WO2013008033A1 - Améliorations concernant la microscopie à fluorescence - Google Patents

Améliorations concernant la microscopie à fluorescence Download PDF

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Publication number
WO2013008033A1
WO2013008033A1 PCT/GB2012/051680 GB2012051680W WO2013008033A1 WO 2013008033 A1 WO2013008033 A1 WO 2013008033A1 GB 2012051680 W GB2012051680 W GB 2012051680W WO 2013008033 A1 WO2013008033 A1 WO 2013008033A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence
fluorescence microscopy
depletion
light source
emission
Prior art date
Application number
PCT/GB2012/051680
Other languages
English (en)
Inventor
Angus John BAIN
Richard John MARSH
Original Assignee
Ucl Business Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucl Business Plc filed Critical Ucl Business Plc
Publication of WO2013008033A1 publication Critical patent/WO2013008033A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un procédé de génération d'images fluorescentes à résolution sous-longueur d'onde d'un échantillon par une combinaison linéaire d'images fluorescentes enregistrées dans une série de fenêtres temporelles à la suite d'une excitation pulsée de marqueurs fluorescents dans l'échantillon en présence d'une décroissance d'émission stimulée induite par une source lumineuse à décroissance continue d'ondes, ladite décroissance d'émission stimulée se traduisant par une variation spatiale de la durée de vie observée de fluorescence et une évolution de la fonction effective d'étalement de point (PSF) du microscope avec le temps.
PCT/GB2012/051680 2011-07-13 2012-07-13 Améliorations concernant la microscopie à fluorescence WO2013008033A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201111976A GB201111976D0 (en) 2011-07-13 2011-07-13 Super resolution fluorescence microscopy
GB1111976.5 2011-07-13

Publications (1)

Publication Number Publication Date
WO2013008033A1 true WO2013008033A1 (fr) 2013-01-17

Family

ID=44586476

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/051680 WO2013008033A1 (fr) 2011-07-13 2012-07-13 Améliorations concernant la microscopie à fluorescence

Country Status (2)

Country Link
GB (1) GB201111976D0 (fr)
WO (1) WO2013008033A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103279926A (zh) * 2013-05-15 2013-09-04 中国航空工业集团公司沈阳空气动力研究所 一种tsp/psp旋转部件测量的模糊修正方法
WO2014169197A1 (fr) * 2013-04-12 2014-10-16 Duky University Systèmes et procédés pour microscopie avec contraste de phase à super-résolution et éclairage structuré
WO2015055900A2 (fr) 2013-10-14 2015-04-23 Bioaxial Sas Procédé et dispositif de mesure optique
WO2016092161A1 (fr) 2014-12-09 2016-06-16 Bioaxial Sas Procédé et dispositif de mesure optique
US10238279B2 (en) 2015-02-06 2019-03-26 Duke University Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope
US10694939B2 (en) 2016-04-29 2020-06-30 Duke University Whole eye optical coherence tomography(OCT) imaging systems and related methods
US10835119B2 (en) 2015-02-05 2020-11-17 Duke University Compact telescope configurations for light scanning systems and methods of using the same
CN112162079A (zh) * 2020-09-09 2021-01-01 中国科学院过程工程研究所 一种无人值守式熔体热物性参数的测试系统装置及测试方法

Citations (4)

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US3013467A (en) 1957-11-07 1961-12-19 Minsky Marvin Microscopy apparatus
US5731588A (en) 1994-02-01 1998-03-24 Hell; Stefan Process and device for optically measuring a point on a sample with high local resolution
US20100176307A1 (en) 2007-08-18 2010-07-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. STED-Fluorescent Light Microscopy with Two-Photon Excitation
WO2012069076A1 (fr) * 2010-11-22 2012-05-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Microscopie sted avec excitation pulsée, stimulation continue et alignement déclenché d'une lumière de fluorescence émise spontanément

Patent Citations (4)

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US3013467A (en) 1957-11-07 1961-12-19 Minsky Marvin Microscopy apparatus
US5731588A (en) 1994-02-01 1998-03-24 Hell; Stefan Process and device for optically measuring a point on a sample with high local resolution
US20100176307A1 (en) 2007-08-18 2010-07-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. STED-Fluorescent Light Microscopy with Two-Photon Excitation
WO2012069076A1 (fr) * 2010-11-22 2012-05-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Microscopie sted avec excitation pulsée, stimulation continue et alignement déclenché d'une lumière de fluorescence émise spontanément

Non-Patent Citations (12)

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J R LAKOWICZ; A BALTER: "Analysis of excited state processes by phase modulation fluorescence spectroscopy", BIOPHYS. CHEM., vol. 16, 1982, pages 99 - 115
JEFFREY R. MOFFITT ET AL: "Time-gating improves the spatial resolution of STED microscopy", OPTICS EXPRESS, vol. 19, no. 5, 28 February 2011 (2011-02-28), pages 4242 - 4254, XP055002831, ISSN: 1094-4087, DOI: 10.1364/OE.19.004242 *
M G L GUSTAFSSON: "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy", JOURNAL OF MICROSCOPY, vol. 198, no. 2, 2000, pages 82 - 87, XP008083176, DOI: doi:10.1046/j.1365-2818.2000.00710.x
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M. O. LENZ ET AL: "A STED-FLIM microscope applied to imaging the natural killer cell immune synapse", PROCEEDINGS OF SPIE, vol. 7903, 28 February 2011 (2011-02-28), pages 79032D-1 - 79032D-6, XP055041081, ISSN: 0277-786X, DOI: 10.1117/12.875018 *
R M CLEGG; P C SCHNEIDER: "Fluorescence Microscopy & Fluorescent Probes", 1996, PLENUM PRESS, article "Fluorescence lifetime- resolved imaging microscopy"
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014169197A1 (fr) * 2013-04-12 2014-10-16 Duky University Systèmes et procédés pour microscopie avec contraste de phase à super-résolution et éclairage structuré
US9864183B2 (en) 2013-04-12 2018-01-09 Duke University Systems and methods for structured illumination super-resolution phase microscopy
CN103279926A (zh) * 2013-05-15 2013-09-04 中国航空工业集团公司沈阳空气动力研究所 一种tsp/psp旋转部件测量的模糊修正方法
WO2015055900A2 (fr) 2013-10-14 2015-04-23 Bioaxial Sas Procédé et dispositif de mesure optique
EP3926387A1 (fr) 2013-10-14 2021-12-22 Bioaxial SAS Système de super résolution d'images de fluorescence basé sur la diffraction conique incluant un algorithme de reconstruction bayésien
WO2016092161A1 (fr) 2014-12-09 2016-06-16 Bioaxial Sas Procédé et dispositif de mesure optique
US10835119B2 (en) 2015-02-05 2020-11-17 Duke University Compact telescope configurations for light scanning systems and methods of using the same
US10238279B2 (en) 2015-02-06 2019-03-26 Duke University Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope
US10694939B2 (en) 2016-04-29 2020-06-30 Duke University Whole eye optical coherence tomography(OCT) imaging systems and related methods
CN112162079A (zh) * 2020-09-09 2021-01-01 中国科学院过程工程研究所 一种无人值守式熔体热物性参数的测试系统装置及测试方法

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