WO2013152699A1 - 推片染色机染色装置及推片染色机的染色方法 - Google Patents

推片染色机染色装置及推片染色机的染色方法 Download PDF

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Publication number
WO2013152699A1
WO2013152699A1 PCT/CN2013/073892 CN2013073892W WO2013152699A1 WO 2013152699 A1 WO2013152699 A1 WO 2013152699A1 CN 2013073892 W CN2013073892 W CN 2013073892W WO 2013152699 A1 WO2013152699 A1 WO 2013152699A1
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WIPO (PCT)
Prior art keywords
dyeing
blocking
separating
tank
pool
Prior art date
Application number
PCT/CN2013/073892
Other languages
English (en)
French (fr)
Inventor
南永涛
李为公
陈良清
董丽芳
Original Assignee
深圳迈瑞生物医疗电子股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 深圳迈瑞生物医疗电子股份有限公司 filed Critical 深圳迈瑞生物医疗电子股份有限公司
Publication of WO2013152699A1 publication Critical patent/WO2013152699A1/zh
Priority to US14/512,226 priority Critical patent/US9612180B2/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N2001/315—Basket-type carriers for tissues

Definitions

  • the invention relates to a medical instrument, in particular to a dyeing device for a pusher dyeing machine and a dyeing method for a pusher dyeing machine.
  • Multi-slice dyeing technique multiple-slice dyeing is to put a plurality of prepared smears into a basket-like device, immerse the device containing multiple smears in the dyeing tank for dyeing, and dye according to the prescribed process. The smear is taken out and the next process is carried out, and the dye solution in the dyeing tank can be reused. Due to the repeated use of the dyeing solution, the quality of the dyeing liquid is inevitably lowered, so that the dyeing effect of different batches of dyed smears cannot be guaranteed from the quality, and the diagnosis of the relevant clinical pathology is easily affected.
  • Single-chip dyeing is to dye the prepared smear piece by a separate dyeing tank.
  • the dyeing pool space is as small as possible, and only one smear can be accommodated to reduce the dyeing pool.
  • the amount of dyeing liquid in it When the amount of smear to be dyed is small, the reagent consumption is lower than that of multiple pieces of dyeing. When the amount of smear is large, although the amount of dyeing solution is equivalent to that of multiple pieces of dyeing technology, the quality is excellent. Multi-slice dyeing technology.
  • the dyeing pool of the conventional single-chip dyeing machine cannot be automatically recycled, resulting in waste of dyeing pool resources and low work efficiency.
  • a pusher dyeing machine dyeing device comprising:
  • the work position is set on the closed transfer channel, and the work bits include:
  • a blocking and separating mechanism for blocking the dyeing tank to be treated in the working position and separating the treated dyeing tank to transfer the separated dyeing tank to the next working position along the closed conveying channel under the conveying of the conveying mechanism;
  • a reagent supply and discharge mechanism for injecting reagent into the dyeing tank and/or discharging the reagent in the dyeing tank;
  • a drive mechanism for driving the blocking and separating mechanism is provided.
  • the transport mechanism includes a disk driven by a motor having a ring-shaped opening for suspending the dyeing cell.
  • the dyeing tank comprises a body, the body is provided with an open cavity for accommodating the reagent and the smear, and the two sides of the body are provided with a hanging end for suspending the dyeing pool on the conveying mechanism, and the hanging end is provided with a blocking and separating mechanism The slot for separation.
  • the blocking and separating mechanism includes a pawl arm coupled to the drive mechanism, the pawl arm being moved by a drive mechanism, the pawl arm including a blocking portion, a separating portion, and a connecting portion that secures the connecting blocking portion and the separating portion
  • the blocking portion and the separating portion are respectively located in the closed conveying passage of the dyeing tank under the driving of the driving mechanism, and the positions of the blocking portion and the separating portion in the passage are arranged before and after the conveying mechanism transmits the dyeing pool.
  • the number of the claw arms is two, and the blocking portion and the separating portion of the same claw arm are disposed on both sides of the target dyeing pool, and the blocking portions of the two claw arms are disposed in front of the conveying direction of the target dyeing pool.
  • the separation portions of the two claw arms are disposed behind the conveying direction of the target dyeing pool.
  • each of the work stations further includes detecting means for detecting the position of the dyeing tank, the detecting means is coupled to the driving mechanism, and the dyeing pool is further provided with a position detecting piece for detecting.
  • a dyeing method for a pusher dyeing machine comprising the following steps:
  • the treated dyeing tank is separated and transferred along the closed conveying channel to the next working position.
  • the above treatment is to block at least one of a predetermined time, injecting a reagent into the dyeing tank, and discharging a reagent in the dyeing tank.
  • the closed transfer channel is a circular channel.
  • the step of blocking the dyeing tank to be treated in the working position is: driving the blocking portion of the claw arm to place the blocking portion in front of the closed conveying passage of the dyeing tank; separating the treated dyeing pool
  • the direction of the pool is set before and after.
  • the method further comprises the steps of first injecting the dye liquor into the dyeing tank and then placing the smear to be dyed.
  • the above-mentioned pusher dyeing machine dyeing device and dyeing method enable the dyeing tank to be continuously transported in a closed conveying passage, the working position blocks and separates the dyeing tank, and the dyeing liquid or the cleaning liquid is injected and discharged into the dyeing tank as needed, so that each The dyeing pool can be recycled and used to improve work efficiency.
  • FIG. 1 is a schematic top plan view of a dyeing device for a pusher dyeing machine according to an embodiment
  • FIG. 2 is a schematic structural view of a blocking and separating mechanism
  • Figure 3 is a schematic view showing the structure of the blocking and separating mechanism at another angle
  • Figure 4 is a schematic view showing the structure of the front side of the blocking and separating mechanism. The state shown in the figure is that the blocking portion is in the state of blocking the dyed sheet, and the separating portion is separated from the state in which the dyed sheet is in contact;
  • Figure 5 is a schematic view showing the operation of the blocking and separating mechanism in the state shown in Figure 5;
  • Figure 6 is a schematic view showing the structure of the front side of the blocking and separating mechanism. The state shown in the figure is that the separating portion contacts the dyed sheet, and the blocking portion is separated from the state in which the dyed sheet is in contact;
  • Figure 7 is a schematic view showing the operation of the blocking and separating mechanism in the state shown in Figure 7;
  • Figure 8 is a schematic view showing the front structure of a single-piece dyeing tank
  • Figure 9 is a top plan view of a single piece dyeing cell.
  • the pusher dyeing machine dyeing apparatus includes a plurality of dyeing tanks 20, a conveying mechanism 100, and a working position 600.
