WO2014021195A1 - 自動分析装置 - Google Patents
自動分析装置 Download PDFInfo
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- WO2014021195A1 WO2014021195A1 PCT/JP2013/070215 JP2013070215W WO2014021195A1 WO 2014021195 A1 WO2014021195 A1 WO 2014021195A1 JP 2013070215 W JP2013070215 W JP 2013070215W WO 2014021195 A1 WO2014021195 A1 WO 2014021195A1
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- sample
- cleaning
- dispensing nozzle
- nozzle
- sample dispensing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
Definitions
- the present invention relates to an automatic analyzer that performs quantitative or qualitative analysis of components such as blood and urine.
- the measuring method of the automatic analyzer is a method that uses a reagent that reacts with the analyte in the sample and changes the color of the reaction solution (colorimetric analysis), and directly or indirectly specifically binds to the analyte. It is roughly classified into an analysis method (immunoassay) that uses a reagent in which a label is added to a substance to be counted and counts the label.
- an analysis method immunoassay
- a sample is mixed with a predetermined amount of reagent for analysis, but the mixing ratio of the sample and the reagent differs depending on the analysis item, and the amount of sample collected is a relatively large suction volume of about 1 ⁇ L to 20 ⁇ L or more. Need.
- the sample dispensing nozzle is washed while moving from the sample that has been collected to the next sample collection, Carryover is prevented, and degradation of analysis accuracy is suppressed.
- sample dispensing and cleaning are incorporated into one operation cycle, and analysis is performed by repeating this.
- the amount of sample suction is large, the contaminated area in the nozzle increases, so that the nozzle is sufficiently cleaned.
- the nozzle cleaning is performed based on the type of sample or the amount dispensed. An invention that changes time is described.
- Recent automatic analyzers are required to improve processing capacity, and when high-speed processing of 1000 tests or more per unit time is required, the operation per operation cycle must be suppressed to 3.6 s or less.
- the suction amount of the sample is wide, from about 1.0 ⁇ L to about 35 ⁇ L, and it is difficult to take the sample dispensing time and the sufficient nozzle cleaning time within one operation cycle.
- An object of the present invention is to realize an automatic analyzer capable of ensuring sufficient cleaning of a nozzle and suppressing a decrease in analysis accuracy even when the sample processing speed is increased.
- the present invention is configured as follows.
- a sample dispensing mechanism having a sample nozzle that sucks a sample contained in a sample container and discharges it to the reaction container, and a reagent dispensing that sucks the reagent contained in the reagent container and discharges it to the reaction container
- a mechanism for cleaning the sample dispensing nozzle with cleaning water an analysis unit for analyzing the sample contained in the reaction vessel, the sample dispensing mechanism, the reagent dispensing mechanism, the washing mechanism, and
- N is an integer of 2 or more
- an automatic analyzer capable of ensuring sufficient cleaning of a nozzle and suppressing a decrease in analysis accuracy even when the processing speed of a specimen is increased.
- 1 is a schematic configuration diagram of an automatic analyzer to which the present invention is applied. It is a schematic block diagram of the sample dispensing mechanism in one Example of this invention. It is a figure which shows the example of a setting of the washing
- Example 1 of this invention it is a figure which shows the example which performed sample aspiration and discharge from 1 sample.
- Example 1 of this invention it is a figure which shows the other example which performed sample suction and discharge from 1 sample.
- Example 1 of this invention it is a figure which shows the further another example which performed sample aspiration and discharge from 1 sample.
- Example 1 of this invention it is a figure which shows the further another example which performed sample aspiration and discharge from 1 sample.
- Example 1 of this invention it is a figure which shows the further another example which performed sample aspiration and discharge from 1 sample. It is a figure explaining the modification of Example 1 of this invention. It is an operation
- Example 1 It is an operation
- 1 is a schematic configuration diagram of an automatic analyzer to which the present invention is applied. It is operation
- FIG. 1 is a schematic configuration diagram of an automatic analyzer to which the present invention is applied.
- reaction vessels 2 are arranged in a circle on the reaction disk 1.
- a plurality of reagent bottles 10 can be arranged in a circle in the reagent disk 9.
- a sample transport mechanism 17 for moving a rack 16 on which a sample container 15 is placed is installed near the reaction disk 1.
