WO2013002455A1 - Marqueur standard pour distinguer le pays d'origine ou l'âge de ginseng traité, son procédé d'établissement, ou procédé pour distinguer le pays d'origine ou l'âge de ginseng l'utilisant - Google Patents

Marqueur standard pour distinguer le pays d'origine ou l'âge de ginseng traité, son procédé d'établissement, ou procédé pour distinguer le pays d'origine ou l'âge de ginseng l'utilisant Download PDF

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Publication number
WO2013002455A1
WO2013002455A1 PCT/KR2011/007368 KR2011007368W WO2013002455A1 WO 2013002455 A1 WO2013002455 A1 WO 2013002455A1 KR 2011007368 W KR2011007368 W KR 2011007368W WO 2013002455 A1 WO2013002455 A1 WO 2013002455A1
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Prior art keywords
marker
ginseng
determining
formula
age
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PCT/KR2011/007368
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English (en)
Korean (ko)
Inventor
오세량
이형규
송혁환
김두영
김정한
김정희
안경섭
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한국생명공학연구원
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Publication of WO2013002455A1 publication Critical patent/WO2013002455A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5097—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
    • C07J17/005—Glycosides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the present invention relates to a standard marker for determining the origin or age of processed ginseng, a method for establishing the same or a method for determining the origin or age using the same.
  • Ginseng panax ginseng CA Meyer
  • ginseng has been used as a medicinal plant for thousands of years, and ginseng has long evolved to suit its environment. Little by little, he has been able to live well in his environment and has changed himself according to the environment, and ginseng is divided into fresh ginseng, white ginseng, and heungsam dong according to the processing method, and the ginseng of mined natural state is ginseng. Peeled ginseng and dried in sunlight or hot air is called white ginseng. Also, white ginseng is steamed without peeling. Steamed and dried is called red ginseng.
  • Korean ginseng has been known to be the best in Korea.
  • international markets have been encroached by cheap Chinese and American and Canadian ginseng, and Chinese ginseng has recently entered the domestic market through wheat trade, making it difficult to position domestically grown ginseng.
  • Study on the Establishment and Efficacy of Classification System of Wild Ginseng and Chinese Camphor Ginseng "Research Project Report, 2007)
  • overseas ginseng does not have much meaning for lotus root discrimination because it is mined 3-4 years old ginseng. According to the conventional belief that the older roots are more effective, the older roots are more expensive because of the higher price. Lotus root discrimination is a big problem.
  • the present inventors separate and analyze standard markers from Korean ginseng and Chinese ginseng, which are studying the origin or lotus root discrimination method of Korean ginseng and Chinese ginseng.
  • the present invention was completed by confirming that it is possible to determine a mountainous region or lotus root.
  • An object of the present invention is to provide a marker for producing white ginseng mountain tube discrimination used for mountain production of white ginseng.
  • Another object of the present invention is to provide a method for determining the production of white ginseng.
  • Another object of the present invention is to provide a marker for determining the red ginseng mountain region used for mountain determination of Heungsam.
  • Another object of the present invention is to provide a method for determining a mountain of Heungsam.
  • Another object of the present invention to provide a marker for determining the age of Heungsam used for determining the age of Heungsam.
  • Another object of the present invention is to provide a method for determining the age of red ginseng.
  • the present invention provides a marker for determining the origin of white ginseng represented by the following Chemical Formula 1 or 2, which is used to determine the origin of domestic white ginseng and Chinese white ginseng:
  • the present invention comprises the steps of extracting a metabolite from the test white ginseng (step 1);
  • the present invention provides a method for determining the production of white ginseng comprising the step (step 3) comparing the LC-MS analysis obtained in step 2 with the marker for determining the mountain region of Formula 1 or 2. Furthermore, the present invention provides a marker for determining a mountain area of Heungsam represented by the following Chemical Formula 3 or 4, which is used for mountain production of domestic red ginseng and Chinese heungsam:
  • the present invention comprises the steps of extracting a metabolite from the test red ginseng (step 1);
