WO2015025869A1 - 土壌伝染性病害防除剤及びそれを用いた土壌伝染性病害防除方法 - Google Patents

土壌伝染性病害防除剤及びそれを用いた土壌伝染性病害防除方法 Download PDF

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WO2015025869A1
WO2015025869A1 PCT/JP2014/071727 JP2014071727W WO2015025869A1 WO 2015025869 A1 WO2015025869 A1 WO 2015025869A1 JP 2014071727 W JP2014071727 W JP 2014071727W WO 2015025869 A1 WO2015025869 A1 WO 2015025869A1
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soil
disease control
infectious disease
control agent
bacterium
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French (fr)
Japanese (ja)
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前田　広人
毅 吉川
繁明 坂口
弘毅 魏
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Kagoshima University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Definitions

  • the present invention is a soil infectious disease control agent comprising a microorganism having an antagonistic action against a phytopathogenic fungus causing a soil infectious disease as an active ingredient, and prevention or development of a soil infectious disease using the same. It relates to a method for controlling.
  • Soil infectious disease is an agriculturally important infectious plant disease that is transmitted through soil.
  • purple crest rot caused by Helicobasidium mompa is a multi-civilized pathogen that infects most plants except grasses and plants, regardless of plant or woody plant. It is a soil-borne disease. Occurring mainly in East Asia such as Japan and Korea, it causes great damage such as extreme quality degradation and yield reduction of crops. In addition, it has a life span of 5 to 20 years in the soil, and if it is contaminated with this fungus, will it rotate for at least 5 years before it improves to the absence of disease? Need to be fallow.
  • chemical pesticides can also have a significant impact on useful microflora other than the causative organisms of soil infectious diseases targeted by application to soil. Therefore, there is a need for the development of safe and sustainable control methods for soil-borne diseases that replace chemical control methods.
  • Antagonistic microorganisms are microorganisms that suppress the growth and activity of specific microorganisms.
  • ecosystem-based agriculture such as pesticide-free agriculture (or environmental conservation) agriculture
  • antagonistic microorganisms as microbial pesticides suppresses the growth and activity of pathogenic bacteria. It is not an exaggeration to say that the technology for reducing diseases to plants is a dream technology, and its development is eagerly desired (Patent Document 1).
  • Bacillus subtilis is known to antagonize certain pathogenic bacteria that cause plant diseases, and has already been registered as a pesticide in Japan as a control agent for gray mold of eggplant and tomato. Has been.
  • Patent Document 2 discloses a disease control technique for agricultural and horticultural plants containing a spore fraction prepared from a culture of bacteria belonging to the genus Bacillus such as Bacillus subtilis so as to contain 50% by weight or more of spores by dry weight. Is disclosed.
  • Patent Document 3 is composed of the actinomycete Amycolatopsis A1 strain, has no pathogenicity to plants, is easily adaptable to the agricultural field, exhibits high colonization in plants, and has a filamentous shape.
  • a filamentous fungal disease control agent that exhibits an excellent control effect on fungal disease control is disclosed.
  • JP2011-241178 Japanese Patent Laid-Open No. 8-175919 JP 2006-290816
  • the object of the present invention is to develop and provide a microbial pesticide that has a high control effect on soil infectious diseases caused by phytopathogenic fungi, has a low environmental load, and is safe and free of phytotoxicity, and By developing a method to effectively control soil-borne diseases using microbial pesticides, it is possible to reduce work labor and costs, and to provide safe agricultural products stably.
  • the present inventors have obtained a combined action when a photosynthetic bacterium, Rhodopseudomonas bacterium and a soil bacterium, Bacillus bacterium are applied in combination. Has found that it exhibits extremely high antagonistic properties and fertilizer effects against phytopathogenic fungi, which are the causative agents of soil-borne diseases, and has completed the present invention.
  • the present invention is based on the above findings and provides the following.
  • Rhodopseudomonas bacterium for use as an active ingredient of a soil infectious disease control agent against phytopathogenic fungi.
  • Rhodopseudomonas bacterium according to (1), wherein the Rhodopseudomonas bacterium is an international receipt number FERM ABP-19431 or FERM ABP-19432.
