WO2014148057A1 - 培養装置 - Google Patents
培養装置 Download PDFInfo
- Publication number
- WO2014148057A1 WO2014148057A1 PCT/JP2014/001633 JP2014001633W WO2014148057A1 WO 2014148057 A1 WO2014148057 A1 WO 2014148057A1 JP 2014001633 W JP2014001633 W JP 2014001633W WO 2014148057 A1 WO2014148057 A1 WO 2014148057A1
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- WIPO (PCT)
- Prior art keywords
- heat
- dew condensation
- box
- culture
- heat transfer
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Definitions
- the present disclosure relates to a culture apparatus for culturing a culture such as cells and microorganisms in a culture region.
- the culture apparatus includes a heater for heating the inside of the incubator on which the humidifying dish is placed.
- the incubator is controlled to maintain the inside of the incubator at a predetermined temperature (for example, 37 ° C.).
- the inside of the vessel is maintained at a predetermined humidity (for example, 95% RH) corresponding to the predetermined temperature.
- a bottom heater for heating the water in the humidifying dish a heater for heating the inside of the incubator other than the humidifying dish, and a heater attached to the heat insulating door that can be freely opened and closed to the heat insulating box body
- the culture apparatus which suppresses is disclosed (for example, refer patent document 1).
- the culture apparatus includes a temperature sensor for detecting the temperature in the incubator and a temperature sensor for detecting the outside air temperature. Based on the detection results of these two temperature sensors, the three types of heaters are installed. Each is controlled individually.
- the temperature of the water in the humidifying dish and the temperature in the incubator are predetermined by controlling the heater as in the culture apparatus disclosed in Patent Document 1 described above.
- a corresponding detection accuracy is required for each temperature sensor.
- the predetermined relationship between the two temperatures is such that the inside of the incubator can be maintained at a humidity close to the saturated water vapor density while suppressing condensation in a place that may affect the culture in the incubator.
- the temperature of the water in the humidifying dish is lower than the temperature in the incubator.
- the present disclosure provides a culture apparatus that can suppress dew condensation and suppress adverse effects on the culture.
- the culture apparatus of the present disclosure for solving the above problems controls a substantially box-shaped heat insulation box main body that forms a culture space for culturing a sample of cells, microorganisms, and the like, and humidity of the culture space.
- a heat transfer condensation member, and the heat transfer condensation member is configured such that a condensation portion disposed inside the culture space is inclined downward toward the one end, and the one end is condensed on the surface of the condensation portion. It is the structure arrange
- the culture apparatus of the present disclosure includes a substantially box-shaped heat insulation box body that internally forms a culture space for culturing a sample of cells, microorganisms, and the like, and humidified water for controlling the humidity of the culture space.
- a humidifying dish that is stored and disposed at the bottom of the culture space; and a heat transfer dew condensation member that penetrates through the heat insulation box body, has one end disposed in the culture space and the other end disposed outside the heat insulation box body.
- the heat transfer dew condensation member is disposed at a position where the one end of the dew condensation part disposed inside the culture space introduces the dew condensation water condensed on the surface of the dew condensation part to the humidifying dish, and
- the box body has a substantially box-shaped inner box that forms the culture space, and a substantially box-shaped outer box that surrounds the outer periphery of the inner box, and a portion that penetrates the inner box in the heat transfer dew condensation member And a first heat-insulating sealing material having a heat insulating property and a hydrophobic property between the inner box and the inner box
- the first heat-insulating sealing material is provided with dew condensation that an extension part extending into the culture space inclines downward toward one end of the heat transfer dew condensation member and adheres to the periphery of the extension part. It is the structure arrange
- the culture apparatus according to the present disclosure is disposed at a position where condensed water condensed on the surface of the dew condensation part or the first heat insulating sealing material is introduced into the humidifying dish, moisture condensed on the dew condensation part or the first heat insulating sealing material is removed. Since it can flow down and put into a humidifier and repeatedly used as humidified water, dew condensation on the wall surface of the culture space can be suppressed and adverse effects on the culture can be suppressed.
- FIG. 5 is a cross-sectional explanatory diagram of an incubator for explaining air circulation centered on a culture space and a duct as viewed from the right side as another embodiment of the culture apparatus of an example of the present disclosure.
- FIG. 5 is a plan view illustrating a relationship in which a cooling unit of a heat transfer dew condensation member is disposed in a heat conducting state on an outer box of a heat insulating box body as another embodiment of the culture apparatus of an example of the present disclosure.
- it is a top view explaining the relationship by which the cooling part of the heat-transfer dew condensation member is arrange
- the culture apparatus 1 includes a substantially box-shaped heat insulating box body formed by an inner box 22 in which a culture space 4 for culturing a sample such as a cell or a microorganism is disposed, and a humidity of the culture space 4 Humidifying dish 15 for storing the humidified water for controlling the culture and disposed at the bottom of the culture space 4 and the heat insulating box main body 2, one end is disposed inside the culture space 4 and the other end is outside the heat insulating box main body 2.
- a heat transfer dew condensation member 35 disposed on the inner box 22 and a portion of the heat transfer dew condensation member 35 that penetrates the inner box 22 and the inner box 22 are disposed via a heat insulating sealing material 36.
- the portion of the sealing material 36 that extends into the culture space 4 has a configuration in which an inclined surface that is inclined obliquely forward and downward so as to introduce the condensed water adhering to the periphery into the humidifying dish 15 is formed.
- the culture apparatus 1 includes a left-open door (specifically, an outer door 7 and an inner door 3), and heat insulation having an opening 2 ⁇ / b> A on the front surface.
- the box body 2 has a substantially box shape in which a culture space 4 for culturing samples such as cells and microorganisms is formed.
