prepared by wet granulation, dry granulation, direct compression, and the like.
• the solid dosage forms of the present invention may be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action.
• the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former.
• the two components can be separated by an enteric layer, which serves to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release.
• enteric layers or coatings such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol, and cellulose acetate.
• compositions of the present invention include aqueous solutions, alcoholic solutions, suitably flavored syrups, aqueous or oil suspensions, and flavored emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as elixirs, and similar pharmaceutical vehicles.
• compositions for inhalation include solutions and suspensions in pharmaceutically acceptable aqueous or organic solvents, or mixtures thereof, and powders.
• the liquid or solid compositions may contain suitable pharmaceutically acceptable excipients as described above.
• the compositions are administered by the oral or nasal respiratory route for local or systemic effect.
• Compositions in preferably pharmaceutically acceptable solvents may be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device may be attached to a face mask tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions may be administered, preferably orally or nasally, from devices that deliver the formulation in an appropriate manner.
• transdermal delivery devices Such transdermal patches may be used to provide continuous or discontinuous infusion of the compounds of the present invention in controlled amounts.
• transdermal patches for the delivery of pharmaceutical agents is well known in the art.
• the composition is prepared for topical administration, e.g. as an ointment, a gel, a drop, or a cream.
• topical administration e.g. as an ointment, a gel, a drop, or a cream.
• the compounds of the present invention can be prepared and applied in a physiologically acceptable diluent with or without a pharmaceutical carrier.
• Adjuvants for topical or gel base forms may include, for example, sodium carboxymethylcellulose, polyacrylates, polyoxyethylene- polyoxypropylene-block polymers, polyethylene glycol, and wood wax alcohols.
• Alternative formulations include nasal sprays, liposomal formulations, immediate- release formulations, slow-release formulations, controlled-release formulations, delayed- release formulations and the like, as are known in the art.
• compositions are preferably formulated in a unit dosage form.
• unit dosage form refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.
• the active ingredient In preparing a formulation, it may be necessary to mill the active ingredient to provide the appropriate particle size prior to combining with the other ingredients. If the active compound is substantially insoluble, it ordinarily is milled to a particle size of less than about 200 mesh. If the active ingredient is substantially water soluble, the particle size is normally adjusted by milling to provide a substantially uniform distribution in the formulation, e.g. about 40 mesh.
• composition of the invention may be administered locally to the area in need of treatment; this may be achieved by, for example, and not by way of limitation, local infusion during surgery, topical application, e.g. in conjunction with a wound dressing after surgery, by injection, by means of a catheter, by means of a suppository, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material.
• the compounds may also be administered by any convenient route, for example by infusion or bolus injection, by absorption through epithelial linings (e.g. oral mucosa, rectal and intestinal mucosa, etc.), and may be administered together with other therapeutically active agents. Administration may be localized, preferably close to the affected, injured, or diseased area, but it may also be systemic. In addition, it may be desirable to introduce the pharmaceutical compositions of the invention into the central nervous system by any suitable route, including intraventricular and intrathecal injection; intraventricular injection may be facilitated by an intraventricular catheter, for example, attached to a reservoir. A targeted drug delivery system can be used, including nanotechnology. Pulmonary administration can also be employed, e.g. by use of an inhaler or nebulizer, and formulation with an aerosolizing agent.
• epithelial linings e.g. oral mucosa, rectal and intestinal mucosa, etc.
• Administration may be localized, preferably close to the affected, injured, or diseased
• an infusion pump may be used to administer a compound of the invention.
• a compound of the invention may be administered in combination with a biodegradable, biocompatible polymeric implant, which releases the compound over a controlled period of time at a selected site.
• preferred polymeric materials include poly anhydrides, polyorthoesters, polyglycolic acid, polylactic acid, polyethylene vinyl acetate, copolymers and blends thereof (See, Medical applications of controlled release, Langer and Wise (eds.), 1974, CRC Pres., Boca Raton, FL).
• a controlled release system can be placed in proximity of the therapeutic target, thus requiring only a fraction of the systemic dose.
• the pharmaceutical compositions may be formulated for parenteral administration (subcutaneous, intravenous, intraarterial, transdermal, intraperitoneal, or intramuscular injection) and may include aqueous and non-aqueous, isotonic sterile injection solutions, which can contain anti-oxidants, buffers, bacteriostats, and solutes that render the formulation isotonic with the blood of the intended recipient, and aqueous and non-aqueous sterile suspensions that include suspending agents, solubilizers, thickening agents, stabilizers, and preservatives.