- Reagent dispensing mechanisms 7 and 8 are provided between the reaction disk 1 and the reagent disk 9 to suck the reagent from the reagent bottle 10 and discharge the reagent into the reaction container 2.
- a sample dispensing mechanism 11 that can be rotated and moved up and down is installed between the reaction disk 1 and the sample transport mechanism 17, and this sample dispensing mechanism 11 is a sample dispensing nozzle (abbreviated as a sample nozzle). 11a.
- a sample pump 19 is connected to the sample nozzle 11a. The sample nozzle 11a moves while drawing an arc around the rotation axis of the sample dispensing mechanism 11, sucks the sample from the sample container 15, discharges the sample to the reaction container 2, and performs sample dispensing.
- a reaction vessel 2 Around the reaction disk 1, a reaction vessel 2, a cleaning mechanism 3, a spectrophotometer 4, stirring mechanisms 5 and 6, a reagent disk 9, and a sample transport mechanism 17 are arranged, and a cleaning pump 20 is connected to the cleaning mechanism 3.
- a cleaning pump 20 is connected to the cleaning mechanism 3.
- Washing tanks 13, 30, 31, 32, and 33 are installed on the operation ranges of the reagent dispensing mechanisms 7 and 8, the sample dispensing mechanism 11, and the stirring mechanisms 5 and 6, respectively.
- a reagent pump 18 is connected to the reagent dispensing mechanisms 7 and 8.
- the sample container 15 contains a test sample such as blood and is placed on the rack 16 and carried by the sample transport mechanism 17.
- the sample dispensing mechanism 11 sucks the sample from the sample container 15 located at the sample suction position 15w. Further, each mechanism is connected to the controller 21 and the operation is controlled by the controller 21.
- the controller 21 has a function as an analysis unit that analyzes the inspection sample in the reaction container 2.
- FIG. 2 is a schematic configuration diagram of the sample dispensing mechanism 11 in one embodiment of the present invention.
- a sample nozzle 11a that sucks and discharges a sample can be moved up and down and rotated by an arm 55, and the nozzle 11a is connected to a pipettor 51 that generates a differential pressure for allowing the sample to flow in the flow path.
- a pump 53 that supplies system water to a flow path from the pipetter 51 to the nozzle 11a, a flow path that connects the pump 53 and the pipettor 51, and at least one electromagnetic wave that is in the flow path and controls the flow of the system water.
- a valve 53 is provided. Wash water is stored in the tank 54.
- the cleaning tank 13 for cleaning the sample nozzle 11a includes a pump 57 for supplying cleaning water from the tank 54, a flow path connecting the pump 57 and the cleaning tank 13, and the cleaning water. And a solenoid valve 58 for controlling the flow.
- FIG. 3 is a setting example of the cleaning timing of the nozzle 11a that can be performed in one operation cycle, that is, one operation cycle (T0 to T1 or T1 to T2) in one embodiment of the present invention.
- the time for one cycle is the same as the rotation period of the reaction disk.
- the cleaning times t 1 to t n at the cleaning timing may be the same or different from each other.
- the timing of cleaning the nozzle 11a and the timing of cleaning the reagent nozzles of the reagent dispensing mechanisms 7 and 8 overlap. It is desirable that the cleaning water amount of the reagent nozzle be appropriately controlled and stabilized by fixing the nozzle cleaning execution timing and the cleaning times t 1 to t n even if they are not overlapped or overlapped.
- the cleaning time is not simply extended as described above. If the cleaning time is the same, the nozzle 11a can be divided into a plurality of times. In the second washing, the sample was replaced with washing water by the first washing, and the dirt remaining on the inner wall of the nozzle 11a diffused into the washing water replaced by the first washing by the second washing. More dirt can be removed and the cleaning efficiency can be improved.
- FIG. 4 is a diagram showing a case where two cleaning cycles having two nozzle cleaning timings of the first cleaning timing and the last cleaning timing in FIG. 3 are set twice in one embodiment of the present invention. is there.
- FIG. 5 is a diagram showing a method of cleaning the nozzle 11a when the nozzle 11a performs one sample suction from one specimen, which is an embodiment of the present invention.
- the threshold value is 20 ⁇ L and the sample suction amount of the nozzle is less than 20 ⁇ L
- sample suction, sample discharge, and nozzle cleaning are performed in one operation cycle.
