WO2012127559A1 - 新品種、植物品種の鑑別方法、及びイネ個体を早生化する方法 - Google Patents
新品種、植物品種の鑑別方法、及びイネ個体を早生化する方法 Download PDFInfo
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- WO2012127559A1 WO2012127559A1 PCT/JP2011/056551 JP2011056551W WO2012127559A1 WO 2012127559 A1 WO2012127559 A1 WO 2012127559A1 JP 2011056551 W JP2011056551 W JP 2011056551W WO 2012127559 A1 WO2012127559 A1 WO 2012127559A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Definitions
- the present invention relates to a new cultivar produced by a non-genetic recombination method, a method for distinguishing the new cultivar, and a method for premature rice plants.
- a group that belongs to the same species, but has a different genetic composition, and is different from other groups in a certain trait is called a breed. That is, even if it is the same kind of plant, the difficulty of cultivation, resistance to pest damage, yield, quality, and the like differ depending on the variety. For this reason, in crops, especially major crops such as rice and wheat, varieties have been improved to obtain better varieties since ancient times. In recent years, not only seed companies, but also countries and prefectures. It has also been actively conducted in the public institutions.
- genes of various plants such as Arabidopsis, rice, and wheat have been analyzed, and the obtained gene information has been disclosed.
- Many of these varieties have been improved by introducing a gene of a foreign species by a genetic recombination method using the disclosed gene information.
- an Hd1 gene encoding a plant-derived protein having a function of increasing plant photosensitivity, a method for producing a transformed plant into which the Hd1 gene has been introduced, and the like have been disclosed (for example, see Patent Document 1).
- breeding by genetic recombination has the advantage that a trait of a distantly related species that cannot normally be crossed can be introduced, there is a problem that its safety verification is not always sufficient.
- Patent Document 2 when a foreign useful chromosome fragment is replaced by a non-genetic recombination method, the substitution region of the introduced foreign variety-derived chromosome fragment is controlled, and the preferred character of the original variety is changed. Without disclosing a method for producing a new variety having a target trait.
- Patent Document 2 describes a new rice variety Koshihikari eichi 3 in which only the region containing the Hd1 gene of Habataki was introduced into Koshihikari by the method for producing this new variety.
- Koshihikari which has a wider cultivatable area, is desired.
- Koshihikari has a better taste than other varieties and is preferred by consumers.
- rice farmers prefer to cultivate Koshihikari even in areas that are not necessarily suitable for cultivating Koshihikari.
- Koshihikari is cultivated in the southern region, the heading time is too early and a sufficient yield cannot be expected.
- the high taste continues during the heading season, which reduces the taste.
- Koshihikari when Koshihikari is cultivated in the northern region, it becomes late growth, and even if it can be headed, ripening becomes poor due to low temperature, and rice cannot be harvested.
- Koshihikari is only suitable for cultivation within the range of 35.5 to 38.5 degrees north latitude of Japan. If there is Koshihikari that can be cultivated in an area north of 38.5 degrees north latitude, for example, Koshihikari can be harvested even in areas that could not be cultivated conventionally, such as Hokkaido.
- the present invention has an object to provide a new rice cultivar that can be cultivated in the northern region of the past and a method for quickly growing a rice individual.
- the present inventor has found that a chromosome fragment of a specific region existing on the third chromosome of rice variety Habataki and a chromosome of a specific region existing on the sixth chromosome of rice variety Habataki.
- the fragment By substituting the fragment with the rice cultivar Koshihikari, it was found that it was possible to grow early enough to be cultivated in the northern region more than before, and the present invention was completed.
- the present invention (1) Rice cultivar Koshihikari Kazusa 6 (Oryza sativa L. cultivar Koshihikari-kazusa6 gou), whose cultivar registration application number is No. 25587, (2) A progeny individual obtained by mating two individuals selected from the group consisting of an individual of the variety described in (1) and a progeny individual of the individual of the variety described in (1), (3) A method for discriminating whether a rice individual is a specific variety,
- the SNP corresponding to the 31st, 521, and 442nd SNP (single nucleotide polymorphism) in the 3rd chromosome of rice cultivar Nipponbare is A DNA in rice cultivar Koshihikari and C in rice cultivar Habataki as DNA marker M1
- the SNP corresponding to the 31st, 689th, and 690th SNPs of chromosome 3 of rice cultivar Nipponbare (C for rice cultivar Koshihikari and T for rice cultivar Habataki) is defined as
- SNP corresponding to the 8th, 757, and 818th SNP of chromosome 6 of rice cultivar Nipponbare (C for rice cultivar Koshihikari and T for rice cultivar Habataki) is defined as DNA marker M1
- the SNP corresponding to the 8,940,503rd SNP of chromosome 6 of rice cultivar Nipponbare (A for rice cultivar Koshihikari and G for rice cultivar Habataki) is used as DNA marker M2
- the SNP corresponding to the 9,325,062nd SNP of chromosome 6 of rice cultivar Nipponbare (C for rice cultivar Koshihikari and G for rice cultivar Habataki) is defined as DNA marker M3
- the SNP corresponding to the 9,533,057 SNP of chromosome 6 of rice cultivar Nipponbare (G for rice cultivar Koshihikari and C for rice cultivar Habataki) is used as DNA marker M4, SNP corresponding to 9,777
- chromosomal fragment consisting of the region, a method for premature rice cultivation, (6)
- the upstream end of the chromosome fragment is present in a region corresponding to a region including the 31st, 689th, 691st base to the 31st, 720, 064th base of the third chromosome of rice cultivar Nipponbare, and
- the chromosomal fragment is present so that the downstream end of the chromosomal fragment is present in a region corresponding to the region from the 31st, 724, 043th base to the 32,298, 685th base of the third chromosome of rice cultivar Nipponbare.
