WO2014108937A1 - Nouveau lactobacille - Google Patents

Nouveau lactobacille Download PDF

Info

Publication number
WO2014108937A1
WO2014108937A1 PCT/JP2013/000098 JP2013000098W WO2014108937A1 WO 2014108937 A1 WO2014108937 A1 WO 2014108937A1 JP 2013000098 W JP2013000098 W JP 2013000098W WO 2014108937 A1 WO2014108937 A1 WO 2014108937A1
Authority
WO
WIPO (PCT)
Prior art keywords
strain
lactic acid
nite
lactobacillus
pediococcus acidilactici
Prior art date
Application number
PCT/JP2013/000098
Other languages
English (en)
Japanese (ja)
Inventor
輝彦 井上
大道 原
Original Assignee
株式会社タカギ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社タカギ filed Critical 株式会社タカギ
Priority to PCT/JP2013/000098 priority Critical patent/WO2014108937A1/fr
Priority to JP2014556204A priority patent/JP5988455B2/ja
Publication of WO2014108937A1 publication Critical patent/WO2014108937A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the present invention relates to a novel lactic acid bacterium having excellent lactic acid production ability and high temperature resistance and use thereof.
  • Lactic acid bacteria are known to have various functions, and are used in various industrial and industrial situations, such as composting, food processing, and medical treatments that ferment compost materials such as garbage and sludge. .
  • Patent Document 1 discloses a method for producing a fertilizer including a step of lactic acid fermentation of raw garbage with lactic acid bacteria to produce lactic acid.
  • Patent Document 2 lactic acid bacteria having an immunity increasing action against pathogenic bacteria in animal bodies have been reported.
  • lactic acid bacteria are generally known to be vulnerable to high temperatures. Therefore, when lactic acid bacteria are used as an active ingredient in foods and pharmaceuticals, or when lactic acid bacteria are used as part of a process in various applications (for example, composting), temperature control has been considered indispensable.
  • the optimum temperature of ordinary lactic acid bacteria is about 30 to 35 ° C., and in the field of food processing and the like, there is a risk that the various bacteria will propagate and contaminate the food when contaminated.
  • the lactic acid bacteria described in Patent Document 2 below which is said to have temperature resistance, have a reproductive power of about half at around 45 ° C., and are hardly able to reproduce at 50 ° C.
  • an object of the present invention is to provide a novel strain of lactic acid bacteria having excellent lactic acid production ability and high temperature resistance. Furthermore, this invention aims at providing the method of using the said strain.
  • the present inventors diligently studied to solve the above-mentioned problem.
  • various lactic acid bacteria contained in Japanese compost the present inventors have an excellent ability to produce lactic acid and have an extremely excellent high-temperature resistance. A new strain was found.
  • the present invention has been completed by making further improvements based on such findings.
  • the lactic acid strain according to the present invention is a novel lactic acid bacterium that has lactic acid-producing ability and has high-temperature resistance that does not die even at 60 ° C.
  • the lactic acid bacterium of the present invention can be used more efficiently and safely in various scenes. For example, when used for food processing, it is possible to process at a high temperature, thereby reducing the risk of food contamination. Moreover, in processes such as composting of organic waste using lactic acid bacteria, the waste decomposition process can be shortened by using the lactic acid bacteria of the present invention that grow even in a high temperature range.
  • the applications of the conventional lactic acid bacteria can be further expanded.
  • FIG. 1 is a photograph showing the results of Example of Test Example 3.
  • FIG. 2 is a photograph showing the result of a comparative example of Test Example 3.
  • the lactic acid bacteria in the present embodiment are characterized by having lactic acid-producing ability and high-temperature resistance that does not die even at 60 ° C.
  • the lactic acid producing ability refers to the ability to produce lactic acid (and acetic acid) using saccharides such as glucose, fructose, and sucrose as fermentation sources.
