Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
  • C12N9/10—Transferases (2.)
  • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
  • C12N9/1241—Nucleotidyltransferases (2.7.7)
  • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
  • A23L29/06—Enzymes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/17—Amino acids, peptides or proteins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
  • A61K38/45—Transferases (2)
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
  • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
  • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
  • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
  • A61K8/66—Enzymes
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/475—Growth factors; Growth regulators
  • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
  • C07K16/46—Hybrid immunoglobulins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/10—General cosmetic use
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
  • A61K2800/86—Products or compounds obtained by genetic engineering
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2319/00—Fusion polypeptide
  • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
  • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2319/00—Fusion polypeptide
  • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
  • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2319/00—Fusion polypeptide
  • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Y—ENZYMES
  • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
  • C12Y207/07—Nucleotidyltransferases (2.7.7)
  • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Definitions

• the present specification relates to a cell-penetrating peptide derived from telomerase, a conjugate of the cell-penetrating peptide and an active ingredient, and a supernatant including the conjugate.
• nucleic acid proteins or nanoparticles have great potential as therapeutic materials at the molecular level, but their use is limited to tissue penetration and cell membrane resistance.
• the development of systems that can transport these substances into cells has been a hot topic in the treatment of molecular methods.
• intracellular transport is possible by various reagents, electroporation, or heat shock, etc., but in the case of proteins, they move into cells while maintaining their activity. It was a very difficult problem to get a clue to the solution.
• HIV-TAT protein consisting of 11 amino acid sequences, was found to play an important role in the cellular process of transfection of HIV cells. The study was conducted.
• Telomere is a genetic material repeatedly present at the end of a chromosome and is known to prevent damage to the chromosome or binding to another chromosome. Each time a cell divides, the telomeres get slightly shorter. After a certain number of cell divisions, the telomeres become very short, and the cells stop dividing and die. On the other hand, elongation of telomeres is known to prolong cell life. For example, cancer cells secrete telomerase, which prevents telomeres from shortening, so cancer cells can continue to proliferate without dying. .
• One aspect of the invention is to provide a novel peptide. .
• One aspect of the invention is to provide a polynucleotide encoding a novel peptide.
• One aspect of the invention is to provide a cell permeable peptide.
• One aspect of the present invention is to provide a peptide useful as an intracellular active ingredient carrier.
• Another aspect of the present invention is to provide useful peptides for intracellular effective ingredient delivery, in particular for topical delivery to mitochondria.
• Another aspect of the present invention is to provide a useful peptide for topically delivering to the mitochondria an active ingredient for ameliorating, preventing or treating mitochondrial-related diseases or disorders.
• One aspect of the present invention is to provide a conjugate conjugated with the cell-penetrating peptide and the active ingredient (conjugated).
• One aspect of the present invention is to provide a composition comprising a conjugate of a cell-penetrating peptide and an active ingredient.
• One aspect of the present invention is to provide a pharmaceutical composition comprising a conjugate of a cell-penetrating peptide and an active ingredient.
• One aspect of the present invention is to provide a functional cosmetic composition comprising a conjugate of the cell-penetrating peptide and the active ingredient.
• One aspect of the present invention is to provide a health food composition comprising a conjugate of a cell-penetrating peptide and an active ingredient.
• One aspect of the present invention is to provide a contrast agent comprising a conjugate of a cell-penetrating peptide and an active ingredient.
• the conjugate according to one aspect of the present invention is a conjugate of a cell-permeable carrier peptide and an active ingredient, wherein the carrier peptide is any one of SEQ ID NOs: 1 to 6 A peptide comprising, a peptide having at least 80% sequence homology with the depth sequence, or a fragment thereof, wherein the peptide and fragment having at least 80% sequence homology refer to one of SEQ ID NOs: 1 to 6 A conjugate that retains the cellular permeability of the peptide.
• the fragment may be a fragment consisting of three or more amino acids.
• the carrier peptide may be a peptide composed of up to 30 amino acids.
• the carrier peptide is SEQ ID NO:
• the contrast agent according to one aspect of the present invention may be a contrast agent including any one of the conjugates mentioned above.
• the contrast material may be for contrasting the cells.
• the cell may be a stem cell.
• composition according to one aspect of the invention may comprise any of the conjugates mentioned above.
• the active ingredient is an active ingredient for the treatment or prevention of a disease
• the composition may be a pharmaceutical composition.
• the active ingredient is an active ingredient of a functional cosmetic
• the composition may be a cosmetic composition.
• the active ingredient is an active ingredient of a functional health food, and the composition may be a health food composition.
• a method according to an aspect of the present invention is a method for delivering an active ingredient into a cell, comprising administering any one of the above-mentioned conjugates to a subject in need thereof.
• the carrier peptide is a cell-penetrating peptide that performs intracellular delivery of the active ingredient, and peptides and fragments having 80% or more of sequence homology are those of one of SEQ ID NOs: 1 to 6 It may have cell permeability.
• a method for delivering an active ingredient into a cell may be to locally deliver the active ingredient to the intracellular mitochondria.
• the polynucleotide according to one aspect of the present invention may be a polynucleotide encoding the above-mentioned cell permeable peptide.
• the vector according to an aspect of the present invention may be a vector including the polynucleotide.
• the transformed cell according to an aspect of the present invention may be a transformed cell comprising the vector.