  • the present invention provides a method for determining a mountain of Heungsam, comprising the step (step 3) of comparing the LC-MS analysis result obtained in step 2 with the mountain determination marker of the formula (3) or (4). Furthermore, the present invention provides a marker for determining the age of red ginseng represented by the following Chemical Formulas 5 to 11 used for the lotus root discrimination of domestic four-year-old red ginseng and six-year-old red ginseng:
  • the present invention comprises the steps of extracting the metabolite from the test heungsam (step 1);
  • step 3 Comparing the LC-MS analysis result obtained in step 2 with the age determination marker selected from the group consisting of the compounds represented by the formula (5 to 11) (step 3) age determination method of red ginseng To provide.
  • a standard marker is established in domestically processed ginseng, that is, white ginseng and red ginseng, and the origin or age of processed ginseng such as concentrate or powder, which is difficult to identify the origin or age, can be quickly determined using the marker. As it is possible to compare and analyze, it can be useful for import and export of ginseng seedlings for determination of origin or age.
  • Figure 1 shows an ion chromatogram of domestic white ginseng extract of the embodiment of the present invention I am drawing. '
  • FIG. 2 is a diagram showing an ion chromatogram of Chinese ginseng extract of the embodiment of the present invention.
  • FIG. 3 is a diagram illustrating a core scatter plot of domestic white ginseng extract and Chinese white ginseng extract of an embodiment of the present invention.
  • Figure 4 is a view showing the S plot of the least orthogonal square analysis of domestic white ginseng extract and Chinese white ginseng extract of the embodiment of the present invention.
  • FIG. 5 is a diagram illustrating a box plot of (a) domestic white ginseng extract and (b) Chinese white ginseng extract of an embodiment of the present invention.
  • FIG. 6 is a view showing a mass cutting method of the domestic white ginseng extract of the embodiment.
  • FIG. 7 is a view showing a mass cutting method of the Chinese white ginseng extract of the embodiment.
  • FIG. 8 is a diagram showing an ion chromatogram of a domestic Heungsam extract of an embodiment of the present invention.
  • FIG. 9 is a view showing an ion chromatogram of Chinese red ginseng extract of the embodiment of the present invention.
  • FIG. 10 is a view of the domestic heungsam extract and Chinese heungsam extract of the embodiment of the present invention
  • FIG. 11 is a view showing an S plot of the least orthogonal square analysis of domestic red ginseng extract and Chinese red ginseng extract according to an embodiment of the present invention.
  • FIG. 12 is a diagram illustrating a box plot of (a) Korean red ginseng extract and (b) Heungsam extract made in China according to an embodiment of the present invention.
  • Figure 13 is a view showing the mass cutting method of the domestic red ginseng extract of the embodiment.
  • FIG. 14 is a view showing a mass cutting method of the Chinese heungsam extract of the embodiment.
  • FIG. 15 is a diagram illustrating an ion chromatogram of a domestic four-year-old Heungsam extract of an example of the present invention.
  • FIG. 16 is a diagram illustrating an ion chromatogram of Korean six-year-old red ginseng extract of the example of the present invention.
  • 17 is a four-year-old Heungsam extract and six-year-old red ginseng extract of the domestic embodiment of the present invention .
  • the score scatter plot of FIG. 17 is a four-year-old Heungsam extract and six-year-old red ginseng extract of the domestic embodiment of the present invention .
  • the score scatter plot of FIG. 17 is a four-year-old Heungsam extract and six-year-old red ginseng extract of the domestic embodiment of the present invention .
  • FIG. 18 is a diagram illustrating an S-plot of the least-orthogonal square analysis of domestic 4-year-old Heungsam extract and 6-year-old Red ginseng extract according to an embodiment of the present invention.
  • FIG. 19 is a diagram illustrating a box plot of the domestic 4-year-old Heungsam extract of the embodiment of the present invention.
  • 20 is a view showing a box plot of the domestic six-year-old red ginseng extract of the embodiment of the present invention.
  • the present invention provides a marker for determining the production site of white ginseng represented by the following formula (1) or 2 used for determining the production of domestic white ginseng and Chinese white ginseng:
  • the compound represented by the formula (1) is a marker for determining the origin of domestic white ginseng
  • the compound represented by the formula (2) is a marker for determining the origin of Chinese ginseng.
  • Marker for determining the production site of white ginseng represented by the formula (1) or 2 according to the present invention comprises the steps of extracting a metabolite from domestic white ginseng and Chinese white ginseng (step 1);
