WO2014050021A1 - Récipient de centrifugation - Google Patents

Récipient de centrifugation Download PDF

Info

Publication number
WO2014050021A1
WO2014050021A1 PCT/JP2013/005498 JP2013005498W WO2014050021A1 WO 2014050021 A1 WO2014050021 A1 WO 2014050021A1 JP 2013005498 W JP2013005498 W JP 2013005498W WO 2014050021 A1 WO2014050021 A1 WO 2014050021A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
centrifugation
opening
centrifuge
sample
Prior art date
Application number
PCT/JP2013/005498
Other languages
English (en)
Japanese (ja)
Inventor
朋宣 西尾
Original Assignee
富士フイルム株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 富士フイルム株式会社 filed Critical 富士フイルム株式会社
Publication of WO2014050021A1 publication Critical patent/WO2014050021A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B04B1/02—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles without inserted separating walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs

Definitions

  • the present invention relates to a centrifuge container for centrifuging a sample component in a container by being rotated about a central axis of the container.
  • This centrifuge device stores a specimen such as blood inside a centrifuge container, and centrifuges the components of the specimen in the container by rotating the centrifuge container around the central axis as a rotation axis. .
  • the centrifuge container used in the above-described centrifuge includes a reservoir that stores the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body. It is inclined so as to become higher from the center toward the outer periphery, and has an annular trap portion having a volume for storing a centrifugally separated component having a high specific gravity.
  • a separation gel having an intermediate specific gravity between the components to be separated in the specimen is arranged, and when the container is rotated while the specimen is injected into the storage part, only the component having a higher specific gravity than the separation gel is provided. Moves to the outer peripheral side of the separation gel in the trap portion, and the component having a specific gravity lower than that of the separation gel remains inside the separation gel, whereby the components of the specimen can be separated.
  • the sample in the reservoir may be aerosolized and scattered from the opening to the outside.
  • the present invention has been made in view of the above problems, and in a centrifuge container for centrifuging components of a specimen in a container by being rotated about the central axis of the container, the specimen is centrifuged at the time of centrifugation.
  • Centrifuge that can automatically dispense extracted components from a centrifuge container after centrifuging without using a device that automatically opens the lid and prevents the aerosol from being aerosolized.
  • the object is to provide a separation container.
  • the centrifuge container of the present invention includes a reservoir for storing the specimen inside the container, and an opening for injecting the specimen into the reservoir is provided in the container, and the container is rotated about the central axis of the container as a rotation axis.
  • a centrifuge container for centrifuging the components of the specimen in the reservoir further comprising an attachable sealing lid that closes the opening, the sealing lid being attached to the opening by a dispensing tip. It has a sealing member that can be penetrated and that can collect a sample in the reservoir by a penetrating dispensing tip.
  • the opening is preferably on the central axis of the container.
  • the sealing member may be a flexible sheet in which a septum or a pinhole is formed.
  • the centrifuge container of the present invention further includes a sealable lid that closes the opening and that can be attached.
  • a sealable lid that closes the opening and that can be attached.
  • the sealable lid When the sealable lid is attached to the opening, it can be penetrated by the dispensing tip, and dispensed through. Since the chip has a seal member that can collect the specimen in the reservoir, it can prevent the specimen from being aerosolized and scattered outside, and automatically extract components from the centrifuge container after centrifugation.
  • the centrifuge container of the present invention if the opening is arranged on the central axis of the container, the centrifugal force applied to the sealing member of the sealing lid at the time of centrifugation is reduced. The risk of damage is reduced. Further, if the strength of the seal member is lowered, the seal member can be penetrated by a dispensing tip with a small pressing force, and therefore the operation during automatic dispensing can be facilitated.
  • the surface of the seal member is arranged so as to extend in a direction substantially perpendicular to the central axis of the container, no shear force is applied to the seal member during centrifugation, and only a tensile force in the outer peripheral direction is applied. Similarly to the above, even if the strength of the seal member is lowered, the possibility that the seal member is damaged is lowered.
  • the sealing lid can be reused.
  • FIG. 1 The top view of the container for centrifugation of one embodiment of the present invention
  • Sectional view of the above centrifuge container (sectional view taken along line II-II in FIG. 1) Schematic configuration diagram of a centrifuge using the above centrifuge container
  • the figure which shows the state at the time of centrifugation in the said container for centrifugation The figure which shows the state after centrifugation in the said container for centrifugation
  • FIG. 1 is a top view of a centrifuge container according to an embodiment of the present invention
  • FIG. 2 is a cross-sectional view of the centrifuge container (a cross-sectional view taken along the line II-II in FIG. 1).
