WO2007117444A3 - Protein detection by aptamers - Google Patents

Protein detection by aptamers Download PDF

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Publication number
WO2007117444A3
WO2007117444A3 PCT/US2007/008274 US2007008274W WO2007117444A3 WO 2007117444 A3 WO2007117444 A3 WO 2007117444A3 US 2007008274 W US2007008274 W US 2007008274W WO 2007117444 A3 WO2007117444 A3 WO 2007117444A3
Authority
WO
WIPO (PCT)
Prior art keywords
aptamers
methods
detecting
protein detection
measuring proteins
Prior art date
Application number
PCT/US2007/008274
Other languages
French (fr)
Other versions
WO2007117444A2 (en
Inventor
Yinghe Hu
Wenze Niu
Nan Jiang
Original Assignee
Yinghe Hu
Wenze Niu
Nan Jiang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN 200610025342 external-priority patent/CN1912138A/en
Priority claimed from CNB2006100259115A external-priority patent/CN100500865C/en
Application filed by Yinghe Hu, Wenze Niu, Nan Jiang filed Critical Yinghe Hu
Publication of WO2007117444A2 publication Critical patent/WO2007117444A2/en
Publication of WO2007117444A3 publication Critical patent/WO2007117444A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Abstract

The invention provides a system for performing large scale proteomic expression studies. The methods of the invention are based on detecting and/or measuring proteins using a plurality of aptamers that recognize oligopeptide epitopes on a target protein. Further, the present invention relates to methods for diagnosing and staging diseases by detecting and/or measuring proteins associated with certain clinical conditions. Methods of selecting aptamers, diagnostic kits are also encompassed.
PCT/US2007/008274 2006-03-31 2007-03-30 Protein detection by aptamers WO2007117444A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN 200610025342 CN1912138A (en) 2006-03-31 2006-03-31 Oligonucleotide adapter of identification of protein squence applied in protein tissue research
CN200610025342.4 2006-03-31
CN200610025911.5 2006-04-21
CNB2006100259115A CN100500865C (en) 2006-04-21 2006-04-21 Method for detecting CatD protein by identifying a plurality of epitopes synchronously

Publications (2)

Publication Number Publication Date
WO2007117444A2 WO2007117444A2 (en) 2007-10-18
WO2007117444A3 true WO2007117444A3 (en) 2008-01-24

Family

ID=38480609

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/008274 WO2007117444A2 (en) 2006-03-31 2007-03-30 Protein detection by aptamers

Country Status (1)

Country Link
WO (1) WO2007117444A2 (en)

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CN102093469B (en) * 2009-12-11 2014-07-30 光明乳业股份有限公司 Milk-derived antioxidant active peptide and application thereof
WO2012004790A2 (en) 2010-07-06 2012-01-12 Aptateck Bio Ltd. Nucleic acid aptamer-based diagnostic methods with novel techniques for signal enhancement
GB201018125D0 (en) 2010-10-26 2010-12-08 Marealis As Peptide
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
CN103428730B (en) * 2012-05-18 2019-03-26 中兴通讯股份有限公司 A kind of minimum drive test qos measurement result transmission method and device
US9765375B2 (en) * 2013-06-28 2017-09-19 General Electric Company Methods for developing binding-elements and uses thereof
US9828599B2 (en) * 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
KR101572606B1 (en) * 2014-11-24 2015-11-27 강원대학교산학협력단 Peptides derived from Sfrp5 and cosmetic compositions for skin-whitening comprising the same
CN110028549B (en) * 2016-07-07 2022-08-05 华东理工大学 Antihypertensive peptide and antihypertensive protein and application thereof
CN110945143A (en) * 2016-11-02 2020-03-31 拜欧亿思有限公司 Clustering quantification method of target protein by next generation sequencing method and application thereof
EP3548652B1 (en) 2016-12-01 2024-04-03 Nautilus Subsidiary, Inc. Methods of assaying proteins
RU2766160C2 (en) * 2017-05-17 2022-02-08 Юю Фарма, Инк. New peptide and pharmaceutical composition for treatment of eye disease, containing peptide as active pharmaceutical ingredient
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US11530276B2 (en) * 2017-10-25 2022-12-20 4TEEN4 Pharmaceuticals GmbH DPP3 binder directed to and binding to specific DPP3-epitopes and its use in the prevention or treatment of diseases / acute conditions that are associated with oxidative stress
WO2019090350A2 (en) * 2017-11-06 2019-05-09 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Wet adhesive peptide materials and their use
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WO2019134526A1 (en) * 2018-01-03 2019-07-11 西交利物浦大学 Membrane-type metalloprotease inhibitory protein and pharmaceutical and pharmaceutical composition containing same, and respective uses thereof
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JP2021532350A (en) * 2018-07-31 2021-11-25 ザ・リージエンツ・オブ・ザ・ユニバーシテイ・オブ・コロラド、ア・ボデイー・コーポレイト Systems and methods for applying machine learning to analyze microcopy images in high-throughput systems
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CN113340864B (en) * 2021-06-07 2023-04-07 郑州轻工业大学 Aptamer sensor for secondary amplification of INS signal based on MEF effect and preparation method and application thereof
WO2023277514A1 (en) * 2021-06-28 2023-01-05 고려대학교 산학협력단 Peptide having anticancer activity, and use thereof
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CN116554264B (en) * 2021-09-30 2023-11-28 中国农业大学 Oligopeptide separated from Rosa roxburghii, preparation method, oxidation resistance application and product thereof
CN114199848B (en) * 2021-12-17 2023-01-17 无锡佰翱得生物科学有限公司 High-throughput protein expression detection method based on protein ligase
CN114624442B (en) * 2022-05-16 2022-08-12 南京颐兰贝生物科技有限责任公司 Chemiluminescence carcinoembryonic antigen detection reagent based on aptamer signal amplification and preparation method thereof
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