WO2014050605A1 - Récipient de centrifugation - Google Patents

Récipient de centrifugation Download PDF

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Publication number
WO2014050605A1
WO2014050605A1 PCT/JP2013/074785 JP2013074785W WO2014050605A1 WO 2014050605 A1 WO2014050605 A1 WO 2014050605A1 JP 2013074785 W JP2013074785 W JP 2013074785W WO 2014050605 A1 WO2014050605 A1 WO 2014050605A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
specimen
reservoir
centrifuge
central axis
Prior art date
Application number
PCT/JP2013/074785
Other languages
English (en)
Japanese (ja)
Inventor
朋宣 西尾
Original Assignee
富士フイルム株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 富士フイルム株式会社 filed Critical 富士フイルム株式会社
Publication of WO2014050605A1 publication Critical patent/WO2014050605A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B04B1/02—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles without inserted separating walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Definitions

  • the present invention relates to a centrifuge container for centrifuging a sample component in a container by being rotated about a central axis of the container.
  • This centrifuge device stores a specimen such as blood inside a centrifuge container, and centrifuges the components of the specimen in the container by rotating the centrifuge container around the central axis as a rotation axis. .
  • the centrifuge container used in the above-described centrifuge includes a reservoir that stores the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body. It is inclined so as to become higher from the center toward the outer periphery, and has an annular trap portion having a volume for storing a centrifugally separated component having a high specific gravity.
  • a separation gel having an intermediate specific gravity between the components to be separated in the specimen is arranged, and when the container is rotated while the specimen is injected into the storage part, only the component having a higher specific gravity than the separation gel is provided. Moves to the outer peripheral side of the separation gel in the trap portion, and the component having a specific gravity lower than that of the separation gel remains inside the separation gel, whereby the components of the specimen can be separated.
  • the specific gravity is usually lower than that of the separation gel and the inner side of the separation gel.
  • the component staying at the bottom is stored near the lower surface center of the storage part, but in rare cases, it does not come down near the lower surface center of the storage part while sticking to the outer periphery of the storage part.
  • the amount of specimen processed at one time in the centrifuge container as described above is not so high as 600 to 800 ⁇ L, so if the components extracted by centrifugation remain stuck in the reservoir, the recoverable components will decrease. This causes the problem that the necessary amount cannot be recovered.
  • Patent Document 1 In order to solve such a problem, in Patent Document 1, the hydrophilic region and the hydrophobic region are arranged in an appropriate shape in the storage portion of the centrifuge container, and components extracted by centrifugation are stuck in the storage portion. It is devised not to leave.
  • the present invention has been made in view of the above problems, and causes a cost increase in a centrifuge container for centrifuging a component of a specimen in a container by being rotated about the central axis of the container.
  • the object of the present invention is to provide a centrifuge container configured such that components extracted by centrifugation do not remain stuck in the reservoir.
  • the centrifuge container of the present invention includes a reservoir for storing a specimen inside the container, and the centrifuge container for centrifuging the components of the specimen in the reservoir by being rotated around the central axis of the container
  • a three-dimensional structure including a corner portion extending in the outer peripheral direction from the central axis at the inner surface position of the reservoir that straddles the boundary surface between the specimen existing on the outer peripheral side of the container and the air on the central axis side when the container rotates. It is formed.
  • ⁇ a three-dimensional structure is formed at the inner surface position of the reservoir that straddles the boundary surface between the specimen existing on the outer peripheral side of the container and the air on the central axis side during rotation of the container '' This means that the three-dimensional structure is formed so that the whole three-dimensional structure is not covered by the stuck specimen.
  • “extending in the outer circumferential direction from the central axis” means having a component extending in the radial direction around the central axis, and does not necessarily have to coincide with the radial direction. It may be inclined.
  • a three-dimensional structure may be formed only in a partial region from the central axis to the outer periphery.
  • the container is provided with an opening for injecting the specimen into the reservoir, and a three-dimensional structure is formed on at least the surface (usually the upper surface) provided with the opening among the inner surfaces of the reservoir. It is preferable that
  • a three-dimensional structure in which a plurality of grooves and / or protrusions are radially arranged from the central axis toward the outer peripheral direction may be formed on any inner surface of the storage unit.
