WO2012118365A1 - A method of producing a bacterial inoculum using the rumen of a ruminant animal - Google Patents

A method of producing a bacterial inoculum using the rumen of a ruminant animal Download PDF

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Publication number
WO2012118365A1
WO2012118365A1 PCT/MY2012/000043 MY2012000043W WO2012118365A1 WO 2012118365 A1 WO2012118365 A1 WO 2012118365A1 MY 2012000043 W MY2012000043 W MY 2012000043W WO 2012118365 A1 WO2012118365 A1 WO 2012118365A1
Authority
WO
WIPO (PCT)
Prior art keywords
rumen
mixture
producing
bacterial inoculum
filtrate
Prior art date
Application number
PCT/MY2012/000043
Other languages
French (fr)
Inventor
Mustafa Al Bakri Abdullah MOHD
Hussin KAMARUDIN
Mohd Ruzaidi Ghazali CHE
Hadi Ma'Radzi AKMAL
Mohamed Noor NORAZIAN
Zainul Kamal SYAZNI
Sabran SALEHUDDIN
Jaafar Sahari MOHAMAD
Hafiz Ab Halim MOHD
Sani Timan SHAM
Suaimi Ahmad TAUFIK
Original Assignee
Universiti Malaysia Perlis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Malaysia Perlis filed Critical Universiti Malaysia Perlis
Publication of WO2012118365A1 publication Critical patent/WO2012118365A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • This present invention relates to a method of producing a bacterial inoculum.
  • the present invention relates to a method of producing a bacterial inoculum using a culture medium by fermentation.
  • the increase in agricultural activities can produce wastes and cause pollutions to the environment through the excessive use of chemical fertilizers, herbicides and pesticides.
  • a United States Patent No. 2007039362 discloses a progressive digestion process for producing fertilizer.
  • a mixture of organic inputs including digestive enzyme source inputs, pH adjustment inputs and nutrient sources is used as the starting materials.
  • Mesophilic and thermophilic digestion are performed on the mixture to produce a fertilizer product of a liquid form. Since the fermentation is carried out in several stages, the pH and temperature have to be monitored within different suitable range for each stage.
  • a method of manufacturing a fertilizer made from organic wastes is- described in another United State Patent No. 6273927. In the prior art, organic wastes are reacted with a mineral material such as calcined lime.
  • a Japanese Patent No. 2000026181 describes an organic fertilizer and its production thereof.
  • the process comprising the steps of forming an active material, obtaining a microbial material using the resulting active material, adding various kinds of yeast and if necessary a culture adjustment agent to the microbial material, fermenting the mixture to form a liquid material, mixing the liquid material with feces to form a mixture in a full-ripe state, adding rice hulls then separating the rice hull fraction from the resulting mixture, removing water from the separated rice hull fraction and subjecting the fraction to exothermic fermentation and ripening to produce the objective product.
  • 2006290690 describes a compost for agriculture using seafood wastes as a raw material where useful microorganisms containing lactobacillus, mold and photosynthetic bacterium are easily proliferated and where effective fermentation is prompted.
  • the seafood wastes, useful microorganism and a humidifying material are mixed and stirred to be fermented for a specified period in a temperature-controlled fermenter.
  • the prior arts mainly claim the production of fertilizer from organic or waste materials and the fermentation of materials is usually performed by using commercial externally added microorganisms thus increasing the production cost.
  • the present invention features a bacterial inoculum which is cultured from organic or waste materials and further processed into other beneficial products including • fertilizer, waste water treatment agent, pest control and waste composting agent.
  • the main object of the present invention is to invent a method of producing a bacterial inoculum using organic or waste materials as a growth culture medium for optimizing microbial proliferation.
  • One object of the present invention is to invent a method of producing a bacterial inoculum through fermentation of organic or waste materials using yeast to proliferate the microorganisms which are readily available from the materials.
  • the production of bacterial inoculum is cost efficient without using commercial externally added microorganisms.
  • Another object of the present invention is to invent a method of producing a bacterial inoculum which is a Halal product suitably to be used by Muslim consumers and could be in the form of powder or granule for easy packaging and storage.