  • high temperature resistance that does not die even at 60 ° C. means that it does not die even if exposed to about 2 hours under the condition of 60 ° C.
  • “Death” refers to a state in which microorganisms cannot grow when cultured at a medium and temperature suitable for microorganisms after high-temperature treatment.
  • said "under 60 degreeC conditions” is “under the condition where composting of organic substance is progressing at 60 degreeC.”
  • the lactic acid bacteria of the present invention are used for reducing the volume of organic matter, for example, reducing the volume of garbage of organic waste, the above-mentioned “under the condition of 60 ° C.” "Under the conditions”.
  • the lactic acid bacteria according to the present embodiment preferably have a high temperature resistance that does not die even at 65 ° C., and further preferably has a high temperature tolerance that does not die even at 70 ° C.
  • lactic acid bacteria having such high-temperature resistance By using lactic acid bacteria having such high-temperature resistance, it is considered that the number of scenes where lactic acid bacteria can be used increases in composting and food processing processes. In Pediococcus and Lactobacillus that have been conventionally used for food processing, since such high-temperature resistance is not known, the lactic acid bacterium of this embodiment is very useful.
  • the lactic acid bacteria in this embodiment are resistant to acidic conditions and can survive even at pH 4.
  • lactic acid bacteria examples include Pediococcus acidilactici TT12-G5 strain, Pediococcus acidilactici TT12-PG2 strain, Lactobacillus fermentum TT12-18h1 strain, Lactobacillus rhamnosus TT12-S1. It is selected from a strain, Lactobacillus zeae TT12-S4 strain, Lactobacillus casei TT12-18h4 strain, or a mutant thereof. All of these strains are related to Pediococcus and Lactobacillus, which have been established with safety, and are excellent in safety.
  • Each of the above lactic acid strains is a lactic acid bacterium obtained from Japanese compost, and is deposited with the Patent Microorganism Depositary Center for Product Evaluation Technology.
  • the deposit numbers are: Pediococcus acidilactici TT12-G5 (NITE BP-1430), Pediococcus acidilactici TT12-PG2 (NITE BP-1431), Lactobacillus fermentum TT12-18h1 Strains (NITE BP-1448), Lactobacillus zeae TT12-S4 (NITE BP-1433), Lactobacillus rhamnosus TT12-S1 (NITE BP-1432), Lactobacillus casei TT12-18h4 (NITE BP- 1429).
  • the nucleotide sequence of the ITS region of the lactic acid bacterium of this embodiment is SEQ ID NO: 1 for Pediococcus acidilactici TT12-G5 strain, SEQ ID NO: 2 for Pediococcus acidilactici TT12-PG2 strain.
  • Bacillus fermentum TT12-18h1 strain SEQ ID NO: 3 Lactobacillus rhamnosus TT12-S1 strain SEQ ID NO: 4
  • Lactobacillus zeae TT12-S4 strain SEQ ID NO: 5
  • Lactobacillus casei TT12-18h4 strain Is as shown in SEQ ID NO: 6.
  • lactic acid bacteria and their mutants according to this embodiment have excellent lactic acid production ability and high temperature resistance, and therefore can be used in products and processes under high temperatures, further expanding the applications of conventional lactic acid bacteria, At the same time, it is thought that process efficiency can be improved.
  • these lactic acid bacteria there is also a strain having extremely excellent high-temperature resistance that can survive even after being exposed to a high temperature of 80 ° C. for 2 hours.
  • TT12-G5 strain and TT12-PG2 strain produce lactic acid using glucose and acetic acid using fructose. All the strains are related species of Pediococcus known to perform homolactic fermentation, but the TT12-G5 strain and TT12-PG2 strain according to this embodiment have the characteristic of heterofermentation. Have.
  • the TT12-G5 strain and the TT12-PG2 strain have a growth temperature of 30 ° C. to 60 ° C., and an optimum temperature of 50 ° C. That is, at 50 ° C., it exhibits metabolic activity (fermentation) and grows. Since there is no lactic acid bacterium showing activity at such a high temperature, the TT12-G5 strain and TT12-PG2 strain can be applied to various uses such as composting and food processing.