  • a plurality of dyeing cells 20 are suspended from the transport mechanism 100, and the transport mechanism 100 circulates the monolithic dye pool 20 on the closed transport path.
  • the work station 600 is disposed on the closed transfer passage, including the blocking and separating mechanism 30, the reagent supply and discharge mechanism 40, and the drive mechanism 200.
  • the function of the blocking and separating mechanism 30 is to block the dyeing tank 20 to be treated in the working position, to separate the treated dyeing tank 20, and to transfer the separated dyeing tank 20 to the lower conveying channel under the conveyance of the conveying mechanism 100.
  • a job location is to block the dyeing tank 20 to be treated in the working position, to separate the treated dyeing tank 20, and to transfer the separated dyeing tank 20 to the lower conveying channel under the conveyance of the conveying mechanism 100.
  • the reagent supply and discharge mechanism 40 injects reagents into the dyeing tank and/or discharges the reagents from the dyeing tank as needed.
  • the drive mechanism 200 drives the blocking and separating mechanism 30.
  • the pusher dyeing machine dyeing device continuously keeps the dyeing tank 20 in a closed conveying passage, and the working position can block and separate the dyeing tank 20 as needed, and inject the reagent into the dyeing tank 20 or discharge the reagent from the dyeing tank as needed.
  • the use of the dyeing pool can be recycled, which improves work efficiency.
  • the dyeing tank 20 is no longer involved in the loading and loading of other working processes of the dyeing machine, and the dyeing liquid can be injected into the dyeing tank 20 at the beginning of the dyeing process. Then, the smear 60 to be dyed is placed, so that the risk of hemolysis is completely avoided, and the quality of the dyeing is ensured.
  • the dyeing tank 20 is suspended from the conveying mechanism 100, so that the dyeing tank 20 can be driven by the conveying mechanism 100 by the contact friction with the conveying mechanism 100 during the conveying.
  • the dyeing tank 20 rotates cyclically with the conveying mechanism 100.
  • the external force is greater than the contact friction between the dyeing tank 20 and the conveying mechanism 100, the dyeing tank 20 will be blocked from being placed at the external force application, that is, relative The transport mechanism 100 slides.
  • the external force disappears, the dyeing tank 20 will continue to be transported by the transport mechanism 100.
  • the blocking and separating mechanism 30 of each working position of the pusher dyeing machine dyeing device realizes the blocking and separation of the dyeing pool based on the principle.
  • the smear 60 to be dyed can be placed into the blocked dyeing tank 20 until the blocking and separating mechanism 30 releases the blocked dyeing tank 20, and the released dyeing tank 20 is conveyed.
  • the mechanism 100 rotates together to enter the next work position.
  • the single-piece dyeing tank includes a body 21 provided with an open cavity 22 containing a reagent and a smear 60, the body 21 On both sides, a suspension end 23 for suspending the dyeing tank 20 from the conveying mechanism 100 is provided, and the hanging end 23 is provided with a slot 24 for separating the separating and separating mechanism 30.
  • the hanging end 23 may be disposed on both sides of the upper portion of the body 21.
  • the transport mechanism 100 includes a motor 111 and a disk 110 that is driven by a motor 111.
  • the motor 111 drives the disk 110 to rotate counterclockwise.
  • the disk 110 is provided with a closed conveying passage formed by an annular opening 112 for suspending the single-piece dyeing tank; of course, the disk can also be rotated clockwise.
  • the closed conveying passage of the conveying mechanism 100 may be of other forms or shapes as long as the dyeing tank 20 can be circulated, for example, two parallel straight sides similar to a stadium track are connected by two semicircles.
  • the blocking and separating mechanism 30 includes a pawl arm 300 coupled to the drive mechanism 200 that is moved by the drive mechanism 200.
  • the claw arm 300 includes a blocking portion 310, a separating portion 320, and a connecting portion 330 that fixes the connecting blocking portion 310 and the separating portion 320.
  • the blocking portion 310 and the separating portion 320 are respectively located in the closed conveying channel of the dyeing pool under the driving of the driving mechanism 200. And the position of the blocking portion 310 and the separating portion 320 in the passage is disposed before and after the conveying mechanism 100 transmits the dyeing pool 20 in the direction.
  • the blocking portion 310 and the separating portion 320 are respectively driven by the driving mechanism 200, respectively, in the channel in which the conveying mechanism 100 conveys the dyeing tank, and the blocking portion 310 and the separating portion 320 are conveyed along the conveying mechanism 100 at the position in the channel.
  • the direction is disposed before and after, that is, the blocking portion 310 may first block the dyeing pool 20 in the channel for a predetermined time.
  • the blocking portion 310 moves out of the channel and the separating portion 320 moves into the channel, the separating portion 320 is inserted into the forefront dyeing pool 20 due to the existence of the interval.
  • the dyeing pool behind the foremost dyeing tank 20 is blocked.
  • the blocking portion 310 and the separating portion 320 are fixedly coupled integrally by the connecting portion 330, and the interval can be ensured to be fixed, thereby avoiding the fitting error of the blocking portion 310 and the separating portion 320.
  • the number of the claw arms 300 is two, the blocking portion 310 and the separating portion 320 of the same claw arm are disposed on both sides of the target dyeing pool 20, and the blocking portions 310 of the two claw arms 300 are disposed on the target.
  • the separating portions 320 of the two claw arms 300 are disposed rearward in the conveying direction of the target dyeing tank.
  • the number of claw arms may also be one.
  • the two claw arms provide more stable blocking and separation.
  • the number of claw arms may be three, four, or the like.
  • the claw arm 300 includes a first claw arm 300a and a second claw arm 300b. Both the first claw arm 300a and the second claw arm 300b can linearly reciprocate.
  • the direction of movement of the first claw arm 300a and the second claw arm 300b is perpendicular to the target dyeing cell conveying direction. Specifically, the first claw arm 300a and the second claw arm 300b linearly reciprocate in the radial direction of the conveying mechanism.
  • the first claw arm 300a includes a first blocking portion 310a and a first separating portion 320a.
  • the second claw arm 300b includes a second blocking portion 310b and a second separating portion 320b.
  • the first blocking portion 310a and the first separating portion 320a are disposed at positions on both sides of the target dyeing pool 20.
  • the second blocking portion 310b and the second separating portion 320b are disposed at positions on both sides of the target dyeing pool 20.
  • the first blocking portion 310a and the second blocking portion 310b are both disposed in front of the conveying direction of the target dyeing pool 20.
  • the first separating portion 320a and the second separating portion 320b are both disposed behind the conveying direction of the target dyeing pool 20.
  • a plurality of dyeing cells 20 are transported by the transport mechanism 100.