- a method of prematurely cultivating a rice individual according to the above (5) characterized by comprising: (7) A region corresponding to the region from the 31st, 689th and 690th bases to the 32th, 298th and 686th bases in the 3rd chromosome of the rice variety Nipponbare in the 3rd chromosome of the rice individual Koshihikari Kazusa No.
- chromosomal fragment consisting of the region, a method for premature rice cultivation, (8)
- the upstream end of the chromosomal fragment is present in a region corresponding to the region from the 31st, 521, 443th base to the 31st, 689th, 690th base of the third chromosome of rice cultivar Nipponbare.
- the downstream end of the chromosome fragment is present in a region corresponding to the region from the 32,298,686th base to the 32,363,156th base of the third chromosome of rice cultivar Nipponbare.
- a method for rapidly growing a rice individual according to (7), wherein a chromosome fragment is substituted (9) A rice cultivar produced by the method for rapidly growing a rice individual according to any one of (5) to (8), (10) A progeny individual obtained by mating two individuals selected from the group consisting of an individual of the variety described in (9) and a progeny individual of the individual of the variety described in (9), (11) A region corresponding to the region from the 8,940,503th base to the 9,533,057th base in the 6th chromosome of the rice variety Nipponbare in the 6th chromosome of the rice individual Koshihikari Kazusa No. 6, Rice Variety Koshihikari Eichi No.
- the present invention provides a rice cultivation method characterized by cultivating one or more rice individuals selected from the group consisting of rice individuals in the north of 38.5 degrees north latitude.
- rice cultivar Koshihikari Kazusa No. 6 is a new variety that is almost equivalent to Koshihikari in characteristics other than the harvest period such as quality and yield.
- the rice variety Koshihikari kazusa No. 6 can be identified by the method for identifying rice varieties of the present invention.
- the rice individual of the present invention can be made to grow faster than the original cultivar by the method for making the rice individual grow faster.
- FIG. 3 is a diagram showing a target region T on a chromosome G of an original variety, a chromosome segment L derived from a foreign variety that has been replaced, and DNA markers M1 to M5. It is the figure which represented typically the genome of Koshihikari and Koshihikari eichigo No.5. It is the figure which showed the result of having investigated the heading time of Koshihikari and Koshihikari eichi No. 5 in Chiba Prefecture. It is the figure which showed the result which investigated the heading time of Koshihikari and Koshihikari eichi No. 5 in Hokkaido. It is the figure which represented typically the genome of Koshihikari eichi 3go.
- the chromosome fragment replacement line means a line in which only a part of the chromosome of the original variety is replaced with a chromosome fragment derived from a foreign variety.
- the foreign cultivar is not particularly limited as long as it is a cultivar other than the original cultivar. It may be a variety other than plants such as animals.
- the cultivar means a group that can be clearly distinguished from other varieties in the same species in a certain trait because the plants are of the same species and have different genetic constitutions.
- the DNA marker is not particularly limited as long as it can detect the difference in the DNA sequence on the chromosome that can distinguish the chromosome derived from the original variety and the chromosome derived from the foreign variety.
- DNA markers can be used.
- the DNA marker may be, for example, a marker capable of detecting a genetic polymorphism such as SNP (Single Nucleotide Polymorphism, single gene polymorphism) or SSR (Simple Sequence Repeats, simple repeat sequence), and the like.
- RFLP Restriction Fragment Length Polymorphism, restriction enzyme fragment length polymorphism
- discrimination between the original variety-derived alleles and the foreign variety-derived alleles by these DNA markers can be performed by a conventional method. For example, using DNA extracted from each individual as a template, performing PCR using a primer that can specifically hybridize with a specific SNP or SSR, detecting the presence or absence of a PCR product using electrophoresis or the like, Polymorphism can be identified. In addition, after DNA extracted from each individual is treated with a restriction enzyme, the pattern of the DNA fragment can be detected using electrophoresis or the like to identify each polymorphism.
- a primer that can specifically hybridize with a specific SNP or SSR can be designed by a conventional method using a commonly used primer design tool or the like according to the base sequence of the SNP or SSR.
- designed primers and the like can be synthesized using any method well known in the art.
- DNA markers known DNA markers can be used as appropriate. Moreover, the DNA marker produced newly may be sufficient. As known DNA markers, for example, in rice, the SNP marker disclosed in International Publication No. 2003/070934, etc., Rice Genome Research Program (RGP: http://rgp.dna.affrc.go.jp) /Publicdata.html) publicly available DNA markers can be used.
- RGP Rice Genome Research Program
- the gene information of each variety can be obtained from, for example, NCBI (National center for Biotechnology Information) and DDBJ (DNA Data Bank of Japan), which are international base sequence databases.
- the genetic information of each rice variety can be obtained from KOME (Knowledge-based Oryza Molecular biologic Encyclopedia, http://cdna01.dna.affrc.go.jp/cDNA/).
- the region from the Xth base to the Yth base of the chromosome of rice cultivar Nipponbare is the nucleotide sequence of the genomic DNA of rice cultivar Nipponbare published in RGB (version 4; IRGSP- This is an area determined based on build4-06 / 04/21).
- the region corresponding to the region from the Xth base to the Yth base of the chromosome of rice cultivar Nipponbare refers to the chromosome of the rice cultivar Nipponbare in the chromosome of the rice individual. This region is highly homologous to the region, and can be determined by aligning the known genomic DNA of the rice cultivar Nipponbare and the base sequence of the genomic DNA of the rice individual so as to have the highest homology.
- SNPs corresponding to SNPs of rice varieties Nipponbare” in rice individuals other than rice varieties Nipponbare include the known genomic DNA of rice varieties Nipponbare and the genomic DNA of the rice individual in the region containing the SNP. , When aligned so that the homology is highest, it means a base at a position corresponding to the SNP.