  • the TT12-G5 strain and the TT12-PG2 strain are characterized by secreting an organic acid that dissolves calcium phosphate.
  • the hydroxyapatite which is the main component of the animal bone can be decomposed.
  • phosphorus which is one of the main components of fertilizer, is a resource that is feared to be depleted, and effective utilization of phosphorus resources is required.
  • Animal bones calcium phosphate
  • phosphorus is sparingly soluble, composting so far has reached the molecular level. It has not been used effectively because it is difficult to disassemble.
  • the lactic acid bacteria of the present embodiment secrete an acid that dissolves calcium phosphate as described above, it is possible to increase the number of waste items that can be treated as compared with conventional organic waste decomposition.
  • the TT12-G5 strain and the TT12-PG2 strain are very resistant to acidic conditions and can survive even in the vicinity of pH 1.
  • TT12-18h1 strain produces lactic acid and acetic acid using glucose, fructose, or sucrose.
  • TT12-18h1 strain has a growth temperature of 30 ° C. to 50 ° C. and an optimum temperature of 40 ° C.
  • the TT12-18h1 strain has a characteristic of secreting an acid that dissolves calcium phosphate when fructose is used as a substrate.
  • the hydroxyapatite which is the main component of the animal bone can be decomposed.
  • TT12-S1 and TT12-S4 strains -Cell shape: Neisseria gonorrhoeae-Colony shape: Circular, slightly rough-Colony color: Milky white
  • TT12-S1 strain and TT12-S4 strain produce lactic acid using glucose, but their lactic acid producing ability is very high.
  • the TT12-S1 strain and the TT12-S4 strain have a growth temperature of 30 ° C. to 50 ° C., and an optimum temperature of 40 ° C.
  • the TT12-S1 strain and the TT12-S4 strain are also characterized by secreting an acid that dissolves calcium phosphate.
  • the hydroxyapatite which is the main component of the animal bone can be decomposed.
  • TT12-18h4 strain produces lactic acid using glucose, but its lactic acid producing ability is very high.
  • TT12-18h4 strain has a growth temperature of 30 ° C. to 50 ° C., and an optimum temperature of 40 ° C.
  • Each lactic acid bacterium of this embodiment can grow on the same medium and culture conditions as those of conventionally known pediococcus and lactobacilli, except for the growth temperature.
  • BCP agar medium yeast extract 2.5 g, peptone 5.0 g, glucose 1.0 g, polysorbate 80 1.0 g, L-cysteine 0.1 g, bromcresol purple 0 .06 g, Kanten 15.0 g distilled water (1 L), etc.
  • BCP agar medium yeast extract 2.5 g, peptone 5.0 g, glucose 1.0 g, polysorbate 80 1.0 g, L-cysteine 0.1 g, bromcresol purple 0 .06 g, Kanten 15.0 g distilled water (1 L), etc.
  • the lactic acid bacteria of this embodiment have adhesiveness, it is preferable to use a solid medium rather than a liquid medium because the growth rate is improved.
  • a solid medium rather than a liquid medium because the growth rate is improved.
  • an attached scaffold such as a porous material such as activated carbon, it can be used without solid culture.
  • the culture (growth) temperature varies slightly depending on the strain, but all are cultured at a temperature higher than that of a general lactic acid bacterium.
  • the culture period can be satisfactorily grown in 1 to 2 days.
  • the lactic acid bacteria of this embodiment can grow at pH 4.0 to 8.0 under anaerobic conditions. However, if the pH is too low, there is a possibility that the lactic acid bacteria cannot grow because the physiological activity function of the microorganism is lowered. Moreover, when pH is too high, it tends to be unable to grow because an anaerobic microorganism other than lactic acid bacteria tends to grow. Therefore, it is desirable to culture under conditions of pH 6.0 to 7.5, particularly preferably pH 6.8 to 7.0.
  • the lactic acid bacteria according to the present embodiment include Pediococcus acidilactici TT12-G5 strain, Pediococcus acidilactici TT12-PG2 strain, Lactobacillus fermentum TT12-18h1 strain, Lactobacillus. Also included are mutants of Rhamnosus TT12-S1 strain, Lactobacillus zeae TT12-S4 strain, Lactobacillus casei TT12-18h4 strain.
  • the mutant strain can be prepared, for example, by subjecting the lactic acid bacterium to a known mutation treatment, adaptation by culturing the lactic acid bacterium according to the present embodiment, natural mutation, or the like.