• peptides disclosed herein or conjugates in which the peptide and the active ingredient are bound
• the acid it is possible to easily transfer into the cell active ingredients that are difficult to penetrate into the cell. Through this, the efficacy of the active ingredient can be increased, and as a result, the dosage can be lowered, thereby minimizing side effects due to drug administration and increasing the treatment efficiency.
• local delivery to mitochondria can enhance the effectiveness of the improvement, prevention or treatment of mitochondrial-related diseases or disorders.
• even a very small amount of active ingredients can produce excellent effects.
• Conjugation with a contrast agent can be used as a contrast agent to monitor cell transplantation processes or transplanted cells in cell therapy. In particular, it can be effectively used as a contrast agent for stem cells injected in vivo.
• FIG. 1 is a diagram illustrating a method for preparing a recombinant DNA clone in which peptides of SEQ ID NO: 1 to SEQ ID NO: 6 are bound to enhanced reporter genes (enhanced green fluorescent protein, EGFP) It is.
• enhanced reporter genes enhanced green fluorescent protein, EGFP
• 2 to 7 are diagrams showing the number of cells taken up by binding the FITC to the peptide of SEQ ID NO: 1 to SEQ ID NO: 6 and then treated in HeLa cell line and analyzed by flow cytometry.
• the control group was treated only with FITC.
• the cells were treated with Huh7 cell lines and analyzed by flow cytometry.
• the control group was treated only with FITC.
• each human T lymphocyte cell line was treated and analyzed by flow cytometry.
• the control group was treated with FITC only.
• 16 to 21 show the results of measuring cell viability and toxicity after binding FITC to peptides of SEQ ID NO: 1 to SEQ ID NO: 6, treating each of the HeLa cell lines, and culturing.
• Proteins, nucleic acids, peptides or viruses have great potential as therapeutic substances. However, due to its molecular size, its use was limited because it could not penetrate tissues and cell membranes. In addition, even small-sized substances do not penetrate the lipid bilayer of the cell membrane due to their properties and structure. Electricity
• the transcriptional activator protein is known to act as a cell penetrating peptide capable of transporting large active substances into cells. Studies have been actively conducted. In particular, unlike TAT proteins, which are known to cause intracellular toxicity, studies on substances capable of more effectively transferring large molecules such as proteins, nucleic acids, peptides or viruses into target cells without causing toxicity in vivo Proceeded. Thus, through continuous studies, the inventors have found that peptides derived from telomerase have excellent effects as cell-permeable peptides with little cytotoxicity.
• telomerase 8 is the sequence of human telomerase full-length protein.
• the "name” in Table 1 below is to distinguish peptides.
• at least one of the peptides set forth in SEQ ID NO: 1 to SEQ ID NO: 6 includes a "synthetic peptide" synthesized by selecting a peptide at a corresponding position among peptides contained in telomerase.
• the term “pep” refers to a peptide having any sequence of SEQ ID NO: 1 to SEQ ID NO: 6, or a peptide having 80% or more sequence homology with the sequence, or a fragment of the sequence.
• One aspect of the present invention provides a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a peptide having a sequence homology of 80% or more with the peptide sequence, or a polynucleotide encoding a fragment thereof. do .
• the polynucleotides can be used to mass produce peptides.
• polynucleotides encoding peptides By incorporating the vector into the host cell, the peptide can be mass-produced.
• the peptides disclosed herein may include peptides having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% homology. have.
• the peptides disclosed herein, peptides or fragments thereof comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 and one or more amino acids, two or more amino acids, three or more amino acids, four or more amino acids, 5 or more amino acids, 6 or more amino acids, or 7 or more amino acids may comprise a peptide.
• the amino acid change belongs to the property to change the physicochemical properties of the teat.
• amino acid changes can be performed, such as improving the thermal stability of the peptide, altering substrate specificity, changing the optimal pH, and the like.
• amino acid includes D-isomers and modified amino acids as well as 22 standard amino acids that are naturally incorporated into peptides. Accordingly, in one aspect of the present invention, the peptide may be a peptide including D-amino acid. Meanwhile, in another aspect of the present invention, the peptide may include a non-standard amino acid or the like which has been post-translational modified. Examples of post-translational modifications include phosphorylation, glycosylat ion, acylation (eg, acetylation, myristoylat ion and
• Palmitoylation alkylat ions
• chemical properties e.g., beta-elimination deimidization, Deamidation
• structural changes e.g, formation of disulfide bridges
• Changes in amino acids such as changes in amino acids, such as amino groups, carboxyl groups or side chains, caused by chemical reactions occurring in the process of bonding with crossl inkers.
• Peptides disclosed herein can be wild-type peptides identified and isolated from native sources.
• the peptides disclosed herein may be artificial variants in which one or more amino acids comprise an amino acid sequence in which one or more amino acids are substituted and / or inserted compared to the peptides of any one of SEQ ID NOs: 1-6.
• Amino acid changes in the wild type polypeptide as well as in artificial variants include conservative amino acid substitutions that do not significantly affect the folding and / or activity of the protein. Examples of conservative substitutions include basic amino acids (arginine, lysine and histidine), Acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and
• hydrophobic amino acids leucine, isoleucine, valine and methionine
• aromatic amino acids phenylalanine, tryptophan and tyrosine
• small amino acids glycine, alanine, serine and threonine
• the most common exchanges are Ala / Ser, Val / Ile, Asp / Glu, Thr / Ser, Ala / Gly, Ala / Thr, Ser / Asn, Ala / Val, Ser / Gly, Tyr / Phe, Ala / Pro, Lys / Arg, Asp / Asn, Leu / Ile, Leu / Val, Ala / Glu, and Asp / Gly, and vice versa.
