WO2013048185A3 - 세포 내에서의 빛을 이용한 가역적 단백질 나노클러스터 형성방법 - Google Patents

세포 내에서의 빛을 이용한 가역적 단백질 나노클러스터 형성방법 Download PDF

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WO2013048185A3
WO2013048185A3 PCT/KR2012/007927 KR2012007927W WO2013048185A3 WO 2013048185 A3 WO2013048185 A3 WO 2013048185A3 KR 2012007927 W KR2012007927 W KR 2012007927W WO 2013048185 A3 WO2013048185 A3 WO 2013048185A3
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protein
light
forming
expression vector
heterodimer
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PCT/KR2012/007927
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English (en)
French (fr)
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WO2013048185A2 (ko
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허원도
이상규
박혜림
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한국과학기술원
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Priority claimed from KR1020110100334A external-priority patent/KR101354601B1/ko
Priority claimed from KR1020110130081A external-priority patent/KR101355970B1/ko
Application filed by 한국과학기술원 filed Critical 한국과학기술원
Priority to US14/348,757 priority Critical patent/US9708381B2/en
Publication of WO2013048185A2 publication Critical patent/WO2013048185A2/ko
Publication of WO2013048185A3 publication Critical patent/WO2013048185A3/ko
Priority to US15/618,770 priority patent/US10604553B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11017—Ca2+/Calmodulin-dependent protein kinase (2.7.11.17)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 단백질간의 상호작용 및 기능을 효율적으로 분석하기 위하여, 광유도 이형 이합체 형성 단백질 및 제1자기조립단백질을 포함하는 제1융합단백질을 암호화하는 폴리뉴클레오티드를 포함하는 제1발현벡터; 및 상기 광유도 동형 이합체 형성 단백질과 동형 이합체를 형성하는 짝 단백질 또는 상기 짝 단백질 및 제2자기조립단백질을 포함하는 제2융합단백질을 암호화하는 폴리뉴클레오티드를 포함하는 제2발현벡터를 준비하는 발현벡터 준비단계; 상기 제1발현벡터 및 제2발현벡터로 세포, 조직 또는 개체를 형질전환시키는 형질전환 세포, 조직 또는 개체의 제조단계; 및 상기 형질전환 세포, 조직 또는 개체에 상기 광유도 이형 이합체 단백질과 상기 짝 단백질 사이의 이형 이합체 형성을 유도하는 파장의 빛을 조사하는 광조사단계를 포함하는 광유도 단백질 나노클러스터 형성방법을 제공한다.
PCT/KR2012/007927 2011-09-30 2012-09-28 세포 내에서의 빛을 이용한 가역적 단백질 나노클러스터 형성방법 WO2013048185A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/348,757 US9708381B2 (en) 2011-09-30 2012-09-28 Method for forming a reversible protein nanocluster using light in a cell
US15/618,770 US10604553B2 (en) 2011-09-30 2017-06-09 Method for forming a reversible protein nanocluster using light in a cell

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2011-0100334 2011-09-30
KR1020110100334A KR101354601B1 (ko) 2011-09-30 2011-09-30 광유도 나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트
KR10-2011-0130081 2011-12-07
KR1020110130081A KR101355970B1 (ko) 2011-12-07 2011-12-07 광유도 나노클러스터 형성을 이용한 단백질 기능의 가역적 저해방법 및 키트

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/348,757 A-371-Of-International US9708381B2 (en) 2011-09-30 2012-09-28 Method for forming a reversible protein nanocluster using light in a cell
US15/618,770 Continuation US10604553B2 (en) 2011-09-30 2017-06-09 Method for forming a reversible protein nanocluster using light in a cell

Publications (2)

Publication Number Publication Date
WO2013048185A2 WO2013048185A2 (ko) 2013-04-04
WO2013048185A3 true WO2013048185A3 (ko) 2013-05-23

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US (2) US9708381B2 (ko)
WO (1) WO2013048185A2 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555748B (zh) * 2013-10-30 2016-03-30 贵州省烟草科学研究院 反向调节烟草NtPSY基因功能的载体组合物
CN106164344B (zh) * 2014-02-07 2018-12-18 韩国生命工学研究院 使用流式细胞仪测定蛋白纳米颗粒分子间相互作用的方法
US10702581B2 (en) 2015-05-04 2020-07-07 Ilias Biologics Inc. Compositions containing protein loaded exosome and methods for preparing and delivering the same
WO2017053629A2 (en) * 2015-09-22 2017-03-30 William Marsh Rice University Light-controlled gene delivery with virus vectors through incorporation of optogenetic proteins and genetic insertion of non-conformationally constrained peptides
GB201518477D0 (en) * 2015-10-19 2015-12-02 Isis Innovation Biomolecule separation and modification
IL259023B (en) * 2016-09-30 2022-09-01 Cellex Life Sciences Incorporated Preparations containing protein-charged exosomes, methods for their production and uses thereof
US10526380B2 (en) * 2017-10-26 2020-01-07 St. Jude Children's Research Hospital Fusion protein and nucleic acid molecule for light-dependent stress granule assembly
JP2023550143A (ja) * 2020-11-20 2023-11-30 アルベルト－ルートヴィヒ－ウニヴェルジテート フライブルク 光制御ウイルス導入
CN113265427B (zh) * 2021-06-02 2022-09-09 呈诺再生医学科技(珠海横琴新区)有限公司 一种iPSC分化动态监控系统

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Publication number Publication date
WO2013048185A2 (ko) 2013-04-04
US20150284439A1 (en) 2015-10-08
US9708381B2 (en) 2017-07-18
US20170275346A1 (en) 2017-09-28
US10604553B2 (en) 2020-03-31

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