  • lactic acid bacteria and mutants thereof according to this embodiment as described above can be used for various applications.
  • the microorganism of the present invention can efficiently decompose fibers such as cellulose, hemicellulose, and lignin, and is also suitable for decomposing organic substances containing a large amount of fibers such as bamboo and rice husk.
  • the lactic acid bacteria of this embodiment can be decomposed even at 40 to 50 ° C. (high temperature region), there is an advantage that the decomposition time of organic waste can be greatly shortened.
  • the microorganism of the present invention secretes an organic acid and can efficiently decompose the organic matter even in an acidic environment, and can produce effects such as intestinal regulation in the human body. Therefore, it can be used in the food field such as health foods and the medical field such as pharmaceuticals.
  • the lactic acid strain according to one aspect of the present invention is a novel lactic acid bacterium that has lactic acid-producing ability and has high-temperature resistance that does not die even at 60 ° C.
  • the lactic acid bacterium of the present invention can be used more efficiently and safely in various scenes. For example, when used for food processing, it is possible to process at a high temperature, thereby reducing the risk of food contamination. Moreover, in processes such as composting of organic waste using lactic acid bacteria, the waste decomposition process can be shortened by using the lactic acid bacteria of the present invention that grow even in a high temperature range. Therefore, according to the lactic acid bacterium of the present invention, it is considered that the application can be further expanded as compared with the conventional lactic acid bacterium.
  • Pediococcus acidilactici TT12-G5 strain NITE BP-1430
  • Pediococcus acidilactici TT12-PG2 strain NITE BP-1431
  • Lactobacillus fermentum TT12- 18h1 strain NITE BP-1448
  • Lactobacillus zeae TT12-S4 strain NITE BP-1433
  • Lactobacillus rhamnosus TT12-S1 strain NITE BP-1432
  • Lactobacillus casei TT12-18h4 strain NITEB
  • lactic acid bacteria having metabolic activity at 50 ° C. or higher are included in addition to the property of having a high temperature resistance that does not die even at 60 ° C. By having such characteristics, the effects of the present invention are more exhibited.
  • lactic acid bacteria examples include Pediococcus acidilactici TT12-G5 strain (NITE BP-1430), Pediococcus acidilactici TT12-PG2 strain (NITE BP-1431) or mutants thereof. Preferably it is selected.
  • a lactic acid bacterium that secretes an acid that dissolves calcium phosphate is preferable.
  • lactic acid bacteria Pediococcus acidilactici TT12-G5 strain (NITE BP-1430), Pediococcus acidilactici TT12-PG2 strain (NITE BP-1431), Lactobacillus fermentum TT12 -18h1 strain (NITE BP-1448), Lactobacillus zeae TT12-S4 strain (NITE BP-1433), Lactobacillus rhamnosus TT12-S1 strain (NITE BP-1432), Lactobacillus casei TT12-18h4 strain (NITE) BP-1429) or mutants thereof are preferably selected.
  • the lactic acid bacteria are preferably used for organic composting or food processing.
  • Test Example 1 Screening of lactic acid strains and HAP and protein resolution of selected strains 48 strains were isolated using lactic acid bacteria (Pediococcus and Lactobacillus) contained in Japanese compost as candidate strains. Isolation was performed by culturing using a medium for selecting lactic acid bacteria.
  • lactic acid bacteria Pediococcus and Lactobacillus
  • the isolated lactic acid bacteria were inherited and analyzed, and the first screening was performed. Specifically, identification was performed by comparing the base sequences of a highly specific region called ITS region (Internal Transcribed Spacer region) of filamentous fungi. Then, the following 8 strains having a high abundance ratio in the compost data used as the separation source were selected.
  • ITS region Internal Transcribed Spacer region
  • Pediococcus acidilactici TT12-G5 strain Pediococcus acidilactici TT12-PG2 strain, Lactobacillus fermentum TT12-18h1 strain, Lactobacillus zeae TT12-S4 strain, Lactobacillus rhamnosus TT12-S1 strain, Lactobacillus casei TT12-18h4 strain Bacillus coagulans BC1 strain Bacillus sumicil BS1 strain