  • the centrifuge container 1 includes a storage unit 11 that stores a specimen inside a container body 10 that is substantially cylindrical in appearance, and injects the specimen into the storage unit 11 on the upper surface of the container body 10.
  • the opening 12 is provided.
  • the container body 10 is formed by fitting and fixing the upper member 10a and the lower member 10b, and the lower surface of the storage portion 11 formed between the upper member 10a and the lower member 10b is the outer periphery from the center.
  • An annular trap portion 13 having a volume for storing a high specific gravity component centrifuged from the specimen is formed on the outer peripheral portion of the storage portion 11.
  • a separation gel 14 having an intermediate specific gravity between components to be separated in the specimen is disposed.
  • a gel having a specific gravity intermediate between the blood cell component and the plasma component may be used as the separation gel 14.
  • a detachable sealing lid 20 that closes the opening is attached to the opening 12.
  • the sealing lid 20 has a chip insertion port 21 that communicates with the storage part 11 of the container body 10 and the outside when mounted on the opening 12.
  • the tip insertion port 21 is provided with a seal member 22 that can be penetrated by a dispensing tip.
  • the chip insertion port 21 is blocked by the seal member 22, and the storage portion 11 is sealed when the sealing lid 20 is attached to the opening 12.
  • the seal member 22 may be provided on either the upper side or the lower side of the chip insertion port 21. However, the seal member 22 is provided on the lower side of the chip insertion port 21 when the user attaches the sealing lid 20 to the opening 12. Since the user's finger is less likely to touch the seal member 22, it is possible to prevent the seal member 22 from being accidentally damaged.
  • the opening 12 is disposed on the central axis C of the container body 10.
  • the centrifugal force applied to the seal member 22 of the sealing lid 20 at the time of centrifugation is reduced, so that the risk of damage to the seal member 22 is reduced even if the strength of the seal member 22 is reduced.
  • the surface of the seal member 22 is arranged so as to extend in a direction substantially orthogonal to the central axis of the centrifuge container, whereby no shear force is applied to the seal member 22 during centrifugal separation, and a tensile force in the outer circumferential direction.
  • the seal member 22 can be penetrated by a dispensing tip with a small pressing force, and thus the operation during automatic dispensing can be facilitated.
  • FIG. 3 is a schematic configuration diagram of a centrifuge using the centrifuge container.
  • the centrifuge 50 includes a housing 52 for housing the centrifuge container 1 inside the housing 51.
  • the accommodating portion 52 is provided with a holding portion 53 for holding the centrifuge container 1, and this holding portion 53 is rotatably supported by a rotation mechanism (not shown), and the centrifuge held by the holding portion 53.
  • the center axis C of the separation container 1 is configured to coincide with the rotation axis of the rotation mechanism.
  • FIG. 4 is a diagram showing a state of the centrifuge container when the sample is charged
  • FIG. 5 is a diagram showing a centrifuge state of the centrifuge container
  • FIG. 6 is a centrifuge state of the centrifuge container.
  • FIG. Here, a case where blood is used as a sample is shown as an example.
  • blood 30 is injected into the storage part 11 of the centrifuge container 1. Specifically, blood 30 is injected from the opening 12 provided on the upper surface of the container body 10 by a pipette, a syringe or the like. After the blood 30 is injected, the sealing lid 20 is attached to the opening 12.
  • the centrifuge container 1 into which the blood 30 has been injected is accommodated in the accommodating part 52 of the centrifuge 50 and rotated about the central axis C of the centrifuge container 1 as a rotation axis. Then, as shown in FIG. 5, only the blood cell component 31 having a specific gravity higher than that of the separation gel 14 moves to the outer peripheral side of the separation gel 14 in the trap portion 13, and the plasma component having a specific gravity lower than that of the separation gel 14. 32 sticks to the inner peripheral side of the separation gel 14.
  • the blood 30 in the reservoir 11 may be aerosolized and scattered from the opening 12 to the outside.
  • the sealing lid 20 since the sealing lid 20 is attached to the opening 12, the aerosolized blood It is possible to prevent 30 from being scattered outside the container.
  • the blood cell component 31 on the outer peripheral side of the separation gel 14 remains trapped in the trap part 13 and is more internal than the separation gel 14. Only the plasma component 32 on the circumferential side descends and is stored near the center of the lower surface of the storage unit 11. Thereby, only the plasma component 32 can be extracted.
  • the plasma component 32 when the plasma component 32 is automatically dispensed from the centrifuge container 1 after centrifugation, it is not necessary to remove the sealing lid 20, and the dispensing tip 40 is directly put into the chip insertion port 21 of the sealing lid 20. Since the plasma component 32 in the reservoir 11 can be dispensed by being inserted and penetrating the seal member 22, the plasma component 32 can be automatically dispensed without using a device that automatically opens the lid. Can correspond to dispensing.
  • the sealing member is not a sheet-like member that cannot be repaired once penetrated as described above, but the septum or pinhole is formed in the flexible sheet, the sealing lid is re-opened. It can be used.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Abstract