  • a Bacillus bacterium for use as an active ingredient of a soil infectious disease control agent against phytopathogenic fungi (1) A Bacillus bacterium for use as an active ingredient of a soil infectious disease control agent against phytopathogenic fungi.
  • a soil infectious disease control agent for phytopathogenic fungi containing photosynthetic bacteria and Bacillus bacteria as active ingredients.
  • Rhodopseudomonas bacterium is a bacterium of international receipt number FERM ABP-19431 or FERM ABP-19432.
  • the phytopathogenic fungi are Helicobasidium, Rosellinia, Fusarium, Pythium, Pyrenochaeta, Monosporacus (Monosporascus), Verticillium, Rhizoctonia, Plasmodiophora, Phitophthora, and Sclerotium
  • the soil infectious disease control agent according to any one of (5) to (9).
  • the soil infectious disease is selected from the group consisting of coat feather disease, root-knot disease, seedling blight disease, wilt disease, root rot disease, silkworm disease, and phytosofra disease.
  • the soil infectious disease control agent according to any one of the above.
  • a soil infectious disease control method wherein the soil infectious disease control agent according to any one of (5) to (11) is applied to soil or a medium.
  • Rhodopseudomonas bacteria or Bacillus bacteria of the present invention the growth and activity of phytopathogenic fungi can be efficiently suppressed.
  • the soil infectious disease control agent of the present invention there is no disease to plants, it exhibits a high control effect on soil infectious diseases caused by phytopathogenic fungi, and has a low environmental impact and is safe.
  • a microbial pesticide free from phytotoxicity can be provided.
  • the soil infectious disease can be effectively controlled using the soil infectious disease control agent of the present invention. Moreover, it can reduce labor and cost, and can provide safe agricultural products stably.
  • the antagonistic action with respect to a plant pathogenic filamentous fungus of a soil infectious disease control agent is shown.
  • PSB Rhodopseudomonas A strain
  • A shows a dish on day 0 of culture and B shows a dish on day 7 of culture.
  • C to F show dishes on day 10 of culture.
  • C is obtained by seeding PSB and Bacillus AUT-8 strain on a filter paper plate and placing purple herb fungus on it.
  • D Bacillus AUT-8 strain was seeded on a filter paper plate and purple herring fungus was placed on it.
  • E shows PSB and Bacillus AUT-9 strains seeded on a filter paper plate, and purple coat rot fungus on them.
  • F is a strain in which only Bacillus AUT-9 strain is seeded on a filter paper plate, and purple herring fungus is arranged thereon.
  • the first aspect of the present invention is a soil infectious disease control agent for phytopathogenic fungi.
  • the soil infectious disease control agent of this aspect can suppress the growth and activity of phytopathogenic fungi targeted by specific bacteria as an active ingredient through the occupying action and antagonism of the bacteria. Therefore, the disease caused by the phytopathogenic fungus of the plant can be reduced by applying it to a desired plant.
  • soil-borne disease control agent refers to a microbial pesticide for the purpose of preventing and controlling the occurrence of soil-borne disease.
  • Microbial pesticides are intended for the purpose of inhibiting the growth of target organisms, suppressing the activity, inhibiting growth, or killing the target organisms that cause disease to plants by using the properties and activities of the microorganisms as active ingredients.
  • the “microorganism” usually refers to a micro-organism that is difficult to recognize with the naked eye, such as bacteria (bacteria; eubacteria) or fungi.
  • the fungus is a group of eukaryotes belonging to the fungal kingdom and is also called a fungus or a filamentous fungus.
  • the fungus includes a unicellular eukaryotic microorganism such as yeast, or a multicellular eukaryotic microorganism such as a filamentous fungus (including mold) that is relatively difficult to recognize with the naked eye.
  • soil infectious disease refers to a disease that develops in plants due to infection with phytopathogenic fungi transmitted through soil or planting medium (including liquid medium).
  • phytopathogenic fungi transmitted through soil or planting medium (including liquid medium).