  • a degradability test (halo test) was conducted for the purpose of observing the organic substance degradation characteristics due to the microbial function in the eight strains selected primarily.
  • a medium containing protein (skim milk, Wako Pure Chemical Industries) and hydroxyapatite (Apatite HAP, monoclinic, Wako Pure Chemical Industries) to observe the dissolution of bone fragments characteristic of composting reaction. Created.
  • the composition of the medium is as follows.
  • the proteolytic test was conducted as follows: 1. A microorganism is applied on a medium containing skim milk. 2. Microorganisms with protease activity degrade skim milk (mainly protein) and form a zona pellucida. 3. Proteolytic ability is evaluated from the size of the formed zona pellucida.
  • skim milk was replaced with apatite (calcium phosphate, the main component of bone), which is a white insoluble powder, to evaluate the ability of bone decomposition (dissolution by acid).
  • apatite calcium phosphate, the main component of bone
  • a sterilized absorption pad ( ⁇ 13 mm, Millipore) was placed on the prepared agar medium, and 50 ⁇ L of PBS buffer (pH 7.4) containing each candidate strain was soaked.
  • the absorbent pad was prepared so as to contain about 108 cells of microorganisms.
  • the culture was stationary at 40 ° C. or 50 ° C., and the degradation zone (halo) was observed after 24 hours.
  • TT12-G5 strain TT12-PG2 strain
  • TT12-18h1 strain TT12-S4 strain
  • TT12-S4 strain are shown as lactic acid strains showing high resolution.
  • TT12-S1 strain and TT12-18h4 strain were selected.
  • Test Example 2 Lactic acid and acetic acid production ability The lactic acid and acetic acid production ability of the 6 strains selected in Test Example 1 and related strains used for comparison were examined.
  • the related strains to be compared are related strains that can be distributed. Search for strains having a gene homology of 99% or more based on the 16rRNS gene sequence information of the strain according to the present Example, and the microorganisms depository organization (ATCC) , NBRC, JCM) were selected from three recent related strains (Pediococcus acidilactici NBRC 12218, Lactobacillus fermentum JCM 1137 and Lactobacillus reuteri JCM 8852). The distributed lyophilized strains were used after reconstitution with a designated medium.
  • ATCC microorganisms depository organization
  • a liquid medium was prepared using the following components excluding HAP and agar.
  • the culture medium was placed in a screw test tube, and the gas phase was sufficiently replaced with nitrogen gas and sealed.
  • Each microorganism grown on the agar medium was uniformly suspended in PBS buffer (pH 4.0), and inoculated into a screw-cap test tube so that the number of cells was 10 5 cells / ml.
  • the inside of the test tube was brought to a positive pressure (about 1.2 atmospheres) using nitrogen gas, and cultured with shaking (120 rpm) at 40 ° C. or 50 ° C. Sampling was performed after 24 hours of culture, and various organic acids and pH were measured.
  • sucrose and fructose which are saccharide main components in compost, were also examined for the ability to produce lactic acid and acetic acid. The test was carried out by substituting sucrose or fructose for each glucose.
  • the isolated strain TT12-18h1 which is closely related to Lactobacillus fermentum, also produced a large amount of lactic acid when fructose was used, and the production of acetic acid was also observed. It was shown that fructose is highly effective and sucrose has the ability to promote organic acid fermentation.
  • the isolated strain TT12-S1 which is closely related to Lactobacillus rhhamnosus, has a low ability to produce acetic acid, but has a very high ability to produce lactic acid when using fructose. A similar tendency is observed in the isolated strain TT12-S4, which is closely related to Lactobacillus zeae.
  • Isolated strain TT12-18h4 which is closely related to Lactobacillus casei, did not produce much organic acid with glucose and sucrose, and showed a high ability to produce lactic acid when using fructose.