- the inventors of the present invention In order to breed new varieties that can be cultivated in a region north of conventional varieties, the inventors of the present invention first crossed rice varieties Habataki and rice varieties Koshihikari with respect to the heading period, and QTL ( Quantitative Trait Locus) analysis was performed. As a result, the QTS14 region of the long arm of chromosome 3 of rice cultivar Habataki [region corresponding to the region from the 31st, 720, 064th base to the 31st, 724, 043th base of the 3rd chromosome of rice cultivar Nipponbare] It was also found that there is a QTL for early heading and early birth. Therefore, the present inventor has produced a new variety in which a gene contained in the region of Koshihikari is replaced with a gene derived from Habataki. The new variety was expected to be an earlier rice than the original variety Koshihikari.
- the method for producing a new variety described in Patent Document 2 is specifically as follows. First, five types of DNA markers having a positional relationship as shown in FIG. 1 were set based on known rice gene information. That is, the DNA marker M2 is upstream of the target region T or upstream thereof, the DNA marker M1 is upstream of the DNA marker M2, the DNA marker M4 is downstream of the target region T or downstream thereof, and the DNA marker M4 is downstream. DNA marker M5 is set in the target region T, and DNA marker M3 is set in the target region T.
- a progeny individual having a side end between the DNA markers M1 and M2 and a downstream end of the region between the DNA markers M4 and M5 can be obtained.
- the DNA markers M1 and M5 are the same type as the original variety, and the DNA markers M2, M3, and M4 are the same type as the foreign variety (in the present invention, Habataki). .
- the distance d1 between the DNA markers M1 and M2 is long, the upstream end of the foreign-variety-derived chromosome fragment (in this application, Habataki-derived chromosome fragment) L exists.
- the range to be obtained is wide, and the length of the introduced Habataki-derived chromosome fragment L is difficult to determine.
- the distance d1 is short, the range in which the upstream end of the Habataki-derived chromosome fragment L can exist is narrow, and the length of the Habataki-derived chromosome fragment L to be introduced is easily determined.
- the distance d3 between the DNA markers M4 and M5 is long, the range in which the downstream end of the Habataki-derived chromosome fragment L can exist is wide, and the length of the Habataki-derived chromosome fragment L to be introduced becomes difficult to determine. If d3 is short, the range in which the downstream end of Habataki-derived chromosome fragment L can exist is narrow, and the length of Habataki-derived chromosome fragment L to be introduced is easily determined.
- C in the rice cultivar Habataki is the DNA marker M1 (DNA marker M1-Ac (QTS14))
- the SNP corresponding to the 31st, 689th, and 690th SNPs in the third chromosome of the rice cultivar Nipponbare (C in the rice cultivar Koshihikari In the rice variety Habataki, T) is the DNA marker M2 (DNA marker M2-Ct (QTS14))
- the SNP corresponding to the 32,208,924th SNP of chromosome 3 of the rice variety Nipponbare A, in the rice variety Koshihikari, A, In rice varieties Habataki, G) is replaced with DNA marker M3 (DNA marker M
- SNP corresponding to the 32,298,686th SNP of chromosome 3 of rice cultivar Nipponbare is DNA marker M4 (DNA marker M4-Gc (QTS14))
- the SNP corresponding to the 32,363,157th SNP of chromosome 3 of rice cultivar Nipponbare was used as DNA marker M5 (DNA marker M5-At (QTS14)).
- the region from the DNA marker M2-Ct (QTS14) to the DNA marker M4-Gc (QTS14) in the chromosome 3 of the rice individual that is, 31,689,
- a new cultivar was produced in which the region including the region from the 690th base to the 32,298,686th base) was replaced with a Habataki-derived chromosome fragment.
- the upstream end of the Habataki-derived chromosome fragment is located downstream from the DNA marker M1-Ac (QTS14) and up to the DNA marker M2-Ct (QTS14) (ie, the third chromosome of the rice cultivar Nipponbare).
- the region from the 31st, 521, 443th base to the 31st, 689th, 690th base) and the downstream end of the chromosome fragment is from the DNA marker M4-Gc (QTS14) to the DNA marker
- a region upstream from M5-At (QTS14) ie, a region corresponding to the region from the 32,298,686th base to the 32,363,156th base in the third chromosome of rice cultivar Nipponbare) Exists.
- FIG. 2 is a diagram schematically showing the genomes of Koshihikari and Koshihikari eichi No.5.
- the heading time of Koshihikari eichi No. 5 was measured (seeding date: May 6, 2010, transplanting date: June 1, 2010).
- the heading period was from August 5 to August 8, whereas Koshihikari Eichi No. 5 was from July 24 to July 26.
- Koshihikari Eichi No. 5 was clearly found to be early born compared to the original variety Koshihikari.
- Koshihikari Eichi No. 5 was measured in a field in Hokkaido north of 38.5 degrees north latitude (seeding date: April 28, 2010, transplanting date: June 7, 2010). As a result, as shown in FIG. 4, the heading period of Koshihikari was from September 1 to September 2, whereas Koshihikari eichi No. 5 was from August 21 to August 22. . In other words, even when cultivated in Hokkaido, Koshihikari Eichi No. 5 was clearly found to be early growing compared to the original variety Koshihikari. However, although Koshihikari and Koshihikari eichi No. 5 both headed, they did not mature sufficiently and could not harvest rice.
- the inventor of the present invention may be able to obtain Koshihikari that is early enough to be cultivated even in the northern region by stacking foreign chromosome fragments having an early-emergence function on Koshihikari eichi-5. Thought. Therefore, Koshihikari Eichi No. 5 and the rice variety Koshihikari Eichi No. 3 were crossed.
- Koshihikari eichi 3 is a variety in which only the region containing the Hd1 gene of chromosome 6 in Koshihikari's chromosome is replaced with a gene fragment derived from Habataki by the method for producing a new variety described in Patent Document 2.
