WO2013168401A1 - センサーチップ並びにセンサーカートリッジおよび検出装置 - Google Patents

センサーチップ並びにセンサーカートリッジおよび検出装置 Download PDF

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Publication number
WO2013168401A1
WO2013168401A1 PCT/JP2013/002922 JP2013002922W WO2013168401A1 WO 2013168401 A1 WO2013168401 A1 WO 2013168401A1 JP 2013002922 W JP2013002922 W JP 2013002922W WO 2013168401 A1 WO2013168401 A1 WO 2013168401A1
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Prior art keywords
metal
light
dielectric layer
nanobody
long
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Ceased
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PCT/JP2013/002922
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English (en)
French (fr)
Japanese (ja)
Inventor
哲雄 眞野
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Seiko Epson Corp
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Seiko Epson Corp
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Application filed by Seiko Epson Corp filed Critical Seiko Epson Corp
Priority to EP13787635.5A priority Critical patent/EP2848920A4/en
Priority to CN201380024597.7A priority patent/CN104303046A/zh
Priority to US14/400,049 priority patent/US20150098085A1/en
Publication of WO2013168401A1 publication Critical patent/WO2013168401A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers

Definitions

  • the present invention relates to a sensor chip including a metal nanobody such as a metal nanoparticle or a metal nanoprotrusion, and a sensor cartridge and a detection device using the sensor chip.
  • a sensor chip using localized surface plasmon resonance (LSPR) is known.
  • LSPR localized surface plasmon resonance
  • Such a sensor chip comprises, for example, metal nanoparticles or metal nanoparticles dispersed on a dielectric surface.
  • the metal nanoparticles are formed sufficiently smaller than the wavelength of the excitation light.
  • all electric dipoles are aligned and an enhanced electric field is induced.
  • near-field light is generated on the surface of the metal nanoparticles.
  • a so-called hot spot is formed.
  • sensor chips using propagation surface plasmon resonance are known.
  • a metal grid is formed on the surface of the element chip.
  • the excitation light is converted into diffracted light by the action of the metal grating.
  • Diffracted light causes propagation surface plasmon resonance at the surface of the metal grating. If such propagation surface plasmon resonance is combined with localized surface plasmon resonance, it is considered that near-field light is enhanced on the surface of the metal nanobody.
  • a sensor chip capable of reliably linking propagation surface plasmon resonance to localized surface plasmon resonance of a metal nanobody can be provided.
  • a metal grid in which a plurality of long metal pieces extending in a first direction are arranged at a pitch shorter than the wavelength of excitation light, covers a surface of the metal grid, and extends in the first direction.
  • the present invention relates to a sensor chip including a dielectric layer that forms a linear concavo-convex pattern extending in the intersecting second direction, and a metal nanobody disposed on the surface of the dielectric layer.
  • LSPR localized surface plasmon resonance
  • the metal nanobody Since the metal nanobody is arranged along a linear uneven pattern, localized surface plasmon resonance (LSPR) is excited in the metal nanobody based on the polarization component parallel to the second direction. At the same time, the polarization component parallel to the second direction is converted into diffracted light by the action of the metal grating. Diffracted light causes propagating surface plasmon resonance (PSPR) at the surface of the metal grating.
  • PSPR surface plasmon resonance
  • Near-field light is enhanced at the surface of the metal nanobody. A so-called hot spot is formed.
  • the intersection angle at which the first direction and the second direction intersect may be set to 90 degrees. According to such a setting, the localized surface plasmon resonance of the metal nanobody can be enhanced to the maximum with one polarization component, and the propagation surface plasmon resonance of the metal lattice can be enhanced to the maximum. Surface plasmon resonance can be efficiently enhanced by linearly polarized excitation light.
  • the metal nanoparticles may be metal nanoparticles dispersed on the surface of the dielectric layer.
  • Metal nanoparticles can be formed with relatively few work steps based on simple metal material deposition or heat treatment. The adoption of such metal nanoparticles can contribute to the simplification of sensor chip manufacturing.
  • the concavo-convex pattern may include long dielectric pieces arranged on a reference plane parallel to the surface of the metal grid and extending in parallel with each other, and the pitch of the arrangement of the long dielectric pieces may be the metal pitch. It is smaller than the pitch of the lattice. According to such an arrangement pitch, the optical anisotropy of the aggregate of metal nano-body can be surely adjusted in a desired direction.
