WO2012093804A2 - Composition stimulant la croissance des cheveux contenant des saponines rd et re à titre de principes actifs - Google Patents

Composition stimulant la croissance des cheveux contenant des saponines rd et re à titre de principes actifs Download PDF

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Publication number
WO2012093804A2
WO2012093804A2 PCT/KR2011/010274 KR2011010274W WO2012093804A2 WO 2012093804 A2 WO2012093804 A2 WO 2012093804A2 KR 2011010274 W KR2011010274 W KR 2011010274W WO 2012093804 A2 WO2012093804 A2 WO 2012093804A2
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WO
WIPO (PCT)
Prior art keywords
hair growth
hair
saponin
composition
growth
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Application number
PCT/KR2011/010274
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English (en)
Korean (ko)
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WO2012093804A3 (fr
Inventor
고리주안
이청하
이정
이진재
장동량
모은경
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(주)대덕바이오
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Publication of WO2012093804A2 publication Critical patent/WO2012093804A2/fr
Publication of WO2012093804A3 publication Critical patent/WO2012093804A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • the present invention relates to a composition for hair growth, and more particularly, to a hair growth composition which is excellent in inhibiting hair loss and promoting hair growth but not toxic to the human body.
  • Hair growth and dropping cycles are divided into three phases: the growth phase, which is the stage at which the hair grows, the degeneration phase, which leads to the disappearance of the hair, the resting phase, which is the stage of growth stoppage, and the generator, the stage at which new hair develops and grows. Repeated over ⁇ 6 years. As a result, 60-100 hairs are lost on an average day, but when the number of hairs in the developing and growing phases decreases, and the ratio of the degenerative and resting periods increases, the hairs fall abnormally and the hair loss progresses.
  • Hair loss although not yet accurately identified, is known to be caused by a variety of factors, including genetic factors, hormonal imbalances, excessive stress, seborrheic dermatitis, poor circulation of the scalp, and malnutrition.
  • factors including genetic factors, hormonal imbalances, excessive stress, seborrheic dermatitis, poor circulation of the scalp, and malnutrition.
  • the population suffering from hair loss due to excessive increase in stress due to changes in the social environment has been increasing, and age groups and gender restrictions and boundaries are also widening.
  • alopecia such as wigs
  • surgical procedures such as transplant surgery, or drug therapy.
  • Surgical procedures are excellent in terms of effectiveness, but the costs are enormous and the surgical process is difficult, and the economic and mental burden of the patient is very high.
  • Minoxidil which is widely used in drug therapy, was originally used as an antihypertensive agent. However, as a side effect was reported, hair growth began to be used as a hair growth promoter. Minoxidil is applied to androgenetic alopecia, and its effect is verified for head alopecia, but it is ineffective for temporal alopecia. In addition, there is a possibility of causing side effects such as deprivation of antihypertensive action, hypotension, tachycardia, cardiovascular complications are not suitable for long-term use. Propecia, which inhibits the production, binding, and metabolism of androgen, a male hormone, can cause feminization of men, and women of childbearing age have a high risk of birth defects. As such, drug therapy using chemicals causes an unbalance of homeostasis in the body, and various unexpected side effects occur, and as an alternative, research on hair regrowth using natural materials having no side effects is being actively conducted.
  • Ginseng is harmless to the human body even after taking it for a long time. It is known that ginseng has various effects such as fatigue recovery, anti-aging, tonic, and anticancer as a drug that can control the state of the body and prevent aging. . Ginseng contains about 30 kinds of saponins which show inherent bioactivity. In addition, there is a difference in the total saponin content according to the type, processing method, and site of ginseng, as well as the distribution of the saponin type. Saponins are a compound called glycosides and are known to exist in various plants such as ginseng, bellflower, licorice, soybeans and aloe as well as in animals such as sea cucumbers and starfish. Recently, a lot of research has been conducted on the mass production of certain components of saponins.
