WO2013042917A3 - 중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법 - Google Patents

중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법 Download PDF

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Publication number
WO2013042917A3
WO2013042917A3 PCT/KR2012/007451 KR2012007451W WO2013042917A3 WO 2013042917 A3 WO2013042917 A3 WO 2013042917A3 KR 2012007451 W KR2012007451 W KR 2012007451W WO 2013042917 A3 WO2013042917 A3 WO 2013042917A3
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WO
WIPO (PCT)
Prior art keywords
enzyme treatment
proteins
treatment apparatus
hollow fiber
fiber membrane
Prior art date
Application number
PCT/KR2012/007451
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English (en)
French (fr)
Other versions
WO2013042917A2 (ko
Inventor
강덕진
박상열
김숙경
Original Assignee
한국표준과학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국표준과학연구원 filed Critical 한국표준과학연구원
Priority to US14/345,835 priority Critical patent/US9598717B2/en
Priority to EP12833157.6A priority patent/EP2759591A4/en
Publication of WO2013042917A2 publication Critical patent/WO2013042917A2/ko
Publication of WO2013042917A3 publication Critical patent/WO2013042917A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C12M25/12—Hollow fibers or tubes the culture medium flowing outside the fiber or tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법에 관한 것으로, 상기 본 발명에 따른 단백질의 효소 처리 장치는 기존 효소처리과정에서 발생되는 효소활성도의 낮은 재현성을 근본적으로 해결하여 효소처리과정을 통해 회수되는 펩티드의 회수율이 높을 뿐 아니라 단일 단계로 분리ㆍ정제함으로써 정제에 필요한 시간을 줄이고 더 높은 수율을 가져올 수 있으며, 특히 인간의 질병과 관련된 바이오마커 발굴 연구에 있어서는 단백질의 검출 감도를 고효율로 하면서도 통계적인 분석방법을 통해 질병 특이적 단백질 바이오 마커 개발에 용이하게 적용할 수 있다.
PCT/KR2012/007451 2011-09-19 2012-09-18 중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법 WO2013042917A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/345,835 US9598717B2 (en) 2011-09-19 2012-09-18 Enzyme treatment apparatus for proteins using a hollow fiber membrane, and on-line proteomics method using same
EP12833157.6A EP2759591A4 (en) 2011-09-19 2012-09-18 ENZYME TREATMENT DEVICE FOR PROTEINS WITH A HOLLOW FIBER MEMBRANE AND ONLINE PROTEOMIC PROCESS THEREWITH

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0093950 2011-09-19
KR1020110093950A KR101298527B1 (ko) 2011-09-19 2011-09-19 중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법

Publications (2)

Publication Number Publication Date
WO2013042917A2 WO2013042917A2 (ko) 2013-03-28
WO2013042917A3 true WO2013042917A3 (ko) 2013-05-23

Family

ID=47914999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/007451 WO2013042917A2 (ko) 2011-09-19 2012-09-18 중공사막을 이용한 단백질의 효소 처리장치 및 이를 이용한 온-라인 프로테오믹스 방법

Country Status (4)

Country Link
US (1) US9598717B2 (ko)
EP (1) EP2759591A4 (ko)
KR (1) KR101298527B1 (ko)
WO (1) WO2013042917A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101675303B1 (ko) 2014-08-19 2016-11-11 한국표준과학연구원 마이크로 중공사막 효소 반응기-텐덤 질량분석법을 이용한 단세포군 항체 기반 온라인 인단백질 프로테오믹스 분석방법
GB201706799D0 (en) * 2017-04-28 2017-06-14 Univ Oslo Enzyme reactor vessel

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070001927A (ko) * 2004-01-21 2007-01-04 도레이 가부시끼가이샤 분획 장치 및 분획 방법
JP2007044043A (ja) * 2005-08-10 2007-02-22 Agilent Technol Inc 分離検出法におけるオンライン酵素消化
KR100792683B1 (ko) * 2006-05-09 2008-01-09 연세대학교 산학협력단 모세관등전집중-중공사흐름장흐름분획법을 이용한 단백질분리장치 및 분리방법

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4315819A (en) * 1978-06-12 1982-02-16 Monsanto Company Hollow fiber permeator apparatus
US4443540A (en) * 1980-05-09 1984-04-17 University Of Illinois Foundation Protein hydrolysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070001927A (ko) * 2004-01-21 2007-01-04 도레이 가부시끼가이샤 분획 장치 및 분획 방법
JP2007044043A (ja) * 2005-08-10 2007-02-22 Agilent Technol Inc 分離検出法におけるオンライン酵素消化
KR100792683B1 (ko) * 2006-05-09 2008-01-09 연세대학교 산학협력단 모세관등전집중-중공사흐름장흐름분획법을 이용한 단백질분리장치 및 분리방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2759591A4 *

Also Published As

Publication number Publication date
KR20130030464A (ko) 2013-03-27
EP2759591A2 (en) 2014-07-30
KR101298527B1 (ko) 2013-08-22
WO2013042917A2 (ko) 2013-03-28
EP2759591A4 (en) 2015-04-29
US20140302545A1 (en) 2014-10-09
US9598717B2 (en) 2017-03-21

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