WO2012146169A1

WO2012146169A1 - Chinese herbal extract, preparing method and application thereof

Info

Publication number: WO2012146169A1
Application number: PCT/CN2012/074665
Authority: WO
Inventors: 赵亚; 方程; 祝乐; 陈默; 程康; 熊卫国; 尹哲芳
Original assignee: 上海家化联合股份有限公司
Priority date: 2011-04-26
Filing date: 2012-04-25
Publication date: 2012-11-01
Also published as: AU2012247916A1; CN102755266A

Abstract

A Chinese herbal composition with anti-aging effect is characterized in that it comprises the water and/or solvent extracts of the following raw materials in weight parts: 30 to 70 parts of Rhizoma Polygonati, 30 to 70 parts of Rhizoma Polygonati Odorati, 0.01 to 5 parts of gold foil. The said solvent is selected from methanol, ethanol, isopropyl alcohol, n-butanol, ethylene glycol, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol, acetone, ethyl acetate, or the combination thereof. The extract of Chinese herbal the and the Chinese herbal composition comprising it possess the effects of moisturizing skin, repairing, caring and rebirthing, without stimulus to skin. They are used in skin anti-aging, or in cosmetics as active additives, and its cost is lower.

Description

Chinese herbal medicine extract and preparation and application thereof

The invention relates to the field of cosmetics, in particular to a Chinese herbal medicine extract, a preparation thereof and an application thereof, and an anti-aging Chinese herbal medicine composition comprising the Chinese herbal medicine extract, and preparation and application thereof. Background technique

At present, most anti-aging cosmetics at home and abroad support biochemical preparations and synthetic compounds as main additives. With the requirements of natural, safety and environmental protection, green cosmetics are increasingly favored by people. However, the existing cosmetics on the market, which are supplemented with natural products or plant extracts, are based on pharmacological studies of single-flavored drugs. There is no guidance from the theory of traditional Chinese medicine, and there is a lack of compound research. Moreover, the anti-aging cosmetics of Chinese herbal medicines on the market are mostly simple combinations of single-flavored Chinese herbal extracts, without the guidance of TCM theory; or the extraction process is complicated, and the cost is increased. Summary of the invention

The technical problem to be solved by the present invention is to provide a Chinese herbal medicine extract having an anti-aging effect.

In order to achieve the above object, the Chinese herbal medicine extract of the present invention is a water and/or solvent extract of the following Chinese herbal medicines: water extract or solvent extraction of raw materials composed of Huangjing 30-70, Yuzhu 30-70 and gold foil 0.01~5. The solvent is selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1, 3-propanediol, 1, 3-butanediol, acetone, ethyl acetate or Its combination.

The "Huang Jing" used in the present invention is a dried rhizome of Polygonatum kingianum Coll. et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua., also known as tiger ginger and chicken. Head ginseng. According to the differences in the properties of the original plants and herbs, Huang Jing can be divided into three types: ginger-shaped yellow essence, chicken head yellow essence and rhubarb essence. The original plant of the ginger-shaped yellow essence is more yellow, the original plant of the yellow head of the chicken head is the yellow essence, and the original plant of the rhubarb essence (also known as the dish-shaped yellow essence) is the yellow essence. Among the three, the quality of ginger-shaped yellow essence is the best. Huang Jing is distributed in the northern provinces of China and Anhui, Zhejiang and Gansu, and the former Soviet Union. Polygonatum is mainly distributed in the southern provinces of China, Shaanxi, Gansu and the southeastern part of the Northeast. Quercus chinensis is distributed in Guizhou, Yunnan, Guangxi, Sichuan and other places in China.

Huang Jing is sweet and flat. It can nourish kidney and lungs, replenish spleen and replenish qi, and has anti-hypoxia, anti-fatigue and anti-aging Old role; at the same time can enhance immune function, improve metabolism; contains aspartic acid, foxglycoside, terpenoids, mucus, sugar, niacin and other active ingredients. There is a yellow essence cream in the "Prepare for the urgency", which can make "old skin off, color change, color is different, eyebrows must be changed".