  • sucrose utilization was shown only in the isolated strain TT12-18h1 closely related to Lactobacillus fermentum, and glucose utilization was shown in the isolated strain TT12-18h4 closely related to Lactobacillus casei.
  • all 6 strains showed fructose utilization, and the amount of lactic acid produced was high when using fructose.
  • fructose acetic acid is produced by lactic acid bacteria species that are predominantly present in compost, such as isolates TT12-G5 and TT12-PG2 and TT12-18h1, which are closely related to Lactobacillus fermentum. It is assumed that there is a high possibility that
  • the comparative example Pediococcus acidilactici NBRC 12218 (distributed strain) showed the ability to produce lactic acid and acetic acid at 40 ° C compared to the same Pediococcus acidilactici strains TT12-G5 and TT12-PG2, but completely at 50 ° C. Not shown.
  • Lactobacillus fermentum JCM 1137 and Lactobacillus reuteri JCM 8852 are also lactic acid than the strains of the examples. Or it was inferior to the acetic acid production ability.
  • Test Example 3 HAP resolution (bone component degradability test) The 6 strains selected in Test Example 1 and the related strains used for comparison were examined for HAP degradation performance.
  • the related strains to be compared include Pediococcus acidilactici NBRC12218 and Lactobacillus fermentum JCM1137 used in Test Example 2 as well as Lactobacillus plantarum subsp. plantarum 1923 NRBC 3070 was used.
  • the freeze-dried strain distributed from the microorganism depository was reconstituted with a designated medium and used for the test.
  • the bone component degradability test was performed in the same manner as in Test Example 1. The results are shown in Table 3 and FIGS.
  • Lactobacillus plantarum subsp. plantarum 1923 NRBC 3070 showed HAP degradability when glucose and fructose were used. However, as shown in FIG. 2, the formed halo (degradation zone) is not sufficiently transparent, so the HAP decomposing ability may be low. It was suggested.
  • Test Example 4 Bone Degradation Test TT12-G5 strain according to the present example and related lactic acid bacteria (Pediococcus acidilactici NBRC12218 and Lactobacillus plantarum subsp. Plantarum 1923 NRBC 3070 NRBC 3070) also used in Test Example 3 Bone resolution was compared.
  • the surface was washed with tap water, and then the pork bone was crushed using a bean grain size (1-2 cm square) as a guide. It was washed again with tap water to remove the inner cerebrospinal fluid and the like. It was boiled at 100 ° C. for 30 minutes to remove oil and protein. Washed with tap water and distilled water and dried. The dried bone fragments were autoclaved (121 ° C., 20 minutes) and dried. Each piece of bone was weighed using a sterilized petri dish and sterilized tweezers. Further, the weighing after the decomposition test was performed after the bone pieces were taken out of the test tube and sufficiently rinsed with tap water and sufficiently dried.
  • the TT12-G5 strain according to this example showed a degree of degradation of about 8%. Since all the microorganisms grew well, it was shown that the TT12-G5 strain has a higher ability to degrade bone than other microorganisms.
  • TT12-G5 which is a strain according to this example, showed a degree of degradation of about 8% as in the test at 40 ° C.
  • other microorganisms did not grow, and as a result, the degree of degradation was the same as that without microorganisms.
  • Pediococcus acidilactici NBRC 12218 which is a related species of isolated strain TT12-G5, showed some growth, but the degree of degradation was similar to that without microorganisms.
  • the present invention has wide industrial applicability in the technical field related to lactic acid bacteria and their use.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une nouvelle souche de lactobacille présentant d'excellentes propriétés de génération d'acide lactique et une excellente résistance à température élevée. Le lactobacille présente une résistance à température élevée telle qu'il n'est pas détruit même 60°C, etc., et d'excellents avantages dans de nombreuses applications.
PCT/JP2013/000098 2013-01-11 2013-01-11 Nouveau lactobacille WO2014108937A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/JP2013/000098 WO2014108937A1 (fr) 2013-01-11 2013-01-11 Nouveau lactobacille
JP2014556204A JP5988455B2 (ja) 2013-01-11 2013-01-11 新規乳酸菌