  • a casing that divides a detection chamber, a base having a surface in contact with the space of the detection chamber, and a plurality of metals that are disposed on the surface of the base and extend in the first direction.
  • a metal grid in which long pieces are arranged at a pitch shorter than the wavelength of the excitation light, and a dielectric layer that covers the surface of the metal grid and forms a linear concavo-convex pattern extending in a second direction intersecting the first direction
  • a sensor cartridge comprising a metal nanobody disposed on the surface of the dielectric layer.
  • localized surface plasmon resonance is enhanced by propagation surface plasmon resonance.
  • Near-field light is enhanced at the surface of the metal nanobody. A so-called hot spot is formed.
  • a metal grid in which a plurality of long metal pieces extending in the first direction are arranged at a pitch shorter than the wavelength of the excitation light, and covers the surface of the metal grid, A dielectric layer that forms a linear concavo-convex pattern extending in a second direction intersecting the direction, a metal nanobody disposed on a surface of the dielectric layer, and a light source that emits light toward the metal nanobody,
  • the present invention relates to a detection device including a photodetector that detects light emitted from the metal nanobody in response to the light irradiation.
  • this detection apparatus as described above, localized surface plasmon resonance is enhanced by propagation surface plasmon resonance. Near-field light is enhanced at the surface of the metal nanobody. A so-called hot spot is formed.
  • SERS surface enhancement Raman scattering
  • a silicon dioxide wafer schematically showing a resist film for a lattice pattern.
  • a silicon dioxide wafer schematically showing a lattice pattern.
  • a silicon dioxide wafer schematically showing a metal film on a lattice pattern.
  • a silicon dioxide wafer schematically showing a dielectric layer on a metal film.
  • FIG. 1 schematically shows a sensor chip 11 according to an embodiment of the present invention.
  • the sensor chip 11 includes a base 12.
  • the base 12 is made of a dielectric material, for example.
  • a dielectric material for example, a molding material such as silicon dioxide (SiO 2 ) or a resin material can be used.
  • the resin material may include an acrylic resin such as polymethyl methacrylate resin (PMMA resin).
  • a lattice pattern 13 is formed on the surface of the substrate 12.
  • the lattice pattern 13 has a plurality of long pieces 14 extending in the first direction FD.
  • the long pieces 14 are arranged at an equal pitch in the second direction SD that intersects the first direction FD.
  • the crossing angle at which the first direction FD and the second direction SD intersect in the virtual plane including the surface of the base 12 is set to 90 degrees.
  • a metal film 15 is laminated on the surface of the substrate 12.
  • the lattice pattern 13 of the substrate 12 is covered with a metal film 15.
  • the metal film 15 is made of metal.
  • the metal film 15 can be made of, for example, gold (Au).
  • Other metals include silver (Ag), copper (Cu), aluminum (Al), platinum (Pt), nickel (Ni), palladium (Pd), tungsten (W), rhodium (Rh) or ruthenium (Ru). May be used. These may be used alone, may be used as a laminate of different metal layers, or may be used as an alloy.
  • the metal film 15 can be formed on the surface of the substrate 12 without interruption.
  • the metal film 15 can be formed with a uniform film thickness.
  • the film thickness of the metal film 15 can be set to about 20 nm, for example.
  • the metal film 15 forms a metal lattice 16 following the lattice pattern 13 of the base 12.
  • the metal grid 16 has a plurality of long metal pieces 17 extending in the first direction FD.
  • the long metal pieces 17 are arranged at the first pitch PC in the second direction SD.
  • the first pitch PC is shorter than the wavelength of the excitation light.
  • the long metal pieces 17 can be arranged at equal intervals.
  • the base 12 can be made of a metal material, and the metal film 15 and the base 12 can be formed as one lump.
  • a dielectric layer 18 is laminated on the surface of the metal film 15.
  • the surface of the metal film 15 is covered with a dielectric layer 18.
  • the dielectric layer 18 is formed from a dielectric.
  • the dielectric layer 18 can be formed of, for example, silicon dioxide (SiO 2 ).
  • SiO 2 silicon dioxide
  • the dielectric layer 18 can be formed on the surface of the metal film 15 without interruption.
  • the dielectric layer 18 can be formed with a uniform film thickness.
  • the dielectric layer 18 forms a linear uneven pattern 19 extending in the second direction SD.
  • the concavo-convex pattern 19 has a plurality of long dielectric pieces 21 extending in parallel with each other in the second direction SD.