- a culture space 4 for culturing samples such as cells and microorganisms is formed.
- it is composed of a metal outer box 21 and a heat insulating material 24 disposed inside the outer box 21, and an air layer (so-called air jacket) 25 exists on the inner side.
- An inner box 22 having a front opening made of stainless steel is arranged.
- a culture space 4 having a front opening is formed in the heat insulation box main body 2 by the inner box 22, and the culture space 4 becomes an area for culturing samples such as cells and microorganisms.
- Heater heaters 37 for heating the culture space 4 are disposed on the left and right side surfaces, the top surface, the bottom surface, and the back surface of the inner box 22.
- the heat insulating box body 2 having the opening 2A on the front surface is formed with a culture space 4 for culturing samples such as cells and microorganisms.
- a heat insulating door 7 serving as an outer door for preventing heat from entering from the front opening 2A of the culture space 4 is attached to one side portion (left side in the drawing) of the heat insulating box body 2 so as to be openable and closable.
- a magnet-containing gasket 8 is annularly arranged on the peripheral edge of the back side surface.
- the gasket 8 When the heat insulating door 7 is closed, the gasket 8 is in close contact with the peripheral edge of the front opening 2A of the heat insulating box body 2, whereby the front opening 2A is hermetically closed by the heat insulating door 7, and from the front opening 2A of the culture space 4 Prevent outside air entry.
- the culture space 4 is partitioned and formed by a stainless inner box 22 having an opening on the front surface.
- the left and right side surfaces, the top surface, and the rear surface of the inner box 22 have a heat insulating box body 2 and an air layer (so-called air jacket) 25. And it is arrange
- the front opening of the inner box 22 is configured to be opened and closed by a transparent door 3 as an inner door, and the culture space 4 is formed by a space substantially surrounded by the inner box 22 and the transparent door 3.
- the left side of the transparent door 3 is supported on the inner box 22 by a hinge so as to be opened and closed.
- An inner peripheral edge 22 of the inner box 22 is provided with an annular elastic seal member 2B.
- the culture space 4 is divided vertically by a plurality of shelves 5 (here, four shelves are divided into five).
- Culture apparatus 1 is, for example, if the CO 2 incubator, in many cases to set and maintain the concentration of CO 2 in the order of 5%, the culture space of CO 2 gas after occlusion of the door in order to control the concentration of CO 2 4 is supplied.
- the rear duct 11 ⁇ / b> A and the bottom surface are spaced apart from the back wall and the bottom wall of the inner box 22 in order to form gas passages K of air containing CO 2 and the like along the back surface and the bottom surface, respectively.
- a duct 11 composed of a duct 11B is disposed, and a gas containing CO 2 or the like in the culture space 4 is sucked from a suction port 12 formed in the upper part of the rear duct 11A, and is provided on the front and side surfaces of the bottom duct 11B.
- the forced circulation of the air which blows off in the culture space 4 from the blower outlet 13 is performed.
- a circulation fan 14 is disposed in the duct 11 (here, the upper portion) for forced circulation of a gas containing CO 2 and the like.
- the blower 14 includes a fan 14A, a motor 14B, and a shaft 14C.
- the motor 14B is disposed in a machine chamber 19 on the outer rear surface of the heat insulating box body 2 described later, and the shaft 14C is a motor 14B of the machine chamber 19.
- humidifying water 16 As a humidifying dish 15 for humidifying the inside of the culture space 4, humidifying water (that is, humidified water) 16 is provided in the duct 11 between the bottom surface of the culture space 4 and the bottom wall 11 ⁇ / b> B and the bottom wall of the inner box 22.
- a humidifying tray 15 having an upper surface opening for storing water is disposed, and is heated by a heater 37 disposed on the outer bottom surface of an inner box 22 made of metal such as stainless steel to evaporate water.
- the humidifying tray 15 is efficiently circulated in the gas passage K such as CO 2 formed by the circulation fan 14 and the duct 11. It becomes possible to make it.
- a motor as a driving unit for the circulation fan 14, a gas supply unit 17 for supplying CO 2 gas to the culture space 4, and electrical components such as a control board (not shown).
- a machine room 19 for arranging an electrical box 38 or the like for housing the housing is formed by a back cover 26 that covers the back of the outer box 21.
- the gas supply means 17 includes a gas supply pipe 17A, an on-off valve 17B, a filter 17C, and the like, and the distal end portion of the gas supply pipe 17A faces the gas passage K.
- CO 2 gas supplied from the gas supply pipe 17A can be injected.
- an ultraviolet lamp 27 is arranged in the gas passage K.
- the culture apparatus 1 includes a heat transfer dew condensation member 35 in which a dew condensation part 31 at one end and a cooling part 32 at the other end are integrated via a communication part 30 at a predetermined location of the culture apparatus 1. Is installed.
- the heat transfer dew condensation member 35 is a heat pipe in which hydraulic fluid is sealed, a round bar of a predetermined length made of a good heat conductive material such as aluminum, or a flat plate of a predetermined length made of a good heat conductive material such as aluminum. Either may be sufficient.
- FIG. 9 shows a configuration of a heat pipe 35 which is a kind of heat transfer dew condensation member 35.
- the heat pipe 35 vacuum seals a small amount of liquid (working fluid) in a rod-shaped airtight container and has a capillary structure on the inner wall.
- (Wick) 33 is provided, and the working fluid evaporates (absorption of latent heat of vaporization) in the dew condensation unit 31, and the evaporated vapor moves in the direction of the cooling unit 32.
- the vapor condenses in the cooling unit 32 and releases latent heat of vaporization.