  • the first blocking portion 310a and the second blocking portion 310b are both located in the passage of the conveying mechanism 100 for conveying the dyeing pool
  • the first separating portion 320a and the second separating portion 320b are both located outside the passage of the conveying mechanism 100 for conveying the dyeing pool ( As shown in Figure 4 and Figure 5).
  • the first blocking portion 310a and the second blocking portion 310b are respectively adjacent to the dyeing pool 20 from both sides, and are blocked in front of the conveying direction of the dyeing tank. Thereby the dyeing tank 20 is stopped.
  • the driving mechanism drives the first claw arm 300a to move to the outside of the conveying mechanism 100, and drives the second claw arm 300b to the inner side of the conveying mechanism 100.
  • the first blocking portion 310a and the second blocking portion 310b are away from the dyeing bath 20, and the resistance of the first dyeing pool 20 is eliminated, and is driven by the conveying mechanism 100.
  • the first separating portion 320a and the second separating portion 320b enter the channel of the conveying mechanism 100 for conveying the dyeing pool 20, and are caught between the first dyeing pool and the second dyeing pool, blocking the movement of the second dyeing pool, thereby realizing The separation of the first dye pool from the second dye pool.
  • the driving mechanism 200 drives the first claw arm 300a to move to the inside of the conveying mechanism 100, and drives the second claw arm 300b to move to the outside of the conveying mechanism 100.
  • the first blocking portion 310a and the second blocking portion 310b are returned to the passage of the conveying mechanism 100 for conveying the dyeing tank 20, and the first separating portion 320a and the second separating portion 320b are retracted to the outside of the passage of the conveying mechanism 100 for conveying the dyeing pool 20 (as shown in the figure). 4 and Figure 5).
  • the second dyeing pool is released by the first separating portion 320a and the second separating portion 320 to be moved by the conveying mechanism 100, but when reaching the position of the first blocking portion 310a and the second blocking portion 310b, the first blocking portion The 310a and the second blocking portion 310b are blocked in front of the conveying direction of the second dyeing tank 20, thereby stopping the second dyeing tank and maintaining until the dyeing is completed. This cycle allows for continuous operation.
  • the blocking and separating actions of blocking and separating are realized by the linear reciprocating motion of the first claw arm 300a and the second claw arm 300b, and the linear reciprocating opening and closing action is for the sake of simplicity.
  • the quick-moving blocking portion 310 and the separating portion 320 achieve the purpose of alternately blocking the dyeing pool 20.
  • the first claw arm and the second claw arm may be rotated around the hinge, and blocking and separation may also be achieved by rotating around the hinge.
  • the first claw arm and the second claw arm may also be other movement modes, as long as the claw arm driving blocking portion and the separating portion can alternately block the dyeing pool.
  • the present embodiment is provided with a first claw arm 300a and a second claw arm 300b, so that the first blocking portion 310a and the second blocking portion 310b can be simultaneously blocked from the sides of the dyeing pool 20, respectively.
  • the dyeing pool 20 is uniformly balanced against the conveying direction, so that the dyeing pool is not caused. 20 produces a positional offset with respect to the transport mechanism 100.
  • the working conditions allow for the influence of the offset of the blocking portion and the separating portion on the transfer position of the dyeing tank, the setting of the claw arm can be otherwise.
  • a separating portion and a blocking portion are installed on the claw arm, and the separating portion and the blocking portion are disposed on both sides of the dyeing pool, and the widths of the separating portion and the blocking portion are appropriately increased to maintain stability, and the claw can also be realized.
  • the separation and blocking actions are staggered.
  • the blocking portion 310 is provided with an adsorption portion 312 on one side of the dyeing cell conveying direction, and the adsorption portion 312 is provided with a magnetic material.
  • the adsorption portion 312 can be adsorbed to the portion of the dyeing pool 20 where iron or magnetic is present, so that the blocking portion 310 can better block the dyeing pool 20 stably.
  • the separation portion 320 extends out of the separation pin 322 that can be inserted between two adjacent dyeing cells.
  • the separating portion 320 can perform a separating operation by inserting the separating pin 322 between the two adjacent dyeing cells 20.
  • Absorbing portion of course, in a further preferred embodiment, the separating pin 322 can be detachably fixed to the separating portion 320, so that when the separating pin 322 is worn or the like due to repeated operations, the new separating pin 322 can be removed and replaced, which further Improve work reliability and working life.
  • each of the working positions 600 further includes detecting means 70 for detecting the position of the dyeing tank, and the detecting means 70 is connected to the driving mechanism 200 for driving the claw arms 300. .
  • the present dyeing device can precisely control the blocking and separating actions, as well as the injection and discharge actions of the reagent supply and discharge mechanism 40 to the reagents.
  • the detection device 70 can be an optocoupler-detecting device 70 that is mounted to the side mount 400 of the blocking and separating mechanism 30.
  • other types of detecting devices 70 can be used as long as the position signal of the dyeing pool 20 is collected and output to the driving mechanism 200. This has an alternative in the field of position detecting technology, and will not be further described herein.
  • the single-piece dyeing pool is further provided with a position detecting sheet 25 for detection.
  • the detecting device 70 uses the detecting optocoupler detecting device 70, when the dyeing cell 20 is transported by the transport mechanism 100 to the position where the detecting device 70 is located, the position detecting sheet 25 is inserted into the detecting optocoupler, thereby The detection optocoupler is acquired with a signal and transmitted to the drive mechanism 200 so that the drive mechanism 200 controls the pawl arm 300 to operate.
  • the detecting device 70 can be an optical coupling type detecting device 70 that is mounted to the side mount 400 of the pusher dyeing device.
  • other types of detecting devices 70 can be used as long as the position signal of the dyeing pool 20 is collected and output to the driving control mechanism. This has an alternative in the field of position detecting technology, and will not be further described herein.
  • the ends of the jaw arms 300 are movably sleeved in the guiding mechanism.
  • the guiding mechanism includes a first guide post 410 mounted to the side mount 400, and a second guide post 510 mounted to the center mount 500 of the pusher dyeing device.
  • the number of the first guide post 410 and the second guide post 510 may be one, two, or the like.
  • the end of the claw arm 300 is provided with a matching guide hole at a position corresponding to the first guide post 410 and the second guide post 510, and the end of the claw arm 300 is slidable along the first guide post 410 and the second guide post 510 through the guide hole.
  • the axial direction of the first guiding post 410 and the second guiding post 510 coincides with the moving direction of the end of the claw arm 300. This enhances the structural strength of the end of the pawl arm 300 while not affecting the movement of the pawl arm 300 to achieve the separation and blocking action.
  • the end of the claw arm 300 may be one or a combination of one or more of the first blocking portion 310a, the second blocking portion 310b, the first separating portion 320a, and the second separating portion 320b.