The dried rhizome of Polygonatum odoratum (Mill.) Druce used in the present invention is native to southwestern China, but has a wide wild distribution. It is cold-tolerant, also resistant to yin, and is humid. Suitable for growing in loose soils rich in humus.

Yuzhu is sweet and flat. It can nourish the kidney and moisten the lungs, and replenish the spleen The same is Liliaceae. "Shen Nong's Herbal Classic" is listed as the medicine of the top grade, which means that it is "long-wearing to face black, good color, moisturizing". Containing polysaccharides, bio-alkali, cardiotonic, vitamin A and other biologically active nutrients, it is a classic oral and topical cosmetic product.

In the application of traditional Chinese medicine, gold foil can be added into medicine to cook decoction, or as a coating of pills, similar to the outer coating of modern western medicine. The most famous Angong Niuhuang Pill is a medicine with gold foil as a medicine. Gold to enhance the tranquillity of drugs. "Taiping Shenghui Fang", "Qian Jin Yao Fang" and "Sheng Ji General Record" have records of edible gold foil treatment. In addition, when acupuncture is used, acupuncture needles made of pure gold or alloy have a better function of guiding blood circulation. Modern pharmacological research: Polygonatum polysaccharide is one of the main active ingredients of Polygonatum, which has anti-tumor, anti-aging, immune regulation and anti-oxidation damage. The pharmacological effects of Polygonatum odoratum are mainly manifested in anti-aging, improving the body's immune system, lowering blood sugar, using blood lipids, and fighting tumors. According to the test conducted by the Japanese University of Tochigi with the water extract of Polygonatum odoratum, the skin has a significant aging-inhibiting effect and has a beautiful skin effect. Polygonatum odoratum polysaccharides enhance the ability to scavenge free radicals, inhibit lipid peroxidation, reduce malondialdehyde, and reduce damage to body tissues to delay aging.

The invention also provides a preferred preparation method of a Chinese herbal medicine extract, the method comprising:

The raw material consisting of the following parts by weight: Huang Jing: 30~70, Yuzhu 30~70 is extracted with water and/or solvent selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1 , 2 -propylene glycol,

1, 3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof;

Preferably, the method further comprises:

The extract is precipitated with alcohol, and the precipitate is added with water to dissolve, and then filtered; the filtrate is formulated into a solution.

Preferably, the extraction is by decoction, reflux extraction, dipping or osmosis.

Preferably, the alcohol precipitation is carried out using an alcohol selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1, 3-propanediol, 1,3-butanediol or a combination thereof. , preferably ethanol.

Preferably, after the precipitate is dissolved in water, it is purified by membrane separation dialysis method; the membrane is separated and dialyzed, Formulated as a solution.

Preferably, ultrafiltration can be employed during membrane separation dialysis.

Preferably, the membrane pore size used in the ultrafiltration is 100,000 Daltons.

The herbal extract of the present invention has an anti-aging effect and can be used in cosmetics.

The present invention also provides a Chinese herbal medicine composition having an anti-aging effect comprising the above-mentioned Chinese herbal medicine extract.

The Chinese herbal medicine extract of the invention has the functions of emollient, repairing, protecting and nascent, has no irritation to the skin, can be directly used for skin anti-aging, can also be used as an efficacy additive to be added into cosmetics, and has low cost. detailed description

The invention will now be described in greater detail by way of specific examples.

Example 1

Take pure Chinese herbal medicine Huangjing 30g, Polygonatum 70g total 100g, mix well, add 10 times the amount (multiple amount: refers to the ratio of solvent volume to the total weight of the medicine, where the unit volume of the solvent is ml, the unit of total weight of the medicine is _g , The same water is decoction and extracted for about 1 hour, the filtrate is taken, and the dregs are further decocted with 10 times of water for 1 hour. The filtrate is taken and combined with the former filtrate, concentrated, and the filtrate is added with an appropriate amount of ethanol, and allowed to stand for 24 hours; After the precipitation and water dissolution, the membrane separation equipment (equipment model: R F0460 roll membrane, membrane pore size: 100,000 Dalton, supplier: Xiamen Shida Membrane Technology Co., Ltd.) is purified, and the membrane dialysate is made into a crude drug. A solution of 500 mg/ml was obtained to obtain Extract A. Example 2