  • coat rot including purple coat rot and white coat rot
  • root-knot including seedling blight (including rice seed blight)
  • wilt including tomato wilt and eggplant half wilt
  • Root rot white silkworm
  • phytosofla plague Hermaphroditis, particularly purple herb disease, is preferred as an object of prevention and control with the soil infectious disease control agent of this embodiment.
  • the term “phytopathogenic fungus” refers to a microorganism that is a target of the soil infectious disease control agent of this embodiment and that infects a living plant and causes the soil infectious disease.
  • the plant pathogenic filamentous fungi of this embodiment include, for example, Helicobasidium bacteria, Roselinia bacteria, Fusarium bacteria, Pythium bacteria, Pyrenochaeta bacteria, Monosporascus bacteria, Verticillium bacteria, Rhizoctonia bacteria, Plasmodiophora bacteria, Phitophthora bacteria, or Sclerotium bacteria Can be mentioned.
  • Helicobacterium can be a suitable target for the soil infectious disease control agent of this embodiment.
  • H. ⁇ mampa H. brebissonii (or H. ⁇ ⁇ purpureum)
  • H. longisporum have been identified as taxonomic effective species. Both can be targets of the soil infectious disease control agent of this embodiment.
  • H. mompa which is a causative bacterium of purple coat feather disease, is particularly suitable.
  • the soil infectious disease control agent of this embodiment includes an active ingredient that is an essential ingredient and a carrier that is a selective ingredient.
  • the soil infectious disease control agent of this embodiment contains at least two different bacteria, that is, photosynthetic bacteria and Bacillus bacteria as active ingredients.
  • photosynthetic bacteria and Bacillus bacteria as active ingredients.
  • Bacillus bacteria as active ingredients.
  • each bacterium will be specifically described.
  • Photosynthetic bacteria are bacteria that perform photosynthesis, and include red sulfur bacteria, red non-sulfur bacteria, green sulfur bacteria, and green non-sulfur bacteria.
  • the photosynthetic bacterium is an essential active ingredient for the soil infectious disease control agent of the present embodiment, and the type thereof is not particularly limited, but is preferably a red non-photosynthetic bacterium, and particularly preferably a Rhodopseudomonas bacterium. It is. For example, R. faecalis, R. palustris, R. rhenobacensis and the like are included.
  • Rhodopseudomonas bacteria include Rhodopseudomonas A strain (International Receipt No. FERM ABP-19432) and Rhodopseudomonas B strain (International Receipt No. FERM ABP-19431) closely related to R. faecalis. Among them, Rhodopseudomonas A strain (International receipt number FERM ABP-19432) is particularly preferable.
  • the mycological properties of Rhodopseudomonas A strain are alkali-resistant and have bacterial chlorophyll a, neurosporene and lycopene.
  • Rhodopseudomonas B strain The bacteriological properties of Rhodopseudomonas B strain are alkali-tolerant like strain A and have bacterial chlorophyll a and neurosporene.
  • alkali resistance refers to a property having a maximum growth rate mainly at pH 8-10.
  • Rhodopseudomonas A and B strains are described in detail in Japanese Patent Publication No. 3699987, and on July 15, 2003, the National Institute of Technology and Evaluation, Patent Biological Deposit Center (Kazusa Kisarazu City, Chiba Prefecture 292-0818, Japan) 2-5-8, Kamafoot Room 120), deposited in Japan and received as an international deposit on August 15, 2014.
  • two or more kinds of photosynthetic bacteria for example, Rhodopseudomonas A strain and Rhodopseudomonas B strain may be contained.
  • Bacillus bacteria refers to obligate aerobic or facultative aerobic Gram-positive rods that form spores.
  • B. aerophilus, B. siamensis, B. subtilis, B. mojavensis, and B. amyloliquefaciens ) Etc. are included.
  • Bacteria belonging to the genus Bacillus together with photosynthetic bacteria are essential active ingredients for the soil infectious disease control agent of this embodiment.
  • the type of bacteria belonging to the genus Bacillus is not particularly limited, but a species closely related to B. aerophilus in the B. aerophilus or 16S rRNA gene classification is preferable.