- the condensed liquid returns to the dew condensation part 31 by capillary action. Such a series of phase changes occur continuously, so that heat moves quickly.
- the dew condensation member 35 may be either a round bar of a predetermined length made of a metallic good heat conductive material such as aluminum or silver, or a flat plate of a predetermined length made of a good heat conductive material such as aluminum or silver. Good.
- an antimicrobial coating is formed on the surface thereof by antibacterial plating or antibacterial anodized to obtain an effect of suppressing propagation of germs.
- heat transfer dew condensation member 35 may generate patina, so that a treatment such as plating so that the patina does not appear on the surface is necessary.
- a round bar heat transfer dew condensation member 35 made of a good heat conductive material such as aluminum which is a kind of heat transfer dew condensation member 35 is employed.
- the heat transfer dew condensation member 35 has its cooling part 32 arranged outside the heat insulation box main body 2 and the communication part 30 is arranged in the culture space 4 in the heat insulation box main body 2 through the heat insulation box main body 2 and the inner box 22. Therefore, the dew condensation part 31 is mounted so as to be disposed in the gas passage K in the duct 11A.
- the cooling unit 32 is disposed in the machine room 19 on the back side of the culture apparatus 1 so as not to be damaged by an external force from the periphery of the culture apparatus 1.
- the connecting part 30 is disposed at least between the outer box 21 made of metal and the inner box 22 made of stainless steel via a heat insulating sealing material 36. ing. Since the heat-insulating sealing material 36 is thus interposed, the gas flowing through the gas passage K does not leak, the culture space 4 is not adversely affected, and no condensation occurs on the contact portion between the connecting portion 30 and the inner box 22. . Moreover, the thermal influence of the outer box 21 and the inner box 22 can be suppressed.
- an electronic cooling device 40 for cooling the cooling unit 32 to an appropriate temperature is attached to the cooling unit 32.
- an electronic cooling element 41 referred to as a Peltier element that cools the cooling unit 32 by the Peltier effect.
- a heat sink 42 for radiating the heat is provided as the electronic cooling device 40.
- the electronic cooling element 41 called a Peltier element forms a cooling / heating unit with a plurality of PN-junction semiconductor elements, and the adjacent N-type semiconductors and P-type semiconductors are arranged adjacent to each other in a state where N-type semiconductors and P-type semiconductors are arranged alternately.
- a heat good conduction plate to constitute a heat absorption part (cooling side)
- the other end side is connected by a heat good conduction plate to constitute a heat radiation part (heating side)
- this is a known configuration in which an N-type semiconductor and a P-type semiconductor are connected in series.
- the heat sink 42 is made of aluminum in which a plurality of plate-like radiating fins 42B are integrally formed with the plate-like base portion 42A with a space on the upper surface of the plate-like base portion 42A.
- the electronic cooling element 41 has the heat absorption part (cooling side) attached to the upper surface of the cooling part 32 of the heat transfer condensation member 35 in a heat conductive state.
- the cooling section 32 of the heat transfer condensation member 35 has a flat mounting surface on the upper surface, and a heat conductive member such as a thin heat conductive rubber sheet is provided on the mounting surface via heat radiation grease.
- a cooling side of the electronic cooling element 41 is arranged.
- the heat sink 42 (heated side) of the electronic cooling element 41 has a plate-like base of the heat sink 42 acting as a radiator via a heat dissipating grease or a heat conductive member such as a thin heat conductive rubber sheet. Part 42A is arranged.
- the mounting screw 44 is inserted into the plate-like base portion 42 ⁇ / b> A of the heat sink 42 through the cooling portion 32 from below the cooling portion 32 of the heat transfer dew condensation member 35. Tighten together.
- the cooling section 32, the electronic cooling element 41, and the heat sink 42 of the heat transfer dew condensation member 35 are integrated into a heat conducting state.
- the cooling unit 32 is cooled by the electronic cooling element 41 in order to cause condensation on the surface of the condensation part 31 while keeping the condensation part 31 of the heat transfer condensation member 35 at the dew point temperature.
- the cooling unit 32 is disposed on the side of the electrical box 38 so that the heat influence generated from the heat generating components in the electrical box 38 is small.
- the connecting portion 30 of the heat transfer condensation member 35 is in a state where it does not contact the outer box 21 and the inner box 22, and the through hole 21 ⁇ / b> P of the outer box 21, the heat insulating sealing material 36, and the inner box 22. It penetrates the through hole 22P.
- the heat insulating sealing material 36 is a hydrophobic body formed by integrally forming a main body 36A (second heat insulating sealing material 36A) and a tip sealing portion 36B (first heat insulating sealing material 36B) at the tip thereof.
- a high member is preferable, and polyacetal resin is one of them.
- hydrophobic rubber, fluorine resin, or the like may be used as the hydrophobic material.
- the heat insulating sealing material 36 is in contact with the inner surface of the back wall of the outer box 21 with the back surface of the main body portion 36A closing the through hole 21P, and the main body portion 36A passes through the heat insulating material 24 and passes through the back surface of the inner box 22.
- a tip seal portion 36B that contacts the back surface of the wall and extends forward from the main portion 36A extends through the through hole 22P of the inner box 22 into the gas passage K.
- the heat transfer dew condensation member 35 is held through the main portion 36A and the tip seal portion 36B of the heat insulating seal material 36 in an inclined state in which the connecting portion 30 is lowered from the rear to the front. Yes.
- the gas flowing through the gas passage K does not leak from the arrangement portion of the heat transfer dew condensation member 35, the culture space 4 is not adversely affected by the gas leakage, and the heat transfer dew condensation member 35 is communicated. Condensation on the inner box 22 via the part 30 can be suppressed, and the thermal influence of the outer box 21 and the inner box 22 on the heat transfer condensation member 35 can be suppressed.
