WO2014178500A1 - Composition cosmétique fonctionnelle comprenant un hydrolysat enzymatique d'écailles de branchiostegus japonicus - Google Patents

Composition cosmétique fonctionnelle comprenant un hydrolysat enzymatique d'écailles de branchiostegus japonicus Download PDF

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WO2014178500A1
WO2014178500A1 PCT/KR2013/009997 KR2013009997W WO2014178500A1 WO 2014178500 A1 WO2014178500 A1 WO 2014178500A1 KR 2013009997 W KR2013009997 W KR 2013009997W WO 2014178500 A1 WO2014178500 A1 WO 2014178500A1
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jade
scales
hydrolyzate
cosmetic composition
test
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PCT/KR2013/009997
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English (en)
Korean (ko)
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문영섭
안용석
문창훈
고창익
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(주)청룡수산
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers

Definitions

  • the present invention relates to a cosmetic composition
  • a cosmetic composition comprising an enzymatic hydrolyzate of jade ( braniostegus japonicus ) scales, and more particularly to a functional cosmetic composition having an antioxidant, anti-wrinkle and whitening effect containing the ingredient as an active ingredient. .
  • Cosmetics refers to all things used for make-up, such as creams and lotions. Cosmetics prescribed by the Pharmacist Act are "articles used by painting, spraying or other similar methods to clean or beautify the human body. It is defined as, “and at the same time, products that are regulated in all aspects, including manufacturing, sales, and advertising.
  • Cosmetics with various functions such as cosmetics to moisturize the skin, cosmetics to reduce the size of pores, cosmetics to remove dead skin cells, cosmetics to relieve wrinkles and cosmetics with whitening function are being released.
  • the demand for the same functional cosmetics is expected to continue to increase.
  • the problem to be solved in the present invention is to provide a functional cosmetic composition comprising an enzymatic hydrolyzate extracted from jade fish scales.
  • the present invention provides a cosmetic composition comprising the enzymatic hydrolyzate of jade ( braniostegus japonicus) scale extract.
  • the enzymatic hydrolyzate is dried (S1) jade dome scales after washing with far infrared rays; (S2) hydrothermal extraction of the dried jade fish scales; (S3) enzymatic hydrolysis of the hydrothermally extracted jade fish scales; (S4) activated carbon filtration of the enzymatic hydrolyzate of the jade fish scales; (S5) filtering the activated carbon filtrate with a 0.4 to 0.5 ⁇ m filtration membrane; (S6) filtering the filtrate by the filter membrane of 0.4 ⁇ 0.5 ⁇ m with an ion exchange resin membrane; (S7) fractionating the ion exchange resin membrane filtrate into an ultrafiltration membrane; And (S8) is preferably prepared comprising the step of lyophilization and pulverization of the fractions.
  • the enzymatic hydrolysis is preferably selected from the group consisting of ⁇ -chymotrypsin, alcalase, neutrase and trypsin.
  • the cosmetic composition preferably exhibits antioxidant, anti-wrinkle and whitening effects.
  • the enzymatic hydrolyzate of the jade dome scale which is an active ingredient of the present invention, exhibits an excellent effect on antioxidant activity, wrinkle improvement and whitening, and is suitable for use as a functional cosmetic composition because it is harmless to the human body as a natural material.
  • Figure 1 shows a flow chart of jade fish scales extraction.
  • Figure 2 is a result of measuring the degree of hydrolysis of the enzymatic gelatin hydrolyzate obtained by using various enzymes after hydrothermal treatment of jade fish scales.
  • Figure 3 is the result of measuring the degree of hydrolysis of jade dome scale alkalase hydrolyzate by substrate / enzyme treatment concentration.
  • Figure 4 is the result of measuring the degree of hydrolysis of jade dome scale alkalase hydrolyzate by substrate concentration.
  • Figure 5 shows the results of the LC-MS analysis of the result of ultrafiltration of the jade dome scale alkalase hydrolyzate.
  • 6A to 6D show the molecular weights of the four peaks on the LC-MS analysis of the result of ultrafiltration of the jade dome scale alcalase hydrolyzate.
