WO2015037720A1 - Promoteur de la réparation musculaire - Google Patents
Promoteur de la réparation musculaire Download PDFInfo
- Publication number
- WO2015037720A1 WO2015037720A1 PCT/JP2014/074291 JP2014074291W WO2015037720A1 WO 2015037720 A1 WO2015037720 A1 WO 2015037720A1 JP 2014074291 W JP2014074291 W JP 2014074291W WO 2015037720 A1 WO2015037720 A1 WO 2015037720A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein
- milk
- muscle synthesis
- casein
- whey
- Prior art date
Links
- 210000003205 muscle Anatomy 0.000 title claims abstract description 138
- 230000008439 repair process Effects 0.000 title abstract description 6
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 110
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 110
- 235000021119 whey protein Nutrition 0.000 claims abstract description 105
- 235000018102 proteins Nutrition 0.000 claims abstract description 101
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 101
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 101
- 102000011632 Caseins Human genes 0.000 claims abstract description 87
- 108010076119 Caseins Proteins 0.000 claims abstract description 87
- 239000000203 mixture Substances 0.000 claims abstract description 73
- 230000001737 promoting effect Effects 0.000 claims abstract description 54
- 238000010521 absorption reaction Methods 0.000 claims abstract description 45
- 230000015572 biosynthetic process Effects 0.000 claims description 129
- 238000003786 synthesis reaction Methods 0.000 claims description 129
- 102000014171 Milk Proteins Human genes 0.000 claims description 80
- 108010011756 Milk Proteins Proteins 0.000 claims description 80
- 235000021239 milk protein Nutrition 0.000 claims description 80
- 235000013336 milk Nutrition 0.000 claims description 60
- 239000008267 milk Substances 0.000 claims description 60
- 210000004080 milk Anatomy 0.000 claims description 60
- 235000013305 food Nutrition 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 34
- 230000000694 effects Effects 0.000 claims description 26
- 235000020124 milk-based beverage Nutrition 0.000 claims description 24
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 238000004806 packaging method and process Methods 0.000 claims description 20
- 230000037396 body weight Effects 0.000 claims description 18
- 235000020185 raw untreated milk Nutrition 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 14
- 235000012054 meals Nutrition 0.000 claims description 13
- 235000021243 milk fat Nutrition 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 13
- 235000008476 powdered milk Nutrition 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 238000012549 training Methods 0.000 claims description 9
- 235000020200 pasteurised milk Nutrition 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000005018 casein Substances 0.000 claims description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000021240 caseins Nutrition 0.000 claims description 2
- 235000019543 dairy drink Nutrition 0.000 claims 1
- 235000021075 protein intake Nutrition 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 6
- 235000013361 beverage Nutrition 0.000 description 22
- 238000012360 testing method Methods 0.000 description 22
- 239000000047 product Substances 0.000 description 20
- 241000700159 Rattus Species 0.000 description 18
- 230000009182 swimming Effects 0.000 description 18
- 239000000843 powder Substances 0.000 description 16
- 229920005560 fluorosilicone rubber Polymers 0.000 description 14
- 101150100968 fsr gene Proteins 0.000 description 14
- 238000010438 heat treatment Methods 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 210000002027 skeletal muscle Anatomy 0.000 description 12
- 239000007787 solid Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 9
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 8
- 239000012141 concentrate Substances 0.000 description 7
- 230000036541 health Effects 0.000 description 7
- 238000002347 injection Methods 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 6
- 229940024606 amino acid Drugs 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 230000037406 food intake Effects 0.000 description 6
- 239000008101 lactose Substances 0.000 description 6
- BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 5
- 102000008934 Muscle Proteins Human genes 0.000 description 5
- 108010074084 Muscle Proteins Proteins 0.000 description 5
- JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 5
- 239000005862 Whey Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 235000020183 skimmed milk Nutrition 0.000 description 5
- 239000003643 water by type Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 230000037213 diet Effects 0.000 description 4
- 235000015872 dietary supplement Nutrition 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000013861 fat-free Nutrition 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 235000013351 cheese Nutrition 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 235000013365 dairy product Nutrition 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000003505 heat denaturation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000008194 pharmaceutical composition Substances 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- XXMFJKNOJSDQBM-UHFFFAOYSA-N 2,2,2-trifluoroacetic acid;hydrate Chemical compound [OH3+].[O-]C(=O)C(F)(F)F XXMFJKNOJSDQBM-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 2
- 239000004278 EU approved seasoning Substances 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 238000007696 Kjeldahl method Methods 0.000 description 2
- 238000010162 Tukey test Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 210000000476 body water Anatomy 0.000 description 2
- 235000014121 butter Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 235000015140 cultured milk Nutrition 0.000 description 2
- 229910052805 deuterium Inorganic materials 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 235000011194 food seasoning agent Nutrition 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 235000015243 ice cream Nutrition 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 235000020121 low-fat milk Nutrition 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000014593 oils and fats Nutrition 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 235000011837 pasties Nutrition 0.000 description 2
- 239000000825 pharmaceutical preparation Substances 0.000 description 2
- 229940127557 pharmaceutical product Drugs 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000003405 preventing effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 235000015067 sauces Nutrition 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 2
- 210000001364 upper extremity Anatomy 0.000 description 2
- 235000021260 warm beverage Nutrition 0.000 description 2
- 235000021269 warm food Nutrition 0.000 description 2
- 235000013618 yogurt Nutrition 0.000 description 2
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000725101 Clea Species 0.000 description 1
- 241001137251 Corvidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 206010065713 Gastric Fistula Diseases 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 102000010445 Lactoferrin Human genes 0.000 description 1
- 108010063045 Lactoferrin Proteins 0.000 description 1
- 102000008192 Lactoglobulins Human genes 0.000 description 1
- 108010060630 Lactoglobulins Proteins 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000294411 Mirabilis expansa Species 0.000 description 1