  • the embodiment of the present invention describes a method of producing a bacterial inoculum comprising the steps of fermenting a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen, extracting a filtrate from the fermented mixture, subjecting the filtrate to a drying process and forming the dried product into powder or granules.
  • the present invention describes a method of producing a bacterial inoculum from a mixture consisting of the rumen of ruminant animals, food energy sources and water to be used as a culture medium fermented by yeast.
  • the production method is cost efficient as it is not required to externally add in commercial microorganisms during the fermentation, instead the microorganisms can be readily available as obtained from the rumen itself.
  • the culture medium could provide sufficient energy and nutrients for optimizing microbial proliferation.
  • the present invention discloses a method of producing a bacterial inoculum comprising the steps of fermenting a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen, extracting a filtrate from the fermented mixture, subjecting the filtrate to a drying process and forming the dried product into powder or granules.
  • the method of producing a bacterial inoculum is by fermenting the mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast wherein the mixture could function as a culture medium for bacterial proliferation.
  • the yeast can actively produce various biologically active ⁇ agents such as amino acids and polysaccharides to initiate the fermentation process so : that a good bacterial inoculum could be produced.
  • microorganisms used during fermentation are readily available from the rumen itself and therefore does not required the use of commercial externally added microorganisms. This could in fact reduce the production cost.
  • the mixture consisting of the rumen of ruminant animals, food energy sources and water is a culture medium which provides energy and nutrients for optimizing the bacterial proliferation.
  • the food energy sources shall include agricultural wastes, aquaculture wastes, milk, molasses and animal feed bran.
  • the agricultural wastes could be tropical fruits such as papaya, banana and others that are rich in sugar content whereas the aquaculture wastes are fish and shrimp wastes.
  • One preferred embodiment of the present invention claims a method of producing a bacterial inoculum as a Halal product suitably and safe to be used by Muslim consumers.
  • the rumen is preferably obtained from Halal slaughtered animals.
  • Another preferred embodiment describes the . method of producing a bacterial inoculum comprising the step of fermenting the mixture preferably consisting of rumen in an amount ranging from 30wt% to 70wt% while energy sources in an amount ranging from 30wt% to 70wt% of the mixture.
  • Yeast is used in an amount ranging from 0.2wt% to 10wt% of the mixture.
  • the present invention claims a method of producing a bacterial inoculum comprising the step of extracting a filtrate from the fermented mixture.
  • the filtrate is non- toxic, environmental friendly with no side effects to plants, animals and human and is the successor of pesticides and chemical fertilizers. ⁇ ;
  • the extracted filtrate in a liquid, slurry or paste form contains a high amount of microorganisms could be used as a natural biological agent for repacking various agricultural wastes into useful materials such as compost, bio-gas and biomass.
  • the filtrate could be used to be further processed into other beneficial products such as fertilizer, waste water treatment agent, pest control and waste composting agent.
  • the filtrate is diluted with water and mixed with agrowastes including dried leaves, cow litter, rotten wood and others to produce a fertilizer.
  • the present invention also describes the production of bacterial inoculum in the form of powder or granules for easy packaging and storage.
  • the filtrate is subjected to a drying process preferably at a low temperature spray drying to form into powder or granules.
  • a preferred embodiment of the present invention discloses that the production of bacterial inoculum in the form of powder or granules is preferably performed by feeding the liquid, slurry or paste form of the filtrate containing the cultured microorganisms to a spray drying chamber through a spraying nozzle.
  • a dehumidifier is attached to the chamber for speeding up the drying process by absorbing water or moisture from the spraying droplets.
  • the preferred temperature for carrying out the spray drying process is below 100°C so that the microorganisms are not destroyed at a higher temperature.
  • the most preferred temperature for the drying process is ranging from 40°C to 90°C.
  • the powder or granular form of the bacterial inoculum could be produced in a range of 50-3 ⁇ depending on the pressure and size of the nozzle.