- the heat transfer dew condensing member 35 is connected to the gas passage K in the duct 11A so that moisture condensed on the dew condensing unit 31 and the connecting unit 30 in the vicinity thereof flows down into the humidifying tray 15.
- 30 is bent downward to form a bent portion 34, and the portion of the heat transfer condensation member 35 extending from the bent portion 34 to the condensation portion 31 is configured downward so as to be parallel to the corresponding duct 11A. is there.
- moisture condensed on the portion of the heat insulating sealing material 36 that extends into the duct 11, that is, moisture condensed on the tip seal portion 36 ⁇ / b> B exposed to the gas passage K flows downward and enters the humidifying tray 15. Therefore, the tip seal portion 36B extends right above the humidifying pan 15 with the peripheral surfaces including the left and right surfaces as well as the left and right surfaces forming an inclined surface that is inclined downward toward the front. ing.
- FIG. 10 shows another embodiment.
- the portions denoted by the same reference numerals as those shown in FIGS. 1 to 9 are the same functional portions, and thus detailed description thereof will be omitted and different configurations will be described.
- the tip of the dew condensation part 31 is inclined toward the inside of the humidifying dish 15, and the lowermost end of the dew condensation part 31 in the horizontal direction is located inside the humidifying dish 15 from the edge of the humidifying dish 15. Is disposed at a position that reliably enters the inside of the humidifying tray 15 when it flows downward. That is, the tip of the dew condensation part 31 is inclined so as to be away from the back surface of the heat insulation box body 2.
- the lowermost end of the dew condensation rod 31 can ensure a certain distance from the back surface of the heat insulation box body 2.
- the heat transfer dew condensation member 35 penetrates the back surface of the heat insulation box body 2, but the side surface may penetrate.
- FIG. 11 shows still another embodiment.
- the heat transfer dew condensation member 35 shown in FIG. 11 is not horizontally inclined but horizontally arranged. Specifically, the heat transfer dew condensation member 35 arranges the cooling part 32 outside the heat insulation box main body 2 and arranges the connecting part 30 in a horizontal state through the heat insulation box main body 2 and the inner box 22 to form dew condensation.
- the part 31 is mounted so as to be disposed in the gas passage K in the duct 11A.
- the cooling unit 32 is disposed in the machine room 19 of the culture apparatus 1 so as not to be damaged by an external force from the periphery of the culture apparatus 1.
- the connecting portion 30 is disposed between the stainless steel inner box 22 and the portion where the connecting portion 30 penetrates the inner box 22 with a heat insulating sealing material 36 interposed therebetween.
- the heat insulating sealing material 36 is a synthetic resin material in which the main body portion 36A and the front end sealing portion 36B of the front end portion are formed by integral molding, and the back surface of the main body portion 36A is the back wall of the outer box 21. Abutting on the inner surface, penetrating the heat insulating material 24 and abutting on the back surface of the back wall of the inner box 22, a tip seal portion 36 B extending forward from the main portion 36 A penetrates the back wall of the inner box 22 and passes through the gas passage K. It extends in. As shown in the figure, the heat transfer dew condensation member 35 is held through the main portion 36A and the tip seal portion 36B of the heat insulating seal material 36 with the connecting portion 30 in a horizontal state from the rear to the front.
- the heat transfer dew condensing member 35 is connected to the gas passage K in the duct 11A so that moisture condensed on the dew condensing unit 31 and the connecting unit 30 in the vicinity thereof flows down into the humidifying tray 15.
- 30 is bent downward to form a bent portion 34, and the portion of the heat transfer condensation member 35 extending from the bent portion 34 to the heat input portion 31 is configured to be parallel to the corresponding duct 11A. It is.
- moisture condensed on the portion of the heat insulating sealing material 36 that extends into the duct 11, that is, moisture condensed on the tip seal portion 36 ⁇ / b> B exposed to the gas passage K flows downward and enters the humidifying tray 15. Therefore, the tip seal portion 36B extends right above the humidifying pan 15 with the peripheral surfaces including the left and right surfaces as well as the left and right surfaces forming an inclined surface that is inclined downward toward the front. ing.
- the portion where the connecting portion 30 penetrates the inner box 22 communicates with the stainless inner box 22 via the heat insulating sealing material 36. Since the portion 30 is arranged, the gas flowing through the gas passage K does not leak from the arrangement portion of the heat transfer dew condensation member 35, the culture space 4 is not adversely affected by the gas leakage, and the heat transfer dew condensation member 35 It is possible to suppress dew condensation on the inner box 22 via the communication unit 30, and to suppress the thermal influence of the outer box 21 and the inner box 22 on the heat transfer dew condensation member 35.
- the heat absorbing part (cooling part) of the electronic cooling element 41 is attached in a heat conducting state in the same manner as described above. For this reason, by the same control as described above, dew condensation is generated on the surface of the dew condensation part 31, and the dew condensation water is introduced into the humidifying tray 15 and can be used again for humidification.
- the cooling part 32 of the heat transfer dew condensation member 35 is extended to The cooling part 32 of the heat transfer dew condensation member 35 is attached to the left side surface, the right side surface, or the back surface of the back cover 26 to be covered by a heat conductive member such as a heat-dissipating grease or a thin heat conductive rubber sheet. Can be radiated by the back cover 26.
- the cooling unit 32 has a shape that makes contact with a plurality of surfaces of the outer box 21, and the cooling unit 32 is in contact with the outer box 21 via a heat conductive member such as heat radiation grease or a thin heat conductive rubber sheet.