  • the container extends from the central axis in the outer peripheral direction to the inner surface position of the reservoir that straddles the boundary surface between the specimen existing on the outer peripheral side of the container and the air on the central axis side when the container rotates.
  • a three-dimensional structure including corners is formed, such a three-dimensional structure is only necessary to adjust the shape of the mold when the container is formed by injection molding or the like. It is possible to prevent the extracted components from sticking in the reservoir.
  • the container is provided with an opening for injecting the specimen into the reservoir, and a three-dimensional structure is formed on at least the surface (usually the upper surface) provided with the opening among the inner surfaces of the reservoir.
  • the plurality of grooves and / or protrusions are radially arranged from the central axis toward the outer peripheral direction
  • the plurality of grooves and / or Since air can be introduced between the inner surface of the reservoir and the specimen attached to the inner surface by the protrusions, the specimen can be more reliably separated from the inner surface.
  • the top view of the container for centrifugation of one embodiment of the present invention Sectional view of the above centrifuge container (sectional view taken along line II-II in FIG. 1)
  • Sectional view of the upper member of the centrifuge container (sectional view taken along line IV-IV in FIG. 3)
  • Sectional drawing which shows the other aspect of the upper member of the container for centrifugation of this invention.
  • Schematic configuration diagram of a centrifuge using the above centrifuge container The figure which shows the state at the time of the sample injection
  • FIG. 1 is a top view of a centrifuge container according to an embodiment of the present invention
  • FIG. 2 is a cross-sectional view of the centrifuge container (cross-sectional view taken along the line II-II in FIG. 1)
  • FIG. 4 is a cross-sectional view of the upper member of the centrifuge container (IV-IV line cross-sectional view of FIG. 3)
  • FIG. 5 is a view of the centrifuge container of the present invention. It is sectional drawing which shows the other aspect of an upper member.
  • the centrifuge container 1 includes a storage unit 11 that stores a specimen inside a container body 10 that is substantially cylindrical in appearance, and injects the specimen into the storage unit 11 on the upper surface of the container body 10.
  • the opening 12 is provided.
  • the container body 10 is formed by fitting and fixing the upper member 10a and the lower member 10b, and the lower surface of the storage portion 11 formed between the upper member 10a and the lower member 10b is the outer periphery from the center.
  • An annular trap portion 13 having a volume for storing a high specific gravity component centrifuged from the specimen is formed on the outer peripheral portion of the storage portion 11.
  • a separation gel 14 having an intermediate specific gravity between components to be separated in the specimen is disposed.
  • a gel having a specific gravity intermediate between the blood cell component and the plasma component may be used as the separation gel 14.
  • the inner upper surface (upper member 10a) of the reservoir 11 has a boundary surface between the specimen 32 present on the outer peripheral side of the container and the air on the central axis side when the container rotates.
  • a three-dimensional structure (groove 20) including corner portions extending in the outer peripheral direction from the central axis C is formed at the straddling inner surface position. More specifically, a three-dimensional structure is formed in which a plurality of grooves 20 are radially arranged from the central axis toward the outer peripheral direction. And each groove
  • channel 20 has comprised the cross-sectional shape which has a square-shaped corner
  • the corner portion is not limited to a right angle as long as the corner is not rounded, and may be an acute angle or an obtuse angle.
  • the groove 20 may be a three-dimensional structure having a width and a depth of about 0.1 mm or more and including a corner portion.
  • the surface tension acts to move away from the corners, so that air enters the groove, and the air between the inner upper surface of the reservoir 11 and the specimen (example of plasma component 32 described later) stuck on the inner upper surface. Since the air enters between the inner upper surface of the reservoir 11 and the specimen (example of a plasma component 32 described later) attached to the inner upper surface of the reservoir 11 after the passage portion 20a is formed, the specimen is separated from the inner upper surface. be able to.
  • Such a three-dimensional structure does not cause an increase in cost because it is only necessary to adjust the shape of the mold when the upper member 10a is formed by injection molding or the like.
  • the component staying on the inner side of the separation gel 14 on the upper surface side that becomes the main resistance when descending near the center of the lower surface of the storage portion 11 Can eliminate sticking of extracted components.
  • the plurality of grooves 20 have a three-dimensional structure radially arranged from the central axis toward the outer peripheral direction, the plurality of grooves 20 are provided between the inner upper surface of the storage unit 11 and the specimen attached to the inner upper surface. Can put in air.