  • the Bacillus AUT-8 strain international accession number NITE BP-01678
  • Bacillus AUT-9 strain international accession number NITE BP-01679
  • the soil infectious disease control agent of this aspect contains at least photosynthetic bacteria and Bacillus bacteria as active ingredients and contains them in a living state.
  • the mixing ratio of the photosynthetic bacterium and Bacillus genus bacteria in the soil infectious disease control agent of this embodiment is not particularly limited as long as both bacteria are included, but preferably the photosynthetic bacteria: Bacillus genus bacteria are 1: 1 to 10000: 1.
  • the photosynthetic bacterium and the Bacillus genus bacterium may be in a mixed state at the time of application of the soil infectious disease control agent, and the two bacteria may not necessarily be in a mixed state before the application, for example, during the preservation process.
  • the “viable state” refers to a state in which bacteria can metabolize and / or divide and proliferate. Therefore, spores in Bacillus bacteria are also included in the living state as long as they have germination power.
  • the soil infectious disease control agent may contain dead photosynthetic bacteria and / or Bacillus bacteria.
  • the soil infectious disease control agent of the present embodiment can contain a carrier that is acceptable in an agrochemical formulation as long as it does not inhibit or suppress the survival and physiological activity of photosynthetic bacteria and Bacillus bacteria which are active ingredients.
  • “Agrochemically acceptable carrier” means a substance that facilitates the application of a soil-borne disease control agent, maintains the survival of photosynthetic bacteria and Bacillus bacteria, and antagonism or suppression of phytopathogenic fungi, and / or A substance that controls the action rate of a soil infectious disease control agent and that is not harmful or low in the environment such as soil or water quality and / or animals, particularly humans.
  • the carrier in the soil infectious disease control agent of this embodiment include excipients. Excipients include ground natural minerals, ground synthetic minerals, emulsifiers, dispersants, surfactants, and the like.
  • ground natural minerals examples include kaolin, clay, talc and chalk.
  • Examples of the pulverized synthetic mineral include highly dispersed silica and silicate.
  • emulsifier examples include nonionic emulsifiers and anionic emulsifiers (for example, polyoxyethylene fatty alcohol ethers, alkyl sulfonates, and aryl sulfonates).
  • dispersant examples include lignosulfite waste liquor and methylcellulose.
  • surfactant examples include lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid alkali metal salt, alkaline earth metal salt and ammonium salt, alkylaryl sulfonate, alkyl sulfate, alkyl sulfonate, fat Alcohol sulfates, fatty acids and sulfated fatty alcohol glycol ethers, sulfonated naphthalene and naphthalene derivatives and formaldehyde condensates, naphthalene or naphthalenesulfonic acid and phenol and formaldehyde condensates, polyoxyethylene octylphenyl ether, ethoxylated isoforms Octylphenol, octylphenol, nonylphenol, alkylphenyl polyglycol ether, tributylphenyl polyglycol
  • the soil infectious disease control agent of the present invention can include one or more carriers that are acceptable in agricultural chemical formulations.
  • active ingredients having other pharmacological actions that is, herbicides, fungicides, insecticides, fertilizers (for example, urea, ammonium nitrate, and superphosphates), as long as the active ingredients do not affect bacteria. It can also be included.
  • the form of the soil infectious disease control agent of the present invention is not particularly limited as long as it can retain photosynthetic bacteria and Bacillus bacteria as active ingredients in a living state.
  • it can be in a liquid state, a solid state, or a combination thereof in which each bacterium is suspended in an appropriate solution.
  • a liquid state what is necessary is just to suspend a photosynthetic bacterium and / or a Bacillus genus bacterium in an appropriate solution.
  • Suitable solutions include, for example, water (including sterilized water, deionized water, and ultrapure water), saline, buffers (including phosphate buffer and carbonate buffer), and bacterial cultures thereof.
  • a solid state for example, a granular state, a powder state, and a semi-solid state such as a gel can be mentioned.
  • dosage forms such as liquids, powders, granules, and seedling culture media.