  • the pusher dyeing machine dyeing pool blocking and separating device further includes a position adjusting mechanism, and the guiding mechanism is disposed on the position adjusting mechanism.
  • the position adjustment mechanism includes a guide block 430 disposed in the guide groove 420 of the side mount 400.
  • the guiding block 430 is slidably disposed on the guiding slot 420.
  • the first guiding post 410 in the guiding mechanism is fixed on the guiding block 430.
  • the guiding block 430 can be slid along the guiding slot 420, thereby The pawl arm 300 is adjusted to a suitable position by a guiding mechanism.
  • the position of the guide block 430 in the guide groove 420 is defined to fix the guide mechanism.
  • the hanging end of the dyeing pool is provided with the slot 24, and when the blocking and separating mechanism 30 is operated, the separating portion 320 can be smoothly inserted between the two adjacent dyeing pools 20, thereby avoiding the direct impact dyeing of the separating portion 320.
  • the body 21 of the pool 20 improves the operational reliability.
  • the blocking and separating mechanism 30 of the first working position 600 a blocks the front plurality of empty dyeing cells 20 , and the reagent feeding and discharging mechanism 40 first injects the first operation.
  • the reagent required for the empty staining tank 20 at the position (hereinafter referred to simply as the A reagent) is then placed in the above-described empty staining tank 20 with the smear 60 to be dyed.
  • the blocking and separating mechanism 30 separates the dyeing tank 20 containing the smear 60 from other dyed cells without smears and passes it through the first working position 600a, which is dyed with the smear 60.
  • the pool 20 is rotated with the smear 20 with the transport mechanism 100 until it is blocked by the blocking and separating mechanism 30 of the next working position.
  • the blocking and separating mechanism 30 fixes the dyeing tank 20 carrying the smear 60 at the reagent supply and discharge mechanism 40 corresponding to the position, and passes The detecting device 70 at this point detects and confirms the presence of the dyeing pool.
  • the reagent supply and discharge mechanism 40 is inserted into the dyeing tank 20 to remove the A reagent in the dyeing tank, and inject the required other A reagent (hereinafter referred to as B reagent), then the blocking and separating mechanism 30 blocks the subsequent dyeing tank of the second working position 600b and passes the dyeing tank 20 into which the B reagent has been injected, and the dyeing tank 20 rotates with the conveying mechanism. Go to the next block.
  • the blocking and separating mechanism 30 fixes the dyeing tank 20 carrying the smear 60 at the reagent supply and discharge mechanism 40 corresponding to the position, and passes The detecting device 70 at this point detects and confirms the presence of the dyeing pool 20.
  • the reagent supply and discharge mechanism 40 is inserted into the dyeing tank 20 to remove the B reagent in the dyeing tank, and inject the required other A reagent (hereinafter referred to as C reagent), then the blocking and separating mechanism 30 blocks the subsequent dyeing tank of the third working position 600c and passes the dyeing tank 20 into which the liquid C has been injected, and the dyeing tank 20 will rotate with the conveying mechanism.
  • C reagent A reagent
  • the blocking and separating mechanism 30 fixes the dyeing tank 20 carrying the smear 60 at the reagent supply and discharge mechanism 40 corresponding to the position, and passes The detecting device 70 at this point detects and confirms the presence of the dyeing pool 20.
  • the reagent supply and discharge mechanism 40 is inserted into the dyeing tank 20 to remove the C reagent in the dyeing tank, and the washing water is injected for cleaning.
  • the smear 60 is taken away and proceeds to a subsequent process; then the blocking and separating mechanism 30 blocks the subsequent dyeing tank 20 of the fourth working position 600d and allows the empty dyeing tank to pass, and the dyeing tank 20 follows the conveying mechanism. 100 rotate to the area where the dyeing tank is stored and wait for the next cycle.
  • each of the work positions of the present embodiment can be individually operated, and the plurality of smears are simultaneously dyed in parallel.
  • the embodiment is not limited to four work positions, and may be two, three, five, and the like.
  • the relative angle between the work positions can also be optimized according to the dyeing process commonly used by the user.
  • the above treatment is at least one of blocking a predetermined time, injecting a reagent into the dyeing tank, and discharging the reagent in the dyeing tank.
  • the above-mentioned pusher dyeing machine dyeing device can be used as a separate product, and the dyeing tank can be used as a part of the dyeing device of the pusher dyeing machine, and the dyeing tank can also be used as an external component in the working process of the dyeing device of the pusher dyeing machine.
  • a dyeing method for a pusher dyeing machine comprising the steps of: driving a conveying mechanism to move, circulating a dyeing tank on a closed conveying passage; blocking a dyeing tank to be treated in the working position; The dyeing tank of the position is processed; the treated dyeing tank is separated and transferred to the next working position along the closed conveying channel.
  • the treatment is to block at least one of a predetermined time, injecting a reagent into the dye bath, and discharging a reagent in the dye bath. That is to say, it is possible to block only a predetermined period of time without injecting or discharging the reagent, or to inject only the reagent or the discharge reagent, and to discharge both the reagent and the reagent while blocking for a predetermined period of time.
  • the closed conveying passage is a circular passage.
  • the step of blocking the dyeing tank to be treated in the working position is: driving the blocking portion of the claw arm to place the blocking portion in front of the closed conveying passage of the dyeing tank
  • the step of separating the treated dyeing tank is: driving the blocking portion of the claw arm away from the closed conveying passage of the dyeing tank while the separating portion of the driving claw arm is placed in the closed conveying passage of the dyeing tank, and the blocking portion and the separating portion are The conveying mechanism at the position along the channel conveys the direction of the dyeing pool before and after.
  • the dye solution is injected into the dyeing tank at the beginning of the dyeing process, and then the smear to be dyed is placed, thus completely avoiding the risk of hemolysis and ensuring the quality of the dyeing.
  • the dyeing method of the above-mentioned pusher dyeing machine is easier to understand in combination with the structure and working process of the above-mentioned pusher dyeing machine, and will not be described in detail.