Abstract

A method of producing a bacterial inoculum comprising the steps of fermenting a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen, extracting a filtrate from the fermented mixture, subjecting the filtrate to a drying process and forming the dried product into powder or granules.

Description

A METHOD OF PRODUCING A BACTERIAL INOCULUM USING
THE RUMEN OF A RUMINANT ANIMAL
Field of Invention
This present invention relates to a method of producing a bacterial inoculum. In more particular, the present invention relates to a method of producing a bacterial inoculum using a culture medium by fermentation. Background of The Invention
The increase in agricultural activities can produce wastes and cause pollutions to the environment through the excessive use of chemical fertilizers, herbicides and pesticides.
Hence, noble efforts have been taken to utilize natural biological agents such as microorganisms to repackage the agricultural wastes into useful materials such as compost, bio-gas and biomass to protect the environment and reduce production costs of farming industry.
There are some prior arts relating to several methods of processing organic wastes into useful product, as such a fertilizer.
A United States Patent No. 2007039362 discloses a progressive digestion process for producing fertilizer. A mixture of organic inputs including digestive enzyme source inputs, pH adjustment inputs and nutrient sources is used as the starting materials. Mesophilic and thermophilic digestion are performed on the mixture to produce a fertilizer product of a liquid form. Since the fermentation is carried out in several stages, the pH and temperature have to be monitored within different suitable range for each stage. A method of manufacturing a fertilizer made from organic wastes is- described in another United State Patent No. 6273927. In the prior art, organic wastes are reacted with a mineral material such as calcined lime.' or a dolomite as a natural digestant to neutralize the toxicity and remove the odor with sterilizing therein so as to give the fertilizer source. The fertilizer source could then be added with the special supplement material for secondary processing into a compost fertilizer, an organic fertilizer for special crops or an organic fertilizer used as barnyard manure. A Japanese Patent No. 2000026181 describes an organic fertilizer and its production thereof. The process comprising the steps of forming an active material, obtaining a microbial material using the resulting active material, adding various kinds of yeast and if necessary a culture adjustment agent to the microbial material, fermenting the mixture to form a liquid material, mixing the liquid material with feces to form a mixture in a full-ripe state, adding rice hulls then separating the rice hull fraction from the resulting mixture, removing water from the separated rice hull fraction and subjecting the fraction to exothermic fermentation and ripening to produce the objective product. Still, another Japanese Patent No. 2006290690 describes a compost for agriculture using seafood wastes as a raw material where useful microorganisms containing lactobacillus, mold and photosynthetic bacterium are easily proliferated and where effective fermentation is prompted. The seafood wastes, useful microorganism and a humidifying material are mixed and stirred to be fermented for a specified period in a temperature-controlled fermenter.
The prior arts mainly claim the production of fertilizer from organic or waste materials and the fermentation of materials is usually performed by using commercial externally added microorganisms thus increasing the production cost. However, the present invention features a bacterial inoculum which is cultured from organic or waste materials and further processed into other beneficial products including • fertilizer, waste water treatment agent, pest control and waste composting agent.
It is desirable to invent a method of producing a bacterial inoculum using a growth culture medium for establishing optimized microbial proliferation. It is also desirable to invent a method of producing a bacterial inoculum through fermentation of organic or waste materials using yeast to proliferate the microorganisms which are readily available from the materials. Besides, it is advantageous to produce a ba'cterial inoculum in powder or granular form for easy packaging and storage.
Summary of The Invention
The main object of the present invention is to invent a method of producing a bacterial inoculum using organic or waste materials as a growth culture medium for optimizing microbial proliferation.
One object of the present invention is to invent a method of producing a bacterial inoculum through fermentation of organic or waste materials using yeast to proliferate the microorganisms which are readily available from the materials. Thus, the production of bacterial inoculum is cost efficient without using commercial externally added microorganisms.
Another object of the present invention is to invent a method of producing a bacterial inoculum which is a Halal product suitably to be used by Muslim consumers and could be in the form of powder or granule for easy packaging and storage.
At least one of the preceding objects is met, in whole or in part, by the present invention, in which the embodiment of the present invention describes a method of producing a bacterial inoculum comprising the steps of fermenting a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen, extracting a filtrate from the fermented mixture, subjecting the filtrate to a drying process and forming the dried product into powder or granules.