- the cooling part 32 is dissipated by the outer box 21 by being attached to the outside, and the condensation part 31 is cooled by the heat radiation from the cooling part 32, so that the moisture condensed on the surface of the condensation part 31 is disposed below. It can be introduced into the dish 15.
- the size, shape, dimensions, number, etc. of the heat transfer dew condensation member used in the present disclosure vary depending on the capacity, shape, size, culture, etc. of the culture apparatus.
- the culture apparatus 1 controls the humidity of the substantially box-shaped heat insulation box body 2 that forms a culture space 4 for culturing a sample of cells, microorganisms, and the like, and the culture space 4.
- a humidifying tray 15 that is stored at the bottom of the culture space 4 and the heat insulating box main body 2, one end is disposed inside the culture space 4 and the other end is outside the heat insulating box main body 2.
- a heat transfer condensing member 35 disposed in the contact portion 30 (condensation portion) disposed inside the culture space 4 is inclined downward toward the one end.
- the one end is arranged at a position where the condensed water condensed on the surfaces of the communication unit 30 and the dew condensation unit 31 is introduced into the humidifying tray 15.
- the moisture condensed on the communication part 30 and the dew condensation part 31 flows down and is put into the humidification dish 15, and it can be used repeatedly as the humidification water 16, the dew condensation on the wall inner surface of the culture space 4 is suppressed, and the culture product The adverse effect on can be suppressed.
- the heat insulation box main body 2 has the substantially box-shaped inner box 22 which forms the said culture space 4, the substantially box-shaped outer box 21 surrounding the outer periphery of the said inner box 22, Between the portion of the heat transfer dew condensation member 35 that penetrates the side surface of the inner box 22 and the inner box 22. ) Is arranged. Thereby, even if dew condensation is caused on the portion of the heat insulating sealing material 36 due to the hydrophobicity of the heat insulating sealing material 36, the condensed water can flow down quickly. Further, due to the heat insulating property of the heat insulating sealing material 36, it is possible to suppress condensation on the inner box 22 through the heat transfer dew condensation member 35, and to suppress the thermal influence of the inner box 22 on the heat transfer dew condensation member 35.
- the heat-insulating sealing material 36 has a tip seal portion 36 ⁇ / b> B (extension portion) extending into the culture space 4 downward toward one end side of the heat transfer dew condensation member 35.
- the condensed water adhering to the periphery of the tip seal portion 36 ⁇ / b> B is introduced into the humidifying tray 15.
- the dew condensation can be suppressed and adverse effects on the culture can be suppressed, and the water introduced into the humidifying tray 15 can be used repeatedly as humidified water.
- the culture apparatus 1 controls the humidity of the substantially box-shaped heat insulation box main body 2 that forms a culture space 4 for culturing a sample of cells, microorganisms, and the like, and the culture space 4.
- a humidifying dish 15 which is stored at the bottom of the culture space 4 and the side of the heat insulation box body 2 is penetrated and one end is disposed inside the culture space 4 and the other end is disposed outside the heat insulation box body 2.
- a heat transfer dew condensation member 35, and the heat transfer dew condensation member 35 introduces into the humidifying dish 15 the dew condensation water in which one end of the dew condensation part 31 disposed inside the culture space is condensed on the surface of the dew condensation part 31.
- the heat insulating box body 2 is disposed at a position, and has a substantially box-shaped inner box 22 that forms the culture space 4 and a substantially box-shaped outer box 21 that surrounds the outer periphery of the inner box 22, and a heat transfer condensation member Insulation having a heat insulating property and a hydrophobic property between a portion penetrating the inner box 22 in 35 and the inner box 22
- the sealing material 36 is disposed, and the heat-insulating sealing material 36B is such that the tip seal portion 36B (extension portion) extending into the culture space 4 is inclined downward toward one end side of the heat transfer condensation member 35, and the tip seal
- the condensed water adhering around the part 36 ⁇ / b> B is introduced into the humidifying tray 15.
- the tip seal member 36 ⁇ / b> B is inclined downward toward the front. Even if dew condensation occurs on the portion of the conductive sealing material 36, the dew condensation water can flow down quickly. Further, due to the heat insulating property of the heat insulating sealing material 36, it is possible to suppress condensation on the inner box 22 through the heat transfer dew condensation member 35, and to suppress the thermal influence of the inner box 22 on the heat transfer dew condensation member 35.
- the tip seal member 36 ⁇ / b> B is preferably provided up to the bent part 34 or the dew condensation part 31 so as to cover almost all the horizontal communication part 30 in the culture space 4. Thereby, dew condensation water can be quickly flowed down.
- positioned between the said outer box 21 and the said inner box 22 in the heat-transfer dew condensation member 35 is covered with 36 A of 2nd heat insulation sealing materials which have heat insulation. ing.
- positioned between the said outer box 21 and the said inner box 22 in the heat transfer dew condensation member 35 is suppressed in the temperature effect from the inner box 22 and the outer box 21, and it extends in the culture space 4. It is possible to sufficiently obtain a dew condensation effect on the portion to be applied.
- the first heat insulating sealing material 36B and the second heat insulating sealing material 36A are integrally formed. Thereby, formation of the heat-insulating sealing material which effectively suppresses the temperature influence from the peripheral part on the heat transfer dew condensation member 35 and promotes the flow of dew condensation water to the extension part extending into the culture space 4. In addition, it is possible to obtain an attachment operation and a cost reduction effect.
- the heat transfer dew condensation member 35 is an electronic cooling element 41 that can cool the heat transfer dew condensation member 35 by a Peltier effect on the cooling unit 32 disposed outside the heat insulating box body 2. And the moisture condensed on the surface of the dew condensation part 31 by being cooled by the electronic cooling element 41 is introduced into the humidifying tray 15 disposed below.
