WO2012050220A1 - Procédé et dispositif de culture de microorganismes photosynthétiques - Google Patents

Procédé et dispositif de culture de microorganismes photosynthétiques Download PDF

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Publication number
WO2012050220A1
WO2012050220A1 PCT/JP2011/073762 JP2011073762W WO2012050220A1 WO 2012050220 A1 WO2012050220 A1 WO 2012050220A1 JP 2011073762 W JP2011073762 W JP 2011073762W WO 2012050220 A1 WO2012050220 A1 WO 2012050220A1
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WO
WIPO (PCT)
Prior art keywords
gas reservoir
gas
photosynthetic
water tank
suspension
Prior art date
Application number
PCT/JP2011/073762
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English (en)
Japanese (ja)
Inventor
敏朗 関根
Original Assignee
Sekine Toshirou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekine Toshirou filed Critical Sekine Toshirou
Publication of WO2012050220A1 publication Critical patent/WO2012050220A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the present invention relates to a method and apparatus for culturing photosynthetic microorganisms such as photosynthetic bacteria and microalgae.
  • the photosynthetic microorganism culture method using sunlight outdoors is, as seen in the culture of microalgae, (1) an open pond method in which the culture is performed with stirring in a shallow flat pond, and (2) a loop-like culture.
  • the standing transparent aquarium method has the advantage of a large yield per installation area compared to the open pound method, as with the Tubular method. Because of the adhesion of microorganisms and dust, there is a drawback that they need to be removed regularly.
  • the transparent tank system with an open top has the advantage that it is easier to remove the deposits than the tubular system.
  • a cooling facility is required to prevent algae death due to overheating when culturing in low latitude areas, but the standing transparent water tank method is open at the top Therefore, there is an advantage that moisture is evaporated and the ratio of the suspension amount to the light receiving area can be increased, and no cooling equipment is required.
  • the ratio of the suspension amount to the light receiving area increases in the standing transparent water tank method, so that it is difficult to obtain a highly concentrated suspension.
  • the exhaust gas is exhausted downward in the transparent water tank.
  • A evacuating the gas in the gas reservoir, filling the photosynthetic microorganism suspension into the gas reservoir, and killing photosynthetic microorganism predatory microanimals such as rotifers under anaerobic conditions;
  • B A method for culturing photosynthetic microorganisms, characterized by comprising a step of aerating gas in the gas reservoir to raise the water level and irradiating the photosynthetic microorganism suspension with sunlight, Secondly, (a) a transparent water tank formed of a light-transmitting material for irradiating the stored photosynthetic microorganism suspension with sunlight to grow the photosynthetic microorganisms, and (a) a little at the bottom of the transparent water tank.
  • a gas reservoir provided with a ventilating mechanism installed above, (c) a substantially vertically provided pipe connecting the slightly upper end of the gas reservoir and the upper end of the gas reservoir, and (d) gas in the gas reservoir. It is the culture apparatus of the photosynthetic microorganisms characterized by including the gas press-fitting machine which supplies.
  • FIG. 1 It is a top view which shows one Embodiment of this invention. It is an AA longitudinal cross-sectional view in FIG. It is an AA longitudinal cross-sectional view in FIG. It is a longitudinal cross-sectional view which shows another embodiment. It is a longitudinal cross-sectional view which shows another embodiment. It is a longitudinal cross-sectional view which shows another embodiment.
  • 1 is a transparent water tank
  • 2 is a gas reservoir
  • 3 is an upper wall surface
  • 4 is a pipe
  • 5 is a pipe
  • 6 is an on-off valve
  • 7 is a blower
  • 8 is an on-off valve
  • 9 is a pipe
  • 10 is an on-off valve
  • 11 is a pipe
  • 12 is an on-off valve
  • 13 is a floating body
  • 14 is a perforated pipe
  • 15 is a floating body
  • 16 is a projection