- Table 2 shows five types of DNA markers used for the production of Koshihikari eichi 3go.
- the DNA marker M1-Ct is an SNP corresponding to the 8,757,818th SNP of chromosome 6 of the rice cultivar Nipponbare (C for the rice cultivar Koshihikari and T for the rice cultivar Habataki), and the DNA marker M2-Ag is The SNP corresponding to the 8,940,503rd SNP of chromosome 6 of rice cultivar Nipponbare (A in rice cultivar Koshihikari, G in rice cultivar Habataki), and DNA marker M3-Cg is chromosome 6 of rice cultivar Nipponbare Is the SNP corresponding to the 9,325,062nd SNP (C for rice cultivar Koshihikari and G for rice cultivar Habataki), and the DNA marker M4-Gc is the 9,533,057th chromosome 6 of rice cultivar Nipponbare SNP (G for rice cultivar Koshihikari, C for rice cultivar Habataki), and DNA marker M5 At is a SNP corresponding to 9,777,196 th S
- Koshihikari eichi 3 is a region from the DNA marker M2-Ag (Hd1) to the DNA marker M4-Gc (Hd1) in the chromosome 6 of the rice variety Koshihikari (that is, in the chromosome 6 of the rice variety Nipponbare).
- a region including the region from the 8,940,503rd base to the 9,533,057th base) is a new variety in which a Habataki-derived chromosome fragment is replaced.
- the upstream end of the Habataki-derived chromosome fragment is located downstream from the DNA marker M1-Ct (Hd1) and up to the DNA marker M2-Ag (Hd1) (that is, chromosome 6 of the rice cultivar Koshihikari).
- the region corresponding to the region from the 8,757,819th base to the 8,940,503th base), and the downstream end of the chromosomal fragment is from the DNA marker M4-Gc (Hd1) to the DNA marker. It exists in the region upstream of M5-At (Hd1) (that is, the region corresponding to the region including the 9,533,057th base to the 9,777,195th base).
- FIG. 5 is a diagram schematically showing the genomes of Koshihikari and Koshihikari eichi 3go.
- the heading time of Koshihikari eichi 3 was measured (seeding date: May 6, 2010, transplanting date: June 1, 2010).
- the heading period was from August 5th to August 8th, while Koshihikari Eichi 3 was from July 25th to July 26th.
- Koshihikari Eichi 3 was as early as Koshihikari Eichi 5.
- Koshihikari Eichi 3 was measured in a field in Hokkaido north of 38.5 degrees north latitude (seeding date: April 28, 2010, transplanting date: June 7, 2010). As a result, as shown in FIG. 7, the heading time of Koshihikari was from September 1 to September 2, and rice could not be harvested. In contrast, Koshihikari Eichi No. 3 headed from August 10 to August 16, after which rice could be harvested. In other words, Koshihikari Eichi No. 3 grows significantly faster than Koshihikari Eichi No. 5 when cultivated in a region north of 38.5 degrees north latitude, so it can be cultivated in Hokkaido. It became clear.
- the degree of early-proliferation effect by replacing the Hd1 region with Habataki-derived chromosomal fragments varies depending on the cultivated place, for example, when cultivated in a northern region, for example, a region north of latitude 38.5 degrees north. It is a finding for the first time by the present inventor that the effect of fast-growing is higher than when cultivated in the Koshihikari cultivation area (35.5 to 38.5 degrees north latitude).
- FIG. 8 is a diagram schematically showing the genome of Koshihikari Kazusa No. 6.
- the heading time of Koshihikari Kazusa No. 6 was measured, it was earlier than Koshihikari Eichi No. 3, and even when cultivated in Hokkaido north of 38.5 degrees north latitude, rice could be harvested.
- the expression character of Koshihikari Kazusa No. 6 was compared with Koshihikari, in the actual field test, other characters other than the heading time were almost the same as Koshihikari.
- Koshihikari Kazusa No. 6 is a new variety produced by the method described in Patent Document 2, and has been designed and cultivated such that 99% or more of the genome structure is the same as Koshihikari.
- Koshihikari Kazusa No. 6 is a very good variety that maintains excellent traits such as the taste of Koshihikari despite being so fast that it can be cultivated in Hokkaido, where Koshihikari could not be cultivated. . Therefore, the applicant filed a variety registration application for Koshihikari Kazusa No. 6 as stipulated in the Japanese Seedling Law (Act No. 83 of May 29, 1998). : January 28, 2011, kind registration application number: 25587).
- Rice cultivar Koshihikari Kazusa 6 can be cultivated by the same method as the original cultivar Koshihikari, and rice can be harvested by self-mating or artificial mating.
- the rice variety Koshihikari Kazusa No. 5 and its progeny individuals can be used as parent individuals for breeding new varieties, similar to the original variety Koshihikari. For example, trying to breed a new variety by crossing an individual of the rice variety Koshihikari Kazusa No. 6 with an individual of another variety and backcrossing the obtained progeny individual with an individual of the rice variety Koshihikari Kazusa No. 6 You can also.
- DNA marker M1-Ac QTS14
- DNA marker M2-Ct QTS14
- DNA marker M3-Ag QTS14
- DNA marker M4-Gc QTS14
- Genome information unique to rice cultivar Koshihikari Kazusa No. 6 and rice cultivar Koshihikari Kazuichi 5 Genome information unique to rice cultivar Koshihikari Kazusa No. 6 and rice cultivar Koshihikari Kazuichi 5. Therefore, rice cultivar Koshihikari Kazusa 6 and rice cultivar Koshihikari Kazuichi 5 use these five kinds of DNA markers as appropriate. Can be distinguished.
