WO2013012293A3 - 글리세롤 발효 미생물 변이체를 이용하여 디올화합물을 고수율로 생산하는 방법 - Google Patents

글리세롤 발효 미생물 변이체를 이용하여 디올화합물을 고수율로 생산하는 방법 Download PDF

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WO2013012293A3
WO2013012293A3 PCT/KR2012/005832 KR2012005832W WO2013012293A3 WO 2013012293 A3 WO2013012293 A3 WO 2013012293A3 KR 2012005832 W KR2012005832 W KR 2012005832W WO 2013012293 A3 WO2013012293 A3 WO 2013012293A3
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Prior art keywords
high yield
diol compound
variant
glycerol
fermentation microorganism
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PCT/KR2012/005832
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English (en)
French (fr)
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WO2013012293A9 (ko
WO2013012293A2 (ko
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김철호
서정우
오백록
허선연
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한국생명공학연구원
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Publication of WO2013012293A2 publication Critical patent/WO2013012293A2/ko
Publication of WO2013012293A3 publication Critical patent/WO2013012293A3/ko
Publication of WO2013012293A9 publication Critical patent/WO2013012293A9/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 변이균주를 이용하여, 글리세롤로부터 디올화합물을 제조하는 방법에 관한 것으로, 글리세롤 발효능력을 보유한 미생물에서 락테이트 디하이드로게나아제를 코딩하는 유전자를 결손시킨 변이체를 이용하여 고수율로 1,3-프로판디올 및 2,3-부탄디올을 생산하는 방법에 관한 것이다. 본 발명에 따르면, 글리세롤 발효 미생물 변이체의 새로운 배양 방법을 통하여 1,3-프로판디올 및 2,3-부탄디올의 생산량을 월등히 개선할 수 있다.
PCT/KR2012/005832 2011-07-21 2012-07-20 글리세롤 발효 미생물 변이체를 이용하여 디올화합물을 고수율로 생산하는 방법 WO2013012293A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110072431A KR20130011343A (ko) 2011-07-21 2011-07-21 글리세롤 발효 미생물 변이체를 이용하여 디올화합물을 고수율로 생산하는 방법
KR10-2011-0072431 2011-07-21

Publications (3)

Publication Number Publication Date
WO2013012293A2 WO2013012293A2 (ko) 2013-01-24
WO2013012293A3 true WO2013012293A3 (ko) 2013-03-28
WO2013012293A9 WO2013012293A9 (ko) 2013-05-16

Family

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Application Number Title Priority Date Filing Date
PCT/KR2012/005832 WO2013012293A2 (ko) 2011-07-21 2012-07-20 글리세롤 발효 미생물 변이체를 이용하여 디올화합물을 고수율로 생산하는 방법

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KR (1) KR20130011343A (ko)
WO (1) WO2013012293A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101876980B1 (ko) 2016-11-29 2018-07-11 한국생명공학연구원 글리세롤로부터 1,3-프로판디올을 고생산하는 락토바실러스 루테리 ch53 균주 및 이의 용도
KR102401557B1 (ko) * 2020-07-31 2022-05-26 경희대학교 산학협력단 글리세롤을 동화하는 메탄자화균의 개발 및 이를 이용한 고부가가치 알코올 생산방법
KR102558672B1 (ko) * 2022-05-17 2023-07-25 주식회사 엑티브온 1,3-프로판디올 생산 변이 미생물 및 이를 이용한 1,3-프로판디올의 생산 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100137655A1 (en) * 2006-10-31 2010-06-03 Metabolic Explorer Process for the biological production of 1,3-propanediol from glycerol with high yield
KR20100102928A (ko) * 2009-03-12 2010-09-27 한국생명공학연구원 글리세롤 산화대사경로를 차단시킨 재조합 균주를 이용한 １、３―프로판디올의 제조방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100137655A1 (en) * 2006-10-31 2010-06-03 Metabolic Explorer Process for the biological production of 1,3-propanediol from glycerol with high yield
KR20100102928A (ko) * 2009-03-12 2010-09-27 한국생명공학연구원 글리세롤 산화대사경로를 차단시킨 재조합 균주를 이용한 １、３―프로판디올의 제조방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
XU, Y. ET AL.: "Metabolism in 1,3-Propanediol Fed-Batch Fermentation by a D-Lactate Deficient Mutant of Klebsiella pneumoniae", BIOTECHNOLOGY & BIOENGINEERING, vol. 104, no. 5, 2009, pages 965 - 972 *
YANG, G. ET AL.: "Fermentation of 1,3-propanediol by a lactate deficient mutant of Klebsiella oxytoca under microaerobic conditions", APPL MICROBIOL BIOTECHNOL, vol. 73, 2007, pages 1017 - 1024 *

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Publication number Publication date
KR20130011343A (ko) 2013-01-30
WO2013012293A9 (ko) 2013-05-16
WO2013012293A2 (ko) 2013-01-24

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