  • the composition for hair growth can be prepared.
  • the present invention for achieving the above object relates to a composition for hair growth comprising a saponin Rd or Re as an active ingredient.
  • the present inventors have thoroughly investigated the hair growth properties of the saponins Rg2, Rg1, Re, Rh1, Rg3, Rb1, Rd and the minoxidil according to the prior art, and the present invention has been completed.
  • saponin Rd has an effect of extending the growth period of the hair, the growth rate of the entire experimental period is 33.2%, showing an effect of about 1.5 times increase compared to the minoxidil of 22.1% It was.
  • Other saponins had similar effects to minoxidil on hair growth phase.
  • Saponin Re showed a significant effect on minoxidil and other saponins in the hair growth area, and hair growth was observed in a 2.8-fold wider area than minoxidil.
  • the composition of the present invention is based on the efficacy of the saponin Rd and Re as described above, characterized in that it contains Rd or Re. In addition, the composition of the present invention may contain Rd and Re at the same time.
  • composition of the present invention may be prepared by methods known in the pharmaceutical and food science fields for use as a therapeutic agent and food, and may be a carrier or an excipient (pharmaceutically) or a pharmaceutically or food acceptable solvent. agent), diluent and the like can be used.
  • the composition of the present invention can be formulated as an oral or parenteral preparation to be used as a treatment and prevention agent having hair loss prevention and hair growth promoting effects.
  • composition of this invention may be orally added, it is more efficient to use it as the external preparation which uses hair growth locally in a desired site
  • the external preparation may be a cleaning agent such as shampoo or soap, or a formulation for application such as an ointment, cream, lotion, or spray.
  • the dosage of the active ingredient according to the present invention is appropriately selected depending on the absorbance of the active ingredient in the body, the type of preparation, the age, sex and condition of the patient, the degree of hair loss, etc.
  • the effective amount of saponin Rd, Re may be administered once to several times so as to be 30 ⁇ 1000 / mg. In the following examples, the effective amount was calculated based on the amount of minoxidil used, but saponins are not toxic to the human body, so even if the daily dose is increased, there is no problem.
  • the growth period / rest ratio and / or the hair growth area of the hair are notably larger than the hair regrowth agent according to the prior art, and there is no toxicity to the human body, thereby providing a safe and excellent hair regrowth agent.
  • 1 is a structural formula showing the chemical structure of saponin.
  • 2 is a graph showing the effect of saponin on the growth phase length of hair of nude mice.
  • 3 is a graph showing the effect of saponin on the growth and resting period ratio of hair of nude mice.
  • 5 is a graph showing the effect of saponin on the hair growth length of nude mice.
  • Figure 6 is a photograph showing the effect of saponin on the hair growth of nude mice.
  • FIG. 7 is a micrograph showing the effect of saponin on hair follicle tissue of nude mice.
  • a 0.2% (w / v) solution was prepared by dissolving 2 mg of saponin Rg2, Rg1, R2, Rh1, Rg3, Rb1 or Rd (Jilin University of China) in 1 ml ethanol (Ethanol, C2H5OH). Twenty-seven nude males (system name: CAnN.Cg-Foxn1nu / Crljori, Orient Bio), each weighing 20 to 24 g, were divided into nine groups of three animals each for seven experimental groups, a control group, and a comparative group.
  • the hair growth phase was applied to each of the seven experimental groups, each of the saponin solution, the control group, water, and the control group, respectively, and 40 ⁇ l of the 5% minoxidil solution were applied to the back 2 cm x 3 cm area of the nude mouse every 68 days. And the hair growth state such as the length of the rest period, hair growth area ratio, hair growth length was confirmed and the results are shown in Figs.
  • the application dose is a value converted from the application dose of minoxidil to the human body in the area ratio of the coating area.
  • FIG. 3 is a graph showing the number of days of resting and growing phases during the 68 days of observation.
  • both saponin and minoxidil were grown more than twice as much as the control water, especially in the case of Rd, the growth period was 38.2% and 22.1% was in minoxidil or other saponins corresponding to the growth period. Showed a significant effect.