• Other examples of conservative substitutions are shown in the following table.
• hydrophobic norleucine, met ⁇ ala, val, leu, ile
• Non-conservative substitutions will be made by exchanging a member of one of these classes for another class. Any cysteine residue that is not involved in maintaining the proper conformation of the peptide can generally be substituted with serine to improve the oxidative stability of the molecule and to prevent abnormal crosslinking. Conversely, cysteine bond (s) can be added to the peptide to improve its stability.
• Another type of amino acid variant of the peptide is a change in the glycosylation pattern of the antibody.
• change is meant the deletion of one or more carbohydrate residues found in the peptide and / or the addition of one or more glycosylation sites that are not present in the peptide.
• Glycosylation of peptides is typically either N-linked or 0-linked, wherein an N-linked carbohydrate residue is attached to the side chain of an asparagine residue.
• Tripeptide sequences asparagine—X-serine and asparagine-X-threonine, where X is any amino acid except proline, are recognition sequences for enzymatic attachment of carbohydrate moieties to the asparagine side chains.
• X is any amino acid except proline
• 0-linked glycosylation means attaching one of the sugars N-acetyl galactosamine, galactose or xylose to hydroxyamino acid, most commonly serine or threonine, but not 5-hydroxyproline or 5-hydroxy Roxylysine can also be used.
• glycosylation sites to the peptide is conveniently performed by changing the amino acid sequence to contain one or more of the above mentioned tripeptide sequences (for N-linked glycosylation sites). Such changes may be made by adding one or more serine or threonine residues to the sequence of the original antibody or by substituting these residues (for a 0-linked glycosylation site).
• One aspect of the present invention provides a cell permeable peptide comprising a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6, or fragments thereof, or a peptide or Peptides having at least 80% sequence homology are provided.
• One aspect of the present invention provides a drug carrier for delivering one or more active ingredients, a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof, or a sequence homology of 80% or more with the peptide sequence. It provides a pharmaceutical composition comprising a peptide having.
• a peptide comprising any one of SEQ ID NOs: 1 to 6, or a fragment thereof, or a peptide having a sequence homology of 803 ⁇ 4> or more with the peptide sequence may act as a safe and excellent cell permeable peptide. In combination with the drug can effectively deliver into the cell.
• a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6, or a fragment thereof, or a peptide having 80% or more sequence homology with the peptide sequence and an active ingredient to be moved are conjugated to each other.
• the active ingredient may be one or more selected from protein, nucleic acid, peptide lipids, glycolipids, minerals, sugars, contrast materials, drugs (compounds) and chemicals (compounds).
• the active ingredient may be a peptide.
• the peptide as an active ingredient may be a cytokine, an antibody, an antibody fragment, a therapeutic enzyme, a soluble receptor, or a ligand.
• CPP Cell Penetrating Peptide
• the term “cargo” includes any substance that can move into a cell in combination with a cell permeable template, for example, any substance that wants to increase cell permeation efficiency, specifically, Active substances in drugs, cosmetics or healthy foods, more specifically substances that are not easily transported into the cell through the general route, and more specifically sugars, nanoparticles such as proteins, nucleic acids, peptides, minerals and glucose Or, but not limited to, biologicals, viruses, contrasts or other chemicals. It is a broad concept including a relief, preventing, treating or substance for diagnosis.
• carrier peptide refers to a peptide that serves to move an active ingredient to a desired site by binding to an active ingredient.
• the protein or peptide to be moved includes one or more of hormones, hormone analogs, enzymes, enzyme inhibitors, signal transduction proteins (or peptides), antibodies and vaccines, including but not limited to no.
• the nucleus Acids may be naturally occurring or artificial DNA or RNA molecules and may be single stranded or double stranded.
• the nucleic acid molecule may be more than one, and may be nucleic acid molecules of the same type (eg, having the same nucleotide sequence), or may be other types of nucleic acid molecules.
• the virus may comprise a viral core comprising the entire virus or a nucleic acid of the virus.
• chemicals are broad concepts, including chemicals that can function as drugs, and include natural or synthetic chemicals.
• the drug delivered into the cell by the cell-penetrating peptide may further comprise a drug carrier such as liposomes, micelles, nanoparticles, magnetic particles, or tubular dots.
• contrast 1 is a broad concept that includes all materials used for the imaging of structures or fluids in vivo in medical imaging.
• Suitable contrast agents are radiopaque contrast media ( radiopaque contrast agents, paramagnetic contrast agents, superparamagnetic contrast agents, computed tomography (CT) contrast agents and other contrast agents, including but not limited to Radiopaque contrast agents (for X-ray imaging) include inorganic iodine compounds and organic iodine compounds (e.g., diatrizoat), radiopaque metals and their salts (e.g.
• radiopaque compounds eg, calcium salts, barium salts such as barium sulfate, tantalum and tantalum oxide.