- 235000015429 Mirabilis expansa Nutrition 0.000 description 1
- 206010049565 Muscle fatigue Diseases 0.000 description 1
- 208000029549 Muscle injury Diseases 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 238000010793 Steam injection (oil industry) Methods 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 235000012813 breadcrumbs Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000012839 cake mixes Nutrition 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- 108010033929 calcium caseinate Proteins 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 235000021557 concentrated beverage Nutrition 0.000 description 1
- 235000020186 condensed milk Nutrition 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 235000012495 crackers Nutrition 0.000 description 1
- 235000021438 curry Nutrition 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 235000021185 dessert Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 235000015071 dressings Nutrition 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- -1 etc.) Substances 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000004503 fine granule Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000013611 frozen food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000008446 instant noodles Nutrition 0.000 description 1
- 235000014109 instant soup Nutrition 0.000 description 1
- 229960002725 isoflurane Drugs 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
- 235000021242 lactoferrin Nutrition 0.000 description 1
- 229940078795 lactoferrin Drugs 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 235000020191 long-life milk Nutrition 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 235000013310 margarine Nutrition 0.000 description 1
- 239000003264 margarine Substances 0.000 description 1
- 239000008268 mayonnaise Substances 0.000 description 1
- 235000010746 mayonnaise Nutrition 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 230000037257 muscle growth Effects 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 235000015108 pies Nutrition 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 235000013324 preserved food Nutrition 0.000 description 1
- 235000014059 processed cheese Nutrition 0.000 description 1
- 230000004845 protein aggregation Effects 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 208000001076 sarcopenia Diseases 0.000 description 1
- 210000002363 skeletal muscle cell Anatomy 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 229940080237 sodium caseinate Drugs 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 235000021055 solid food Nutrition 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 235000013322 soy milk Nutrition 0.000 description 1
- 235000011496 sports drink Nutrition 0.000 description 1
- 235000014268 sports nutrition Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 235000013547 stew Nutrition 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
- 235000021249 α-casein Nutrition 0.000 description 1
- 235000021241 α-lactalbumin Nutrition 0.000 description 1
- 235000021247 β-casein Nutrition 0.000 description 1
- 235000021246 κ-casein Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/15—Reconstituted or recombined milk products containing neither non-milk fat nor non-milk proteins
- A23C9/1512—Reconstituted or recombined milk products containing neither non-milk fat nor non-milk proteins containing isolated milk or whey proteins, caseinates or cheese; Enrichment of milk products with milk proteins in isolated or concentrated form, e.g. ultrafiltration retentate
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/06—Anabolic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/20—Milk; Whey; Colostrum
Definitions
- the present invention relates to muscle synthesis promoters for subjects in need of immediate protein absorption.
- Whey protein has a high rate (absorption time) from ingestion to digestion and absorption (short time) and contains a lot of BCAA (branched chain amino acids: valine, leucine, isoleucine), thus preventing muscle fatigue, It is said that muscles can be strengthened efficiently.
- BCAA branched chain amino acids: valine, leucine, isoleucine
- casein protein is slowly and continuously metabolized compared to whey protein, so the rate from oral ingestion to digestion absorption (absorption rate) is slow (longer time) and needs immediate absorption. Is said to be unsuitable (see Patent Document 1). Therefore, whey protein is mainly used as protein powder.
- a mixed protein in which whey protein having high absorption and casein protein to be continuously absorbed are mixed at 1: 1 has been developed (for example, DNS protein slow loading). ).
- whey protein has low heat resistance (thermal stability), it undergoes thermal denaturation and tends to aggregate due to heating.
- protein powders are not recommended for consumption other than being dissolved in water. Therefore, protein powder remains powdery and cannot be said to have high palatability.
- the whey protein is hardened by heat denaturation and there is a problem that the absorption efficiency is lowered.
- an object of the present invention is to provide a muscle synthesis (enhancement) promoter having an immediate effect of promoting muscle synthesis equivalent to that of whey protein.
- a muscle synthesis promoter for a subject in need of immediate protein absorption comprising a milk protein mixture containing casein protein and whey protein as an active ingredient, and the amount of casein protein relative to whey protein
- a muscle synthesis promoter having a ratio (casein protein / whey protein) of 2 to 20.
- the muscle synthesis promoter described in 1. (4) The muscle synthesis promoter according to any one of (1) to (3) above, wherein the subject in need of immediate protein absorption is a subject to exercise. (5) The muscle synthesis promoter according to (4) above, wherein the exercise is muscle training. (6) The muscle synthesis promoter according to any one of (1) to (5), which is in a liquid form.
- a packaged container-packed milk drink that is provided with an action for promoting muscle synthesis by immediate protein absorption.
- a method for producing a packaged-packed milk beverage comprising a step of adding to one or more selected from the group consisting of milk and a step of heat sterilization, wherein the milk protein mixture is 8 g /
- a milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is taken by a subject who needs immediate absorption of the protein
- a method of promoting muscle synthesis comprising: (11) A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is taken by a subject who needs immediate absorption of the protein.
- a method for promoting muscle synthesis comprising: (12) The method for promoting muscle synthesis according to (10) or (11) above, wherein the milk protein mixture is ingested so that the total protein amount is 0.13 to 0.7 g / kg body weight per serving. (13) The method for promoting muscle synthesis according to (12), wherein the milk protein mixture is ingested so that the total protein amount of the milk protein mixture is 8 to 40 g per serving. (14) The method for promoting muscle synthesis according to any one of the above (10) to (13), comprising ingesting the milk protein mixture in a heated liquid form to a subject in need of immediate absorption of the protein.