- the sample suction amount of the nozzle is 20 ⁇ L or more
- nozzle cleaning cleaning 1 and cleaning 2
- the sample dispensing mechanism 11 accesses the sample container 15 installed in the rack 16 on the sample transport mechanism 17 located at the sample suction position 15w in one operation cycle from time T0 to T1. Then, a relatively small amount, for example, 6 ⁇ L of the sample is sucked by the sample nozzle 11a. Next, the sample dispensing mechanism 11 accesses the reaction container 2 on the reaction disk 1 and discharges, for example, 1 ⁇ L of the sample.
- the nozzle 11a After completion of the discharge of the sample, the nozzle 11a is moved to the cleaning tank 13 by the sample dispensing mechanism 11, the electromagnetic valve 53 and the electromagnetic valve 58 are opened during the “cleaning 2 ” time shown in FIG. 4, and the outer and inner surfaces of the nozzle 11a. Perform cleaning.
- the sample dispensing mechanism 11 accesses a new sample container 15 installed in the rack 16 on the sample transport mechanism 17, and a relatively large amount, for example, 30 ⁇ L of sample is sampled by the sample nozzle 11a.
- the reaction container 2 on the reaction disk 1 is accessed and, for example, 25 ⁇ L of sample is discharged.
- the nozzle 11 a is moved to the cleaning tank 13 by the sample dispensing mechanism 11.
- the threshold is set to 20 ⁇ L, and the cycle used for cleaning and the number of cleanings can be controlled.
- the threshold value may be set to one or more than one.
- FIG. 6 is an example in which two threshold values are set, and is an operation explanatory diagram in the case where, for example, 35 ⁇ L is set as the second threshold value in addition to the first threshold value. Then, a plurality of examples of the cleaning method when a sample of, for example, 40 ⁇ L is sucked by the sample nozzle 11a are shown in FIGS. 6 (a) to 6 (e). In these examples, when the sample suction amount of the nozzle 11a is equal to or larger than the second threshold value, the cleaning is performed three or four times in the next cycle and further in the next cycle of the cycle in which the sample is sucked and discharged. is there.
- FIG. 6A is an example in which cleaning 1 and cleaning 2 are executed in the next cycle T1 to T2 after the cycles T0 to T1, and further cleaning 1 is executed in the next cycles T2 to T3.
- FIG. 6D is an example in which the cleaning 1 is executed in the next cycle T1 to T2 after the cycles T0 to T1, and the cleaning 1 and the cleaning 2 are further executed in the next cycles T2 to T3.
- FIG. 6E is an example in which the cleaning 2 is executed in the next cycle T1 to T2 after the cycles T0 to T1, and the cleaning 1 and the cleaning 2 are further executed in the next cycles T2 to T3.
- the cleaning of an appropriate amount of the nozzle 11a can be performed at an appropriate timing by providing a plurality of cleaning times and a cleaning cycle. Can be implemented.
- sample suction and sample discharge are performed in multiple cycles in which the first and second cycles are cleaned, so that appropriate cleaning operations can be performed based on the history of sample suction. Can be executed.
- FIG. 7 to 10 are explanatory diagrams in the case of performing sample aspiration twice from one specimen. Also, 20 ⁇ L is set as the sample suction amount threshold, and when the sample suction amount is less than 20 ⁇ L, sample dispensing and washing operations are performed during one cycle T0 to T1 in FIG. In T1 to T2 in FIG. 5, sample dispensing and washing and cleaning operations are performed in T2 to T3.
- FIG. 7 shows an example in which, for example, 6 ⁇ L of sample is sucked in one cycle T0 to T1, and after 1 ⁇ L of sample is discharged, 1 ⁇ L of sample is sucked and 1 ⁇ L of sample is discharged from one sample in the next cycle T1 to T2.
- FIG. 7 shows an example in which, for example, 6 ⁇ L of sample is sucked in one cycle T0 to T1, and after 1 ⁇ L of sample is discharged, 1 ⁇ L of sample is sucked and 1 ⁇ L of sample is discharged from one sample in the next cycle T1 to T2.
- the history of the sample suction amount is 6 ⁇ L for the first sample suction, and then 1 ⁇ L is discharged into the reaction container 2 and 1 ⁇ L is sucked for the second sample suction.