• Gd-DTPA gadolinium diethylene triamine penta acetic acid
• Gd-DTPA gadolinium di ethylene tr iaminepentaacet ic acid
• Euopium, erbium, crumb, nickel and cobalt complexes for example 1,4,7, 10-tetraazacyclododecane-N, N ', N ", N"' Tetraacetic acid (D0TA), Ethylenediamintetraacetic acid (EDTA), 1,4,7,10-tetraazacyclododecane-N, -N ', ⁇ "-triacetic acid (D03A), 1,4,7 -Triazacyclononane — ⁇ , ⁇ "-triacetic acid (N0TA), 1,4,8,10-tetraazacyclotetradecane ', ⁇ ", ⁇ ' ⁇ tetrateacetic acid ( ⁇ ), hydroxydoxybenzyl Ethylene ⁇ diamine diacetic acid (HBED), etc.
• Suitable superparamagnetics for example 1,4,7, 10-tetraazacyclododecane-N, N ', N ", N"' Tetraacetic acid (
• Contrast material for MR imaging is magnetite, superparamagnetic iron oxide iron oxide (SPIO), ultraparamagnetic iron oxide (USPIO) and monocrystalline iron oxide (monocrystai line).
• SPIO superparamagnetic iron oxide iron oxide
• USPIO ultraparamagnetic iron oxide
• monocrystalline iron oxide monocrystai line
• Other suitable contrast agents may be contrast agents such as iodide and non-iodinated, ionic and nonionic CT contrast agents, and spin-labels, or other diagnostics.
• contrast agents include, but are not limited to, ⁇ -galactosidase, green fluorescent protein, blue fluorescent protein, luciferase, and the like, which encode proteins that are readily detectable when expressed in cells. Marker genes.
• Various labels can be used, such as radionuclides, fluorescents, enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, ligands (especially hapten), and the like.
• the contrast material is of the formula (2)
• Ferrocenecarboxylic acid The structure of ferrocene is shown in Formula 1.
• the conjugate of the cell-penetrating peptide and the contrast material may be Ferrocenecarboxylic -pep (Ferrocenecarboxylic-pep) of the formula (3). ⁇ 75> [Formula 3]
• the peptide or composition may be one or more detectable
• the label can be a compound that can be detected chemically, physically, or enzymatically or directly or indirectly to generate a signal. Labeling and subsequent detection can be performed by methods known in the art (eg, Sambrook, J., and Russel, DW (2001); and Lottspeich, F., and Zorbas H. (1998) Bioanalyt) ik, Spektrum Akademischer Ver 1 ag, Heidelberg / Berlin,
• Labels include, but are not limited to, fluorescent labels, enzyme labels, chromogenic labels, luminescent labels, radiolabeled hapten, biotin, metal complexes, metals and colloidal gold. All these types of labels are well known in the art and are commercially available from various suppliers.
• binding methods such as covalent or non-covalent bonds.
• the subject to be moved may, for example, bind to the N-terminus or C-terminus of the peptide according to one aspect of the invention.
• a peptide may be used via a disulfide bond or a covalent bond that binds the moving object to an alpha amine ( ⁇ -amine) or C-terminal lysine (K) residue of the peptide N-terminal glutamate (E). You can combine the moving target.
• one of the peptides and the moving object may be bound to the peptide and the moving object through a non-covalent bond that surrounds the other in a form such as a capsular form.
• a linker may be used to associate a peptide with a moving object.
• a hyal (6—hydrazinopyridine-3-carboxylic acid, 6-hydrazinopyridine-3-carboxylic acid) linker may be used for the alpha amine of the peptide N-terminal glutamate or the amine of the C-terminal lysine residue.
• the moving object can be bound to the peptide by binding the moving object to the linker.
• the peptide when the moving target is DNA or RA, the peptide is introduced with a SH group (thiol group) and the DNA or RNA with a maleimide group and then a peptide.
• SH group thiol group
• the transfer target By linking the SH group and DNA or RA maleimide group, the transfer target can be bound to the peptide.
• the moving target is a protein or a peptide
• the transport peptide can be coupled to the carrier peptide in the form of a fusion protein.
• Specific examples of the binding by the fusion protein are as follows: In preparing a primer for producing the fusion protein, the nucleotide encoding the carrier peptide is attached to the nucleotide expressing the moving target, followed by the obtained nucleotide. Is inserted into a vector, for example, a pET vector as a restriction enzyme, and expressed by introducing (transformation) BL-2KDE3) into, for example, a cell. At this time,
• Expression inducers such as isopropyl-l-thio- -D-galactopyranoside (IPTG), can be treated to effectively express fusion proteins. After, His tag
• fusion protein expressed through a method such as purification can be purified, and then dialyzed using PBS, and put into a kit, for example, concentrated by centrifugation for 5 to 20 minutes at 2,000 to 4,000 rpm.
• the carrier peptide is capable of binding to a dyeing or fluorescent material, specifically fluorescein isothiocyanate (FITC) or green fluorescent protein (GFP).
• FITC fluorescein isothiocyanate
• GFP green fluorescent protein
• the FTTC may bind to the amino group (N3 ⁇ 4 + ) of the N′-terminal or C-terminal Lys of the carrier peptide.
• the peptide and the FITC may be bound by a linker including Lys.
• a peptide comprising any one of SEQ ID NOs: 1 to 6 as disclosed herein, or a fragment thereof, or a peptide having at least 80% sequence homology with the peptide sequence may be carried.
• the molar ratio of CPP: to be moved may be 2: 1 or more. Specifically, it may be 2: 1 or more, 3: 1 or more, 4: 1 or more, 5: 1 or more, 6: 1 or more, 7: 1 or more, 8: 1 or more, 9: 1 or more, or 10: 1 or more. This means that a plurality of carrier peptides can bind to one molecule of movement.