• parenteral administration subcutaneous, intravenous, intraarterial, transdermal, intraperitoneal, or intramuscular injection
• parenteral administration subcutaneous, intravenous, intraarterial, transdermal, intraperitoneal, or intramuscular injection
• parenteral administration subcutaneous, intravenous, intraarterial, transdermal, intraperitoneal, or intramuscular injection
• parenteral administration subcutaneous, intravenous,
• Oils such as petroleum, animal, vegetable, or synthetic oils and soaps such as fatty alkali metal, ammonium, and triethanolamine salts, and suitable detergents may also be used for parenteral administration.
• the compositions may contain one or more nonionic surfactants.
• Suitable surfactants include polyethylene sorbitan fatty acid esters, such as sorbitan monooleate and the high molecular weight adducts of ethylene oxide with a hydrophobic base, formed by the condensation of propylene oxide with propylene glycol.
• parenteral formulations can be presented in unit-dose or multi-dose sealed containers, such as ampoules and vials, and can be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, water, for injections, immediately prior to use.
• sterile liquid carrier for example, water
• Extemporaneous injection solutions and suspensions can be prepared from sterile powders, granules, and tablets of the kind previously described and known in the art.
• the amount of a compound of the invention that will be effective in the treatment of a particular disorder or condition will depend on the nature of the disorder or condition, and can be determined by standard clinical techniques.
• in vitro assays may optionally be employed to help identify optimal dosage ranges.
• the precise dose to be employed in the formulation will also depend on the route of administration, and the seriousness of the disease or disorder, and should be decided according to the judgment of the practitioner and each patient's circumstances.
• a preferred dosage will be within the range of 0.01-1000 mg/kg of body weight, more preferably, about 0.1 mg/kg to about 100 mg/kg and even more preferably about 1 mg/kg to about 10 mg/kg.
• Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test bioassays or systems.
• anthranilamide reacts with benzaldehyde or a halogenated derivative thereof, to give 2-phenylquinazolin-4-ol or a halogenated derivative thereof (Intermediate A).
• the intermediate is treated with a base (e.g. a hydride such as sodium or potassium hydride) to prepare the corresponding alcoholate, followed by addition of a carbamoyl derivative (X is, e.g., a halogen, preferably CI), to yield the O-amidation product.
• a base e.g. a hydride such as sodium or potassium hydride
• X is, e.g., a halogen, preferably CI
• Different alkyl groups on the nitrogen atom of carbamoyl chloride can be prepared as desired.
• bases include, but are not limited to, hydrides (e.g. sodium or potassium hydride).
• Other suitable bases include, but are not limited to an organic base such as a tertiary amine selected from acyclic amines (e.g. trimethylamine, triethylamine, dimethylphenylamine, diisopropylethylamine and tributylamine), cyclic amines (e.g. N-methylmorpholine), and aromatic amines (e.g. dimethylaniline, dimethylaminopyridine, and pyridine).
• a tertiary amine selected from acyclic amines (e.g. trimethylamine, triethylamine, dimethylphenylamine, diisopropylethylamine and tributylamine), cyclic amines (e.g. N-methylmorpholine), and aromatic amines (e.g. dimethylaniline, dimethylaminopyridine, and pyridine).
• the reaction may be conducted in the presence or absence of a solvent.
• a solvent such as esters (e.g. ethyl acetate), ethers (e.g. dimethoxyethane, THF), chlorinated solvents (e.g. dichloromethane or chloroform), dimethylformamide (DMF), acetonitrile or toluene, or mixtures of these solvents with each other.
• the base is potassium hydride (KH) and the solvent is dimethoxyethane (DME).
• KH potassium hydride
• DME dimethoxyethane
• TSPO binding and Western blot protein analysis TSPO binding assays were utilized to determine whether the compounds of the invention are able to compete with standard TSPO ligands, according to methods described previously (Veenman et al. 2004; Levin et al. 2005), and are used for quality control. Briefly, the assay utilizes radioactive standard ligand [ 3 H] PK 11195 (l-(2-chlorophenyl)-N-methyl-N-(l-methyl-propyl)-3 isoquinohnecarboxamide), and measures the ability of the compounds of the invention to displace standard ligand in binding to TSPO.
• radioactive standard ligand [ 3 H] PK 11195 (l-(2-chlorophenyl)-N-methyl-N-(l-methyl-propyl)-3 isoquinohnecarboxamide
• Radioactive binding was determined by measuring the radioactivity of the radioactive ligand-receptor complex, obtained in the reaction of radioactive standard with the TSPO. Radioactive standard and the examined compound were added to the TSPO and after some incubation time the radioactivity of the resulting ligand-receptor complexes was measured with the help of a ⁇ -counter. This radioactivity indicates the binding of the radioactive standard to TSPO. When the examined compound binds better to TSPO, consequently, less radioactive standard binds to TSPO, therefore the measured radioactivity is lower.
