WO2013055142A3 - 두 종 입자를 이용한 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체 - Google Patents

두 종 입자를 이용한 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체 Download PDF

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WO2013055142A3
WO2013055142A3 PCT/KR2012/008301 KR2012008301W WO2013055142A3 WO 2013055142 A3 WO2013055142 A3 WO 2013055142A3 KR 2012008301 W KR2012008301 W KR 2012008301W WO 2013055142 A3 WO2013055142 A3 WO 2013055142A3
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bio
conjugate
functional bio
material conjugate
manufacturing
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PCT/KR2012/008301
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French (fr)
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WO2013055142A2 (ko
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김민곤
성윤주
변주영
오영경
정효암
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한국생명공학연구원
광주과학기술원
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Priority to CN201280050559.4A priority Critical patent/CN104011545B/zh
Priority to US14/351,291 priority patent/US9880162B2/en
Publication of WO2013055142A2 publication Critical patent/WO2013055142A2/ko
Publication of WO2013055142A3 publication Critical patent/WO2013055142A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/02—Linear peptides containing at least one abnormal peptide link
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/773—Nanoparticle, i.e. structure having three dimensions of 100 nm or less
    • Y10S977/774—Exhibiting three-dimensional carrier confinement, e.g. quantum dots

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체에 관한 것으로, 더욱 상세하게는 미생물 등을 검출하는 바이오센서로 이용되는 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체에 관한 것이다. 상기 다기능 생체물질 접합체의 제조방법은 (a) 자성 또는 형광특성을 가지는 제 1 나노입자에 단백질을 코팅시키는 단계; (b) 상기 단백질이 코팅된 제 1 나노입자에 금속 특성을 가지는 제2 나노입자를 흡착시켜 접합체를 제조하는 단계; 및 (c) 상기 접합체에 생체물질을 흡착시켜 다기능 생체물질 접합체를 제조하는 단계를 포함한다. 본 발명에 따르면, 본 발명에 따른 다기능 생체물질 접합체의 제조방법은 두 종의 입자를 이용함으로써, 나노입자의 침전을 방지하면서 생체물질을 손쉽게 고정화할 수 있으며, 멀티 기능을 가지는 생체물질 접합체를 제조할 수 있다. 또한, 상기 방법으로 제조된 다기능성 생체물질 접합체는 미생물 검출을 위하여 사용할 수 있으며, 101cfu농도의 미생물까지 검출할 수 있다.
PCT/KR2012/008301 2011-10-14 2012-10-12 두 종 입자를 이용한 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체 WO2013055142A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201280050559.4A CN104011545B (zh) 2011-10-14 2012-10-12 利用两种粒子的多功能生物材料接合体的制备方法及从中制得的多功能生物材料接合体
US14/351,291 US9880162B2 (en) 2011-10-14 2012-10-12 Method for manufacturing multi-functional bio-material conjugate using two kinds of particle, and multi-functional bio-material conjugate manufactured by means of same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110105463 2011-10-14
KR10-2011-0105463 2011-10-14

Publications (2)

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WO2013055142A2 WO2013055142A2 (ko) 2013-04-18
WO2013055142A3 true WO2013055142A3 (ko) 2013-07-04

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PCT/KR2012/008301 WO2013055142A2 (ko) 2011-10-14 2012-10-12 두 종 입자를 이용한 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체

Country Status (4)

Country Link
US (1) US9880162B2 (ko)
KR (1) KR101444424B1 (ko)
CN (1) CN104011545B (ko)
WO (1) WO2013055142A2 (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101427146B1 (ko) * 2013-11-13 2014-08-07 단국대학교 산학협력단 생체 분자 분석 키트 및 이를 이용한 생체 분자 분석 방법
TWI586807B (zh) * 2013-11-21 2017-06-11 中央研究院 偵測檢體內金黃色葡萄球菌的方法
CN104237501B (zh) * 2014-08-13 2016-04-20 南京大学 一种抗体修饰的纳米银及其试剂盒与应用
CN106680345A (zh) * 2016-12-20 2017-05-17 深圳大学 磁性复合纳米材料、电化学生物传感器及制备、构建方法
CN106770135A (zh) * 2017-01-25 2017-05-31 东南大学 金属增强荧光信号的编码微球及其制备方法和应用
US20180284110A1 (en) * 2017-03-30 2018-10-04 Imra America, Inc. Clustered precious metal nanoparticles in a stable colloidal suspension and biological applications using the same
CN108318694A (zh) * 2018-01-29 2018-07-24 厦门大学 一种采用免疫试纸条对抗原进行定量检测的方法
KR102105263B1 (ko) * 2018-08-09 2020-04-27 (주) 비비비 자성 나노입자를 이용한 바이오센서, 이를 이용하는 검출 장치 및 검출 방법
WO2020066654A1 (ja) * 2018-09-28 2020-04-02 富士フイルム株式会社 免疫クロマトグラフィー用複合粒子及びその製造方法、並びに、免疫クロマトグラフィー
JPWO2020203228A1 (ko) * 2019-03-29 2020-10-08
KR102439357B1 (ko) * 2021-02-17 2022-09-02 서울대학교산학협력단 변환기능 링커 기반의 금 나노입자의 응집 시스템을 이용한 현장용 유해물질 검출키트 및 그를 이용한 유해물질의 검출방법
FR3134822A1 (fr) * 2022-04-26 2023-10-27 bioMérieux milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.

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US20030082237A1 (en) * 2001-10-02 2003-05-01 Jennifer Cha Nanoparticle assembled hollow spheres
US20090117002A1 (en) * 2005-05-27 2009-05-07 Ohio University Nanoparticle assemblies with molecular springs
KR20090116142A (ko) * 2008-05-06 2009-11-11 한국식품연구원 식중독균 검출을 위한 자성 코어 금 나노입자 및 그제조방법, 상기 나노입자를 이용한 식중독균 검출방법

Also Published As

Publication number Publication date
KR20130040715A (ko) 2013-04-24
CN104011545B (zh) 2016-02-17
US9880162B2 (en) 2018-01-30
CN104011545A (zh) 2014-08-27
KR101444424B1 (ko) 2014-09-29
US20140272945A1 (en) 2014-09-18
WO2013055142A2 (ko) 2013-04-18

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