• the plurality of carrier peptide molecules may be linked in series or in parallel with each other. Linked in series means binding to each other at the terminal amino acid sites of the carrier peptide. In parallel, linked in parallel means binding to each other at portions other than the terminal amino acids of the carrier peptide.
• the molar ratio of carrier peptide: move target can be 1: 2 or more. This means that a plurality of move target molecules can bind to one carrier peptide molecule.
• the molar ratio of the carrier peptide to the moving object may be 1: 2. Specifically, it may be 1: 2 or more, 1: 3 or more, 1: 4 or more, 1: 5 or more, 1: 6 or more, 1: 7 or more, 1: 8 or more, 1: 9 or more, or 1:10 or more. .
• the carrier peptide according to one aspect of the present invention can be used for cell imaging or tracking the intracellular drug delivery route.
• One aspect of the present invention provides a drug for delivering one or more active ingredients of a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof or a peptide having 80% or more sequence homology with the peptide sequence. It serves as a carrier.
• One aspect of the invention is a drug; And applying to a subject a cell comprising a composition comprising a peptide comprising any one of SEQ ID NOs: 1 to 6, or a fragment thereof, or a peptide having at least 80% sequence homology with the peptide sequence.
• a method of delivering a drug is provided.
• a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof, or a peptide having a sequence homology of 80% or more with the peptide sequence, and a contrast agent to the subject.
• a method for tracking the route of application drug delivery to a subject comprising application is provided.
• a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof or a conjugate of a peptide and a contrast material having a sequence homology of 80% or more with the peptide sequence
• a method for tracking the route of application drug delivery to a subject including application to the subject.
• One aspect of the present invention provides a conjugate comprising a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof or a peptide having a sequence homology of 80% or more with the peptide sequence and a conjugate of a drug to be moved.
• a composition comprising; And a kit for intracellular drug delivery to a subject, including instructions indicative of one or more of the dosage, route of administration, frequency of administration, and redness.
• One aspect of the present invention is an active ingredient; And a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or fragments thereof, or 80% of the peptide sequence.
• a cosmetic or food composition comprising a peptide having sequence homology to a phase is provided.
• Another aspect of the present invention comprises a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof, or a conjugate of an active ingredient with a peptide having at least 80% sequence homology with the peptide sequence.
• One aspect of the present invention provides a conjugate of an active ingredient with a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a fragment thereof, or a peptide having 80% or more sequence homology with the peptide sequence. Including, it provides a pharmaceutical, cosmetic or food composition excellent effect of delivering the active ingredient into the cell.
• Mitochondria are the central organs of energy metabolism in nucleated cells and are the first intracellular organs to be associated with human disease (Luft R, Ikkos D, Palmieri G, Ernster L, Afzel ius B: A case of severe hypermetabol i).
• sm of nonthyroid origin with a defect in the maintenance of mitochondrial respiratory control a correlated clinical, biochemical, and morphological study, J CI in Invest 41: 1776 804, 1962).
• the organ is also involved in the regulation of intracellular calcium concentrations, and the mitoconcrete respiratory chain acts as an important electron transport system for energy production and can cause the production of free radicals.
• mitochondrial dysfunction is closely related to adult diseases such as diabetes, cardiomyopathy, infertility, blindness, kidney / liver disease and stroke (Modica-Napolitano KS, Singh KK: April mitochondria as targets for detect ion and treatment of cancer Expert Rev Mol Med 11: 1-19, 2002).
• the mutation of the mitochondrial gene has been suggested to be involved in the invention of aging, neurodegenerative diseases, cancer diseases.
• a mitochondrial targeting active ingredient delivery system may be provided.
• the transport peptide is a peptide that performs local mitochondrial local delivery of the active ingredient as a peptide that migrates locally to the mitochondria in the cell, the sequence homology of> 803 ⁇ 4> Peptides and fragments having a mitochondrial targeting active ingredient delivery system, which possesses the mitochondrial targeting ability of one of SEQ ID NO: 1 to SEQ ID NO: 6 corresponding thereto.
• compositions for controlling mitochondrial activity And a conjugate of any one of the above-mentioned conjugates, wherein the carrier peptide is a peptide that locally migrates into the mitochondria in the cell and is a peptide that performs mitochondrial local delivery of the active ingredient.
• Peptides and fragments having more than 80% sequence homology may be a composition for controlling mitochondrial activity, having the mitochondrial targeting ability of one of the corresponding SEQ ID NO: 1 to SEQ ID NO: 6.
• the composition is a pharmaceutical composition for the treatment, prevention, inhibition of progression, or alleviation of symptoms of mitochondrial-related diseases or disorders. It may be a component that shows the function of treating, preventing, inhibiting progression or alleviating symptoms of a disease or disorder.
• Mitochondrial related diseases may include, but are not limited to: Huntington's disease; sclerosis on the muscular atrophy side; MELAS
• MERRF Myoclonus, epilepsy, and myopathy with ragged red fibers, epilepsy and myopathy of myoclonus with segmented red fibers
• NARP / MILS Neuroogenic muscular weakness, ataxia, retinitis pigmentosa / Maternal ly inherited Leigh syndrome, neuromuscular weakness, ataxia and pigmented retinitis / maternal hereditary Reye syndrome
• LHON Lebers hereditary optic neuropathy, lever's hereditary optic neuropathy, mitochondrial blindness
• KSS Kearns-Sayre Syndrome
• PMPS Pearson Marrow-Pancreas Syndrome, Pearson Bone Marrow-Pancreas Syndrome
• the present invention provides a nucleic acid molecule encoding the polypeptide.