- the suction history by the first and second sample suction is a threshold value of 20 ⁇ L or more
- the dispensing and cleaning operations shown in T1 to T3 in FIG. 5 are performed.
- FIG. 8 shows an example in which, after T0 to T1, for example, 20 ⁇ L sample suction and 15 ⁇ L sample discharge are performed, and then 15 ⁇ uL sample suction and 15 ⁇ L sample discharge are performed from one sample after T1 to T2.
- the first sample suction is 20 ⁇ L
- 15 ⁇ L is discharged to the reaction container 2 and 15 ⁇ L is sucked by the second sample suction, and the remaining sample amount in the nozzle 11a.
- Is 15 ⁇ L + 5 ⁇ L 20 ⁇ L, which exceeds the threshold value of 20 ⁇ L in both cases, so that cleaning is performed in accordance with the cleaning operation of T1 to T3 in FIG.
- FIG. 8 is a two-cycle dispensing that is divided into a dispensing cycle and a cleaning cycle in the case of sample suction of 20 ⁇ L or more.
- Cleaning is omitted, and cleaning is performed twice in the third cycle.
- cleaning is performed in the second dispensing cycle, T2 to T3, from time T1 to T2, and from dispensing to cleaning is completed in three cycles. Since the dispensing cycle and the cleaning cycle are separated, the time per cycle can be shortened.
- FIG. 9 shows an example in which, after T0 to T1, for example, 6 ⁇ L sample suction and 1 ⁇ L sample discharge are performed, and then 15 ⁇ L sample suction and 15 ⁇ L sample discharge are performed from one sample after T1 to T2.
- the first sample suction is 6 ⁇ L
- FIG. 10 shows an example in which, after T0 to T1, for example, 20 ⁇ L of sample suction and 15 ⁇ L sample discharge are performed, 1 ⁇ L sample suction and 1 ⁇ L sample discharge are performed from one sample after T1 to T2.
- the first sample suction is 20 ⁇ L
- the sample suction is 6 ⁇ L, the sample suction time is short, and one nozzle cleaning is possible.
- the two cleanings may be divided into T1 to T2 and T2 to T3.
- A of FIG. 11, for 2 washed with T1 ⁇ T2, it is an example of cleaning 2 even T2 ⁇ T3.
- B in FIG. 11, washed 2 with T1 ⁇ T2, an example of performing washed with T2 ⁇ T3.
- threshold determination is performed for the maximum sample aspiration amount and the third sample aspiration amount of the first and second samples, and four or more samples from one sample are performed.
- threshold determination is performed for the maximum sample suction amount from the first to third times and the fourth suction amount, and the number of nozzle cleanings and timing are determined.
- N is an integer of 2 or more and N samples are sucked N times from the same sample, the maximum sample suction is 1 to N ⁇ 1 times.
- FIGS. 7 to 11 Based on the amount and the N-th sample suction amount immediately before nozzle cleaning, the number and timing of nozzle cleaning are determined without increasing the operation cycle unnecessarily, and nozzle cleaning is performed efficiently.
- FIGS. 7 to 11 two types of nozzle cleaning patterns are shown in FIGS. 7 and 8 to 10, but in FIGS. 8 to 10, there are four types of nozzle cleaning patterns by changing the cleaning timing as shown in FIG. A nozzle cleaning pattern may be used.
- the cleaning operation is not included in the dispensing cycle, and is divided into the dispensing cycle and the cleaning cycle. Can be shortened.
- the nozzle When the sample suction amount of the nozzle is small, the nozzle can be sufficiently cleaned with a short cleaning process, and the sample dispensing time is also short, so the washing operation is performed within the sample dispensing cycle, When the amount of sample suction from the nozzle is large, a cleaning cycle separate from the sample dispensing cycle is set in order to ensure sufficient cleaning of the nozzle.
- FIG. 12 is a diagram for explaining a modification of the first embodiment of the present invention.
- the threshold value is 20 ⁇ L
- a relatively small amount, for example, 6 ⁇ L of a sample is sucked by the nozzle 11a, the reaction container 2 on the reaction disk 1 is accessed, and, for example, 1 ⁇ L of the sample is discharged.
- cleaning 2 is performed within the cycle.