  • the long dielectric piece 21 is disposed on a reference plane 22 parallel to the surface of the metal grating 16.
  • the long dielectric pieces 21 are arranged at the second pitch CH in the first direction FD.
  • the second pitch CH is smaller than the first pitch PC.
  • the long dielectric pieces 21 can be arranged at equal intervals.
  • the uneven pattern 19 not only the long dielectric pieces 21 are arranged on the long metal pieces 17 but also the long metal pieces 21 are arranged between the long metal pieces 17 (so-called grooves).
  • a metal nanobody 23 is disposed on the surface of the dielectric layer 18.
  • the metal nanobody 23 is composed of metal nanoparticles dispersed on the surface of the dielectric layer 18, for example. Metal nanoparticles are formed with a so-called island structure.
  • the metal nanobody 23 is formed from a metal.
  • the metal nanobody 23 can be formed from, for example, silver (Ag).
  • Other metals include gold (Au), copper (Cu), aluminum (Al), platinum (Pt), nickel (Ni), palladium (Pd), tungsten (W), rhodium (Rh) or ruthenium (Ru). May be used. These may be used alone, may be used as a laminate of different metal layers, or may be used as an alloy.
  • the metal nano-objects 23 are arranged on the long metal piece 17 on the long dielectric piece 21 and also between the long dielectric pieces 21 (grooves), and are dielectric between the long metal pieces 17. It is arranged on the long body piece 21 and also between the long dielectric pieces 21 (grooves).
  • LSPR localized surface plasmon resonance
  • An enhanced electric field is formed between adjacent metal nano bodies 23.
  • Near-field light is generated on the surface of the metal nanobody 23. Since the metal nano bodies 23 are arranged along the linear concavo-convex pattern 19, the enhanced electric field can be enhanced based on the polarization component parallel to the first direction FD. At the same time, the polarization component parallel to the first direction FD is converted into diffracted light by the action of the metal grating 16. The diffracted light causes propagation surface plasmon resonance (PSPR) at the surface of the metal grating 16.
  • PSPR propagation surface plasmon resonance
  • Near-field light is enhanced on the surface of the metal nanobody 23. A so-called hot spot is formed.
  • the long metal pieces 17 of the metal grid 16 extend in the first direction FD.
  • the long dielectric piece 21 of the concavo-convex pattern 19 extends in the second direction SD.
  • the first direction FD and the second direction SD are orthogonal to each other. Accordingly, the localized surface plasmon resonance of the metal nanobody 23 can be enhanced to the maximum with one polarization component, and the propagation surface plasmon resonance of the metal grating 16 can be enhanced to the maximum. Surface plasmon resonance can be efficiently enhanced by linearly polarized excitation light.
  • the metal nanobody 23 is composed of island-structured metal nanoparticles.
  • Metal nanoparticles can be formed with relatively few work steps based on simple metal material deposition or heat treatment. The adoption of such metal nanoparticles can contribute to the simplification of the production of the sensor chip 11.
  • metal nanoprojections can be used instead of metal nanoparticles.
  • the metal nanoprotrusions can be regularly arranged on the surface of the dielectric layer 18. For the formation of such metal nanoprotrusions, for example, a photolithography technique can be used.
  • the second pitch CH of the long dielectric piece 21 is smaller than the first pitch PC of the long metal piece 17.
  • the optical anisotropy of the aggregate of the metal nano bodies 23 can be surely adjusted to the second direction SD.
  • the second pitch CH can take a minimum value.
  • the optical anisotropy of the aggregate of the metal nano bodies 23 is maximized.
  • SERS Surface enhanced Raman scattering
  • FIG. 2 shows the dispersion relation of surface plasmons excited on the metal surface.
  • the dispersion relation of the surface plasmon propagating through the interface between the metal having the dielectric function ⁇ ( ⁇ ) and the medium having the induction function ⁇ m ( ⁇ ) is given by the following equation.
  • ⁇ is the angular frequency
  • k spp is the magnitude of the wave number vector of the surface plasmon propagating along the interface between the metal and the medium
  • c is the speed of light.
  • the dispersion relation between the light incident at the incident angle ⁇ and the light diffracted by the metal grating 16 is given by the following equation.
  • the wave vector magnitude k 0 of the incident light is always larger than k spp and the two dispersion curves do not have intersections, the incident light cannot directly excite surface plasmons. Since the magnitude k n of the wave vector of the n-th order diffracted light has an intersection with the k spp dispersion curve, the n-th order diffracted light can excite surface plasmons.