• Nucleic acid molecules can be introduced into host cells according to techniques known to those of skill in the art. For example, GM GCG CGC CCG GCG CTG CTG ATG AGC CGC CTG CGC ⁇ ATT CCG AAA sequence (SEQ ID NO: 181). Nucleic acid molecules can be introduced into host cells according to techniques known to those of skill in the art. For example,
• the host cell may be a higher eukaryotic cell, such as a mammalian cell, or a lower eukaryotic cell, such as a yeast cell, or a prokaryotic cell, such as a bacterial cell.
• Prokaryotic strains suitable for transformation include species belonging to the genus Escherichia coli, Bacillus subtilis, Salmonella typhimurium, Pseudomonas, Strap Thomas and Micbacteria.
• Vectors comprising such nucleic acid molecules generally have a replication origin and selectable markers (e.g., dihydrofolate reductase or neomycin resistance for eukaryotic cell culture) that allow for the transformation of host cells with recombinant expression vectors. Or tetracycline or ampicillin resistance in E. coli, or S. cerevisiae TRP1 gene), and promoters that regulate transcription of protein coating sequences.
• selectable markers e.g., dihydrofolate reductase or neomycin resistance for eukaryotic cell culture
• selectable markers e.g., dihydrofolate reductase or neomycin resistance for eukaryotic cell culture
• promoters that regulate transcription of protein coating sequences.
• Useful expression vectors that can be used are known derivatives such as, for example, SV40 and pcDNA and col El, pCRl, pBR322, pMal-C2, pET, pGEX (Smith, et al., Gene 67: 31-40 (1988))
• Phage DNA such as bacteria pollasmid, pMB9 and its derivative RP4, phage DNA such as numerous derivatives of phage I such as M989, and phage DNA such as M13 and filamentary single-stranded phage DNA;
• Yeast plasmids such as vectors derived from a combination of phage DNA and phage DNA modified for use with phage DNA or expression control sequences.
• Mammalian expression vectors include origins of replication, suitable promoters and enhancers. It may also include essential ribosomal binding sites, polyadenylation sites, splice donor and acceptor sites, transcription termination sequences, and 5 'plumtanking non-transcription sequences. Mammalian expression vectors include inducible promoters, such as those comprising dihydrofolate reductase promoters, Any expression vector containing a DHFR expression cassette or a DHFR / methotrexate co-amplification vector such as pED (Randal J, Kaufman, 1991, Randal J. Kaufman, Current
• GABA glutamine synthetase / methionine sulfoximine co-amplification vector
• pEE14 Celltech
• Vectors that direct episomal expression under the control of Epstein virus (EBV) or nuclear antigen (EBNA), such as pREP4 (Invi trogen), pCEP4 (Invi trogen), pMEP4 (Invi trogen), pREP8 (Invi trogen), pREP9 (Invitrogen), and pEBVHis (Invi trogen) can be used.
• Selective mammalian expression vectors include Rc / CMV (Invi trogen) and pRc / RSV (Invi trogen).
• Vaccinia virus mammalian expression vectors that can be used in the present invention include pSCll, pMJ601, pTKgptFIS and the like.
• Yeast expression systems that can be used in the present invention include non-fusion pYES2.
• the vector can be introduced into a variety of cellular mammals, in particular human-derived cells, as well as bacteria, yeasts, fungi, insects, lamina and plant cells.
• suitable cells include VER0. Cells, HELA cells such as ATCC No. CCL2, CH0 cell lines such as ATCC No.
• COS cells such as COS-7 cells and ATCC No. CRL 1650 cells, W138, BHK, HepG2, 3T3 such as ATCC No. CRL6361, A549, PC12, K562 cells, 293 cells, Sf9 cells, such as ATCC No. CRL1711 and Cvl cells such as ATCC No. CCL70, etc.
• suitable cells include genus of prokaryotic host cell strains, such as E. coli (such as DH5-a strain), Bacillus subtilis, Salmonella typhimurium, or Pseudomonas, Streptomyces and Staphylococcus. There are strains that belong.
• the composition according to one aspect of the present invention is a peptide comprising any one of SEQ ID NO: 1 to SEQ ID NO: 6 or a peptide thereof or 0.1 to 1 mg / mg peptide having a sequence homology of 80% or more of the peptide sequence
• the content may be included in an amount of 1; zg / rag to 0.5 mg / iiig, more specifically 10; zg / mg to 0.1 mg / mg.
• When included in the above range is not only suitable for showing the intended effect of the present invention, but also satisfies both the stability and safety of the composition, it may be appropriate to include in the above range in terms of cost-effectiveness.
• composition according to one aspect of the present invention can be applied to all animals including humans, dogs, chickens, pigs, cows, sheep, guinea pigs or monkeys.
• compositions according to one aspect of the present invention may be administered orally, rectal, transdermal, intravenous, intramuscular, intraperitoneal, intramedullary, intradural or subcutaneous.
• Formulations for oral administration include tablets, pills, soft or hard capsules, granules,
• Formulations for parenteral administration may be, but are not limited to, injections, drops, lotions, ointments, gels, creams, suspensions, emulsions, suppositories, patches or sprays.