  • the centrifuge container 1 more reliably separates the specimen from the inner upper surface of the storage unit 11 without causing an increase in cost, and the components extracted by the centrifugation are stored in the storage unit 11. You can keep it from sticking.
  • a protrusion 21 instead of a groove
  • an air passage 21 a is formed between the inner upper surface of the reservoir 11 and the specimen (example of a plasma component 32 described later) attached to the inner upper surface, and the inner upper surface and the inner upper surface of the reservoir 11 are formed. Since air can be introduced between the attached specimen (the plasma component 32 described below is exemplified), the same effect as described above can be exhibited.
  • variety and height of the protrusion 21, what is necessary is just about 0.1 mm or more like a groove
  • channel or a protrusion it arrange
  • the grooves and protrusions may be combined.
  • FIG. 6 is a schematic configuration diagram of a centrifuge using the centrifuge container.
  • the centrifuge 50 includes a housing 52 for housing the centrifuge container 1 inside the housing 51.
  • the accommodating portion 52 is provided with a holding portion 53 for holding the centrifuge container 1, and this holding portion 53 is rotatably supported by a rotation mechanism (not shown), and the centrifuge held by the holding portion 53.
  • the center axis C of the separation container 1 is configured to coincide with the rotation axis of the rotation mechanism.
  • FIG. 7 is a diagram showing a state of the centrifuge container when the sample is charged
  • FIG. 8 is a diagram showing a centrifuge state of the centrifuge container
  • FIG. 9 is a centrifuge state of the centrifuge container.
  • FIG. Here, a case where blood is used as a sample is shown as an example.
  • blood 30 is injected into the reservoir 11 of the centrifuge container 1. Specifically, blood 30 is injected from the opening 12 provided on the upper surface of the container body 10 by a pipette, a syringe or the like.
  • the centrifuge container 1 into which the blood 30 has been injected is accommodated in the accommodating part 52 of the centrifuge 50 and rotated about the central axis C of the centrifuge container 1 as a rotation axis. Then, as shown in FIG. 8, only the blood cell component 31 having a specific gravity higher than that of the separation gel 14 moves to the outer peripheral side of the separation gel 14 in the trap portion 13, and the plasma component having a specific gravity lower than that of the separation gel 14. 32 sticks to the inner peripheral side of the separation gel 14.
  • the blood cell component 31 on the outer peripheral side of the separation gel 14 remains trapped in the trap portion 13 and is more internal than the separation gel 14. Only the plasma component 32 on the circumferential side descends and is stored near the center of the lower surface of the storage unit 11. At this time, since the centrifuge container 1 according to the present embodiment forms the above-described three-dimensional structure on the inner upper surface of the reservoir 11, the plasma component 32 extracted by centrifugation is stretched in the reservoir 11. You can keep it from sticking. Thereby, only the plasma component 32 can be extracted efficiently.
  • the outer diameter of the trap portion 13 is 25 mm
  • the inner diameter is 20 mm
  • the height is 1 mm.
  • the inclination angle ⁇ a of the inner upper surface (upper member 10a) and the inclination angle ⁇ b of the inner lower surface (lower member 10b) of the storage part 11 are both formed at 30 ° to 60 °.
  • the height from the lower end of the storage part 11 to an upper end is 15 mm.
  • the groove 20 is formed so as to extend from the inclined upper end of the upper member 10 a to the outer peripheral end of the trap portion 13.
  • a 400 ⁇ L separation gel 14 is arranged in advance in the trap section 13, and 600 to 800 ⁇ L of sample is supplied into the storage section 11. Accordingly, a substance having a volume of 1000 to 1200 ⁇ L rotates in the storage unit 11 during centrifugation.
  • the groove 20 is formed so as to extend from the inclined upper end of the upper member 10a to the outer peripheral end of the trap portion 13. Since the groove 20 straddles the boundary surface between the plasma component 32 existing on the outer peripheral side of the container and the air on the central axis side when the container is rotated, the plasma component 32 is formed on the inner upper surface of the reservoir 11 after the rotation is stopped. Even when it is attached, air can enter between the inner upper surface of the reservoir 11 and the plasma component 32 attached to the inner upper surface, and the specimen can be separated from the inner upper surface and lowered to the vicinity of the center of the lower surface of the reservoir. .
  • the centrifuge is provided with a mechanism that applies a light impact to the centrifuge container 1 after centrifugation, the sample is more reliably separated from the inner upper surface of the reservoir 11 and extracted by centrifugation. It is possible to prevent the component that has been left from sticking in the reservoir 11.
  • a mechanism any mechanism such as striking the holding portion 53 with a light hammer or swinging the holding portion 53 using a cam may be used.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • External Artificial Organs (AREA)