  • Figure 4 is a graph showing the hair growth region of each cycle in the nude mouse Rg1, Re, Rh1 and Rd of saponin showed superior efficacy compared to minoxidil.
  • the initial hair growth area was 86%, and it was confirmed that hair growth was promoted over almost the entire area.
  • 5 is a graph showing the length of hair growth in each experimental group, most of the saponins were slightly shorter than the minoxidil compared to the control group, but showed significant efficacy compared to the control group, the difference in efficacy according to the type of saponin was not large.
  • 6 is a photograph comparing the nude mice of the Rd test group, the R2 test group and the minoxidil comparison group for each cycle.
  • the scalp tissue was observed under a microscope. More specifically, the hair growth of nude mouse skin tissue was cut to a predetermined size, and the tissue was fixed using a fixed solution in which ethanol, chloroform, and acetic acid were mixed at a volume ratio of 6: 3: 1. The fixed tissue was gradually dehydrated using 80% ethanol, 90% ethanol, and 100% ethanol in sequence, and paraffin was injected into the tissue with paraffin, which is a liquid at 60 ° C, through a transparent process of xylene. It penetrated to make a solid block.
  • the paraffin block was cut into 10 ⁇ m thickness using a microtome and attached to a glass slide, followed by HE (hematoxylin-eosinstain) staining to identify hair follicle morphology.
  • 7 is a micrograph of the scalp tissue observed for nude mice in each group.
  • the thickness of the scalp tissue was the thickest Re-administered group, it was confirmed that the thickness in the order of the minoxidil administration group, Rd administration group and the control group.
  • the scalp tissue hair follicle cells were found in the order of Re, Rd, minoxidil, and control groups, as well as the rapid growth rate, indicating that Re and Rd groups had better hair growth than the comparison group.
  • mice 21 males of 6-week-old C57BL / 6 mice (weight 16 to 18 g, oriental bio), which are most used for hair growth promoting experiments, were used in the experiment after adapting to breeding conditions for one week. The animals were maintained at optimal conditions in an independent animal laboratory until the start of the experiment.
  • the experimental group was divided into three groups of the control group (negative control group), the saponin Rd treatment group, and the minoxidil treatment group (positive control group), which were physiological saline treatment groups, and placed 7 animals in each group.
  • the animal was first removed using a clipper while the dorsal skin color was red, and the remaining hair was completely removed using a hair removal cream (Veet, Reckitt Benckiser France). .
  • the epilation cream left on the skin was wiped off with ethanol.
  • the degree of hair growth was visually observed with the progress of the date while applying the saponin Rd composition, the minoxidil composition, or the physiological saline solution, which were prepared in the same manner as in Example 1, once each day to 40 ⁇ l each using a soft brush.
  • FIG. 8 is a photograph showing the degree of hair growth for each experimental group was not observed in the saline treatment group on the 8th day, but no particular change in hair growth was observed in the minoxidil treated group and Rd treated group in some mice.
  • the negative control group showed excellent hair growth rate in the Rd-treated group and the minoxidil-treated group, whereas only some hair growth was observed.
  • the Rd-treated group of the present invention showed the fastest hair growth state.
  • hair growth was completed in all experimental groups.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Cette invention concerne une composition stimulant la croissance des cheveux contenant des saponines Rd et Re à titre de principes actifs. La composition selon la présente invention a une excellente efficacité dans la mesure où un rapport anagène/télogène des cheveux et/ou une zone de pousse des cheveux sont remarquables comparativement à un agent stimulant la croissance des cheveux de l'état de la technique. De plus, la composition peut être utilisée sans danger, n'étant pas toxique pour le corps humain.
PCT/KR2011/010274 2011-01-06 2011-12-29 Composition stimulant la croissance des cheveux contenant des saponines rd et re à titre de principes actifs WO2012093804A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110001456A KR101274029B1 (ko) 2011-01-06 2011-01-06 사포닌 Ｒｄ와 Ｒｅ를 유효성분으로 포함하는 발모용 조성물
KR10-2011-0001456 2011-01-06