  • E1 is an alkalase enzyme hydrolyzate of the jade dome scales
  • A is an ultrafiltration fraction having a molecular weight of 260
  • B is Ultrafiltration fraction with a molecular weight of 189
  • C represents an ultrafiltration fraction with a molecular weight of 223
  • D represents an ultrafiltration fraction with a molecular weight of 288.
  • E1 is an alkalase enzyme hydrolyzate of the jade dome scales
  • A is an ultrafiltration fraction having a molecular weight of 260
  • B denotes an ultrafiltration fraction having a molecular weight of 189
  • C denotes an ultrafiltration fraction having a molecular weight of 223
  • D denotes an ultrafiltration fraction having a molecular weight of 288.
  • Figure 9 is the result of measuring the Elastinase inhibition in order to confirm the wrinkle improvement effect of the enzymatic hydrolyzate of jade dome scales
  • E1 is an alkalase enzyme hydrolyzate of jade dome scales
  • A is an ultrafiltration fraction of molecular weight 260
  • B is the molecular weight 189 ultrafiltration fraction
  • C represents an ultrafiltration fraction with a molecular weight of 223
  • D represents an ultrafiltration fraction with a molecular weight of 288.
  • Figure 10 is the result of measuring the tyrosinase inhibition in order to confirm the whitening activity of the enzymatic hydrolyzate of jade dome scales
  • E1 is an alkalase enzyme hydrolyzate of jade dome scales
  • A is an ultrafiltration fraction having a molecular weight of 260
  • B is 189 molecular weight Phosphorus ultrafiltration fraction
  • C denotes ultrafiltration fraction with molecular weight 223
  • D denotes ultrafiltration fraction with molecular weight 288.
  • MMP-1 Collagenase-1
  • 12a to 12h respectively show the manufacturing process of the essence, nourishing cream, body lotion, rinse, shampoo, body cleanser, lotion, skin comprising the cosmetic composition of the present invention.
  • FIGS. 13A to 13E are photographs showing representative steps of the manufacturing process of the mask pack including the cosmetic composition of the present invention.
  • 21 to 25 show the results of the overall efficacy analysis of the test group with the essence of the present invention.
  • 26 is a result showing the satisfaction with the usability of the test group with the essence of the present invention.
  • the inventors of the present invention confirmed that the enzymatic hydrolyzate obtained from the jade dome scales has antioxidant activity, anti-wrinkle effect and whitening activity, and developed the enzymatic hydrolysates of the jade dome scales having such activity for the use of functional cosmetic composition. It was.
  • the present invention provides a cosmetic composition comprising an enzymatic hydrolyzate of jadedox ( Braniostegus japonicus ) scale extract.
  • the body height is the highest at the very back of the head, tapered toward the back, the contour of the back is almost straight, and the lateral line passes above the center of the side of the body and is almost parallel to the contour of the back.
  • the body is covered with relatively large rectangular comb scales, and the body is generally reddish, with two or three horizontal stripes appearing just above the tip of the pectoral fin. Triangular silver-white spots are clearly visible on the back of the eye, and caudal fin has 5-6 rows of clear yellow vertical stripes.
  • the bottom of 30 ⁇ 200m depth lives on the edge of the continental shelf of sand and mud, and there is a habit of digging holes in sand or sand bottom and living in it. It does not move much but moves north in autumn and south in spring and eats crustaceans such as shrimp, crabs and crayfish, as well as worms, fish and shellfish.
  • the spawning season is from June to October, and eggs are laid in the seabed at the depth of 70-100m before and after the water temperature of 18 °C, and the life span is 8-9 years.
  • Enzymatic hydrolyzate of the jade dome scales of the present invention comprises the steps of (S1) drying the jade dome scales in far infrared rays after washing with water; (S2) hydrothermal extraction of the dried jade fish scales; (S3) enzymatic hydrolysis of the hydrothermally extracted jade fish scales; (S4) activated carbon filtration of the enzymatic hydrolyzate of the jade fish scales; (S5) filtering the activated carbon filtrate with a 0.4 to 0.5 ⁇ m filtration membrane; (S6) filtering the filtrate by the filter membrane of 0.4 ⁇ 0.5 ⁇ m with an ion exchange resin membrane; (S7) fractionating the ion exchange resin membrane filtrate into an ultrafiltration membrane; And (S8) is preferably prepared comprising the step of lyophilization and pulverization of the fractions.