- genome analysis is performed on rice individuals to be cultivated, and DNA markers M1-Ac (QTS14), DNA markers M2-Ct (QTS14), DNA markers M3-Ag (QTS14), DNA markers
- One or more DNA markers selected from the group consisting of M4-Gc (QTS14) and DNA marker M5-At (QTS14) are typed, and the obtained typing result is consistent with the result of rice cultivar Koshihikari Kazusa No. 6
- the rice individual can be identified as the rice variety Koshihikari kazusa No. 6 or the rice variety Koshihikari eichi No. 5.
- DNA marker M1-Ct (Hd1) DNA marker M2-Ag (Hd1), DNA marker M3-Cg (Hd1), DNA marker M4-Gc (Hd1), and The DNA marker M5-At (Hd1)) is genomic information unique to the rice cultivar Koshihikari Kazusa No. 6 and the rice cultivar Koshihikari eichi 3go. Therefore, the rice cultivar Koshihikari kazusa No. 6 and the rice cultivar Koshihikari eichi 3 can be distinguished using these five types of DNA markers as appropriate.
- genome analysis is carried out on rice individuals whose varieties are to be identified, and DNA markers M1-Ct (Hd1), DNA markers M2-Ag (Hd1), DNA markers M3-Cg (Hd1), DNA markers One or more DNA markers selected from the group consisting of M4-Gc (Hd1) and DNA marker M5-At (Hd1) are typed, and the obtained typing results are consistent with the results of rice cultivar Koshihikari Kazusa No. 6
- the rice individual can be identified as the rice variety Koshihikari kazusa No. 6 or the rice variety Koshihikari eichi No. 3.
- all of the DNA markers M1 to M5 may be used for identifying the varieties, or some of the five DNA markers may be used.
- only DNA markers M1 and M2 that are upstream recombination points may be used, only DNA markers M4 and M5 that are downstream recombination points may be used, or only DNA markers M2 and M4 may be used.
- Good. By appropriately combining a plurality of DNA markers, more rigorous product identification becomes possible.
- the QTS14 region in the third chromosome of rice individuals specifically, the region from at least the DNA marker M2-Ct (QTS14) to the DNA marker M4-Gc (QTS14) (that is, the rice cultivar Nipponbare No.
- the region of rice cultivar Koshihikari kazusa 6 and rice cultivar Koshihikari eichi 5 is composed of a chromosome fragment comprising the region of rice cultivar Habataki, so rice cultivar Koshihikari kazusa 6 or rice cultivar Koshihikari eichi You may substitute by the chromosome fragment which consists of the said area
- a rice individual that is rapidly grown by introducing a chromosome fragment of the region of the rice variety Habataki may be any variety that has the same or similar base sequence as the rice variety Koshihikari. Although it is not limited, it is preferable that it is a rice variety Koshihikari or a new variety produced as a parent variety from the viewpoint of consumer preference.
- the upstream end of the chromosome fragment derived from rice cultivar Habataki (or from rice cultivar Koshihikari Kazusa No. 6 etc.) including the region from DNA marker M2-Ct (QTS14) to DNA marker M4-Gc (QTS14) is the DNA marker M1.
- the Hd1 region in the sixth chromosome specifically, at least the region from the DNA marker M2-Ag (Hd1) to the DNA marker M4-Gc (Hd1) (ie, , The region corresponding to the region from the 8,940,503th base to the 9,533,057th base in the 6th chromosome of the rice cultivar Nipponbare) is replaced with a chromosomal fragment comprising this region of the rice cultivar Habataki By doing so, the rice individual can be remarkably fastened so that it can be cultivated in a region north of 38.5 degrees north latitude.
- the region of rice cultivar Koshihikari kazusa 6 and rice cultivar Koshihikari eichi 3 is composed of a chromosome fragment comprising the region of rice cultivar Habataki, so rice cultivar Koshihikari kazusa 6 or rice cultivar Koshihikari eichi 3 You may substitute by the chromosome fragment which consists of the said area
- a rice individual that is rapidly grown by introducing a chromosome fragment of the region of the rice variety Habataki may be any variety that has the same or similar base sequence as the rice variety Koshihikari. Although it is not limited, it is preferable that it is a rice variety Koshihikari or a new variety produced as a parent variety from the viewpoint of consumer preference.
- the upstream end of the chromosome fragment derived from rice cultivar Habataki (or from rice cultivar Koshihikari Kazusa No. 6 etc.) including the region from DNA marker M2-Ag (Hd1) to DNA marker M4-Gc (Hd1) is the DNA marker M1
- the region downstream of Ct (Hd1) and up to the DNA marker M2-Ag (Hd1) ie, from the 8,757,819th base to the 8,940,503th base in chromosome 6 of the rice cultivar Nipponbare)
- the downstream end of the chromosome fragment extends from the DNA marker M4-Gc (Hd1) to the upstream of the DNA marker M5-At (Hd1) (ie, rice cultivar) It corresponds to the region that includes from the 9,533,057th base to the 9,777,195th base in the 6th chromosome of Nipponbare
- the Hd1 region in chromosome 6, including Koshihikari eichi 3 and Koshihikari kazusa 6 (specifically, at least from DNA marker M2-Ag (Hd1) to DNA marker M4-Gc (Hd1))
- the rice plant whose region is replaced with a chromosome fragment comprising the region of the rice variety Habataki is not only cultivatable in areas where Koshihikari can be cultivated, but also cultivated in areas north of latitude 38.5 degrees north, Rice can be harvested. These rice individuals are affected by temperature, rainfall, and the like, but can be cultivated in an area between 38.5 degrees and 43.3 degrees north latitude, for example.
- the Hd1 gene in the Hd1 region is considered to be a causative gene that causes premature growth.
- a gene contained in the QTS14 region was examined, a region encoding the phytochrome C gene was contained in the region. It has been reported that this gene is mainly involved in the control of plant flowering time (US Pat. No. 7,656,815). Therefore, it is inferred that the causative gene causing prematurity in the QTS14 region is the phytochrome C gene.
