WO2013037933A3 - Enzymes - Google Patents

Enzymes Download PDF

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Publication number
WO2013037933A3
WO2013037933A3 PCT/EP2012/068041 EP2012068041W WO2013037933A3 WO 2013037933 A3 WO2013037933 A3 WO 2013037933A3 EP 2012068041 W EP2012068041 W EP 2012068041W WO 2013037933 A3 WO2013037933 A3 WO 2013037933A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
endo
beta
activity
compositions
Prior art date
Application number
PCT/EP2012/068041
Other languages
English (en)
Other versions
WO2013037933A2 (fr
Inventor
Jens Frisbæk SØRENSEN
Lone Brønd MILLER
Original Assignee
Dupont Nutrition Biosciences Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to PL12759133T priority Critical patent/PL2756078T3/pl
Priority to BR112014005873-3A priority patent/BR112014005873B1/pt
Priority to MX2014002950A priority patent/MX353745B/es
Priority to CN201280044661.3A priority patent/CN103814129A/zh
Application filed by Dupont Nutrition Biosciences Aps filed Critical Dupont Nutrition Biosciences Aps
Priority to JP2014530234A priority patent/JP6084617B2/ja
Priority to ES12759133.7T priority patent/ES2645921T3/es
Priority to AU2012307299A priority patent/AU2012307299C1/en
Priority to DK12759133.7T priority patent/DK2756078T3/da
Priority to EA201490624A priority patent/EA027084B1/ru
Priority to EP12759133.7A priority patent/EP2756078B9/fr
Priority to US14/344,508 priority patent/US9683224B2/en
Publication of WO2013037933A2 publication Critical patent/WO2013037933A2/fr
Publication of WO2013037933A3 publication Critical patent/WO2013037933A3/fr
Priority to ZA2014/00889A priority patent/ZA201400889B/en
Priority to US15/590,867 priority patent/US20170314004A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/14—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C12C11/003—Fermentation of beerwort
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La présente invention concerne de nouvelles enzymes ayant des propriétés améliorées et des compositions comprenant ces enzymes appropriées pour l'utilisation dans la production d'un aliment, d'un aliment pour animaux ou d'un produit de boisson malté, tel que dans un procédé de brassage.
PCT/EP2012/068041 2011-09-14 2012-09-14 Enzymes WO2013037933A2 (fr)

Priority Applications (13)

Application Number Priority Date Filing Date Title
ES12759133.7T ES2645921T3 (es) 2011-09-14 2012-09-14 Composiciones que comprenden enzimas con actividad de endo-1,4-beta-xilanasa y enzimas con actividad de endo-1,3(4)-beta-glucanasa
MX2014002950A MX353745B (es) 2011-09-14 2012-09-14 Composiciones que comprenden enzimas con activiad de endo-1,4-beta-xilanasa y enzimas con actividad de endo-1,3(4)-beta glucanasa.
CN201280044661.3A CN103814129A (zh) 2011-09-14 2012-09-14 包含具有内切–1,4–β–木聚糖酶活性的酶和具有内切–1,3(4)–β葡聚糖酶活性的酶的组合物
DK12759133.7T DK2756078T3 (da) 2011-09-14 2012-09-14 Sammensætninger, der omfatter enzymer med endo-1, 4-beta- xylanaseaktivitet og enzymer med endo-1,3(4)-betaglucanaseaktivitet
JP2014530234A JP6084617B2 (ja) 2011-09-14 2012-09-14 酵素
BR112014005873-3A BR112014005873B1 (pt) 2011-09-14 2012-09-14 Composição, uso de uma composição, método de alteração da filtrabilidade, método de redução da pressão acumulada, método para a produção de um alimento, método para a produção de uma pasta de brassagem e método para a produção de um biocombustível
AU2012307299A AU2012307299C1 (en) 2011-09-14 2012-09-14 Compositions comprising enzymes with endo - 1, 4 - beta - xylanase activity and enzymes with endo - 1, 3 (4) - beta glucanase activity
PL12759133T PL2756078T3 (pl) 2011-09-14 2012-09-14 Kompozycje zawierające enzymy o aktywności endo-1,4-beta-ksylanazy i enzymy o aktywności endo-1, 3(4)-beta glukanazy
EA201490624A EA027084B1 (ru) 2011-09-14 2012-09-14 Ферменты
EP12759133.7A EP2756078B9 (fr) 2011-09-14 2012-09-14 Compositions comprenant des enzymes avec l'activité endo-1, 4-beta-xylanase et des enzymes avec l'activité endo-1,3(4)-beta glucanase
US14/344,508 US9683224B2 (en) 2011-09-14 2012-09-14 Enzymes
ZA2014/00889A ZA201400889B (en) 2011-09-14 2014-02-05 Compositions comprising enzymes with endo - 1,4 - beta -xylanase activity and enzymes with endo-1,3 (4)-beta glucanase activity
US15/590,867 US20170314004A1 (en) 2011-09-14 2017-05-09 Enzymes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201161534574P 2011-09-14 2011-09-14
US61/534,574 2011-09-14
EP11181241.8 2011-09-14
EP11181241 2011-09-14
US201261676535P 2012-07-27 2012-07-27
US61/676,535 2012-07-27