  • the first pitch PC of the metal grating 16 when having an intersection is always smaller than the light wavelength ⁇ . Therefore, the period of the metal grating 16 is always smaller than the wavelength of the incident light.
  • the present inventor observed the surface enhanced Raman scattering (SERS) spectrum of the metal grating 16.
  • the first pitch PC of the long metal piece 17 was set to 500 nm. Formation of the dielectric layer 18 and the metal nanobody 23 was omitted. Therefore, the metal grid 16 was directly exposed to excitation light. Excitation light having a wavelength of excitation light of 633 nm was used. A He—Ne laser was used as the light source. The sensor chip was irradiated with a linearly polarized beam. Adenine molecules were used as samples. As shown in FIG. 3, a signal peak unique to the adenine molecule, that is, a wave number of 730 cm ⁇ 1 was observed.
  • SERS surface enhanced Raman scattering
  • the present inventor observed the transmittance characteristics of metal nanoparticles having an island structure. Silver nanoparticles were used for the metal nanoparticles. In the observation, a linear uneven pattern 19 was formed.
  • the second pitch CH of the long dielectric piece 21 was set to 140 nm.
  • the direction (second direction) parallel to the linear concavo-convex pattern 19 as compared to the linearly polarized light in the direction orthogonal to the linear concavo-convex pattern 19 (direction parallel to the first direction FD) TM.
  • Low transmittance was observed with linearly polarized TE) (direction parallel to SD).
  • the present inventor observed the SERS spectrum of the silver nanoparticles.
  • silver nanoparticles were formed on the linear uneven pattern 19.
  • the second pitch CH of the long dielectric piece 21 was set to 140 nm.
  • Excitation light having a wavelength of excitation light of 633 nm was used.
  • a He—Ne laser was used as the light source.
  • the sensor chip was irradiated with a linearly polarized beam.
  • Adenine molecules were used as samples. As shown in FIG. 6, a signal peak unique to the adenine molecule, that is, a wave number of 730 cm ⁇ 1 was observed.
  • the present inventor observed the relationship between the height of the long metal piece 17 and the reflectance of light.
  • a metal grid 16 was formed for observation.
  • the first pitch PC of the long metal piece 17 was set to 500 nm. Formation of the dielectric layer 18 and the metal nanobody 23 was omitted. Therefore, the metal grid 16 was directly exposed to excitation light.
  • a drop in reflectivity indicates excitation of surface plasmon resonance.
  • FIG. 7 when the height of the long metal piece 17 was set to 30 to 60 nm, a sufficient drop in reflectance was observed.
  • the height of the long metal piece 17 was set to 40 nm, it was confirmed that light was utilized to the maximum for surface plasmon resonance. It was confirmed that the optimum wavelength for surface plasmon resonance shifts according to the height of the long metal piece 17.
  • a dielectric layer 18, that is, a silicon oxide layer was formed on a flat silver film.
  • Silver nanoparticles having an island-like structure were formed on the surface of the silicon oxide layer.
  • Silver nanoparticles were exposed to excitation light. Excitation light having a wavelength of excitation light of 633 nm was used.
  • a He—Ne laser was used as the light source.
  • the sensor chip was irradiated with a linearly polarized beam. Adenine molecules were used as samples. As shown in FIG. 8, it was confirmed that when the film thickness of the silicon oxide layer was set to about 20 to 40 nm, the electric field strength was maximally increased.
  • a manufacturing method of the sensor chip 11 will be briefly described.
  • a lattice pattern 13 is formed by a resist film 27 on the surface of the silicon dioxide wafer 26.
  • a photoresist is applied to the entire surface of the silicon dioxide wafer 26. Laser interference exposure is performed on the photoresist. After exposure, the photoresist is developed. In this way, a resist film 27 that is shaped like the long piece 14 is formed.
  • the surface of the silicon dioxide wafer 26 is subsequently subjected to an etching process.
  • the resist film 27 functions as a mask.
  • the surface of the silicon dioxide wafer 26 is etched around the resist film 27.
  • the lattice pattern 13 is formed on the surface of the silicon dioxide wafer 26. After the lattice pattern 13 is formed, the resist film 27 is removed.
  • the metal film 15 is formed on the surface of the silicon dioxide wafer 26. For example, gold sputtering is performed. For example, the metal film 15 is formed on the lattice pattern 13 with a uniform film thickness. As a result, the metal film 15 forms a metal lattice 16 following the lattice pattern 13.