- the Hd1 gene is mapped to the region from the 9,335,337th base to the 9,337,606th base of chromosome 6, and the phytochrome C gene is Maps to the region from the 31st, 720, 064th base to the 31st, 724, 043th base of the 3 chromosome.
- the region corresponding to the region from the 31st, 689th, 691st base to the 31st, 724th, 043th base in the 3rd chromosome of the rice variety Nipponbare in the 3rd chromosome of the rice individual It is considered that the rice individual can be born faster than the original cultivar by substituting the cultivar Koshihikari Kazusa No. 6, the rice cultivar Koshihikari eichi No. 5 or the rice cultivar Habataki with the chromosome fragment.
- the upstream end of the chromosome fragment is present in a region corresponding to the region from the 31st, 689th, 690th base to the 31st, 720, 064th base of the third chromosome of the rice cultivar Nipponbare, And the chromosome fragment so that the downstream end of the chromosome fragment exists in a region corresponding to the region from the 31st, 724, 043th base to the 32,298, 685th base of the third chromosome of rice cultivar Nipponbare.
- the region corresponding to the region from the 9,335,337th base to the 9,337,606th base in the 6th chromosome of the rice variety Nipponbare in the 6th chromosome of the rice individual By replacing the Koshihikari kazusa No. 6, the rice cultivar Koshihikari eichi 3 or the rice cultivar Habataki with a chromosomal fragment, it is considered that the rice individual can be born faster than the original cultivar.
- the upstream end of the chromosome fragment is present in a region corresponding to a region including the bases from the 8,940,504th base to the 9,335,337th base of the sixth chromosome of the rice cultivar Nipponbare, And the chromosome fragment so that the downstream end of the chromosome fragment exists in a region corresponding to the region from the 9,337,606th base to the 9,533,056th base of the third chromosome of rice cultivar Nipponbare.
- Koshihikari Eichi No. 5 and Koshihikari Eichi No. 3 were crossed to produce a new variety in which only the QTS14 region and the Hd1 region were replaced with Habataki-derived chromosome fragments in the Koshihikari chromosome. Specifically, Koshihikari eichi 3 and Koshihikari eichi 5 are crossed, 2 of the obtained progeny individuals (seed) are cultivated, self-propagated (self-mating), and seeds that are progeny individuals 100 pieces were obtained.
- Koshihikari Kazusa No. 6 The heading time of Koshihikari Kazusa No. 6 was measured in a field in Chiba Prefecture (seeding date: May 6, 2010, transplanting date: June 1, 2010). The measurement results are shown in FIG. 9 together with the results of Koshihikari, Koshihikari eichi 5 and Koshihikari eichi 3.
- Koshihikari's heading date is from August 5 to August 8, and Koshihikari Eichi 5 and Koshihikari Eichi 3 were from July 24 to July 26, while Koshihikari Kazusa 6 Was from July 18th to July 23rd. From these results, when cultivated in Chiba Prefecture, Koshihikari Kazusa No. 6 was clearly earlier than Koshihikari Eichi No. 3 and Koshihikari Echigo No. 5.
- Koshihikari Kazusa No. 6 was measured in a farm field in Hokkaido (43.3 degrees north latitude) (seeding date: April 28, 2010, transplanting date: June 7, 2010). The measurement results are shown in FIG. 10 together with the results of Koshihikari, Koshihikari eichi 5 and Koshihikari eichi 3.
- the heading season for Koshihikari is from September 1 to September 2
- Koshihikari Eichi No. 5 is from August 21 to August 22
- Koshihikari Eichi No. 3 is from August 10 to August 16.
- Koshihikari Kazusa No. 6 was from August 7th to August 9th.
- Koshihikari Kazusa No. 6 can be cultivated in Hokkaido north of 38.5 degrees north latitude.
- Koshihikari Kazusa No. 6 and Koshihikari were compared (implemented in Chiba Prefecture in 2009).
- the examination of the traits was conducted in accordance with the characteristic examination for the variety registration application based on Article 5 Paragraph 1 of the Seedling and Seedling Law (1998 Law 83).
- the examination results are shown in Tables 3-6.
- Koshihikari Kazusa No. 6 was about two weeks ahead of Koshihikari in both heading and maturity.
- Koshihikari Kazusa No.6 was slightly shorter than the Koshihikari, but the stem length and main stem length and main stem length were slightly shorter, and the number of ears and the number of main stem grains were smaller, but other traits were basically Koshihikari. Was the same.
- the rice cultivar Koshihikari Kazusa No. 6 which is a new variety of the present invention has almost the same characteristics as Koshihikari, and can be cultivated in the northern region more than before, so it can be used particularly in the field of agriculture. Moreover, since the rice individual of the present invention can be made to grow faster than the original variety, the method can be used particularly in the field of plant breeding.