- the drive voltage of the electronic cooling element 41 there is an effect that it is possible to control to a temperature state that causes dew condensation on the surface of the dew condensation part 31.
- the tip of the dew condensation part 31 is inclined toward the inside of the humidifying dish 15. That is, the tip of the dew condensation part 31 is inclined so as to be away from the back surface of the heat insulation box body 2. Thereby, even if the dew condensation part 31 is brought close to the vicinity of the back surface of the heat insulation box main body 2, the dew condensation water can be surely flowed down into the humidifying tray 15.
- the culture apparatus 1 is configured such that the heat transfer dew condensation member 35 is in contact with the outer box 21 by the cooling unit 32 disposed outside the heat insulating box body 2.
- the dew condensation part 31 is cooled by the heat radiation from the cooling part 32, and moisture condensed on the surface of the dew condensation part 31 is introduced into the humidifying tray 15 disposed below.
- the cooling unit 32 and the outer box 21 are preferably brought into contact with each other via a heat conductive member such as heat radiation grease or a thin heat conductive rubber sheet. Thereby, the heat radiation performed by the cooling unit 32 through the outer box 21 becomes effective.
- the culture apparatus 1 is provided with a cover member 26 that covers at least the cooling part 32 of the heat transfer condensation member 35 outside the heat insulation box body 2. As shown in FIG. The portion 32 is radiated by contacting the cover member 26, and the moisture condensed on the surface of the dew condensation portion 31 when the dew condensation portion 31 is cooled by the heat radiation from the cooling portion 32 is disposed below. Further, it may be introduced into the humidifying tray 15. As a result, the cooling unit 32 can be protected from being damaged by the external force from the periphery of the culture apparatus 1 by the cover member 26, and the cover member 26 can be used for heat dissipation of the cooling unit 32. The cost can be reduced without being provided.
- the cooling unit and the cover member 26 are preferably brought into contact with each other via a heat conductive member such as heat radiation grease or a thin heat conductive rubber sheet. Thereby, the heat radiation performed by the cooling unit 32 through the cover member 26 is effective.
- a culture apparatus is provided with a heat transfer dew condensation member including a dew condensation unit and a cooling unit at a predetermined location.
- a heat transfer dew condensation member including a dew condensation unit and a cooling unit at a predetermined location.
- dew condensation occurs on the dew condensation unit, Since it is made to flow downward and put into a humidifying dish and can be used repeatedly as humidified water, dew condensation is suppressed and adverse effects on the culture are suppressed, and the circulation fan in the duct is operated to operate in the culture space.
- the present invention has a remarkable effect that it can be operated without causing unevenness of temperature and humidity, and can provide an economical culture apparatus with low production cost.