  • step 2 the result obtained by converting the result analyzed by LC—MS into a numerical value that can be statistically processed, and analyzing the converted value by multivariate analysis, detects a component that shows a difference between groups, and shows the largest difference.
  • a component that shows a difference between groups and shows the largest difference.
  • step 1 is a step of extracting metabolites from domestic white ginseng and Chinese white ginseng
  • the extraction solvent is preferably water, alcohol or a mixture thereof.
  • the alcohol it is preferable to use d to C 4 lower alcohol, more preferably to use ethanol or methanol, and most preferably to use 70% methane, but not always limited thereto.
  • the extraction method conventional methods in the art, such as filtration, hot water extraction, dipping extraction, reflux angle extraction, and ultrasonic extraction, can be used, and the extraction is preferably performed once to five times by ultrasonic extraction, and repeated three times. More preferably, but not limited to extraction.
  • the extraction temperature is preferably 20 to 30 ° C, but is not limited thereto.
  • step 2 is a liquid chromatography of the metabolite extracted in step 1
  • the mobile phase A was water and formic acid (100: 0.1, v / v)
  • the mobile phase B was analyzed by mass spectrometry (LC-MS).
  • LC-MS mass spectrometry
  • the flow rate of the mobile phase in minutes and the sample injection amount can be used for measurement by taking 1 sample of domestic white ginseng extract and 1 sample of Chinese white ginseng extract.
  • the mass spectrometer may use a quadrupole orthogonal accelerated flight time tandem mass spectrometer, but is not limited thereto.
  • step 3 converts the result analyzed by LC-MS into a numerical value capable of statistical processing, and analyzes the converted value by multivariate analysis to determine the difference between the data and the group. After detecting the visible components, selecting the components with the highest difference to establish standard markers of domestic white ginseng and Chinese white ginseng as standard markers according to the regions.
  • the multivariate analysis method used here is an orthogonal least square analysis method. The data obtained by analyzing the multivariate method can be used to identify standard markers using a score scatter plot.
  • It provides a method for determining the production of white ginseng comprising the step (step 3) comparing the LC-MS analysis result obtained in step 2 with the marker for determining the mountain region of Formula 1 or 2.
  • the white acid production method of white ginseng can be used to determine the origin of white ginseng during import and export, since it is possible to quickly compare white ginseng production areas such as concentrates and powders that are difficult to identify.
  • the present invention provides a marker for determining the production site of red ginseng represented by the following Chemical Formula 3 or 4, which is used to determine the production of domestic Heungsam and Chinese Heungsam:
  • the compound represented by the formula (3) is a marker for discriminating the production site of Korean red ginseng
  • the compound represented by the formula (4) is a marker for discriminating the production site of Chinese red ginseng.
  • the marker for determining the production site of red ginseng represented by the formula (3) or 4 according to the present invention comprises the steps of extracting a metabolite from domestic heungsam and Chinese heungsam (step 1);
  • the result analyzed by LC-MS in step 2 is converted into a numerical value that can be statistically processed, and the component having the difference between the groups is detected from the data obtained by analyzing the converted value by multivariate analysis, and the component having the largest difference is selected.
  • the steps 1 to 3 may be carried out in the same manner as the mountain irrigation method of the white ginseng, but is not limited thereto.
  • the present invention
  • red ginseng It provides a method for determining the production location of red ginseng comprising the step (step 3) comparing the LC-MS analysis obtained in step 2 with the mountain determination marker of the formula (3) or (4).
  • the production method of the red ginseng can be quickly compared with the use of standard markers, such as concentrated powder, which is difficult to identify the production area using a standard marker, so the import and export of red ginseng It can be useful for discrimination.
  • the present invention provides a marker for determining the age of red ginseng represented by the following Chemical Formulas 5 to 11 used for the discrimination of lotus root of four-year-old Heungsam and six-year-old Korean red ginseng:
  • the compound represented by the formula 5 to 8 is a marker for determining the age of 4 years old red ginseng