• the pharmaceutical composition according to one aspect of the present invention may include additives such as diluents, excipients, lubricants, binders, disintegrants, laxatives, dispersants, surfactants, colorants, flavoring or sweetening agents as necessary.
• additives such as diluents, excipients, lubricants, binders, disintegrants, laxatives, dispersants, surfactants, colorants, flavoring or sweetening agents as necessary.
• Pharmaceutical compositions according to one aspect of the invention may be prepared by conventional methods in the art.
• the active ingredient of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration, or the judgment of the prescriber. Dosage determination based on these factors is within the level of those skilled in the art and
• the daily dose may be for example 0.1 zg / kg / day to 1 g / kg / day, specifically 1 / / kg / day to 10 mg / kg / day, more specifically 10 zg / kg / day to 1 mg / kg / day, more specifically, may be 50 1 ⁇ 2 / kg / day to 100 / kg / day, but is not limited thereto.
• the pharmaceutical composition according to one aspect of the present invention may be administered once to three times a day, but is not limited thereto.
• Cosmetic compositions according to one aspect of the invention may be provided in all formulations suitable for topical application.
• it may be provided in the form of an emulsion obtained by dispersing an oil phase in a solution, an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder paste, a foam, or an aerosol.
• Such formulations may be prepared according to conventional methods in the art.
• the cosmetic composition according to one aspect of the present invention may include other ingredients that may give a synergistic effect to the main effect, preferably within a range that does not impair the main effect.
• the cosmetic composition according to an aspect of the present invention is a moisturizer, an emulsifier, a surfactant, a ultraviolet absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a fragrance, a cooling agent or a restriction agent. It may include.
• the blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount is 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the cosmetic composition Can be.
• the formulation of the food composition according to one aspect of the present invention is not particularly limited, but may be, for example, formulated into tablets, granules, powders, solutions, solid preparations, and the like. Each formulation is formulated or formulated with ingredients commonly used in the art in addition to the active ingredient. Depending on the purpose of use, those skilled in the art can select a suitable formulation without difficulty, and synergistic effects may occur when applied simultaneously with other raw materials.
• the daily dosage thereof is, for example, specifically 1 / g / kg / day to 10 mg / kg / day, more specifically 10 g / kg / day to 1 mg / kg / day, more specifically 50 zg / kg / day to 100 // g / kg / day, but is not limited thereto, and age, health of the subject to be administered It can vary depending on various factors such as condition and complications.
• Preferred embodiments of the invention include the most optimal mode known to the inventors for carrying out the invention. Variations of the preferred embodiments may become apparent to those skilled in the art upon reading the foregoing description. The inventors expect those skilled in the art to make appropriate use of such variations, and the inventors expect the invention to be practiced in a manner different from that described herein. Accordingly, the invention includes all modifications and equivalents of the subject matter referred to in the appended claims, as permitted by patent law. Moreover, any combination of the above-mentioned components within all possible variations It is included in the present invention unless expressly contradicted or clearly contradicted by context. While the invention has been particularly shown and described with reference to exemplary embodiments, those skilled in the art will appreciate that various changes in form and detail may be made without departing from the spirit and scope of the invention as defined by the following claims. .
• Peptides of SEQ ID NO: 1 to SEQ ID NO: 6 were prepared according to the solid phase peptide synthesis known in the art. Specifically, peptides were synthesized by coupling amino acids from the C-terminus one by one through Fmoc solid phase synthesis (SPPS) using ASP48S (Peptron, Inc., Daejeon, Korea). As follows, the first amino acid at the C-terminus of the peptides was attached to the resin. For example:
• a cocktail (Cleavage Cocktail) was added to separate peptides from Resin. 5) Cooling diethyl ether is added to the mixture, followed by centrifugation to precipitate the obtained peptide.
• a conjugate in which peptides of SEQ ID NO: 1 to SEQ ID NO: 6 were conjugated with FITC was prepared as follows.
• a fusion protein of a protein (enhanced green fluorescent protein, EGFP) (SEQ ID NO: 9, at least 10) is prepared by the following method.
• the pEGFP-Nl enhanced green the vector as a template, a fluorescent protein (enhanced green fluorescent protein, EGFP) in pET21a (+) vector (Novagen) for cloning and 7 i to include the hanhyo small TABLE 3
• a fluorescent protein enhanced green fluorescent protein, EGFP
• pET21a (+) vector Novagen
• EGFPF (forward) primer contains Ndel and EcoRI restriction enzyme cleavage site
• EGFP-R reverse included an Xhol restriction enzyme cleavage site and thirty EGFP sequences were added.
• KM including 5 () mM potassium chloride, 0.1% Triton X-100, ⁇ . ⁇ magnesium chloride, and 150 ⁇ of four deoxynucleotide triphosphates (dATP, dTTP, dGTP, dCTP) for gene amplification tris-HCl (tris-HCl, H 9.0) was added a primer and Taq DNA polymerase hyosoeul of 2.5U of lOOpmd to the solution and, 10ng pEGFP plasmid DNA using as a template, polymerase chain half for 5 minutes at 95 ° C in the plasmid pEGFP unggi DNA
• Ndel present in multiple cloning site (MCS) of pET21a vector
• Each F (forward) primer comprises a Ndel restriction enzyme cleavage site and is made to add a pep (CPP) DNA sequence of SEQ ID NO: 1 to SEQ ID NO: 6, each
• R (reverse) primers were constructed to include the EcoRI restriction enzyme cleavage site and to add the pep (CPP) DNA sequence of SEQ ID NO: 1 to SEQ ID NO: 6.