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Abstract

一种推片染色机染色装置，包括染色池（20）、传送机构（100）及设置在传送机构的封闭传送通道上的作业位（600，600a，600b，600c，600d）。所述作业位包括：阻挡与分离机构（30），用于将待在本作业位处理的染色池阻挡并将处理后的染色池分离使分离后的所述染色池在所述传送机构的传送下沿所述封闭传送通道传送至下一个作业位；试剂给排机构（40），用于向染色池内注入试剂和/或排出染色池内的试剂；驱动机构（200），用于驱动阻挡与分离机构。上述推片染色机染色装置使染色池始终保持在一个封闭传送通道中循环传送，作业位阻挡和分离染色池，并且根据需要向染色池注入、排出染色液或清洗液，使每个染色池都可以循环的使用，提高了工作效率。此外，还提供了一种推片染色机的染色方法。

Description

推片染色机染色装置及推片染色机的染色方法 技术领域
本发明涉及医疗仪器，特别是涉及一种推片染色机染色装置及推片染色机的染色方法。
背景技术
在传统的自动化血涂片或其他组织涂片的染色装置中，常用的有两大类技术，即多片染色和单片染色技术。
多片染色技术：多片染色是将多个已经准备好的涂片放在类似篮子的装置中，把盛有多片涂片的装置浸在染色池中进行染色，按照规定的流程染色后，把涂片取出，进行下一个流程，而染色池中的染液可以重复使用。由于染液的多次重复使用，染液质量必然下降，因而就无法从质量上保证不同批次染色涂片的染色效果，容易影响对有关临床病理的诊断。另外，在很多实际使用中，往往由于急诊或其他的原因，又需要及时地对几片涂片进行染色，这样就会使用于多片染色的染色液仅用于单片或几片染色，这样一来，就大大地降低了染色液的有效使用率，造成了大量的浪费，使单片染色的成本大大增加。
单片染色技术：单片染色是通过一个个单独的染色池对已经准备好的涂片一片一片的进行染色，通过设计使染色池空间尽量的小，仅能容纳一片涂片，以减少染色池中的染色液量。需染色的涂片量少的时候，比多片染色时的试剂耗量要低，涂片量大的时候虽然染色液的用量与多片染色技术的耗液量相当，但是其质量却明显优于多片染色技术。
然而，传统的单片染色机的染色池不能自动循环使用，造成染色池资源的浪费，并且工作效率低。
发明内容
基于此，有必要针对传统的单片染色机不能自动循环使用的问题，提供一种推片染色机染色装置。
一种推片染色机染色装置，包括：
染色池；
传送机构，用于在封闭传送通道上循环传送染色池；
作业位，设置在封闭传送通道上，作业位包括：
阻挡与分离机构，用于将待在本作业位处理的染色池阻挡并将处理后的染色池分离使分离后的染色池在传送机构的传送下沿封闭传送通道传送至下一个作业位；
试剂给排机构，用于向染色池内注入试剂和/或排出染色池内的试剂；
驱动机构，用于驱动阻挡与分离机构。
在其中一个实施例中，传送机构包括由电机驱动的圆盘，圆盘上设有一圈用于悬挂染色池的环状开口。
在其中一个实施例中，染色池包括本体，本体设置有容纳试剂及涂片的开口腔体，本体两侧设有将染色池悬挂于传送机构的悬挂端，悬挂端开设有供阻挡与分离机构分离用的插槽。
在其中一个实施例中，阻挡与分离机构包括与驱动机构相连的爪臂，该爪臂在驱动机构的驱动下移动，爪臂包括阻挡部、分离部以及固定连接阻挡部和分离部的连接部，阻挡部和分离部在驱动机构的驱动下分别依次位于染色池的封闭传送通道内，且阻挡部和分离部在通道内的位置沿传送机构传送染色池的方向前后设置。
在其中一个实施例中，爪臂的数量为两个，同一爪臂的阻挡部和分离部设于目标染色池的两侧，两个爪臂的阻挡部均设置于目标染色池传送方向的前方，两个爪臂的分离部均设置于目标染色池传送方向的后方。
在其中一个实施例中，每个作业位还包括用于检测染色池位置的检测装置，检测装置与驱动机构连接，染色池还设置有用于检测的位置检测片。
一种推片染色机的染色方法，包括如下步骤：
驱动传送机构移动，在封闭传送通道上循环传送染色池；
将待在本作业位处理的染色池阻挡；
对阻挡在本作业位的染色池进行处理；
将处理后的染色池分离并沿封闭传送通道传送至下一作业位。
在其中一个实施例中，上述处理为阻挡预定时间、向染色池内注入试剂和排出染色池内的试剂中的至少一种。
在其中一个实施例中，封闭传送通道为圆形通道。
在其中一个实施例中，将待在本作业位处理的染色池阻挡的步骤为：驱动爪臂的阻挡部将阻挡部置于染色池的封闭传送通道内的前方；将处理后的染色池分离的步骤为：驱动爪臂的阻挡部离开染色池的封闭传送通道的同时驱动爪臂的分离部置于染色池的封闭传送通道内，阻挡部和分离部在通道内的位置沿传送机构传送染色池的方向前后设置。
在其中一个实施例中，还包括先向染色池内注入染液，再放入需要染色的涂片的步骤
上述推片染色机染色装置及染色方法使染色池始终保持在一个封闭传送通道中循环传送，作业位阻挡和分离染色池，并且根据需要向染色池注入、排出染色液或清洗液，使每个染色池都可以循环的使用，提高了工作效率。
附图说明
图1为一实施例的推片染色机染色装置的俯视结构示意图；
图2为阻挡与分离机构的结构示意图；
图3为另一角度的阻挡与分离机构的结构示意图；
图4为阻挡与分离机构的正面结构示意图，本图所示的状态为阻挡部处于阻挡染色片，而分离部脱离于染色片接触的状态；
图5为阻挡与分离机构处于图5所示状态时的工作示意图；
图6为阻挡与分离机构的正面结构示意图，本图所示的状态为分离部接触染色片，而阻挡部脱离于染色片接触的状态；
图7为阻挡与分离机构处于图7所示状态时的工作示意图；
图8为单片染色池的正面结构示意图；
图9为单片染色池的俯视图。
具体实施方式
如图1及图2所示，推片染色机染色装置，包括若干染色池20、传送机构100和作业位600。多个染色池20悬挂于传送机构100上，传送机构100在封闭传送通道上循环传送单片染色池20。作业位600设置在封闭传送通道上，包括阻挡与分离机构30、试剂给排机构40以及驱动机构200。阻挡与分离机构30的作用是将待在本作业位处理的染色池20阻挡、将处理后的染色池20分离使分离后的染色池20在传送机构100的传送下沿封闭传送通道传送至下一个作业位。试剂给排机构40根据需要向染色池内注入试剂和/或从染色池中排出试剂。驱动机构200驱动阻挡与分离机构30。推片染色机染色装置使染色池20始终保持在一个封闭传送通道中循环传送，作业位可以根据需要阻挡和分离染色池20，并且根据需要向染色池20注入试剂或从染色池中排出试剂，使染色池可以循环的使用，提高了工作效率。