- FIG. 12B shows that when the threshold value is 20 ⁇ L, a relatively large amount, for example, 30 ⁇ L of sample is sucked by the nozzle 11a, and the reaction container 2 on the reaction disk 1 is accessed, for example, 25 ⁇ L of sample. Is discharged. In this example, cleaning 1 and cleaning 2 are performed in the next cycle.
- the sample discharge start time t 1 and the sample discharge end time t 2 in the sample dispensing cycle in the example of FIG. 12A are timings set according to the sample suction amount.
- the sample discharge start time t 3 and the sample discharge end time t 4 in the sample dispensing cycle in the example of FIG. 12B are timings set according to the amount of sample suction.
- the sample discharge start timing and the sample discharge end timing are made variable according to the sample suction amount.
- FIG. 13 is an operation function block diagram in the controller 21 for executing the nozzle cleaning operation in the first embodiment of the present invention described above, and FIG. 14 is an operation flowchart.
- the controller 21 includes a sample suction position arrival determination unit 21a, a sample dispensing mechanism operation control unit 21b, a dispensing number counter 21c, a dispensing amount memory 21d, a dispensing amount determination unit 21e, and a cleaning.
- a pattern memory 21f and a dispensing nozzle cleaning mechanism operation control unit 21g are provided.
- the sample dispensing mechanism operation control unit 21b determines whether or not there is a residual analysis item of the sample stored in the same sample container 15 as described above (step S4). If there is a residual analysis item, the sample nozzle 11a The nozzle cleaning is canceled, and the dispensing counter 21c is updated by the following formula (1) (step S5).
- n n + 1 (1)
- the process returns to step S2, and the sample dispensing mechanism 11 accesses the sample container 15 in the next cycle. Then, a sample is collected by the sample nozzle 11a, and the sample is dispensed by accessing the reaction container 2, and the dispensed amount is stored in the array a [i] of the dispensed amount memory 21d (step S3).
- the sample dispensing mechanism operation control unit 21b determines whether or not there is a residual analysis item of the sample stored in the same sample container 15 (step S4).
- the dispensing amount judgment unit 21e determines whether or not the sample dispensing amount a [n] is less than, for example, a certain dispensing amount threshold value b ⁇ L (step S6).
- step S6 if the n-th sample dispensing amount a [n] is less than the dispensing amount threshold b ⁇ L, the process proceeds to step S8, where the dispensing amount determination unit 21e performs the first to n ⁇ 1th sample dispensing. It is determined whether or not all of the dosages a [1] to a [n ⁇ 1] are less than the dispensing quantity threshold b ⁇ L (step S8). This determination is equivalent to determining whether or not the maximum sample suction amount of the sample dispensing amounts a [1] to a [n ⁇ 1] is less than the dispensing amount threshold b ⁇ L.
- step S8 If it is determined in step S8 that all of the sample dispensing amounts a [1] to a [n ⁇ 1] are less than the dispensing amount threshold b ⁇ L, the cleaning pattern W4 stored in the cleaning pattern memory 21f (for example, , A cleaning pattern within the cycle of T0 to T1 in FIG. 5 is selected (step S12), and the cleaning mechanism (solenoid valves 52, 58 is adjusted by the dispensing nozzle cleaning mechanism operation controller 21g so as to conform to the cleaning pattern W4. The operation of the pipetter 51 and the pumps 53 and 57) is controlled.
- step S8 If it is determined in step S8 that any of the sample dispensing amounts a [1] to a [n-1] is equal to or larger than the dispensing amount threshold b ⁇ L, in other words, the sample dispensing amounts a [1] to a If it is determined that the maximum sample suction amount of [n ⁇ 1] is equal to or greater than the dispensing amount threshold value b ⁇ L, the cleaning pattern W3 stored in the cleaning pattern memory 21f (for example, the cleaning within the cycle of T1 to T3 in FIG. 11) Pattern) is selected (step S11), and the operation of the cleaning mechanism is controlled by the dispensing nozzle cleaning mechanism operation control unit 21g so as to conform to the cleaning pattern W3.