The present invention describes a method of producing a bacterial inoculum from a mixture consisting of the rumen of ruminant animals, food energy sources and water to be used as a culture medium fermented by yeast.
The production method is cost efficient as it is not required to externally add in commercial microorganisms during the fermentation, instead the microorganisms can be readily available as obtained from the rumen itself.
In the present invention, the culture medium could provide sufficient energy and nutrients for optimizing microbial proliferation.
Detailed Description of The Invention
One skilled in the art will readily appreciate that the present invention is well adapted to carry out the objects and obtain the ends and advantages mentioned, as well as those inherent therein. The embodiment described herein is not intended as limitations on the scope of the invention.
The present invention discloses a method of producing a bacterial inoculum comprising the steps of fermenting a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen, extracting a filtrate from the fermented mixture, subjecting the filtrate to a drying process and forming the dried product into powder or granules.
In the present invention, the method of producing a bacterial inoculum is by fermenting the mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast wherein the mixture could function as a culture medium for bacterial proliferation. The yeast can actively produce various biologically active ■ agents such as amino acids and polysaccharides to initiate the fermentation process so: that a good bacterial inoculum could be produced.
It is advantageous that the microorganisms used during fermentation are readily available from the rumen itself and therefore does not required the use of commercial externally added microorganisms. This could in fact reduce the production cost.
As further disclosed in the present invention, the mixture consisting of the rumen of ruminant animals, food energy sources and water is a culture medium which provides energy and nutrients for optimizing the bacterial proliferation. The food energy sources shall include agricultural wastes, aquaculture wastes, milk, molasses and animal feed bran.
In particular, the agricultural wastes could be tropical fruits such as papaya, banana and others that are rich in sugar content whereas the aquaculture wastes are fish and shrimp wastes.
One preferred embodiment of the present invention claims a method of producing a bacterial inoculum as a Halal product suitably and safe to be used by Muslim consumers. In a matter of fact, the rumen is preferably obtained from Halal slaughtered animals.
Another preferred embodiment describes the . method of producing a bacterial inoculum comprising the step of fermenting the mixture preferably consisting of rumen in an amount ranging from 30wt% to 70wt% while energy sources in an amount ranging from 30wt% to 70wt% of the mixture. Yeast is used in an amount ranging from 0.2wt% to 10wt% of the mixture. The fermentation step using yeast is preferably performed at a temperature ranging from 15°C to 35°C but most preferably at room temperature which is between 25°C and 28 °C. =.. .
Hereinafter,, the present invention claims a method of producing a bacterial inoculum comprising the step of extracting a filtrate from the fermented mixture. The filtrate is non- toxic, environmental friendly with no side effects to plants, animals and human and is the successor of pesticides and chemical fertilizers. · ;
The extracted filtrate in a liquid, slurry or paste form contains a high amount of microorganisms could be used as a natural biological agent for repacking various agricultural wastes into useful materials such as compost, bio-gas and biomass.
In more particular, the filtrate could be used to be further processed into other beneficial products such as fertilizer, waste water treatment agent, pest control and waste composting agent. In an example, the filtrate is diluted with water and mixed with agrowastes including dried leaves, cow litter, rotten wood and others to produce a fertilizer.
The present invention also describes the production of bacterial inoculum in the form of powder or granules for easy packaging and storage. The filtrate is subjected to a drying process preferably at a low temperature spray drying to form into powder or granules.
A preferred embodiment of the present invention discloses that the production of bacterial inoculum in the form of powder or granules is preferably performed by feeding the liquid, slurry or paste form of the filtrate containing the cultured microorganisms to a spray drying chamber through a spraying nozzle. A dehumidifier is attached to the chamber for speeding up the drying process by absorbing water or moisture from the spraying droplets.
The preferred temperature for carrying out the spray drying process is below 100°C so that the microorganisms are not destroyed at a higher temperature. However, the most preferred temperature for the drying process is ranging from 40°C to 90°C.
In the present invention, the powder or granular form of the bacterial inoculum could be produced in a range of 50-3 ΟΟμιτι depending on the pressure and size of the nozzle.