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Abstract
Description
この培養装置は、培養器内の温度を検出するための温度センサと外気温を検出するための温度センサを備えており、これら2つの温度センサの検出結果に基づいて、前記3種類のヒータをそれぞれ個別に制御するようになっている。
また、本開示の培養装置は、細胞や微生物等の試料の培養を行うための培養空間を内部に形成する略箱状の断熱箱本体と、前記培養空間の湿度を制御するための加湿水を貯溜し前記培養空間の底部に配置される加湿皿と、前記断熱箱本体を貫通し一端が前記培養空間の内部に配置され他端が前記断熱箱本体の外部に配置される伝熱結露部材と、を備え、前記伝熱結露部材は、前記培養空間の内部に配置される結露部の前記一端が前記結露部の表面に結露した結露水を前記加湿皿へ導入させる位置に配置され、前記断熱箱本体は、前記培養空間を形成する略箱状の内箱と、前記内箱の外周を囲む略箱状の外箱と、を有し、前記伝熱結露部材における前記内箱を貫通する部分と前記内箱との間には断熱性かつ疎水性を有する第1断熱性シール材が配置され、前記第1断熱性シール材は、前記培養空間内に延出する延出部が前記伝熱結露部材の一端側へ向かって下方へ傾斜し、前記延出部の周囲に付着する結露水を前記加湿皿へ導入させる位置に配置される構成である。
培養空間4のガス濃度を制御するためにガス供給管17Aから供給されるCO2ガスを噴射させることができる。培養空間4内を流通する気体及び加湿皿15内の水16の殺菌効果を得るために、気体通路K内に紫外線ランプ27を配置している。
以下に記載する実施形態では、伝熱結露部材35の一種であるアルミニウム等の良熱伝導材で構成した所定長さの丸棒の伝熱結露部材35を採用した場合について記載する。
本実施形態に係る培養装置1は、細胞や微生物等の試料の培養を行うための培養空間4を内部に形成する略箱状の断熱箱本体2と、前記培養空間4の湿度を制御するための加湿水を貯溜し前記培養空間4の底部に配置される加湿皿15と、前記断熱箱本体2を貫通し一端が前記培養空間4の内部に配置され他端が前記断熱箱本体2の外部に配置される伝熱結露部材35と、を備え、前記伝熱結露部材35は、前記培養空間4の内部に配置される連絡部30(結露部)が前記一端へ向かって下方へ傾斜し、かつ、前記一端が前記連絡部30及び結露部31の表面に結露した結露水を前記加湿皿15へ導入させる位置に配置される構成である。
これにより、連絡部30及び結露部31に結露した水分を下方に流下させて加湿皿15に入れ、加湿水16として繰り返し使用できるので、培養空間4の壁内面への結露を抑制して培養物に対する悪影響を抑制することができる。
これにより、断熱性シール材36の疎水性により、断熱性シール材36の部分に結露しても、その結露水は速やかに流下させることができる。更に、断熱性シール材36の断熱性により、伝熱結露部材35を介して内箱22に結露することを抑制でき、伝熱結露部材35に対する内箱22の熱影響を抑制できる。
これにより、断熱性シール材36の培養空間4内に延出する先端シール部36Bに結露しても、飛散することなく速やかに加湿皿15へ導入させることができるため、培養空間4の壁面への結露を抑制して培養物に対する悪影響を抑制することができ、加湿皿15へ導入された水は加湿水として繰り返し使用できる。
また本実施形態に係る培養装置1は、細胞や微生物等の試料の培養を行うための培養空間4を内部に形成する略箱状の断熱箱本体2と、培養空間4の湿度を制御するための加湿水を貯溜し培養空間4の底部に配置される加湿皿15と、断熱箱本体2の側面を貫通し一端が培養空間4の内部に配置され他端が断熱箱本体2の外部に配置される伝熱結露部材35と、を備え、伝熱結露部材35は、培養空間の内部に配置される結露部31の一端が結露部31の表面に結露した結露水を加湿皿15へ導入させる位置に配置され、断熱箱本体2は、培養空間4を形成する略箱状の内箱22と、内箱22の外周を囲む略箱状の外箱21と、を有し、伝熱結露部材35における内箱22を貫通する部分と内箱22との間には断熱性かつ疎水性を有する断熱性シール材36が配置され、断熱性シール材36Bは、培養空間4内に延出する先端シール部36B(延出部)が伝熱結露部材35の一端側へ向かって下方へ傾斜し、先端シール部36Bの周囲に付着する結露水を加湿皿15へ導入させる。
これにより、図10の他の実施形態のように、たとえ伝熱結露部材35の連絡部30が傾斜していなくても、先端シール部材36Bが前方に向かって下方へ傾斜しているため、断熱性シール材36の部分に結露しても、その結露水は速やかに流下させることができる。更に、断熱性シール材36の断熱性により、伝熱結露部材35を介して内箱22に結露することを抑制でき、伝熱結露部材35に対する内箱22の熱影響を抑制できる。また、連絡部30が水平の場合、先端シール部材36Bは、培養空間4内の水平な連絡部30を略すべて覆い曲折部34又は結露部31まで設けられることが好ましい。これにより、結露水を速やかに流下させることができる。
これにより、伝熱結露部材35における前記外箱21と前記内箱22との間に配置される部分は、内箱22及び外箱21からの温度影響が抑制され、培養空間4内に延出する部分への結露効果を十分に得ることができる。
これにより、伝熱結露部材35に対する周辺部からの温度影響の抑制と、前記培養空間4内に延出する延出部への結露水の流下促進とを効果的に行う断熱性シール材の形成及び取り付け作業、並びにコストダウン効果を得ることができる。
これにより、電子冷却素子41の駆動電圧を可変することにより、結露部31の表面に結露を生じさせる温度状態に制御できる効果がある。
これにより、結露部31を断熱箱本体2の背面近傍まで近づけたとしても、結露した水分を確実に加湿皿15内に流下させることができる。
これにより、冷却部32の放熱に外箱21を利用できるため、電子冷却素子41を設けない低コスト化とすることができる。
これにより、冷却部32が外箱21を通して行われる放熱が効果的となる。
これにより、冷却部32は、カバー部材26によって培養装置1の周囲からの外力によって損傷を受けないように保護できると共に、冷却部32の放熱にカバー部材26を利用できるため、電子冷却素子41を設けない低コスト化とすることができる。
これにより、冷却部32がカバー部材26を通して行われる放熱が効果的となる。
2 断熱箱本体
2A 開口
3 透明内扉
4 培養空間
5 棚
7 断熱扉
8 ガスケット
11 ダクト
11A 背面ダクト
11B 底面ダクト
14 循環用送風機
15 加湿皿
16 加湿水
17 ガス供給手段
17A ガス供給管
21 外箱
22 内箱
24 断熱材
25 空気層(エアージャケット)
30 連絡部
31 結露部
32 冷却部
33 毛細管構造(ウイック)
34 曲折部
35 伝熱結露部材
36 断熱性シール材
36A 断熱性シール材の主体部(第2断熱性シール材)
36B 断熱性シール材の先端シール部(第1断熱性シール材)
40 電子冷却装置
41 電子冷却素子
42 ヒートシンク
Claims (18)
- 細胞や微生物の試料の培養を行うための培養空間を内部に形成する略箱状の断熱箱本体と、
前記培養空間の湿度を制御するための加湿水を貯溜し前記培養空間の底部に配置される加湿皿と、
前記断熱箱本体を貫通し一端が前記培養空間の内部に配置され他端が前記断熱箱本体の外部に配置される伝熱結露部材と、を備え、
前記伝熱結露部材は、前記培養空間の内部に配置される結露部が前記一端へ向かって下方へ傾斜し、かつ、前記一端が前記結露部の表面に結露した結露水を前記加湿皿へ導入させる位置に配置される、