  • the content of photosynthetic bacteria and Bacillus bacteria per predetermined amount of the soil infectious disease control agent of the present invention includes various types of bacteria and combinations thereof, types of plants to be applied, dosage forms, application methods, and the like. Varies depending on conditions.
  • the photosynthetic bacteria and Bacillus bacteria contain a sufficient amount to exert an antagonistic action or inhibitory action against phytopathogenic fungi. This content may be determined in consideration of each condition so that photosynthetic bacteria and Bacillus bacteria can be in a desired amount in the soil per predetermined volume after application within the scope of common general technical knowledge in the field.
  • the content of photosynthetic bacteria and Bacillus bacteria in the soil infectious disease control agent of this embodiment may be in the range of 10 6 to 10 11 cfu / g as the concentration of both bacteria. In this case, it can be diluted 10 to 1000 times with water, physiological saline, buffer, or the like, if necessary, at the time of application.
  • the soil infectious disease control agent of this embodiment it is possible to efficiently suppress the growth and activity of phytopathogenic fungi using photosynthetic bacteria (particularly Rhodopseudomonas bacteria) and Bacillus bacteria as active ingredients.
  • the soil infectious disease control agent of the present invention is not toxic or pathogenic to plants, and has a high control effect against soil infectious diseases caused by phytopathogenic fungi.
  • it is a microbial pesticide containing bacteria that exist in nature as an active ingredient, it is highly safe and has less impact on the environment than chemical pesticides. Even if it is applied to the soil, there is no danger to human livestock, no problems such as environmental pollution and residue on agricultural products. Therefore, it is possible to provide consumers with safe and harmless crops.
  • the resistant bacteria appearance rate of the phytopathogenic filamentous fungus which is a target is very low compared with a chemical pesticide.
  • the soil infectious disease control agent of the present invention also has a plant activity effect.
  • microbial activity in soil is improved and microbial density is increased, so that beneficial soil microorganisms originally inhabiting in soil are activated. It is considered that the microbial community (bacteria flora) works against phytopathogenic fungi and suppresses the occurrence of soil-borne diseases in plants.
  • the second aspect of the present invention is a soil infectious disease control method.
  • the control method according to this aspect is characterized in that the soil infectious disease control agent according to the first aspect is applied to soil or a medium to prevent or control the occurrence of soil infectious diseases in the target plant.
  • the “target plant” is a plant to which a soil infectious disease control agent is applied.
  • the target plant is not limited as long as it is a plant that can cause soil-borne diseases by infection with phytopathogenic fungi.
  • Either angiosperms or gymnosperms may be used.
  • dicotyledonous plants or monocotyledonous plants are included.
  • Monocotyledonous plants include, for example, Poaceae plants.
  • dicotyledonous plants convolvulaceae, roseceae, piaceae, solanaceae, liliaceae, fabaceae plants, cucumbers Examples include Cucurbitaceae plants and Brassicaceae plants.
  • the specific target plant may vary depending on the type of phytopathogenic fungus causing the soil infectious disease.
  • the phytopathogenic fungus is a coat rot, particularly purple potato, sweet potato (Ipomoea batatas)
  • Examples include carrots (Daucus carota subsp. Sativus), potatoes (Solanum tuberosum), asparagus (Asparagus spp), apples (Malus pumila), pears (Pyrus pyrifolia var. Culta, P. bretschneideri, P. communis).
  • the soil infectious disease control method of this aspect includes an application process.
  • the “application step” refers to a step of applying the soil infectious disease control agent of the first aspect to soil or a medium.
  • the “soil” here is not particularly limited as long as the target plant can grow. Usually, soil for planting target plants containing appropriate nutrients (nitrogen, phosphorus, potassium, etc.) and having an appropriate pH value is used. The place of soil is not ask
  • “medium” refers to an artificially prepared medium for planting a target plant. It may be a solid medium such as an agar medium or a liquid medium. Examples of the culture medium include, for example, an isolation bed, a root zone restriction pot, or a nursery.
  • the medium composition may be a medium composition known in the art. It can be selected as appropriate depending on the type of crop to be applied.