Take pure Chinese herbal medicine Huangjing 70g, Polygonatum 30g total 100g, mix well, add 10 times 70% ethanol reflux extraction for about 1 hour, leave the filtrate, add 10 times the amount of 70% ethanol reflux extraction for 1 hour, take the filtrate And combined with the former filtrate, the ethanol is recovered to dryness; the obtained extract is dissolved in water, filtered, and formulated into a solution containing a crude drug amount of 500 mg/ml to obtain an extract B. Example 3

Take pure Chinese herbal medicine Huangjing 40g, Polygonatum 60g total 100g, mix well, add 10 times the amount of 30% propylene glycol impregnation and extract for 24 hours, leave the filtrate, add 10 times the amount of 30% propylene glycol aqueous solution for 24 hours, filter, Recover part of the water to make a Chinese herbal medicine solution containing 500mg/ml of crude drug. Example 4

Take pure Chinese herbal medicine Huangjing 30g, Polygonatum 70g total 100g, mix well, add 10 times the amount of water to cook for about 1 hour, leave the filtrate, add 10 times of water to the dregs and boil for 1 hour, take the filtrate, and The former filtrates were combined, concentrated, and the filtrate was added with an appropriate amount of ethanol, and allowed to stand for 24 hours. After filtration, the precipitate was dissolved in water to prepare a solution containing a crude drug amount of 500 mg/ml to obtain an extract D. Experimental example 1

DPPH experiment:

Free radicals are a class of chemical substances that are active and highly oxidizing. Unstable free radicals generated during the metabolism of the body accumulate in the cells, causing the oxidation of unsaturated fatty acids into peroxides, forming lipofhscin (LF), and allowing cells and their important components such as DNA, proteins and enzymes. Change or destroy, leading to aging of the body. Reducing the damage of free radicals to the body can play an anti-aging role. DPPH is an important method for evaluating the ability of samples to scavenge free radicals and antioxidants.

The extracts in the examples were each diluted to 0.2%, 1%, 5% and 25% (v/v) concentrations with distilled water. Then, 2 ml of the extract of the example, 0.5 ml of a DPPH ethanol solution having a concentration of 500 μm, and 1.5 ml of water were added to the same test tube, shaken, and allowed to stand. After 30 minutes, the absorbance (OD) was measured at 517 nm, and The corresponding mixture was used as a blank control. The clearance of DPPH from the extracts of the examples was calculated according to the following formula:

T - TO

Clearance rate (%) = 1 - · * 100 %

C where:

Absorbance of T-DPPH+ Example Extract

T0 - Example Extract + Solvent Absorbance:

The absorbance of C-DPPH+ solvent. The antioxidant activity evaluation results of the Chinese herbal medicine extracts and compositions obtained in the respective examples are shown in Table 1 Concentration 5% 1% 0.2% Free radical scavenging rate (%) Chinese herbal extract A of Example 1 43.8% 27.1% 5.1% Chinese herbal extract B of Example 2 42% 23.6% 5.2% Chinese herbal extract C of Example 3 41.8% 22.2% 4.9% The Chinese herbal extract D of Example 4 D 43.5% 24.2% 6.3% The actual-risk results showed that the extracts of Examples 1 to 4 were better in the concentrations of 0.2%, 1% and 5% in vitro. The free radicals have the antioxidant capacity, wherein 5% of the free radical scavenging rate is above 40%, and the free radical scavenging rate of 1% concentration is above 20%. The extract A of Example 1 works best. Experimental example 2

Fibroblast proliferation test:

Fibroblasts in the skin are the main cells for the synthesis of collagen. The proliferation of fibroblasts or the enhancement of the ability of fibroblasts to synthesize and secrete collagen can increase the total amount of collagen and slow down the aging of the skin. Therefore, research into the proliferative effect of fibroblasts is one of the important methods for evaluating the anti-aging activity of samples. This test is based on the above mechanism of skin aging, and the method of in vitro culture of human fibroblasts is used to initially evaluate the anti-aging effect of the traditional Chinese medicine composition of the present invention.