同时，由于推片染色机染色装置相对独立于染色机的其他机构，因此，染色池20不再参与染色机其他机构工作流程的运送装载，可在染色流程开始时先将染液注入染色池20，然后再放入需要染色的涂片60，这样就完全避免了溶血的风险，保证了染色的质量。
染色池20悬挂于传送机构100上，故而传送过程中，染色池20通过与传送机构100之间的接触摩擦力而可被传送机构100带动。染色池20随着传送机构100循环转动，当有外力施加于染色池20，该外力大于染色池20与传送机构100的接触摩擦力时，染色池20将被阻挡止位于外力施加处，即相对于传送机构100滑动。而该外力消失时，染色池20又将在传送机构100带动下继续被传送。推片染色机染色装置每个作业位的阻挡与分离机构30即是基于该原理实现对染色池的阻挡和分离。当染色池被作业位阻挡时，需要染色的涂片60可以放置到被阻挡的染色池20里，直到阻挡与分离机构30放开被阻挡的染色池20，被放开的染色池20随传送机构100一起转动，进入到下一个作业位。
如图8和图9所示，为了配合阻挡与分离机构30，在具体的实施例中，单片染色池包括本体21，本体21设置有容纳试剂及涂片60的开口腔体22，本体21两侧设有将染色池20悬挂于传送机构100的悬挂端23，悬挂端23开设有供阻挡与分离机构30分离用的插槽24。当然，为了使染色池20在传送机构100上的悬挂更稳定，在具体的实施例中，悬挂端23可以设置于本体21上部两侧。
如图1和图2所示，在具体的实施例中，传送机构100包括电机111及由电机111驱动的圆盘110。电机111驱动圆盘110逆时针旋转，圆盘110上设有一圈用于悬挂单片染色池的环状开口112形成的封闭传送通道；当然圆盘也可以顺时针旋转。此外，传送机构100的封闭传送通道也可以是其他形式或者形状，只要能循环的传送染色池20即可，例如类似于体育场跑道的两条平行直边通过两个半圆相连等。
如图2和图3所示，在具体的实施例中，阻挡与分离机构30包括与驱动机构200相连的爪臂300，该爪臂300在驱动机构200的驱动下移动。爪臂300包括阻挡部310、分离部320以及固定连接阻挡部310和分离部320的连接部330，阻挡部310和分离部320在驱动机构200的驱动下分别依次位于染色池的封闭传送通道内，且阻挡部310和分离部320在通道内的位置沿传送机构100传送染色池20的方向前后设置。
由于阻挡部310和分离部320在驱动机构200的驱动下分别依次位于传送机构100传送染色池的通道内，且阻挡部310和分离部320在通道内的位置沿传送机构100传送染色池20的方向前后设置，即阻挡部310可以先在通道内阻挡染色池20预定时间，待阻挡部310移出通道而分离部320移入通道时，由于间隔的存在，分离部320插入到最前方染色池20的后方，挡住最前方染色池20之后的染色池。阻挡部310和分离部320通过连接部330固定连接成一体，可以确保间隔固定，避免阻挡部310和分离部320出现配合误差。
具体到本实施例中，爪臂300的数量为两个，同一爪臂的阻挡部310和分离部320设于目标染色池20的两侧，两个爪臂300的阻挡部310均设置于目标染色池20传送方向的前方，两个爪臂300的分离部320均设置于目标染色池传送方向的后方。在其他的实施例中，爪臂的数量也可以是一个，当然，两个爪臂能起到更稳定的阻挡和分离。另外，当需要在多个位置实现阻挡和分离时，爪臂的数量也可以是三个、四个等。
对于两个爪臂在一个位置实现阻挡和分离的情况，如图2至图4所示，爪臂300包括第一爪臂300a和第二爪臂300b。第一爪臂300a和第二爪臂300b均可做直线往复运动。第一爪臂300a与第二爪臂300b的运动方向垂直于目标染色池传送方向。具体地说，第一爪臂300a与第二爪臂300b沿传送机构的径向做直线往复运动。
第一爪臂300a包括第一阻挡部310a和第一分离部320a。第二爪臂300b包括第二阻挡部310b和第二分离部320b。第一阻挡部310a和第一分离部320a分设于目标染色池20两侧的位置。第二阻挡部310b和第二分离部320b分设于目标染色池20两侧的位置。第一阻挡部310a和第二阻挡部310b均设置于目标染色池20传送方向前方。第一分离部320a和第二分离部320b均设置于目标染色池20传送方向后方。
请同时参阅图2至图5，工作时，多个染色池20被传送机构100传送。初始状态下，第一阻挡部310a和第二阻挡部310b都位于传送机构100传送染色池的通道内，第一分离部320a与第二分离部320b均位于传送机构100传送染色池的通道外（如图4和图5所示）。当第一个染色池20被传送机构100运送至阻挡部310的位置时，第一阻挡部310a和第二阻挡部310b分别从两侧贴近染色池20，并阻挡于染色池传送方向的前方，从而使该染色池20停止。
如图6和图7所示，第一个染色池完成对涂片的染色后，驱动机构带动第一爪臂300a向传送机构100的外侧移动，带动第二爪臂300b向传送机构100的内侧移动。第一阻挡部310a和第二阻挡部310b远离染色池20，第一个染色池20阻力消除，在传送机构100带动下离开。同时第一分离部320a与第二分离部320b进入到传送机构100传送染色池20的通道内，卡在第一个染色池与第二个染色池之间，阻挡第二个染色池移动，从而实现第一个染色池与第二个染色池的分离。
分离动作完成后，驱动机构200带动第一爪臂300a向传送机构100的内侧移动，带动第二爪臂300b向传送机构100的外侧移动。第一阻挡部310a和第二阻挡部310b回到传送机构100传送染色池20的通道内，第一分离部320a与第二分离部320b退到传送机构100传送染色池20的通道外（如图4和图5所示）。第二个染色池被第一分离部320a与第二分离部320释放而在传送机构100的带动下移动，但是当到达第一阻挡部310a和第二阻挡部310b的位置时，第一阻挡部310a和第二阻挡部310b阻挡于第二个染色池20传送方向的前方，从而使第二个染色池停止，并保持至完成染色。如此循环，可实现连续作业。
应当理解的是，在本实施例中，通过第一爪臂300a与第二爪臂300b的直线往复运动实现阻挡与分离的阻挡和分离动作，这种直线往复运动式的开合动作是为了简便快捷地带动阻挡部310和分离部320实现交替阻挡染色池20的目的。但不限于这种直线往复运动式开合，在另一实施例中，还可以是第一爪臂与第二爪臂绕铰接处转动，通过绕铰接处转动亦可实现阻挡和分离。当然，第一爪臂与第二爪臂也可以是其它的运动方式，只要能实现爪臂带动阻挡部和分离部交替阻挡染色池的目的即可。