- the muscle synthesis promoter of the present invention has an immediate effect of promoting muscle synthesis equivalent to that of whey protein, and has higher heat resistance and better processability than using whey protein alone. Therefore, the muscle synthesis promoter of the present invention is advantageous in that it can be expected to have an immediate effect of promoting muscle synthesis and can be provided as an inexpensive product form that can be mass-produced commercially.
- FIG. 1 shows the skeletal muscle synthesis rate (FSR) in the triceps surae muscle and the hyfuku muscle when orally ingesting (administering) milk protein solutions of various concentrations.
- FIG. 2 shows changes in FSR with swimming exercise.
- FIG. 3 shows the blood 3MeHis concentration when each concentration of milk protein solution was orally ingested (administered).
- the muscle synthesis promoter of the present invention is a muscle synthesis promoter for a subject in need of immediate protein absorption, comprising a milk protein mixture containing casein protein and whey protein as an active ingredient. It is a muscle synthesis promoter having a protein amount ratio (casein protein / whey protein) of 2 to 20 to the casein protein.
- the active ingredient means an ingredient required for exerting an effect of promoting muscle synthesis for a subject requiring immediate absorption of protein.
- Subjects requiring immediate absorption of protein means subjects who need to promote muscle protein synthesis in order to repair muscle tissue and promote muscle growth, such as health promotion and exercise capacity
- the exercise is preferably strength training (resistance exercise), more preferably long-term strength training, for example, continuous, in that muscle synthesis is promoted by applying a certain load to the strength.
- the strength training is continued for 30 minutes to 5 hours at a frequency of 1 to 7 days in a week.
- Examples of strength training include dumbbell exercise, squat exercise, and machine training.
- Examples of subjects who take the muscle synthesis promoter of the present invention include, for example, athletes, sports enthusiasts (athletes), those who need exercise to improve lifestyle-related diseases, and elderly people for health promotion. Examples include those who require exercise, or patients who suffer from a muscle injury disease (eg, sarcopenia) and who need to promote muscle synthesis through exercise. Further, the target may be an animal other than a human (domestic animals such as horses and cows, pets such as dogs and cats, appreciation animals kept in zoos, etc.).
- the time when the muscle synthesis promoter of the present invention is ingested (administered) to a subject in need of immediate protein absorption may be before exercise, after exercise, or during exercise.
- the time of intake is preferably after exercise, more preferably immediately after exercise.
- the intake period is preferably within 3 hours after the end of exercise, immediately after exercise. More preferably, within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, and particularly preferably within 30 minutes after the end of exercise (Levenhagenhaet al. Am J physiol Endocrinol Metab (2001), 280, E982-E993).
- Immediately effective muscle synthesis promoting effect equivalent to whey protein is the same level of protein absorption rate and absorption as observed when orally ingesting (administering) a protein mainly composed of whey protein. It means the same level of action as the accompanying muscle synthesis promoting action.
- the muscle synthesis promoting action includes an anabolic action of muscle protein from amino acids.
- the muscle synthesis promoting action can be confirmed, for example, by measuring the triceps surae muscle synthesis rate (FSR) of the upper limb in the test group within 1 hour after administration of the test substance after the rat swimming test. it can.
- FSR triceps surae muscle synthesis rate
- the muscle synthesis promoting action includes a muscle protein degradation inhibiting action, that is, a catabolic preventing action from muscle protein to amino acid.
- the degradation inhibitory action of muscle protein can be confirmed by measuring the concentration of 3 methylhistidine within 1 hour after administration of the test substance after, for example, a rat swimming test (Example 1). (See (3)).
- Protein amount means the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein or casein protein.
- Protein amount ratio means the ratio of the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein, or casein protein.
- the amount of protein may be calculated based on known information such as a food composition table, or may be calculated by measuring by a conventional method such as Kjeldahl method or Raleigh method. Actually, in the case of the Kjeldahl method, it can be calculated by measuring nitrogen contained in various proteins and multiplying the value by a nitrogen-protein conversion factor (usually 6.25).
- the milk protein mixture used in the present invention is not particularly limited as long as it contains whey protein and casein protein in a specific protein amount ratio, but the whey protein and casein protein separated and purified separately are not specifically limited. It may be prepared by mixing so as to obtain a protein amount ratio, or a raw material (material) or food and drink that already contains whey protein and casein protein that have not been separated or purified separately in a specific protein amount ratio You may mix and prepare goods. At this time, the milk protein mixture used in the present invention does not require a separation step or purification step of whey protein or casein protein, and the production cost thereof is low, so that whey protein and casein protein are already specified. Prepared by mixing the raw materials and foods and drinks that are contained in the protein content ratio as the main components, and mixing the whey protein and casein protein purified separately as needed to achieve a specific protein content ratio. Is preferred.
- whey protein separated and purified separately from casein protein examples include whey stock solutions (sweet whey, acid whey, etc.), concentrates, dried products (whey powder, etc.), frozen products, hydrolyzed products, etc. And reducing solutions thereof. Further, desalted whey, whey protein concentrate (WPC), purified whey protein (WPI), ⁇ -lactalbumin ( ⁇ -La), ⁇ -lactoglobulin ( ⁇ -Lg), immunoglobulin, lactoferrin, and the like, and These reducing solutions can be used.
- Casein protein separated and purified separately from whey protein includes, for example, casein isolate (natural cheese such as fresh cheese, sodium caseinate, calcium caseinate), its concentrate, its dried product, its A frozen material, a hydrolyzate thereof, a reducing solution thereof, and the like can be used. Furthermore, purified products such as ⁇ -casein, ⁇ -casein, and ⁇ -casein, and reducing solutions thereof can be used.