  • 17 is a diffuser pipe
  • 18 is a pipe
  • 19 is a blower
  • 20 is a pipe
  • g is an air layer
  • solid arrows Indicates the direction of flow of the microalgae suspension.
  • FIG. 1 is a plan view
  • FIG. 2 is a longitudinal sectional view taken along line AA.
  • a gas reservoir 2 is provided in a cylindrical transparent water tank 1 made of a light transmitting material.
  • the gas reservoir 2 is provided such that the upper wall surface 3 is substantially horizontal, the side surface extends downward in parallel with the side surface of the transparent water tank 1, and the lower end of the side surface is positioned slightly above the bottom surface of the transparent water tank 1.
  • the on-off valve 6 is opened and the blower 7 is operated.
  • the suspension is pushed out of the gas reservoir 2 and the water level in the transparent water tank 1 rises.
  • the water level in the gas reservoir 2 gradually falls and reaches the lower end of the pipe 4, and the water level further falls below the lower end of the pipe 4 due to the surface tension of the water.
  • the pipe 4 overflows from the lower end at once, and an air layer g is formed in the pipe 4.
  • a swirling flow in the direction of the arrow is generated in the transparent water tank 1. Air escapes from the water surface in the transparent water tank 1 to the atmosphere.
  • FIG. 2 shows the situation at this time.
  • the suspension stored in the gas reservoir 2 is hardly supplied with air from the outside air, and gradually becomes anaerobic due to oxygen consumption due to the respiration of each microorganism. Survival and proliferation are limited.
  • the on-off valve 8 and the on-off valve 10 are opened, and the upper suspension is discharged by the pipe 9. Thereafter, the on-off valve 10 is closed, and water or a culture solution is added to the level of the upper wall surface 3, and the on-off valve 8 is closed. If the culture is continued while repeating the above operation every day, predation of microalgae by tiny animals such as rotifers and daphnia is restricted, and microalgae can be cultured stably and efficiently.
  • the light receiving area is 5.2 m 2 including the side surface and the water surface. From these, the ratio of the light receiving area to the installation area is 6.84, and the ratio of the light receiving area to the operation capacity is 9.29.
  • Table 1 shows a comparison between the standing transparent water tank of the present invention and the flat pond.
  • the ratio of the light receiving area to the installation area corresponds to the amount of growth per unit installation area, and the ratio of the light receiving area to the operation volume corresponds to the concentration of the suspension.
  • the ratio of the light receiving area to the installation area of the present invention is approximately 7 times that of the plane pond.
  • the ratio of the light receiving area to the operating capacity of the present invention is approximately 1.8 times that of the flat pond and the conventional standing transparent water tank.
  • the yield per unit area is large, and the concentration of the resulting suspension is higher. This leads to cost savings such as site area saving and suspension concentration.
  • the ejection size is almost constant regardless of the gas injection speed, and the stirring effect by one ejection is constant. For this reason, even if the gas injection speed is set to a small value, effective stirring can be performed, which leads to a reduction in power cost.
  • FIGS. 4 and 5 is a longitudinal sectional view showing another embodiment of the present invention. 4 corresponds to FIG. 2, and FIG. 5 corresponds to FIG.
  • a perforated tube 14 is provided at the upper end of the tube 4, a spherical floating body 15 is provided therein, and a donut-shaped floating body 13 is provided between the side surface of the transparent water tank 1 and the side surface of the gas reservoir 2,
  • the point which is provided with the protrusion 16 which prevents the floating body 13 from rising is different from the embodiment shown in FIGS.
  • the floating body 15 is located in the upper part of the perforated pipe 14, and the floating body 13 is located below the protrusion 16, and a swirling flow as indicated by an arrow is generated. .