还应当理解的是，本实施例设置有第一爪臂300a与第二爪臂300b，这是为了使第一阻挡部310a和第二阻挡部310b可分别从染色池20两侧同步实施阻挡动作，亦是为了使第一分离部320a和第二分离部320b可分别从染色池20两侧同步实施阻挡动作，从而使染色池20两侧均衡地受到与传送方向相反的阻力，不致使染色池20相对于传送机构100产生位置偏移。当然，如果工作条件允许不考虑阻挡部和分离部对染色池传送位置造成偏移的影响，爪臂的设置亦可采用其它方式。如只设置一个爪臂，在该爪臂安装一分离部和一阻挡部，分离部与阻挡部分设于染色池两侧，适当增加分离部和阻挡部的宽度以保持稳定性，亦可实现爪臂移动时交错实施分离与阻挡动作的目的。
请参阅图4或图6，在具体的实施例中，阻挡部310正对染色池传送方向的一面设有吸附部312，吸附部312设有磁性材料。当阻挡部310实施阻挡动作时，可通过吸附部312与染色池20上有铁或者磁性的部位吸附，使阻挡部310更好的把染色池20稳定地阻挡。
还请参阅图4或图6，在具体的实施例中，分离部320延伸出可插入两相邻染色池之间的分离销322。分离部320可通过分离销322插入两相邻染色池20之间而实施分离动作。吸附部当然，在进一步优选的实施例中，分离销322可以可拆卸地固定于分离部320，这样由于反复动作而产生分离销322磨损等状况时，可以拆卸更换新的分离销322，这进一步提高了工作可靠性和工作寿命。
如图3、图5或图7所示，在具体的实施例中，每个作业位600还包括用于检测染色池位置的检测装置70，检测装置70与驱动爪臂300的驱动机构200连接。通过检测装置70，本染色装置可以精确控制阻挡和分离动作，以及试剂给排机构40对试剂的注入和排出动作。
在一个具体的实施例中，检测装置70可以是检测光耦式的检测装置70，该检测装置70安装于阻挡与分离机构30的侧固定架400。当然，也可以是其它类型的检测装置70，只要能实现采集染色池20位置信号并输出给驱动机构200的目的即可，这在位置检测技术领域存在替代方案，这里不再一一赘述。
如图8和图9所示，在本实施方式中，为了配合检测装置70的检测，单片染色池还设置有用于检测的位置检测片25。请参阅图5或图7，若检测装置70采用检测光耦式的检测装置70，则染色池20被传送机构100传送至检测装置70所在的位置时，位置检测片25插入检测光耦，从而使检测光耦采集到信号，并传输给驱动机构200，以便驱动机构200控制爪臂300动作。
在一个具体的实施例中，检测装置70可以是检测光耦式的检测装置70，该检测装置70安装于推片染色机染色装置的侧固定架400。当然，也可以是其它类型的检测装置70，只要能实现采集染色池20位置信号并输出给驱动控制机构的目的即可，这在位置检测技术领域存在替代方案，这里不再一一赘述。
如图2和图3所示的实施例中，爪臂300的端部可活动地套接在导向机构。具体地说，导向机构包括安装于侧固定架400的第一导向柱410、安装于推片染色机染色装置的中心固定架500的第二导向柱510。第一导向柱410和第二导向柱510的数量可以是一根，也可以是两根等。爪臂300端部与第一导向柱410和第二导向柱510对应的位置开设有配合的导孔，爪臂300端部通过导孔可沿第一导向柱410和第二导向柱510滑动。第一导向柱410、第二导向柱510的轴心方向与爪臂300端部移动方向一致。这样在不影响爪臂300移动而实现分离和阻挡动作的同时，增强了爪臂300端部的结构强度。爪臂300的端部可以是第一阻挡部310a、第二阻挡部310b、第一分离部320a和第二分离部320b中的一个或任意两个以上的组合或者全部。
如图2所示，在本实施方式中，推片染色机染色池阻挡和分离装置还包括位置调节机构，导向机构设置在位置调节机构上。具体地，位置调节机构包括设置在侧固定架400的导向槽420内的导向块430。导向块430滑设于导向槽420，导向机构中的第一导向柱410固定在导向块430上，当需要对爪臂300的安装位置进行调整时，可以沿导向槽420滑动导向块430，从而通过导向机构将爪臂300调整至合适位置。完成位置调整后，限定导向块430在导向槽420中的位置，即可将导向机构固定。在本实施方式中，染色池的悬挂端开设有插槽24，则阻挡与分离机构30工作时，分离部320可顺利插入两相邻染色池20之间，从而避免了分离部320直接撞击染色池20的本体21，提高了工作可靠性。
如图1所示，本实施例以四个作业位为例，第一作业位600a的阻挡与分离机构30阻挡着前面的若干个空的染色池20，试剂给排机构40先注入第一作业位处的空染色池20所需的试剂（下面简称为A试剂），之后将需要染色的涂片60放入到上述空染色池20中。当流程需要时，阻挡与分离机构30把装有涂片60的染色池20和其他的未装涂片的染色池分离，并让其通过第一作业位600a，此装有涂片60的染色池20带着涂片20随传送机构100转动，直到被下一个作业位的阻挡与分离机构30阻挡住。
当载有涂片60的染色池20到达第二作业位600b后，阻挡与分离机构30会把载有涂片60的染色池20固定在与该位置对应的试剂给排机构40处，并通过该处的检测装置70，检测和确认到染色池的存在。根据染色流程，涂片60在A试剂染色池20里浸泡了预设的时间后，试剂给排机构40就会插入到染色池20中排走染色池中的A试剂，并注入所需的另一种试剂（以下简称B试剂），然后阻挡与分离机构30会阻挡住第二作业位600b后续的染色池并让已注入B试剂的染色池20通过，染色池20会随着传送机构的转动到达下一个阻挡位。
当载有涂片60的染色池20到达第三作业位600c后，阻挡与分离机构30会把载有涂片60的染色池20固定在与该位置对应的试剂给排机构40处，并通过该处的检测装置70，检测和确认到染色池20的存在。根据染色流程，涂片60在B试剂染色池20里浸泡了预设的时间后，试剂给排机构40就会插入到染色池20中排走染色池里的B试剂，并注入所需的另一种试剂（以下简称C试剂），然后阻挡与分离机构30会阻挡住第三作业位600c后续的染色池并让已注入C液的染色池20通过，染色池20会随着传送机构转动到达下一个阻挡位。