  • the compound represented by the formula 9 to 11 is a marker for determining the age of 6 years old red ginseng.
  • the age-determining marker of red ginseng represented by Chemical Formulas 5 to 11 according to the present invention includes the steps of extracting metabolites from domestic 4-year-old Heungsam and 6-year-old Heungsam (step 1);
  • step 2 the result analyzed by LC-MS is converted into a numerical value capable of being statistically processed, and the transformed value is analyzed by multivariate analysis to detect components showing differences between groups, and the largest difference is found. It can be established by a method comprising the step of selecting the ingredients and establishing them as standard markers according to the age of Heungsam.
  • the steps 1 to 3 may be performed in the same manner as the mountain determination method of the white ginseng, but is not limited thereto.
  • the method of determining age of Heungsam comprising the step of comparing the LC-MS analysis result obtained in step 2 with an age discriminating marker selected from the group consisting of the compounds represented by Chemical Formulas 5 to 11 (step 3).
  • the age determination method of red ginseng can be usefully used to determine the age of red ginseng at the time of import and export, since it is possible to quickly compare the age of Heungsam such as concentrated liquid and powder, which are difficult to identify by using a standard marker.
  • the present invention provides a kit for determining a mountain of white ginseng comprising a marker represented by Chemical Formula 1 or 2.
  • the present invention provides a kit for determining the location of red ginseng comprising a marker represented by the formula (3) or (4). Furthermore, the present invention provides a kit for determining the lotus root of domestic red ginseng comprising any one or more of the markers represented by the formulas (5) to (11).
  • Kits prepared according to the present invention can be usefully used to determine the origin of white ginseng or red ginseng, or to determine the lotus root of domestically grown ginseng.
  • red ginseng was prepared from 4 root 10 roots and 6 roots 10 roots of ginseng from Icheon, Gyeonggi-do.
  • Step 2 Particle Extract of White Ginseng Extract Using Liquid Chromatography-Mass Spectrometry (LC-MS)
  • UPLC used the Waters Acquisition UPLC System, and the column was the Equity TM BEHC 18 (ACQUITY).
  • UPLC TM BEHCi 8 (10 ⁇ X2.1 mm, id, 1.7ym, waters, USA) was used at 35 t, mobile phase A for water and formic acid (100: 0.1, v / v) and mobile phase B for aceto Initially increase mobile phase B from 10% to 30% for 7 minutes with nitrile (acetonitril) and formic acid (100: 0.1, v / v) and then move to mobile phase B up to 14 minutes at 44% and 21 minutes at 100%.
  • the mobile phase B was maintained at 10 to 23. minutes, the mobile phase B was reduced to 10% at 23.6 minutes, and after equilibrating, it was used for the next analysis.
  • the flow rate of the mobile phase was 0.4 minutes, and the sample injection amount of each sample of domestic white ginseng extract and Chinese white ginseng extract was used for each measurement.
  • the Q-T0F mass spectrometer used Waters' quadrupole orthogonal accelerat ion time of—flight tandem mass spectrometer (Waters Q-T0F Premier TM). Analysis conditions of the mass spectrometer are shown in Table 1 below.
  • FIGS. 1 and 2 The total chromatogram of liquid chromatography-mass spectrometry (LC-MS) of the domestic white ginseng extract and the Chinese white ginseng extract is shown in FIGS. 1 and 2.
  • LC-MS liquid chromatography-mass spectrometry
  • Step 3 Transformation into Statistical Processable Numbers and Establishment of the White Ginseng Standard Marker
  • peak finding and peak sorting are performed using a Markersynx applications manager (ver. 4.1) of Waters Corporation. peak alignment and peak filtering were performed.
  • the analytical mass range was 200-1500 Da
  • mass The tolerance range was 0.02 Da
  • the mass noise level was 1 ⁇ of the base peak intensity.
  • the retention time (RT) value of each peak coincides with the mass value, and the area value of each peak is obtained accordingly, and then Simca-P + ver for multivariate statistical analysis.
  • the standard marker of domestic white ginseng is 20-glucosinenoside Rf ( 5.21 minutes, MW 963.5582), the standard marker of Chinese white ginseng, as shown in the following formula 2, notojincetoside. R2 (9.30 min, M.W. 771.4919).
  • Step 2 Analysis of Red Ginseng Extract Using Liquid Chromatography-Mass Spectrometry (LC-MS)
  • Step 3 Statistical Processing and Establishment of Red Ginseng Standard Markers
  • Ginsenoside Rd 11.70 minutes, MW 767.4952.
  • Step 2 Determination of Red Ginseng Extract Using Liquid Chromatography-Mass Spectrometry (LC-MS)
  • Step 3 Transformation into Statistical Processable Numbers and Establishment of Red Ginseng Standard Markers
  • the four-year-old red ginseng component and the six-year-old heungsam component are shown in Table 2 below.