• IC50 half maximal inhibitory concentration
• Ki the calculated binding affinity of the novel compound to the TSPO.
• the protective capabilities of the compounds of the invention and involvement of the TSPO were tested in cell culture, using cell lines of glial and neuronal type, cultured and testing according to standard methods (Kugler et al. 2008; Zeno et al. 2009; Dadon- Nachum et al. Stem Cell Rev. 2011 ;7:664-71). Furthermore, primary cell cultures are also used (Banker, G. et al. Culturing Nerve Cells, 2nd Edition, The MIT Press (1998).
• Treatments included: (1) exposure to glutamate (35 mM), Abeta(l-42), NH 4 C1, C0CI 2 , and nitric oxide (NO) donors, in order to mimic particular aspects of neurodegeneration; (2) treatment with the novel compounds and with traditional TSPO ligands as comparative treatment; and (3) TSPO knockdown by genetic manipulation.
• Data are expressed as means ⁇ SD or SEM as appropriate.
• One-way or multiple analysis of variance, including post-hoc tests, as appropriate, are used to analyze the data.
• Bartlett's test for homogeneity of variance is used to determine the appropriate model i.e. parametric or non-parametric.
• Statistical significance is defined at p ⁇ 0.05.
• R6-2 model for Huntington Disease was chosen to test the effectiveness of Compound 1 and to compare the effectiveness of Compound 1 with those of the compound of US 8,541,428 (Cpd A) and with the classical TSPO ligand PK 11195, as well as other compounds disclosed herein.
• Compounds 5 and 6 were also tested in the same R6-2 model.
• Binding to TSPO was evaluated using a [ H]PK 11195 radio-assay, as described above. Results of binding to TSPO and protective effects of the compounds of the present invention are provided in Table 1. Compounds 1-8 were tested in cell culture, and favored compounds also in animal models (i.e. Compound 1). The structures of these compounds are provided hereinabove. Table 1 also includes the compound encompassed by US 8,541,428 (compound A).
• halogens attached to the rotatable phenyl ring linked to the quinazoline scaffold appears to improve cell protective effects, while retaining TSPO binding affinity (Figs. 2 - 4 and 7 - 9). It is therefore contemplated that attachment of halogen substituents to the compounds of formula (I) allows for further improvements, most notably regarding their capability to protect against cell death.
• Figures 7 to 9 show that halogenation at the phenyl ring does not necessarily affect TSPO affinity, but does have positive effects regarding protection.
• halogenation may be beneficial for the capability of the compounds of the present invention to protect the cells from cell death. This additional finding was completely unexpected and represents a separate embodiment of the present invention.
• compounds having a combination of asymmetrical amides and halogenated rotatable phenyls may have enhanced properties, combining the advantages conferred by both the asymmetrical amide and the halogen components.
• the compounds of the invention protect against cell death induced by glutamate in cell culture of Ul 18MG cells of astrocytic origin ( Figures 1, 2, 3 and 8) or SH SY 6Y cells of neuronal origin ( Figure 4).
• Figure 1 shows that Compound 1, which has good affinity for the TSPO (Table 1), prevents cell death and cell death processes induced by glutamate.
• Compound 1 of the present invention protects against glial cell death induced by 35 mM of glutamate in cell culture (Results are depicted as AVG ⁇ SD). In the control group, 35 mM of glutamate killed -60% of the cells.
• Compound 1 at concentrations from 10 - 100 ⁇ significantly protects against cell death induced by glutamate in glial cell culture.
• Compound 1 prevents collapse of the mitochondrial membrane potential ( ⁇ ), which is typically induced by glutamate and is normally under control of the TSPO (Results are depicted as AVG ⁇ SD).
• Compound 1 is very effective at a concentration of 25 ⁇ in this model (in order to achieve the same effect with the classical TSPO ligand PK 11195 a concentration of 50 ⁇ has to be applied (data not shown).
• FIG. 1C shows that Compound 1 prevents ROS generation at mitochondrial levels otherwise induced by glutamate (Results are depicted as AVG ⁇ SD).
• Figure 2 shows that Compound 2, which has moderate affinity for TSPO (Table 1), has strong protective properties in vitro against glutamate induced cell death in U118MG cells. Results are depicted as AVG ⁇ SEM. ***: One way ANOVA gave p ⁇ 0.0001; Bonferroni Multiple Comparisons Test gave p ⁇ 0.001 for glutamate vs. glutamate + compound 2 (5 - 50 ⁇ ). In figure 2, the white columns present exposure to glutamate without (0) or with Cpd 2 (1, 5, 10, 25, 50 ⁇ ). The black columns present treatment with only Cpd 2.