- the cleaning pattern W3 stored in the cleaning pattern memory 21f for example, the cleaning within the cycle of T1 to T3 in FIG. 11) Pattern
- step S6 when the n-th sample dispensing amount a [n] is equal to or greater than the dispensing amount threshold b ⁇ L, the process proceeds to step S7, where the dispensing amount determination unit 21e performs the first to n ⁇ 1th sample dispensing. It is determined whether or not all of the dosages a [1] to a [n ⁇ 1] are less than the dispensing quantity threshold b ⁇ L (step S7).
- step S7 If it is determined in step S7 that all of the sample dispensing amounts a [1] to a [n ⁇ 1] are less than the dispensing amount threshold b ⁇ L, the cleaning pattern W2 stored in the cleaning pattern memory 21f (for example, , A cleaning pattern within the cycle of T1 to T3 in FIG. 5 is selected (step S10), and the cleaning mechanism (solenoid valves 52, 58 is adjusted by the dispensing nozzle cleaning mechanism operation controller 21g so as to conform to the cleaning pattern W2. The operation of the pipetter 51 and the pumps 53 and 57) is controlled.
- step S7 If it is determined in step S7 that any one of the sample dispensing amounts a [1] to a [n ⁇ 1] is equal to or larger than the dispensing amount threshold b ⁇ L, the cleaning pattern W1 ( For example, the cleaning pattern in the cycle from T1 to T3 in FIG. 6C is selected (Step S9), and the operation of the cleaning mechanism is adjusted by the dispensing nozzle cleaning mechanism operation control unit 21g so as to conform to the cleaning pattern W1. To control.
- the n-th dispensing amount threshold value is determined to determine the free time in the cycle after the n-th dispensing and the contamination amount of the nozzle 11a.
- the optimum nozzle cleaning is performed from the empty time after dispensing and the amount of contamination of the nozzle 11a. Can be implemented.
- FIG. 14 illustrates that the nozzle cleaning patterns W1 to W4 are selected in steps 6 to 8.
- the present invention is not limited to the fact that all of the nozzle cleaning patterns W1 to W4 are different.
- the nozzle cleaning patterns W2 and W3 may be the same cleaning pattern.
- the nozzle cleaning patterns W1 to W3 may be the same cleaning pattern.
- At least two types of nozzle cleaning patterns among the nozzle cleaning patterns W1 to W4 may be used.
- the nozzle cleaning pattern W4 does not have a sample suction amount equal to or greater than the dispensing amount threshold b ⁇ L, and the nozzle cleaning patterns W1 to W3 include at least a sample suction amount equal to or greater than the dispensing amount threshold b ⁇ L. Since there is a large difference in the amount, it is desirable that the nozzle cleaning patterns W1 to W3 and W4 are two different types of nozzle cleaning patterns.
- 15 and 16 are diagrams for explaining the operation of the second embodiment of the present invention.
- 20 ⁇ L is set as the sample suction amount threshold value of the nozzle 11a, and the cleaning operation of the nozzle 11a is changed from the history of the sample suction amount of the nozzle 11a.
- the number of cleanings and the cleaning timing are adjusted based on the history of the sample suction amount of the nozzle 11a.
- the cleaning pressure and the cleaning speed are adjusted based on the history of the sample suction amount of the nozzle 11a. Adjust the frequency and cleaning timing.
- FIG. 15 is a figure which shows the example in case the nozzle 11a sucks a sample of 6 microliters, for example, and discharges 1 microliter of sample from one specimen.
- the history of the sample suction amount of the nozzle 11a is 6 ⁇ L, which is below the threshold value of 20 ⁇ L.
- the nozzle 11a is cleaned by setting the pressure to a low pressure, for example, 200 kPa.
- FIG. 15B shows an example in which the nozzle 11a sucks a sample of 20 ⁇ L, for example, and discharges a sample of 15 ⁇ L from one sample.
- the suction volume history is 20 ⁇ L, which is equal to or greater than the threshold of 20 ⁇ L. Therefore, the pressure of the sample dispensing and the pump 53 is increased during the period T0 to T1.
- the nozzle 11a is cleaned at 400 kPa.
- the cleaning amount of the nozzle 11a can be obtained about 1.4 times in the same cleaning time. Therefore, optimal nozzle cleaning according to the sample suction amount of the nozzle 11a can be performed without increasing the operation cycle.
- FIG. 16 shows another example of the second embodiment of the present invention.
- FIG. 16 is an example when the first threshold value is 20 ⁇ L and the second threshold value is 35 ⁇ L, for example.