Abstract

L'invention concerne un récipient de centrifugation amené à tourner autour de son axe central, permettant ainsi de centrifuger des composants d'un échantillon dans le récipient, les composants extraits par centrifugation étant empêchés d'adhérer à l'intérieur d'une partie de réservoir sans dépense additionnelle. L'invention concerne un récipient de centrifugation (1) comportant une partie réservoir (11) pour retenir un échantillon à l'intérieur d'un corps de récipient (10) ayant un aspect extérieur sensiblement cylindrique, la surface supérieure du corps de récipient (10) comportant une ouverture (12) à travers laquelle l'échantillon est introduit dans la partie réservoir (11), une structure tridimensionnelle (un canal (20)) comprenant une partie inclinée s'étendant à partir de l'axe central (C) dans la direction circonférentielle externe étant formée à une position sur la surface interne de la partie réservoir le long de l'interface entre l'échantillon (32), qui est présent sur le côté circonférentiel externe du récipient, et l'air qui est présent vers l'axe central lorsque le récipient tourne.
PCT/JP2013/074785 2012-09-28 2013-09-13 Récipient de centrifugation WO2014050605A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012216075A JP2016000370A (ja) 2012-09-28 2012-09-28 遠心分離用容器
JP2012-216075 2012-09-28

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WO2014050605A1 true WO2014050605A1 (fr) 2014-04-03

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (ja) * 2013-03-29 2014-11-06 富士フイルム株式会社 遠心分離用容器および遠心分離装置並びにそれらを用いた遠心分離方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (fr) * 1972-11-03 1974-12-23
JPS62273065A (ja) * 1986-05-12 1987-11-27 マイルス・ラボラトリ−ズ・インコ−ポレ−テッド 液体中に懸濁した微細な固体粒物質を分別・分離するための装置
JP2001239185A (ja) * 2000-03-02 2001-09-04 Arkray Inc 遠心分離用容器

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (fr) * 1972-11-03 1974-12-23
JPS62273065A (ja) * 1986-05-12 1987-11-27 マイルス・ラボラトリ−ズ・インコ−ポレ−テッド 液体中に懸濁した微細な固体粒物質を分別・分離するための装置
JP2001239185A (ja) * 2000-03-02 2001-09-04 Arkray Inc 遠心分離用容器

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (ja) * 2013-03-29 2014-11-06 富士フイルム株式会社 遠心分離用容器および遠心分離装置並びにそれらを用いた遠心分離方法

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JP2016000370A (ja) 2016-01-07

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