Claims

Claims
1 . A method of producing a bacterial inoculum comprising the steps of:
a) fermenting, a mixture consisting of the rumen of ruminant animals, food energy sources and water using yeast to proliferate the readily available microorganisms obtained from the rumen;
b) extracting a filtrate from the fermented mixture;
c) subjecting the filtrate to a drying process; and
d) forming the dried product into powder or granules. ' ·
2. A method as claimed in claim 1, wherein the rumen is obtained from Halal slaughtered animals.
3. A method as claimed in claim 1 or claim 2, wherein the food energy sources are agricultural wastes, aquaculture wastes, milk, molasses and animal feed bran.
4. A method as claimed in claim 1, wherein the rumen is used in an amount ranging from 30wt% to 70 t% of the mixture.
5. A method as claimed in claim 1 or claim 3, wherein the energy sources are used in an amount ranging from 30wt% to 70wt% of the mixture.
6. A method as claimed in claim 1, wherein the yeast is used in an amount ranging from 0.2wt% to 10wt% of the mixture.
7. A method as claimed in any of claims 1 to 6, wherein the fermenting step is carried out at a temperature ranging from 15°C to_35°C.
8. A method as claimed in any of claims 1 to 7, wherein the drying process is spray drying at a temperature ranging from 40°C to 90°C using a dehumidifier.
PCT/MY2012/000043 2011-03-03 2012-03-05 A method of producing a bacterial inoculum using the rumen of a ruminant animal WO2012118365A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2011000970A MY157258A (en) 2011-03-03 2011-03-03 A method of producing a bacterial inoculum
MYPI2011000970 2011-03-03

Publications (1)

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WO2012118365A1 true WO2012118365A1 (en) 2012-09-07

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015052369A1 (en) 2013-10-08 2015-04-16 Eino Elias Hakalehto Utilization of intestinal bacteria from slaughtered animals in industrial processes and waste treatment

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3563758A (en) * 1968-02-12 1971-02-16 Con Ferm Inc Animal feed of rumen contents with malt diastase,wood charcoal,and protopeptone
US5459053A (en) * 1995-01-25 1995-10-17 The United States Of America As Represented By The Secretary Of Agriculture Use of rumen contents from slaughter cattle for the production of lactic acid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3563758A (en) * 1968-02-12 1971-02-16 Con Ferm Inc Animal feed of rumen contents with malt diastase,wood charcoal,and protopeptone
US5459053A (en) * 1995-01-25 1995-10-17 The United States Of America As Represented By The Secretary Of Agriculture Use of rumen contents from slaughter cattle for the production of lactic acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015052369A1 (en) 2013-10-08 2015-04-16 Eino Elias Hakalehto Utilization of intestinal bacteria from slaughtered animals in industrial processes and waste treatment
AU2014333722B2 (en) * 2013-10-08 2018-06-28 Eino Elias Hakalehto Utilization of intestinal bacteria from slaughtered animals in industrial processes and waste treatment

Also Published As

Publication number Publication date
MY157258A (en) 2016-05-31

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