  • the application method of the soil infectious disease control agent can be appropriately selected according to the type of soil infectious disease, the type of applied crop, and the like.
  • the soil infectious disease control agent of the present invention may be applied to the soil for planting the target plant, preferably the corresponding part (for example, the heel) of the root group of the target plant.
  • it can be applied by mixing, spraying, or irrigation to a part where the degree of soil infectious disease is high.
  • manure or the like may be used in combination with a soil infectious disease control agent.
  • fertilizer components to be supplemented they can be used in combination with various components of chemical fertilizers, soil conditioners, organic fertilizers, and the like.
  • the application time of the soil infectious disease control agent of the first embodiment is not limited, but is preferably before planting the target crop, for example, before sowing or before planting.
  • photosynthetic bacteria and Bacillus bacteria which are active ingredients, grow in the soil, prevent the invasion of phytopathogenic fungi by occupancy, and already exist This is because by eliminating phytopathogenic fungi by antagonism, the infection rate of the target crops by phytopathogenic fungi can be reduced, and the occurrence of soil-borne diseases can be efficiently prevented or controlled.
  • the timing of planting the target plant in the applied soil is 2 days to 3 weeks after application of the soil infectious disease control agent, preferably 10 days to 2 weeks later.
  • the application rate of the soil infectious disease control agent of the first aspect varies depending on the dosage form, application method, application time, type of target plant, etc. Adjust it.
  • the soil infectious disease control agent is a liquid agent, it may be used at an application rate of 0.5 to 20 tons, preferably 1 to 10 tons per 10 ares of soil.
  • the number of applications of the soil infectious disease control agent of the first embodiment is not limited. If applied before planting the target plant, in principle it is sufficient. However, the soil infectious disease control effect increases by applying multiple times regardless of before and after planting of the target plant. Therefore, in order to form soil disease control soil, it is preferable to increase the application frequency of a soil infectious disease control agent.
  • Photosynthetic bacteria are known to be effective as organic fertilizers (Hiro Kitamura, 1984, photosynthetic bacteria, 347-349). Therefore, it becomes possible to improve the microflora of soil further by combining with normal manure. As a result, the effect of controlling soil-borne diseases caused by phytopathogenic fungi is further improved. From this point, it is preferable that the number of application times of the soil infectious disease control agent is larger.
  • the soil infectious disease can be effectively controlled by applying the soil infectious disease control agent to the soil or the like in which a desired plant is planted. Moreover, it can reduce labor and cost, and can stably provide safe agricultural products to consumers.
  • Rhodopseudomonas A strain indicated by accession number FERM P-19432 (international receipt number FERM ABP-19432) described in Patent Publication 3699987 is used.
  • Rhodopseudomonas strain A is cultured under the conditions described in Japanese Patent Publication No.
  • Bacillus spp. The other active ingredient of the soil infectious disease control agent, Bacillus spp., Is a Bacillus spp. Isolated from the soil of sweet potato fields in Ariake-cho, Kanoya City, Kagoshima, Japan, and the Bacillus spp. (Code strain names AUT-5, -7, -8, -9, -10, and -11) were used. According to the 16S rRNA gene classification, these Bacillus genus bacterial strains are all the same species or very closely related to Bacillus aerophilus.
  • Bacillus strains used in this example were NB liquid medium (1% (w / v) Polypepton, 0.5% (w / v) Meat Extract, 0.2% (w / v) NaCl, pH 7.0) Incubated at 25 ° C. Purple rot fungus, Rhodopseudomonas A strain, and each Bacillus genus bacterial strain were cultured under the above conditions two weeks before the antagonistic test.
  • FIG. 2 shows only process numbers 1 (FIGS. 2A and 2B), 4 (FIG. 2D), 5 (FIG. 2F), 10 (FIG. 2C), and 11 (FIG. 2E).
  • the dish (FIGS. 2B, D, and F) in which purple coat fungus has grown has a dark color due to the elongated red-purple hyphae. That is, this result shows that Rhodopseudomonas sp.
  • a strain alone or Bacillus bacterial strain alone cannot sufficiently suppress the growth and activity of purple rot fungus.