• ROS reactive oxygen species
• Figure 3 shows that Compound 3, which has affinity for TSPO (Table 1), has good protective properties in vitro against glutamate induced cell death in U118MG cells. Results are depicted as AVG ⁇ SEM. ***: One way ANOVA gave p ⁇ 0.0001 ; Bonferroni Multiple Comparisons Test gave p ⁇ 0.001 for glutamate vs. glutamate + compound 3 (5 - 50 ⁇ ). In figure 3, the white columns present exposure to glutamate without (0) or with Cpd 3 (1, 5, 10, 25, 50 ⁇ ). The black columns present treatment with only Cpd 3.
• Compound A differs from Compound 1 in that it has two methyl side chains (i.e., symmetric amide) instead of the one methyl and the one ethyl in Compound 1 (i.e., asymmetric amide).
• Compound 5 differs from Compound 1 in that it has two methyl side chains instead of one methyl and one ethyl, and a CI substituted to the 2 position of the third (rotatable) phenyl.
• Compound 6 differs from Compound 1 in that it has a CI substituted to the 2 position of the third (rotatable) phenyl.
• Compound 7 differs from Compound 1 in that it has two ethyl side chains instead of one methyl and one ethyl.
• Compound 8 differs from Compound 1 in that it has two ethyl side chains instead of one methyl and one ethyl, and a CI substituted to the 2 position of the third (rotatable) phenyl.
• FIG. 8A-F show activities of Reference Compound A (Fig. 8A)and Compounds 5, 1, 6, 7 and 8 of the invention (Fig. 8B-F, respectively).
• Control Cell death levels are taken as 0%
• Cpd 5 By itself, Cpd 5 does not induce cell death levels over 5%.
• Glu + Cpd 5 When Cpd 5 is given as a treatment to counteract cell death induced by glutamate, increasing concentrations of Cpd 5 reduce cell death levels from over 50% to less than 5%.
• Compounds 1, 5 and 6 appear to be superior over Compounds A (US Pat. No. 8,541,428), 7, and 8 in that they protect better and more consistently at the concentrations of 10, 25, 50, and 100 ⁇ against glutamate induced cell death.
• compound 6 appears to be superior over compound 1 in that in general it protects better at concentrations of 10, 25, 50, and 100 ⁇ against glutamate induced cell death.
• Compound 5 appears to be superior over the rest of the compounds in the current experiment.
• the desired effects are of the compounds are: i) minor lethal effects of the compounds themselves, and ii) consistent reductions of cell death levels otherwise induced by glutamate.
• Compound 1 is absorbed from the site of the i.p. administration, good half-life in the mouse of 3.5 hrs.
• DMSO/Sesame oil (1:9) or DMSO alone show no adverse effects in mice and rats.
• Sesame oil could induce hair loss around the ears of the mice in question. Therefore, the vehicle of choice is pure DMSO, since it has no such or other adverse effect, when injected in small quantities of 10 - 20 ⁇ in mice and around 200 ⁇ in rats, injected daily over periods of several weeks to several months.
• Example 5 Superior Lifespan Extending Effects of Compound 1 in R6-2 mice. a transgenic mouse model for Huntington disease
• R6-2 is a transgenic mouse model for Huntington Disease. Huntington Disease in humans is a hereditary neurodegenerative disease affecting motor performance and finally leading to death of the patients having the disease.
• R6-2 mice (-120 CAG repeats B6CBA- Tg(HDexonl)62Gpb/3J) mice were used for the experiments described below. For this purpose, mice obtained from the Jackson Laboratory (ME) were bred. In particular: males genotype: Hemizygous for Tg(HDexonl)62Gpb, and females from the wildtype background strain B6CBAF1/J were bred according to the protocol of Jackson Laboratory. The male heterozygote offspring (R6-2 mice) were used for determining life span and motor activity, with and without drug treatment.
• Drug treatment consisted of daily (5 continuous days a week) subcutaneous injections of mice with 20 ⁇ of: saline (sham control), vehicle DMSO (vehicle control), classic TSPO ligand PK 11195 (15 mg/kg), prior art Compound A (15 mg/kg), Compound 1 (15 mg/kg), and compound 1 (7.5 mg/kg). Drug treatment started on week 5 after birth. Quantities were used according to previous studies regarding TSPO ligands.
• mice were observed daily, and the day of death was noted.
• Behavioral experiments included distance covered in an open field apparatus, and tremor measurements.