Abstract

L'invention concerne un procédé et un dispositif de culture de microorganismes photosynthétiques qui font appel à un réservoir d'eau transparent vertical destiné à empêcher l'entrée et la reproduction d'une microfaune telle que Daphnia et Rotifera, qui s'attaquent aux microorganismes photosynthétiques. Le dispositif selon l'invention est facile à installer, permet d'obtenir une croissance importante dans la zone où il est installé, de former une suspension à densité plus élevée et de mettre en œuvre une production massive en usine. Selon le procédé et le dispositif de culture de microorganismes photosynthétiques précités, on soumet au rayonnement de la lumière solaire une suspension de microorganismes photosynthétiques contenue dans un réservoir d'eau transparent réalisé dans un matériau qui laisse passer la lumière, un réservoir de gaz équipé d'un mécanisme d'aération/décharge étant disposé dans le fond du réservoir d'eau transparent. Le procédé selon l'invention est caractérisé en ce qu'il comprend une étape (A) lors de laquelle le gaz contenu dans le réservoir de gaz est déchargé et le réservoir de gaz est rempli avec la suspension de microorganismes photosynthétiques, la microfaune prédatrice des microorganismes photosynthétiques telle que Rotifera étant tuée par les conditions anaérobies, et une étape (B) lors de laquelle le gaz est introduit par barbotage dans le réservoir de gaz, élevant le niveau de l'eau, et la suspension de microorganismes photosynthétiques est soumise aux rayons de la lumière solaire.
PCT/JP2011/073762 2010-10-13 2011-10-07 Procédé et dispositif de culture de microorganismes photosynthétiques WO2012050220A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010-244626 2010-10-13
JP2010244626A JP2012080864A (ja) 2010-10-13 2010-10-13 光合成微生物の培養方法及び装置

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WO2012050220A1 true WO2012050220A1 (fr) 2012-04-19

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094721A (zh) * 2017-07-10 2017-08-29 佛山市水创科联生态科技有限公司 野外原生大型溞富集装置以及富集方法
WO2022196271A1 (fr) * 2021-03-15 2022-09-22 本田技研工業株式会社 Dispositif de culture

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101413060B1 (ko) 2012-08-03 2014-07-31 그린파이너스(주) 물벼룩 배양 장치

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0690735A (ja) * 1992-09-08 1994-04-05 Toshiro Sekine 光合成微生物の培養装置とその運転方法
WO1998029531A1 (fr) * 1996-12-30 1998-07-09 Toshirou Sekine Appareil de culture des microalgues
WO2003006629A1 (fr) * 2001-07-11 2003-01-23 Toshirou Sekine Procede et dispositif de culture de microbes de photosynthese
JP2003199455A (ja) * 2002-01-08 2003-07-15 Tetra Co Ltd 微細藻類の増殖方法及び増殖装置
JP2006223280A (ja) * 2005-02-15 2006-08-31 Clean System:Kk 光合成細菌をステビアで培養する方法と培養された水溶液
WO2010038912A2 (fr) * 2008-10-03 2010-04-08 Sekine Toshirou Procédé et dispositif de culture de micro-algues
JP2011087552A (ja) * 2009-10-17 2011-05-06 Toshiro Sekine 微細藻類培養方法及び装置

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0690735A (ja) * 1992-09-08 1994-04-05 Toshiro Sekine 光合成微生物の培養装置とその運転方法
WO1998029531A1 (fr) * 1996-12-30 1998-07-09 Toshirou Sekine Appareil de culture des microalgues
WO2003006629A1 (fr) * 2001-07-11 2003-01-23 Toshirou Sekine Procede et dispositif de culture de microbes de photosynthese
JP2003199455A (ja) * 2002-01-08 2003-07-15 Tetra Co Ltd 微細藻類の増殖方法及び増殖装置
JP2006223280A (ja) * 2005-02-15 2006-08-31 Clean System:Kk 光合成細菌をステビアで培養する方法と培養された水溶液
WO2010038912A2 (fr) * 2008-10-03 2010-04-08 Sekine Toshirou Procédé et dispositif de culture de micro-algues
JP2011087552A (ja) * 2009-10-17 2011-05-06 Toshiro Sekine 微細藻類培養方法及び装置

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094721A (zh) * 2017-07-10 2017-08-29 佛山市水创科联生态科技有限公司 野外原生大型溞富集装置以及富集方法
CN107094721B (zh) * 2017-07-10 2021-01-26 广东民信科技发展有限公司 野外原生大型溞富集装置以及富集方法
WO2022196271A1 (fr) * 2021-03-15 2022-09-22 本田技研工業株式会社 Dispositif de culture

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