L'invention vise à procurer un récipient de centrifugation pour la centrifugation de composants d'un échantillon dans un récipient par rotation avec l'axe central du récipient comme axe de rotation, dans lequel l'échantillon est empêché d'être mis sous forme d'aérosol pendant la centrifugation et d'être dispersé à l'extérieur, et qui assure une distribution automatique du composant d'extraction à partir du récipient de centrifugation après une centrifugation sans utiliser des dispositifs similaires à un dispositif qui ouvre automatiquement le couvercle. A cet effet, l'invention porte sur un récipient de centrifugation (1), lequel récipient comporte une partie de stockage (11) pour stocker un échantillon dans un corps principal de récipient (10) ayant un aspect sensiblement cylindrique, et dans lequel la surface supérieure du corps principal de récipient (10) comporte une ouverture (12) pour verser l'échantillon dans la partie de stockage (11). Le récipient de centrifugation (1) comporte de plus un couvercle d'étanchéité pouvant être attaché (20) qui bouche l'ouverture (12). Le couvercle d'étanchéité (20) a un élément d'étanchéité (22), qui, quand le couvercle d'étanchéité (20) est attaché à l'ouverture (12), peut être pénétré par une pointe de distribution, et permet à l'échantillon à l'intérieur de la partie de stockage (11) d'être extrait par la pointe de distribution qui a pénétré à travers l'élément d'étanchéité (22).
PCT/JP2013/005498 2012-09-28 2013-09-18 Récipient de centrifugation WO2014050021A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012215662 2012-09-28
JP2012-215662 2012-09-28

Publications (1)

Publication Number Publication Date
WO2014050021A1 true WO2014050021A1 (fr) 2014-04-03

Family

ID=50387464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2013/005498 WO2014050021A1 (fr) 2012-09-28 2013-09-18 Récipient de centrifugation

Country Status (1)

Country Link
WO (1) WO2014050021A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (ja) * 2013-03-29 2014-11-06 富士フイルム株式会社 遠心分離用容器および遠心分離装置並びにそれらを用いた遠心分離方法
WO2017100798A1 (fr) 2015-12-11 2017-06-15 Siemens Healthcare Diagnostics Inc. Contenant d'échantillon et procédé de séparation de sérum ou plasma et de sang total
EP3414015A4 (fr) * 2015-06-23 2020-07-22 Karilahti, Mika Centrifugeuse et système pour un échantillon de fluide corporel
US12025629B2 (en) 2023-04-14 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (fr) * 1972-11-03 1974-12-23
JPH10512960A (ja) * 1995-10-03 1998-12-08 ベックマン インスツルメンツ インコーポレーテッド 軸線回り回転の血液分離装置及び方法
JP2000117150A (ja) * 1998-10-16 2000-04-25 Kdk Corp 遠心分離方法及びそれに用いる遠心容器
JP2004053301A (ja) * 2002-07-17 2004-02-19 Yasui Kikai Kk 破砕試料の処理方法及びそれに用いる破砕容器
JP2010517022A (ja) * 2007-01-25 2010-05-20 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 血液サンプルの液状成分を分離する装置および方法、ならびにそのような装置を備える分析装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (fr) * 1972-11-03 1974-12-23
JPH10512960A (ja) * 1995-10-03 1998-12-08 ベックマン インスツルメンツ インコーポレーテッド 軸線回り回転の血液分離装置及び方法
JP2000117150A (ja) * 1998-10-16 2000-04-25 Kdk Corp 遠心分離方法及びそれに用いる遠心容器
JP2004053301A (ja) * 2002-07-17 2004-02-19 Yasui Kikai Kk 破砕試料の処理方法及びそれに用いる破砕容器
JP2010517022A (ja) * 2007-01-25 2010-05-20 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 血液サンプルの液状成分を分離する装置および方法、ならびにそのような装置を備える分析装置