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2013/000098 WO2014108937A1 (fr) 2013-01-11 2013-01-11 Nouveau lactobacille

Publications (1)

Publication Number Publication Date
WO2014108937A1 true WO2014108937A1 (fr) 2014-07-17

Family

ID=51166618

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2013/000098 WO2014108937A1 (fr) 2013-01-11 2013-01-11 Nouveau lactobacille

Country Status (2)

Country Link
JP (1) JP5988455B2 (fr)
WO (1) WO2014108937A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017012157A (ja) * 2015-04-17 2017-01-19 リン， ジージューＪｈｙ−Ｊｈｕ ＬＩＮ 食品及び飼料調製のための高温耐性プロバイオティクス
WO2017132230A1 (fr) * 2016-01-25 2017-08-03 Novozymes A/S Procédé pour réduire la prolifération microbienne dans un couvoir à volailles
JP2018537972A (ja) * 2015-11-20 2018-12-27 ディーエスエム シノケム ファーマシューティカルズ ネザーランズ ビー．ヴイ． 廃棄物中の抗生物質の決定アッセイ
CN109294940A (zh) * 2018-09-04 2019-02-01 湖南肯基因科技有限公司 玉米乳杆菌诱变菌及高产乳酸的用途
TWI762280B (zh) * 2021-04-22 2022-04-21 許淙慶 可分解堆肥的製品

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109536409B (zh) * 2018-12-17 2022-03-11 吉林中粮生化有限公司 一种抗逆性高且可利用多种碳源的乳酸片球菌菌株及利用该菌株生产乳酸的方法
CN115011498A (zh) * 2021-03-05 2022-09-06 丰益(上海)生物技术研发中心有限公司 耐高温耐高糖的乳酸片球菌

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6474976A (en) * 1987-09-16 1989-03-20 Senjiyu Syuzo Kk Short-period 'sanpai' yeast culture using thermostable lactic acid bacteria
JP2003321282A (ja) * 2002-04-24 2003-11-11 Saburoku:Kk バイオ系多孔質セラミック及びその製造方法、並びに環境浄化材、環境浄化具
JP2003326296A (ja) * 2002-05-09 2003-11-18 Hino Saiseki:Kk 多孔質複合資材及びその製造方法
WO2007108764A2 (fr) * 2006-03-17 2007-09-27 Probac Ab Souches de lactobacillus thermostables

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03151856A (ja) * 1989-11-09 1991-06-28 Snow Brand Food Co Ltd 畜肉加工品の製造方法及びそれに使用するスターター製剤
JPH11207310A (ja) * 1998-01-26 1999-08-03 Denso Corp 生ゴミ処理方法及び生ゴミ処理装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6474976A (en) * 1987-09-16 1989-03-20 Senjiyu Syuzo Kk Short-period 'sanpai' yeast culture using thermostable lactic acid bacteria
JP2003321282A (ja) * 2002-04-24 2003-11-11 Saburoku:Kk バイオ系多孔質セラミック及びその製造方法、並びに環境浄化材、環境浄化具
JP2003326296A (ja) * 2002-05-09 2003-11-18 Hino Saiseki:Kk 多孔質複合資材及びその製造方法
WO2007108764A2 (fr) * 2006-03-17 2007-09-27 Probac Ab Souches de lactobacillus thermostables

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAMIREZ-CHAVARIN,N.L. ET AL.: "CHARACTERIZATION AND IDENTIFICATION OF THERMOTOLERANT LACTIC ACID BACTERIA ISOLATED FROM COOKED SAUSAGES AS BIOPROTECTIVE CULTURES", J MUSCLE FOODS, vol. 21, no. 3, 2010, pages 585 - 596 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017012157A (ja) * 2015-04-17 2017-01-19 リン， ジージューＪｈｙ−Ｊｈｕ ＬＩＮ 食品及び飼料調製のための高温耐性プロバイオティクス
JP2018537972A (ja) * 2015-11-20 2018-12-27 ディーエスエム シノケム ファーマシューティカルズ ネザーランズ ビー．ヴイ． 廃棄物中の抗生物質の決定アッセイ
WO2017132230A1 (fr) * 2016-01-25 2017-08-03 Novozymes A/S Procédé pour réduire la prolifération microbienne dans un couvoir à volailles
US11395479B2 (en) 2016-01-25 2022-07-26 Novozymes A/S Method to reduce microbial bloom in poultry hatchery
CN109294940A (zh) * 2018-09-04 2019-02-01 湖南肯基因科技有限公司 玉米乳杆菌诱变菌及高产乳酸的用途
CN109294940B (zh) * 2018-09-04 2021-06-29 湖南肯基因科技有限公司 玉米乳杆菌诱变菌及高产乳酸的用途
TWI762280B (zh) * 2021-04-22 2022-04-21 許淙慶 可分解堆肥的製品