  • Jade dome scales can be easily obtained throughout the Republic of Korea, it is preferable to use to obtain enzymatic hydrolyzate after washing and drying with fresh water. As for the drying, it is effective to use far infrared rays.
  • the enzymatic hydrolyzate may be obtained by treating the domido scales with proteolytic enzymes as it is, or by first extracting the domido scales from hydrothermal and then treating the hydrothermal extract with proteolytic enzymes. In order to increase the yield of the hydrolyzate, it is preferable to perform hydrothermal extraction first.
  • the conditions of hot water extraction are not particularly limited, but preferably 1 to 5 hours at 100 ° C, more preferably 3 hours at 100 ° C.
  • Proteolytic enzymes that can be used in the present invention include, for example, ⁇ -chymotrypsin, alcalase, neutrase, trypsin, and the like, but preferably ⁇ -chymotrypsin or alcalase.
  • Hydrolysis conditions are not particularly limited, but may be subjected to hydrolysis for 3 to 24 hours at the optimum pH and temperature of each enzyme.
  • the hydrolyzate is filtered using activated charcoal to remove off-flavor and peculiar color after hydrolysis.
  • the filtration method using activated carbon may be any known one.
  • the hydrolyzate, which has undergone such multi-step filtration, is fractionated according to the molecular weight using an ultrafiltration membrane, and it is preferable to obtain a fraction having a molecular weight of 5kDa, 10kDa and 30kDa through the ultrafiltration membrane.
  • the molecular weight distribution of the enzymatic hydrolyzate of the jade dome scales showing antioxidant activity, wrinkle improvement and whitening activity varies from 5 kDa or less to 30 kDa.
  • the molecular weight of the enzymatic hydrolyzate suitable for the cosmetic composition of the present invention is most preferably 5 kDa or less.
  • Fractions obtained through the above process is dried through a concentration process, and then stored until milled.
  • the cosmetic composition of the present invention may be prepared in the form of, for example, an essence, nutrition cream, body lotion, rinse, shampoo, body cleanser, lotion, skin, mask pack, and the like, but is not limited thereto. Preparation in this form may be readily performed according to various manufacturing processes known to those skilled in the art.
  • the jade dome scales used in the present invention were obtained from Cheongryong Fisheries Co., Ltd. located in Seogwipo-si, Jeju-do, and dried under far-infrared light after washing with fresh water.
  • Moisture, crude protein, crude fat and ash of dried jade fish scales were measured. According to the AOAC method, the moisture content was measured by 105 °C atmospheric pressure drying method with a significant difference of 0.002g or less, the crude protein content was measured by Micro Kjeldahl method, the ash content was measured by dry method, and the crude fat content was determined by Soxhlet extraction method. Measured.
  • the water content was 8.5 ⁇ 0.01
  • the ash content was 45.0 ⁇ 1
  • the crude protein content was 46.1 ⁇ 0.02
  • the remaining components were 0.4 ⁇ 0.001.
  • Hamanda and Kumagai (1988) analyzed the scales of sardines, a marine fish species, and reported that their contents were similar, with 53.16g of 100g heavy ash and 41g of collagen protein. These results do not show a big difference from the scale of the dome of the present invention, it was confirmed that the scale of the dome is higher in the protein content than other components can be a useful raw material for gelatin extraction.
  • the non-collagen protein was removed by alkali treatment at 10 ° C. for 3 days using 1 N NaOH alkaline solution in 8 times jade dome scales.
  • water was washed with running water and neutralized with 6N HCl.
  • Six times (v / w) of distilled water was added to the neutralized jade dome scales, and hot water was extracted three times at 60 ° C. for three hours.
  • the hot water extracted solution was filtered under reduced pressure using a filter paper (5A 110mm, advantec, Japan), and the filtered gelatin extract solution was concentrated at 60 ° C using a vacuum concentrator, and then lyophilized to be pulverized into powder and used as a sample. .
  • the dried and powdered oxime scale-derived gelatin was hydrolyzed by time using four proteolytic enzymes shown in Table 2 below.
  • the hydrolyzate was further centrifuged (12000 rpm, 15 minutes) to separate the supernatant and the degree of hydrolysis was measured.