• the open field is made of a black lusterless Perspex box (50L X 50W X 40H cm) placed in a dimly lit room (50 lx), its floor made from white Perspex to achieve visual contrast with black mice. Mice are placed in the corner of the open field (facing the wall) and then given 5 min of free exploration.
• the behavior is videotaped by a CCTV Panasonic camera and post-recording analysis with Ethovision XT 7.0 software (Noldus, The Netherlands), according to: Lemoine et al. Pharmacology Biochemistry and Behavior. 1990;36:S5-SS.
• Tremor apparatus To assay incidence of tremor in R6-2 mice of the experiments for this invention, the StartleMonitor of Kinder Scientific (Poway, CA), with specific software to assay and present force pressure changes in newtons, was used.
• Figure 5A shows that Compound 1 increases average lifespan in R6-2 mice.
• Compound 1 increases the average lifespan compared to sham and vehicle treated mice of the transgenic R6-2 model for Huntington Disease.
• the effects of saline and DMSO are indistinguishable from each other.
• onset of death events is later and the average lifespan is longer for R6-2 mice treated with Compound 1, which is considerably and significantly favorable as compared with control R6-2 mice.
• the y-axis presents the percentage of surviving animals per week.
• the x-axis presents the number of weeks from birth.
• the 50 % survival cut-off is marked with an extended horizontal black arrow.
• the week where this 50 % survival cut off is reached is marked with a vertical arrow shown the same symbols as the associated life span curves (square, triangle, and diamond, see Figure 5A, figure legend).
• Regarding average survival per week from week 9: Compound 1 vs. saline p ⁇ 0.01; Compound 1 vs. DMSO p ⁇ 0.05; DMSO vs. saline n.s. (n 8 to 9).
• FIG. 5B shows that in comparison to Compound 1, prior art Compound A is unexpectedly significantly less efficacious at extending the lifespan of R6-2 mice.
• R6-2 mice treated with Compound A are indistinguishable from vehicle treated mice as well as saline treated mice.
• R6-2 mice treated with the classical TSPO ligand PK 11195 are indistinguishable from vehicle as well as saline treated mice, also regarding their lifespan (data not shown).
• Example 6 Superior Locomotor Improving Effect of Compound 1 in R6-2 mice, a transgenic mouse model for Huntington disease
• Compound 1 increases locomotion of R6-2 mice. As measured in an open field compared to untreated and vehicle treated mice of the transgenic R6-2 model for the Huntington disease, Compound 1 significantly enhances locomotor activity of R6-2 mice compared to their vehicle controls. Compound 1 also enhanced locomotor activity compared to sham (saline) control. In particular, from week 9, when the first mice death occurred, until week 16, when all mice were dead, average distance covered in an open field, per session, per experimental group, per week was measured in cm. No adverse effects due to Compound 1 application are observed. Compound 1 vs. DMSO p ⁇ 0.01; Compound 1 vs. saline trend (not shown). In comparison, prior art Compound A and PK 11195 did not show any locomotor improving effects (data not shown)
• Example 7 Cpd 1 is able to transform PC- 12 cells in cell culture to neuron like cells displaying extended neurites that define such neuronal differentiation (Figure 10). Briefly, PC- 12 cells were exposed to Cpd 1, in combination with glutamate, basically as described for the cell cultures of Example 1, and remained under that condition for 5 days. This resulted in neuron like cells with axon like protrusions ( Figure 10).
• Example 8 Superior Lifespan Extending Effects of Compounds 5 and 6 in R6-2 mice, a transgenic mouse model for Huntington disease
• mice were used as previously described in Example 5.
• Figures 12 and 13 show that Compounds 6 and 5 (respectively) increase the average lifespan of transgenic R6-2 mice, compared to the vehicle (DMSO) treated mice.
• the compounds of the invention may be prepared by the process described in general Scheme 1 hereinabove, and in Scheme 2 below. Briefly, to a suspension of (6.75 mmol) potassium hydride under argon in 30 ml of 1 ,2-dimethoxyethane (DME) is added the alcohol (4.5 mmol) in one portion. The reaction mixture is stirred for 30 minutes at room temperature and carbamoyl chloride (7 mmol) is added to the reaction mixture. Reaction mixture is brought to reflux for 2-5 hrs till completion of reaction (TLC monitored). The reaction is quenched with 30 ml of water carefully, followed by extraction with dichloromethane (or ethyl acetate).
• DME 1 ,2-dimethoxyethane
• CDCI 3 ⁇ 1.02-1.55 (m, 6H), 3.23-3.76 (m, 4H), 7.26-7.40 (m, 2H), 7.41-7.53 (m, 1H),

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