  • a dielectric layer 18 is formed on the surface of the metal film 15.
  • sputtering of silicon dioxide can be used to form the dielectric layer 18.
  • the dielectric layer 18 is formed with a uniform film thickness. As a result, the dielectric layer 18 forms a lattice pattern following the metal lattice 16.
  • a striped pattern of the uneven pattern 19 is formed on the surface of the dielectric layer 18 by the resist film 28.
  • a photoresist is applied to the entire surface of the dielectric layer 18. Laser interference exposure is performed on the photoresist. After exposure, the photoresist is developed. In this way, a resist film 28 that is shaped like the long dielectric piece 21 is formed.
  • the dielectric layer 18 is etched.
  • the resist film 28 functions as a mask.
  • the surface of the dielectric layer 18 is etched around the resist film 28.
  • an uneven pattern 19 is formed on the surface of the dielectric layer 18.
  • the resist film 28 is removed.
  • island-shaped silver nanoparticles are formed on the surface of the dielectric layer 18.
  • Silver vacuum evaporation is performed. After the formation of the silver nanoparticles, the individual sensor chips 11 are cut out from the silicon dioxide wafer 26.
  • FIG. 15 schematically shows a target molecule detection device (detection device) 31 according to one embodiment.
  • the target molecule detection device 31 includes a sensor cartridge 32.
  • An introduction passage 33 and a discharge passage 34 are individually connected to the sensor cartridge 32.
  • Gas is introduced into the sensor cartridge 32 from the introduction passage 33.
  • the gas is discharged from the sensor cartridge 32 to the discharge passage 34.
  • a filter 36 is installed at the passage inlet 35 of the introduction passage 33.
  • the filter 36 can remove dust and water vapor in the gas.
  • a suction unit 38 is installed at the passage outlet 37 of the discharge passage 34.
  • the suction unit 38 is constituted by a blower fan.
  • the gas flows through the introduction passage 33, the sensor cartridge 32, and the discharge passage 34 in order.
  • Shutters (not shown) are installed before and after the sensor cartridge 32 in the gas flow path.
  • the gas can be confined in the sensor cartridge 32 according to the opening / closing of the shutter.
  • the sensor cartridge 32 can be detachably attached to the introduction passage 33 and the discharge passage 34, for example.
  • the target molecule detection device 31 includes a Raman scattered light detection unit 41.
  • the Raman scattered light detection unit 41 detects the Raman scattered light by irradiating the sensor cartridge 32 with excitation light.
  • a light source 42 is incorporated in the Raman scattered light detection unit 41.
  • a laser light source can be used as the light source 42.
  • the laser light source can emit linearly polarized laser light at a specific wavelength (single wavelength).
  • the Raman scattered light detection unit 41 includes a light receiving element 43.
  • the light receiving element 43 can detect the intensity of light, for example.
  • the light receiving element 43 can output a detection current according to the intensity of light. Therefore, the intensity of light can be specified according to the magnitude of the current output from the light receiving element 43.
  • An optical system 44 is constructed between the light source 42 and the sensor cartridge 32 and between the sensor cartridge 32 and the light receiving element 43.
  • the optical system 44 forms an optical path between the light source 42 and the sensor cartridge 32, and at the same time, the light of the light source 42 is guided to the sensor cartridge 32 by the action of the sensor cartridge 32 and the light receiving element 43.
  • the reflected light of the sensor cartridge 32 is guided to the light receiving element 43 by the action of the optical system 44.
  • the optical system 44 includes a collimator lens 45, a dichroic mirror 46, an objective lens 47, a condenser lens 48, a concave lens 49, an optical filter 51, and a spectroscope 52.
  • the dichroic mirror 46 is disposed between the sensor cartridge 32 and the light receiving element 43, for example.
  • the objective lens 47 is disposed between the dichroic mirror 46 and the sensor cartridge 32.
  • the objective lens 47 collects the parallel light supplied from the dichroic mirror 46 and guides it to the sensor cartridge 32.
  • the reflected light of the sensor cartridge 32 is converted into parallel light by the objective lens 47 and transmitted through the dichroic mirror 46.
  • a condensing lens 48, a concave lens 49, an optical filter 51, and a spectroscope 52 are disposed between the dichroic mirror 46 and the light receiving element 43.
  • the optical axes of the objective lens 47, the condensing lens 48, and the concave lens 49 are coaxially adjusted.
  • the light condensed by the condenser lens 48 is converted again into parallel light by the concave lens 49.