- raw materials and foods and beverages that already contain whey protein and casein protein in a specific protein ratio include, for example, raw milk, pasteurized milk (such as milk), skim milk, component-adjusted milk, processed milk, and dairy products ( Concentrated milk, powdered milk, condensed milk, fermented milk (yogurt, etc.), lactic acid bacteria beverages, processed cheeses, ice creams, creams), milk protein concentrate (MPC), its concentrate, its dried product, its frozen product, its Hydrolysates and the like, and reducing solutions thereof can be used.
- MPC milk protein concentrate
- the milk protein mixture used in the present invention is composed of whey protein and casein protein having a specific protein amount ratio.
- the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, preferably 2 to 10, more preferably 2 to 6, still more preferably 2 to 4, and particularly preferably 2 to 3.5.
- the protein ratio (casein protein / whey protein) between casein protein and whey protein is intentionally adjusted (designed), which is greatly different from the conventional technology. Yes.
- the muscle synthesis promoter of the present invention has high heat resistance (thermal stability) and exhibits a heat resistance effect.
- having a heat-resistant effect means that the protein does not solidify (aggregate) due to heat denaturation even when it is treated at a specific temperature. Specifically, it is a condition used in general processing of milk. This means that the protein is not heat denatured even if heat-sterilized by the method.
- the muscle synthesis promoter of the present invention has a heat resistance effect. More specifically, it is heat sterilized (LTLT treatment: low temperature and long time heating) at 62 to 65 ° C.
- the heat sterilization method includes a batch type in which the processing liquid is heated and held using a tank or the like, an indirect heating type using a plate type heat exchanger or a tube type heat exchanger, and a steam injection type heating device. And a known method such as a direct heating method using a steam infusion type heating device or a current heating method using a Joule heating device can be used.
- the milk protein mixture is ingested (administered) so that the total protein amount is 0.13 g / kg body weight or more per meal, Specifically, the weight is 0.13 to 0.7 g / kg body weight, preferably 0.13 to 0.6 g / kg body weight, more preferably 0.13 to 0.5 g / kg body weight, still more preferably 0.00. It is ingested (administered) so as to be 15 to 0.4 g / kg body weight, particularly preferably 0.2 to 0.4 g / kg body weight.
- the typical weight of the subject is estimated to be about 60 kg.
- one meal means one time in a state requiring immediate absorption of protein.
- a single meal is not taken at a time (at a time), but several times. May be taken separately.
- the amino acid concentration in the bloodstream can be increased immediately when it is ingested in several degrees. In this respect, it is preferably within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, more preferably within 30 minutes after the end of exercise, and particularly preferably the end of exercise. Within 5 minutes later.
- the method for ingesting (administering) the muscle synthesis promoter of the present invention to a subject in need of immediate protein absorption can be appropriately selected from oral ingestion (oral administration), enteral administration, gastric fistula and the like depending on the subject and application. Although it can be selected, it is preferably taken orally.
- the muscle synthesis promoter of the present invention can take any form as long as the effect is exerted, and examples thereof include a solid form, a liquid form, a gel form, a paste form, and a paste form.
- a solid form among solids, it may be powder, granule, capsule, block, In view of good processability, it is preferably a liquid.
- a long-term storage period can be set in a refrigerated state and at room temperature.
- the water content can be appropriately adjusted. Specifically, it may be 10 to 95% by mass, preferably 20 to 95% by mass, and more preferably. Is 30 to 90% by mass, more preferably 40 to 90% by mass.
- the water content or solid content
- muscle synthesis can be effectively promoted.
- the muscle synthesis promoter of the present invention comprises a unit package form per serving, and the milk protein mixture is added to the total amount of food intake in the unit package form. It is desirable that the amount of protein is adjusted to 8 g or more, specifically, 8 to 40 g, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably 10 to 15 g. It adjusts so that it may become.
- ⁇ consisting of a unit packaging form per meal '' is a form in which the intake amount per meal is determined in advance, for example, as a food and drink that can be taken orally in a specific amount, not only general foods, It means forms such as beverages (drinks, etc.), health supplements, health functional foods, and supplements.
- unit packaging form per meal for example, in the case of liquid beverages, gel-like, paste-like, paste-like jelly, powder-like, granule-like, capsule-like, block-like solid foods, etc.
- a specific amount (dose) can be defined in packaging containers such as cans, glass bottles (such as bottles), plastic containers (such as plastic bottles), packs, pouches, film containers, paper boxes, etc., or intake per serving (usage, dose) ) Is displayed on a packaging container, a homepage, etc., and the form which can prescribe
- packaging containers such as cans, glass bottles (such as bottles), plastic containers (such as plastic bottles), packs, pouches, film containers, paper boxes, etc., or intake per serving (usage, dose) ) Is displayed on a packaging container, a homepage, etc., and the form which can prescribe
- the form of the muscle synthesis promoter of the present invention is a so-called health drink
- 8 to 40 g of the beverage in which the milk protein is suspended or dissolved is provided.
- the capacity (dose) of a single drink per meal is adjusted by the amount of water so that it is 100 to 1000 ml, and is placed in a packaging container such as a metal can, a glass bottle (such as a bottle), or a plastic container (such as a plastic bottle). (Filled) form.
- 8 to 40 g of the above-described milk protein suspension or dissolved beverage is displayed on a packaging container or homepage as the amount of intake (usage, dose) (for example, 100 to 1000 ml) per serving, and several servings (several (Batch) is a collective capacity, and is a form that is put (filled) in a large-capacity packaging container or the like.
- the muscle synthesis promoter of the present invention is manufactured so as to contain a predetermined milk protein mixture
- its manufacturing process is not limited.
- the muscle synthesis promoter of the present invention includes various components such as milk protein and milk fat with respect to raw milk (raw milk), sterilized milk, concentrated milk, powdered milk, and reduced milk according to a known manufacturing method (manufacturing method). It can be manufactured by blending (adjusting) the ingredients, or various ingredients such as milk protein and milk fat can be blended (adjusted) with component-adjusted milk and processed milk (such as low-fat milk and non-fat milk). ) Can also be manufactured.