• the synthesized oligonucleotide product was digested with Ndel and EcoRI restriction enzymes.
• pET21a-EGFP-His tag recombinant DNA clone was cloned into Ndel and EcoRI restriction enzyme cleavage sites to prepare pET21a-pep (CPP) -EGFP-His tag recombinant DNA clone of SEQ ID NO: 1 to SEQ ID NO: 6 (Fig. One).
• the protein is isolated by transformation. Specifically, E. coli BL2KDE3) (Invitrogen, Carlsbad, CA, USA) is transformed using a 5ml LB / Empicillin medium and then cultured in 100ml medium. In this case, empicillin is added in a 50 lig / ml ratio. Then, after stirring and incubating at 37 ° C. for about 2 to 3 hours, absorbance was measured and grown to within the range of about 0.6 to 0.8, and then 0.1 mM of IPTG (Isopropyl ⁇ -D-1-thiogalactopyranoside) was used to induce the expression of the fusion protein. After treatment with ⁇ lmM and further incubated at 16 ° C ⁇ 37 ° C for 3 to 16 hours, the cells are centrifuged at 5000 rpm for 5 minutes,
• the expressed protein can be visually confirmed that the cell is overexpressed as a result of centrifugation and the cell becomes greenish. His. Remove the tag purification kit, Ni-TAT Protein Isolation Kit (Prod, # 313.14, Quiagen USA) as directed by the manufacturer.
• the protein is purified by dialysis and concentrated. Specifically dialyzed using sterile 4 ° C PBS. First equilibrate the dialysis bag with PBS, and then take the protein solution separated above using a 5 ml injector and place it in the dialysis bag, and then dialysis overnight at 4 ° C. while stirring. Then, in order to increase the concentration of protein and remove unnecessary substances, dialyzed protein was added to BIBASPIN 20 (Prod, # VS2092, Sartorius Stedin biotech, Germany) and concentrated by centrifugation at 4 ° C at 3,000 rpm.
• BIBASPIN 20 Prod, # VS2092, Sartorius Stedin biotech, Germany
• Human cervical cancer cell line (Homo sapiens cervix) from ATCC HeLa cell line, adenocarcinoma cell line, was treated with 10% fetal calf serum (Invitrogen, USA), ear le's salts, non-essential amino acids, sodium pyruvate and 100 ⁇ ⁇ / ⁇ ⁇ penicillin in MEM (Minimum Essential Medium). 100 units / mi
• Streptomycin was added and cultured in a 37t, 5% C0 2 incubator.
• the cell lines were treated with peptides of SEQ ID NOs: 1 to 6 of the present invention, pep (CPP), and flow cytometry and confocal microscopy analysis to compare the degree of control and uptake. It was.
• the cultured cell line was dispensed into chamber wells and added with 10% fetal calf serum (Invitrogen, USA), 100 iizl penicillin and 100 units / m ⁇ streptomycin to the medium at 37: 5% C0. Incubated for 12 hours in two incubators. After PBS washing, MEM (Minim ⁇
• DAPK4 ' 6-diamidino-2—phenyl indole was stained at room temperature for nuclei, and analyzed by confocal microscopy.
• Huh7 suspended cell line was used as a carcinoma cell lien.
• Huh7 cell lines use RPMI 1640 medium. 10% fetal calf serum (Invitrogen, USA), 100 ug / ml penicillin and 100 units / ml streptomycin were added to the culture medium at 37 ° C and 5% C0 2 .
• Jurkat's floating cell line was used.
• Jurkat cell lines use RPMI 1640 medium. 10% fetal calf serum (Invitrogen, USA), 100 ug / ml penicillin, 100 units / ml streptomycin was added to the culture medium at 37 ° C, 5% CO 2 incubator.
• PBMC peripheral blood mononuclear cells
• the cultured HeLa cell line was dispensed into a 96-well plate and cultured with 10% fetal bovine serum (Invitrogen, USA), 100 g / u penicillin and 100 units. Streptomycin was added and incubated for 12 hours in a 37 ° C, 5% C02 incubator. After PBS washing, MEM (Minimum Essential Medium) was subjected to starvation for 1 hour. Each carrier peptide was incubated at 20 ° C. for 24 hours, and then cell viability and toxicity were analyzed using ⁇ assay. The results are as shown in FIGS. 16 to 21.
• HeLa cell line (adenocarcinoma cell line) was treated with 10% fetal calf serum (Invitrogen, USA), Earle's salts, non-essential amino acids, sodium pyruvate and 100 / g / m ⁇ penicillin and 100 units / mi
• Streptomycin was added and incubated in a 37 ° C, 5% C0 2 incubator.
• a fusion protein is prepared by binding an enhanced green fluorescent protein (EGFP), which is a reporter gene, to peptides of SEQ ID NO: 1 to SEQ ID NO: 6 of the present invention.
• EGFP enhanced green fluorescent protein
• the cultured cell line is dispensed into chamber wells and 10% fetal calf into medium.
• Nuclei were stained at room temperature with DAPI (4 ', 6-diamidino-2-phenylindole) and analyzed by confocal microscopy.

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