  • the degree of hydrolysis of the enzymatic hydrolyzate of jade-derived gelatin was measured by TCA (trichloroacetic acid) method.
  • TCA trichloroacetic acid
  • the reaction mixture after the reaction was centrifuged (12000 rpm, 15 minutes) to take 2 ml from the supernatant, 20% TCA was added thereto in the same amount, centrifuged (3500 rpm, 10 minutes), and then a predetermined amount of the supernatant was removed.
  • 10% TCA soluble nitrogen was measured by Lowry method, and the degree of hydrolysis was calculated from the following equation.
  • the hydrolysis degree of the hydrolyzate of each time zone extracted using four proteolytic enzymes showed a sharp increase in hydrolysis degree up to 6 hours of hydrolysis reaction in all four enzymes.
  • the hydrolysates alkalase hydrolyzed the oxime scale gelatin most effectively, and showed a degree of hydrolysis of about 65% or more after 6 hours of the hydrolysis reaction.
  • hydrolysis was performed according to the substrate-to-enzyme ratio and substrate concentration of the enzymatic hydrolyzate.
  • Substrate-to-enzyme ratios were 10, 20, 50, 100, 200, and 500 (weight / weight), and the enzymatic hydrolyzate concentrations of the gelatin, derived from jade dome scales, were 1%, 3%, 5%, and 10%.
  • the hydrolysis was carried out in the same manner as the method presented above, and the degree of hydrolysis of the hydrolyzate was measured for the examination of all hydrolysis conditions.
  • the degree of hydrolysis was increased as the ratio of oxime scale-derived gelatin to enzyme decreased.
  • the degree of hydrolysis is over 60%, and from 200 it can be seen that the degree of hydrolysis decreases rapidly. From this result, it can be seen that 100 of the various substrate-to-enzyme ratios show the best efficiency for the degree of hydrolysis.
  • the substrate-to-enzyme ratio 100 the results of measuring the degree of hydrolysis according to the substrate concentration are shown in FIG. 4. The lower the concentration of the substrate was, the higher the degree of hydrolysis was. However, when the substrate concentration was 5% or less, the degree of hydrolysis was almost similar. In terms of mass production, a substrate concentration of 5% is considered to be the most appropriate.
  • the alkalase hydrolyzate of the jade dome scale was first filtered through an activated carbon filtration membrane, and then secondarily filtered through a 0.45 ⁇ m diameter filtration membrane. Next, the filtrate was filtered through an ion exchange resin membrane in 3rd order to obtain a final filtrate.
  • the filtrate of the hydrochloric acid-treated dome enzymatic hydrolyzate was 5kDa, 10kDa or less, 30kDa using an ultrafiltration membrane using 5kDa, 10kDa and 30kDa membranes.
  • the following fractions were each prepared.
  • DPPH is a stable model of free radicals. It can be seen that DPPH reduction during the reaction proceeds to scavenging free radicals and predicts the inhibition of the initial reaction of lipid peroxidation. Active oxygen, called harmful oxygen, is known to attack unsaturated fatty acids, which are components of cell membranes, to induce lipid peroxidation reactions and accumulate lipid peroxides, resulting in deterioration of biological function and at the same time causing aging and adult diseases. Antioxidant activity by plant components and extracts has been reported. In addition, H 2 O 2 can also measure the degree of antioxidant reaction by the degree of reduction of H 2 O 2 as a representative active oxygen.
  • collagen and elastin form the network structure and maintain the elasticity of the skin.
  • the elasticity and the luster decrease due to internal and external stress such as age and ultraviolet rays, and the elastin mesh is caused by the overexpressed elastinase. If the structure is broken, the skin sags and wrinkles are generated, resulting in skin aging. Therefore, skin aging can be suppressed by inhibiting the activity of elastinase, which is an enzyme of elastin, one of the main causes of skin aging.
  • the inhibitory activity of elastinase was measured using Cannell et al. To examine the anti-wrinkle effect of oxime scale gelatin hydrolyzate and four ultrafiltration fractions. Elastinase inhibitory activity was measured by elastinase substrate VIII as a substrate for 15 minutes at room temperature to determine the amount of p-nitoanilide produced. 160 ⁇ l of each test solution diluted with 0.2 M tris-HCl (pH 8.0) buffer, 20 ⁇ l of 5 mM elastinase substrate VIII (CALBIOCHEM) solution and 20 ⁇ l of 10 ⁇ l / ml elastinase (sigma) enzyme were added in this order and reacted at 25 ° C. for 15 minutes. After absorbance was measured at 410nm using an ELISA reader (TECAN, AT / sunrise R / C, Switzerland).