- the muscle synthesis promoter of the present invention can provide a composition comprising a muscle synthesis promoter. That is, the muscle synthesis promoter of the present invention can be used alone as it is, but as long as the muscle synthesis promotion function is exhibited, various compositions for oral intake (for oral administration) such as foods and drinks and pharmaceuticals. A composition (for example, a pharmaceutical composition or a food / beverage product composition) having an immediate effect of promoting muscle synthesis can also be obtained. Therefore, according to one embodiment of the present invention, a composition comprising the muscle synthesis promoter of the present invention is provided.
- Food and drink is something other than a pharmaceutical product (pharmaceutical composition), as long as it can be taken orally, such as a solution, suspension, emulsion, powder, solid molded product, etc.
- a pharmaceutical product pharmaceutical composition
- Well not particularly limited.
- food and drink include instant noodles, retort food, canned food, microwave food, instant soup and miso soup, freeze-dried food, etc .; soft drinks, fruit juice drinks, vegetable drinks, soy milk drinks, coffee Beverages such as beverages, tea beverages, powdered beverages, concentrated beverages, alcoholic beverages; flour products such as bread, pasta, noodles, cake mixes, bread crumbs; strawberries, caramel, chewing gum, chocolate, cookies, biscuits, cakes, pies, snacks , Crackers, Japanese confectionery, dessert confectionery, etc .; sauces, tomato processing seasonings, flavor seasonings, cooking mixes, sauces, dressings, soups, curry and stew ingredients; processed oils and fats, butter Oils and fats such as margarine and mayonnaise; milk drinks, fermented milk (yogurt, etc.), lactic acid bacteria drinks, natural tea 'S, process cheese, ice cream, dairy products such as cream; agricultural canning, jam, marmalade such, processed agricultural products such as serial; such as frozen food, and the like.
- Foods and drinks include health foods, functional foods, dietary supplements, foods for specified health use, foods for the sick, infant formula, powdered milk for pregnant or lactating women, or rapid muscle synthesis.
- subjected the indication that it is a thing used for promotion is also included.
- “Pharmaceuticals” are those prepared as oral preparations or parenteral preparations in accordance with conventional methods, using additives that are acceptable for formulation.
- the pharmaceutical is an oral preparation, it should be in the form of a solid preparation such as a tablet, powder, fine granule, granule, capsule, pill or sustained release, or a liquid preparation such as a solution, suspension or emulsion. Can take.
- a pharmaceutical is a parenteral formulation, it can take the form of an injection or a suppository. In terms of ease of ingestion (administration) to a patient, the pharmaceutical is preferably an oral preparation.
- additives that are acceptable for formulation include, for example, excipients, stabilizers, preservatives, wetting agents, emulsifiers, lubricants, sweeteners, coloring agents, fragrances, buffers, antioxidants. Agents, pH adjusters and the like.
- the muscle synthesis promoter of the present invention has a heat resistance effect. Therefore, in the composition in which the muscle synthesis promoter of the present invention is blended (added, mixed), the muscle synthesis promoter of the present invention has a heat-resistant effect, so that it can be preferably provided as a liquid product form. It can be provided as a product form for warm sales using a hot vendor or the like.
- composition of the muscle synthesis promoter of the present invention may be in any form as long as the effect is exerted, for example, solid, liquid, gel, Examples include pasty and pasty forms.
- the composition containing the muscle synthesis promoter of the present invention may be in the form of a solid, powder, granule, capsule, or block. However, it is preferably a liquid in terms of good processability.
- the present invention is a food / beverage product obtained by blending (adding, mixing) and heat-sterilizing the muscle synthesis promoter of the present invention, comprising a unit packaging form per serving, and the unit packaging form Among them, it is desirable that the milk protein mixture is adjusted to a total protein amount of 8 to 40 g as a single meal intake, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably.
- Food / beverage products and methods for producing the same are provided, which are provided with an effect of promoting muscle synthesis by immediate absorption of protein, adjusted to 10 to 15 g.
- the operation (process) of “mixing (addition, mixing) and heat sterilization” to a predetermined component is not particularly limited as long as the operation is finally adjusted to a predetermined component and sterilized. Operation that mixes all necessary components and adjusts them to the prescribed components and then heat sterilizes them together, or operation that sterilizes the necessary individual components separately and mixes them to adjust them to the prescribed components Etc. Therefore, in the present invention, the order and number of times of this blending (addition, mixing) and heat sterilization are arbitrary.
- the food / beverage product (the food / beverage product of the present invention) is preferably a food / beverage product packed in a packaging container, and more preferably a food / beverage product packed in a packaging container for heat sale. That is, since the food and drink have heat resistance, the protein will not be extremely denatured even if it is heated and sold. Therefore, in the food and drink composed only of the conventional whey protein or the whey protein as a main component, as a warm food and drink, it could not be provided outdoors in winter, etc., whereas in the food and drink, as a warm food and drink, It can be provided outdoors in winter (especially in cold conditions) and can be consumed (drinked) by consumers.
- the practical use of the food-drinks with the effect of accelerating muscle synthesis corresponding to new needs can be expected.
- a hot sale beverage or a hot sale food stored in a hot vendor or hot water for example, a hot sale beverage or a hot sale food stored in a hot vendor or hot water, and a necessary amount are put in a cooking utensil.
- heated beverages and heated foods heated by gas stoves, electric heating stoves, microwaves, and the like can be mentioned.
- warm sales it is generally stored at around 30-80 ° C or around 50-80 ° C.
- the food or drink is a milk drink.