- the cleaning shown in FIG. 16 is less than 20 ⁇ L, the cleaning shown in FIG.
- Example 1 when performing sample aspiration several times from the same sample, the frequency
- FIG. 14 That is, according to the flowchart of FIG. 14, any one of the nozzle cleaning patterns W1 to W4 is selected and executed.
- FIG. 17 is a schematic configuration diagram of an automatic analyzer to which the present invention is applied.
- FIG. 17 includes a sample dispensing mechanism 12 in the automatic analyzer to which the present invention of FIG. 1 is applied. Similar to the sample dispensing mechanism 11, the sample dispensing mechanism 12 includes a sample nozzle 12a, and a sample pump 19 is connected to the sample nozzle 12a. The sample nozzle 12a moves while drawing an arc around the rotation axis of the sample dispensing mechanism 12, sucks the sample from the sample container 15 located at the sample suction position 15W2, discharges the sample to the reaction container 2, and dispenses the sample. I do.
- FIG. 18 is a diagram illustrating the operation of the third embodiment of the invention.
- 20 ⁇ L is set as the sample suction amount threshold value of the nozzles 11a and 12a, and the cleaning operation of the nozzles 11a and 12 is changed from the history of the sample suction amounts of the nozzles 11a and 12a.
- FIG. 18 has a total of three cleaning timings, the first cleaning timing of one cycle in FIG. 3 and the last two cleaning timings.
- FIG. 18A is a diagram showing an example in which the nozzle 11a and the nozzle 12a suck a sample of 6 ⁇ L, for example, and discharge a sample of 1 ⁇ L from one sample.
- the history of the sample suction amount of the nozzles 11a and 12a is 6 ⁇ L, which is below the threshold value of 20 ⁇ L.
- the nozzle 11a is cleaned during the “cleaning 2” time, and the nozzle 12a is cleaned during the “cleaning 3” time.
- FIG. 18B is a diagram illustrating an example in which the nozzle 11a and the nozzle 12a perform, for example, 30 ⁇ L of sample suction and 25 ⁇ L of sample discharge from one sample.
- the history of the sample suction amount of the nozzles 11a and 12a is 30 ⁇ L, which exceeds the threshold value of 20 ⁇ L.
- Dispensing is performed, and cleaning is performed at the times of “washing 1” and “washing 2” in cycles T1 to T2.
- the nozzle 12a dispenses the sample within the cycles T0 to T1 and performs the first cleaning during the “wash 2” time, and performs the second cleaning during the “wash 3” time within the cycles T1 to T2.
- the cleaning cycle is not increased, and cleaning interference between the sample nozzle 11a and the sample nozzle 12a can be avoided.
- the cleaning power is reduced without decreasing the discharge pressure of each cleaning liquid. It is possible to perform cleaning without incurring. This is because the discharge of the cleaning liquid does not occur at the same time and the dispersion of the discharge pressure can be suppressed.
- this avoidance of cleaning interference can be realized by shutting off the cleaning liquid supply valve for the other nozzle immediately before supplying the cleaning liquid to one nozzle. In FIG.
- Example 1 when performing sample aspiration several times from the same sample, the frequency
- FIG. 14 That is, according to the flowchart of FIG. 14, any one of the nozzle cleaning patterns W1 to W4 is selected and executed.
- Example 3 of the present invention the same effect as Example 1 can be obtained.