  • the inhibitory activity of tyrosinase was measured using Fuller's method. Add 220 ⁇ l of 0.1M phosphate buffer (pH 6.5), 20 ⁇ l of sample solution, and 20 ⁇ l of tyrosinase (sigma) solution (1500 U / ml ⁇ 2000 U / ml). 40 ⁇ l of 1.5mM trysine (sigma) solution was added to the solution and reacted at 37 ° C. for 10-15 minutes, and the absorbance was measured at 490 nm using an ELISA reader (TECAN, AT / sunrise R / C, Switzerland). 0.1 M phosphate buffer (pH 6.5) was used instead of the sample solution as the blank sample solution.
  • Melanin synthesis is based on tyrosine, one of the amino acids, and the reaction after 3,4-dihydroxyphenylalanin (DOPA) and DOPA quinone by tyrosinase is largely pheomelanin, which determines reddish brown or yellow color, and eumelanin, which determines brown to brown color. It is divided into, and whitening effect can be expected by inhibiting melanin production by inhibiting the activity of tyrosinase, a major enzyme of melanin production.
  • DOPA 3,4-dihydroxyphenylalanin
  • MMP-1 Collagenase-1
  • MMP-1 which acts to degrade skin collagen
  • Toxic scale protein peptide toxicity test was commissioned by Biotoxtech specialized institution, detailed results are as follows.
  • OBJECTIVES To investigate the toxic response of oral administration of oxydome hydrolyzate, a test substance, in male and female Sprague-Dawley rats, and to obtain an approximate lethal dose.
  • the group consisted of 2,000 mg / kg of test substance and 2 groups of control group (injection water), and five male and female mice were orally administered once. For 14 days after administration, general symptoms and body weights were observed, and euthanized by euthanasia at the end of the observation period. No deaths were observed in the 2,000 mg / kg male and female groups. In addition, the effects of test substance administration on general symptoms, body weight and necropsy were not recognized. Under the conditions of this test, a single oral dose of oxime scale hydrolyzate to rats is estimated to exceed approximately 2,000 mg / kg of male and female.
  • the skin irritation test of test substance jade fish scale enzyme hydrolyzate was examined using three 16-week-old male NZW rabbits. The administration sites of the left and right each 2 sites and the sum 4 sites were set to the back of the rabbit, and 2 of them were irradiated and the other 2 were used as abrasion sites. 0.5 g of the test substance raw material was applied to each non-abrasive and abrasion site for 24 hours to blockage patch. The skin response was assessed according to Draize's Skin Response Evaluation Table at 24, 48 and 72 hours after dosing, and the primary skin irritation index (PII) was determined for 24 and 72 hours after dosing to determine skin irritation. .
  • PII primary skin irritation index
  • Eye irritation testing of test substance jade fish scale enzyme hydrolyzate was reviewed using six 16-week-old male NZW rabbits.
  • 0.1 g of test substance was injected into three rabbits' right eye conjunctival sac, and eye lesions such as cornea, iris and conjunctiva were observed at 1, 24, 48 and 72 hours after administration.
  • the face wash group was additionally administered in the same manner as the non-wash face group using three animals, and after washing for 30 seconds after administration, the face wash effect was confirmed.
  • the eye irritation of the test substance was evaluated according to the grade of the eye lesion of Draize, and the degree of eye irritation was classified by referring to Guillot's eye irritation evaluation table.
  • the test substance jade fish scale hydrolyzate under this test condition is judged to be non-irritant to the ocular mucosa of rabbit.
  • test material Maximization method
  • guinea pigs was conducted to examine the possibility of allergic to human skin.
  • test substance group was firstly sensitized by intradermal administration of 50% test substance, and secondly sensitized by 100% test substance for 48 hours.
  • test substance jade fish scale hydrolyzate is a substance without skin sensitization under the present test conditions.
  • test substance jade fish scale enzyme hydrolyzate The presence and extent of phototoxicity of the test substance jade fish scale enzyme hydrolyzate to the skin were examined using Hartley guinea pigs.