培養装置。 - 前記断熱箱本体は、前記培養空間を形成する略箱状の内箱と、前記内箱の外周を囲む略箱状の外箱と、を有し、前記伝熱結露部材における前記内箱を貫通する部分と前記内箱との間には断熱性かつ疎水性を有する第1断熱性シール材が配置される、請求項1に記載の培養装置。
- 前記第1断熱性シール材は、前記培養空間内に延出する延出部が前記伝熱結露部材の一端側へ向かって下方へ傾斜し、前記延出部の周囲に付着する結露水を前記加湿皿へ導入させる、請求項2に記載の培養装置。
- 前記一端の先端部が前記加湿皿の内部へ向かって傾斜し、水平方向における前記先端部の最下端が前記加湿皿の縁部より前記加湿皿の内側に配置される、請求項1乃至3のいずれかに記載の培養装置。
- 前記伝熱結露部材における前記外箱と前記内箱との間に配置される部分は、断熱性を有する第2断熱性シール材で覆われている、請求項3又は4に記載の培養装置。
- 前記第1断熱性シール材と前記第2断熱性シール材とは一体形成されている、請求項5に記載の培養装置。
- 前記伝熱結露部材は、前記断熱箱本体の外部に配置される冷却部にペルチェ効果によって前記伝熱結露部材を冷却可能な電子冷却素子が設けられ、かつ、前記電子冷却素子によって冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、請求項1乃至6のいずれかに記載の培養装置。
- 前記伝熱結露部材は、前記断熱箱本体の外部に配置される冷却部が前記外箱と当接することにより放熱され、前記冷却部からの放熱によって前記結露部が冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、請求項7に記載の培養装置。
- 前記断熱箱本体の外部には、少なくとも前記伝熱結露部材の冷却部を覆うカバー部材が設けられ、前記冷却部は、前記カバー部材と当接することにより放熱され、前記冷却部からの放熱によって前記結露部が冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、請求項7に記載の培養装置。
- 細胞や微生物の試料の培養を行うための培養空間を内部に形成する略箱状の断熱箱本体と、
前記培養空間の湿度を制御するための加湿水を貯溜し前記培養空間の底部に配置される加湿皿と、
前記断熱箱本体を貫通し一端が前記培養空間の内部に配置され他端が前記断熱箱本体の外部に配置される伝熱結露部材と、を備え、
前記伝熱結露部材は、前記培養空間の内部に配置される結露部の前記一端が前記結露部の表面に結露した結露水を前記加湿皿へ導入させる位置に配置され、
前記断熱箱本体は、前記培養空間を形成する略箱状の内箱と、前記内箱の外周を囲む略箱状の外箱と、を有し、
前記伝熱結露部材における前記内箱を貫通する部分と前記内箱との間には断熱性かつ疎水性を有する第1断熱性シール材が配置され、
前記第1断熱性シール材は、前記培養空間内に延出する延出部が前記伝熱結露部材の一端側へ向かって下方へ傾斜し、前記延出部の周囲に付着する結露水を前記加湿皿へ導入させる、培養装置。 - 前記断熱箱本体は、前記培養空間を形成する略箱状の内箱と、前記内箱の外周を囲む略箱状の外箱と、を有し、前記伝熱結露部材における前記内箱を貫通する部分と前記内箱との間には断熱性かつ疎水性を有する第1断熱性シール材が配置される、請求項10に記載の培養装置。
- 前記第1断熱性シール材は、前記培養空間内に延出する延出部が前記伝熱結露部材の一端側へ向かって下方へ傾斜し、前記延出部の周囲に付着する結露水を前記加湿皿へ導入させる、請求項11に記載の培養装置。
- 前記一端の先端部が前記加湿皿の内部へ向かって傾斜し、水平方向における前記先端部の最下端が前記加湿皿の縁部より前記加湿皿の内側に配置される、請求項10乃至12のいずれかに記載の培養装置。
- 前記伝熱結露部材における前記外箱と前記内箱との間に配置される部分は、断熱性を有する第2断熱性シール材で覆われている、請求項10乃至13のいずれかに記載の培養装置。
- 前記第1断熱性シール材と前記第2断熱性シール材とは一体形成されている、請求項14に記載の培養装置。
- 前記伝熱結露部材は、前記断熱箱本体の外部に配置される冷却部にペルチェ効果によって前記伝熱結露部材を冷却可能な電子冷却素子が設けられ、かつ、前記電子冷却素子によって冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、請求項10乃至15のいずれかに記載の培養装置。
- 前記伝熱結露部材は、前記断熱箱本体の外部に配置される冷却部が前記外箱と当接することにより放熱され、前記冷却部からの放熱によって前記結露部が冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、請求項16に記載の培養装置。
- 前記断熱箱本体の外部には、少なくとも前記伝熱結露部材の冷却部を覆うカバー部材が設けられ、前記冷却部は、前記カバー部材と当接することにより放熱され、前記冷却部からの放熱によって前記結露部が冷却されることにより前記結露部の表面に結露させた水分を下方に配置された前記加湿皿に導入させる、求項16に記載の培養装置。
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DE102017104508B3 (de) * | 2017-03-03 | 2018-03-08 | Adolf Kühner Ag | Verfahren zur Befeuchtung eines Inkubators und Inkubator |
WO2020049968A1 (ja) * | 2018-09-06 | 2020-03-12 | Phc株式会社 | 培養装置 |
CN109974401A (zh) * | 2019-04-22 | 2019-07-05 | 东北大学 | 一种真空干燥箱 |
CN112406980A (zh) * | 2020-12-18 | 2021-02-26 | 浙江师范大学 | 一种微生物标本转运装置 |
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- 2014-03-20 WO PCT/JP2014/001633 patent/WO2014148057A1/ja active Application Filing
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Also Published As
Publication number | Publication date |
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EP2963105B1 (en) | 2017-05-03 |
US9677041B2 (en) | 2017-06-13 |
JPWO2014148057A1 (ja) | 2017-02-16 |
EP2963105A1 (en) | 2016-01-06 |
CN105073973B (zh) | 2018-12-28 |
CN105073973A (zh) | 2015-11-18 |
EP2963105A4 (en) | 2016-03-23 |
JP5887022B2 (ja) | 2016-03-16 |
US20160010048A1 (en) | 2016-01-14 |
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