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/344,508 A-371-Of-International US9683224B2 (en) 2011-09-14 2012-09-14 Enzymes
US15/590,867 Division US20170314004A1 (en) 2011-09-14 2017-05-09 Enzymes

Publications (2)

Publication Number Publication Date
WO2013037933A2 WO2013037933A2 (fr) 2013-03-21
WO2013037933A3 true WO2013037933A3 (fr) 2013-05-30

Family

ID=47883847

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/068041 WO2013037933A2 (fr) 2011-09-14 2012-09-14 Enzymes

Country Status (15)

Country Link
US (2) US9683224B2 (fr)
EP (2) EP3061816A1 (fr)
JP (1) JP6084617B2 (fr)
CN (2) CN103814129A (fr)
AR (1) AR087885A1 (fr)
AU (1) AU2012307299C1 (fr)
BR (1) BR112014005873B1 (fr)
DK (1) DK2756078T3 (fr)
EA (2) EA027084B1 (fr)
ES (1) ES2645921T3 (fr)
HU (1) HUE037237T2 (fr)
MX (1) MX353745B (fr)
PL (1) PL2756078T3 (fr)
WO (1) WO2013037933A2 (fr)
ZA (1) ZA201400889B (fr)

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Publication number Priority date Publication date Assignee Title
AU2012387042A1 (en) * 2012-08-03 2015-02-19 Dupont Nutrition Biosciences Aps Enzymes
US9556465B2 (en) 2013-05-10 2017-01-31 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP3027743A1 (fr) 2013-07-29 2016-06-08 Danisco US Inc. Variants enzymatiques
EP3017706A1 (fr) 2014-11-05 2016-05-11 Dupont Nutrition Biosciences ApS Enzymes de maltage
CN106906198B (zh) * 2015-12-23 2019-12-13 东莞泛亚太生物科技有限公司 提升耐温性的纤维素酶
BR112018016297A2 (pt) * 2016-02-18 2019-01-22 Biopract Gmbh arabinanase e usos da mesma
JP7103948B2 (ja) 2016-03-04 2022-07-20 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
US20180072972A1 (en) * 2016-09-09 2018-03-15 Alpha Revolution, Inc. Systems, devices and methods for fermenting beverages
EP3703661A1 (fr) 2017-11-02 2020-09-09 Danisco US Inc. Compositions de matrices solides à point de congélation abaissé pour la granulation à l'état fondu d'enzymes
CN110714037A (zh) * 2019-11-29 2020-01-21 江南大学 一种木聚糖酶的制备方法及其在啤酒生产中的应用
CN116286751B (zh) * 2023-05-15 2023-07-25 北京市科学技术研究院 催化效率提高的双功能纤维素酶突变体及其应用

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