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Abstract

La présente invention porte sur un marqueur standard pour distinguer le pays d'origine ou l'âge de ginseng traité, sur son procédé d'établissement ou sur un procédé pour distinguer le pays d'origine ou l'âge de ginseng l'utilisant. Selon la présente invention, l'invention est apte à établir un marqueur standard pour du ginseng coréen traité, à savoir du ginseng blanc et du ginseng rouge, et peut être utile pour distinguer le pays d'origine ou l'âge du ginseng traité lorsque le ginseng traité est importé et exporté par comparaison et analyse rapides du pays d'origine ou de l'âge du ginseng traité à partir d'un concentré ou de poudre, et analogue, pour lequel le pays d'origine ou l'âge n'est pas facile à distinguer, à l'aide du marqueur.
PCT/KR2011/007368 2011-06-30 2011-10-05 Marqueur standard pour distinguer le pays d'origine ou l'âge de ginseng traité, son procédé d'établissement, ou procédé pour distinguer le pays d'origine ou l'âge de ginseng l'utilisant WO2013002455A1 (fr)

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KR10-2011-0064735 2011-06-30
KR1020110064735A KR101445303B1 (ko) 2011-06-30 2011-06-30 가공인삼의 원산지 또는 연령 판별용 표준 마커, 이의 확립방법 또는 이를 이용한 원산지 또는 연령 판별방법

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104133025A (zh) * 2014-02-26 2014-11-05 贾忠 一种蟅虫创愈制剂的检测方法
CN108593825A (zh) * 2018-01-24 2018-09-28 中国科学院大连化学物理研究所 红参质谱数据的挖掘与特异性标志物的筛选方法
CN111257485A (zh) * 2018-11-30 2020-06-09 中国科学院大连化学物理研究所 一种使用多肽标志物区分西洋参和人参的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101695615B1 (ko) * 2014-11-28 2017-01-13 대한민국 X―선 형광분석기를 이용한 홍삼 농축액의 원산지 판별방법
KR101629570B1 (ko) * 2014-12-02 2016-06-13 경희대학교 산학협력단 사중극자 비행시간 질량 분석법에 결합시킨 고속 분리능 액체 크로마토그래피를 이용한 고려인삼의 재배지 판별 방법
KR101965293B1 (ko) * 2016-08-29 2019-04-03 서울대학교산학협력단 한국산과 중국산 백미 판별용 Biomarker 및 이를 이용한 판별 모델

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060081390A (ko) * 2006-06-23 2006-07-12 조순길 당귀 원산지 식별 방법
KR20070027532A (ko) * 2004-03-17 2007-03-09 티안진 타슬리 파마슈티컬 컴퍼니 리미티드 심장-뇌 혈관성 질환용 한약 조제물 및 그의 제조 방법
KR100740582B1 (ko) * 2006-09-27 2007-07-19 한국과학기술연구원 가스크로마토그래피-질량분석기를 이용한 두 생체시료군 간대사체 차별성 분석 방법
KR100905414B1 (ko) * 2008-06-25 2009-07-02 대한민국 한약재 산지 판별방법
KR20100087092A (ko) * 2007-09-14 2010-08-03 알버트 와이. 릉 한약을 평가하기 위한 시스템 및 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070027532A (ko) * 2004-03-17 2007-03-09 티안진 타슬리 파마슈티컬 컴퍼니 리미티드 심장-뇌 혈관성 질환용 한약 조제물 및 그의 제조 방법
KR20060081390A (ko) * 2006-06-23 2006-07-12 조순길 당귀 원산지 식별 방법
KR100740582B1 (ko) * 2006-09-27 2007-07-19 한국과학기술연구원 가스크로마토그래피-질량분석기를 이용한 두 생체시료군 간대사체 차별성 분석 방법
KR20100087092A (ko) * 2007-09-14 2010-08-03 알버트 와이. 릉 한약을 평가하기 위한 시스템 및 방법
KR100905414B1 (ko) * 2008-06-25 2009-07-02 대한민국 한약재 산지 판별방법

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104133025A (zh) * 2014-02-26 2014-11-05 贾忠 一种蟅虫创愈制剂的检测方法
CN108593825A (zh) * 2018-01-24 2018-09-28 中国科学院大连化学物理研究所 红参质谱数据的挖掘与特异性标志物的筛选方法
CN108593825B (zh) * 2018-01-24 2020-07-07 中国科学院大连化学物理研究所 红参质谱数据的挖掘与特异性标志物的筛选方法
CN111257485A (zh) * 2018-11-30 2020-06-09 中国科学院大连化学物理研究所 一种使用多肽标志物区分西洋参和人参的方法
CN111257485B (zh) * 2018-11-30 2022-02-15 中国科学院大连化学物理研究所 一种使用多肽标志物区分西洋参和人参的方法

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