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Abstract
Description
(1) 品種登録出願番号が第25587号である、イネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)、
(2) 前記(1)記載の品種の個体及び前記(1)記載の品種の個体の後代個体からなる群より選択される2個体を交配して得られる後代個体、
(3) あるイネ個体が、特定の品種であるか否かを鑑別する方法であって、
イネ品種日本晴の第3染色体中の31,521,442番目のSNP(一塩基多型)に相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではC)をDNAマーカーM1とし、
イネ品種日本晴の第3染色体の31,689,690番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではT)をDNAマーカーM2とし、
イネ品種日本晴の第3染色体の32,208,924番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではG)をDNAマーカーM3とし、
イネ品種日本晴の第3染色体の32,298,686番目のSNPに相当するSNP(イネ品種コシヒカリではG、イネ品種ハバタキではC)をDNAマーカーM4とし、
イネ品種日本晴の第3染色体の32,363,157番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではT)をDNAマーカーM5とし、
当該イネ個体のゲノム解析により、前記DNAマーカーM1~M5からなる群より選択される1以上のDNAマーカーをタイピングし、
得られたタイピング結果がイネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)又はイネ品種コシヒカリえいち5号(Oryza sativa L.cultivar Koshihikari-eich5 gou)の結果と一致する場合に、当該イネ個体がイネ品種コシヒカリかずさ6号又はイネ品種コシヒカリえいち5号であると鑑別することを特徴とする、イネ品種の鑑別方法、
(4) あるイネ個体が、特定の品種であるか否かを鑑別する方法であって、
イネ品種日本晴の第6染色体の8,757,818番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではT)をDNAマーカーM1とし、
イネ品種日本晴の第6染色体の8,940,503番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではG)をDNAマーカーM2とし、
イネ品種日本晴の第6染色体の9,325,062番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではG)をDNAマーカーM3とし、
イネ品種日本晴の第6染色体の9,533,057番目のSNPに相当するSNP(イネ品種コシヒカリではG、イネ品種ハバタキではC)をDNAマーカーM4とし、
イネ品種日本晴の第6染色体の9,777,196番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではT)をDNAマーカーM5とし、
当該イネ個体のゲノム解析により、前記DNAマーカーM1~M5からなる群より選択される1以上のDNAマーカーをタイピングし、
得られたタイピング結果がイネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)又はイネ品種コシヒカリえいち3号(Oryza sativa L.cultivar Koshihikari-eich3 gou)の結果と一致する場合に、当該イネ個体がイネ品種コシヒカリかずさ6号又はイネ品種コシヒカリえいち3号であると鑑別することを特徴とする、イネ品種の鑑別方法、
(5) イネ個体の第3染色体中の、イネ品種日本晴の第3染色体中の31,720,064番目の塩基から31,724,043番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号又はイネ品種ハバタキの当該領域からなる染色体断片に置換することを特徴とする、イネ個体を早生化する方法、
(6) 前記染色体断片の上流端が、イネ品種日本晴の第3染色体の31,689,691番目の塩基から31,720,064番目の塩基までを含む領域に相当する領域に存在し、かつ当該染色体断片の下流端が、イネ品種日本晴の第3染色体の31,724,043番目の塩基から32,298,685番目の塩基までを含む領域に相当する領域に存在するように、当該染色体断片を置換することを特徴とする前記(5)記載のイネ個体を早生化する方法、
(7) イネ個体の第3染色体中の、イネ品種日本晴の第3染色体中の31,689,690番目の塩基から32,298,686番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号又はイネ品種ハバタキの当該領域からなる染色体断片に置換することを特徴とする、イネ個体を早生化する方法、
(8) 前記染色体断片の上流端が、上流端がイネ品種日本晴の第3染色体の31,521,443番目の塩基から31,689,690番目の塩基までを含む領域に相当する領域に存在し、かつ当該染色体断片の下流端が、イネ品種日本晴の第3染色体の32,298,686番目の塩基から32,363,156番目の塩基までを含む領域に相当する領域に存在するように、当該染色体断片を置換することを特徴とする前記(7)記載のイネ個体を早生化する方法、
(9) 前記(5)~(8)のいずれか一つに記載のイネ個体を早生化する方法により作出されたイネ品種、
(10) 前記(9)記載の品種の個体及び前記(9)記載の品種の個体の後代個体からなる群より選択される2個体を交配して得られる後代個体、
(11) イネ個体の第6染色体中の、イネ品種日本晴の第6染色体中の8,940,503番目の塩基から9,533,057番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号、イネ品種コシヒカリえいち3号、又はイネ品種ハバタキの当該領域からなる染色体断片に置換されたイネ個体、イネ品種コシヒカリかずさ6号のイネ個体、及びイネ品種コシヒカリえいち3号のイネ個体からなる群より選択される1種以上のイネ個体を、北緯38.5度よりも北で栽培することを特徴とするイネの栽培方法、を、提供するものである。
また、本発明のイネ品種の鑑別方法により、イネ品種コシヒカリかずさ6号を鑑別することができる。
また、本発明のイネ個体を早生化する方法により、イネ個体を元品種よりも早生化することができる
コシヒカリえいち5号とコシヒカリえいち3号とを掛け合わせ、コシヒカリの染色体中、QTS14領域とHd1領域のみがハバタキ由来の染色体断片に置換された新品種を作製した。
具体的には、コシヒカリえいち3号とコシヒカリえいち5号を交配し、得られた後代個体(種子)のうち2個を栽培し、自殖(自家交配)させ、さらに後代個体である種子を100個得た。この100個の種子を全て栽培し、各後代個体のDNAマーカーを調べ、DNAマーカーM3-Cg(Hd1)とDNAマーカーM3-Ag(QTS14)の両方がハバタキ由来アレルのホモ染色体領域である栽培個体を1個体選抜した。本発明者はこの新品種を「コシヒカリかずさ5号」と命名した。
Claims (11)
- 品種登録出願番号が第25587号である、イネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)。
- 請求項1記載の品種の個体及び請求項1記載の品種の個体の後代個体からなる群より選択される2個体を交配して得られる後代個体。
- あるイネ個体が、特定の品種であるか否かを鑑別する方法であって、
イネ品種日本晴の第3染色体中の31,521,442番目のSNP(一塩基多型)に相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではC)をDNAマーカーM1とし、