- sample suction position arrival determination unit 21b ... sample dispensing mechanism operation control unit, 21c ... Dispensing counter 21d: Dispensing amount memory, 21e: Dispensing amount judgment unit, 21f: Cleaning pattern memory, 21g: Dispensing nozzle cleaning mechanism operation control unit, 30: Cleaning tank, 31, 32 33 ... Washing tank, 51 ... Pipetter, 52, 58 ... Solenoid valve, 53, 57 ... Pump, 54 ... Tank, 55 ... Arm
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Abstract
Description
図1は、本発明が適用される自動分析装置の概略構成図である。
分注回数カウンタ21cの更新後、ステップS2に戻り、次サイクルにおいて、試料分注機構11は試料容器15にアクセスする。そして、試料ノズル11aで試料を採取して、反応容器2にアクセスし試料分注を行い、分注量を分注量メモリ21dの配列a[i]へ格納する(ステップS3)。
次に、本発明の実施例2について、図15、図16を参照して説明する。
次に、本発明の実施例3について、図17、図18を参照して説明する。
Claims (5)
- 試料容器に収容された試料を吸引し、反応容器に吐出する試料ノズルを有する試料分注機構と、
試薬容器に収容された試薬を吸引し、反応容器に吐出する試薬分注機構と、
上記試料分注ノズルを洗浄水により洗浄する洗浄機構と、
上記反応容器に収容された試料を分析する分析部と、
上記試料分注機構、上記試薬分注機構、上記洗浄機構、及び上記分析部の動作を一定の動作周期に従って制御し、Nを2以上の整数としたとき、同一試料からN回試料吸引を行う場合、上記試料分注ノズルの1~N-1回までの最大試料吸引量と、N回目の上記試料分注ノズルの試料吸引量とから上記洗浄機構による上記試料分注ノズルの、洗浄回数及び洗浄タイミングを設定し、上記試料分注ノズルの洗浄動作を制御するコントローラと、
を備えることを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置において、
上記コントローラは、上記試料分注ノズルの1~N-1回までの最大試料吸引量と、N回目の上記試料分注ノズルの試料吸引量が、一定の閾値以上か否かを判定し、
上記試料分注ノズルの1~N-1回までの試料吸引量及びN回目の上記試料分注ノズルの試料吸引量が一定の閾値未満の場合は、上記N回目の試料分注ノズルが試料を吸引し、吐出したと同一の動作周期内で、上記洗浄機構による上記試料分注ノズルの洗浄を1回行い、
上記試料分注ノズルのN回目の上記試料分注ノズルの試料吸引量が上記一定の閾値以上の場合は、上記N回目の試料分注ノズルが試料を吸引し、吐出した動作周期の次の動作周期以降で、上記洗浄機構による上記試料分注ノズルの洗浄を複数回行うことを特徴とする自動分析装置。 - 請求項2に記載の自動分析装置において、
上記コントローラは、上記試料分注ノズルのN回目の上記試料分注ノズルの試料吸引量が上記一定の閾値未満の場合であって、上記試料分注ノズルの1~N-1回までの最大試吸引量が上記一定の閾値以上であるときは、上記N回目の試料分注ノズルが試料を吸引し、吐出した動作周期及び次の動作周期で、上記洗浄機構による上記試料分注ノズルの洗浄を複数回行うことを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置において、
上記コントローラは、上記試料分注ノズルの試料吸引量が第1の閾値以上か否かを判定し、上記試料分注ノズルの試料吸引量が上記第1の閾値未満の場合は、上記洗浄機構による上記試料分注ノズルの洗浄圧力を第1の圧力に設定し、上記試料分注ノズルの試料吸引量が上記第1の閾値以上の場合は、上記洗浄機構による上記試料分注ノズルの洗浄圧力を上記第1の圧力より高い第2の圧力に設定し、上記試料分注ノズルが試料を吸引し、吐出したと同一の動作周期内で、上記洗浄機構による上記試料分注ノズルの洗浄を行うことを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置において、
上記コントローラは、上記試料分注ノズルの試料吸引量が第1の閾値以上か否か及び上記第1の閾値より大の第2の閾値以上か否かを判定し、上記試料分注ノズルの試料吸引量が上記第1の閾値未満の場合は、上記洗浄機構による上記試料分注ノズルの洗浄圧力を第1の圧力に設定し、上記試料分注ノズルが試料を吸引し、吐出したと同一の動作周期内で、上記洗浄機構による上記試料分注ノズルの洗浄を行い、
上記試料分注ノズルの試料吸引量が上記第1の閾値以上であって、上記第2の閾値未満の場合は、上記洗浄機構による上記試料分注ノズルの洗浄圧力を上記第1の圧力に設定し、上記試料分注ノズルが試料を吸引し、吐出した動作周期の次の動作周期内で、2回の洗浄を行い、
上記試料分注ノズルの試料吸引量が上記第2の閾値以上の場合は、上記洗浄機構による上記試料分注ノズルの洗浄圧力を上記第1の圧力より高い第2の圧力に設定し、上記試料分注ノズルが試料を吸引し、吐出した動作周期の次の動作周期内で、2回の洗浄を行うことを特徴とする自動分析装置。
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