Also Published As

Publication number Publication date
JPWO2014108937A1 (ja) 2017-01-19
JP5988455B2 (ja) 2016-09-07

Similar Documents

Publication Publication Date Title
JP5988455B2 (ja) 新規乳酸菌
Sakai et al. Open L-lactic acid fermentation of food refuse using thermophilic Bacillus coagulans and fluorescence in situ hybridization analysis of microflora
Nakasaki et al. Inoculation of Pichia kudriavzevii RB1 degrades the organic acids present in raw compost material and accelerates composting
Yamamoto et al. Vulgatibacter incomptus gen. nov., sp. nov. and Labilithrix luteola gen. nov., sp. nov., two myxobacteria isolated from soil in Yakushima Island, and the description of Vulgatibacteraceae fam. nov., Labilitrichaceae fam. nov. and Anaeromyxobacteraceae fam. nov.
KR20170021002A (ko) 음식물 분해를 위한 미생물제제
Lee et al. Weissella jogaejeotgali sp. nov., isolated from jogae jeotgal, a traditional Korean fermented seafood
KR101642321B1 (ko) 음식물 쓰레기 분해 소멸 미생물과 이를 포함하는 분해소멸용 조성물 및 이를 이용한 음식물 쓰레기의 소멸 방법
US9896706B2 (en) Method for producing β-glucan
Tamang Native microorganisms in the fermentation of kinema
Qiu et al. Characterization of garlic endophytes isolated from the black garlic processing
Rapsang et al. Identification of Lactobacillus pobuzihii from tungtap: a traditionally fermented fish food, and analysis of its bacteriocinogenic potential
Tongpim et al. Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition
KR20160003587A (ko) 음식물 쓰레기 분해 소멸 미생물과 이를 포함하는 분해소멸용 조성물 및 이를 이용한 음식물 쓰레기의 소멸 방법
KR101920557B1 (ko) 음식물 쓰레기 분해용 혼합균주 및 이를 이용한 음식물 쓰레기 처리 방법
Choi et al. Characterization of Bacillus mojavensis KJS-3 for industrial applications
JP5445822B2 (ja) 有機廃棄物分解作用を示す微生物、微生物組成物、有機廃棄物の分解方法および堆肥の製造方法
Bano et al. A novel, thermotolerant, extracellular PHB depolymerase producer Paenibacillus alvei PHB28 for bioremediation of biodegradable plastics
JP2008199938A (ja) バチルス属微生物を用いた有機系廃棄物の処理方法
KR102402633B1 (ko) 바실러스균과 유산균의 혼합 균주의 성장을 증진시키기 위한 최적 배지 조성물 및 이의 용도
Bashandy et al. Using fermentation waste of ethanol‐producing yeast for bacterial riboflavin production and recycling of spent bacterial mass for enhancing the growth of oily plants
KR101529832B1 (ko) 음식물쓰레기 처리용 혼합 균주 및 이를 이용한 음식물쓰레기 처리방법
JP5445821B2 (ja) 有機廃棄物分解作用を示す微生物、微生物組成物、有機廃棄物の分解方法および堆肥の製造方法
JP2008142042A (ja) 混合微生物，製剤および油脂含有物質の処理方法
JP4876247B2 (ja) ペニシリウム属に属する新規微生物
Saranya et al. Isolation and characterization of cholesterol degrading bacteria from soap and vegetable oil industrial waste

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13871155

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2014556204

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13871155

Country of ref document: EP

Kind code of ref document: A1