- milk beverage means a beverage containing milk solids (a combination of nonfat milk solids and milk fat) at 3.0% by weight or more.
- vitamins, minerals, oligosaccharides, lactose, fruit juice and the like can be blended in the milk beverage.
- the production process is not limited as long as the food or drink is produced so as to contain a predetermined milk protein mixture (at a predetermined concentration). It can manufacture based on manufacturing processes, such as adjusted milk, processed milk, and a milk drink.
- the food / beverage products of this invention mix
- a packaged container-packed milk beverage that is preferably adjusted to 0.5 to 4 g / 200 ml, particularly preferably 0.5 to 3
- a milk protein mixture comprising casein protein and whey protein, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20,
- a method for producing a packaged-packed milk beverage comprising a step of adding to one or more selected from the group consisting of raw milk, pasteurized milk, concentrated milk, powdered milk and reduced milk, and a heat sterilization step, wherein the milk protein
- the total protein amount of the mixture is adjusted to 8 g / 200 ml, preferably 8 to 20 g / 200 ml, more preferably 9 to 20 g / 200 ml, still more preferably 9 to 15 g / 200 ml, particularly preferably 10 to 15 g / 200 ml
- Milk fat content is 8g or less / 200ml, preferably 0.1-8g 200 ml, more preferably 0.1 to 6 g / 200 ml, further preferably 0.5 to 4 g / 200 ml,
- “Reduced milk” means liquid milk prepared by dispersing and dissolving milk raw materials such as powdered milk and concentrated milk in water.
- milk raw material include whole milk powder, skim milk powder, whole fat concentrated milk, nonfat concentrated milk, butter, cream, and unfinished milk.
- aggregation due to heat denaturation of the whey protein is not observed (see Example 2), and therefore, the immediate protein absorption effect is maintained as in the muscle synthesis promoter of the present invention.
- a milk protein mixture comprising casein protein and whey protein, wherein the protein content ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20,
- a method of promoting muscle synthesis comprising ingesting a subject in need of absorption.
- a milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is instantly absorbed by the protein.
- a method for promoting muscle synthesis comprising ingesting a subject in need thereof.
- the “medical action for humans” means an action that requires a doctor or the like to ingest (administer) a medicine to a human.
- the muscle synthesis promoting method of the present invention can be carried out according to the contents described in the present specification for the muscle synthesis promoting agent of the present invention.
- the milk protein mixture is ingested (administered) so that the total protein amount is 0.13 g / kg body weight or more per meal.
- the weight is 0.13-0.7 g / kg body weight, preferably 0.13-0.6 g / kg body weight, more preferably 0.13-0.5 g / kg body weight, still more preferably It can be ingested so as to be 0.15 to 0.4 g / kg body weight, particularly preferably 0.2 to 0.4 g / kg body weight.
- the milk protein mixture in the method for promoting muscle synthesis of the present invention, can be ingested so that the total protein amount is 8 g or more per serving. It can be ingested so as to be ⁇ 40 g, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably 10 to 15 g.
- the milk protein mixture in the muscle synthesis promotion method of the present invention, can be ingested in a heated liquid form to a subject that needs immediate absorption of the protein.
- a muscle synthesis promoting agent comprising a milk protein mixture comprising casein protein and whey protein for promoting muscle synthesis of a subject in need of immediate protein absorption.
- a muscle synthesis promoter having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is provided.
- the use of the muscle synthesis promoter of the present invention can be carried out according to the contents described herein for the muscle synthesis promoter of the present invention.
- a milk protein mixture comprising casein protein and whey protein for the manufacture of a food or beverage or pharmaceutical product for promoting muscle synthesis in a subject in need of immediate protein absorption.
- a muscle synthesis promoter comprising a muscle synthesis promoter having a protein content ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is provided.
- the use of the muscle synthesis promoter of the present invention can be carried out according to the contents described herein for the muscle synthesis promoter of the present invention.
- muscle synthesis promoting effect muscle synthesis promoting effect
- body water retention action body water retention effect
- Example 1 Muscle synthesis promoting action by milk protein mixture
- Rat swimming test SD male rats obtained from CLEA Japan, Inc. having an initial weight of 140 to 160 g were used as experimental animals. Rats were divided into 6 groups of 9 to 10 animals so that there was no difference in body weight between test groups (groups). All rats were individually housed in stainless steel cages at room temperature of 22 ⁇ 2 ° C. with a light period of 7 to 19 o'clock and a dark period of 19 to 7 o'clock.
- rats were preliminarily raised for 3 to 4 days, and the rats were subjected to a swimming test according to the following protocol (the rats were exercised).
- Rat swimming test (exercise) protocol Day 1: Rats were subjected to 2 hours of swimming exercise, 45 minutes of rest, 2 hours of swimming exercise for a total of 4 hours of swimming without weight load. The diet after exercise (solid feed MF) was freely taken. Day 2: The rats were subjected to 3 hours of swimming exercise, 45 minutes of rest, 3 hours of swimming exercise for a total of 6 hours without weight load. The diet after exercise (solid feed MF) was freely taken. Day 3: Rats were subjected to a total of 6 hours of swimming exercise without weight load, 3 hours of swimming exercise, 45 minutes of rest, 3 hours of swimming exercise. As a diet after exercise, 5 g of a restricted diet (powder feed MF) was ingested.
- a restricted diet porice feed MF
- the rats were subjected to a 2-hour swimming exercise without weight load.
- a milk beverage (milk protein solution) was prepared using milk protein and milliQ water so that the protein concentrations shown in Table 1 were obtained.
- the rats were orally ingested milk drink and milliQ water at 24.24 ml / kg BW (test group 1-5 and comparative group 1).
- the milk protein includes milk protein concentrate (MPC480, manufactured by Fontera, milk protein 77.2% by weight, milk fat content 1.2% by weight, lactose 9.2% by weight, ash content 7.1% by weight, Water (5.3 wt%) was used.