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WO2012093804A2 true WO2012093804A2 (fr) 2012-07-12
WO2012093804A3 WO2012093804A3 (fr) 2012-10-18

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018534268A (ja) * 2015-09-30 2018-11-22 アモーレパシフィック コーポレーションＡｍｏｒｅｐａｃｉｆｉｃ Ｃｏｒｐｏｒａｔｉｏｎ ソヤサポニンを含有する発毛および／または育毛促進用組成物
CN116808054A (zh) * 2023-04-25 2023-09-29 三蚁科技(广州)有限公司 一种预防脱发的皂苷组合物及其制备方法和应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102028382B1 (ko) * 2012-11-07 2019-10-04 (주)아모레퍼시픽 진세노사이드를 함유하는 모발 또는 두피용 화장료 조성물
KR101957849B1 (ko) 2015-03-31 2019-03-15 (주)아모레퍼시픽 발모 또는 육모 촉진용 및 항염용 조성물

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KR100296430B1 (ko) * 1992-09-17 2001-11-05 엘뵈엠아시 러쉐르쉬 1종이상의진세노사이드계사포닌을함유하는화장료또는피부용조성물
KR20030009454A (ko) * 2000-05-31 2003-01-29 카가쿠키쥬쯔 신코지교단 진세노사이드Ｒｂ1으로 된 피부조직 재생촉진제
KR20040062379A (ko) * 2002-12-31 2004-07-07 주식회사 태평양 자기회합성을 갖는 양친성 고분자를 사용하여 난용성물질을 포집시켜 제조한 나노입자 및 이를 함유하는 피부외용제 조성물
US20070009474A1 (en) * 2005-07-08 2007-01-11 The Procter & Gamble Company Personal care compositions and methods for the beautification of mammalian skin and hair
KR20080084117A (ko) * 2007-03-15 2008-09-19 바이오스펙트럼 주식회사 진세노사이드 Ｒｏ(ｇｉｎｓｅｎｏｓｉｄｅ Ｒｏ)를함유하는 조성물

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100296430B1 (ko) * 1992-09-17 2001-11-05 엘뵈엠아시 러쉐르쉬 1종이상의진세노사이드계사포닌을함유하는화장료또는피부용조성물
KR20030009454A (ko) * 2000-05-31 2003-01-29 카가쿠키쥬쯔 신코지교단 진세노사이드Ｒｂ1으로 된 피부조직 재생촉진제
KR20040062379A (ko) * 2002-12-31 2004-07-07 주식회사 태평양 자기회합성을 갖는 양친성 고분자를 사용하여 난용성물질을 포집시켜 제조한 나노입자 및 이를 함유하는 피부외용제 조성물
US20070009474A1 (en) * 2005-07-08 2007-01-11 The Procter & Gamble Company Personal care compositions and methods for the beautification of mammalian skin and hair
KR20080084117A (ko) * 2007-03-15 2008-09-19 바이오스펙트럼 주식회사 진세노사이드 Ｒｏ(ｇｉｎｓｅｎｏｓｉｄｅ Ｒｏ)를함유하는 조성물

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018534268A (ja) * 2015-09-30 2018-11-22 アモーレパシフィック コーポレーションＡｍｏｒｅｐａｃｉｆｉｃ Ｃｏｒｐｏｒａｔｉｏｎ ソヤサポニンを含有する発毛および／または育毛促進用組成物
EP3357486A4 (fr) * 2015-09-30 2019-03-20 Amorepacific Corporation Composition favorisant la repousse et/ou la croissance des cheveux contenant de la saponine de soja
CN116808054A (zh) * 2023-04-25 2023-09-29 三蚁科技(广州)有限公司 一种预防脱发的皂苷组合物及其制备方法和应用

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WO2012093804A3 (fr) 2012-10-18
KR20120080031A (ko) 2012-07-16
KR101274029B1 (ko) 2013-06-12

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