  • the test group consisted of three groups: test substance group (5), negative control group (5) and positive control group (5). 22 cm of administration sites were set on the right and left sides of the guinea-pig skin of the test substance group, the negative control group, and the positive control group. 90% test substance was applied to the test substance group, water for injection to the negative control group, and 0.1% 8-Methoxypsoralen (8-MOP) to the positive control group was applied to each administration site.
  • 8-MOP 8-Methoxypsoralen
  • UV-A was irradiated so that the final energy was about 10 J / cm 2 with the light irradiation site on the left side and the non-light irradiation site on the right side.
  • Skin reactions were evaluated 24, 48 and 72 hours after light irradiation, and the presence or absence of phototoxicity was determined. Skin reactions such as erythema and edema were not observed in all animals at 24, 48 and 72 hours after irradiation at 90% of the test substance dose. At all observation times at the injection site of the negative control group, no skin reactions such as erythema and edema were observed in all animals.
  • Stripping, open coating of the administration material, and UV-A irradiation were performed once a day for 5 consecutive days.
  • Yagi was caused by UV-A irradiation on day 21 after administration of 90% test substance and water for injection in test substance and negative control group and 0.1% CP and ethanol in positive control group.
  • Skin reactions were evaluated 24 and 48 hours after firing light irradiation. In the test substance group and the negative control group, skin reactions such as erythema and edema were not observed in all animals at 24 and 48 hours after the irradiated light irrespective of the irradiated area of 90% test substance and water for injection.
  • Cosmetics in the form of essences, nourishing creams, body lotions, rinses, shampoos, body cleansers, lotions, skins and mask packs were prepared comprising the jade fish scale hydrolyzate and ultrafiltration fractions of the present invention. Specific manufacturing processes are shown in FIGS. 12A-12H and 13A-13E.
  • the purpose of this study is to evaluate the wrinkle-improving effect and skin safety of the product by using the test product for 12 weeks in women who are 30 years old or older or who have already developed wrinkles.
  • wrinkles begin to be produced in accordance with SMA of the Dermatological Institute of Dermatology, or those who have indicated their intention to explain the purpose and method, expected efficacy and adverse reactions of the test should be filled out. Participated in the test.
  • Evaluation was performed by visual evaluation of crow's feet before and after 4, 8, and 12 weeks of product use and measurement of skin wrinkle parameters (R-value) using Skin Visiometer® SV600 (C + K, Germany), VISIA®. (Canfield, USA) was used to evaluate skin wrinkle improvement and skin safety by taking photographs, evaluating subjects, and investigator observation and questionnaires.
  • the comparison between groups at each time point was confirmed by correcting the difference between two groups before using the product using covariance analysis (ANCOVA), and the before and after comparison was confirmed using RM ANOVA.
  • ANCOVA covariance analysis

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Abstract

La présente invention concerne une composition cosmétique comprenant un hydrolysat enzymatique d'écailles de Branchiostegus japonicus et, plus particulièrement, une composition cosmétique fonctionnelle, présentant des effets antioxydants, antirides et blanchissants, comprenant les composants au titre de principes actifs. L'hydrolysat enzymatique d'écailles de Branchiostegus japonicus, qui sont des principes actifs de la présente invention, présente une excellente activité antioxydante et d'excellents effets antirides et blanchissants, et cette substance naturelle est inoffensive pour l'organisme humain, ce qui la rend adaptée à une utilisation de la composition cosmétique fonctionnelle.
PCT/KR2013/009997 2013-05-01 2013-11-06 Composition cosmétique fonctionnelle comprenant un hydrolysat enzymatique d'écailles de branchiostegus japonicus WO2014178500A1 (fr)

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CN107260572A (zh) * 2017-06-20 2017-10-20 青岛金典生化器材有限公司 一种抗菌鱼鳞蛋白面膜及其制备方法
CN107233227A (zh) * 2017-06-20 2017-10-10 青岛金典生化器材有限公司 一种茶氨酸改性鱼鳞蛋白面膜的制备方法
KR102062273B1 (ko) 2018-09-18 2020-01-03 (주)뷰티화장품 천연 펄을 포함하는 하이드로겔 팩의 제조방법

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