Example The cell suspension was prepared (formulated at a concentration of 5 * 10 ³ / ml) were seeded in 96-well plates. After the adherent cells, in addition to the control group, other appropriate concentration was added to each well of μ 1 20 Chinese herbal extracts, set in 4 concentration groups, 6 wells in each group, incubate in incubator (37 ° C, 5% C0 ₂ ) for 48 h, then add MTT solution 20 μ 1 and continue in the incubator (37 Incubate for 4 h at °C, 5% C0 ₂ ). After removing the microplate, carefully aspirate the culture solution in each well, add DMSO, and mix well. The optical density (570 nm) was measured by enzyme-linked immunosorbent assay, and the relative proliferation rate of the sample group was obtained by comparison with the control group.

The results of the proliferative activity of the anti-aging Chinese herbal composition against fibroblasts are shown in Table 2.

Table 2

Concentration 1% 0.1% 0.01% 0.001%

Promote skin fibroblast growth rate (%)

Chinese herbal extract A of Example 1 A 31.0% 28.7% 28.6% 25.0% Chinese herbal extract B of Example 2 27.7% 21% 17.8% 12.5% Chinese herbal extract C of Example 3 25.6% 21% 15.6% 10.1% Chinese herbal extract D of Example 4 29.3% 25.4% 20.0% 13.2% Experimental results It is shown that the Extract A of Example 1, the extract of Example 2, and the extract of Example 3 (and the extract D of Example 4 can promote the proliferation of fibroblasts, and the extract of Example 1 has the best effect. Its 0.001% concentration contributes to the proliferation of fibroblasts by 25%.

The above experimental studies show that the Chinese herbal medicine composition of the present invention has the anti-oxidation ability of scavenging free radicals in vitro, can promote the proliferation of skin fibroblasts, and helps resist aging.

Claims

Claim

A Chinese herbal medicine extracting composition, characterized in that it comprises an extract of water and/or a solvent of the following parts by weight of raw materials: xanthan 30-70, polygonatum 30-70 and gold foil 0.01-5, the solvent is selected from the group consisting of 曱Alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1, 2-propanediol, 1,3-propanediol, 1, 3-butanediol, acetone, ethyl acetate or a combination thereof.

The Chinese herbal medicine extract according to claim 1, wherein the extraction is selected from the group consisting of boiling, reflux extraction, impregnation, percolation, or a combination thereof.

3. A method for preparing a Chinese herbal medicine extract, comprising the following steps:

The raw material consisting of the following parts by weight: Huang Jing: 30-70, Polygonatum 30-70 is extracted with water and/or solvent selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1, 2-propanediol, 1, 3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof;

4. The method of claim 3, further comprising:

The extract is added with alcohol to precipitate, and the precipitate is added with water to dissolve, and then filtered;

The filtrate is formulated into a solution.

5. The method according to claim 4, wherein the alcohol used for the alcohol precipitation is selected from the group consisting of: methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1, 3- An alcohol of propylene glycol, 1,3-butanediol or a combination thereof, preferably ethanol.

6. The method of claim 4 or 5, wherein

After the precipitate is dissolved in water, it is purified by membrane separation and dialysis;

The membrane was separated and dialyzed to prepare a solution.

The method for preparing a Chinese herbal medicine extract according to any one of claims 3 to 6, wherein the extraction is selected from the group consisting of decoction, reflux extraction, impregnation, percolation, or a combination thereof.

The method for preparing a Chinese herbal medicine extract according to any one of claims 3 to 7, wherein the membrane separation dialysis is ultrafiltration.

9. A herbal extract prepared by the method of any of claims 3-8.

A Chinese herbal medicine composition having an anti-aging effect, comprising the herbal extract of claim 1 or 2.

A Chinese herbal medicine composition having an anti-aging effect, comprising the herbal extract of claim 8.

12. Use of a Chinese herbal medicine extract according to claim 1, 2 or 9 in cosmetics.

13. The use according to claim 12, wherein the cosmetic is a cosmetic cream, an eye cream, a facial mask, a facial cleanser, a toner or a serum.