当载有涂片60的染色池20到达第四作业位600d后，阻挡与分离机构30会把载有涂片60的染色池20固定在与该位置对应的试剂给排机构40处，并通过该处的检测装置70，检测和确认到染色池20的存在。根据染色流程，涂片60在C试剂染色池20里浸泡了预设的时间后，试剂给排机构40就会插入到染色池20中排走染色池里的C试剂，注入清洗水进行清洗，清洗完成后涂片60被取走，进入后续的流程；然后阻挡与分离机构30会阻挡住第四作业位600d后续的染色池20并让空的染色池通过，染色池20会随着传送机构100转动到存放染色池的区域，等待下次循环流程。
如上所述，本实施例的每个作业位都可以单独的进行作业，进而并行地对多个涂片同时进行染色。
当然，本实施例并不限于四个作业位，还可以是两个、三个、五个等。在有多个作业位时，作业位之间的相对角度也可以根据使用者常用的染色流程进行优化。在极端情况下，也可以只有一个作业位，在作业位处理完毕后，通过封闭传送通道返回到同一作业位再次进行处理，在其他位置可以由人工或机器插入或取出涂片。上述的处理为阻挡预定时间、向染色池内注入试剂和排出染色池内的试剂中的至少一种。也就是说，可以只阻挡一段预定的时间而不注入或排出试剂，也可以只注入试剂或排出试剂，还可以在阻挡一段预定的时间的同时既排出试剂又注入试剂。
上述的推片染色机染色装置作为一种独立的产品，可以将染色池作为推片染色机染色装置的一部分，也可以将染色池作为推片染色机染色装置工作过程中的外部元件。
此外，还提供一种推片染色机的染色方法，包括如下步骤：驱动传送机构移动，在封闭传送通道上循环传送染色池；将待在本作业位处理的染色池阻挡；对阻挡在本作业位的染色池进行处理；将处理后的染色池分离并沿封闭传送通道传送至下一作业位。
在其中一个实施例中，处理为阻挡预定时间、向染色池内注入试剂和排出染色池内的试剂中的至少一种。也就是说，可以只阻挡一段预定的时间而不注入或排出试剂，也可以只注入试剂或排出试剂，还可以在阻挡一段预定的时间的同时既排出试剂又注入试剂。
在其中一个实施例中，上述推片染色机的染色方法中封闭传送通道为圆形通道。
在其中一个实施例的推片染色机的染色方法中，将待在本作业位处理的染色池阻挡的步骤为：驱动爪臂的阻挡部将阻挡部置于染色池的封闭传送通道内的前方；将处理后的染色池分离的步骤为：驱动爪臂的阻挡部离开染色池的封闭传送通道的同时驱动爪臂的分离部置于染色池的封闭传送通道内，的阻挡部和分离部在通道内的位置沿的传送机构传送染色池的方向前后设置。
在其中一个实施例中，在染色流程开始时先将染液注入染色池，然后再放入需要染色的涂片，这样就完全避免了溶血的风险，保证了染色的质量。
上述推片染色机的染色方法结合上述推片染色机的结构及工作过程较容易理解，不再详述。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对本发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。

Claims (11)

  1. 一种推片染色机染色装置，其特征在于，包括：
    染色池(20)；
    传送机构(100)，用于在封闭传送通道上循环传送所述染色池；
    作业位(600，600a，600b，600c，600d)，设置在所述封闭传送通道上，所述作业位包括：
    阻挡与分离机构(30)，用于将待在本作业位处理的染色池阻挡并将处理后的染色池分离使分离后的所述染色池在所述传送机构的传送下沿所述封闭传送通道传送至下一个作业位；
    试剂给排机构(40)，用于向染色池内注入试剂和/或排出染色池内的试剂；
    驱动机构(200)，用于驱动所述阻挡与分离机构。
  2. 根据权利要求1所述的推片染色机染色装置，其特征在于，所述的传送机构包括由电机驱动的圆盘(110)，所述的圆盘上设有一圈用于悬挂染色池的环状开口(112)。
  3. 根据权利要求1所述的推片染色机染色装置，其特征在于，所述染色池包括本体(21)，所述的本体设置有容纳试剂及涂片（60）的开口腔体(22)，本体两侧设有将染色池悬挂于传送机构的悬挂端(23)，所述的悬挂端开设有供阻挡与分离机构分离用的插槽(24)。
  4. 根据权利要求1所述的推片染色机染色装置，其特征在于，所述的阻挡与分离机构包括与所述的驱动机构相连的爪臂(300，300a，300b)，该爪臂在所述的驱动机构的驱动下移动，所述的爪臂包括阻挡部(310，310a，310b)、分离部(320，320a，320b)以及固定连接所述阻挡部和分离部的连接部(330)，所述的阻挡部和分离部在所述的驱动机构的驱动下分别依次位于所述的染色池的封闭传送通道内，且所述的阻挡部和分离部在所述通道内的位置沿所述的传送机构传送染色池的方向前后设置。
  5. 根据权利要求4所述的推片染色机染色装置，其特征在于，所述的爪臂的数量为两个，同一爪臂的阻挡部和分离部设于目标染色池的两侧，两个爪臂（300a，300b）的阻挡部（310a，310b)均设置于目标染色池传送方向的前方，两个爪臂的分离部（320a，320b）均设置于目标染色池传送方向的后方。
  6. 根据权利要求1所述的推片染色机染色装置，其特征在于，所述的每个作业位还包括用于检测染色池位置的检测装置(70)，所述的检测装置与所述驱动机构连接，所述染色池还设置有用于检测的位置检测片(25)。
  7. 一种推片染色机的染色方法，包括如下步骤：
    驱动传送机构移动，在封闭传送通道上循环传送染色池；
    将待在本作业位处理的染色池阻挡；
    对阻挡在本作业位的染色池进行处理；
    将处理后的染色池分离并沿所述封闭传送通道传送至下一作业位。
  8. 根据权利要求7所述的推片染色机的染色方法，其特征在于，所述处理为阻挡预定时间、向染色池内注入试剂和排出染色池内的试剂中的至少一种。
  9. 根据权利要求7所述的推片染色机的染色方法，其特征在于，所述封闭传送通道为圆形通道。
  10. 根据权利要求7所述的推片染色机的染色方法，其特征在于，
    所述将待在本作业位处理的染色池阻挡的步骤为：驱动爪臂的阻挡部将所述阻挡部置于染色池的封闭传送通道内的前方；
    所述将处理后的染色池分离的步骤为：驱动爪臂的阻挡部离开染色池的封闭传送通道的同时驱动爪臂的分离部置于染色池的封闭传送通道内，所述的阻挡部和分离部在所述通道内的位置沿所述的传送机构传送染色池的方向前后设置。
  11. 根据权利要求7至10中任意一项所述的推片染色机的染色方法，其特征在于，还包括先向染色池内注入染液，再放入需要染色的涂片的步骤。
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