  • AUT-8 or AUT-9 was mixed and combined among Rhodopseudomonas strain A and Bacillus genus strains (FIGS. 2C and 2E)
  • purple herring fungus was hardly able to grow and cultured for 0 days. It was shown that it had almost the same color as the eyes (FIG. 2A).
  • the soil infectious disease control agent contains at least two kinds of bacteria, Rhodopseudomonas bacteria and Bacillus bacteria, as active ingredients, thereby suppressing the growth and activity of phytopathogenic fungi and the soil of the target plant. It became clear that infectious diseases can be prevented or controlled.

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PCT/JP2014/071727 2013-08-23 2014-08-20 土壌伝染性病害防除剤及びそれを用いた土壌伝染性病害防除方法 Ceased WO2015025869A1 (ja)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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CN110679610A (zh) * 2019-08-28 2020-01-14 湖南省植物保护研究所 沼泽红假单胞菌生防菌剂在防治马铃薯晚疫病的应用
CN121109152A (zh) * 2025-10-14 2025-12-12 江苏农林职业技术学院 一种不规则毛霉及其应用

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63107908A (ja) * 1986-05-19 1988-05-12 Fumakiraa Kk 有機組成物
JPH02101005A (ja) * 1988-10-08 1990-04-12 Yamaha Rekurieeshiyon Kk リゾクトニア・ラージパッチの防除方法
JP2004065259A (ja) * 2002-07-23 2004-03-04 Hiroto Maeda 光合成細菌およびラン色細菌
JP2004323460A (ja) * 2003-04-28 2004-11-18 National Agriculture & Bio-Oriented Research Organization 植物病害防除剤及びそれを用いた植物の病害防除法
JP2005206496A (ja) * 2004-01-21 2005-08-04 Idemitsu Kosan Co Ltd 白紋羽病防除剤および白紋羽病の防除方法
JP2007055982A (ja) * 2005-08-26 2007-03-08 Itsuki Co Ltd バチルス属細菌の栄養細胞を有効成分とする植物病害の防除方法および防除剤
WO2012161160A1 (ja) * 2011-05-26 2012-11-29 株式会社エス・ディー・エス バイオテック バチルス属に属する菌株、微生物製剤、及び植物の栽培方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3428851B2 (ja) * 1997-03-14 2003-07-22 照文 荒木 有機質発酵肥料とその使用方法
JP3140430B2 (ja) * 1999-03-09 2001-03-05 株式会社 バイテク バチルス属微生物とその用途

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63107908A (ja) * 1986-05-19 1988-05-12 Fumakiraa Kk 有機組成物
JPH02101005A (ja) * 1988-10-08 1990-04-12 Yamaha Rekurieeshiyon Kk リゾクトニア・ラージパッチの防除方法
JP2004065259A (ja) * 2002-07-23 2004-03-04 Hiroto Maeda 光合成細菌およびラン色細菌
JP2004323460A (ja) * 2003-04-28 2004-11-18 National Agriculture & Bio-Oriented Research Organization 植物病害防除剤及びそれを用いた植物の病害防除法
JP2005206496A (ja) * 2004-01-21 2005-08-04 Idemitsu Kosan Co Ltd 白紋羽病防除剤および白紋羽病の防除方法
JP2007055982A (ja) * 2005-08-26 2007-03-08 Itsuki Co Ltd バチルス属細菌の栄養細胞を有効成分とする植物病害の防除方法および防除剤
WO2012161160A1 (ja) * 2011-05-26 2012-11-29 株式会社エス・ディー・エス バイオテック バチルス属に属する菌株、微生物製剤、及び植物の栽培方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109136155A (zh) * 2018-09-30 2019-01-04 山西永丰生物工程技术有限公司 一种防治果树根腐病的微生物菌剂的制备方法
CN110679610A (zh) * 2019-08-28 2020-01-14 湖南省植物保护研究所 沼泽红假单胞菌生防菌剂在防治马铃薯晚疫病的应用
CN121109152A (zh) * 2025-10-14 2025-12-12 江苏农林职业技术学院 一种不规则毛霉及其应用

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