  • the optical filter 51 removes Rayleigh scattered light.
  • the Raman scattered light passes through the optical filter 51.
  • the spectroscope 52 selectively transmits light having a specific wavelength.
  • the light receiving element 43 detects the light intensity for each specific wavelength.
  • an etalon can be used for the spectroscope 52.
  • the optical axis of the light source 42 is orthogonal to the optical axes of the objective lens 47 and the condenser lens 48.
  • the surface of the dichroic mirror 46 intersects these optical axes at an angle of 45 degrees.
  • a collimator lens 45 is disposed between the dichroic mirror 46 and the light source 42. Thus, the collimator lens 45 is opposed to the light source 42.
  • the optical axis of the collimator lens 45 is coaxially aligned with the optical axis of the light source 42.
  • the target molecule detection device 31 includes a control unit 53.
  • the control unit 53 is connected to the light source 42, the spectroscope 52, the light receiving element 43, the suction unit 38, and other devices.
  • the control unit 53 controls the operation of the light source 42, the spectroscope 52, and the suction unit 38 and processes the output signal of the light receiving element 43.
  • a signal connector 54 is connected to the control unit 53.
  • the control unit 53 can exchange signals with the outside through the signal connector 54.
  • the target molecule detection device 31 includes a power supply unit 55.
  • the power supply unit 55 is connected to the control unit 53.
  • the power supply unit 55 supplies operating power to the control unit 53.
  • the control unit 53 can operate by receiving power from the power supply unit 55.
  • a primary battery or a secondary battery can be used.
  • the secondary battery can have a power connector 56 for charging, for example.
  • the control unit 53 includes a signal processing control unit.
  • the signal processing control unit can be constituted by, for example, a central processing unit (CPU) and a storage circuit such as a RAM (Random Access Memory) and a ROM (Read Only Memory).
  • a processing program and spectrum data can be stored in the ROM.
  • the spectrum data identifies the Raman scattered light spectrum of the target molecule.
  • the CPU executes the processing program while temporarily fetching the processing program and spectrum data into the RAM.
  • the CPU compares the spectrum data with the spectrum of light specified by the action of the spectroscope and the light receiving element.
  • the sensor cartridge 32 includes a housing 58.
  • the casing 58 partitions the detection chamber 59.
  • the detection chamber 59 is connected to the introduction passage 33 at one end and to the discharge passage 34 at the other end.
  • the sensor chip 11 is incorporated in the housing 58.
  • the surface of the substrate 12 is in contact with the space of the detection chamber 59.
  • Light emitted from the light source 42 is converted into parallel light by the collimator lens 45.
  • the linearly polarized light is reflected by the dichroic mirror 46.
  • the reflected light is collected by the objective lens 47 and applied to the sensor cartridge 32. At this time, the light can be incident in a vertical direction orthogonal to the surface of the sensor chip 11. So-called normal incidence can be established.
  • the plane of polarization of light is aligned in parallel with the linear uneven pattern 19 (second direction SD).
  • Localized surface plasmon resonance is caused in the metal nanobody 23 by the action of the irradiated light.
  • Near-field light is intensified between the metal nanobodies 23. A so-called hot spot is formed.
  • the light emitted from the sensor cartridge 32 is converted into parallel light by the objective lens 47 and passes through the dichroic mirror 46, the condensing lens 48, the concave lens 49 and the optical filter 51.
  • the Raman scattered light is incident on the spectroscope 52.
  • the spectroscope 52 separates the Raman scattered light.
  • the light receiving element 43 detects the light intensity for each specific wavelength.
  • the spectrum of light is checked against the spectral data.
  • the target molecule can be detected.
  • the target molecule detection device 31 can detect a target substance such as adenovirus, rhinovirus, HIV virus, or influenza virus based on the surface enhanced Raman scattering.

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PCT/JP2013/002922 2012-05-10 2013-05-02 センサーチップ並びにセンサーカートリッジおよび検出装置 Ceased WO2013168401A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP13787635.5A EP2848920A4 (en) 2012-05-10 2013-05-02 SENSOR CHIP, SENSOR CARTRIDGE AND DETECTION DEVICE
CN201380024597.7A CN104303046A (zh) 2012-05-10 2013-05-02 传感器芯片、传感器盒及检测装置
US14/400,049 US20150098085A1 (en) 2012-05-10 2013-05-02 Sensor chip, sensor cartridge, and detection apparatus

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