- This MPC480 is a mixture of casein protein and whey protein obtained by subjecting skim milk to membrane separation treatment (UF treatment, MF treatment, etc.), removing lactose and ash, and concentrating mainly on casein protein and whey protein.
- the form is a dry powder.
- apparatus LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: 0.05% TFA water / 0.05% TFA-containing acetonitrile, injection volume 3 ⁇ l, flow rate 0.3 ml / min, UV 215 nm.
- FSR skeletal muscle synthesis rate
- the obtained numerical values were statistically processed to obtain an average value ⁇ standard error. Specifically, a one-way analysis of variance was performed, followed by Tukey's multiple comparison test. The significance level was less than 5% (p ⁇ 0.05). The result is shown in FIG. When there was a significant difference in the figure, different characters were attached. For example, it is shown that there is a significant difference between a group with a (comparison group 1 or the like) and a group with b (test group 3) or a group with c (test group 4 or the like). ing. Further, for example, it is shown that there is no significant difference between a group to which a (comparison group 1 or the like) or b (test group 3) is attached and a group to which ab is attached (test group 2).
- the obtained numerical values were statistically processed to obtain an average value ⁇ standard error. Specifically, a one-way analysis of variance was performed, followed by Tukey's multiple comparison test. The significance level was less than 5%. The result is shown in FIG. When there was a significant difference in the figure, different characters were attached.
- Example 2 Muscle synthesis promoting action by milk beverage containing milk protein (1) Preparation (production) of milk beverage containing milk protein Raw milk (milk protein 3.3 wt%, milk fat 3.8 wt%, lactose 4.8 wt%, ash 0.7 wt%, moisture 87.4 wt%, obtained from Meiji Co., Ltd.) Protein was added and mixed to prepare raw milk so that the milk protein concentration shown in Table 2 was obtained. And according to the usual method for producing milk, the indirect heating method using a plate heat exchanger is sterilized by heating at 130 ° C. for 2 seconds, and then cooled to 10 ° C. or lower, so that the milk protein has a high concentration. (Milk test solution 6 and 7) was obtained.
- Raw milk milk protein 3.3 wt%, milk fat 3.8 wt%, lactose 4.8 wt%, ash 0.7 wt%, moisture 87.4 wt%, obtained from Meiji Co., Ltd.
- the milk protein added to the milk beverage includes milk protein concentrate (MPC480, manufactured by Fontera, milk protein 77.2% by weight, milk fat content 1.2% by weight, lactose 9.2% by weight, ash content 7 0.1 wt%, water 5.3 wt%).
- MPC480 is a mixture of casein protein and whey protein obtained by subjecting skim milk to membrane separation treatment (UF treatment, MF treatment, etc.), removing lactose and ash, and concentrating mainly on casein protein and whey protein.
- the form is a dry powder.
- FSR skeletal muscle synthesis rate
- test sections 6 and 7 were as high as 6.7% / day and 7.7% / day, respectively.
- the FSR is 8.8% / day when whey protein is taken orally at 3.11 g / kg BW after 2 hours of treadmill exercise as a protein amount. , J. Nutr. 137, 357-362 (2007)).
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Nutrition Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Mycology (AREA)
- Neurology (AREA)
- Epidemiology (AREA)
- Physical Education & Sports Medicine (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Zoology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Dairy Products (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
L'invention concerne un promoteur de la réparation musculaire destiné à être utilisé dans une cible dont l'absorption rapide des protéines est requise, et contenant comme ingrédient efficace un mélange de lactoprotéines qui inclut de la protéine de caséine et de la protéine de lactosérum, le promoteur de la réparation musculaire ayant un rapport protéique de la protéine de caséine à la protéine de lactosérum (protéine caséine/protéine lactosérum) de 2-20. Le promoteur de la réparation musculaire a une action favorisant la réparation musculaire rapide équivalente à celle de la protéine de lactosérum.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG11201601774VA SG11201601774VA (en) | 2013-09-12 | 2014-09-12 | Muscle synthesis promoter |
JP2015536652A JPWO2015037720A1 (ja) | 2013-09-12 | 2014-09-12 | 筋肉合成促進剤 |
CN201480050083.3A CN105658231A (zh) | 2013-09-12 | 2014-09-12 | 肌肉合成促进剂 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2013-189703 | 2013-09-12 | ||
JP2013189703 | 2013-09-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2015037720A1 true WO2015037720A1 (fr) | 2015-03-19 |
Family
ID=52665816
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2014/074291 WO2015037720A1 (fr) | 2013-09-12 | 2014-09-12 | Promoteur de la réparation musculaire |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPWO2015037720A1 (fr) |
CN (1) | CN105658231A (fr) |