イネ品種日本晴の第3染色体の31,689,690番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではT)をDNAマーカーM2とし、
イネ品種日本晴の第3染色体の32,208,924番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではG)をDNAマーカーM3とし、
イネ品種日本晴の第3染色体の32,298,686番目のSNPに相当するSNP(イネ品種コシヒカリではG、イネ品種ハバタキではC)をDNAマーカーM4とし、
イネ品種日本晴の第3染色体の32,363,157番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではT)をDNAマーカーM5とし、
当該イネ個体のゲノム解析により、前記DNAマーカーM1~M5からなる群より選択される1以上のDNAマーカーをタイピングし、
得られたタイピング結果がイネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)又はイネ品種コシヒカリえいち5号(Oryza sativa L.cultivar Koshihikari-eich5 gou)の結果と一致する場合に、当該イネ個体がイネ品種コシヒカリかずさ6号又はイネ品種コシヒカリえいち5号であると鑑別することを特徴とする、イネ品種の鑑別方法。 - あるイネ個体が、特定の品種であるか否かを鑑別する方法であって、
イネ品種日本晴の第6染色体の8,757,818番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではT)をDNAマーカーM1とし、
イネ品種日本晴の第6染色体の8,940,503番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではG)をDNAマーカーM2とし、
イネ品種日本晴の第6染色体の9,325,062番目のSNPに相当するSNP(イネ品種コシヒカリではC、イネ品種ハバタキではG)をDNAマーカーM3とし、
イネ品種日本晴の第6染色体の9,533,057番目のSNPに相当するSNP(イネ品種コシヒカリではG、イネ品種ハバタキではC)をDNAマーカーM4とし、
イネ品種日本晴の第6染色体の9,777,196番目のSNPに相当するSNP(イネ品種コシヒカリではA、イネ品種ハバタキではT)をDNAマーカーM5とし、
当該イネ個体のゲノム解析により、前記DNAマーカーM1~M5からなる群より選択される1以上のDNAマーカーをタイピングし、
得られたタイピング結果がイネ品種コシヒカリかずさ6号(Oryza sativa L.cultivar Koshihikari-kazusa6 gou)又はイネ品種コシヒカリえいち3号(Oryza sativa L.cultivar Koshihikari-eich3 gou)の結果と一致する場合に、当該イネ個体がイネ品種コシヒカリかずさ6号又はイネ品種コシヒカリえいち3号であると鑑別することを特徴とする、イネ品種の鑑別方法。 - イネ個体の第3染色体中の、イネ品種日本晴の第3染色体中の31,720,064番目の塩基から31,724,043番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号又はイネ品種ハバタキの当該領域からなる染色体断片に置換することを特徴とする、イネ個体を早生化する方法。
- 前記染色体断片の上流端が、イネ品種日本晴の第3染色体の31,689,691番目の塩基から31,720,064番目の塩基までを含む領域に相当する領域に存在し、かつ当該染色体断片の下流端が、イネ品種日本晴の第3染色体の31,724,043番目の塩基から32,298,685番目の塩基までを含む領域に相当する領域に存在するように、当該染色体断片を置換することを特徴とする請求項5記載のイネ個体を早生化する方法。
- イネ個体の第3染色体中の、イネ品種日本晴の第3染色体中の31,689,690番目の塩基から32,298,686番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号又はイネ品種ハバタキの当該領域からなる染色体断片に置換することを特徴とする、イネ個体を早生化する方法。
- 前記染色体断片の上流端が、上流端がイネ品種日本晴の第3染色体の31,521,443番目の塩基から31,689,690番目の塩基までを含む領域に相当する領域に存在し、かつ当該染色体断片の下流端が、イネ品種日本晴の第3染色体の32,298,686番目の塩基から32,363,156番目の塩基までを含む領域に相当する領域に存在するように、当該染色体断片を置換することを特徴とする請求項7記載のイネ個体を早生化する方法。
- 請求項5~8のいずれか一項に記載のイネ個体を早生化する方法により作出されたイネ品種。
- 請求項9記載の品種の個体及び請求項9記載の品種の個体の後代個体からなる群より選択される2個体を交配して得られる後代個体。
- イネ個体の第6染色体中の、イネ品種日本晴の第6染色体中の8,940,503番目の塩基から9,533,057番目の塩基までを含む領域に相当する領域を、イネ品種コシヒカリかずさ6号、イネ品種コシヒカリえいち3号、又はイネ品種ハバタキの当該領域からなる染色体断片に置換されたイネ個体、イネ品種コシヒカリかずさ6号のイネ個体、及びイネ品種コシヒカリえいち3号のイネ個体からなる群より選択される1種以上のイネ個体を、北緯38.5度よりも北で栽培することを特徴とするイネの栽培方法。
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CN201180069335.3A CN103429073B (zh) | 2011-03-18 | 2011-03-18 | 新品种的培育方法、植物品种的鉴别方法以及使水稻个体早熟的方法 |
US14/005,225 US9029669B2 (en) | 2011-03-18 | 2011-03-18 | Cultivar, method for differentiating plant cultivars, and method for causing earlier maturing of rice individual |
JP2011513769A JP4892647B1 (ja) | 2011-03-18 | 2011-03-18 | 新品種、植物品種の鑑別方法、及びイネ個体を早生化する方法 |
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CN104805193A (zh) * | 2015-03-31 | 2015-07-29 | 江汉大学 | 一种测试水稻品种实质性派生关系的方法 |
CN110028567A (zh) * | 2019-04-22 | 2019-07-19 | 江西农业大学 | 一种水稻开花相关的蛋白质及其编码基因lhd3与应用 |
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WO2004044200A1 (ja) * | 2002-11-13 | 2004-05-27 | Honda Motor Co., Ltd. | 穀物の収量を増加させる遺伝子、並びにその利用 |
JP2008283902A (ja) * | 2007-05-17 | 2008-11-27 | Honda Motor Co Ltd | イネおよびその作出方法 |
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JP2008283902A (ja) * | 2007-05-17 | 2008-11-27 | Honda Motor Co Ltd | イネおよびその作出方法 |
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JPWO2012127559A1 (ja) | 2014-07-24 |
CN103429073A (zh) | 2013-12-04 |
JP4892647B1 (ja) | 2012-03-07 |
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