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (ja) * 2013-03-29 2014-11-06 富士フイルム株式会社 遠心分離用容器および遠心分離装置並びにそれらを用いた遠心分離方法
EP3414015A4 (fr) * 2015-06-23 2020-07-22 Karilahti, Mika Centrifugeuse et système pour un échantillon de fluide corporel
WO2017100798A1 (fr) 2015-12-11 2017-06-15 Siemens Healthcare Diagnostics Inc. Contenant d'échantillon et procédé de séparation de sérum ou plasma et de sang total
CN108601565A (zh) * 2015-12-11 2018-09-28 西门子医疗保健诊断公司 用于从全血分离血清或血浆的样品容器和方法
EP3386391A4 (fr) * 2015-12-11 2018-10-17 Siemens Healthcare Diagnostics Inc. Contenant d'échantillon et procédé de séparation de sérum ou plasma et de sang total
US10870110B2 (en) 2015-12-11 2020-12-22 Babson Diagnostics, Inc. Specimen container and centrifugation method for separating serum or plasma from whole blood therewith
CN108601565B (zh) * 2015-12-11 2021-09-07 巴布森诊断公司 用于从全血分离血清或血浆的样品容器和方法
US11697114B2 (en) 2015-12-11 2023-07-11 Babson Diagnostics, Inc. Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells
US12025629B2 (en) 2023-04-14 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

Similar Documents

Publication Publication Date Title
KR101286442B1 (ko) 용기용의 비틀림 절단 개봉기구
US10870110B2 (en) Specimen container and centrifugation method for separating serum or plasma from whole blood therewith
JP5021323B2 (ja) 検査用容器
US9081022B2 (en) Centrifuge rotor for separation and processing of complex fluids
RU2605173C2 (ru) Устройство закупоривания и емкость, оборудованная таким устройством
WO2014050021A1 (fr) Récipient de centrifugation
JP2018505005A (ja) 複合液体バッグシステムホルダ
KR102158645B1 (ko) 액체 포장 용기
US9662664B2 (en) Centrifuge with automatically opening rotor cover
CN105102956B (zh) 离心涂抹装置及密闭旋转容器
EP2431095A2 (fr) Tube à essai séparable pour séparateur centrifuge
EP2979764B1 (fr) Contenant de séparation centrifuge, dispositif de séparation centrifuge et procédé de séparation centrifuge au moyen dudit contenant et dudit dispositif
WO2015080918A1 (fr) Système de poche de sang et porte-tube
TW201517877A (zh) 特別用於血樣之收集組件
JP2014502353A (ja) サンプリングツールで採取したサンプルを保存及び処理するためのサンプルコンテナ
JP6180233B2 (ja) 注出用収納容器
EP3202332B1 (fr) Récipient de collecte d'échantillons de matières fécales
EP2985588A1 (fr) Dispositif d'adhérence par centrifugation et récipient étanche en rotation
EP2891453B1 (fr) Instrument de prélèvement de sang, et système de sac de sang
WO2014050605A1 (fr) Récipient de centrifugation
JP2014198281A (ja) 遠心分離用容器および遠心分離装置並びにそれらを用いた遠心分離方法
JP3062082B2 (ja) 液体の遠心分離用沈殿管
JP6599254B2 (ja) 遠心分離用ロータ及び遠心分離装置
JP2014065019A (ja) 遠心分離用容器
US20220331818A1 (en) Disposable Centrifuge Pot

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13841620

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13841620

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: JP