SG (1) | SG11201601774VA (fr) |
WO (1) | WO2015037720A1 (fr) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016121923A1 (fr) * | 2015-01-29 | 2016-08-04 | 株式会社明治 | Promoteur de synthèse musculaire |
WO2017026429A1 (fr) * | 2015-08-10 | 2017-02-16 | 株式会社明治 | Agent favorisant la synthèse du muscle |
WO2018164251A1 (fr) * | 2017-03-10 | 2018-09-13 | 株式会社明治 | Composition pour améliorer la résistance physique |
WO2019124355A1 (fr) * | 2017-12-18 | 2019-06-27 | 株式会社明治 | Lait fermenté activant la synthèse musculaire |
WO2020013307A1 (fr) * | 2018-07-13 | 2020-01-16 | 株式会社明治 | Procédé de production d'un yogourt concentré |
JP2020014452A (ja) * | 2018-07-13 | 2020-01-30 | 株式会社明治 | 濃縮発酵乳の製造方法 |
WO2020085409A1 (fr) * | 2018-10-25 | 2020-04-30 | 味の素株式会社 | Agent d'amélioration du corps |
US20210329949A1 (en) * | 2020-04-27 | 2021-10-28 | Leprino Performance Brands LLC | Protein-fortified beverages for enhanced athletic performance |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI761487B (zh) * | 2017-04-06 | 2022-04-21 | 日商明治股份有限公司 | 用於促進血中胺基酸濃度上升之發酵乳 |
CN114431380A (zh) * | 2022-02-25 | 2022-05-06 | 广东君悦营养医学有限公司 | 一种适用于肌肉衰减综合症的蛋白水及其制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012110705A1 (fr) * | 2011-02-18 | 2012-08-23 | Valio Ltd | Produit à base de lait et son procédé de préparation |
JP2013517808A (ja) * | 2010-01-29 | 2013-05-20 | アボット・ラボラトリーズ | カルシウムhmbを含む栄養エマルジョン |
JP2013517807A (ja) * | 2010-01-29 | 2013-05-20 | アボット・ラボラトリーズ | Hmbを含有するプラスチック包装栄養液剤 |
-
2014
- 2014-09-12 SG SG11201601774VA patent/SG11201601774VA/en unknown
- 2014-09-12 CN CN201480050083.3A patent/CN105658231A/zh active Pending
- 2014-09-12 WO PCT/JP2014/074291 patent/WO2015037720A1/fr active Application Filing
- 2014-09-12 JP JP2015536652A patent/JPWO2015037720A1/ja active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013517808A (ja) * | 2010-01-29 | 2013-05-20 | アボット・ラボラトリーズ | カルシウムhmbを含む栄養エマルジョン |
JP2013517807A (ja) * | 2010-01-29 | 2013-05-20 | アボット・ラボラトリーズ | Hmbを含有するプラスチック包装栄養液剤 |
WO2012110705A1 (fr) * | 2011-02-18 | 2012-08-23 | Valio Ltd | Produit à base de lait et son procédé de préparation |
Non-Patent Citations (2)
Title |
---|
DAICHI NAKAJIMA ET AL.: "Effect of total milk proteins ingestion on muscle mass and body fat", FOOD STYLE 21, vol. 16, no. 9, 2012, pages 76 - 78 * |
TAKAYUKI KITAGISHI ET AL.: "Intake of total milk proteins and prevention of sarcopenia", FOOD STYLE 21, vol. 17, no. 4, 1 April 2013 (2013-04-01), pages 56 - 58 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016121923A1 (fr) * | 2015-01-29 | 2016-08-04 | 株式会社明治 | Promoteur de synthèse musculaire |
WO2017026429A1 (fr) * | 2015-08-10 | 2017-02-16 | 株式会社明治 | Agent favorisant la synthèse du muscle |
JPWO2017026429A1 (ja) * | 2015-08-10 | 2018-05-31 | 株式会社明治 | 筋肉合成促進剤 |
WO2018164251A1 (fr) * | 2017-03-10 | 2018-09-13 | 株式会社明治 | Composition pour améliorer la résistance physique |
WO2019124355A1 (fr) * | 2017-12-18 | 2019-06-27 | 株式会社明治 | Lait fermenté activant la synthèse musculaire |
JPWO2019124355A1 (ja) * | 2017-12-18 | 2020-12-10 | 株式会社明治 | 筋肉合成促進用発酵乳 |
WO2020013307A1 (fr) * | 2018-07-13 | 2020-01-16 | 株式会社明治 | Procédé de production d'un yogourt concentré |
JP2020014452A (ja) * | 2018-07-13 | 2020-01-30 | 株式会社明治 | 濃縮発酵乳の製造方法 |
WO2020085409A1 (fr) * | 2018-10-25 | 2020-04-30 | 味の素株式会社 | Agent d'amélioration du corps |
US20210329949A1 (en) * | 2020-04-27 | 2021-10-28 | Leprino Performance Brands LLC | Protein-fortified beverages for enhanced athletic performance |
Also Published As
Publication number | Publication date |
---|---|
CN105658231A (zh) | 2016-06-08 |
JPWO2015037720A1 (ja) | 2017-03-02 |
SG11201601774VA (en) | 2016-04-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2015037720A1 (fr) | Promoteur de la réparation musculaire | |
JP6728071B2 (ja) | 筋肉合成促進剤 | |
WO2006017374A2 (fr) | Composition de supplement nutritionnel et procede correspondant | |
JP7108521B2 (ja) | 体内bdnf量増加促進用組成物 | |
US20050112177A1 (en) | Nutritional supplement composition and method | |
WO2017026429A1 (fr) | Agent favorisant la synthèse du muscle | |
US20060062827A1 (en) | Nutritional supplement composition and method | |
JP6037595B2 (ja) | 満腹感誘導組成物及びその製造方法 | |
JP2019031466A (ja) | 血糖値上昇の抑制又は改善用組成物 | |
JP6430386B2 (ja) | 体水分保持剤 | |
EP3386316B1 (fr) | Compositions | |
WO2016133036A1 (fr) | Agent d'amélioration de fatigue | |
WO2018123873A1 (fr) | Composition pour favoriser la transpiration | |
WO2018164251A1 (fr) | Composition pour améliorer la résistance physique | |
WO2018139624A1 (fr) | Composition pour favoriser la sécrétion d'insuline | |
EP4304366A1 (fr) | Compositions nutritionnelles et procédés associés | |
WO2018074415A1 (fr) | Composition pour améliorer le coefficient d'efficacité protéique | |
JP2019024389A (ja) | 筋力向上用アミノ酸組成物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14844506 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2015536652 Country of ref document: JP Kind code of ref document: A |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 14844506 Country of ref document: EP Kind code of ref document: A1 |